Grosvenor momordica fruit purification chromatographic column

Abstract

The utility model discloses a momordica grosvenori purification chromatographic column which comprises a chromatographic column body, a sealing cover is arranged at the top of the chromatographic column body, a mother liquor inlet pipe, an eluent inlet pipe, a steam input pipe and a vacuumizing pipe are arranged on the sealing cover, and a vacuum pump is connected to the vacuumizing pipe. The liquid inlet end of the mother liquid inlet pipe is sequentially connected with a first mixing pipe, a mixing section and a second mixing pipe, the liquid inlet end of the second mixing pipe is provided with a shunt pipe, the liquid inlet end of the shunt pipe is respectively connected with a mother liquid input pipe and a buffer liquid input pipe, the input end of the steam input pipe is connected with a steam generator, and the bottom end of the chromatographic column body is provided with a liquid discharge pipe. The siraitia grosvenorii mother liquor and the buffer solution can be autonomously mixed in the liquor feeding process, so that the uniformity of the siraitia grosvenorii mother liquor during chromatographic purification is improved, and meanwhile, the chromatographic purification efficiency can be improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to monk fruit processing technical field more specifically, it relates to a monk fruit purification chromatographic column. BACKGROUND

[0002] Siraitia grosvenorii, also known as "God's fruit" or "longevity fruit", is a kind of cucurbitaceae plant originally from Guangxi Zhuang Autonomous Region, China. Its fruit is famous for its extremely high sweetness and medicinal value, and is an important ingredient in traditional Chinese medicine and modern health food.

[0003] The Chinese patent document with publication number CN208130559U discloses a monk fruit purification chromatographic column, which sets stirring rods and stirring teeth in the chromatographic column to realize the sufficient mixing between mother liquor and buffer chemicals during the monk fruit purification chromatography process. However, during the chromatography separation process, the solid phase needs to be kept relatively stable. The above stirring method can easily affect the balance inside the chromatographic column, thereby affecting the purification and separation effect of monk fruit.

[0004] In addition, the above stirring method can easily mix the pores inside the chromatographic column into the monk fruit mother liquor and buffer solution. During the chromatography separation process, it is necessary to avoid bubbles in the liquid. The above method can affect the chromatography separation and purification effect of monk fruit. UTILITY MODEL CONTENT

[0005] (I) Technical problem solved

[0006] In view of the problems existing in the prior art, the utility model provides a monk fruit purification chromatographic column to solve the technical problem of poor purification and separation effect of the monk fruit purification chromatographic column in the prior art during the chromatography separation and purification process of monk fruit.

[0007] (II) Technical scheme

[0008] To achieve the above-mentioned purpose, the utility model provides the following technical scheme:

[0009] A monk fruit purification chromatographic column, comprising a chromatographic column body, a sealing cover is arranged at the top of the chromatographic column body, a mother liquor inlet pipe, an eluent inlet pipe, a steam input pipe and a vacuum pump are arranged on the sealing cover, a vacuum pump is connected to the vacuum pump, a first mixing pipe, a mixing section and a second mixing pipe are sequentially connected to the inlet end of the mother liquor inlet pipe, a shunt pipe is arranged at the inlet end of the second mixing pipe, the inlet end of the shunt pipe is connected to a mother liquor input pipe and a buffer solution input pipe respectively, a steam generator is connected to the input end of the steam input pipe, and a liquid outlet pipe is arranged at the bottom end of the chromatographic column body.

[0010] The utility model further sets up, install the post in first mixed pipe and second mixed pipe, spiral mixing blade is established on install the post, the periphery of spiral mixing blade is connected fixed with the inner wall of corresponding first mixed pipe and second mixed pipe, and spiral mixing flow channel is formed, and momordica grosvenori mother liquor and buffer solution are transported to second mixed pipe way inside simultaneously, so that can make two kinds of liquid in the process of flowing in spiral mixing flow channel, realize independent mixing, provide liquid inlet uniformity, can further improve the mixing effect between momordica grosvenori mother liquor and buffer solution through the setting of first mixed pipe.

[0011] The utility model further sets up, the mixed section is connected with first mixed pipe and second mixed pipe and is opened with mixing chamber, and mixing vane is installed in mixing chamber through bearing, and the liquid that enters mixing chamber can drive mixing vane to rotate, thereby can improve the mixing uniformity of momordica grosvenori mother liquor and buffer solution when liquid inlet.

[0012] The utility model further sets up, the outside of chromatography column body is equipped with heating jacket, and electric heating wire is set up in heating jacket, and the mother liquor liquid in chromatography column body can be heated through electric heating wire, guarantees the purification temperature of momordica grosvenori, and here electric heating wire can be used in cooperation with temperature controller, temperature detector and other detection, control element, and this is prior art known technology, and the utility model does not repeat the specific use principle and circuit structure.

[0013] The utility model further sets up, and the inside bottom end of chromatography column body is provided with solid phase filling layer, which is used for realizing chromatography purification of momordica grosvenori.

[0014] The utility model further sets up, and the inside bottom end of chromatography column body is provided with solid phase filling layer, which is used for realizing chromatography purification of momordica grosvenori.

[0015] The utility model further sets up, and the inside bottom end of chromatography column body is provided with solid phase filling layer, which is used for realizing chromatography purification of momordica grosvenori.

[0016] The utility model further sets up, and the inside bottom end of chromatography column body is provided with solid phase filling layer, which is used for realizing chromatography purification of momordica grosvenori.

[0017] (Three) beneficial effects

[0018] Compared with the prior art, the utility model provides a momordica grosvenori purification chromatography column, has the following

[0019] Advantages:

[0020] 1、The utility model discloses a mother liquor liquid inlet pipe is set up, and the first mixed pipe, mixed section and second mixed pipe that cooperate with each other are set up at the liquid inlet end of mother liquor liquid inlet pipe, and the tail end of second mixed pipe is connected with mother liquor input pipe and buffer solution input pipe through the shunt pipe respectively, when using, need to send the mother liquor of momordica grosvenori and buffer solution to the second mixed pipe under the action of valve body according to the appropriate proportion, and realize the independent mixing in the spiral mixing flow passage in the second mixed pipe during the conveying process, then, enter the mixed section, and drive the mixing impeller in the mixing chamber to rotate independently, thereby can further improve the mixing sufficiency between mother liquor and buffer solution, then, the mixed liquid of the mother liquor and buffer solution after mixing will enter the second mixed pipe, and under the action of the spiral mixed liquid sheet in the second mixed pipe, further mix, thereby can improve the uniformity of mother liquor when entering the inside of chromatography column body, thereby can avoid the influence of momordica grosvenori mother liquor on the inside solid phase stability of chromatography column body when stirring and mixing, improve the purification effect when momordica grosvenori chromatography purification.

[0021] 2、Meanwhile, by the above-mentioned mode, the mixing between the mother liquor of momordica grosvenori and buffer solution can be realized during the liquid inlet process, the situation that the bubbles enter the liquid of chromatography is avoided by avoiding the stirring mode in the prior art, and the purification effect in the momordica grosvenori chromatography purification process is improved.

[0022] 3、Meanwhile, the utility model discloses a vacuum pump is set up on the sealing cover and vacuum pipe, so, before liquid inlet, the air in the chromatography column can be extracted through the cooperation of vacuum pump and vacuum pipe, thereby can further avoid the bubbles mixed in the liquid inlet process, and during the chromatography process, clean gas is transported to the inside of chromatography column body through vacuum pump and vacuum pipe, thereby increase the air pressure in the inside of chromatography column body, and improve the efficiency when momordica grosvenori liquid chromatography in the inside of chromatography column body.

[0023] 4、After chromatography, eluent can be inputted to elute through eluent liquid inlet pipe, the utility model discloses a steam input pipe is set up, can input high-temperature steam to the inside of chromatography column body under the action of steam generator and steam input pipe after use, thereby can carry out high-temperature sterilization to the inside of chromatography column body, and avoid bacterial pollution during chromatography. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 It is the whole structure diagram of a momordica grosvenori purification chromatography column in the utility model Figure 1 ;

[0025] Figure 2 It is the whole structure diagram of a momordica grosvenori purification chromatography column in the utility modelFigure 2 ;

[0026] Figure 3 It is the internal structure cross section view schematic diagram between the first mixing pipe, the mixed section and the second mixing pipe in the utility model;

[0027] Figure 4 It is the internal structure cross section view schematic diagram of the chromatographic column body.

[0028] In the drawing: 1, chromatographic column body; 2, sealing cover; 3, mother liquor inlet pipe; 4, eluent inlet pipe; 5, steam input pipe; 6, vacuum extraction pipe; 7, vacuum pump; 8, first mixing pipe; 9, mixed section; 10, second mixing pipe; 11, shunt pipe; 12, mother liquor input pipe; 13, buffer solution input pipe; 14, steam generator; 15, liquid discharge pipe; 16, mounting column; 17, spiral mixing blade; 18, spiral mixing runner; 19, mixing cavity; 20, mixing impeller; 21, heating jacket; 22, electric heating wire; 23, solid phase filling layer; 24, flow guide pipe; 25, valve; 26, liquid discharge valve. DETAILED DESCRIPTION

[0029] It should be noted that the embodiments in the present application and the features in the embodiments can be combined with each other without conflict. The utility model will be described in detail below with reference to the drawings and in combination with the embodiments.

[0030] It should be noted that, unless otherwise specified, all technical and scientific terms used in the present application have the same meaning as generally understood by those skilled in the art to which the present application belongs.

[0031] In the utility model, unless otherwise stated, the orientation such as "up, down" is generally for the direction shown in the drawing, or for the vertical, perpendicular or gravity direction; similarly, for the convenience of understanding and description, "left, right" is generally for the left and right shown in the drawing; "inner, outer" refers to the inner and outer of the contour of each component itself, but the above orientation words are not used to limit the utility model.

[0032] Please refer to Figures 1-4 A luohanguo purification chromatographic column, comprising a chromatographic column body 1, a sealing cover 2 is arranged at the top of the chromatographic column body 1, a mother liquor inlet pipe 3, an eluent inlet pipe 4, a steam input pipe 5 and a vacuum extraction pipe 6 are arranged on the sealing cover 2, a vacuum pump 7 is connected to the vacuum extraction pipe 6, a first mixing pipe 8, a mixed section 9 and a second mixing pipe 10 are sequentially connected to the liquid inlet end of the mother liquor inlet pipe 3, a shunt pipe 11 is arranged at the liquid inlet end of the second mixing pipe 10, a mother liquor input pipe 12 and a buffer solution input pipe 13 are respectively connected to the liquid inlet end of the shunt pipe 11, a steam generator 14 is connected to the input end of the steam input pipe 5, and a liquid discharge pipe 15 is arranged at the bottom end of the chromatographic column body 1.

[0033] Please see Figures 1-4 As one embodiment of the first mixing tube 8 and the second mixing tube 10: each of the first mixing tube 8 and the second mixing tube 10 is provided with a mounting column 16, and a spiral mixing blade 17 is provided on the mounting column 16. The outer periphery of the spiral mixing blade 17 is connected and fixed to the inner wall of the corresponding first mixing tube 8 and the second mixing tube 10, and a spiral mixing channel 18 is formed. The monk fruit mother liquor and the buffer solution are synchronously transported into the channel of the second mixing tube 10, so that the two liquids can achieve self-mixing during the flow in the spiral mixing channel 18, providing uniformity of liquid inlet. The setting of the first mixing tube 8 can further improve the mixing effect between the monk fruit mother liquor and the buffer solution.

[0034] Please see Figures 1-4 As one embodiment of the mixing section 9: a mixing chamber 19 is provided in the mixing section 9, which connects the first mixing pipe 8 and the second mixing pipe 10. A mixing impeller 20 is installed in the mixing chamber 19 through a bearing. The liquid entering the mixing chamber 19 will drive the mixing impeller 20 to rotate, thereby improving the mixing uniformity between the monk fruit mother liquor and the buffer solution during liquid inlet.

[0035] Please see Figures 1-4 As one embodiment of the chromatography column body 1: a heating sleeve 21 is provided on the outer side of the chromatography column body 1, and an electric heating wire 22 is provided inside the heating sleeve 21. The mother liquor liquid in the chromatography column body 1 can be heated by the electric heating wire 22 to ensure the purification temperature of Luo Han Guo. Here, the electric heating wire 22 can be used in conjunction with temperature controllers, temperature detectors and other detection and control elements. This is the existing known technology, and the specific use principle and circuit structure will not be described in detail in this utility model.

[0036] Please see Figures 1-4 As one embodiment of the chromatography column body 1: a solid phase packing layer 23 is provided at the bottom of the internal interior of the chromatography column body 1 for the chromatography purification of monk fruit.

[0037] Please see Figures 1-4 As one embodiment of the mother liquor inlet pipe 3: the mother liquor inlet pipe 3 extends into the interior of the chromatography column body 1 and is provided with a guide pipe 24. The bottom end of the guide pipe 24 is attached to the inner wall of the chromatography column body 1. The liquid through the mother liquor inlet pipe 3 can flow downward along the inner wall of the chromatography column body 1 through the guide pipe 24, thereby improving the stability of the liquid when it enters the interior of the chromatography column body 1 and avoiding the introduction of air bubbles.

[0038] Please see Figures 1-4As an embodiment of the chromatography column, the mother liquor input pipe 12, the buffer input pipe 13, the eluent input pipe 4 and the steam input pipe 5 are provided with valves 25, so as to control the opening and closing of the mother liquor input pipe 12, the buffer input pipe 13, the eluent input pipe 4 and the steam input pipe 5.

[0039] As an embodiment of the chromatography column, the mother liquor input pipe 12, the buffer input pipe 13, the eluent input pipe 4 and the steam input pipe 5 are provided with valves 25, so as to control the opening and closing of the mother liquor input pipe 12, the buffer input pipe 13, the eluent input pipe 4 and the steam input pipe 5. Figures 1-4 As an embodiment of the chromatography column, the mother liquor input pipe 12, the buffer input pipe 13, the eluent input pipe 4 and the steam input pipe 5 are provided with valves 25, so as to control the opening and closing of the mother liquor input pipe 12, the buffer input pipe 13, the eluent input pipe 4 and the steam input pipe 5.

[0040] As an embodiment of the chromatography column, the mother liquor input pipe 12, the buffer input pipe 13, the eluent input pipe 4 and the steam input pipe 5 are provided with valves 25, so as to control the opening and closing of the mother liquor input pipe 12, the buffer input pipe 13, the eluent input pipe 4 and the steam input pipe 5.

[0041] In use, the buffer solution can be delivered into the chromatography column body 1 through the buffer input pipe 13, and the chromatography column body 1 is infiltrated with the buffer solution.

[0042] Then, the mother liquor and the buffer solution are delivered into the second mixing pipe 10 in a proper ratio under the action of the valve body, and are mixed independently in the spiral mixing flow channel 18 in the second mixing pipe 10.

[0043] Then, the mixed solution enters the mixing section 9, and the mixing impeller 20 in the mixing chamber 19 is driven to rotate independently, so that the mixing of the mother liquor and the buffer solution is further improved.

[0044] Then, the mixed solution of the mother liquor and the buffer solution after mixing enters the second mixing pipe 10, and is further mixed under the action of the spiral mixing flow channel in the second mixing pipe 10, so that the uniformity of the mother liquor when entering the chromatography column body 1 is improved, and the influence of the mother liquor of the momordica grosvenori on the stability of the solid phase in the chromatography column body 1 is avoided, and the purification effect of the momordica grosvenori chromatography is improved.

[0045] Meanwhile, the above-mentioned way makes the liquid mixed between the mother liquor and the buffer solution in the liquid inlet process, avoids the situation that the bubbles enter the liquid in the chromatography, and improves the purification effect in the momordica grosvenori chromatography process.

[0046] Meanwhile, the vacuum pump 7 and the vacuum pipe 6 are arranged on the sealing cover 2, so that the air in the chromatography column can be extracted through the cooperation of the vacuum pump 7 and the vacuum pipe 6 before the liquid is input, and the bubbles mixed in the liquid inlet process are further avoided.

[0047] After the chromatography is completed, the eluent can be input through the eluent inlet pipe 4, the utility model discloses a steam input pipe 5 can be set up, can input high temperature steam to the inside of chromatographic column body 1 under the action of steam generator 14 and steam input pipe 5 after using, thereby can carry out high temperature sterilization to the inside of chromatographic column body 1, avoid bacterial contamination in the chromatography process.

[0048] In all the above-mentioned solutions, the connection between the two components can be selected according to the actual situation, such as welding, bolt and nut connection, bolt or screw connection, or other known connection methods, which will not be described here. In the above, whenever there is a fixed connection, welding is preferred. Although embodiments of the utility model have been shown and described, those skilled in the art can understand that various changes, modifications, replacements and deformations can be made to these embodiments without departing from the principles and spirits of the utility model. The scope of the utility model is defined by the appended claims and their equivalents.

[0049] In all the above-mentioned solutions, if there is no explicit description, the electrical element is controlled by the controller. Since the controller is matched with common equipment, its control principle and circuit connection belong to existing known and mature technology, and its specific circuit structure will not be described here.

[0050] In all the above-mentioned solutions, if the motor is actually needed, it can be matched with a speed reducer. The connection structure and working principle between the motor and the speed reducer are existing known technology, and the utility model will not be described here.

[0051] In all the above-mentioned solutions, if the solar panel is connected with the battery, it can be matched with an inverter, a battery charging controller, a cable, a fuse and a support, etc. Its control principle and circuit connection belong to existing known and mature technology, and its specific circuit structure will not be described here.

Claims

1. A purified luohanguo chromatography column, comprising a chromatography column body (1), the top of the chromatography column body (1) is provided with a sealing cover (2), characterized in that: The sealing cover (2) is provided with mother liquor inlet pipe (3), eluent inlet pipe (4), steam input pipe (5) and vacuum pipe (6), the vacuum pump (7) is connected on the vacuum pipe (6), the inlet end of the mother liquor inlet pipe (3) is sequentially connected with first mixing pipe (8), mixing section (9) and second mixing pipe (10), the inlet end of the second mixing pipe (10) is provided with shunt pipe (11), the inlet end of the shunt pipe (11) is respectively connected with mother liquor input pipe (12) and buffer solution input pipe (13), the input end of the steam input pipe (5) is connected with steam generator (14), the bottom end of the chromatography column body (1) is provided with drain pipe (15).

2. The column for purifying Momordica grosvenori according to claim 1, wherein the column is characterized in that: The first mixing pipe (8) and the second mixing pipe (10) are provided with mounting column (16), the mounting column (16) is provided with spiral mixing blade (17), the outer periphery of the spiral mixing blade (17) is connected and fixed with the inner wall of the first mixing pipe (8) and the second mixing pipe (10), and the spiral mixing flow channel (18) is formed.

3. The column according to claim 2, wherein the column is characterized by: The mixing section (9) is provided with mixing cavity (19) communicated with the first mixing pipe (8) and the second mixing pipe (10), and the mixing impeller (20) is installed in the mixing cavity (19) through bearing.

4. The column according to claim 1, wherein the column is characterized by: The outer side of the chromatography column body (1) is provided with heating jacket (21), and the inside of the heating jacket (21) is provided with electric heating wire (22).

5. The column according to claim 1, wherein the column is characterized by: The inside bottom end of the chromatography column body (1) is provided with solid phase filling layer (23).

6. The column according to any one of claims 1-5, wherein the column is characterized in that: The mother liquor inlet pipe (3) extending to the inside of the chromatography column body (1) is provided with flow guide pipe (24), and the bottom end of the flow guide pipe (24) is attached to the inner wall of the chromatography column body (1).

7. The column according to claim 1, wherein the column is characterized by: The mother liquor input pipe (12), buffer solution input pipe (13), eluent inlet pipe (4) and steam input pipe (5) are provided with valve (25).

8. The column according to claim 1, wherein the column is characterized by: The drain pipe (15) is provided with drain valve (26).

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