Micro liquid sample storage and taking container

Abstract

The utility model discloses a trace liquid sample storage and taking container which comprises a sample storage unit for directly containing a liquid sample, a sample taking unit arranged at the input end of the sample storage unit, and an injection head protection unit arranged at the output end of the sample storage unit, the sample storage unit comprises a first columnar base body, a sample storage cavity and a micro injection pipe. According to the utility model, the principle of an injector is ingeniously applied, a split detachable connecting system is designed, and all units form a closed container after being assembled, so that the sample transportation is convenient; the technical defect of liquid residue in the traditional taking process is eliminated by adopting a sampling mode of pushing out a micro sample from a micro injection pipe below the sample storage cavity, so that the utilization rate of the sample is improved; in addition, a bucket-shaped input opening is formed in the upper portion of the sample storage cavity, so that sample injection is facilitated.

Description

TECHNICAL FIELD

[0001] The embodiment of the utility model belongs to the technical field of sample storage container, more particularly, relate to a kind of trace liquid sample storage and access container. BACKGROUND

[0002] In biomedical research, the collection of aqueous humor has important significance for the study of ophthalmic diseases and drug development. Aqueous humor is the liquid between the anterior chamber and the posterior chamber of the eye, and its composition and properties can reflect the physiological and pathological state of the eye. Effective collection and storage of aqueous humor is one of the key links in the study, and the sample acquisition is extremely small, usually in microliter level. In clinical application, the storage operation of trace aqueous humor is not standardized, and accidents such as loss and contamination are easily caused. Therefore, it is urgent to provide a trace liquid sample storage and access method to ensure the safe and effective access of trace valuable samples.

[0003] In order to solve the above technical problems, a trace sperm cryopreservation method of Chinese invention patent CN108207931B aims to provide a cryopreservation carrier and method for trace sperm (containing single sperm, such as testicular puncture sperm, epididymal puncture sperm, sperm of patients with severe oligospermia and asthenospermia, etc.) with small volume, closed space, easy operation and high sperm activity. When sperm is recovered, the cryopreservation carrier is taken out from the cryopreservation tube with a pipette, and is quickly added to the sperm culture dish (37℃). Under the inverted microscope (100-200 times magnification), the sperm is carefully searched in the cryopreservation carrier, and is transferred into the sperm culture medium of the sperm culture dish with a single sperm injection needle in the follicle. It is used for subsequent sperm immobilization and ICSI of matured oocytes.

[0004] In the above patent technology, a special cryopreservation tube or culture dish is used to assist in storing 1.8ml sample, but there are still the following technical problems: (1) The microliter level sample solution is added to the ordinary structure type test tube or culture dish, and the sample solution is easily left on the wall during the subsequent access process, causing waste; (2) If the ordinary structure type test tube is usually in open form, it brings inconvenience to the transportation process of trace sample; (3) A special trace storage and access container should be considered to design, so as to realize the technical effects of convenient injection, complete removal and convenient transportation. UTILITY MODEL CONTENTS

[0005] In view of the above defects or improvement needs of the prior art, the utility model provides a trace liquid sample storage and taking container. The syringe principle is ingeniously used, a split detachable connection system is designed, each unit is assembled into a sealed container, and sample transportation is facilitated; a sampling mode of pushing the trace sample out from the fine injection pipe below the sample storage cavity is adopted, technical defects of liquid residue in the traditional taking process are eliminated, and sample utilization is improved; in addition, a bucket type input port is arranged above the sample storage cavity, and sample injection is facilitated.

[0006] In order to realize the above-mentioned purpose, a trace liquid sample storage and taking container comprises:

[0007] A sample storage unit for directly containing liquid samples, a sample taking unit arranged at the input end of the sample storage unit, and an injection head protection unit arranged at the output end of the sample storage unit;

[0008] The sample storage unit comprises a first column type base, a sample storage cavity vertically arranged in the first column type base, a fine injection pipe arranged at the output end of the sample storage cavity, an interface arranged at the output end of the sample storage cavity, a first connection thread opened in the inner side wall of the interface, a first connection ring and a rubber sealing protrusion arranged on the lower surface of the first column type base, and a connection thread arranged on the outer side wall of the first connection ring; the connection thread on the outer side wall of the first connection ring is used for connecting the injection head protection unit, the rubber sealing protrusion is in close contact with the injection head protection unit to realize the air-tight effect around the fine injection pipe, and the first connection thread is used for connecting the sample taking unit;

[0009] The sample taking unit comprises a piston injection assembly used for pressurizing the sample storage cavity to push the sample downward from the fine injection pipe, and a trigger prevention cover arranged at the back of the piston injection assembly to place the piston and prevent mispushing.

[0010] Preferably, the inlet of the sample storage cavity is a bucket type input port.

[0011] Preferably, the outlet of the sample storage cavity is a tapered output port.

[0012] Preferably, a sealing band is arranged between the contact surface of the sample storage unit and the sample taking unit.

[0013] Preferably, the piston injection assembly comprises:

[0014] A second column type base, a piston sliding cavity arranged on the second column type base, a piston and a push rod sliding in the piston sliding cavity, a second connection ring arranged on the lower surface of the second column type base, and a second connection thread arranged on the outer side surface of the second connection ring and threadedly connected with the first connection thread.

[0015] A third connecting ring arranged on the upper surface of the second cylindrical base body, and a third connecting thread arranged on the outer side surface of the third connecting ring, the third connecting thread being used for connecting the anti-trigger cover.

[0016] Preferably, the anti-trigger cover comprises:

[0017] A third cylindrical base body, a fourth connecting thread arranged on the inner side wall of the third cylindrical base body, and an anti-skid surface arranged on the outer side surface of the third cylindrical base body; the fourth connecting thread is threadedly fixedly connected with the third connecting thread.

[0018] Preferably, the injection head protection unit comprises:

[0019] A fourth cylindrical base body, a protection cavity arranged in the fourth cylindrical base body for inserting and placing a protection micro-fine injection pipe, a connecting ring groove and a sealing alignment ring groove arranged on the upper surface of the fourth cylindrical base body, and a fifth connecting thread arranged on the inner side surface of the connecting ring groove.

[0020] The fifth connecting thread is connected with the outer side surface connecting thread of the first connecting ring, and the sealing alignment ring groove is tightly and clampingly connected with the rubber sealing protrusion.

[0021] Preferably, the rotation directions of the first connecting thread and the second connecting thread are opposite to the rotation directions of the third connecting thread and the fourth connecting thread.

[0022] Overall, compared with the prior art, the above technical scheme of the utility model can achieve the following beneficial effects:

[0023] The micro-liquid sample storage and taking container of the utility model ingeniously uses the principle of a syringe, designs a split detachable connection system, and is a closed container after total assembly of each unit, thereby facilitating sample transportation; the sample taking mode of pushing the micro sample out of the micro-fine injection pipe below the sample storage cavity is adopted, thereby eliminating the technical defect of liquid residue in the traditional taking process, and thereby improving sample utilization rate; in addition, the sample storage cavity is provided with a bucket-shaped input port above the sample storage cavity, thereby facilitating sample injection. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 It is a whole cross-sectional structure schematic view of the micro-liquid sample storage and taking container of the utility model embodiment;

[0025] Figure 2 It is an explosion structure schematic view of the micro-liquid sample storage and taking container of the utility model embodiment;

[0026] Figure 3 It is a first use state view of the micro-liquid sample storage and taking container of the utility model embodiment;

[0027] Figure 4 Figure 2 is a second use state diagram of a trace liquid sample storage and taking container according to an embodiment of the present application;

[0028] Figure 5 Figure 3 is a third use state diagram of a trace liquid sample storage and taking container according to an embodiment of the present application;

[0029] In all the drawings, the same reference signs indicate the same technical features, specifically: 1-sample storage unit, 100-first columnar base body, 101-sample storage cavity, 102-tapered output port, 103-bucket-shaped input port, 104-fine injection pipe, 111-first connecting ring, 112-rubber sealing protrusion, 113-interfacing port, 114-first connecting thread, 115-sealing strip, 2-sample taking unit, 210-piston injection assembly, 211-second columnar base body, 212-handle, 213-second connecting ring, 214-second connecting thread, 215-piston sliding cavity, 216-piston, 217-push rod, 218-third connecting ring, 219-third connecting thread, 220-anti-trigger cover body, 221-third columnar base body, 222-fourth connecting thread, 223-anti-slip surface, 3-injection head protection unit, 300-fourth columnar base body, 301-protection cavity, 302-connecting ring groove, 303-fifth connecting thread, 304-sealing alignment ring groove. DETAILED DESCRIPTION

[0030] In the description of the present application, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise" and the like indicate the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0031] In addition, the terms "first", "second" are only for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined with "first", "second" can explicitly or implicitly include one or more of the features. In the description of the present application, the meaning of "multiple" is two or more, unless otherwise specifically limited.

[0032] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0033] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only for explaining the present utility model and are not intended to limit the present utility model. Furthermore, the technical features involved in the various embodiments of the present utility model described below can be combined with each other as long as they do not conflict with each other.

[0034] like Figures 1-5 As shown in this embodiment of the invention, the micro-liquid sample storage and retrieval container includes:

[0035] A sample storage unit 1 for directly holding liquid samples, a sample retrieval unit 2 located at the input end of the sample storage unit 1, and a dispensing head protection unit 3 located at the output end of the sample storage unit 1;

[0036] The sample storage unit 1 includes a first cylindrical substrate 100, a sample storage cavity 101 vertically penetrating the first cylindrical substrate 100, a micro-injection tube 104 at the output end of the sample storage cavity 101, an interface 113 at the output end of the sample storage cavity 101, a first connecting thread 114 formed on the inner sidewall of the interface 113, a first connecting ring 111 and a rubber sealing protrusion 112 on the lower surface of the first cylindrical substrate 100, and a connecting thread on the outer sidewall of the first connecting ring 111; the connecting thread on the outer sidewall of the first connecting ring 111 is used to connect the injection head protection unit 3, the rubber sealing protrusion 112 is in close contact with the injection head protection unit 3 to achieve an airtight effect around the micro-injection tube 104, and the first connecting thread 114 is used to connect the sample retrieval unit 2.

[0037] The sample retrieval unit 2 includes a piston injection assembly 210 for pressurizing the sample storage cavity 101 to push the sample downward from the micro-injection tube 104, and an anti-trigger cover 220 disposed on the back of the piston injection assembly 210 to prevent accidental piston push.

[0038] like Figures 1-5 As shown in this embodiment of the present invention, the inlet of the sample storage cavity 101 is a bucket-shaped input port 103.

[0039] As Figures 1-5 shown in the utility model embodiment, the sample storage cavity 101 outlet is tapered outlet 102.

[0040] As Figures 1-5 shown in the utility model embodiment, the sample storage unit 1 and sample access unit 2 contact surface are equipped with sealing tape 115 between.

[0041] As Figure 2 shown in the utility model embodiment, the piston injection assembly 210 includes:

[0042] Second cylindrical base body 211, the piston sliding cavity 215 of being equipped with in second cylindrical base body 211, the piston 216 and push rod 217 sliding in the piston sliding cavity 215, the second connecting ring 213 of being equipped with in the lower surface of second cylindrical base body 211, the second connecting thread 214 of being equipped with in the outer side surface of second connecting ring 213 and the first connecting thread 114 keeps screw thread connection.

[0043] The third connecting ring 218 of being equipped with in the upper surface of second cylindrical base body 211, the third connecting thread 219 of being equipped in the outer side surface of third connecting ring 218, the third connecting thread 219 is used to connect the anti-trigger cover 220.

[0044] As Figure 2 shown in the utility model embodiment, the anti-trigger cover 220 includes:

[0045] Third cylindrical base body 221, fourth connecting thread 222 being opened in the inner side wall of third cylindrical base body 221, anti-skid surface 223 being equipped in the outer side surface of third cylindrical base body 221, the fourth connecting thread 222 and the third connecting thread 219 keep screw fixed connection.

[0046] As Figure 2 shown in the utility model embodiment, the injection head protection unit 3 includes:

[0047] Fourth cylindrical base body 330, protection cavity 301 being opened in fourth cylindrical base body 330 to insert and place protection micro-injection pipe 104, connecting ring groove 302 and sealing alignment ring groove 304 being equipped in the upper surface of fourth cylindrical base body 330, fifth connecting thread 303 being equipped in the inner side surface of connecting ring groove 302.

[0048] The fifth connecting thread 303 and the outer side surface connecting thread of first connecting ring 111 keep connection, and the sealing alignment ring groove 304 and rubber sealing lug 112 are tightly clamped and connected.

[0049] As Figure 2As shown in the embodiment of the utility model, the rotation direction of the first connecting thread 114 and the second connecting thread 214 is opposite to the rotation direction of the third connecting thread 219 and the fourth connecting thread 222.

[0050] The utility model working principle:

[0051] S100: as Figure 3 As shown, in the initial state, the sample taking unit 2 is in a separated state, and the injection head protection unit 3 and the sample storage unit 1 remain connected and sealed state;The micro sample is injected from the bucket-shaped input port 103 to the sample storage cavity 101, at this time, because the injection head protection unit 3 and the sample storage unit 1 are in airtight state, under the action of air pressure balance, the micro solution sample will not flow out from the micro injection pipe 104 below the sample storage cavity 101;

[0052] S200: as Figure 4 As shown, after filling, the outer surface of the piston injection assembly 210 is rotated in the positive direction to rotate the sample taking unit 2 in the opening of the sample storage unit 1, and under the action of the sealing strip 115, the airtight seal between the two is realized, so that the sample is preserved for transportation;

[0053] S300: as Figure 5 As shown, when taking, keep the same positive rotation direction, rotate the outer surface of the anti-trigger cover 220, because the rotation direction of the first connecting thread 114 and the second connecting thread 214 is opposite to the rotation direction of the third connecting thread 219 and the fourth connecting thread 222, so not only can the piston injection assembly 210 and the anti-trigger cover 220 be separated, but also the fastening force between the piston injection assembly 210 and the sample storage unit 1 is further tightened;At the same time, the injection head protection unit 3 is rotated away;Then the piston can be pressed, and the liquid in the sample storage cavity 101 is pushed out from the micro injection pipe 104 below, realizing the sample taking effect without liquid residue.

[0054] In the embodiment of the utility model, the principle of syringe is ingeniously used, and a detachable connection system is designed, and each unit is assembled into a closed container, which facilitates sample transportation;And the sample taking mode of pushing the micro sample out from the micro injection pipe 104 below the sample storage cavity 101 is adopted, which eliminates the technical defects of liquid residue in the traditional taking process, thereby improving the sample utilization rate;In addition, the bucket-shaped input port 103 is arranged above the sample storage cavity 101, which facilitates sample injection.

[0055] The above merely preferred embodiments of the present application are not used to limit the present application, any modification, equivalent replacement and improvement made within the spirit and principle of the present application should be included in the protection scope of the present application. The above is merely the preferred embodiment of the present application, it should be pointed out that, for the ordinary skilled in the art, without departing from the technical principle of the present application, a number of improvements and variations can be made, these improvements and variations should also be considered as the protection scope of the present application.

Claims

1. A micro-liquid sample storage and retrieval container, characterized by, The application relates to a sample storage unit (1) directly containing a liquid sample, a sample taking unit (2) arranged at the input end of the sample storage unit (1), and a discharge head protection unit (3) arranged at the output end of the sample storage unit (1). The sample storage unit (1) comprises a first column-shaped base (100), a sample storage cavity (101) vertically arranged in the first column-shaped base (100), a micro-discharge pipe (104) arranged at the output end of the sample storage cavity (101), an interface (113) arranged at the output end of the sample storage cavity (101), a first connecting thread (114) formed in the inner side wall of the interface (113), a first connecting ring (111) and a rubber sealing protrusion (112) arranged on the lower surface of the first column-shaped base (100), and a connecting thread arranged on the outer side wall of the first connecting ring (111); the connecting thread on the outer side wall of the first connecting ring (111) is used for connecting the discharge head protection unit (3), the rubber sealing protrusion (112) is in close contact with the discharge head protection unit (3) to realize the air-tight effect around the micro-discharge pipe (104), and the first connecting thread (114) is used for connecting the sample taking unit (2). The sample taking unit (2) comprises a piston discharge assembly (210) used for pressurizing the sample storage cavity (101) to push the sample downwards from the micro-discharge pipe (104), and a trigger prevention cover (220) arranged at the back of the piston discharge assembly (210) to place the piston and prevent the piston from being pushed. The inlet of the sample storage cavity (101) is a bucket-shaped input port (103).

2. A micro-liquid sample storage and retrieval vessel according to claim 1, wherein, The outlet of the sample storage cavity (101) is a tapered output port (102).

3. A micro-liquid sample storage and retrieval vessel according to claim 1, wherein, A sealing strip (115) is arranged between the contact surface of the sample storage unit (1) and the sample taking unit (2).

4. A micro-liquid sample storage and retrieval vessel according to claim 1, wherein, The piston discharge assembly (210) comprises a second column-shaped base (211), a piston sliding cavity (215) arranged on the second column-shaped base (211), a piston (216) and a push rod (217) sliding in the piston sliding cavity (215), a second connecting ring (213) arranged on the lower surface of the second column-shaped base (211), and a second connecting thread (214) arranged on the outer side of the second connecting ring (213) and threadedly connected with the first connecting thread (114).

5. A micro-liquid sample storage and retrieval vessel according to claim 1, wherein, A third connecting ring (218) is arranged on the upper surface of the second column-shaped base (211), and a third connecting thread (219) is arranged on the outer side of the third connecting ring (218); the third connecting thread (219) is used for connecting the trigger prevention cover (220). The trigger prevention cover (220) comprises a third column-shaped base (221), a fourth connecting thread (222) formed in the inner side wall of the third column-shaped base (221), and a non-slip surface (223) arranged on the outer side of the third column-shaped base (221); the fourth connecting thread (222) is threadedly fixedly connected with the third connecting thread (219). The discharge head protection unit (3) comprises 6. A micro-liquid sample storage and retrieval vessel according to claim 5, wherein, ​ ​ 7. A micro-liquid sample storage and retrieval vessel according to claim 6, wherein, ​ The fourth cylindrical base (300) is provided with a protection cavity (301) for inserting and protecting the micro injection tube (104), a connecting ring groove (302) and a sealing alignment ring groove (304) on the upper surface of the fourth cylindrical base (300), and a fifth connecting thread (303) on the inner side of the connecting ring groove (302); The fifth connecting thread (303) is connected with the outer side connecting thread of the first connecting ring (111), and the sealing alignment ring groove (304) is tightly connected with the rubber sealing protrusion (112).

8. A micro-liquid sample storage and retrieval vessel according to claim 7, wherein, The rotation directions of the first connecting thread (114) and the second connecting thread (214) are opposite to those of the third connecting thread (219) and the fourth connecting thread (222).

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