Cryopreservation tube rack for multi-tube cell resuscitation

Abstract

The utility model discloses a cryopreservation tube rack for multi-tube cell resuscitation, which relates to the technical field of cell resuscitation and particularly comprises a water bath kettle body, a cryopreservation tube rack and a connecting rod, the cryopreservation tube rack is placed in the water bath kettle body, the connecting rod is inserted into the top of the cryopreservation tube rack, the water bath kettle body further comprises a top cover, and the top cover is arranged on the top of the cryopreservation tube rack. A rotating bead is rotationally installed in the center of the top of the top cover, and the connecting rod penetrates through the top cover and the rotating bead. According to the utility model, through the arrangement of the outer diameters of the main rod and the handle on the connecting rod and the cooperation of the spring and the limiting pin, the connecting rod can penetrate through the rotating bead on the top cover, and then the connecting rod can be rotated back and forth and left and right to drive the test tubes on the cryopreservation tube rack to shake; the shaking amplitude of the connecting rod can be limited, and the situation that cells flow out or water submerges test tube openings to pollute the cells due to the fact that the cryopreservation tube frame is too inclined is avoided.

Description

Technical Field

[0001] This utility model relates to the field of cell resuscitation technology, specifically a cryopreservation rack for multi-tube cell resuscitation. Background Technology

[0002] Cell resuscitation, a biological term, refers to the process of thawing cells frozen in liquid nitrogen or at -80°C and then reculturing them to resume growth. The cryovials must be removed from liquid nitrogen and quickly placed in a 37°C water bath to thaw. After thawing, they should be quickly removed from the 37°C water bath to reduce cell death. When placing the cells in the water bath, the cryovials should be held with tweezers and gently shaken occasionally to ensure even heating and prevent water from entering the cryovials and contaminating the cells.

[0003] Existing cell resuscitation devices have simple structures and poor functionality. If cells in multiple cryopreservation tubes need to be resuscitated, the experimenter can only operate them one by one with hand tweezers, which takes too long and has low work efficiency. Utility Model Content

[0004] (a) Technical problems to be solved

[0005] To address the shortcomings of existing technologies, this invention provides a cryopreservation tube rack for multi-tube cell resuscitation, solving the problems mentioned in the background section.

[0006] (II) Technical Solution

[0007] To achieve the above objectives, this utility model is implemented through the following technical solution: it includes a water bath body, a cryopreservation tube rack, and a connecting rod. The cryopreservation tube rack is placed inside the water bath body, and the connecting rod is inserted into the top of the cryopreservation tube rack. The water bath body also includes a top cover, and a rotating ball is rotatably installed at the top center of the top cover. The connecting rod passes through the top cover and the rotating ball.

[0008] The cryopreservation tube rack includes a base plate, several positioning rings, an upper fixing plate, and two limiting plates. The positioning rings are fixedly installed on the top surface of the base plate, the upper fixing plate is fixedly installed on the upper side of the base plate, and the two limiting plates are slidably installed on the top of the upper fixing plate.

[0009] The connecting rod includes a main rod, a limiting ring, two limiting pins, a spring, a protrusion, and a handle. The spring is located on the inner side of the main rod, the two limiting pins are slidably mounted on the side surface of the main rod, the limiting ring is fixedly mounted on the outer side of the main rod, the protrusion is fixedly mounted on the bottom outer side of the main rod, and the handle is fixedly mounted on the top of the main rod.

[0010] Optionally, a through hole is provided at the center of the rotating bead, the inner diameter of the through hole being equal to the outer diameter of the main rod of the connecting rod, and the main rod of the connecting rod passing through the rotating bead.

[0011] Optionally, the limiting ring is hemispherical, and a placement groove is provided at the center of the hemispherical shape of the limiting ring. The inner diameter of the placement groove is equal to the outer diameter of the rotating bead, and the inner surface of the placement groove of the limiting ring is in contact with the outer surface of the rotating bead.

[0012] Optionally, the top of the upper fixing plate is provided with a rotating groove, which is composed of two opposing quarter-segmented grooves. The radius of the quarter-segmented groove is equal to the rotation radius of the protrusion on the connecting rod, and the protrusion on the connecting rod is rotatably connected to the rotating groove of the upper fixing plate.

[0013] Optionally, the top of the upper fixing plate has two limiting grooves, the inner width and length of the limiting grooves are equal to the width and length of the center side of the limiting plate, and the limiting plate is slidably installed on the top of the upper fixing plate.

[0014] Optionally, a sliding hole is provided on the side wall of the main rod. The inner diameter of the center of the sliding hole is larger than the inner diameters at both ends. The inner diameter of the center of the sliding hole is equal to the outer diameter of the spring. The spring is disposed inside the sliding hole.

[0015] Optionally, the limiting pin is a stepped frustum shape, and the outer diameter of one end of the limiting pin is equal to the inner diameter of both ends of the sliding hole. The limiting pin is slidably installed inside the sliding hole.

[0016] This invention provides a cryopreservation tube rack for multi-tube cell resuscitation, which has the following beneficial effects:

[0017] 1. This cryopreservation tube rack for multi-tube cell resuscitation allows multiple test tubes to be stored on the rack through the cooperation of the base plate, positioning ring and upper fixing plate. Then, the connection rod cooperates with the upper fixing plate and limiting plate to facilitate the removal of the cryopreservation tube rack from liquid nitrogen, avoiding frostbite caused by manual removal by staff using tweezers.

[0018] 2. This cryopreservation tube rack for multi-tube cell resuscitation, through the setting of the outer diameter of the main rod and handle on the connecting rod, and the cooperation of the spring and the limiting pin, allows the connecting rod to pass through the rotating bead on the top cover. Then, by rotating the connecting rod back and forth and left and right, the test tubes on the cryopreservation tube rack can be shaken. At the same time, the setting of the limiting ring can limit the shaking amplitude of the connecting rod, and prevent the cryopreservation tube rack from tilting too much, causing cells to flow out or the water surface to submerge the test tube opening and contaminate the cells. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0020] Figure 2 This is a schematic diagram of the internal structure of the water bath of this utility model;

[0021] Figure 3 This is a schematic diagram of the connection structure between the connecting rod and the cryopreservation tube rack of this utility model;

[0022] Figure 4 This is a schematic diagram of the internal structure of the upper fixing plate of this utility model;

[0023] Figure 5 This is a schematic diagram of the limit pin installation structure of this utility model;

[0024] Figure 6 This is a schematic diagram of the shaking limit structure of the cryopreservation tube rack of this utility model.

[0025] In the diagram: 1. Water bath body; 101. Top cover; 102. Rotating ball; 2. Cryopreservation tube rack; 201. Base plate; 202. Positioning ring; 203. Upper fixing plate; 204. Limiting plate; 3. Connecting rod; 301. Main rod; 302. Limiting ring; 303. Limiting pin; 304. Spring; 305. Protrusion; 306. Handle. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0027] Example

[0028] Please see Figures 1 to 6 The present invention provides a technical solution including a water bath body 1, a cryopreservation tube rack 2, and a connecting rod 3. The cryopreservation tube rack 2 is placed inside the water bath body 1, and the connecting rod 3 is inserted into the top of the cryopreservation tube rack 2. The water bath body 1 also includes a top cover 101. The water bath body 1 adopts the Changzhou Guohua HH-4 digital display constant temperature circulating water bath to achieve a constant temperature heating effect for cells. A rotating bead 102 is rotatably installed at the top center of the top of the top cover 101, and the connecting rod 3 passes through the top cover 101 and the rotating bead 102.

[0029] The cryopreservation tube rack 2 includes a base plate 201, several positioning rings 202, an upper fixing plate 203, and two limiting plates 204. The positioning rings 202 are fixedly installed on the top surface of the base plate 201, the upper fixing plate 203 is fixedly installed on the upper side of the base plate 201, and the two limiting plates 204 are slidably installed on the top of the upper fixing plate 203.

[0030] The connecting rod 3 includes a main rod 301, a limiting ring 302, two limiting pins 303, a spring 304, a protrusion 305, and a handle 306. The spring 304 is located on the inner side of the main rod 301. The two limiting pins 303 are slidably mounted on the side surface of the main rod 301. The limiting ring 302 is fixedly mounted on the outer side of the main rod 301. The protrusion 305 is fixedly mounted on the bottom outer side of the main rod 301. The handle 306 is fixedly mounted on the top of the main rod 301.

[0031] Specifically, the base plate 201, positioning ring 202, and upper fixing plate 203 on the cryopreservation tube rack 2 work together to store multiple test tubes. The connecting rod 3, in conjunction with the upper fixing plate 203 and limiting plate 204, facilitates the removal of the cryopreservation tube rack 2 from liquid nitrogen, avoiding frostbite caused by manual removal with tweezers. The outer diameter of the main rod 301 and handle 306 on the connecting rod 3, along with the spring 304 and limiting pin 303, allows the connecting rod 3 to pass through the rotating bead 102 on the top cover 101. Rotating the connecting rod 3 forward, backward, left, and right can cause the test tubes on the cryopreservation tube rack 2 to shake. The limiting ring 302 limits the shaking amplitude of the connecting rod 3, preventing the cryopreservation tube rack 2 from tilting too much, causing cells to flow out or the water surface to submerge the test tube openings and contaminate the cells.

[0032] Please refer to Figure 2 and Figure 3 A through hole is provided at the center of the rotating bead 102. The inner diameter of the through hole is equal to the outer diameter of the main rod 301 of the connecting rod 3. The main rod 301 of the connecting rod 3 passes through the rotating bead 102. The maximum outer diameter of the handle 306 is smaller than the inner diameter of the through hole.

[0033] The limiting ring 302 is hemispherical, and a mounting groove is provided at the center of the hemispherical shape. The inner diameter of the mounting groove is equal to the outer diameter of the rotating bead 102, and the inner surface of the mounting groove of the limiting ring 302 is in contact with the outer surface of the rotating bead 102.

[0034] Specifically, by passing the connecting rod 3 through the rotating bead 102 and utilizing the mounting method of the rotating bead 102 on the top cover 101, the connecting rod 3 can rotate back and forth and left and right with the center of the rotating bead 102 as the origin.

[0035] Please see Figures 3 to 4 The top of the upper fixing plate 203 is provided with a rotating groove, which is composed of two opposing quarter-shaped fan grooves. The radius of the fan groove is equal to the rotation radius of the protrusion 305 on the connecting rod 3. The protrusion 305 of the connecting rod 3 is rotatably connected to the rotating groove of the upper fixing plate 203.

[0036] The top of the upper fixed plate 203 has two limiting grooves. The inner width and length of the limiting grooves are equal to the width and length of the center side of the limiting plate 204, respectively. The limiting plate 204 is slidably installed on the top of the upper fixed plate 203. The limiting plate 204 has an I-shaped structure. Magnetic blocks are provided on the bottom surface of the inner side of the limiting groove and the top surface of the limiting plate 204 where it falls into the fan-shaped groove. The limiting plate 204 is made of a low-temperature resistant metal material that can magnetically attract the magnetic blocks.

[0037] Specifically, the connection between the connecting rod 3 and the cryopreservation tube rack 2 is achieved by rotating the connecting rod 3 after it is inserted into the rotating groove, so that the cryopreservation tube rack 2 can be taken out of the liquid nitrogen through the connecting rod 3.

[0038] Please see Figure 5 A sliding hole is provided on the side wall of the main rod 301. The inner diameter of the center of the sliding hole is larger than the inner diameters at both ends. The inner diameter of the center of the sliding hole is equal to the outer diameter of the spring 304. The spring 304 is located inside the sliding hole.

[0039] The limiting pin 303 is a stepped frustum shape. The outer diameter of the minor diameter end of the limiting pin 303 is equal to the inner diameter of both ends of the sliding hole. The limiting pin 303 is slidably installed inside the sliding hole.

[0040] Specifically, through the cooperation of spring 304 and sliding hole, the limiting pin 303 can be pressed into the main rod 301 when the main rod 301 passes through the rotating ball 102, so as to facilitate the connection between the connecting rod 3 and the rotating ball 102.

[0041] In use, by inserting the connecting rod 3 into the upper fixing plate 203 of the cryopreservation tube rack 2, and inserting the main rod 301 and the bottom protrusion 305 of the connecting rod 3 into the rotating groove of the upper fixing plate 203, rotating the main rod 301 ninety degrees, the cryopreservation tube rack 2 can be removed entirely from the liquid nitrogen, avoiding frostbite caused by manual removal with tweezers. When thawing cells, remove the top cover 101, allowing the top of the main rod 301 to pass through the rotating bead 102. The top cover 101 moves downwards on the main rod 301. Pressing the limiting pin 303 will then... The positioning pin 303 is pressed into the main rod 301, which does not hinder the movement of the top cover 101 on the main rod 301. This allows the bottom of the rotating bead 102 on the top cover 101 to contact the bottom of the inner side of the limiting ring 302. Then, rotating the connecting rod 3 can cause the test tubes on the cryopreservation tube rack 2 to shake. Utilizing the effect of the limiting ring 302, after the connecting rod 3 rotates to a certain extent, the limiting ring 302 contacts the bottom surface of the top cover 101, limiting the shaking amplitude of the connecting rod 3 and preventing the cryopreservation tube rack 2 from tilting too much, causing cells to flow out or the water surface to submerge the test tube opening and contaminate the cells.

[0042] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A cryotube rack for multi-tube cell recovery, comprising a water bath body (1), a cryotube rack (2) and a connecting rod (3), characterized in that: The freezing tube rack (2) is placed in the water bath body (1), the connecting rod (3) is inserted in the top of the freezing tube rack (2), the water bath body (1) further includes a top cover (101), the top center of the top cover (101) is rotatably installed with a rotating ball (102), the connecting rod (3) penetrates the top cover (101) and the rotating ball (102); The freezing tube rack (2) includes a bottom plate (201), a plurality of positioning rings (202), an upper fixed plate (203) and two limiting plates (204), a plurality of the positioning rings (202) are fixedly installed on the top surface of the bottom plate (201), the upper fixed plate (203) is fixedly installed on the upper side of the bottom plate (201), and the two limiting plates (204) are slidably installed on the top of the upper fixed plate (203). The connecting rod (3) includes a main rod (301), a limiting ring (302), two limiting pins (303), a spring (304), a protruding block (305) and a handle (306), the spring (304) is arranged on the inner side of the main rod (301), the two limiting pins (303) are slidably installed on the side surface of the main rod (301), the limiting ring (302) is fixedly installed on the outer side of the main rod (301), the protruding block (305) is fixedly installed on the bottom outer side of the main rod (301), and the handle (306) is fixedly installed on the top of the main rod (301).

2. A cryo-preservation rack for multi-tube cell recovery according to claim 1, wherein: The center position of the rotating ball (102) is provided with a through hole, the inner diameter of the through hole is equal to the outer diameter of the main rod (301) of the connecting rod (3), and the main rod (301) of the connecting rod (3) penetrates the rotating ball (102).

3. A cryo-preservation rack for multi-tube cell recovery according to claim 1, wherein: The limiting ring (302) is semispherical, a placing groove is formed in the semispherical center of the limiting ring (302), the inner diameter of the placing groove is equal to the outer diameter of the rotating ball (102), and the inner surface of the placing groove of the limiting ring (302) is in contact with the outer surface of the rotating ball (102).

4. The cryotube rack for multi-tube cell recovery of claim 1, wherein: A rotating groove is formed in the top of the upper fixed plate (203), the rotating groove is composed of two opposite quarter fan-shaped grooves, the radius of the fan-shaped groove is equal to the rotating radius of the protruding block (305) of the connecting rod (3), and the protruding block (305) of the connecting rod (3) is rotatably connected in the rotating groove of the upper fixed plate (203).

5. The cryo-preservation rack for multi-tube cell recovery of claim 1, wherein: Two limiting grooves are formed in the top of the upper fixed plate (203), the inner side width and length of the limiting groove are equal to the width and length of the center side of the limiting plate (204) respectively, and the limiting plate (204) is slidably installed on the top of the upper fixed plate (203).

6. A cryo-preservation rack for multi-tube cell recovery according to claim 1, wherein: A sliding hole is formed in the side wall of the main rod (301), the center inner diameter of the sliding hole is greater than the inner diameters of two ends, the center inner diameter of the sliding hole is equal to the outer diameter of the spring (304), and the spring (304) is arranged in the sliding hole.

7. The cryotube rack for multi-tube cell recovery of claim 1, wherein: The limiting pin (303) is in a stepped circular cone shape, the outer diameter of the small-diameter end of the limiting pin (303) is equal to the inner diameters of two ends of the sliding hole, and the limiting pin (303) is slidably installed in the sliding hole.

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