Immune cell long-term storage activity maintaining device

Abstract

The utility model provides an immune cell long-term storage activity maintaining device, which relates to the technical field of cell storage and comprises a storage box, a storage cavity is arranged in the storage box, an arc cavity is arranged on the surface of the storage box, and four liquid nitrogen tank bodies are arranged in the arc cavity at equal intervals. According to the liquid nitrogen tank, the plurality of liquid nitrogen tank bodies are mounted in the arc cavity, so that the effect that a low-temperature environment in the storage cavity cannot be maintained due to the damage of one liquid nitrogen tank body can be prevented, and the effect of preventing the damage of cells due to the damage of the liquid nitrogen tank bodies can be achieved; when the liquid nitrogen in one liquid nitrogen tank body is exhausted, the other liquid nitrogen tank body can operate again, the effect that the low-temperature environment is not stable in the liquid nitrogen supplementing process due to the fact that the liquid nitrogen in the liquid nitrogen tank body is exhausted can be prevented, and therefore the effect of improving the stability of the low-temperature environment can be achieved.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell storage technical field especially relates to a long -term storage activity maintaining device of immune cell. BACKGROUND

[0002] In recent years, immune cell therapy has made revolutionary progress in the clinical field and become a new hope for fighting various difficult diseases. For example, CAR-T cell therapy, through gene modification of the patient's own T cells, can accurately recognize and kill tumor cells, and has shown significant efficacy in the treatment of hematological tumors such as leukemia and lymphoma, and some patients have achieved long-term remission or even cure. In addition, NK cell therapy, TCR-T cell therapy and other immune cell therapies have also shown good prospects in clinical trials and are gradually applied to the treatment of solid tumors, autoimmune diseases and other diseases. However, the successful implementation of these therapies depends on high-quality, stable immune cells. After collecting immune cells from the patient's body, a series of complex processes such as in vitro culture, modification and quality detection are often required, which takes a long time, which requires a reliable storage device to properly preserve cell activity before treatment to ensure the effectiveness and timeliness of cell therapy, thereby creating a strong demand for long-term storage activity maintaining devices for immune cells.

[0003] The existing immune cell long-term storage usually relies on a single liquid nitrogen tank to maintain a low-temperature environment, which has great hidden dangers. The liquid nitrogen tank, as the core refrigeration equipment, contains complex refrigeration, insulation and pressure control systems inside. Once a key component such as the refrigeration compressor or the vacuum insulation layer fails, it will directly disrupt the low-temperature balance inside the tank. Due to the lack of a standby liquid nitrogen tank to take over in time, the storage temperature will rise at an extremely fast rate. According to relevant experimental data, when the liquid nitrogen tank refrigeration system fails completely, the storage chamber temperature may rise from -196℃ to above -80℃ within 1-2 hours, and under such temperature fluctuations, the ice crystals in the immune cells rapidly melt and recrystallize, causing irreversible damage to the cell membrane and organelles, and a large number of immune cells will be inactivated within a short period of time, which is undoubtedly devastating to scientific research projects that have invested a lot of manpower and resources and patients waiting for cell therapy. At the same time, the immune cell storage device of the liquid nitrogen tank has obvious deficiencies in liquid nitrogen level monitoring. The common monitoring method is manual regular inspection, which determines the remaining amount of liquid nitrogen by observing the liquid level gauge of the liquid nitrogen tank. This method is inefficient and relies on manual operation, and if the operator is negligent or the inspection period is too long, it will be difficult to discover that the liquid nitrogen is about to run out in time, and even if the lack of liquid nitrogen is discovered in time, the liquid nitrogen replenishment process also takes a certain amount of time, which can easily affect the cell storage activity. UTILITY MODEL CONTENTS

[0004] The purpose of this invention is to solve the problem in the existing technology that when a single liquid nitrogen tank is damaged or the liquid nitrogen is depleted, it cannot maintain the low temperature environment in time, and proposes a device for long-term storage and activity maintenance of immune cells.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: a device for long-term storage and maintenance of the activity of immune cells, comprising a storage box, wherein a storage cavity is formed inside the storage box, an arc cavity is formed on the surface of the storage box, four liquid nitrogen tank bodies are installed at equal intervals inside the arc cavity, a U-shaped tube is fixedly connected inside the storage cavity, an arc tube is fixedly connected between the U-shaped tubes, an air outlet is fixedly installed at equal intervals on the surface of the U-shaped tube, a connecting pipe is fixedly connected at equal intervals on the surface of the arc tube, the other end of the connecting pipe penetrates the inner wall of the storage cavity and is fixedly connected to the surface of the liquid nitrogen tank body, a fixed cover is fixedly connected through and near the center of the top of the storage box, and a plurality of test tube holes are formed on the bottom inner wall surface of the fixed cover.

[0006] Preferably, the storage box is fixedly connected to support legs at equal intervals at the bottom and near the edge, and the support legs are all trapezoidal in shape.

[0007] Preferably, control valves are fixedly installed on the surface of each connecting pipe.

[0008] Preferably, a liquid nitrogen level display is fixedly installed on the surface of the liquid nitrogen tank body.

[0009] Preferably, an arc cover is connected to the inside of the arc cavity and near the outer side by a pin, and the arc cover is transparent.

[0010] Preferably, an L-shaped handle is fixedly connected to the outer arc wall of the arc cover near one end.

[0011] Preferably, a temperature sensor is fixedly installed on the bottom inner wall of the storage cavity near the edge.

[0012] Preferably, a control panel is fixedly installed on the outer arc wall surface of the storage box, and the control panel is electrically connected to the temperature sensor.

[0013] Preferably, the top of each liquid nitrogen tank body is fixed and connected to a liquid nitrogen replenishment pipe, and the top of each liquid nitrogen replenishment pipe penetrates the inner wall of the top of the arc cavity and is fixedly connected to it.

[0014] Preferably, a round cover is fitted and installed on the top of the fixed cover.

[0015] Compared with the prior art, the advantages and positive effects of this utility model are as follows:

[0016] 1. The utility model discloses a plurality of liquid nitrogen tank bodies are installed in the arc cavity, can prevent the effect that one liquid nitrogen tank body is damaged and leads to the low temperature environment inside the storage cavity can not be maintained, thereby can reach the effect that prevent the cell damage caused by the liquid nitrogen tank body damage.

[0017] 2. The utility model discloses a plurality of liquid nitrogen tank bodies are installed in the arc cavity, when one liquid nitrogen tank body inside liquid nitrogen is exhausted, can operate again through another liquid nitrogen tank body, can play the effect that prevents the low temperature environment instability in the process of supplementing liquid nitrogen caused by the liquid nitrogen tank body inside liquid nitrogen exhaustion, thereby can reach the effect that improve the stability of low temperature environment maintenance. BRIEF DESCRIPTION OF DRAWINGS

[0018] Figure 1 The utility model proposes a whole structure perspective drawing of immune cell long -term storage activity maintaining device;

[0019] Figure 2 The utility model proposes a partial structure perspective drawing of immune cell long -term storage activity maintaining device;

[0020] Figure 3 The utility model proposes a whole structure vertical section view of immune cell long -term storage activity maintaining device;

[0021] Figure 4 The utility model proposes a whole structure horizontal section view of immune cell long -term storage activity maintaining device;

[0022] Figure 5 The utility model proposes a U -shaped tube structure perspective drawing of immune cell long -term storage activity maintaining device.

[0023] Legend explanation: 1, storage box, 2, support leg, 3, storage cavity, 4, arc cavity, 5, liquid nitrogen tank body, 6, U -shaped tube, 7, gas outlet, 8, arc pipe, 9, connecting pipe, 10, control valve, 11, liquid nitrogen amount display, 12, arc cover, 13, L type handle, 14, temperature sensor, 15, control panel, 16, liquid nitrogen supplement pipe, 17, fixed cover, 18, round cover, 19, test tube hole. DETAILED DESCRIPTION

[0024] In order to more clearly understand the above -mentioned purpose, feature and advantage of the utility model, the utility model is further explained below with the help of drawings and examples. It needs to be explained that the embodiment of the application and the feature in the embodiment can be combined mutually in the case of not conflicting.

[0025] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Therefore, the present invention is not limited to the specific embodiments disclosed in the following specification.

[0026] Example 1, such as Figures 1-5 As shown, this utility model provides a device for long-term storage and maintenance of the activity of immune cells, including a storage box 1. The storage box 1 has a storage cavity 3 inside, and an arc cavity 4 is formed on the surface of the storage box 1. Four liquid nitrogen tank bodies 5 are installed at equal intervals inside the arc cavity 4. U-shaped tubes 6 are fixedly connected inside the storage cavity 3. Arc tubes 8 are fixedly connected between the U-shaped tubes 6. Air outlets 7 are fixedly installed at equal intervals on the surface of the U-shaped tubes 6. Connecting tubes 9 are fixedly connected at equal intervals on the surface of the arc tubes 8. The other end of the connecting tube 9 penetrates the inner wall of the storage cavity 3 and is fixedly connected to the surface of the liquid nitrogen tank body 5. A fixing cover 17 is fixedly connected through and near the center of the top of the storage box 1. Multiple test tube holes 19 are formed on the bottom inner wall surface of the fixing cover 17.

[0027] The overall effect of embodiment 1 is as follows: a storage cavity 3 is provided inside the storage box 1, and an arc cavity 4 is provided on the surface of the storage box 1. Four liquid nitrogen tank bodies 5 are installed at equal intervals inside the arc cavity 4, which can allow multiple liquid nitrogen tank bodies 5 to fill the storage cavity 3 with liquid nitrogen gas. A U-shaped tube 6 is fixedly connected inside the storage cavity 3. Arc tubes 8 are fixed and connected between the U-shaped tubes 6. Gas outlets 7 are fixedly installed at equal intervals on the surface of the U-shaped tubes 6. Connecting pipes 9 are fixedly connected at equal intervals on the surface of the arc tubes 8. The other end of the connecting pipe 9 penetrates the inner wall of the storage cavity 3 and is fixed and connected to the surface of the liquid nitrogen tank body 5, which can allow the liquid nitrogen tank body 5 to enter through the connecting pipe 9 and the U-shaped tube 6 and exit through the gas outlet 7. A fixed cover 17 is fixedly connected through the top of the storage box 1 and near the center. Multiple test tube holes 19 are provided on the bottom inner wall surface of the fixed cover 17, which can allow test tubes to be placed inside the test tube holes 19.

[0028] Example 2, as Figures 1-5As shown, the bottom of the storage box 1 is fixedly connected with support legs 2 at intervals near the edge, and the shape of the support legs 2 is trapezoidal; the surface of the connecting pipe 9 is fixedly installed with a control valve 10; the surface of the liquid nitrogen tank body 5 is fixedly installed with a liquid nitrogen amount display 11; the inside of the arc cavity 4 is fixedly connected with an arc cover 12 near the outer side pin shaft, and the arc cover 12 is transparent; the outer arc wall of the arc cover 12 is fixedly connected with an L-shaped handle 13 near one end; the bottom inner wall of the storage cavity 3 is fixedly installed with a temperature sensor 14 near the edge; the outer arc wall surface of the storage box 1 is fixedly installed with a control panel 15, and the control panel 15 is electrically connected with the temperature sensor 14; the top of the liquid nitrogen tank body 5 is fixedly connected with a liquid nitrogen supplement pipe 16, and the top of the liquid nitrogen supplement pipe 16 penetrates through the top inner wall of the arc cavity 4 and is fixedly connected therewith; the top of the fixed cover 17 is sleeved and installed with a round cover 18.

[0029] The effect achieved by the whole embodiment 2 is that the bottom of the storage box 1 is fixedly connected with support legs 2 at intervals near the edge, and the shape of the support legs 2 is trapezoidal, which can support the bottom of the storage box 1; the surface of the connecting pipe 9 is fixedly installed with a control valve 10, which can control the opening and closing of the connecting pipe 9; the surface of the liquid nitrogen tank body 5 is fixedly installed with a liquid nitrogen amount display 11, which can display the amount inside the liquid nitrogen tank body 5; the inside of the arc cavity 4 is fixedly connected with an arc cover 12 near the outer side pin shaft, and the arc cover 12 is transparent, which can protect the liquid nitrogen tank body 5; the outer arc wall of the arc cover 12 is fixedly connected with an L-shaped handle 13 near one end, which can facilitate pulling the arc cover 12; the bottom inner wall of the storage cavity 3 is fixedly installed with a temperature sensor 14 near the edge, which can sense the temperature inside the storage cavity 3 and transmit the temperature to the control panel 15; the outer arc wall surface of the storage box 1 is fixedly installed with a control panel 15, and the control panel 15 is electrically connected with the temperature sensor 14, which can control the device; the top of the liquid nitrogen tank body 5 is fixedly connected with a liquid nitrogen supplement pipe 16, and the top of the liquid nitrogen supplement pipe 16 penetrates through the top inner wall of the arc cavity 4 and is fixedly connected therewith, which can supplement the liquid nitrogen of the liquid nitrogen tank body 5; the top of the fixed cover 17 is sleeved and installed with a round cover 18, which can cover the fixed cover 17.

[0030] Working principle: by opening the round cover 18, the test tube containing immune cells can be placed into the test tube hole 19, then cover the round cover 18, control one of the control valves 10 through the control panel 15, the liquid nitrogen in the liquid nitrogen tank body 5 enters the arc tube 8 through the connecting pipe 9, the liquid nitrogen in the arc tube 8 enters the U-shaped tube 6 from both ends, the liquid nitrogen in the U-shaped tube 6 enters the storage cavity 3 through the gas outlet 7, the storage cavity 3 is cooled, when the liquid nitrogen in the liquid nitrogen tank body 5 is exhausted, another control valve 10 is opened to supply liquid nitrogen from another liquid nitrogen tank body 5, the exhausted liquid nitrogen tank body 5 is supplemented with liquid nitrogen through the liquid nitrogen supplementing pipe 16, the low-temperature environment is prevented from being unstable during the liquid nitrogen supplementing process, and the low-temperature environment of the storage cavity 3 is prevented from being maintained due to damage of the liquid nitrogen tank body 5, so that the cell damage caused by the damage of the liquid nitrogen tank body 5 is prevented.

[0031] The wiring diagram of the liquid nitrogen tank body 5, the control valve 10, the liquid nitrogen amount display 11, the temperature sensor 14 and the control panel 15 in the utility model is the common knowledge in the field, and the working principle is the known technology, and the type is selected according to the actual use, so the control mode and the wiring arrangement of the liquid nitrogen tank body 5, the control valve 10, the liquid nitrogen amount display 11, the temperature sensor 14 and the control panel 15 are not explained in detail.

[0032] The above is only the preferred embodiment of the utility model, and is not limited to other forms, and any skilled person in the art can change or modify the above disclosed technical content into equivalent embodiments applied to other fields, but any simple modification, equivalent change and modification of the above embodiments without departing from the technical scheme content of the utility model, according to the technical essence of the utility model, still belongs to the protection scope of the utility model technical scheme.

Claims

1. An immune cell long-term storage activity maintenance device comprising a storage tank (1), characterized in that: The inside of the storage box (1) is provided with a storage cavity (3), and the surface of the storage box (1) is provided with an arc cavity (4), four liquid nitrogen tank bodies (5) are installed in the arc cavity (4) at equal intervals, a U-shaped tube (6) is fixedly connected in the storage cavity (3), the U-shaped tubes (6) are fixedly and communicatively connected with an arc tube (8), gas outlets (7) are fixedly and installed on the surface of the U-shaped tubes (6) at equal intervals, connecting pipes (9) are fixedly and communicatively connected on the surface of the arc tube (8) at equal intervals, one end of the connecting pipe (9) penetrates the inner wall of the storage cavity (3) and is fixedly and communicatively connected with the surface of the liquid nitrogen tank body (5), a fixed cover (17) is fixedly and connected on the top of the storage box (1) and close to the center, a plurality of test tube holes (19) are formed in the bottom inner wall surface of the fixed cover (17).

2. The long-term storage active maintenance device for immune cells according to claim 1, wherein: The bottom of the storage box (1) is fixedly connected with support legs (2) at equal intervals close to the edges.

3. The long-term storage active maintenance device for immune cells according to claim 1, wherein: The surface of the connecting pipe (9) is fixedly installed with a control valve (10).

4. The long-term storage active maintenance device for immune cells according to claim 1, wherein: The surface of the liquid nitrogen tank body (5) is fixedly installed with a liquid nitrogen amount display (11).

5. The long-term storage active maintenance device for immune cells according to claim 1, wherein: The inside of the arc cavity (4) is fixedly connected with an arc cover (12) close to the outer side pin shaft.

6. The long-term storage active maintenance device for immune cells according to claim 5, wherein: The outer arc wall of the arc cover (12) is fixedly connected with an L-shaped handle (13) close to one end.

7. The long-term storage active maintenance device for immune cells according to claim 1, wherein: The bottom inner wall of the storage cavity (3) is fixedly installed with a temperature sensor (14) close to the edge.

8. The long-term storage active maintenance device for immune cells according to claim 7, wherein: The outer arc wall surface of the storage box (1) is fixedly installed with a control panel (15), and the control panel (15) is electrically connected with the temperature sensor (14).

9. The long-term storage active maintenance device for immune cells of claim 1, wherein: The top of the liquid nitrogen tank body (5) is fixedly and communicatively connected with a liquid nitrogen supplement pipe (16), and the top of the liquid nitrogen supplement pipe (16) penetrates the top inner wall of the arc cavity (4) and is fixedly connected therewith.

10. The long-term storage active maintenance device for immune cells of claim 1, wherein: The top of the fixed cover (17) is sleeved and installed with a round cover (18).

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