Aerobic and anaerobic biological strain mobile incubator for heavy oil sewage treatment

Abstract

The utility model discloses a movable aerobic and anaerobic biological strain incubator for heavy oil sewage treatment. The movable aerobic and anaerobic biological strain incubator comprises a main body culture tank, an air compressor, a stirring device, a cover tank and a heat exchanger, the temperature control area of the main body culture tank is located in the cover tank, a sterile air inlet is formed in the main body culture tank, and an exhaust connector of the air compressor is connected with the sterile air inlet. The integrated design is achieved, the movable function is achieved, microbial agents can be conveniently added at different places, the cost of strain culture and adding is greatly reduced, and the treatment efficiency is improved; by starting the stirring device and the air compressor at the same time, the movable incubator can effectively culture aerobic strains, and under the condition that only the stirring device is started, anaerobic strains can be cultured, so that one incubator can culture two different types of strains at the same time, and the application range is expanded.

Description

TECHNICAL FIELD

[0001] The utility model relates to thickened oil sewage treatment technical field especially, relates to a kind of aerobic and anaerobic biological species mobile incubator for thickened oil sewage treatment. BACKGROUND

[0002] Thickened oil sewage becomes the processing problem of oil industry due to its unique high temperature, high oil content, high emulsification degree, high chemical oxygen demand (COD), high suspended solids (SS) concentration, high silicon content and high salinity etc. characteristics. Traditional physical and chemical treatment means show its limitations when dealing with such sewage. In contrast, biological enhancement technology aims to improve the efficiency of wastewater treatment by regularly adding microorganisms and nutrients with specific degradation capacity.

[0003] However, the harmful ingredients in thickened oil sewage have inhibitory effect on the growth of microorganisms, which directly affects the treatment effect. In addition, the currently available strains have many problems, such as long domestication cycle, poor adaptability, low activity and difficulty in preservation, etc. These problems lead to the existing culture equipment complicated operation and high energy consumption. In order to solve these problems, it is urgent to develop more efficient and simple operation culture equipment to optimize the culture conditions of microorganisms, so as to improve the treatment effect of thickened oil sewage. SUMMARY

[0004] To solve the technical problem of the existing culture equipment complicated operation and high energy consumption, the utility model provides a kind of aerobic and anaerobic biological species mobile incubator for thickened oil sewage treatment. To have a basic understanding of some aspects of the disclosed embodiments, a brief summary is given below. This part is not a general review, nor is it intended to determine the key / important elements or delineate the protection scope of these embodiments. Its only purpose is to present some concepts in simple form as a prelude to the detailed description that follows.

[0005] The utility model adopts the following technical solutions:

[0006] Provide a kind of aerobic and anaerobic biological species mobile incubator for thickened oil sewage treatment, it include: main body culture jar, air compressor, cover jar and heat exchanger;

[0007] The temperature control area of the main body culture jar is located in the cover jar, and the outer wall circulating cavity is formed between the cover jar and the main body culture jar, the medium discharge port is arranged on the cover jar and connected with the outer wall circulating cavity, the sterile air inlet is arranged on the main body culture jar, and the exhaust port of the air compressor is connected with the sterile air inlet through the first gas pipeline;

[0008] The refrigerant inlet of the heat exchanger is connected with the air compressor exhaust interface through a second gas pipeline, the refrigerant outlet of the heat exchanger is connected with the sterile air inlet, and the heat medium inlet of the heat exchanger is connected with the medium discharge port.

[0009] Further, the aerobic and anaerobic biological bacteria mobile culture device for thickened oil sewage treatment further comprises a bacteria culture heater and a sterilization heater; the bacteria culture heater and the sterilization heater are arranged on the cover tank, the front end of the bacteria culture heater extends into the main body culture tank, and the front end of the sterilization heater extends into the outer wall circulating cavity and is located at the bottom of the main body culture tank.

[0010] Further, the aerobic and anaerobic biological bacteria mobile culture device for thickened oil sewage treatment further comprises an electrical control box, a mobile frame body and mobile wheels; the main body culture tank, the electrical control box, the air compressor and the heat exchanger are arranged on the mobile frame body, and the mobile wheels are arranged at the bottom of the mobile frame body.

[0011] Further, the aerobic and anaerobic biological bacteria mobile culture device for thickened oil sewage treatment further comprises a stirring device arranged on the main body culture tank; the stirring device comprises a motor, a stirring shaft body and stirring blades arranged in the main body culture tank; the motor is arranged at the top of the main body culture tank, the stirring blades are arranged on the stirring shaft body, and the stirring shaft body is connected with the power output end of the motor.

[0012] Further, the aerobic and anaerobic biological bacteria mobile culture device for thickened oil sewage treatment further comprises a feeding port, a medium input port, a discharge port and a sampling valve; the feeding port is arranged at the top of the main body culture tank, the medium input port is arranged on the cover tank, the discharge port is arranged at the bottom of the main body culture tank, and the sampling valve is arranged on the discharge port.

[0013] Further, the aerobic and anaerobic biological bacteria mobile culture device for thickened oil sewage treatment further comprises an exhaust valve, a safety valve and a pressure gauge; the exhaust valve, the safety valve and the pressure gauge are arranged at the top of the main body culture tank.

[0014] Further, the aerobic and anaerobic biological bacteria mobile culture device for thickened oil sewage treatment further comprises a sight glass, a temperature electrode, a dissolved oxygen electrode and a pH electrode arranged on the main body culture tank.

[0015] Further, the aerobic and anaerobic biological bacteria mobile culture device for thick oil sewage treatment further comprises a first control valve and a second control valve, the first control valve is arranged on the first gas pipeline, and the second control valve is arranged on the second gas pipeline.

[0016] Further, the electric control box is internally provided with a controller, the pressure gauge, the temperature electrode, the dissolved oxygen electrode, the pH electrode, the sensors of the bacteria culture heater and the sterilization heater are connected with a data input interface of the controller, and the exhaust valve, the first control valve, the second control valve, the motor, the air compressor, the bacteria culture heater and the sterilization heater are connected with a control output interface of the controller.

[0017] The utility model brings the beneficial effect that:

[0018] 1. simple structure, convenient operation,

[0019] 2. integrated design, has mobile function, can conveniently add the bacteria agent in different places, greatly reduces the cost of bacteria culture and addition, improves the processing efficiency,

[0020] 3. by simultaneously opening the stirring device and the air compressor, the mobile culture device can effectively culture aerobic bacteria, and in the case that only the stirring device is opened, anaerobic bacteria can be cultured, so that one culture device can culture two different types of bacteria at the same time, and the application range is expanded,

[0021] 4. the temperature of air is adjusted by using temperature control medium, so that the culture requirement of aerobic bacteria is better adapted, not only the efficiency of bacteria culture is improved, but also the energy consumption is significantly reduced. BRIEF DESCRIPTION OF DRAWINGS

[0022] In order to more clearly illustrate the technical scheme in the embodiments of the utility model or the prior art, the following will briefly introduce the drawings needed to be used in the embodiment or the prior art description, and obviously, the drawings in the following description are only some embodiments of the utility model, and for those skilled in the art, other drawings can be obtained according to these drawings without creative labor.

[0023] Figure 1 It is a structure schematic view of the aerobic and anaerobic biological bacteria mobile culture device for thick oil sewage treatment of the utility model. DETAILED DESCRIPTION

[0024] The embodiments of the present application will be described in detail below with reference to the drawings. It should be noted that the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of the present application.

[0025] As shown in the drawings, in some illustrative embodiments, an aerobic and anaerobic biological species mobile culture device for thick oil sewage treatment is provided, comprising: a main culture tank 100, an air compressor 200, a cover tank 300, a heat exchanger 400, a species culture heater 500, a sterilization heater 600, an electrical control box 700, a mobile frame body 800, and a stirring device 900. Figure 1 The upper half of the main culture tank 100 is a reaction zone, and the lower half is a temperature control zone. The main culture tank 100 is a stainless steel cylinder, which ensures its corrosion resistance and stability when treating high-temperature and highly corrosive thick oil sewage. The cylindrical shape can uniformly distribute the internal pressure to improve the safety performance of the culture device.

[0026] The temperature control zone of the main culture tank 100 is located in the cover tank 300, i.e., the cover tank 300 is arranged around the lower half of the main culture tank 100, so that an outer wall circulating cavity 310 for heat exchange is formed between the cover tank 300 and the main culture tank 100. The cover tank 300 is provided with a medium discharge port 320 and a medium input port 330 which are in communication with the outer wall circulating cavity 310. When the temperature control zone needs to be heated, the heat medium is pumped into the outer wall circulating cavity 310 through the medium input port 330. The heat medium flows in the outer wall circulating cavity 310 and transfers heat to the inside of the tank body through the outer wall of the tank body, thereby increasing the temperature in the tank body. When the temperature control zone needs to be cooled, the cold medium is introduced into the outer wall circulating cavity 310 through the medium input port 330, so that the temperature in the tank body is reduced. The medium after heat exchange is discharged to the outside of the outer wall circulating cavity 310 through the medium discharge port 320.

[0027] The main culture tank 100 is provided with a sterile air inlet 110, which is specifically arranged on the inner side wall of the upper half of the reaction zone of the main culture tank 100. The sterile air inlet 110 comprises an air filter, and the air filter is a two-stage air filter. The first-stage filter is used to intercept larger particles and most microorganisms, and the second-stage filter is used to further purify the air to ensure sterility. The sterile air inlet 110 uses the air filter to intercept bacteria entering the tank body to create a sterile environment, which improves the efficiency of biological enrichment culture and reduces the risk of pollution.

[0028]

[0029] ​The air compressor 200 is connected to the sterile air inlet 110 through the first air pipeline 210, and the compressed air has been compressed and preliminarily filtered before entering the culture tank. When the air reaches the sterile air inlet 110 through the first air pipeline 210, it will be further purified by two-stage filters, finally providing a sterile oxygen environment for the microorganisms in the main culture tank 100, promoting their growth and metabolism.

[0030] The refrigerant inlet of the heat exchanger 400 is connected to the air outlet of the air compressor 200 through the second air pipeline 220, the refrigerant outlet of the heat exchanger 400 is connected to the sterile air inlet 110, and the heat medium inlet of the heat exchanger 400 is connected to the medium discharge port 320.

[0031] When the main culture tank 100 needs to be kept warm, the outer wall circulating cavity 310 is filled with hot medium. These hot medium provides heat for the main culture tank 100 while generating excess heat. The excess heat is guided to the heat medium inlet of the heat exchanger 400 through the medium discharge port 320, and the heat medium transfers the heat to the compressed air entering through the second air pipeline 220. The compressed air absorbs the heat released by the heat medium in the heat exchanger 400, its temperature rises, then enters the sterile air inlet 110 through the refrigerant outlet, and finally enters the main culture tank 100, providing warm and sterile air for aerobic bacteria. By recycling and utilizing the excess heat generated during the warming process, energy waste is reduced. The heat exchanger 400 can adjust the temperature of the air entering the tank, better meeting the culture needs of aerobic bacteria. By recycling heat, the additional heating requirement is reduced, thereby reducing the overall energy consumption.

[0032] The bacteria culture heater 500 and the sterilization heater 600 are both arranged on the cover tank 300, the front end of the bacteria culture heater 500 extends into the main culture tank 100, and the front end of the sterilization heater 600 extends into the outer wall circulating cavity 310 and is located at the bottom of the main culture tank 100. The front end refers to the end of the heater that can provide heat. When the sterilization heater 600 is started, a sterile environment is created to remove bacteria. When the bacteria culture heater 500 is started, it directly provides heat to the inside of the main culture tank 100, avoiding the adverse effects of temperature fluctuations on the growth of bacteria, effectively ensuring the growth temperature of microorganisms, and improving the quality of bacteria. After the bacteria culture is completed, the water cooling is shortened, and the use efficiency of the culture device is improved.

[0033] The stirring device 900 is arranged on the main culture tank 100. Specifically, the stirring device 900 comprises a motor 910, a stirring shaft body 920, and a stirring paddle 930 arranged inside the main culture tank 100. The motor 910 is arranged on the top of the main culture tank 100, the stirring paddle 930 is arranged on the stirring shaft body 920, the top end of the stirring shaft body 920 is connected with the power output end of the motor 910, and the bottom end of the stirring shaft body 920 extends into the main culture tank 100.

[0034] The stirring device 900 ensures the homogeneity of the liquid in the process of strain culture, ensures the uniform distribution of nutrients and strains in the culture solution, and promotes the growth and metabolism of the strains. When only anaerobic strains need to be cultured, the stirring device 900 is only turned on. The stirring can provide sufficient liquid flow to maintain the growth environment of the strains without introducing additional oxygen. When aerobic strains need to be cultured, the stirring device 900 and the air compressor 200 are turned on at the same time. The air compressor 200 provides air into the tank through the sterile air inlet 110, and the stirring device 900 ensures the full mixing of air and culture solution, thereby improving the oxygen transfer efficiency. The culture device can culture two different types of strains at the same time, thereby expanding the application range.

[0035] The main culture tank 100, the electrical control box 700, the air compressor 200, and the heat exchanger 400 are arranged on the movable frame body 800, and the bottom of the movable frame body 800 is provided with movable wheels 810. The integrated design has the function of moving, and the addition of bacterial agents can be conveniently carried out at different places, thereby greatly reducing the cost of strain culture and addition, and improving the processing efficiency.

[0036] The feeding port 120 is arranged on the top of the main culture tank 100, which is convenient for adding culture medium, strains or other additives from the top. The discharge port 130 is arranged on the bottom of the main culture tank 100, and the sampling valve 140 is arranged on the discharge port 130. The discharge port 130 is a channel for discharging the culture solution, and the sampling valve 140 is used to take samples from the main culture tank 100 to analyze the pH value, nutrient concentration, strain growth condition and other parameters of the culture solution.

[0037] The exhaust valve 150, the safety valve 160, and the pressure gauge 170 are arranged on the top of the main culture tank 100. The exhaust valve 150 is used to release the gas generated in the tank to maintain the pressure balance in the tank and prevent overpressure. For example, by exhausting, the oxygen concentration in the tank can be controlled, and at the same time, the exhaust valve 150 can also help to remove harmful gases generated during the culture process. When the pressure in the tank exceeds the preset safety value, the safety valve 160 will automatically open to release the excess pressure to prevent the tank from exploding or being damaged due to high pressure. The pressure gauge 170 is used to monitor the pressure change in the tank in real time.

[0038] The sight glass 180, temperature electrode 1, dissolved oxygen electrode 2 and pH electrode 3 are arranged on the main culture tank 100. The sight glass 180 is a transparent observation window arranged on the side of the main culture tank 100, which is convenient for the operator to directly observe the state of the culture solution and the growth of the organisms in the tank. The temperature electrode 1 is used to measure the temperature of the liquid in the culture tank and transmit the data to the electrical control box 700 so as to adjust the temperature environment required for the growth of the bacteria. The dissolved oxygen electrode 2 is used to monitor the dissolved oxygen concentration in the culture solution. By monitoring the dissolved oxygen level in real time, the aeration amount and stirring speed can be adjusted to ensure that the microorganisms always grow in the best oxygen condition. The pH electrode 3 is used to measure the pH value of the culture solution, so that the operator can timely adjust the acidity and alkalinity to maintain the optimal pH environment for the growth of the microorganisms.

[0039] The first control valve 230 is arranged on the first gas supply pipeline 210, and the second control valve 240 is arranged on the second gas supply pipeline 220. When the aerobic bacteria are cultured, if the main culture tank 100 is kept warm, the outer wall circulating cavity 310 has a heat medium, at this time, the first control valve 230 is closed and the second control valve 240 is opened, and the residual heat of the heat medium heats the sterile air. If the outer wall circulating cavity 310 does not have heating conditions, the first control valve 230 is opened and the second control valve 240 is closed. When the anaerobic bacteria are cultured, the first control valve 230 and the second control valve 240 are both closed.

[0040] The controller is arranged in the electrical control box 700. The pressure gauge 170, temperature electrode 1, dissolved oxygen electrode 2, pH electrode 3, and the sensors of the bacteria culture heater 500 and the sterilization heater 600 are connected with the data input interface of the controller. The exhaust valve 150, the first control valve 230, the second control valve 240, the motor 910, the air compressor 200, the bacteria culture heater 500 and the sterilization heater 600 are connected with the control output interface of the controller.

[0041] When the culture device of the embodiment works, first, the main culture tank 100 needs to be completely sterilized, the discharge port 130 and the sampling valve 140 are closed, water is added to the middle line of the sight glass 180, the sterilization switch is started, when the temperature reaches 121℃, it is maintained for more than 30 minutes, and the exhaust valve 150 is always slightly open. After the air sterilization is completed, the valve of the discharge port 130 at the bottom of the main culture tank 100 is opened, and the water is discharged to enter the normal production.

[0042] The funnel is inserted into the feeding port 120, and the prepared culture medium concentrate is directly added or pumped into the main culture tank 100 by the water pump, then sewage is added to adjust the liquid to the standard amount, the feeding port 120 is tightly screwed, and the sterilization heater 600 is turned on, so that the culture medium can be sterilized.

[0043] Turn on the sterilization switch, preset the sterilization temperature to 121°C, and start the stirring device 900 at low speed to mix the materials in the main culture tank 100. When the pressure reaches 0.05 MPa, slightly open the exhaust valve 150 for 3 minutes to remove the cold air. When the temperature reaches the set temperature, adjust the exhaust valve 150 to be slightly open again to maintain the temperature and start timing for 30 minutes (different timing for different culture media). During the timing process, release the material once in the front, middle, and back, each time for 3-5 minutes, and a total of 3-5 liters for three times. Turn off the sterilization switch when the time is up, immediately pass the medium inlet 330 to the outer wall circulating cavity 310 to cool the water, and then the water in the outer wall circulating cavity 310 is discharged through the medium outlet 320 for reuse. When the temperature drops to 80-85 degrees and the pressure in the tank body is lower than 0.05 MPa, turn on the motor 910 to speed up the cooling speed, keep the pressure in the main culture tank 100 at 0.05 MPa, and until the temperature of the culture medium in the main culture tank 100 drops to the inoculation temperature.

[0044] Gradually open the exhaust valve 150, and when the pressure in the main culture tank 100 drops to near 0, quickly close the exhaust valve 150 and ignite the fire ring, unscrew the loading cover, remove the cotton plug above the flame, quickly pour the strain into the tank, tighten the loading cover, adjust the pressure in the main culture tank 100 to about 0.02 MPa or positive pressure, start the culture switch, and enter the strain culture stage.

[0045] The temperature of the biological strain culture is generally 28-37 degrees, the pressure in the main culture tank 100 is about 0.02 MPa or positive pressure, the culture time is determined according to the process requirements, and is generally 24-72 hours. Turn on the strain culture heater 500 during culture. If aerobic bacteria are needed, the stirring device 900 and air compressor 200 need to be turned on at the same time; if anaerobic bacteria are needed, only the stirring device 900 needs to be turned on.

[0046] After inoculation and during the culture process, take samples every 4-8 hours for inspection, which can be done through the sampling valve 140. Use a microscope for microscopic examination to observe the culture conditions. During the operation process, the temperature, dissolved oxygen, and pH in the main culture tank 100 can be observed through the sight glass 180, temperature electrode 1, dissolved oxygen electrode 2, and pH electrode.

[0047] When the concentration of the bacteria reaches the process requirement, the fermentation is ended and the discharge is carried out. The discharge is to discharge the bacteria liquid through the discharge port 130 by using the tank pressure, and according to the concentration of the bacteria liquid, the pressure in the main culture tank 100 can be controlled at 0.05-0.1 MPa. After the discharge is ended, the inside of the main culture tank 100 and the pipeline valve should be immediately washed by water, and the auxiliary air compressor 200 is started to supply air to the main culture tank 100 and stir, so as to flush the bacteria liquid in the pipeline. If the tank is not used temporarily, the main culture tank 100 is subjected to air sterilization, and the water in the main culture tank 100, the outer wall circulation cavity 310 and the pipeline is emptied.

[0048] The operation is simple, the cost is low, the strain can be cultivated efficiently and flexibly, the microorganism capable of degrading pollutants can be cultivated in a large amount according to the characteristics of the sewage, and the microorganism can be added to the biological treatment tank every day and used in biochemical treatment, so that the start-up time is shortened. The aerobic and anaerobic bacteria can be cultivated at the same time, the reproduction and biofilm formation speed of the aerobic and anaerobic bacteria in the biochemical system are improved, and the degradation of the organic pollutants in the heavy oil sewage is accelerated. The problems of long domestication period, poor applicability, low activity, difficult preservation and the like of the high-temperature and high-salt bacteria for degrading the heavy oil sewage are solved.

[0049] The above merely describes the specific implementation of the present application, but the protection scope of the present application is not limited to this. Any person skilled in the art can easily think of the changes or replacements within the technical range disclosed by the present application, which should be covered in the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. An aerobic and anaerobic biological bacteria species mobile culture device for thick oil sewage treatment, characterized in that, The utility model relates to a kind of fermentation device, including: Main culture tank, air compressor, cover tank and heat exchanger; The temperature control area of the main culture tank is located in the cover tank, and the outer wall circulation cavity is formed between the cover tank and the main culture tank, the cover tank is provided with medium discharge port connected with the outer wall circulation cavity, the main culture tank is provided with sterile air inlet, and the air outlet of the air compressor is connected with the sterile air inlet through first gas pipeline; The refrigerant inlet of the heat exchanger is connected with the air outlet of the air compressor through second gas pipeline, the refrigerant outlet of the heat exchanger is connected with the sterile air inlet, and the heat medium inlet of the heat exchanger is connected with the medium discharge port.

2. The aerobic and anaerobic biological bacteria mobile culture device for thick oil sewage treatment according to claim 1, characterized in that, It also includes: strain culture heater and sterilization heater; The strain culture heater and the sterilization heater are both arranged on the cover tank, the front end of the strain culture heater extends into the main culture tank, and the front end of the sterilization heater extends into the outer wall circulation cavity and is located at the bottom of the main culture tank.

3. The aerobic and anaerobic biological bacteria mobile culture device for thick oil sewage treatment according to claim 2, characterized in that, It also includes: Electric control box, mobile frame and mobile wheel;The main culture tank, the electric control box, the air compressor and the heat exchanger are arranged on the mobile frame, and the mobile wheel is arranged at the bottom of the mobile frame.

4. The aerobic and anaerobic biological bacteria mobile culture device for thick oil sewage treatment according to claim 3, characterized in that, It also includes: stirring device, which is arranged on the main culture tank;The stirring device includes motor, stirring shaft body and stirring paddle in the main culture tank, the motor is arranged at the top of the main culture tank, the stirring paddle is arranged on the stirring shaft body, and the stirring shaft body is connected with the power output end of the motor.

5. The aerobic and anaerobic biological bacteria mobile culture device for thick oil sewage treatment according to claim 4, characterized in that, It also includes: Feed inlet, medium inlet, discharge outlet and sampling valve;The feed inlet is arranged at the top of the main culture tank, the medium inlet is arranged on the cover tank, the discharge outlet is arranged at the bottom of the main culture tank, and the sampling valve is arranged on the discharge outlet.

6. The aerobic and anaerobic biological bacteria mobile culture device for thick oil sewage treatment according to claim 5, characterized in that, It also includes: Exhaust valve, safety valve and pressure gauge, the exhaust valve, the safety valve and the pressure gauge are arranged at the top of the main culture tank.

7. The aerobic and anaerobic biological bacteria mobile culture device for thick oil sewage treatment according to claim 6, characterized in that, It also includes: View mirror, temperature electrode, dissolved oxygen electrode and pH electrode arranged on the main culture tank.

8. The aerobic and anaerobic biological bacteria mobile culture device for thick oil sewage treatment according to claim 7, characterized in that, It also includes: First control valve and second control valve;The first control valve is arranged on the first gas pipeline, and the second control valve is arranged on the second gas pipeline.

9. The aerobic and anaerobic biological bacteria mobile culture device for thick oil sewage treatment according to claim 8, characterized in that, Controller is arranged in the electric control box;The pressure gauge, the temperature electrode, the dissolved oxygen electrode, the pH electrode and the sensors of strain culture heater and sterilization heater are connected with the data input interface of the controller;The exhaust valve, the first control valve, the second control valve, the motor, the air compressor, the strain culture heater and the sterilization heater are connected with the control output interface of the controller.

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