Sample lysis incubation equipment

Abstract

The utility model provides sample lysis incubation equipment. The sample lysis incubation equipment comprises an object placing device, a detection device and a control device, wherein the object placing device is used for placing a sample plate to be treated and reagents and consumables required in a lysis incubation process; the centrifugal device is used for carrying out centrifugal treatment on the sample plate to be treated; the liquid separating device is used for automatically separating and injecting a reagent into the centrifuged sample plate; the film sealing device is used for packaging a sample plate filled with a sample and a reagent; the transfer device is used for automatically transferring the sample plate; the sample treatment device comprises a temperature control mechanism and / or an oscillation mechanism, the temperature control mechanism is used for performing temperature control treatment on the sample plate filled with the sample and the reagent, and the oscillation mechanism is used for uniformly shaking the sample plate filled with the sample and the reagent. At least two of the storage device, the centrifugal device, the liquid separation device, the film sealing device and the sample treatment device are located within the transfer range of the transfer device, so that the efficiency is improved, and the error rate and the working intensity of experimenters are reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of detection instrument, concretely relates to a sample lysing incubation equipment. BACKGROUND

[0002] The existing CRC, Meta and HP lysing incubation process is divided into several procedures and is operated in a backward mode, mainly by manual operation. The liquid separation and film sealing processes are time-consuming and labor-consuming, and the manual operation has many steps and a large workload, and the manual operation is prone to errors, resulting in the technical problems of time and labor consumption and low efficiency in the whole process of fecal sample lysing incubation.

[0003] Therefore, in order to meet the market demand, it is urgent to improve the existing preparation mode to save labor cost and improve the efficiency of product preparation. UTILITY MODEL CONTENT

[0004] The utility model mainly solves the technical problem, provide a kind of sample lysing incubation equipment, to improve efficiency, reduce error rate and reduce the working strength of experimenter.

[0005] The scheme for achieving the technical purpose of the utility model is a sample lysing incubation equipment, comprising:

[0006] The storage device is used to place the sample plate to be processed and the reagents and consumables required in the lysing incubation process.

[0007] The centrifugal device is used to centrifugally process the sample plate to be processed.

[0008] The liquid separation device is used to automatically inject reagents into the sample plate after centrifugal processing.

[0009] The film sealing device is used to automatically package the sample plate containing samples and reagents after liquid separation.

[0010] The sample processing device comprises at least one of a temperature control mechanism and a shaking mechanism, the temperature control mechanism is used to control the temperature of the sample plate containing samples and reagents, and the shaking mechanism is used to shake the sample plate containing samples and reagents.

[0011] And the transfer device, at least two of the storage device, the centrifugal device, the liquid separation device, the film sealing device and the sample processing device are located within the transfer range of the transfer device, and the transfer device is used to automatically transfer the sample plate.

[0012] In an alternative embodiment, the transfer device comprises a track and a robot, the robot is installed on the track and can move along the track, and the storage device, the centrifugal device, the liquid separation device, the film sealing device and the sample processing device are arranged on at least one side of the track.

[0013] In an alternative embodiment, the track is a straight track, and the sample processing device comprises a temperature control mechanism and a shaking mechanism; the temperature control mechanism, the shaking mechanism, the dispensing device and the centrifugal device are arranged on one side of the track, and the sealing device is arranged on the other side of the track.

[0014] In an alternative embodiment, the temperature control mechanism and the shaking mechanism are both multiple, and the multiple temperature control mechanisms and the multiple shaking mechanisms are arranged in a row in the moving direction of the manipulator along the track.

[0015] In an alternative embodiment, the dispensing device and the centrifugal device are arranged in a row in the moving direction of the manipulator along the track, and the temperature control mechanism and the shaking mechanism are located between the track and the dispensing device.

[0016] In an alternative embodiment, the storage device comprises a storage mechanism and an interactive rack, the storage mechanism is used for placing consumables and reagent plates; the interactive rack has multiple placement parts, the placement parts are used for positioning sample plates to be processed, and the storage mechanism and the interactive rack are located within the transfer range of the transfer device.

[0017] In an alternative embodiment, the storage mechanism and the sealing device are arranged on the same side of the track, and the interactive rack is arranged at one end of the track and is spaced apart from the track in the moving direction of the manipulator along the track.

[0018] In an alternative embodiment, the storage device further comprises a temporary storage rack, the temporary storage rack is used for receiving reagent plates and consumables of the storage mechanism, the temporary storage rack is adjacent to the dispensing device and is arranged on the same side of the track, and the centrifugal device, the dispensing device and the temporary storage rack are arranged in a row in sequence in the moving direction of the manipulator along the track.

[0019] In an alternative embodiment, the fecal sample lysis incubation equipment further comprises a film tearing device, the film tearing device is used for tearing film processing on the sample plate or the well plate covered with film; the film tearing device and the sealing device are arranged adjacent to each other and are arranged on the same side of the track.

[0020] The placement device, the centrifugal device, the dispensing device, the sealing device, the film tearing device and the sample processing device are all located within the transfer range of the manipulator, and the manipulator realizes automatic transfer of the sample plate between the devices and mechanisms.

[0021] In an alternative embodiment, the sample lysis incubation equipment further comprises a base, and the centrifugal device, the dispensing device, the sealing device and the sample processing device are all mounted on the base.

[0022] The sample lysis incubation equipment further comprises a receiving mechanism, the receiving mechanism is detachably mounted on the base, the base is provided with a through hole, and the waste reagent plate and the consumable can be put into the receiving mechanism through the through hole.

[0023] According to the sample lysis incubation apparatus of the above embodiment, the placement device is used to place the sample plate to be processed, as well as the reagents and consumables required for the lysis incubation process. Transfer operations during the fecal sample lysis incubation process may include moving the sample plate from the placement device to the centrifugation device, from the centrifugation device to the dispensing device, from the dispensing device to the sealing device, from the dispensing device to the temperature control device, and from the sealing device to the centrifugation device, etc. If at least two of the placement device, centrifugation device, dispensing device, sealing device, and temperature control mechanism are within the transfer range of the transfer device, then the sample plate, reagent plate, or consumables can be automatically transferred between at least two of the placement device, centrifugation device, dispensing device, sealing device, and sample processing device via the transfer device. In other words, the transfer steps in the process include the transfer of sample plates, reagent plates, and consumables. At least some of the transfer operations can be automated by the transport device. The centrifugation device, dispensing device, sealing device, and sample processing device are also automated equipment, which helps to reduce the time spent on fecal sample lysis and incubation and reduce the error rate. Compared with related technologies, it can also reduce the amount of manual work to a certain extent and improve the efficiency of fecal sample lysis and incubation. Attached Figure Description

[0024] Figure 1 This is an overall schematic diagram of the sample lysis and incubation equipment provided in some embodiments of the present invention.

[0025] Figure 2 for Figure 1 Top view.

[0026] Figure 3 for Figure 1 A schematic diagram showing the arrangement of the transfer device, the interaction rack, and the sample processing device.

[0027] Reference numerals: 10-Placement device; 11-Storage mechanism; 12-Interaction rack; 13-Temporary storage rack; 14-Limiting corner block; 20-Centrifugation device; 30-Dispensing device; 40-Sealing device; 50-Sample processing device; 51-Temperature control mechanism; 52-Shaking mechanism; 60-Transfer device; 61-Railway; 62-Robot arm; 70-Tearing device; 81-Receiving mechanism; 82-Guide channel; 83-Bar scanner; 84-Detection sensor; 85-Transfer rack; 86-Calibration protrusion; 87-Protective plate; 90-Base; 91-Through hole. Detailed Implementation

[0028] The utility model makes further detailed description through specific implementation mode combining with the drawings. Similar elements in different implementation modes adopt the relevant similar element mark. In the following implementation mode, many details are described in order to make the application be better understood. However, the person skilled in the art can easily realize that part of the features can be omitted in different cases, or can be replaced by other elements, materials, methods. In some cases, some operations related to the application are not shown or described in the specification, in order to avoid the core part of the application from being overwhelmed by too much description, and for the person skilled in the art, it is not necessary to describe these related operations in detail, they can complete the understanding of the related operation according to the description in the specification and the general technical knowledge in the art.

[0029] In addition, the features, operations or characteristics described in the specification can be combined in any appropriate manner to form various embodiments. At the same time, the steps or actions in the method description can also be sequentially replaced or adjusted in a manner obvious to those skilled in the art. Therefore, the various sequences in the specification and drawings are only for the purpose of clearly describing a certain embodiment, and do not mean the necessary sequence, unless otherwise stated that a certain sequence must be followed.

[0030] The serial numbers of components in this paper, such as "first", "second", etc., are only used to distinguish the described objects, and have no any sequence or technical meaning. The "connection" and "coupling" in the application include direct and indirect connection (coupling) unless otherwise specified.

[0031] The existing CRC, Meta and HP cracking incubation sub-process operation mode is relatively backward, mainly using manual and semi-automatic equipment to assist. The liquid separation and film sealing consume time and effort, the whole process time is long, and the manual work is easy to make mistakes, so it is necessary to upgrade it to an unattended device to save labor and improve efficiency.

[0032] To solve the above technical problems, the utility model provides a sample cracking incubation equipment, please refer to Figures 1-3The sample lysis incubation device includes a placing device 10, a centrifugal device 20, a liquid separation device 30, a film sealing device 40, a sample processing device 50, and a transfer device 60. The placing device 10 is used to place a sample plate to be processed and reagents and consumables required in the lysis incubation process. The centrifugal device 20 is used to centrifugally process the sample plate to be processed. The liquid separation device 30 is used to automatically inject reagents into the sample plate after centrifugal processing. The film sealing device 40 is used to automatically package the sample plate containing samples and reagents after liquid separation. The sample processing device 50 includes at least one of a temperature control mechanism 51 for temperature control processing of the sample plate containing samples and reagents and a shaking mechanism 52 for uniform shaking processing of the sample plate containing samples and reagents. At least two of the placing device 10, the centrifugal device 20, the liquid separation device 30, the film sealing device 40, and the sample processing device 50 are within the transfer range of the transfer device 60, and the transfer device 60 is used to automatically transfer the sample plate.

[0033] According to the sample lysis incubation device in the above embodiment, the placing device 10 is used to place a sample plate to be processed and reagents and consumables required in the lysis incubation process. The transfer operation in the fecal sample lysis incubation process can include moving the sample plate from the placing device 10 to the centrifugal device 20, transferring from the centrifugal device 20 to the liquid separation device 30, transferring from the liquid separation device 30 to the film sealing device 40, transferring from the liquid separation device 30 to the temperature control device, and transferring from the film sealing device 40 to the centrifugal device 20, and the like. At least two of the placing device 10, the centrifugal device 20, the liquid separation device 30, the film sealing device 40, and the sample processing device 50 are within the transfer range of the transfer device 60, so that the transfer device 60 can automatically transfer the sample plate or the reagent plate or the consumable between at least two of the placing device 10, the centrifugal device 20, the liquid separation device 30, the film sealing device 40, and the sample processing device 50. That is, the transfer in the process steps includes the transfer of the sample plate, the transfer of the reagent plate, and the transfer of the consumable, and at least part of the transfer operation can be automatically realized by the transfer device 60. The centrifugal device 20, the liquid separation device 30, the film sealing device 40, and the sample processing device 50 are also automatic devices, which is beneficial to reduce the time consumption of the fecal sample lysis incubation, reduce the error rate, and also reduce the workload of manual operation to a certain extent compared with the related art, and is beneficial to improve the working efficiency of the fecal sample lysis incubation.

[0034] It should be noted that the above-mentioned "the sample processing device 50 comprises at least one of the temperature control mechanism 51 and the oscillation mechanism 52" means that there are three options. In some embodiments, the sample processing device 50 can only have the temperature control mechanism 51, which is suitable for the lysis incubation of the fecal sample which needs to be bathed, i.e. enzyme reaction. In some embodiments, the sample processing device 50 can only have the oscillation mechanism 52, which is suitable for the lysis incubation process of the fecal sample which needs to be mixed by oscillation. In some other embodiments, please refer to Figure 1 and Figure 3 , the sample processing device 50 can simultaneously comprise the temperature control mechanism 51 and the oscillation mechanism 52, which can be suitable for the lysis incubation of the fecal sample which needs to be bathed and / or mixed by oscillation, and can effectively expand the application range of the sample lysis incubation equipment.

[0035] In addition, the function of the temperature control mechanism 51 can also be adaptively adjusted and selected according to actual needs. For example, in some embodiments, the temperature control mechanism 51 can only be used to heat the sample and stabilize the temperature of the sample in the required range. In some other embodiments, in addition to the function of heating the sample plate, the temperature control mechanism 51 can also have the function of accelerating the cooling of the sample plate to room temperature, so as to effectively shorten the time of the whole fecal sample lysis incubation process.

[0036] In some embodiments, please refer to Figure 1 , Figure 2 and Figure 3 , the transfer device 60 comprises a track 61 and a mechanical hand 62, the mechanical hand 62 is installed on the track 61 and can move along the track 61, and the placing device 10, the centrifugal device 20, the liquid separation device 30, the film sealing device 40 and the sample processing device 50 are arranged on at least one side of the track 61. On the one hand, by arranging the track 61 and enabling the mechanical hand 62 to move along the track 61, the transfer range of the mechanical hand 62 can be expanded. On the other hand, by arranging the placing device 10, the centrifugal device 20, the liquid separation device 30, the film sealing device 40 and the sample processing device 50 around the track 61, the volume of the sample lysis incubation equipment can be controlled, and the user can be facilitated to use.

[0037] The number of the track 61 and the mechanical hand 62 can be adaptively adjusted according to needs, and the utility model does not make specific limitation. In some embodiments, please refer to Figure 1 , the track 61 and the mechanical hand 62 can be used in pairs, i.e. one track 61 is provided with one mechanical hand 62. In some other embodiments, at least two mechanical hands 62 can be arranged on one track 61.

[0038] In some embodiments, please refer to Figure 1 and Figure 3The mechanical arm 62 can include a six-axis mechanical arm and an electric gripper, and a clamping block can be arranged on the electric gripper to increase the clamping area and stabilize clamping of the sample plate, the reagent plate and the consumables.

[0039] In some embodiments, the transfer device 60 further includes a driving assembly for driving the mechanical arm 62 to move along the track 61, and the driving assembly can be any feasible technical solution, such as a belt drive, a lead screw drive, etc., which is not limited in the utility model. In some embodiments, the driving assembly can include a driving motor, a speed reducer and matching rack and pinion, and the pinion is driven by the driving motor and the speed reducer, so that the mechanical arm 62 can move along the rack and the track 61.

[0040] In some embodiments, please refer to Figure 1 and Figure 3 The sample processing device 50 includes a temperature control mechanism 51 and a shock mechanism 52, which can be suitable for the lysis incubation scene of the fecal sample with the need for temperature bath and / or the need for shock mixing, and can effectively expand the application range of the sample lysis incubation equipment.

[0041] In some embodiments, please refer to Figures 1-3 The track 61 is a straight track 61, so that the mechanical arm 62 can have a larger transfer range. The temperature control mechanism 51, the shock mechanism 52, the liquid separation device 30 and the centrifugal device 20 are arranged on one side of the track 61, and the film sealing device 40 is arranged on the other side of the track 61. On the basis of the straight track 61 of the track 61, by reasonably distributing the layout of each mechanism and each device, the mechanical arm 62 can automatically transfer the sample plate to the next automatic process.

[0042] In some embodiments, please refer to Figure 3 The temperature control mechanism 51 and the shock mechanism 52 are both multiple, so that the sample processing device 50 can process multiple sample plates at the same time, which is beneficial to improve the work efficiency. In the moving direction of the mechanical arm 62 along the track 61, the multiple temperature control mechanisms 51 and the multiple shock mechanisms 52 are arranged in a row, so that the space layout is more rationalized, and the overall volume of the sample lysis incubation equipment can be effectively controlled.

[0043] In some embodiments, please refer to Figures 1-3 The liquid separation device 30 and the centrifugal device 20 are arranged in a row in the moving direction of the mechanical arm 62 along the track 61, and the temperature control mechanism 51 and the shock mechanism 52 are located between the track 61 and the liquid separation device 30. The temperature control mechanism 51 and the shock mechanism 52 are both small-volume structures, which will not block the operation of the mechanical arm 62 at the centrifugal device 20 and the liquid separation device 30, and can reasonably utilize the space between the track 61 and the centrifugal device 20 and the space between the track 61 and the liquid separation device 30.

[0044] In some embodiments, please refer to Figures 1-3The storage device includes a storage mechanism 11 and an interactive rack 12. The storage mechanism 11 is used to place consumables and reagent plates. The interactive rack 12 has a plurality of placement portions used to position sample plates to be processed for manual feeding by an operator. The storage mechanism 11 and the interactive rack 12 are located within the transfer range of the transfer device 60. The transfer device 60 can automatically transfer the sample plates after feeding to the centrifugal device 20, and can also automatically transfer the reagent plates and consumables in the storage mechanism 11 to the dispensing device 30. After completing the final processing, the transfer device 60 can also automatically transfer the sample plates to be unloaded to the interactive rack 12, waiting for manual unloading by the operator for subsequent detection processing.

[0045] In some embodiments, in order to reduce the error rate of the automated process, the interactive rack 12 can be provided with a feeding area and an unloading area.

[0046] The specific structure of the storage mechanism 11 is not limited in the embodiment, and in some embodiments, the storage mechanism 11 can be a fixed rack structure, and the taking out and placing in of the well plate and consumables during the process are directly realized by the transfer device 60. In some embodiments, the storage mechanism 11 can also have the function of automatic unloading, and can adjust the required consumables or reagent plates and wait for the gripper 62 to pick them up.

[0047] In some embodiments, please refer to Figures 1-3 The storage mechanism 11 and the sealing film device 40 are located on the same side of the track 61, and the interactive rack 12 is located at one end of the track 61 and is spaced apart from the track 61 in the moving direction of the gripper 62 along the track 61, so as to make full use of the space at both ends of the track 61, which is conducive to controlling the overall occupied space.

[0048] In some embodiments, please refer to Figures 1-3 The placement portion can be a placement space defined by four limiting corner blocks 14, and one sample plate is positioned by the four limiting corner blocks 14. Since the four corners of the sample plate are only limited, two opposite sides of the sample plate can be exposed, so as to facilitate the gripper 62 to pick up the sample plate.

[0049] In some embodiments, in order to improve the automation degree of the equipment and reduce the error probability, the sample lysis and incubation equipment further includes a plurality of detection sensors 84, one detection sensor 84 is arranged in one placement portion, and is used to detect whether a sample plate is placed in the placement portion.

[0050] In some embodiments, please refer to Figures 1-3 The interactive rack 12 is provided with a protective plate 87 for protecting the detection sensor 84, which can effectively protect the detection sensor 84 and prolong the service life of the detection sensor 84.

[0051] In some embodiments, please refer to Figures 1-3, in order to improve work efficiency, the storage device further comprises a temporary storage rack 13 for receiving the reagent plate and consumables of the storage mechanism 11, which can be transferred manually or by the transfer device 60. The temporary storage rack 13 is adjacent to the dispensing device 30 and is located on the same side of the track 61. In the moving direction of the manipulator 62 along the track 61, the centrifugal device 20, the dispensing device 30 and the temporary storage rack 13 are arranged in a row in sequence. The temporary storage rack 13 is arranged close to the dispensing device 30, which is convenient for the dispensing device 30 to use the required reagents and consumables, and is also conducive to the overall volume of the sample lysis and incubation equipment.

[0052] In some embodiments, please refer to Figures 1-3 The temporary storage rack 13 has a plurality of plate positioning structures for positioning the reagent plate and the consumable box. Similarly, the plate positioning structure can include four limiting corner blocks 14, which are arranged at the four corners of the reagent plate or the consumable box, and then two opposite sides of the reagent plate or the consumable box can be exposed to facilitate the manipulator 62 to clamp and transfer.

[0053] In some embodiments, please refer to Figure 1 and Figure 3 The fecal sample lysis and incubation equipment further comprises a film tearing device 70 for automatically tearing the film of the sample plate or the well plate covered with film. By using an automatic device instead of manual film tearing, the efficiency can be improved and the error rate can be reduced.

[0054] As a feasible arrangement, please refer to Figure 1 and Figure 3 The film tearing device 70 and the film sealing device 40 are arranged adjacent to each other on the same side of the track 61. In order to achieve better results and improve the degree of automation, the placement device 10, the centrifugal device 20, the dispensing device 30, the film sealing device 40, the film tearing device 70 and the sample processing device 50 are all located within the transfer range of the manipulator 62. The manipulator 62 automatically transfers the sample plate between the devices and mechanisms, which can greatly improve the efficiency, reduce the amount of manual work, and at the same time ensure the yield.

[0055] In some embodiments, please refer to Figures 1-3 The sample lysis and incubation equipment further comprises a base 90, and the centrifugal device 20, the dispensing device 30, the film sealing device 40 and the sample processing device 50 are all mounted on the base 90.

[0056] In some embodiments, the interaction rack 12, the temporary storage rack 13, the centrifugal device 20, the dispensing device 30, the film sealing device 40, the film tearing device 70 and the sample processing device 50 are all arranged on the base 90. Please refer to Figures 1-3 The base 90 comprises a plurality of base tables which are fixed by splicing assembly, which is convenient for assembly and subsequent use and maintenance.

[0057] In some embodiments, referring to Figures 1-3 , the sample lysing incubation device further comprises a collection mechanism 81 which is detachably mounted on the base 90, and the base 90 is provided with a through hole 91, and the waste reagent plate and consumables can be thrown into the collection mechanism 81 through the through hole 91, so as to keep the sample lysing incubation device clean and reduce the risk of sample pollution.

[0058] In some embodiments, the collection mechanism 81 is pullably mounted on one end of the base 90, and the collection mechanism 81 is a trolley structure with a collection cavity, and the end of the base 90 is provided with a guide plate for guiding the collection mechanism 81.

[0059] In some embodiments, referring to Figures 1-3 , the base 90 is protrudingly provided with a funnel-shaped material guide 82 which is in communication with the through hole 91 of the base 90, so as to facilitate the accurate throwing of the waste reagent plate and consumables into the collection mechanism 81, and at the same time effectively reduce the probability of cross contamination.

[0060] In some embodiments, referring to Figures 1-3 , the film tearing device 70 and the film sealing device 40 are pullably mounted on the base 90 and can move close to and away from the track 61, and when pulled out, they move away from the track 61, which facilitates the manual addition of consumables required by the film tearing device 70 and the film sealing device 40.

[0061] In some embodiments, referring to Figures 1-3 , the sample lysing incubation device further comprises a code scanning gun 83 which is arranged on the interactive shelf 12 or on the base 90 and close to the interactive shelf 12, so as to perform the code scanning operation first at the beginning of the automatic process, thereby reducing the error rate.

[0062] In some embodiments, referring to Figures 1-3 , the sample lysing incubation device can further comprise a transfer plate which can temporarily place the sample plate clamped by the mechanical hand 62, so as to adjust the clamping posture and clamping position, and also solve the limitation of space and the movable angle range of the mechanical hand 62, such as adjusting the clamping posture by using the transfer plate during the process of transporting the sample plate after feeding to the centrifugal device 20, and then smoothly placing the sample plate into the feeding position of the centrifugal device 20.

[0063] In some embodiments, referring to Figures 1-3 , the sample lysing incubation device further comprises a calibration protrusion 86 which serves as a base point of the mechanical hand 62, so as to correct and adjust the mechanical hand 62, which is beneficial to ensure the accuracy and ensure the smooth progress of full automation.

[0064] The following is an example of a fecal sample undergoing lysis incubation, and the use of the sample lysis incubation device is described:

[0065] Before starting the automatic process, manually fill the reagent plate and consumables into the storage mechanism 11, then manually load the sample plate to be processed into the corresponding position of the interactive rack 12. Then initialize the device, and then start the automatic process. The automatic process is as follows:

[0066] (1) The manipulator 62 carries the sample plate on the interactive rack 12 to the centrifugal device 20 for centrifugation;

[0067] (2) The manipulator 62 carries the sample plate in the centrifugal device 20 to the film tearing device 70 for film tearing;

[0068] (3) The manipulator 62 carries the sample plate on the film tearing device 70 and the reagent plate and consumables in the storage mechanism 11 to the pipetting device to perform pipetting;

[0069] (4) The manipulator 62 carries the sample plate in the pipetting device to the film sealing device 40 for film sealing, and carries the reagent plate and consumables to the guide channel 82 for discarding;

[0070] (5) The manipulator 62 carries the sample plate on the film sealing device 40 to the centrifugal device 20 for centrifugation;

[0071] (6) The manipulator 62 carries the sample plate on the centrifugal device 20 to the film tearing device 70 for film tearing;

[0072] (7) The manipulator 62 carries the sample plate on the film tearing device 70 and the reagent plate and consumables in the storage mechanism 11 to the pipetting device to perform pipetting;

[0073] (8) The manipulator 62 carries the sample plate in the pipetting device to the temperature control mechanism 51 for temperature bath, and cools to room temperature after temperature bath, and carries the reagent plate and consumables to the guide channel 82 for discarding;

[0074] (9) The manipulator 62 carries the sample plate on the temperature control mechanism 51 and the reagent plate and consumables in the storage mechanism 11 to the pipetting device to perform pipetting;

[0075] (10) The manipulator 62 carries the sample plate on the pipetting device to the unloading area of the interactive rack 12, and carries the reagent plate and consumables to the guide channel 82 for discarding.

[0076] The sample lysis incubation device provided by the embodiment can be used in a fecal sample lysis incubation process, has the functions of pipetting, film sealing, film tearing, centrifugation, temperature bathing, oscillation, temporary storage and the like, and can realize full-process automation through reasonable arrangement, can complete the whole experiment process instead of manual operation, and can be used for completing a medium-high-throughput unattended automatic platform for CRC, Meta and HP.

[0077] The above application of specific examples to the utility model is described, only for help to understand the utility model, and not to limit the utility model. For the skilled person in the art to which the utility model belongs, according to the idea of the utility model, a number of simple deductions, deformations or substitutions can be made.

Claims

1. A sample lysis incubation apparatus, characterized by, The sample lysing and incubating device comprises: a sample placing device for placing a sample plate to be treated and reagents and consumables required for lysing and incubation; a centrifugal device for centrifuging the sample plate to be treated; a dispensing device for automatically dispensing reagents to the sample plate after centrifugation; a film sealing device for automatically sealing the sample plate containing samples and reagents after dispensing; a sample processing device comprising at least one of a temperature control mechanism for temperature control of the sample plate containing samples and reagents and a shaking mechanism for shaking the sample plate containing samples and reagents; and a transfer device, at least two of the sample placing device, the centrifugal device, the dispensing device, the film sealing device and the sample processing device being within the transfer range of the transfer device, the transfer device being used for automatically transferring the sample plate.

2. The sample lysis incubation apparatus of claim 1, wherein, The transfer device comprises a track and a mechanical hand installed on the track and movable along the track, and the sample placing device, the centrifugal device, the dispensing device, the film sealing device and the sample processing device are arranged on at least one side of the track.

3. The sample lysis incubation apparatus of claim 2, wherein, The track is a straight track, the sample processing device comprises the temperature control mechanism and the shaking mechanism, the temperature control mechanism, the shaking mechanism, the dispensing device and the centrifugal device are arranged on one side of the track, and the film sealing device is arranged on the other side of the track.

4. The sample lysis incubation apparatus of claim 3, wherein, Both the temperature control mechanism and the shaking mechanism are multiple, and in the moving direction of the mechanical hand along the track, the multiple temperature control mechanisms and the multiple shaking mechanisms are arranged in a row.

5. The sample lysis incubation apparatus of claim 4, wherein, The dispensing device and the centrifugal device are arranged in a row in the moving direction of the mechanical hand along the track, and the temperature control mechanism and the shaking mechanism are located between the track and the dispensing device.

6. The sample lysis incubation apparatus of any one of claims 3-5, wherein, The storage device comprises a storage mechanism for placing consumables and reagent plates and an interaction rack having multiple placing parts for positioning sample plates to be treated, and the storage mechanism and the interaction rack are within the transfer range of the transfer device.

7. The sample lysis incubation apparatus of claim 6, wherein, The storage mechanism and the film sealing device are arranged on the same side of the track, and in the moving direction of the mechanical hand along the track, the interaction rack is arranged at one end of the track and is spaced apart from the track.

8. The sample lysis incubation apparatus of claim 7, wherein, The storage device further comprises a temporary storage rack for receiving reagent plates and consumables of the storage mechanism, the temporary storage rack is adjacent to the dispensing device and is arranged on the same side of the track, and in the moving direction of the mechanical hand along the track, the centrifugal device, the dispensing device and the temporary storage rack are arranged in a row in sequence.

9. The sample lysis incubation apparatus of claim 7, wherein, The sample lysing and incubating device further comprises a film tearing device for tearing the film of a sample plate or a well plate covered with film, the film tearing device and the film sealing device are arranged adjacent to each other on the same side of the track. The sample placing device, the centrifugal device, the liquid separating device, the film sealing device, the film tearing device and the sample processing device are all within the transfer range of the mechanical arm, and the mechanical arm automatically transfers the sample plate between the devices and mechanisms.

10. The sample lysis incubation apparatus of any one of claims 1-5, wherein, The sample lysing and incubating device further comprises a base, and the centrifugal device, the liquid separating device, the film sealing device and the sample processing device are all mounted on the base. The sample lysing and incubating device further comprises a storage mechanism which is detachably mounted on the base, and the base is provided with a through hole through which the waste reagent plate and consumables can be put into the storage mechanism.

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