Device for rapidly removing Brucella in insect body with small piercing-sucking mouthpart or filing-sucking mouthpart

Abstract

The utility model discloses a device for quickly removing Brucella in small piercing-sucking mouthpart or filing-sucking mouthpart insects, which belongs to the field of biotechnology, and comprises a glass bottle, a degerming liner and a bottle cap, the color of the glass bottle is blue or yellow, the degerming liner is arranged in the glass bottle, the degerming liner is made of a transparent material, and the bottle cap is arranged in the glass bottle. The bottle cap is in threaded connection with the top end of the glass bottle, and a gauze element is arranged on the bottle cap. The insect killing device is simple in structure and convenient to use, insects are attracted through the blue or yellow glass bottle, the insects are attached to the bacteria removing film of the bacteria removing inner container, bacteria removing is conducted on the insects through the bacteria removing liquid on the bacteria removing film, the activity of the insects is reduced, and the survival rate during bacteria removing is improved.

Description

Technical Field

[0001] This invention belongs to the field of biotechnology, and in particular relates to a device for rapidly removing Buhnerella bacteria from the bodies of small insects with piercing-sucking or rasping-sucking mouthparts. Background Technology

[0002] Piercing-sucking insects insert their stylets into plant tissues or animal skin to directly suck sap or blood from within or between cells. Aphids, for example, insert their stylets into the phloem sieve tube cells of plants to extract sap rich in sugars and other nutrients. They exhibit selectivity in choosing their food sources. Some piercing-sucking insects specialize in parasitizing specific plant species or animal hosts. For instance, whiteflies prefer to feed on the sap of various vegetable crops, including those in the Solanaceae and Cucurbitaceae families. Piercing-sucking insects cause nutrient loss in plants. Continuous sap-sucking by insects leads to significant nutrient depletion, resulting in weakened plant growth. For example, large numbers of aphids feeding on plant sap can cause yellowing leaves and stunted growth. They can also damage plant vascular tissues. The piercing action of their stylets can damage sieve tubes and xylem vessels, affecting the normal transport of water and nutrients. Furthermore, they can transmit plant viruses. Many piercing-sucking insects are vectors for plant viruses. For example, when leafhoppers feed on the sap of virus-infected plants and then transfer to healthy plants, they spread the virus, causing plant viral diseases and resulting in serious losses to agricultural production. Insect saliva can also cause physiological disorders in plants. Certain components in insect saliva may interfere with normal plant metabolism, such as causing hormonal imbalances and leading to abnormal growth, such as leaf curling and tumor-like protrusions.

[0003] Raker-sucking insects prefer tender parts of plants, such as growing points, young leaves, young flowers, and young fruits. This is because the epidermis of these parts is relatively thin and the cells are rich in sap, making them easier to raker and suck. Some thrips can act as vectors for viruses, such as tomato spotted wilt virus. After feeding on virus-infected plants, thrips can transfer to healthy plants, spreading the virus and causing plant viral diseases, leading to widespread plant outbreaks.

[0004] In agricultural production, pathogens carried by some pests (such as Buchenella) can spread to crops, leading to crop diseases and affecting crop yield and quality. Furthermore, in biological experiments, insect-borne pathogens may interact with the target bacterial strain, interfering with experimental results. For example, when studying the insect immune system or the function of specific genes, the presence of pathogens can complicate and make the results difficult to analyze. For insect cell culture experiments requiring a sterile environment, insect-borne pathogens can cause cell contamination, leading to experimental failure.

[0005] Existing sterilization methods include chemical sterilization, which typically requires a long processing time and is complex due to the disorderly flight and movement of insects in specific sterilization environments. High-temperature sterilization is a commonly used physical sterilization method, but for small insects, high temperatures may cause death or damage to their physiological structures. For example, high temperatures may destroy the protein structure of insects, affecting their survival ability.

[0006] There is an urgent need for those skilled in the art to propose a highly efficient and safe sterilization device specifically for small insects. This device should be able to effectively remove various pathogens carried by insects without harming them, while also being easy and quick to operate, and capable of meeting the needs of different fields. Utility Model Content

[0007] In view of this, the present invention provides a device for rapidly removing Bukhnerella bacteria from the bodies of small piercing-sucking or rasping-sucking insects, in order to solve the above-mentioned problems.

[0008] To achieve the above objectives, the present invention adopts the following technical solution:

[0009] A device for rapidly removing Buchenella bacteria from the bodies of small piercing-sucking or rasping-sucking insects includes: a glass bottle, a sterilizing inner liner, and a bottle cap. The glass bottle is blue or yellow in color. The sterilizing inner liner is disposed inside the glass bottle and is made of transparent material. The bottle cap is threaded to the top of the glass bottle and is provided with a mesh screen.

[0010] Furthermore, the top of the sterilization inner liner is provided with a flange, which overlaps the edge of the bottle mouth, and the inner top wall of the bottle cap abuts against the upper surface of the flange.

[0011] Furthermore, it also includes a sterilization membrane, which is attached to the inner sidewall and inner bottom wall of the sterilization liner, and the sterilization membrane is coated with a sterilization solution.

[0012] Furthermore, the antibacterial membrane is a Parafilm membrane, which, when stretched, becomes adhesive and adheres to the inner sidewall and inner bottom wall of the antibacterial inner liner.

[0013] Furthermore, the disinfectant solution is a rifampicin sucrose solution.

[0014] Furthermore, the mesh count of the yarn is 150.

[0015] The beneficial effects of this utility model are as follows:

[0016] This invention uses a blue or yellow glass bottle, the color of which attracts insects. Insects with piercing-sucking or rasping mouthparts are placed inside the sterilization liner, which is then placed inside the glass bottle. This allows the insects to adhere to a sterilization membrane coated with a sterilizing solution, which sterilizes the insects. The blue or yellow glass bottle's attraction to insects reduces their activity, increasing their survival rate during sterilization. A mesh screen on the bottle cap ensures ventilation within the space formed by the cap and the sterilization liner, preventing insect death caused by poor ventilation in traditional devices, further improving the survival rate of piercing-sucking or rasping mouthparts. Furthermore, the glass bottle, sterilization liner, and cap of this invention have a simple structure and are easy to use. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0018] Figure 1 This is a schematic diagram of a device for rapidly removing Bukhnerella bacteria from the bodies of small piercing-sucking or rasping-sucking insects.

[0019] In the figure:

[0020] 10-Glass bottle, 20-Sterilized inner liner, 21-Flanged edge, 30-Bottle cap, 40-Gauze mesh, 50-Sterilized film. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0022] Example 1

[0023] See attached document Figure 1As shown, a device for rapidly removing Buchenella bacteria from small piercing-sucking or rasping-sucking insects includes: a glass bottle 10, a sterilizing inner liner 20, and a bottle cap 30. The glass bottle 10 is blue or yellow, with blue having a wavelength of 440–493 nm and yellow having a wavelength of 560–600 nm. The blue or yellow glass bottle 10 is attracted to small piercing-sucking or rasping-sucking insects. The sterilizing inner liner 20 is disposed inside the glass bottle 10 and is made of transparent material. The bottle cap 30 is threaded onto the top of the glass bottle 10 and has a mesh 40. The attraction of the blue or yellow glass bottle 10 to insects reduces insect activity and improves the survival rate during sterilization. The mesh 40 on the bottle cap 30 ensures that the space enclosed by the bottle cap 30 and the sterilizing inner liner 20 remains breathable, avoiding insect death caused by poor ventilation in traditional devices, further improving the survival rate of piercing-sucking or rasping-sucking insects.

[0024] In one optional embodiment, the top of the sterilization inner liner 20 is provided with a flange 21, which overlaps the edge of the bottle mouth of the glass bottle 10. The inner top wall of the bottle cap 30 abuts against the upper surface of the flange 21. After the bottle cap 30 is screwed tightly onto the bottle mouth of the glass bottle 10, the sterilization inner liner 20 can be fixed. Small piercing-sucking or rasping-sucking insects are attracted by the blue or yellow glass bottle 10 and attach to the wall of the sterilization inner liner 20. Applying sterilization liquid to the wall of the sterilization inner liner 20 can sterilize the small piercing-sucking or rasping-sucking insects.

[0025] In one optional embodiment, a device for rapidly removing Buchenella bacteria from the bodies of small piercing-sucking or rasping-sucking insects further includes a sterilizing membrane 50, which adheres to the inner sidewall and bottom wall of a sterilizing inner liner 20. The membrane 50 is coated with a sterilizing solution. Small piercing-sucking or rasping-sucking insects are attracted to the blue or yellow glass bottle 10 and adhere to the sterilizing membrane 50. The sterilizing solution on the membrane 50 sterilizes the insects. The sterilizing membrane 50 is removable and replaceable within the sterilizing inner liner 20, providing a new membrane 50 for each subsequent test, thus avoiding cross-contamination and improving the accuracy of the test.

[0026] In one optional embodiment, the sterilization membrane 50 is a Parafilm membrane. The Parafilm membrane becomes sticky after being stretched and is adhered to the inner side wall and inner bottom wall of the sterilization inner liner 20 after being stretched.

[0027] In one optional embodiment, the disinfectant is a rifampicin sucrose solution.

[0028] In one alternative embodiment, the mesh count of the yarn 40 is 150.

[0029] Example 2

[0030] A method for rapidly removing Buchenella bacteria from small piercing-sucking or rasping-sucking insects, specifically using the western flower thrips, is described below. The specific experimental steps are as follows:

[0031] S1. Sterilize the glass bottle 10, the sterilized inner liner 20 and the bottle cap 30, wash them and let them air dry;

[0032] S2. Stretch the Parafilm film and stick it to the inner wall and bottom wall of the sterile inner liner 20;

[0033] S3. Apply the rifampicin sucrose solution (prepare a 20% sucrose solution to prepare rifampicin to a concentration of 200 μg / ml) to the surface of the Parafilm membrane;

[0034] S4. Then use a brush or insect aspirator to collect the western flower thrips into the sterilized inner liner 20 until the sterilized inner liner 20 contains an appropriate number of western flower thrips.

[0035] S5. Place the sterilization inner liner 20 into the glass bottle 10, so that the flange 21 of the sterilization inner liner 20 overlaps the mouth of the glass bottle 10, and tighten the cap 30 on the top of the glass bottle 10. The mesh 40 keeps the sterilization inner liner 20 breathable. The blue or yellow glass cup 10 adheres to the Parafilm, reducing the activity of western flower thrips. The rifampicin sucrose solution kills Buchenori bacteria in western flower thrips, improving their survival rate.

[0036] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on its differences from other embodiments. Similar or identical parts between embodiments can be referred to interchangeably. For the apparatus disclosed in the embodiments, since they correspond to the methods disclosed in the embodiments, the description is relatively simple; relevant parts can be referred to the method section.

[0037] The above description of the disclosed embodiments enables those skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A device for rapid removal of Buchnera aphid endosymbionts from small piercing-sucking or rasping-sucking insects, comprising: include: The glass bottle (10), the sterile inner liner (20), and the bottle cap (30) are provided. The glass bottle (10) is blue or yellow. The sterile inner liner (20) is placed inside the glass bottle (10) and is made of transparent material. The bottle cap (30) is threaded to the top of the glass bottle (10) and is provided with a mesh (40).

2. The device for rapidly removing Bukhnerella bacteria from small piercing-sucking or rasping-sucking insects according to claim 1, characterized in that, The sterile inner liner (20) is provided with a flange (21) at the top. The flange (21) overlaps the edge of the bottle mouth of the glass bottle (10). The inner top wall of the bottle cap (30) abuts against the upper surface of the flange (21).

3. The device for rapidly removing Bukhnerella bacteria from small piercing-sucking or rasping-sucking insects according to claim 1, characterized in that, It also includes a sterilization membrane (50), which is attached to the inner sidewall and the inner bottom wall of the sterilization inner liner (20), and the sterilization membrane (50) is coated with a sterilization solution.

4. The device for rapidly removing Bukhnerella bacteria from small piercing-sucking or rasping-sucking insects according to claim 3, characterized in that, The sterilization membrane (50) is a Parafilm membrane, which, when stretched, becomes adhesive and adheres to the inner sidewall and inner bottom wall of the sterilization inner liner (20).

5. The device for rapidly removing Bukhnerella bacteria from small piercing-sucking or rasping-sucking insects according to claim 3, characterized in that, The sterilization solution is a rifampicin sucrose solution.

6. The device for rapidly removing Buchenella bacteria from small piercing-sucking or rasping-sucking insects according to claim 1, characterized in that, The mesh number of the yarn (40) is 150.

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