Experimental membrane washing device

Abstract

The utility model provides a membrane washing device for experiments, which comprises a hollow solution barrel (1) which is in fluid connection with a liquid discharge pistol (2) through a liquid suction pipe (3) to form a liquid adding channel; and the liquid discharging pistol (2) is in fluid connection with the liquid discharging pipe (4) to form a liquid discharging channel. According to the membrane washing device provided by the utility model, an electric pistol type liquid discharging and discharging mode is adopted, and the discharging and the adding of membrane washing liquid are controlled by pressing a liquid sucking button and a liquid discharging button, so that the problem of membrane pollution possibly occurring during liquid pouring and the influence on an experimental result caused by the impact of the membrane washing liquid on a membrane are avoided; and the problems of waste and messy table top possibly caused during liquid adding are also avoided, and the efficiency of film washing operation is improved.

Description

Technical Field

[0001] This utility model relates to a membrane washing device, and more particularly to a membrane washing device for laboratory use. Background Technology

[0002] This section provides only background information relevant to this disclosure and is not necessarily prior art.

[0003] Western blotting is a common protein detection assay in biological laboratories. Its procedures involve blocking incubation, primary antibody incubation, and secondary antibody incubation (a total of three incubations). Each incubation step is followed by membrane washing, and the washing is frequent (five washes after each incubation) and time-consuming (5-10 minutes per wash, totaling 25-50 minutes for five washes, and 75-150 minutes in total). Therefore, researchers need to change the washing buffer every 5-10 minutes. If there are many membranes, multiple sample boxes require different washing buffers, making the washing process tedious.

[0004] Currently, the PBST washing solution used in laboratory membrane washing is typically stored in large 5L containers due to the large volume of washing solution used. It is poured directly into the washing box for replacement. This process is problematic because: firstly, the large volume of washing solution makes the heavy containers difficult to control, leading to overfilling, unnecessary waste, and spillage; secondly, the washing solution can cause unsatisfactory experimental results (e.g., NC membranes are easily dispersed by the washing solution, resulting in unattractive color development); and thirdly, each time a membrane is washed, multiple washing boxes need to be removed from the shaker, and 10-20ml of washing solution needs to be poured from the 5L container into each box. This is time-consuming and labor-intensive, and when the number of membranes transferred is large, the risk of solution spillage and membrane impact increases.

[0005] It should be noted that the information disclosed in the background section above is only used to enhance the understanding of the background of this disclosure, and therefore may include information that does not constitute prior art known to those skilled in the art. Utility Model Content

[0006] Purpose of the invention: The technical problem to be solved by this utility model is to provide an experimental membrane washing device to address the shortcomings of the existing technology.

[0007] To solve the above-mentioned technical problems, this utility model discloses an experimental membrane washing device, comprising:

[0008] A hollow solution tank is provided, wherein the solution tank is fluidly connected to a dispensing pistol via a suction tube to form a dispensing channel; the dispensing pistol is fluidly connected to a dispensing pipe to form a dispensing channel.

[0009] Furthermore, the liquid discharge pistol includes:

[0010] The device includes an inlet, an outlet, and a nozzle; wherein the inlet is fluidly connected to one end of the suction tube, and the outlet is fluidly connected to one end of the discharge tube.

[0011] The first channel is between the liquid inlet and the muzzle, through which the substance in the suction tube is discharged through the muzzle of the discharge pistol;

[0012] The second channel is located between the liquid outlet and the gun nozzle, through which external substances are drawn into the liquid discharge pipe.

[0013] Furthermore, the liquid discharge pistol includes:

[0014] A hollow gun body, with the liquid inlet and the liquid outlet at one end and the gun muzzle at the other end;

[0015] The gun body is equipped with an internal liquid inlet pipe, an internal liquid outlet pipe, and a solenoid valve. One end of the internal liquid inlet pipe and the internal liquid outlet pipe are the liquid inlet and the liquid outlet, respectively, and the other end is fluidly connected to the muzzle through the solenoid valve.

[0016] Furthermore, the solenoid valve is a dual-channel solenoid valve, wherein the first channel of the solenoid valve is used to discharge the substance in the inlet tube of the gun through the gun nozzle, and the second channel of the solenoid valve is used to draw external substances into the outlet tube of the gun through the gun nozzle.

[0017] Furthermore, the liquid-discharging pistol also includes:

[0018] A switch button, which is electrically connected to the solenoid valve.

[0019] Furthermore, the liquid-discharging pistol also includes:

[0020] A battery, which is electrically connected to the solenoid valve.

[0021] Furthermore, the liquid-discharging pistol also includes:

[0022] A hollow gun handle is fixedly connected to the gun body, the switch button is fixed on the outer wall of the gun handle, and the battery is disposed inside the gun handle.

[0023] Furthermore, the experimental membrane washing device also includes:

[0024] Flow meters are installed on the inlet pipe and outlet pipe inside the gun.

[0025] Furthermore, the flow meter is a dual-channel flow meter, wherein the first channel of the flow meter is used to measure the flow rate of the liquid inlet pipe inside the gun, and the second channel of the flow meter is used to measure the flow rate of the liquid outlet pipe inside the gun.

[0026] Furthermore, the liquid-discharging pistol also includes:

[0027] The touchscreen is electrically connected to the battery, the flow meter, and the solenoid valve.

[0028] Beneficial effects:

[0029] The membrane washing device proposed in this utility model adopts an electric pistol-type liquid discharge method. By pressing the liquid suction and liquid discharge buttons, the discharge and addition of membrane washing liquid are controlled, avoiding membrane contamination problems that may occur when pouring liquid and the impact of membrane washing liquid on the membrane on experimental results. It also avoids the waste and table mess that may occur when adding liquid, thus improving the efficiency of membrane washing operation. Attached Figure Description

[0030] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments, and the advantages of the present invention in the above and / or other aspects will become clearer.

[0031] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0032] Figure 2 This is a schematic diagram of the structure of a liquid-discharging pistol.

[0033] In the diagram, 1 is the solution tank, 2 is the dispensing pistol, 3 is the suction tube, 4 is the dispensing tube, 5 is the tidal shaker, and 6 is the membrane washing box.

[0034] 11 is the barrel body, 12 is the barrel lid, and 13 is the gun hanging part;

[0035] 21 is the gun body, 22 is the muzzle, 23 is the solenoid valve, 24 is the flow meter, 25 is the switch button, 26 is the gun handle, 27 is the battery, 28 is the touch screen; 211 is the inlet pipe inside the gun, and 212 is the outlet pipe inside the gun. Detailed Implementation

[0036] The device in this technical solution mainly consists of a solution tank 1, a liquid discharge pistol 2, and rubber tubing (liquid suction tube 3 and liquid discharge tube 4). A schematic diagram of the device is shown below. Figure 1 As shown.

[0037] The liquid inlet of the liquid discharge pistol 2 is fluidly connected to one end of the liquid suction tube 3, and the other end of the liquid suction tube 3 is inserted into the bottom of the solution tank 1 to transport the liquid in the solution tank 1 into the liquid discharge pistol 2.

[0038] The outlet of the drain pistol 2 is fluidly connected to one end of the drain pipe 4, and the other end of the drain pipe 4 is connected to a water tank for discharging waste liquid.

[0039] The solution tank 1 includes a hollow tank body 11, and a lid 12 is provided on the top of the tank body 11. The lid 12 is threadedly connected to the tank body 11 to prevent contamination of the washing solution. One end of the suction tube 3 is connected to the solution tank 1, passes through the opening on the lid 12, and is placed at the bottom of the tank body 11.

[0040] The side of the solution tank 1 is also fixed with a gun-hanging component 13 for hanging the draining pistol 2. The gun-hanging component 13 can be a hook, buckle, etc.

[0041] Fluid discharge pistol 2 Figure 2 As shown, it consists of a hollow gun body 21 and a gun handle 26. One end of the gun body 21 is provided with a muzzle 22 (for suction and discharge of liquid), and the other end is fluidly connected to the suction pipe 3 and the discharge pipe 4 respectively.

[0042] The gun body 21 is equipped with an internal liquid inlet pipe 211, an internal liquid outlet pipe 212, a solenoid valve 23, and a flow meter 24 (used to control the inlet and outlet liquid volumes).

[0043] One end of the liquid inlet pipe 211 inside the gun is the liquid inlet, which is fluidly connected to the liquid suction pipe 3; the other end of the liquid inlet pipe 211 inside the gun passes through the flow meter 24 and is fluidly connected to the solenoid valve 23.

[0044] One end of the liquid outlet tube 212 inside the gun is the liquid outlet, which is fluidly connected to the drain tube 4; the other end of the liquid outlet tube 212 inside the gun passes through the flow meter 24 and is fluidly connected to the solenoid valve 23.

[0045] The flow meter 24 is a dual-channel flow meter, with one channel used to measure the flow rate of the liquid outlet pipe 212 inside the gun and the other channel used to measure the flow rate of the liquid inlet pipe 211 inside the gun.

[0046] The solenoid valve 23 is a dual-channel solenoid valve. One channel is used to discharge the liquid in the inlet tube 211 of the gun through the nozzle 22, and the other channel is used to draw external liquid into the inlet tube 211 of the gun through the nozzle 22.

[0047] A switch button 25 (used to control the intake and discharge of liquid) is fixed on the outer wall of the gun handle 26, and a battery 27 is fixed inside. The solenoid valve 23 is electrically connected to the battery 27 through the switch button 25, controlling the liquid to be drawn in through the suction tube 3 to enter the membrane washing box 6, and then to be drawn in waste liquid from another membrane washing box 6 and discharged and discarded through the drain pipe 4.

[0048] A touch screen 28 is also fixed on the outer wall of the gun handle 26. The touch screen 28 is electrically connected to the battery 27, the flow meter 24 and the solenoid valve 23 respectively. It is used to display the battery status, flow data and other related setting information, and control the solenoid valve 23 according to the settings.

[0049] In use, prepare the washing solution in solution tank 1 and place it next to the tidal shaker 5, which has multiple washing boxes 6. Set the dispensing pistol 2 to the dispensing volume and pause time, and press the switch once to first aspirate the liquid and then dispense a measured amount at intervals. For example, aspirate the liquid from washing box 6, dispense 15ml, pause for 2 seconds to facilitate changing the transfer box, aspirate the liquid from washing box 6 again, dispense 15ml, and pause for 2 seconds. When the last washing box 6 is reached, press the switch again to stop the liquid aspiration and dispensing operation.

[0050] Because this method uses an electric dispensing pistol 2 to connect the solution tank 1 and the membrane washing box 6, there is no need to move the washing solution tank 1 when adding washing solution. The inflow and outflow of washing solution is controlled by a jog metering system, so there is no situation where washing solution splashes or is added too much, causing reagent waste and impact on the membrane. The liquid exchange between multiple membrane washing boxes 6 can be completed by moving the dispensing pistol 2, which reduces the experimental operation time, avoids the problem of dirt and mess on the experimental table and membrane contamination, not only stabilizes the experimental results, but also improves the efficiency of the experimental personnel.

[0051] The dispensing pistol 2 in this device is rechargeable. To charge, remove the rubber tubing connected to the pistol. After charging, reinstall the tubing according to the inlet and outlet positions. In use, place the other end of the suction tube 3 of the dispensing pistol 2 into the solution tank 1, and the other end of the dispensing tube 4 into the water tank. Set the required parameters on the gun body (dispensing volume and pause duration, etc.). Press the switch button 25 once to start suction (dispensing) and dispensing (adding) operations, and press it again to stop operation. When not in use, the dispensing pistol 2 can be hung on the gun-hanging component 13 on the side wall of the solution tank 1. To prepare the solution, remove the tank cap 12, prepare the solution, tighten the cap 12, and insert the suction tube 3 to the bottom of the solution tank 1.

[0052] This utility model provides a concept and method for an experimental membrane washing device. Many methods and approaches exist for implementing this technical solution; the above description is merely a preferred embodiment of this utility model. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of this utility model, and these improvements and modifications should also be considered within the scope of protection of this utility model. All components not explicitly stated in this embodiment can be implemented using existing technology.

Claims

1. An experimental membrane washing apparatus, characterized by, include: A hollow solution tank (1) is fluidly connected to a dispensing pistol (2) via a suction pipe (3) to form a liquid filling channel; the dispensing pistol (2) is fluidly connected to a dispensing pipe (4) to form a dispensing channel.

2. The experimental membrane washing apparatus according to claim 1, wherein The drain pistol (2) includes: Liquid inlet, liquid outlet and nozzle (22); wherein, the liquid inlet is fluidly connected to one end of the suction tube (3), and the liquid outlet is fluidly connected to one end of the drain tube (4); The first channel is between the liquid inlet and the muzzle (22), through which the substance in the suction tube (3) is discharged through the muzzle (22) of the discharge pistol (2); The second channel is located between the liquid outlet and the gun nozzle (22), through which external substances are drawn into the drain pipe (4).

3. The experimental membrane washing apparatus according to claim 2, wherein The drain pistol (2) includes: A hollow gun body (21) is provided with the liquid inlet and the liquid outlet at one end of the gun body (21) and the gun muzzle (22) at the other end; The gun body (21) is provided with an internal liquid inlet pipe (211), an internal liquid outlet pipe (212) and a solenoid valve (23). One end of the internal liquid inlet pipe (211) and the internal liquid outlet pipe (212) are the liquid inlet and the liquid outlet, respectively, and the other end is fluidly connected to the gun muzzle (22) through the solenoid valve (23).

4. The experimental membrane washing apparatus according to claim 3, wherein The solenoid valve (23) is a dual-channel solenoid valve. The first channel of the solenoid valve is used to discharge the substance in the inlet pipe (211) of the gun through the gun nozzle (22), and the second channel of the solenoid valve is used to draw external substances into the outlet pipe (212) of the gun through the gun nozzle (22).

5. The experimental membrane washing apparatus according to claim 3, wherein The liquid discharge pistol (2) further includes: A switch button (25) is electrically connected to the solenoid valve (23).

6. The experimental membrane washing device according to claim 5, characterized in that, The liquid discharge pistol (2) further includes: Battery (27), which is electrically connected to the solenoid valve (23).

7. The experimental membrane washing apparatus according to claim 6, wherein The liquid discharge pistol (2) further includes: A hollow gun handle (26) is fixedly connected to the gun body (21), the switch button (25) is fixed on the outer wall of the gun handle (26), and the battery (27) is disposed inside the gun handle (26).

8. The experimental membrane washing apparatus according to claim 6, wherein The experimental membrane washing device further includes: Flow meters (24) are installed on the inlet pipe (211) and outlet pipe (212) inside the gun.

9. The experimental membrane washing apparatus according to claim 8, wherein The flow meter (24) is a dual-channel flow meter, wherein the first channel of the flow meter is used to measure the flow rate of the inlet pipe (211) inside the gun, and the second channel of the flow meter is used to measure the flow rate of the outlet pipe (212) inside the gun.

10. The experimental membrane washing apparatus according to claim 8, wherein The liquid discharge pistol (2) further includes: The touch screen (28) is electrically connected to the battery (27), the flow meter (24) and the solenoid valve (23).

Images