Vortex oscillation type reactor for preparing mesenchymal stem cell spheres

Abstract

The utility model discloses a vortex oscillation type reactor for preparing mesenchymal stem cell spheres, which comprises a base, a motor and an oscillation support are mounted on the base, a cam is connected onto an output shaft of the motor, the motor drives the cam and the oscillation support to rotate synchronously, and a container bottle fixing support is mounted on the oscillation support through a spring. The container bottle fixing support is used for installing a replaceable container bottle, the motor is provided with a controller, and the controller is used for controlling the rotating speed, the forward and reverse rotation direction and timing start and stop of the motor. According to the utility model, the cells are inhibited from adhering to the wall in a special vortex oscillation mode, the cells are promoted to naturally gather to form spheres, the direct shear damage of mechanical stirring to the cells is reduced, and the cell activity and the sphere forming efficiency are improved; the rotating speed and the direction of the motor can be controlled, the vortex intensity and the oscillation frequency are accurately regulated and controlled, the structure is simple and compact, operation is convenient and fast, and the device is suitable for small laboratories.

Description

Technical Field

[0001] This utility model relates to the field of biomedical equipment technology, specifically to a vortex oscillating reactor for preparing mesenchymal stem cell spheres. Background Technology

[0002] Mesenchymal stem cells (MSCs) are a type of adult stem cell with self-renewal and multi-lineage differentiation potential, and are widely used in tissue engineering, regenerative medicine, and cell therapy. Compared with traditional two-dimensional planar culture, stem cell spheres cultured under three-dimensional conditions have more significant advantages in terms of stemness maintenance, differentiation potential, immune regulation, and paracrine function. Therefore, the preparation technology of MSC spheres is receiving increasing attention.

[0003] Currently, stirred culture is widely used due to its relatively simple operation and ease of scale-up. However, existing stirred culture equipment has some common problems: on the one hand, traditional mechanical stirrers can easily generate large shear forces, which may damage cells, reduce cell viability and spheroidization efficiency, and make it difficult to create a stable flow field environment that can effectively inhibit cell adhesion; on the other hand, the adjustable range of stirring parameters in many devices is limited or fixed, making it difficult to meet the optimization needs of different types of stem cells or different spheroidization stages. In addition, some equipment has a complex structure, is inconvenient to clean and sterilize, poses a risk of contamination, and is also bulky and not convenient to operate, making it unsuitable for the flexible application needs of small laboratories.

[0004] Therefore, developing a mesenchymal stem cell sphere preparation device that can effectively inhibit cell adhesion, reduce shear damage, promote cell spheroidization, has flexibly adjustable parameters, and is simple, compact, and easy to operate is of great practical significance. Utility Model Content

[0005] The purpose of this invention is to overcome the shortcomings of the prior art and provide a vortex oscillation reactor for the preparation of mesenchymal stem cell spheres. This reactor inhibits cell adhesion and promotes cell sphere formation through oscillation. It is simple in structure, easy to operate, and allows for flexible adjustment of oscillation frequency and amplitude, making it suitable for the preparation needs of different types of mesenchymal stem cell spheres.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] A vortex oscillation reactor for preparing mesenchymal stem cell spheres includes: a base, on which a motor and an oscillation support are mounted; a cam is connected to the output shaft of the motor; the motor drives the cam and the oscillation support to rotate synchronously; a container bottle fixing bracket is mounted on the oscillation support via a spring; the container bottle fixing bracket is used to install replaceable container bottles; and the motor is equipped with a controller for controlling the motor's speed, forward and reverse direction, and timed start and stop.

[0008] Preferably, the controller includes: a speed regulator, a direction controller, and a timer; the speed regulator is electrically connected to the motor and is used to regulate the speed of the motor; the direction controller is electrically connected to the motor and is used to control the forward and reverse rotation of the motor; the timer is electrically connected to the motor and is used to control the timed start and stop of the motor.

[0009] Preferably, the outer edge of the cam contacts the inner wall of the oscillating bracket, so as to generate a periodic oscillating force on the oscillating bracket when the motor rotates.

[0010] Preferably, the container bottle fixing bracket includes an upper fixing ring and a lower fixing ring, the upper fixing ring and the lower fixing ring are connected by at least three support rods, and the inner side of the upper fixing ring and the lower fixing ring are provided with rubber sleeves to fix the container bottle and prevent the container bottle from loosening during vibration.

[0011] Preferably, there are multiple springs, evenly distributed between the oscillation support and the container bottle fixing support, to provide oscillation buffering and rebound force.

[0012] Preferably, the container bottle is equipped with a diaphragm valve for gas exchange and addition or sampling of culture medium within the container bottle.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] This invention replaces traditional mechanical agitators with a unique vortex oscillation mixing method, generating a gentle and stable flow field that effectively inhibits cell adhesion and promotes natural cell aggregation into spheres. Simultaneously, it significantly reduces direct shear damage to cells, thereby improving cell survival rate and final sphere formation efficiency. Furthermore, the controller allows for precise control of the motor's speed, direction, and running time, enabling flexible adjustment of vortex intensity and oscillation frequency to meet the preparation needs of different types of mesenchymal stem cells or optimize culture conditions. Combined with its simple and compact overall structure, easily replaceable containers, and convenient operation, this device is easy to clean, reduces the risk of contamination, and is particularly suitable for efficient and low-cost mesenchymal stem cell sphere preparation research in small laboratory environments. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of this utility model.

[0016] In the diagram: 1-base, 2-motor, 3-cam, 4-vibration table support, 5-spring, 6-container bottle fixing support, 7-container bottle, 8-controller, 9-rubber sleeve, 10-diaphragm valve. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0018] Please see Figure 1 This utility model provides a vortex oscillating reactor for preparing mesenchymal stem cell spheres, including a base 1, on which a motor 2 and an oscillation support 4 are mounted. A cam 3 is connected to the output shaft of the motor 2, and the motor 2 drives the cam 3 and the oscillation support 4 to rotate synchronously. A container bottle fixing bracket 6 is mounted on the oscillation support 4 through a spring 5. The container bottle fixing bracket 6 is used to install replaceable container bottles 7. The motor 2 is equipped with a controller 8, which is used to control the speed, forward and reverse direction, and timed start and stop of the motor 2.

[0019] The controller 8 includes a speed regulator, a direction controller, and a timer. The speed regulator is electrically connected to the motor 2 and is used to adjust the speed of the motor 2, which can be adjusted between 50 and 500 rpm. The direction controller is electrically connected to the motor 2 and is used to control the forward and reverse direction of the motor 2. The timer is electrically connected to the motor 2 and is used to control the timed start and stop of the motor 2 to realize intermittent oscillation culture.

[0020] The outer edge of cam 3 contacts the inner wall of the oscillating support 4, generating a periodic oscillating force on the oscillating support 4 when the motor 2 rotates. Cam 3 is elliptical in shape, and its eccentric structure generates a periodic thrust during rotation, driving the oscillating support 4 to oscillate, forming a stable vortex field that effectively inhibits cell adhesion and promotes cell spheroidization.

[0021] The container bottle fixing bracket 6 includes an upper fixing ring and a lower fixing ring, which are connected by four support rods. Both the upper and lower fixing rings have rubber sleeves 9 on their inner sides to secure the container bottle 7 and prevent it from loosening during vibration. The rubber sleeves 9 are made of medical-grade silicone to increase friction and provide shock absorption.

[0022] Six springs 5 ​​are evenly distributed between the shaking support 4 and the container bottle fixing support 6 to provide shaking cushioning and rebound force. The springs 5 ​​have a moderate elastic coefficient, which can both transmit the shaking force and play a cushioning role to prevent excessive shaking from damaging the cells.

[0023] The container bottle 7 is equipped with a diaphragm valve 10 for gas exchange and the addition or sampling of culture medium within the container bottle 7. The diaphragm valve 10 uses a puncture-resistant self-sealing silicone diaphragm to ensure the airtightness and sterility of the culture environment, while also facilitating operation during the culture process.

[0024] The working principle of this invention is as follows: Mesenchymal stem cells are mixed with culture medium and injected into container bottle 7, which is then fixed on container bottle support 6. Motor 2 is started, driving cam 3 and oscillating support 4 to rotate synchronously. The eccentric motion of cam 3 generates a periodic thrust on oscillating support 4, which transmits the oscillating force to container bottle support 6 and container bottle 7 via spring 5, forming a vortex flow field. Under the action of this vortex flow field, suspended mesenchymal stem cells are inhibited from adhering to the container wall and naturally aggregate to form cell spheroids. By adjusting the speed, direction, and working time of motor 2 through controller 8, different intensities of vortex fields and oscillation effects can be obtained to meet the preparation needs of different types of mesenchymal stem cell spheroids.

[0025] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A mesenchymal stem cell sphere preparation vortex oscillation type reactor, characterized by, include: A base (1) is provided, on which a motor (2) and an oscillating bracket (4) are mounted. A cam (3) is connected to the output shaft of the motor (2). The motor (2) drives the cam (3) and the oscillating bracket (4) to rotate synchronously. A container bottle fixing bracket (6) is mounted on the oscillating bracket (4) via a spring (5). The container bottle fixing bracket (6) is used to install replaceable container bottles (7). The motor (2) is provided with a controller (8). The controller (8) is used to control the speed, forward and reverse direction, and timed start and stop of the motor (2).

2. The vortex shaking type reactor for preparing a mesenchymal stem cell sphere according to claim 1, wherein The controller (8) includes: a speed regulator, a direction controller and a timer. The speed regulator is electrically connected to the motor (2) and is used to adjust the speed of the motor (2). The direction controller is electrically connected to the motor (2) and is used to control the forward and reverse rotation of the motor (2). The timer is electrically connected to the motor (2) and is used to control the timed start and stop of the motor (2).

3. The vortex oscillating reactor for preparing mesenchymal stem cell spheres according to claim 1, characterized in that, The outer edge of the cam (3) contacts the inner wall of the oscillation bracket (4) to generate a periodic oscillation force on the oscillation bracket (4) when the motor (2) rotates.

4. The vortex shaking type reactor for preparing a mesenchymal stem cell sphere according to claim 1, wherein, The container bottle fixing bracket (6) includes an upper fixing ring and a lower fixing ring. The upper fixing ring and the lower fixing ring are connected by at least three support rods. The inner sides of the upper fixing ring and the lower fixing ring are provided with rubber sleeves (9) for fixing the container bottle (7) and preventing the container bottle (7) from loosening during vibration.

5. The vortex shaking type reactor for preparing a mesenchymal stem cell sphere according to claim 1, wherein, Multiple springs (5) are evenly distributed between the oscillation bracket (4) and the container bottle fixing bracket (6) to provide oscillation buffer and rebound force.

6. The reactor of claim 1, wherein, The container bottle (7) is equipped with a diaphragm valve (10) for the exchange of gas inside the container bottle (7) and the addition or sampling of culture medium.

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