Heating platform for hybridization

Abstract

The utility model belongs to the technical field of instrument heating, and provides a heating platform for hybridization, which comprises a platform, a groove body and a heating piece, the top end of the tank body is open, and two communicating ports are formed in the side wall of the tank body; the platform is arranged at an opening in the top end of the tank body, a groove for placing a sample glass slide is formed in the platform, and a cooling fin is arranged at the bottom end of the platform; and the heating piece is arranged in the groove body. When the heating platform is used, a sample glass slide is placed on the platform, water is added into the groove body through the communicating opening, then the water is heated to a preset temperature through the heating piece, heat is transferred to the cooling fins through the water, then the heat is transferred to the platform for heating, and the hybridization step of the sample is facilitated. And when cooling is needed, heated hot water is discharged through the communication port and is changed into normal-temperature water to cool the platform, so that the cooling efficiency is high. Meanwhile, in the heating and cooling process, part of water is evaporated, a wet environment is provided for the sample, and the probe on the sample glass slide is prevented from being evaporated.

Description

Technical Field

[0001] This utility model belongs to the field of instrument heating technology, specifically relating to a heating platform for hybridization. Background Technology

[0002] Paraffin-embedded tissue sections are the most widely used method in routine histological slide preparation. In pathology, they are often used to study, observe, and determine morphological changes in cells and tissues. When combined with fluorescence in situ hybridization (FISH), they can be used for gene localization and state analysis in cells.

[0003] In the process of combining paraffin-embedded tissue sections with FISH, the sample is paraffin-embedded tissue sectioned, and then undergoes dewaxing, penetration with a penetrant, digestion with pepsin, washing, and draining. Then, a probe is dropped onto the sample, and hybridization is performed.

[0004] In existing technologies, a heated stage is generally used for the hybridization step because hybridization is usually performed at high temperatures to promote the specific binding of the probe to the target sequence. However, when using a heated stage, the sample needs to be covered with a humidifying cover to maintain a moist environment during heating, in order to minimize probe evaporation and hybridization failure, which is cumbersome. Furthermore, after the sample is heated to 80-90°C on the heated stage during hybridization, it needs to be cooled to 40-50°C and maintained for 1-2 hours. Cooling is typically done using a fan, which is inefficient. Utility Model Content

[0005] To address the shortcomings of existing technologies, this invention provides a heating platform for hybridization, which can solve the aforementioned problems.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a heating platform for hybridization, comprising a platform, a tank, and a heating element;

[0007] The top of the tank is open, and at least two connecting ports are provided on the side wall of the tank;

[0008] The platform is located at the top opening of the tank, and the platform is provided with a groove for placing sample slides. The bottom of the platform is provided with heat sinks.

[0009] The heating element is disposed inside the tank.

[0010] Preferably, a liquid level sensor is also included, which is disposed within the tank.

[0011] Preferably, a temperature sensor is also included, which is used to detect the temperature of the platform.

[0012] Preferably, the platform sidewall is provided with a detection hole, and the temperature sensor probe is disposed in the detection hole.

[0013] Preferably, the platform is mounted on the tank by bolts.

[0014] Preferably, the inner wall of the tank is provided with a support platform, and the platform is mounted on the support platform by bolts.

[0015] Preferably, a support platform is provided at each of the four corners of the tank.

[0016] Preferably, the platform is provided with multiple grooves, which are distributed in a matrix.

[0017] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0018] This invention provides a heating platform for hybridization. In use, a sample slide is placed on the platform, water is added to the tank through the connecting port, and then the water is heated to a predetermined temperature by a heating element. The water then transfers heat to a heat sink, which in turn transfers it to the platform for heating, facilitating the hybridization process. When cooling is required, the heated hot water is drained through the connecting port and replaced with room temperature water to cool the platform, resulting in rapid cooling. Simultaneously, some water evaporates during the heating and cooling process, providing a humid environment for the sample and preventing the probes on the sample slide from evaporating. Attached Figure Description

[0019] Figure 1 A three-dimensional structural diagram of a heating platform for hybridization provided in an embodiment of this utility model;

[0020] Figure 2 An exploded structural diagram of a hybridization heating platform provided in an embodiment of this utility model;

[0021] Figure 3 A three-dimensional structural diagram of the tank and related parts of a hybridization heating platform provided for an embodiment of this utility model;

[0022] Figure 4 This is a three-dimensional structural diagram of a heat sink and related parts of a hybridization heating platform provided for an embodiment of the present invention.

[0023] The attached diagram lists the components represented by each number as follows:

[0024] 1. Sample slide;

[0025] 2. Platform;

[0026] 3. Tank body;

[0027] 4. Heating element;

[0028] 5. Temperature sensor;

[0029] 6. Liquid level sensor;

[0030] 7. Connecting port;

[0031] 8. Heat sink;

[0032] 9. Groove;

[0033] 10. Platform support. Detailed Implementation

[0034] To make the technical solutions and advantages of the embodiments of this application clearer, the exemplary embodiments of this application will be described in further detail below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not an exhaustive list of all embodiments. It should be noted that, unless otherwise specified, the embodiments and features in the embodiments of this application can be combined with each other.

[0035] This embodiment provides a heating platform for hybridization, including a platform 2, a tank 3, and a heating element 4.

[0036] The top of the tank 3 is open, and at least two connecting ports 7 are provided on the side wall of the tank 3.

[0037] For example, see Figure 1-2 The tank 3 has a box-like structure with an open top, allowing it to store water as a heat transfer medium. Two connecting ports 7 are located on the right side wall of the tank 3, communicating with the interior of the tank 3. These two ports serve as inlets and outlets, allowing for the addition and drainage of water into the tank 3. Alternatively, coolant or other fluids can be used instead of water as the heat transfer medium.

[0038] Platform 2 is located at the top opening of the tank 3. Platform 2 has a groove 9 for placing the sample slide 1 and a heat sink 8 at the bottom.

[0039] For example, see Figure 1-2 Platform 2 is placed over the opening at the top of the tank 3, and the heat sink 8 at the bottom of platform 2 is inserted into the tank 3 and immersed in water. The heat sink 8 can accelerate heat transfer efficiency. Multiple grooves 9 are formed on the upper surface of platform 2, and the grooves 9 are used to place the sample slide 1.

[0040] The heating element 4 is installed inside the tank 3.

[0041] For example, see Figure 3The heating element 4 is located at the bottom of the tank 3, below the heat sink 8, and will not interfere with the heat sink 8. The left side wall of the tank 3 has mounting holes for the heating element 4's pipeline to pass through. The heating element 4 can be a heating rod with a U-shaped structure; the higher the power of the heating rod, the higher the heating efficiency.

[0042] Based on the above structure, during use, the sample slide 1 is placed on the platform 2, water is added to the tank 3 through the connecting port 7, and then the water is heated to a predetermined temperature by the heating element 4. The water then transfers heat to the heat sink 8, and finally to the platform 2 for heating, facilitating the hybridization step of the sample. When cooling is required, the heated hot water is drained through the connecting port 7 and replaced with room temperature water to cool the platform 2, resulting in rapid cooling. Simultaneously, during the heating and cooling process, some water evaporates, providing a humid environment for the sample and preventing the probes on the sample slide 1 from evaporating.

[0043] Based on the above technical solution, in the technical solution provided in this embodiment, the heating platform for hybridization may also include a liquid level sensor 6, which is installed inside the tank 3.

[0044] For example, see Figure 1-3 The level sensor 6 is installed on the inner wall of the tank 3 to detect the liquid level in the tank 3, thereby monitoring the amount of water in the tank 3. A mounting hole for the pipeline of the level sensor 6 is provided on the right side wall of the tank 3.

[0045] In the technical solution provided in this embodiment, the hybridization heating platform may further include a temperature sensor 5, which is used to detect the temperature of the platform 2.

[0046] Furthermore, a detection hole is provided on the side wall of platform 2, and the temperature sensor 5 probe is installed in the detection hole.

[0047] For example, see Figure 1-3 The left side wall of platform 2 is equipped with a detection hole, and the temperature sensor 5 probe is inserted into the detection hole to detect the temperature of platform 2.

[0048] After platform 2 is placed over the opening at the top of tank 3, a U-shaped groove is provided on the left side wall of tank 3 to prevent interference between the temperature sensor 5's pipeline and the side wall of tank 3. The U-shaped groove extends upward to the top of the side wall of tank 3. This allows the temperature sensor 5's pipeline to be inserted downward into the U-shaped groove when platform 2 is placed over the opening at the top of tank 3, preventing interference and facilitating installation.

[0049] In the technical solution provided in this embodiment, the hybridization heating platform may further include a controller, which can be electrically connected to the heating element 4 and the temperature sensor 5 respectively. When the temperature sensor 5 detects that the temperature of the platform 2 has risen to a predetermined value, it transmits a signal to the controller, which then transmits the signal to the heating element 4. The heating element 4 then reduces its heating power or stops heating. This achieves automatic control of the heating element 4.

[0050] The connecting port 7 can be connected to the water tank via a pipeline, and a pump is installed on this pipeline. The pump can supply water to the connecting port 7, that is, add water to the tank 3. At this time, the controller can be electrically connected to the level sensor 6 and the pump respectively. When the level sensor 6 detects that the water level in the tank 3 has reached the predetermined value, it transmits a signal to the controller, which then transmits a signal to the pump, and the pump stops pumping water. This realizes automatic water addition by the pump.

[0051] In the technical solution provided in this embodiment, the platform 2 can be mounted on the groove 3 by bolts.

[0052] For example, see Figure 1-3 Each of the four corners of the tank 3 is provided with a support platform 10. The height of the support platform 10 is lower than the height of the side wall of the tank 3. The platform 2 is bolted to the support platform 10. That is, the support platform 10 is provided with screw holes, and the four corners of the platform 2 are provided with through holes. The end of the bolt passes through the through hole and is screwed into the screw hole, so that the platform 2 can be locked and fixed to the support platform 10.

[0053] Platform 2 can cover the entire opening of the tank 3, improving the heat preservation effect. Platform 2 can be equipped with multiple vents to allow water vapor to escape during the heating process, ensuring ambient humidity.

[0054] In the technical solution provided in this embodiment, the platform 2 is provided with multiple grooves 9, which are distributed in a matrix.

[0055] For example, see Figure 1-3 Platform 2 has two rows of grooves 9, seven in each row, extending to the sidewalls of platform 2. This allows the sample slide 1 to be placed with one end inside a groove 9, while the other end remains suspended. This is because the sample slide 1 has a relatively large area, with some areas being sample-free and requiring no heating. Therefore, the sample-containing side of the slide can be placed inside the groove 9, while the sample-free side can remain suspended. This reduces the area required for the grooves 9, thus reducing the area of ​​platform 2 and consequently the volume of the tank 3, improving heating efficiency. Furthermore, the suspended portion facilitates the removal of the sample slide 1.

[0056] In the technical solution provided in this embodiment, a transparent window can be provided on the side wall of the tank 3. The transparent window facilitates observation of the internal condition of the tank 3, preventing the inability to understand the internal condition when equipment such as the liquid level sensor 6 malfunctions.

[0057] In the technical solution provided in this embodiment, the heat sink 8 can fully fill the internal space of the slot 3, thereby improving the heat transfer efficiency.

[0058] Among them, see Figure 3 The bottom of the heat sink 8 is spaced a certain distance from the bottom of the groove 3 to reserve space for the heating element 4. (See also...) Figure 4 The heat sink 8 has a first clearance groove at each of its four corners to allow space for the support platform 10, ensuring that the platform 2 can be smoothly installed on the support platform 10. The heat sink 8 has a second clearance groove at its right end to allow space for the liquid level sensor 6, ensuring that the liquid level sensor 6 can detect liquid levels smoothly.

[0059] In the description of this application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0060] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0061] Although preferred embodiments of this application have been described, those skilled in the art, upon learning the basic inventive concept, can make other changes and modifications to these embodiments. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments as well as all changes and modifications falling within the scope of this application.

[0062] Obviously, those skilled in the art can make various modifications and variations to this application without departing from the spirit and scope of this application. Therefore, if such modifications and variations fall within the scope of the claims of this application and their equivalents, this application also intends to include such modifications and variations.

Claims

1. A heating platform for hybridization, characterized in that, Includes platform (2), tank (3), heating element (4); The top of the trough (3) is open, and at least two connecting ports (7) are provided on the side wall of the trough (3). The platform (2) is located at the top opening of the trough (3), and the platform (2) is provided with a groove (9) for placing the sample slide (1), and the bottom of the platform (2) is provided with a heat sink (8). The heating element (4) is disposed inside the tank (3); The heating element (4) is a heating rod; It also includes a temperature sensor (5) for detecting the temperature of the platform (2).

2. The heating platform for hybridization according to claim 1, characterized in that, It also includes a liquid level sensor (6), which is disposed inside the tank (3).

3. The heating platform for hybridization according to claim 2, characterized in that, The platform (2) has a detection hole on its side wall, and the temperature sensor (5) probe is set inside the detection hole.

4. The heating platform for hybridization according to claim 1, characterized in that, The platform (2) is mounted on the tank (3) by bolts.

5. A heating platform for hybridization according to claim 4, characterized in that, The inner wall of the trough (3) is provided with a support platform (10), and the platform (2) is set on the support platform (10) by bolts.

6. A heating platform for hybridization according to claim 5, characterized in that, Each of the four corners of the trough (3) is provided with a support platform (10).

7. A heating platform for hybridization according to claim 1, characterized in that, The platform (2) is provided with multiple grooves (9), which are distributed in a matrix.

Images