Purification kit for nucleic acid extraction

Abstract

The utility model belongs to the technical field of kits, in particular relates to a purification kit for nucleic acid extraction, and provides the following scheme aiming at the problems that reagent leakage is possibly caused and the internal environment of the kit is polluted due to the fact that a reagent tube is extremely easy to shake, incline and even topple over in the transportation, movement or use process of the existing kit. The kit comprises a bottom box, a buffer assembly and a fixing assembly, a cavity is formed in the top of the bottom box, a first layer plate and a second layer plate are arranged in the cavity, a conical hole matched with the diameter of a reagent tube is formed in the top of the first layer plate, and the reagent tube is vertically positioned and supported through the conical hole of the first layer plate and the second layer plate; the reagent tubes are stably fixed one by one by combining a positioning ring, a conical barrel and the like of the fixing assembly, a first air bag and a second air bag of the buffering assembly provide all-directional buffering protection during collision, a connecting piece enhances structural stability, a sponge pad of an upper cover protects the tops of the reagent tubes, the upper cover is conveniently fixed through a lock catch, and nucleic acid extraction accuracy and reliability are ensured.

Description

A purification kit for nucleic acid extraction Technical Field

[0001] This utility model relates to the field of reagent kit technology, and in particular to a purification reagent kit for nucleic acid extraction. Background Technology

[0002] Nucleic acid extraction is a crucial basic operation in many fields such as biomedical research, clinical diagnosis, and genetic engineering. As a key tool for storing and protecting reagent tubes during the nucleic acid extraction process, the performance of purification kits directly affects the efficiency and accuracy of nucleic acid extraction as well as the stability of reagents in the reagent tubes.

[0003] Currently, most nucleic acid extraction and purification kits on the market have relatively simple structures and are significantly inadequate in fixing reagent tubes. Many kits rely solely on simple slots or grooves to hold reagent tubes, lacking an effective fixing mechanism. As a result, during the transportation, movement, or use of the kit, the reagent tubes are prone to shaking, tilting, or even tipping over. This can not only cause reagent leakage and contaminate the internal environment of the kit, affecting subsequent experimental results, but may also lead to reagent tube breakage, damaging precious nucleic acid samples and causing unnecessary losses to scientific research. Summary of the Invention

[0004] The purpose of this invention is to address the shortcomings of existing technologies where reagent tubes are easily shaken, tilted, or even tipped over during the transportation, movement, or use of reagent kits. This can lead to reagent leakage and contamination of the internal environment of the reagent kit. Therefore, this invention proposes a purification reagent kit for nucleic acid extraction.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A purification kit for nucleic acid extraction includes a bottom box, a buffer assembly, and a fixing assembly. The bottom box has a cavity at the top, and a first plate and a second plate are provided inside. The top of the first plate has a conical hole adapted to the diameter of the reagent tube. The second plate is fixedly connected to the bottom of the first plate by a support rod, and the second plate is used to position and support the bottom of multiple reagent tubes.

[0007] The bottom box is hinged to a top cover on one side, and the bottom of the top cover has a cavity with a sponge pad for protecting the reagent tube inside.

[0008] The bottom box has a latch on one side for securing the top cover.

[0009] The buffer assembly is located inside the bottom box. In the event of a collision, the buffer assembly protects the reagent tube. The buffer assembly also includes a connector for fixing the second layer plate inside the bottom box.

[0010] The fixing components are located on the top of the first layer plate, and their number corresponds to the conical holes. The reagent tubes are fixed one by one using the fixing components.

[0011] In one possible design, the buffer assembly includes a first airbag bonded to the cavity of the bottom box. The first airbag is rectangular in shape and its inner wall has a first groove adapted to the first layer plate.

[0012] In one possible design, a second airbag located below the first airbag is bonded inside the cavity of the bottom box. The second airbag is rectangular in shape and its inner wall is provided with a second groove that matches the second layer plate.

[0013] In one possible design, the inner contour dimension of the first groove is larger than the outer contour dimension of the second plate.

[0014] In one possible design, the connector includes a base plate fixed to the inner wall of the second airbag, with a bolt extending through the top of the base plate and threadedly connected to a threaded hole at the bottom of the second layer plate.

[0015] In one possible design, the fixing assembly includes a plurality of guide rods fixedly disposed on the top of the first layer plate. The plurality of guide rods are arranged in a ring around the conical hole. The outer wall of the plurality of guide rods is slidably fitted with the same positioning ring. A conical cylinder that cooperates with the conical hole is fixedly disposed inside the positioning ring. The outer wall of the conical cylinder has a plurality of rectangular openings. The first layer plate is provided with a limiting member corresponding to the positioning ring, and the positioning ring is fixed by the limiting member.

[0016] In one possible design, the limiting member includes a fixing rod fixedly disposed on the top of the first layer plate, and one side of the fixing rod is integrally formed with a wedge block for use with a positioning ring.

[0017] In one possible design, a spring is fitted on the outer wall of the guide rod, and the two ends of the spring are fixedly connected to the positioning ring and the adjacent side of the first layer plate, respectively. A pressing rod is fixedly provided on the top of the positioning ring.

[0018] Beneficial effects:

[0019] In this utility model, a purification reagent kit for nucleic acid extraction has a conical hole at the top of the first plate that matches the diameter of the reagent tube. A fixing assembly is used, in which multiple guide rods are arranged in a ring around the conical hole. A positioning ring is slidably fitted onto the guide rods. A conical cylinder works in conjunction with the conical hole. The positioning ring is fixed by a limiting component. A spring is fitted on the outer wall of the guide rod, with both ends of the spring fixedly connected to the positioning ring and the first plate, respectively. A pressing rod is provided at the top of the positioning ring, which can stably fix each reagent tube individually, preventing the reagent tubes from shaking or tipping during transportation or use, thus ensuring the accuracy and reliability of nucleic acid extraction.

[0020] In this invention, a purification reagent kit for nucleic acid extraction includes a buffer assembly located inside a bottom box. The buffer assembly comprises a first airbag and a second airbag. The first airbag is a rectangular frame attached to the cavity of the bottom box, and its inner wall has a first groove adapted to the first layer plate. The second airbag is located below the first airbag and is also a rectangular frame. Its inner wall has a second groove adapted to the second layer plate. When a collision occurs, the first airbag and the second airbag can respectively provide all-round buffer protection for the reagent tubes on the first layer plate and the second layer plate, effectively reducing the impact force of the collision on the reagent tubes and reducing the risk of damage to the reagent tubes.

[0021] In this invention, a nucleic acid extraction purification reagent kit has a connector that fixes the second layer plate inside the bottom box. The bottom plate is fixed to the inner wall of the second air bladder. Bolts pass through the threaded holes at the bottom of the bottom plate and are threadedly connected to it, which enhances the connection stability between the second layer plate and the bottom box, thereby ensuring the reliability of the positioning support for the bottom of the reagent tube and making the entire reagent kit structure more stable.

[0022] In this invention, the reagent tubes are positioned and supported vertically by the conical holes of the first layer plate and the second layer plate. Combined with the positioning ring and conical cylinder of the fixing component, the reagent tubes are stably fixed one by one. The first and second airbags of the buffer component provide all-round buffer protection during collision. The connector enhances the structural stability. The sponge pad of the top cover protects the top of the reagent tube. The lock makes it easy to fix the top cover. The whole effectively protects the reagent tubes and ensures the accuracy and reliability of nucleic acid extraction. Attached Figure Description

[0023] Figure 1 is a three-dimensional structural diagram of a nucleic acid extraction and purification kit proposed in this utility model;

[0024] Figure 2 is a schematic diagram of the air bladder and layer structure of a nucleic acid extraction and purification kit proposed in this utility model;

[0025] Figure 3 is a schematic cross-sectional view of the first layer of a nucleic acid extraction and purification kit proposed in this invention.

[0026] In the diagram: 1. Base box; 2. Top cover; 3. Sponge pad; 4. First shelf; 5. Positioning ring; 6. Lock; 7. Second shelf; 8. First airbag; 9. First groove; 10. Second airbag; 11. Second groove; 12. Base plate; 13. Bolt; 14. Support rod; 15. Conical cylinder; 16. Rectangular opening; 17. Spring; 18. Conical hole; 19. Guide rod; 20. Pressing rod; 21. Wedge; 22. Fixing rod. Detailed Implementation

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0028] Example 1

[0029] Referring to Figures 1-3, a nucleic acid extraction purification kit includes: a base box 1, which is injection molded from medical-grade polypropylene material, with overall dimensions of 250mm (length) × 180mm (width) × 120mm (height). Its top opening forms a cavity, through which a first layer plate 4 and a second layer plate 7 are detachably mounted. The first layer plate 4 is made of 3mm thick transparent PC material, with six conical holes 18 on its surface, the size of which corresponds to standard nucleic acid reagent tubes, and is available in various sizes to accommodate different specifications of reagent tubes. The second layer plate 7 is connected to the bottom of the first layer plate 4 by four 3mm diameter support rods 14. The support rods 14 are made of nylon material and are evenly spaced in a rectangular array to ensure stable support for the lower reagent tubes.

[0030] The top cover 2 is connected to the bottom box 1 by a stainless steel hinge, and the opening angle can reach 110°. The bottom cavity has a built-in high-density memory foam pad 3 with a foam thickness of 20mm. The latch 6 adopts a push-type spring lock structure, and the surface of the latch is covered with a silicone anti-slip pad to ensure the sealing of the top cover during transportation.

[0031] The cushioning assembly includes a first airbag 8 and a second airbag 10 bonded to the inner wall of the cavity of the bottom box 1 using medical-grade epoxy resin adhesive. The first airbag 8 is located on the upper layer, adopts a rectangular frame structure, has a wall thickness of 1mm, and its inner cavity has a first groove 9 that matches the outer contour of the first layer plate 4, with a groove depth of 5mm. The second airbag 10 is installed on the lower layer, with the same structural dimensions as the first airbag. The second groove 11 is designed to correspond to the outer contour of the second layer plate 7 to ensure that the layer plate remains horizontal when compressed in the vertical direction. At the same time, the inner contour dimension of the first groove 9 is larger than the outer contour dimension of the second layer plate 7, so that the second layer plate 7 can be removed from the two airbags.

[0032] The connector includes a base plate 12 fixed to the inner wall of the second airbag 10 using a high-frequency heat-sealing process. The base plate is made of ABS plastic. The base plate 12 has four pre-drilled M4 threaded holes, corresponding to the mounting holes at the bottom of the second layer plate 7. Stainless steel bolts 13 are used for connection, with the bolt heads embedded in the countersunk holes of the base plate 12 to ensure a smooth surface. This connection structure has been verified by vibration testing and can withstand a 5G acceleration impact without loosening. Simultaneously, through holes corresponding to the bolts 13 are opened at the bottom of the base box 1, allowing the second layer plate 7 to be fixed from the outside.

[0033] The fixing assembly includes four guide rods 19, each with a diameter of Φ3mm and a length of 25mm, mounted in a circular array around each conical hole 18 on the top of the first layer plate 4. The positioning ring 5 is injection molded from polyoxymethylene material, and its inner wall is equipped with linear bearings that mate with the guide rods 19, enabling smooth vertical movement. The conical cylinder 15 and the positioning ring 5 are ultrasonically welded, and its outer wall has four 2mm×8mm rectangular openings 16, allowing it to retract inward under external force.

[0034] Simultaneously, a compression spring 17 is installed around the guide rod 19. The spring wire diameter is 0.8mm, the free length is 20mm, and the pre-compression amount is 3mm. When the reagent tube is inserted into the conical hole 18, the conical cylinder 15 can cooperate with the conical hole 18 under the downward pressing force to automatically clamp the tube body and achieve radial fixation. A T-shaped pressing rod 20 is integrated at the top of the positioning ring 5 for easy one-handed operation and release.

[0035] The fixing rod 22 is made of a 1mm x 4mm stainless steel sheet, which can deform under external force and automatically return to its original position after the external force is removed. It is fixed to the surface of the first layer plate 4 by welding. A wedge 21 is integrally formed on one side of the fixing rod 22. The wedge 21 cooperates with the positioning ring 5 to fix the positioning ring 5 after it is pressed into place. The contact surface is designed as a 15° slope. When the positioning ring 5 is pressed down, it automatically locks and provides a holding force of not less than 5N.

[0036] In use, unlock the latch 6, open the top cover 2, and then insert the reagent tube into the corresponding positioning ring 5 until the bottom of the reagent tube touches the second layer plate 7. Then press down the pressing rod 20. The pressing rod 20 moves down and drives the positioning ring 5 to move down. The positioning ring 5 moves down and drives the conical cylinder 15 to move down. When the conical cylinder 15 moves into the conical hole 18, the conical surface of the conical hole 18 can drive the conical cylinder 15 to contract inward, thereby clamping the reagent tube.

[0037] When the positioning ring 5 touches the wedge block 21, the inclined surface of the wedge block 21 can drive the fixing rod 22 to tilt and deform until the positioning ring 5 moves to the bottom of the wedge block 21. At this time, the fixing rod 22 can reset under its own elasticity, so that the wedge block 21 is stuck on the top of the positioning ring 5, and the positioning ring 5 can compress the spring 17 during the downward movement.

[0038] When it is necessary to remove the fixation of the reagent tube, simply move the fixing rod 22 outward to tilt it. At this time, the wedge 21 moves away from the positioning ring 5, and the positioning ring 5 can be reset and moved under the force of multiple springs 17, and drive the conical cylinder 15 to disengage from the conical hole 18. The conical cylinder 15 can be reset and release the fixation of the reagent tube under its own elasticity.

[0039] When a collision occurs, the first plate 4 and the second plate 7 can buffer the impact under the force of the first airbag 8 and the second airbag 10.

[0040] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A purification kit for nucleic acid extraction, comprising a base box (1), a buffer assembly, and a fixation assembly, characterized in that, The bottom box (1) has a cavity at the top, inside which are a first layer plate (4) and a second layer plate (7). The top of the first layer plate (4) has a conical hole (18) that matches the diameter of the reagent tube. The second layer plate (7) is fixedly connected to the bottom of the first layer plate (4) by a support rod (14), and the second layer plate (7) is used to position and support the bottom of multiple reagent tubes. A top cover (2) is hinged to one side of the bottom box (1). The bottom of the top cover (2) has a cavity, inside which are... There is a sponge pad (3) for protecting the reagent tubes; a latch (6) for fixing the top cover (2) is provided on one side of the bottom box (1); the buffer assembly is set inside the bottom box (1) and the reagent tubes are protected by the buffer assembly when a collision occurs; the buffer assembly also includes a connector for fixing the second layer plate (7) inside the bottom box (1); the fixing assembly is set on the top of the first layer plate (4) and its number corresponds to the conical holes (18); the fixing assembly is used to fix the reagent tubes one by one.

2. The nucleic acid extraction and purification kit according to claim 1, characterized in that, The buffer assembly includes a first airbag (8) bonded to the cavity of the bottom box (1). The first airbag (8) is in the shape of a rectangular frame, and its inner wall is provided with a first groove (9) that is adapted to the first layer plate (4).

3. The nucleic acid extraction and purification kit according to claim 2, characterized in that, The cavity of the bottom box (1) is bonded with a second airbag (10) located below the first airbag (8). The second airbag (10) is rectangular frame-shaped, and its inner wall is provided with a second groove (11) that is adapted to the second layer plate (7).

4. The nucleic acid extraction and purification kit according to claim 3, characterized in that, The inner contour dimension of the first groove (9) is larger than the outer contour dimension of the second layer plate (7).

5. A nucleic acid extraction and purification kit according to claim 3, characterized in that, The connector includes a base plate (12) fixed to the inner wall of the second airbag (10), and a bolt (13) is provided through the top of the base plate (12), which is threadedly connected to the threaded hole at the bottom of the second layer plate (7).

6. The nucleic acid extraction and purification kit according to claim 1, characterized in that, The fixing assembly includes multiple guide rods (19) fixedly disposed on the top of the first layer plate (4). The multiple guide rods (19) are arranged in a ring around the conical hole (18). The outer wall of the multiple guide rods (19) is slidably fitted with the same positioning ring (5). A conical cylinder (15) that cooperates with the conical hole (18) is fixedly disposed inside the positioning ring (5). The outer wall of the conical cylinder (15) is provided with multiple rectangular openings (16). The first layer plate (4) is provided with a limiting member corresponding to the positioning ring (5). The positioning ring (5) is fixed by the limiting member.

7. A nucleic acid extraction and purification kit according to claim 6, characterized in that, The limiting component includes a fixing rod (22) fixedly installed on the top of the first layer plate (4), and a wedge (21) integrally formed on one side of the fixing rod (22) for use with the positioning ring (5).

8. A nucleic acid extraction and purification kit according to claim 6, characterized in that, The outer wall of the guide rod (19) is fitted with a spring (17), and the two ends of the spring (17) are fixedly connected to the positioning ring (5) and the first layer plate (4) respectively. The top of the positioning ring (5) is fixedly fitted with a pressing rod (20).

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