A nucleic acid synthesis 384 well plate for improved synthesis rate

Abstract

The utility model discloses a nucleic acid synthesis 384 -hole plate of improving synthetic rate, including 384 -hole plate main part and hole plate top cover, the bottom equal interval of 384 -hole plate main part is fixed with a plurality of synthesis pipe, the bottom middle position of 384 -hole plate main part is fixed with mutually perpendicular longitudinal stiffening and transverse stiffening, all be equipped with the communicating port on longitudinal stiffening and transverse stiffening, the top of 384 -hole plate main part is fixed with the multiple anti -spill along of convex, the bottom middle position of 384 -hole plate main part is fixed with mutually perpendicular longitudinal stiffening and transverse stiffening, can strengthen the intensity of 384 -hole plate main part through longitudinal stiffening and transverse stiffening, makes 384 -hole plate main part not easy to bend, to avoid longitudinal stiffening and transverse stiffening divide the synthesis pipe of 384 -hole plate main part bottom into four areas of not intercommunication, and then reduce when with nucleic acid synthesizer cooperation, the synthesis efficiency and quality of nucleic acid.

Description

Technical Field

[0001] This invention relates to the field of nucleic acid synthesis plate technology, specifically a 384-well plate for improving the synthesis rate of nucleic acid. Background Technology

[0002] PCR plates can increase the efficiency of nucleic acid synthesis. As the core experimental equipment for nucleic acid amplification reactions, PCR plates are primarily made of polypropylene (PP), with some products using polycarbonate (PC) composite materials. They feature high chemical stability, ultra-thin walls, and extremely fast temperature conduction. These characteristics allow PCR plates to rapidly and evenly transfer heat, thereby improving the efficiency of nucleic acid amplification. Furthermore, the transparent design of PCR plates facilitates sample observation, further optimizing the experimental process. Specific specifications of PCR plates include single tubes, 8-well strips, 96-well PCR plates, and 384-well PCR plates, which can meet different experimental needs. For example, 96-well and 384-well PCR plates are suitable for large-scale sample processing and high-throughput screening, while single tubes and 8-well strips are suitable for small-scale experiments or specific experimental requirements.

[0003] Due to their size and specifications, existing 384PCR plates have limited inherent strength, making them prone to bending under external pressure. This can easily lead to leakage of the synthesis tubes from the 384PCR plate. Utility Model Content

[0004] The purpose of this invention is to provide a 384-well plate for nucleic acid synthesis that improves the synthesis rate, thereby solving the problems in the prior art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a 384-well plate for improving the synthesis rate of nucleic acid, comprising a 384-well plate body and a plate top cover, wherein multiple synthesis tubes are fixed at equal intervals at the bottom of the 384-well plate body, and a longitudinal reinforcing plate and a transverse reinforcing plate perpendicular to each other are fixed at the middle position of the bottom of the 384-well plate body, and both the longitudinal reinforcing plate and the transverse reinforcing plate have communication openings, and multiple protruding anti-overflow edges are fixed at the top of the 384-well plate body, wherein each anti-overflow edge corresponds to a synthesis tube, and a sheet-like anti-splash edge is fixed on one side of the top of each anti-overflow edge.

[0006] Preferably, a protective frame is installed below the main body of the 384-hole plate.

[0007] Preferably, the top of the protective frame is provided with a perforated plate main support platform, and the middle position of the perforated plate main support platform is provided with a slot that cooperates with the longitudinal reinforcing plate and the transverse reinforcing plate.

[0008] Preferably, the top edge of the 384-hole plate body is provided with a protruding top cover mating edge, and the middle of the side of the top cover of the hole plate is provided with a recessed auxiliary groove.

[0009] Compared with the prior art, the beneficial effects of this utility model are as follows: The bottom middle position of the 384-well plate body of this utility model is fixed with mutually perpendicular longitudinal reinforcing plates and transverse reinforcing plates. The longitudinal and transverse reinforcing plates can enhance the strength of the 384-well plate body, making the 384-well plate body less prone to bending. In order to avoid the longitudinal and transverse reinforcing plates dividing the synthesis tube at the bottom of the 384-well plate body into four non-connected areas, thereby reducing the synthesis efficiency and quality of nucleic acid when used with a nucleic acid synthesizer, both the longitudinal and transverse reinforcing plates are provided with connecting ports. In order to prevent liquid from overflowing when adding liquid to the synthesis tube, multiple protruding anti-overflow edges are provided above the synthesis tube. In order to prevent liquid from splashing due to excessive liquid impact force when adding liquid, a sheet-like anti-splash edge is fixed on one side of the top of the anti-overflow edge. When adding liquid, the injection head can be inserted into the synthesis tube away from the anti-splash edge and tilted at a certain angle so that the water droplets generated by the impact are concentrated on the side close to the anti-overflow edge.

[0010] A protective frame is installed at the bottom of the 384-hole plate body, which can protect the sides of the 384-hole plate body. Attached Figure Description

[0011] The accompanying drawings are provided to further illustrate the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention, but do not constitute a limitation thereof. In the drawings:

[0012] Figure 1 This is a schematic diagram of the top structure of the 384-hole plate body of this utility model;

[0013] Figure 2 This is a schematic diagram of the bottom structure of the 384-hole plate body of this utility model;

[0014] Figure 3 This is a schematic diagram of the combined structure of the 384-hole plate body, the top cover of the hole plate, and the protective frame of this utility model;

[0015] Figure 4 This is a schematic diagram of the protective frame of this utility model.

[0016] In the figure: 1. 384 orifice plate body; 2. Composite pipe; 3. Longitudinal reinforcing plate; 4. Top cover mating edge; 5. Overflow edge; 6. Splash edge; 7. Transverse reinforcing plate; 8. Connecting port; 9. Orifice plate top cover; 10. Auxiliary groove; 11. Protective frame; 12. Orifice plate body support platform; 13. Slot. Detailed Implementation

[0017] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this utility model, not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model. Therefore, the following detailed description of the embodiments of this utility model provided in the accompanying drawings is not intended to limit the scope of the claimed utility model, but merely represents selected embodiments of this utility model. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model.

[0018] Please see Figure 1-4 In this embodiment of the invention, a 384-well plate for improving the synthesis rate of nucleic acid includes a 384-well plate body 1 and a plate top cover 9. Multiple synthesis tubes 2 are fixed at equal intervals at the bottom of the 384-well plate body 1. Vertically perpendicular longitudinal reinforcing plates 3 and lateral reinforcing plates 7 are fixed at the middle of the bottom of the 384-well plate body 1. Both the vertically perpendicular longitudinal reinforcing plates 3 and lateral reinforcing plates 7 have connecting openings 8. Multiple protruding anti-overflow edges 5 are fixed at the top of the 384-well plate body 1, each corresponding to a synthesis tube 2. A sheet-like anti-splash edge 6 is fixed to one side of the top of each anti-overflow edge 5. The vertically perpendicular longitudinal reinforcing plates 3 and lateral reinforcing plates 7 at the middle of the bottom of the 384-well plate body 1 enhance the strength of the 384-well plate body 1, thereby improving the synthesis rate. The 384-well plate body 1 is not easily bent. To prevent the longitudinal reinforcing plate 3 and the transverse reinforcing plate 7 from dividing the synthesis tube 2 at the bottom of the 384-well plate body 1 into four non-connected areas, thereby reducing the efficiency and quality of nucleic acid synthesis when used with a nucleic acid synthesizer, both the longitudinal reinforcing plate 3 and the transverse reinforcing plate 7 are provided with connecting ports 8. To prevent liquid from overflowing when adding liquid to the synthesis tube 2, multiple protruding anti-overflow edges 5 are provided above the synthesis tube 2. To prevent liquid from splashing due to excessive liquid impact during liquid addition, a sheet-like anti-splash edge 6 is fixed to one side of the top of the anti-overflow edge 5. When adding liquid, the injection head can be inserted into the synthesis tube 2 away from the side of the anti-splash edge 6 and tilted at a certain angle so that the water droplets generated by the impact are concentrated on the side close to the anti-overflow edge 5.

[0019] A protective frame 11 is installed below the main body 1 of the 384-hole plate. The side of the main body 1 of the 384-hole plate can be protected by the protective frame 11. The top of the protective frame 11 is provided with a support platform 12 for the main body of the plate. A slot 13 that cooperates with the longitudinal reinforcing plate 3 and the transverse reinforcing plate 7 is opened in the middle of the support platform 12.

[0020] The top edge of the 384-hole plate body 1 is provided with a protruding top cover fitting edge 4, and the middle of the side of the top cover 9 of the hole plate is provided with a recessed auxiliary groove 10.

[0021] The working principle of this utility model is as follows: At the bottom center of the 384-well plate body 1, there are vertically perpendicular longitudinal reinforcing plates 3 and transverse reinforcing plates 7. The longitudinal reinforcing plates 3 and transverse reinforcing plates 7 can enhance the strength of the 384-well plate body 1, making it less prone to bending. To prevent the longitudinal reinforcing plates 3 and transverse reinforcing plates 7 from dividing the synthesis tube 2 at the bottom of the 384-well plate body 1 into four non-connected areas, thereby reducing the synthesis efficiency and quality of nucleic acid when used with a nucleic acid synthesizer, both the longitudinal reinforcing plates 3 and transverse reinforcing plates 7 are provided with connecting ports 8. To prevent liquid from overflowing when adding liquid to the synthesis tube 2, multiple protruding anti-overflow edges 5 are provided above the synthesis tube 2. To prevent liquid from splashing due to excessive liquid impact force when adding liquid, a sheet-like anti-splash edge 6 is fixed on one side of the top of the anti-overflow edge 5. When adding liquid, the injection head can be inserted into the synthesis tube 2 away from the anti-splash edge 6 and tilted at a certain angle so that the water droplets generated by the impact are concentrated on the side close to the anti-overflow edge 5.

[0022] A protective frame 11 is installed at the bottom of the 384-hole plate body 1, which can protect the side of the 384-hole plate body 1.

[0023] Finally, it should be noted that the above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A 384-well plate for improving the synthesis rate of nucleic acid, comprising a 384-well plate body (1) and a plate top cover (9), wherein a plurality of synthesis tubes (2) are fixed at equal intervals at the bottom of the 384-well plate body (1), characterized in that: The bottom middle position of the 384-hole plate body (1) is fixed with a vertically perpendicular longitudinal reinforcing plate (3) and a transverse reinforcing plate (7). Both the longitudinal reinforcing plate (3) and the transverse reinforcing plate (7) are provided with a communication port (8). The top of the 384-hole plate body (1) is fixed with a plurality of protruding anti-overflow edges (5). The anti-overflow edges (5) correspond one-to-one with the synthetic pipe (2). The top side of the anti-overflow edge (5) is fixed with a sheet-like anti-splash edge (6).

2. The 384-well plate for improving the synthesis rate of nucleic acid according to claim 1, characterized in that: A protective frame (11) is installed below the main body (1) of the 384-hole plate.

3. The 384-well plate for improving the synthesis rate of nucleic acid according to claim 2, characterized in that: The top of the protective frame (11) is provided with a perforated plate main support platform (12), and a slot (13) is provided in the middle of the perforated plate main support platform (12) to cooperate with the longitudinal reinforcing plate (3) and the transverse reinforcing plate (7).

4. The 384-well plate for improving the synthesis rate of nucleic acid according to claim 1, characterized in that: The top edge of the 384-hole plate body (1) is provided with a protruding top cover fitting edge (4), and the middle side of the top cover (9) of the hole plate is provided with a recessed auxiliary groove (10).

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