Plant cell exosome extraction device

Abstract

The utility model relates to the technical field of plant cell exosome centrifugal extraction, especially to a kind of plant cell exosome extraction device;It is separated by the combination mode of oscillation and centrifugation to realize plant cell and exosome, shorten separation length, further improve separation effect and separation efficiency;Including rack, fixedly installed drive motor on rack, centrifugal disc installed on the output end of drive motor upper portion, multiple sets of vibration centrifugal components are installed at the edge of centrifugal disc and are used to drive the vibration of multiple sets of vibration centrifugal components vibration driving part, and vibration centrifugal component includes test tube, ball seat fixedly installed on centrifugal disc and sphere rotationally installed on ball seat, through slot hole that is engaged with the outer wall of test tube is equipped on sphere, test tube is placed in through slot hole, and vibration driving part includes ring frame and multiple support rod parts, and ring frame is fixedly connected with rack by multiple support rod parts, and multiple evenly distributed arc grooves are equipped on ring frame.

Description

Technical Field

[0001] This utility model relates to the technical field of centrifugal extraction of plant cell exosomes, and in particular to a plant cell exosome extraction device. Background Technology

[0002] Plant exosomes, in a narrow sense, refer to extracellular vesicles (EVs) of a specified size range secreted by plant cells. The extraction methods for plant exosomes are similar to those for other exosomes, and traditional separation methods such as ultracentrifugation and immunoaffinity capture can be used; among these, ultracentrifugation is the most widely used.

[0003] For example, Chinese utility model patent application CN220991290U discloses a plant cell exosome extraction device, including an extraction cylinder with a turntable and a drive mechanism installed inside. A test tube rack is attached to the turntable, and multiple sample test tubes containing a plant cell mixture are placed on the rack. A detachable sealing cover is installed on the test tube rack to seal the mouths of the multiple sample test tubes. This device prevents leakage of the mixture during ultracentrifugation, is simple and convenient to operate, saves time and effort, and thus improves practical work efficiency.

[0004] However, the above-mentioned device still has the following drawbacks: the extraction time of plant cell exosomes by centrifugation alone is long, and the efficiency of separating extracellular vesicles from plant cells needs to be further improved. Utility Model Content

[0005] To address the shortcomings of existing technologies, this invention provides a plant cell exosome extraction device that uses a combination of oscillation and centrifugation to separate plant cells and exosomes, thereby shortening the separation time and further improving the separation effect and efficiency.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a plant cell exosome extraction device, comprising a frame, a drive motor fixedly mounted on the frame, a centrifuge disc mounted on the upper output end of the drive motor, multiple sets of vibrating centrifuge components mounted on the edge of the centrifuge disc, and a vibration drive component for driving the multiple sets of vibrating centrifuge components to vibrate. Each vibrating centrifuge component includes a test tube, a ball seat fixedly mounted on the centrifuge disc, and a sphere rotatably mounted on the ball seat. The sphere has a through-hole that fits into the outer wall of the test tube, and the test tube is placed in the through-hole. The vibration drive component includes a ring frame and multiple support rods. The ring frame is fixedly connected to the frame through the multiple support rods, and the ring frame has multiple evenly distributed arc-shaped slots. The ring frame is located in the lower middle part of the test tube. It should be noted that, to prevent the test tube from breaking, the test tube can be made of transparent acrylic material.

[0007] Preferably, a flexible pad is bonded to the inner wall of the ring frame and the inner wall of the arc-shaped groove; further, the flexible pad is preferably a rubber pad or a silicone pad, or other equivalent components with flexible anti-collision and friction effects can also be used.

[0008] Preferably, the support rod is a telescopic rod; further, the telescopic rod is preferably an electric telescopic rod.

[0009] Preferably, the bottom of the sphere is provided with a limiting plate whose outer diameter is larger than the inner diameter of the through slot, and a gap is left between the limiting plate and the bottom of the ball seat to allow the sphere to rotate.

[0010] Preferably, a malleable limiting ring is provided at the inner wall of the through slot; further, the malleable limiting ring is preferably a rubber ring, but other equivalent parts with elastic locking and limiting effects can also be used.

[0011] Preferably, the top of the test tube has a flared section with an outer diameter larger than the inner diameter of the through slot.

[0012] Preferably, the drive motor is a dual-shaft motor, and a speed regulating disk is fixedly installed at the bottom output end of the dual-shaft motor. Electromagnets corresponding to the speed regulating disk are installed on the frame, and the speed regulating disk is made of magnetic material. Further, the electromagnets are preferably U-shaped, and the magnetic attraction points of the U-shaped electromagnets are located at the upper and lower parts of the speed regulating disk, respectively. The speed regulating disk is made of iron or other equivalent materials with magnetic attraction effect.

[0013] Preferably, it also includes an outer casing, the outer casing having a mounting cavity, and the frame being fixedly installed in the mounting cavity; further, the outer casing has a control panel, and the frame has a control circuit board installed on it, the control panel being electrically connected to the electromagnet and the drive motor through the control circuit board.

[0014] Compared with the prior art, this utility model provides a plant cell exosome extraction device with the following beneficial effects: This plant cell exosome extraction device, by placing the extract to be extracted into each test tube, drives a motor to rotate a centrifuge disc. The upper part of the test tube is limited by a sphere. Under the action of centrifugal force, the bottom of the test tube moves away from the center of the centrifuge disc and contacts the inner wall of the ring holder. The rotating test tube frequently contacts and separates from the arc-shaped groove on the ring holder. The extract in the test tube is subjected to both centrifugal force and oscillation force. Under the action of centrifugation and oscillation, plant cells and exosomes are separated through a combination of oscillation and centrifugation, shortening the separation time and further improving the separation effect and efficiency. Attached Figure Description

[0015] Figure 1 This is a three-dimensional structural schematic diagram of the present invention;

[0016] Figure 2 This is a three-dimensional structural diagram of the present invention in its shell-less state;

[0017] Figure 3 This is a top view of the shell-less planar structure of this utility model;

[0018] Figure 4 This is the utility model Figure 3 Schematic diagram of the cross-sectional structure at point AA;

[0019] Figure 5 This is the utility model Figure 4 Schematic diagram of the cross-sectional structure at point BB;

[0020] The following are labels in the attached diagram: 1. Frame; 2. Drive motor; 3. Centrifuge disc; 4. Test tube; 5. Ball seat; 6. Sphere; 7. Through slot; 8. Ring frame; 9. Arc-shaped slot; 10. Soft pad; 11. Telescopic rod; 12. Limiting plate; 13. Molded limiting ring; 14. Flared part; 15. Speed ​​control disc; 16. Electromagnet; 17. Outer shell. Detailed Implementation

[0021] To enable those skilled in the art to better understand the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings.

[0022] As described in the background art, a plant cell exosome extraction device is used; the extraction time of plant cell exosomes by centrifugation alone is long, and the efficiency of separating extracellular vesicles from plant cells needs to be further improved.

[0023] To address this technical problem, this invention provides a plant cell exosome extraction device, which is used for the separation of plant cell exosomes.

[0024] It should be noted that, unless otherwise specified, the embodiments and features and technical solutions in the present invention can be combined with each other.

[0025] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0026] Example 1

[0027] Please refer to Figure 2-5A plant cell exosome extraction device includes: a frame 1, a drive motor 2 fixedly mounted on the frame 1, a centrifuge disc 3 mounted on the upper output end of the drive motor 2, multiple sets of vibrating centrifuge components mounted on the edge of the centrifuge disc 3, and a vibration drive for driving the multiple sets of vibrating centrifuge components to vibrate. The vibrating centrifuge components include test tubes 4, ball seats 5 fixedly mounted on the centrifuge disc 3, and spheres 6 rotatably mounted on the ball seats 5. The spheres 6 have through slots 7 that fit into the outer wall of the test tubes 4, and the test tubes 4 are placed in the through slots 7. The vibration drive includes a ring frame 8 and multiple support rods. The ring frame 8 is fixedly connected to the frame 1 through the multiple support rods. The ring frame 8 has multiple evenly distributed arc-shaped slots 9. The ring frame 8 is located in the lower middle part of the test tubes 4. It should be noted that, in order to prevent the test tubes 4 from breaking, the test tubes 4 can be made of transparent acrylic material. In order to prevent the extract from splashing outwards, the amount of extract in the test tubes 4 should not exceed 1 / 3 of the capacity of the test tubes 4.

[0028] For details, please refer to Figure 5 A flexible pad 10 is bonded to the inner wall of the ring frame 8 and the inner wall of the arc-shaped groove 9; furthermore, the flexible pad 10 is preferably a rubber pad or a silicone pad, but other equivalent parts with flexible anti-collision effect and friction effect can also be used.

[0029] For details, please refer to Figure 4 A malleable limiting ring 13 is provided at the inner wall of the through slot 7; furthermore, the malleable limiting ring 13 is preferably a rubber ring, but other equivalent parts with elastic locking and limiting effects can also be used.

[0030] For details, please refer to Figure 2 The top of the test tube 4 is provided with a flared part 14 whose outer diameter is larger than the inner diameter of the through slot 7.

[0031] The plant cell exosome extraction device provided in this embodiment can mitigate the impact force between the test tube 4, the ring 8, and the arc-shaped groove 9 through the soft pad 10, effectively reducing impact noise. The soft pad 10 can also provide tangential friction to the outer wall of the test tube 4. Under the action of friction, the test tube 4 drives the sphere 6 to rotate in the ball seat 5. The test tube 4 rotates on its own while following the rotation of the centrifuge plate 3, providing multi-directional centrifugal force to the extract in the test tube 4, thereby further improving the separation effect and efficiency of plant cells and exosomes. The plastic limiting ring 13 can tightly fix the test tube 4 in the through hole 7 of the sphere 6, avoiding relative sliding between the sphere 6 and the test tube 4. The flared part 14 at the top of the test tube 4 can facilitate the addition of extract into the test tube 4, while preventing the test tube 4 from sliding down from the through hole of the sphere 6.

[0032] For details, please refer to Figure 4The drive motor 2 is a dual-shaft motor, and a speed regulating disk 15 is fixedly installed at the bottom output end of the dual-shaft motor. An electromagnet 16 corresponding to the speed regulating disk 15 is installed on the frame 1. The speed regulating disk 15 is made of magnetic material. Furthermore, the electromagnet 16 is preferably U-shaped, and the magnetic attraction points of the U-shaped electromagnet 16 are located at the upper and lower parts of the speed regulating disk 15, respectively. The speed regulating disk 15 is made of iron or other equivalent materials with magnetic attraction effect. The current entering the electromagnet 16 can be controlled by an external current controller to adjust the electromagnetic attraction force of the U-shaped electromagnet 16, so as to adjust the speed of the centrifugal disk 3.

[0033] For details, please refer to Figure 2 The support rod is a telescopic rod 11; furthermore, the telescopic rod 11 can preferably be an electric telescopic rod 11; the telescopic rod 11 can also be a hydraulic rod or a pneumatic rod, and the extension or shortening principle of the telescopic rod 11 adopts the existing technology, which will not be further elaborated or limited here.

[0034] For details, please refer to Figure 1 It also includes an outer casing 17, which has a mounting cavity inside, and the frame 1 is fixedly installed in the mounting cavity; furthermore, the outer casing 17 is provided with a control panel, and the frame 1 is equipped with a control circuit board, which is electrically connected to the electromagnet 16 and the drive motor 2 through the control circuit board.

[0035] For details, please refer to Figure 4 The bottom of the sphere 6 is provided with a limiting plate 12 whose outer diameter is larger than the inner diameter of the through slot 7, and a gap is left between the limiting plate 12 and the bottom of the ball seat 5 to allow the sphere 6 to rotate.

[0036] The plant cell exosome extraction device provided in this embodiment, by activating the electromagnet 16, generates magnetic resistance on the speed regulating disk 15, thereby adjusting the speed of the drive motor 2 to meet different speed requirements; when only the extract in the test tube 4 is centrifuged at high speed, the output end of the telescopic rod 11 shortens, the ring frame 8 moves down, and the ring frame 8 can disengage from the test tube 4, avoiding interference with the high-speed rotation of the test tube 4 along with the centrifuge disk 3, thus ensuring the subsequent high-speed centrifugation rotation of the test tube 4; the outer shell 17 can prevent the operator from contacting the rotating parts, reducing mechanical injury, and at the same time, the centrifugation speed, whether to vibrate, etc. can be appropriately controlled as needed through the control panel, etc. The limiting plate 12 can prevent the ball 6 from detaching from the ball seat 5, and at the same time can limit the rotation angle of the ball 6, avoiding excessive rotation of the ball 6 causing the bottom of the test tube 4 to shift excessively outwards from the centrifuge disk 3 and contact the inner wall of the mounting cavity of the outer shell 17.

[0037] The process of using the plant cell exosome extraction device provided by this utility model is as follows: The extract is injected into test tube 4, and test tube 4 is inserted into the through-hole 7 on the sphere 6. The extract is then placed into each test tube 4. The drive motor 2 rotates the centrifuge disc 3. The upper part of the test tube 4 is limited by the sphere 6. Under the action of centrifugal force, the bottom of the test tube 4 moves away from the center of the centrifuge disc 3 and contacts the inner wall of the ring frame 8. The rotating test tube 4 frequently contacts and separates from the arc-shaped groove 9 on the ring frame 8. Simultaneously, the test tube 4 rotates under the friction of the soft pad 10, and the extract inside the test tube 4... The extract is subjected to both centrifugal and oscillatory forces. Under the combined effects of centrifugation and oscillation, plant cells and exosomes are separated. During this process, the centrifuge disc 3 can move at medium to high speeds. Subsequently, the telescopic rod 11 moves the ring frame 8 downwards until the top of the ring frame 8 is lower than the bottom of the test tube 4. The speed of the drive motor 2 increases, and the centrifuge disc 3 drives the test tube 4 to move at ultra-high speeds to further promote the separation of plant cells and exosomes. When the plant cells and exosomes reach the set separation time, the device stops operating, and the test tube 4 can be removed for subsequent separation operations.

[0038] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between them; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

Claims

1. A plant cell exosome extraction device, characterized by, The device includes a frame (1), a drive motor (2) fixedly mounted on the frame (1), a centrifuge disc (3) mounted on the upper output end of the drive motor (2), multiple sets of vibrating centrifuge components mounted on the edge of the centrifuge disc (3), and a vibration drive for driving the multiple sets of vibrating centrifuge components to vibrate. The vibrating centrifuge components include a test tube (4), a ball seat (5) fixedly mounted on the centrifuge disc (3), and a ball (6) rotatably mounted on the ball seat (5). The ball (6) is provided with a through slot (7) that fits into the outer wall of the test tube (4). The test tube (4) is placed in the through slot (7). The vibration drive includes a ring frame (8) and multiple support rods. The ring frame (8) is fixedly connected to the frame (1) through multiple support rods. The ring frame (8) is provided with multiple evenly distributed arc-shaped slots (9).

2. The plant exosome extraction device of claim 1, wherein, A soft pad (10) is bonded to the inner wall of the ring frame (8) and the inner wall of the arc-shaped groove (9).

3. The plant cell exosome extraction device according to claim 2, characterized in that, The support rod is a telescopic rod (11).

4. The plant exosome extraction device of claim 1, wherein, The bottom of the sphere (6) is provided with a limiting plate (12) with an outer diameter larger than the inner diameter of the through slot (7), and a gap is left between the limiting plate (12) and the bottom of the ball seat (5) to allow the sphere (6) to rotate.

5. The plant exosome extraction device of claim 4, wherein, A malleable limiting ring (13) is provided on the inner wall of the through slot (7).

6. The plant exosome extraction device of claim 1, wherein, The test tube (4) has a flared section (14) at the top with an outer diameter larger than the inner diameter of the through slot (7).

7. The plant exosome extraction device of claim 1 or 3, wherein, The drive motor (2) is a dual-shaft motor. A speed control plate (15) is fixedly installed at the bottom output end of the dual-shaft motor. An electromagnet (16) corresponding to the speed control plate (15) is installed on the frame (1). The speed control plate (15) is made of magnetic material.

8. The plant exosome extraction device of claim 1, wherein, It also includes an outer shell (17), which has an installation cavity inside, and the frame (1) is fixedly installed in the installation cavity.

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