Flow cytometry signal collection module and collection device

Abstract

The utility model discloses a flow cytometer signal collection module and collection device. Flow cytometer signal collection module includes casing, optical mounting seat, light guide component, optical fiber subassembly and detection component, is equipped with a plurality of insertion slot and the light guide groove that links and communicates with a plurality of insertion slot on optical mounting seat, and the light guide groove has the opening, the light guide component includes a plurality of light guide support and a plurality of light guide lenses, and a plurality of light guide lenses are arranged in the light guide groove along the lateral direction of casing, the optical fiber subassembly is used for conducting the excitation fluorescence to the light guide lens of the most front end along the lateral direction of casing, the detection component is set to correspond opening, and the insertion slot is equipped with multiple groups, and multiple groups insertion slot are sequentially arranged along the longitudinal direction of casing, the light guide component is equipped with multiple sets, and each set of light guide component is set to correspond a group of insertion slot. The utility model makes the structure of entire flow cytometer signal collection module more compact, and the volume is smaller, and the detection efficiency is higher, and the detection result is more reliable.

Description

Technical Field

[0001] This utility model relates to the technical field of biomedical devices, specifically to a flow cytometer signal collection module and collection device. Background Technology

[0002] Flow cytometry is a core tool for high-throughput analysis of single cells or biological particles using laser excitation and photoelectric detection technologies. Its core function is to achieve multi-dimensional analysis of cell phenotype, function, and molecular characteristics. The core functional modules of a flow cytometer include four major systems: laser excitation, optical detection, fluid flow control, and data processing. Traditional single-laser multi-channel optical detection systems, i.e., signal collection modules, use multiple optical systems of different wavelengths to simultaneously acquire excitation fluorescence at various wavelengths. Each channel is independent, occupies a large space, and the complex structure of multiple channels makes the entire signal collection module quite large. Utility Model Content

[0003] To address the aforementioned technical problems, the main objective of this utility model is to provide a flow cytometer signal collection module and collection device, aiming to solve the problem that the traditional flow cytometer signal collection module has a complex structure, resulting in a large overall size of the signal collection module.

[0004] To achieve the above objectives, this utility model proposes a flow cytometer signal acquisition module, comprising:

[0005] case;

[0006] An optical mounting base is disposed within the housing. The optical mounting base has a plurality of insertion slots arranged at intervals along the transverse direction of the housing, and a light guide groove that communicates with all of the plurality of insertion slots. The light guide groove has an opening that penetrates the rear side of the optical mounting base.

[0007] The light guide component includes multiple light guide brackets and multiple light guide lenses that are correspondingly disposed on the multiple light guide brackets. The multiple light guide brackets are inserted into the insertion slots, and the multiple light guide lenses are arranged in the light guide slots at lateral intervals along the housing.

[0008] An optical fiber assembly is disposed within the housing and extends at least partially out of the housing. The optical fiber assembly is used to conduct the excited fluorescence toward the light guide lens located at the foremost end along the transverse direction of the housing, so that the light is reflected by the light guide lens and then conducted along the direction of the light guide groove to be emitted from the opening.

[0009] The detection component is disposed inside the housing and is positioned corresponding to the opening;

[0010] Multiple insertion slots are arranged at intervals along the transverse direction of the housing to form a set of insertion slots. Multiple sets of insertion slots are arranged sequentially along the longitudinal direction of the housing. Multiple sets of light guide components are provided, and each set of light guide components corresponds to a set of insertion slots.

[0011] Optionally, the dimensions of the multiple insertion slots within the same group are set differently.

[0012] Optionally, the optical mounting base has two receiving cavities extending longitudinally along the housing, the two receiving cavities are spaced apart laterally along the housing, and each receiving cavity has a plurality of partition protrusions protruding from its sidewall, the space between each pair of adjacent partition protrusions forming a insertion groove.

[0013] Optionally, the optical mounting base includes:

[0014] The first seat and the second seat are detachably connected. The first seat has a first half-groove, and the second seat has a second half-groove that is opposite to the first half-groove.

[0015] A partition plate is disposed between the first base and the second base. The two sides of the partition plate respectively block the open sides of the first half-groove and the second half-groove to form two receiving cavities. Multiple partition protrusions are provided on the side wall of each receiving cavity. The space between each pair of adjacent partition protrusions forms a insertion groove.

[0016] Optionally, a first insertion groove is formed between the first seat and the partition plate, and a second insertion groove is formed between the second seat and the partition plate;

[0017] The light guide lens is provided in multiple ways, and the multiple light guide lenses include multiple first filters that are disposed in multiple first insertion slots and multiple second filters that are disposed in multiple second insertion slots.

[0018] Each of the first filters is set at a 45° angle to the central axis of the corresponding first insertion slot, and each of the second filters is perpendicular to the central axis of the corresponding second insertion slot.

[0019] Optionally, the flow cytometer signal collection module further includes a limiting structure, which is disposed on the first base and at least partially extends into a plurality of the first insertion slots to press against one side of the light guide bracket.

[0020] Optionally, the limiting structure includes a first limiting pin locked to the first base body;

[0021] The plurality of light guide brackets include a plurality of first brackets that are inserted into a plurality of first insertion slots in a one-to-one correspondence. Each first bracket has a first surface for placing the first filter and a second surface that is parallel to the first surface and facing away from the first filter. The first surface is set at a 45° angle with the central axis of the corresponding light guide slot. The first limiting pin abuts against the second surface to restrict the first bracket within the first insertion slot. The first bracket is provided with a through hole extending longitudinally along the housing, and the through hole at least partially covers the first surface.

[0022] Optionally, each of the light guide brackets further includes a plurality of second brackets that are inserted into a plurality of second insertion slots in a one-to-one correspondence. Each second bracket has a third surface for placing the second filter. The third surface faces the first surface and is perpendicular to the central axis of the corresponding light guide slot.

[0023] The limiting structure includes a second limiting pin locked to the second base, the second limiting pin abutting against the side of the second bracket facing away from the partition plate.

[0024] Optionally, the openings of each of the plug slots are all facing upwards, and each of the first brackets and each of the second brackets has a first step portion and a second step portion. Each of the first step portions is located above the corresponding second step portions, and each of the second step portions abuts against the opening of the corresponding plug slot.

[0025] This utility model also provides a collection device, including the above-mentioned multiple flow cytometer signal collection modules, wherein the multiple flow cytometer signal collection modules are arranged adjacent to each other in the longitudinal direction of their housing.

[0026] The technical solution provided by this utility model has the following beneficial effects:

[0027] The flow cytometer signal collection module provided by this utility model includes a housing, an optical mounting base, a light guide component, an optical fiber assembly, and a detection component. The optical fiber assembly is used to calibrate the excitation fluorescence and conduct it towards the light guide lens located at the foremost end along the transverse direction of the housing. The optical mounting base has multiple insertion slots, each of which can accommodate a light guide support, thereby fixing the corresponding light guide lens at a preset position in the light guide slot. This allows the excitation fluorescence to be conducted according to the arrangement of the multiple light guide lenses, thus better transmitting light of the corresponding wavelength to the target location. At the aforementioned detection component, the acquired excitation fluorescence is analyzed to obtain the detection result. Moreover, multiple sets of insertion slots are provided, and all sets of insertion slots are integrated and arranged on the optical mounting base, thereby correspondingly arranging multiple sets of light guide lenses. Each set of light guide lenses can filter light of different wavelengths, making the structure of the entire flow cytometer signal collection module more compact and smaller in size. On the other hand, the flow cytometer signal collection module can be used to collect excitation fluorescence of multiple different wavelengths for simultaneous detection, resulting in higher detection efficiency and more reliable detection results. Attached Figure Description

[0028] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0029] Figure 1 A schematic diagram of the structure of an embodiment of a flow cytometer signal collection module provided by this utility model;

[0030] Figure 2 for Figure 1 A cross-sectional structural diagram of the flow cytometer signal collection module described herein;

[0031] Figure 3 for Figure 1 A schematic diagram of the optical mounting base and light guide component described herein;

[0032] Figure 4 for Figure 3 An exploded structural diagram of the optical mounting base and light guide component described herein;

[0033] Figure 5 for Figure 3 Another exploded view of the optical mounting base and light guide component described herein;

[0034] Figure 6 for Figure 5 An exploded view of the optical mounting base and light guide components described herein.

[0035] Explanation of icon numbers:

[0036] 100 - Flow cytometer signal collection module; 1 - Housing; 2 - Optical mounting base; 21 - First base; 22 - Second base; 23 - Baffle; 24 - First insertion slot; 25 - Second insertion slot; 3 - Light guide component; 31 - First support; 311 - First surface; 312 - Second surface; 32 - Second support; 33 - First filter; 34 - Second filter; 4 - Fiber optic assembly; 5 - Detection component; 6 - Limiting structure; 61 - First limiting pin; 62 - Second limiting pin.

[0037] The realization of the purpose, functional characteristics and excellent effects of this utility model will be further explained below in conjunction with specific embodiments and accompanying drawings. Detailed Implementation

[0038] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0039] It should be noted that if the embodiments of this utility model involve directional indication, the directional indication is only used to explain the relative positional relationship and movement of each component in a specific posture. If the specific posture changes, the directional indication will also change accordingly.

[0040] Furthermore, if the embodiments of this utility model involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the meaning of "and / or" throughout the text includes three parallel solutions; for example, "A and / or B" includes solution A, solution B, or a solution where both A and B are satisfied simultaneously. Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by this utility model.

[0041] This utility model provides a flow cytometer signal acquisition module 100. For details, please refer to... Figures 1 to 2 In this embodiment, the flow cytometer signal collection module 100 includes a housing 1, an optical mounting base 2, a light guide component 3, an optical fiber assembly 4, and a detection component 5. The optical mounting base 2 is disposed inside the housing 1 and has multiple insertion slots spaced apart along the transverse direction of the housing 1, and light guide slots communicating with each of the insertion slots. The light guide slots have an opening that penetrates the rear side of the optical mounting base 2. The light guide component 3 includes multiple light guide brackets and multiple light guide lenses corresponding to each of the multiple light guide brackets. The multiple light guide brackets are inserted into the insertion slots, such that the multiple light guide lenses are spaced apart along the transverse direction of the housing 1. The light guide groove is located within the housing 1; the optical fiber assembly 4 is disposed within the housing 1 and extends at least partially out of the housing 1. The optical fiber assembly 4 is used to guide the excited fluorescence toward the light guide lens located at the foremost end along the transverse direction of the housing 1, so that the light is reflected by the light guide lens and then guided along the direction of the light guide groove to exit from the opening; the detection assembly 5 is disposed within the housing 1 and is arranged corresponding to the opening; wherein, a plurality of insertion slots are arranged at intervals along the transverse direction of the housing 1 to form a set of insertion slots, and there are multiple sets of insertion slots, and the multiple sets of insertion slots are arranged sequentially along the longitudinal direction of the housing 1; there are multiple sets of light guide components 3, and each set of light guide components 3 is arranged corresponding to the set of insertion slots.

[0042] In this embodiment, the optical fiber assembly 4 is used to calibrate the excitation fluorescence and conduct it towards the light guide lens located at the foremost end along the transverse direction of the housing 1. The optical mounting base 2 has multiple insertion slots, each of which can accommodate a light guide bracket, thereby fixing the corresponding light guide lens at a preset position in the light guide slot. This allows the excitation fluorescence to be conducted according to the arrangement of the multiple light guide lenses, better guiding light of the corresponding wavelength to the detection component 5. The detection component 5 analyzes the acquired excitation fluorescence to obtain the detection result. Furthermore, multiple sets of insertion slots are provided, and these sets are integrated on the optical mounting base 2, allowing for the corresponding arrangement of multiple sets of light guide lenses. Each set of light guide lenses can filter light of different wavelengths, making the entire flow cytometer signal collection module 100 more compact and smaller in size. On the other hand, the flow cytometer signal collection module 100 can be used to collect excitation fluorescence of multiple different wavelengths for simultaneous detection, resulting in higher detection efficiency and more reliable detection results.

[0043] The housing 1 is generally L-shaped, having a first storage segment extending laterally and a second storage segment extending vertically. The optical mounting base 2 is located within the first storage segment, and the detection component 5 is correspondingly located within the second storage segment. When the flow cytometer signal collection module 100 is operating normally, the first storage segment is positioned in front of the second storage segment; all descriptions of orientation in this invention refer to this. Here, "lateral" refers to the front-to-back direction of the housing 1, and "vertical" refers to the left-to-right direction of the housing 1. The top cover of the first storage segment of the housing 1 is slidable for easy opening and closing, thus facilitating the installation and replacement of the light guide lens.

[0044] Preferably, a set of insertion slots includes two insertion slots, and the two insertion slots in the same set are spaced apart in the front-to-back direction, combined with Figure 4 As shown, the front insertion slot is designated as the first insertion slot 24, and the rear insertion slot is designated as the second insertion slot 25. The dimensions of the multiple insertion slots within the same group are different; that is, the dimensions of the first insertion slot 24 and the second insertion slot 25 are different, allowing for the installation of light guide brackets of different sizes to accommodate the installation requirements of light guide lenses of different sizes.

[0045] Multiple sets of insertion slots are arranged along the longitudinal direction (i.e., the left-right direction) of the housing 1, and the multiple first insertion slots 24 in the multiple sets of insertion slots are arranged opposite each other in the left-right direction, and the multiple second insertion slots 25 in the multiple sets of insertion slots are also arranged opposite each other in the left-right direction, and the multiple first insertion slots 24 are of the same size, and the multiple second insertion slots 25 are of the same size.

[0046] It is understood that, in one embodiment, the plurality of first insertion slots 24 or the plurality of second insertion slots 25 may be arranged separately for the placement of the plurality of light guide brackets.

[0047] Preferably, the plurality of first insertion slots 24 are interconnected, and the plurality of second insertion slots 25 are interconnected, so that the arrangement distance between two adjacent first insertion slots 24 and two adjacent second insertion slots 25 can be closer, and the plurality of first insertion slots 24 and the plurality of second insertion slots 25 occupy less space in the left and right direction. Therefore, the volume of the entire optical mounting base 2 can be smaller, and the volume of the entire flow cytometer signal collection module 100 can also be smaller.

[0048] Specifically, two receiving cavities are formed on the optical mounting base 2, extending longitudinally along the housing 1. The two receiving cavities are spaced laterally along the housing 1, and multiple partition protrusions are provided on the sidewalls of each receiving cavity. The space between each pair of adjacent partition protrusions forms a insertion slot, so that multiple first insertion slots 24 are connected and multiple second insertion slots 25 are connected. This allows for a smaller spacing between adjacent light guide brackets and a more compact arrangement of multiple light guide brackets, thus occupying less space.

[0049] For the optical mounting base 2, preferably, combined with Figures 4 to 6 As shown, the optical mounting base 2 includes a first base 21, a second base 22, and a partition plate 23. The first base 21 and the second base 22 are detachably connected. The first base 21 has a first semi-groove, and the second base 22 has a second semi-groove opposite to the first semi-groove. Both the first base 21 and the second base 22 are approximately U-shaped, with the open sides of the first base 21 and the second base 22 facing each other. The partition plate 23 is disposed between the first base 21 and the second base 22. The two sides of the partition plate 23 respectively block the open sides of the first semi-groove and the second semi-groove to form the two accommodating cavities mentioned above. Each accommodating cavity has multiple protruding partition bosses on its sidewall. The space between each pair of adjacent partition bosses forms a insertion slot, thereby allowing multiple light guide brackets to be placed. The optical mounting base 2 is designed as a split structure, which facilitates the processing of the first base 21, the second base 22 and the partition plate 23, and also facilitates the maintenance of the first base 21, the second base 22 and the partition plate 23, resulting in lower maintenance costs.

[0050] Furthermore, a first insertion groove 24 is formed between the first seat 21 and the partition plate 23, and a second insertion groove 25 is formed between the second seat 22 and the partition plate 23; a plurality of light guide lenses are provided, and the plurality of light guide lenses include a plurality of first filters 33 corresponding to a plurality of first insertion grooves 24, and a plurality of second filters 34 corresponding to a plurality of second insertion grooves 25; wherein, each first filter 33 is set at a 45º angle with the central axis of the corresponding first insertion groove, and each second filter 34 is perpendicular to the central axis of the corresponding second insertion groove. The first filter 33 can reflect and transmit light. A portion of the light transmitted from the optical fiber assembly 4 can be reflected by the first filter 33 to the corresponding second filter 34, and then transmitted to the detection assembly 5 after being filtered by the second filter 34. Another portion of the light passes through the first filter 33 and is transmitted to the next first filter 33 to reflect and transmit light of different wavelengths. In this way, multiple first filters 33 and multiple second filters 34 can be used to obtain multiple excitation fluorescence of different wavelengths.

[0051] Furthermore, the flow cytometer signal collection module 100 also includes a limiting structure 6, which is disposed on the first base 21 and can at least partially extend into the plurality of first insertion slots 24 to press against one side of the light guide bracket, so as to better fix the light guide bracket.

[0052] Preferably, combined with Figure 3 and Figure 5As shown, the limiting structure 6 includes a first limiting pin 61 locked to the first base 21; the plurality of light guide brackets include a plurality of first brackets 31 correspondingly inserted into a plurality of first insertion slots 24. Each first bracket 31 has a first surface 311 for placing the first filter 33 and a second surface 312 parallel to the first surface 311 and facing away from the first filter 33. The first surface 311 is set at a 45° angle with the central axis of the corresponding light guide groove, so that when the first filter 33 is installed on the first surface 311, it can also be set at a 45° angle with the central axis of the light guide groove, thereby better reflecting the excited fluorescence into the light guide groove, and then filtering stray light through the second filter 34. Preferably, the first limiting pin 61 abuts against the second surface 312 to confine the first bracket 31 within the first insertion slot 24. The first bracket 31 has a through hole extending longitudinally along the housing 1, the through hole at least partially covering the first surface 311. Through the through hole, a portion of the excited fluorescence can pass through the first bracket 31 located upstream of the light propagation to be conducted to the next first bracket 31. The first filter 33 can be fixed to the first bracket 31 by adhesive, snap-fit, or magnetic attraction.

[0053] Furthermore, such as Figure 5 As shown, each of the light guide brackets further includes a plurality of second brackets 32 correspondingly inserted into a plurality of second insertion slots 25. Each second bracket 32 ​​has a third surface for placing the second filter 34. The third surface faces the first surface 311 and is perpendicular to the central axis of the corresponding light guide groove. The excitation fluorescence reflected into the light guide groove by the first filter 33 can pass through the second filter 34 more parallelly. The second filter 34 can be fixed to the second bracket 32 ​​by adhesive, snap-fit, or magnetic attraction. The limiting structure 6 also includes a second limiting pin 62 locked to the second base 22. The second limiting pin 62 abuts against the side of the second bracket 32 ​​facing away from the partition plate 23 to fix the second bracket 32 ​​in the second insertion slot 25.

[0054] Furthermore, the openings of each of the insertion slots are all upward-facing. Each of the first brackets 31 and each of the second brackets 32 has a first step and a second step. Each first step is located above the corresponding second step, and each second step abuts against the opening of the corresponding insertion slot. The second step restricts the vertical position of the corresponding light guide bracket, and the sidewalls of each insertion slot restrict the circumferential position of the corresponding light guide bracket. This allows each light guide bracket to be stably accommodated within its corresponding insertion slot, better ensuring the position of the corresponding light guide lens. Moreover, the upward-facing openings of each insertion slot allow for easy removal of the light guide bracket, facilitating replacement of the light guide bracket and light guide lens. This allows the flow cytometer signal collection module 100 to better adapt to the installation requirements of different light guide lenses and to better meet the detection requirements of excitation fluorescence at different wavelengths.

[0055] The optical fiber assembly 4 includes an optical fiber bundle, a collimating mirror, and a reflecting mirror. The optical fiber bundle is used to transmit the excited fluorescence to the collimating mirror. After the light is calibrated, it is reflected by the reflecting mirror to the light guide lens, thereby acquiring multi-band fluorescence.

[0056] The detection component 5 includes a circuit board and multiple photodiodes electrically connected to the circuit board. The circuit board can be electrically connected to an external display device. The multiple photodiodes can acquire the excitation fluorescence emitted by multiple sets of light guide components 3 and convert the light signal into an electrical signal, thereby transmitting it to the external display device.

[0057] This invention also provides a modular flow cytometer signal collection device, which includes multiple flow cytometer signal collection modules 100. These modules are arranged sequentially and adjacently along the longitudinal direction of the housing 1. No circuit boards or other heat-generating devices are located at either end of each module 100, allowing for closer proximity between adjacent modules and preventing excessive heat generation. This results in a smaller overall size for the modular flow cytometer signal collection device. Furthermore, it allows for the simultaneous irradiation of sample cells with laser beams of different wavelengths after shaping and combining. The scattered light and excitation fluorescence generated after irradiation can be transmitted one-to-one to the multiple flow cytometer signal collection modules 100 via the multiple fiber optic components 4, effectively avoiding interference between different types of scattered light and fluorescence signals.

[0058] The above description is only a preferred embodiment of the present utility model and does not limit the patent scope of the present utility model. Any equivalent structure made using the contents of the present utility model specification and drawings, or directly or indirectly applied to other related technical fields, are similarly included within the patent protection scope of the present utility model.

Claims

1. A flow cytometer signal acquisition module, characterized in that, include: case; An optical mounting base is disposed within the housing. The optical mounting base has a plurality of insertion slots arranged at intervals along the transverse direction of the housing, and a light guide groove that communicates with all of the plurality of insertion slots. The light guide groove has an opening that penetrates the rear side of the optical mounting base. The light guide component includes multiple light guide brackets and multiple light guide lenses that are correspondingly disposed on the multiple light guide brackets. The multiple light guide brackets are inserted into the insertion slots, and the multiple light guide lenses are arranged in the light guide slots at lateral intervals along the housing. An optical fiber assembly is disposed within the housing and extends at least partially out of the housing. The optical fiber assembly is used to conduct the excited fluorescence toward the light guide lens located at the foremost end along the transverse direction of the housing, so that the light is reflected by the light guide lens and then conducted along the direction of the light guide groove to be emitted from the opening. The detection component is disposed inside the housing and is positioned corresponding to the opening; Multiple insertion slots are arranged at intervals along the transverse direction of the housing to form a set of insertion slots. Multiple sets of insertion slots are arranged sequentially along the longitudinal direction of the housing. Multiple sets of light guide components are provided, and each set of light guide components corresponds to a set of insertion slots.

2. The flow cytometer signal acquisition module as described in claim 1, characterized in that, The dimensions of the multiple insertion slots within the same group are set differently.

3. The flow cytometer signal acquisition module as described in claim 1, characterized in that, The optical mounting base has two accommodating cavities extending longitudinally along the housing. The two accommodating cavities are spaced laterally along the housing, and each accommodating cavity has a plurality of partition protrusions protruding from its sidewall. The space between each pair of adjacent partition protrusions forms a insertion groove.

4. The flow cytometer signal acquisition module as described in claim 1, characterized in that, The optical mounting base includes: The first seat and the second seat are detachably connected. The first seat has a first half-groove, and the second seat has a second half-groove that is opposite to the first half-groove. A partition plate is disposed between the first base and the second base. The two sides of the partition plate respectively block the open sides of the first half-groove and the second half-groove to form two receiving cavities. Multiple partition protrusions are provided on the side wall of each receiving cavity. The space between each pair of adjacent partition protrusions forms a insertion groove.

5. The flow cytometer signal acquisition module as described in claim 4, characterized in that, A first insertion groove is formed between the first base and the partition plate, and a second insertion groove is formed between the second base and the partition plate; The light guide lens is provided in multiple ways, and the multiple light guide lenses include multiple first filters that are disposed in multiple first insertion slots and multiple second filters that are disposed in multiple second insertion slots. Each of the first filters is set at a 45° angle to the central axis of the corresponding first insertion slot, and each of the second filters is perpendicular to the central axis of the corresponding second insertion slot.

6. The flow cytometer signal acquisition module as described in claim 5, characterized in that, The flow cytometer signal collection module further includes a limiting structure, which is disposed on the first base and at least partially extends into a plurality of the first insertion slots to press against one side of the light guide bracket.

7. The flow cytometer signal acquisition module as described in claim 6, characterized in that, The limiting structure includes a first limiting pin that is locked onto the first base body; The plurality of light guide brackets include a plurality of first brackets that are inserted into a plurality of first insertion slots in a one-to-one correspondence. Each first bracket has a first surface for placing the first filter and a second surface that is parallel to the first surface and facing away from the first filter. The first surface is set at a 45° angle with the central axis of the corresponding light guide slot. The first limiting pin abuts against the second surface to restrict the first bracket within the first insertion slot. The first bracket is provided with a through hole extending longitudinally along the housing, and the through hole at least partially covers the first surface.

8. The flow cytometer signal acquisition module as described in claim 7, characterized in that, Each of the light guide brackets further includes a plurality of second brackets that are inserted into a plurality of second insertion slots in a one-to-one correspondence. Each second bracket has a third surface for placing the second filter. The third surface faces the first surface and is perpendicular to the central axis of the corresponding light guide slot. The limiting structure includes a second limiting pin locked to the second base, the second limiting pin abutting against the side of the second bracket facing away from the partition plate.

9. The flow cytometer signal acquisition module as described in claim 8, characterized in that, Each of the plug slots has its opening facing upwards. Each of the first brackets and each of the second brackets has a first step and a second step. Each of the first steps is located above the corresponding second step, and each of the second steps abuts against the opening of the corresponding plug slot.

10. A collection device, characterized in that, It includes a plurality of flow cytometer signal collection modules as described in any one of claims 1 to 9, wherein the plurality of flow cytometer signal collection modules are arranged sequentially adjacent to each other along the longitudinal direction of its housing.

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