Automatic magnetic bead purifier

Abstract

This utility model belongs to the field of sample pretreatment technology, specifically an automatic magnetic bead purifier, including a housing. Inside the housing is a movable frame with several magnetic rods fixedly mounted on it. Below the magnetic rods is a loading tray with multiple purification well groups. Each purification well group has its pores filled with a corresponding liquid. Each magnetic rod corresponds to one purification well group. The magnetic rods sequentially enter the pores of each purification well group, purifying the sample during this process. This utility model effectively improves the efficiency of sample purification. This magnetic bead purifier uses magnetic force to separate magnetic beads from adsorbed molecules, thereby achieving the purpose of separation and purification. It achieves reliable high recovery rates and consistent performance in a series of complex samples.

Description

Technical Field

[0001] This utility model relates to the field of sample pretreatment technology, specifically to an automatic magnetic bead purifier. Background Technology

[0002] Magnetic bead purifiers are devices used for sample pretreatment, concentration, and purification before analytical experiments to extract and purify toxins from samples. Currently, the enrichment and purification of fungal toxins in samples in laboratories mainly uses immunoaffinity columns. This method uses a syringe as the loading container for immunoproteins. To prevent leakage, sieve plates are installed above and below the immunoprotein to allow liquids and soluble samples to pass through the column, while the immunoproteins remain inside. This results in high pressure when passing through the column for some complex samples, affecting the adsorption of the detected components and making manual operation difficult. Although fully automated solid-phase extraction devices and immunoaffinity columns are being designed to automate the immunoaffinity process, the high pressure and inconsistent sample recovery rates associated with complex samples remain unresolved. This invention provides an automated magnetic bead purifier that uses magnetic force to separate magnetic beads from adsorbed molecules, achieving separation and purification. Effective magnetic bead collection and transfer facilitate better washing, efficient elution, and rapid processing, resulting in reliable high recovery rates and consistent performance across a range of complex samples. Utility Model Content

[0003] In order to overcome the above-mentioned defects in the existing technology, this utility model provides an automatic magnetic bead purifier.

[0004] An automatic magnetic bead purifier includes a housing with a movable frame inside. Several magnetic rods are fixedly mounted on the movable frame. A loading tray is located below the magnetic rods. The loading tray has multiple purification well groups. Each purification well group has a corresponding liquid filling its cavity. Each magnetic rod corresponds to one purification well group. The magnetic rods sequentially enter the cavities of each purification well group to purify the sample.

[0005] Furthermore, each of the purification well groups includes a first well containing sample diluent, a second well containing immunomagnetic beads, a third and a fourth well containing diluent and washing solution, and a fifth well containing elution solution.

[0006] Furthermore, the volume of the fifth hole is greater than the volume of the other holes.

[0007] Furthermore, the movable frame is slidably mounted on the column, the column is equipped with a lifting screw, the lifting screw is pre-moving the frame and connected, the top of the lifting screw is connected to the drive motor, and the drive motor is fixedly mounted on the column.

[0008] Furthermore, the bottom of the upright column is slidably connected to the horizontal column, and the horizontal column is provided with a horizontal lead screw, which is connected to a rotary motor, and the rotary motor is fixedly installed on the horizontal column.

[0009] Furthermore, the bottom inner wall of the housing is provided with a limiting groove for placing the loading tray.

[0010] Furthermore, a magnetic rod sleeve is fitted over the bottom of the magnetic rod.

[0011] Furthermore, the top of the magnetic rod sleeve is provided with a mounting plate, the mounting plate is provided with a locking block, the movable frame is provided with a buckle, and the locking block and the buckle are connected in cooperation.

[0012] Furthermore, a display screen is provided on the outer wall of the housing.

[0013] Due to the adoption of the above technical solutions, the beneficial technical effects of this utility model are as follows: In this utility model, the moving frame drives the magnetic rod to rise and fall, so that the magnetic rod can be inserted into the purification well from top to bottom. Each magnetic rod corresponds to a purification well group. In addition, the moving frame can move horizontally, so that each magnetic rod can enter each cavity of the purification well group in sequence. During this process, the magnetic rod separates the magnetic beads and adsorbed molecules, and purifies the sample through the liquid in the cavity, effectively improving the sample purification efficiency. The magnetic bead purifier designed here separates the magnetic beads and adsorbed molecules through magnetic force, thereby achieving the purpose of separation and purification, and achieving reliable high recovery rate and consistent performance in a series of complex samples. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the structure of an automatic magnetic bead purifier according to the present invention;

[0015] Figure 2 This is a front view of an automatic magnetic bead purifier according to the present invention;

[0016] Figure 3 This is a schematic diagram of the internal structure of the casing in this utility model;

[0017] Figure 4 This is a schematic diagram of the structure of the magnetic rod and the movable frame in this utility model;

[0018] Figure 5 This is a schematic diagram of the structure of the magnetic rod sleeve and the mounting plate in this utility model.

[0019] In the diagram: 1. Housing; 2. Moving frame; 3. Magnetic rod; 4. Loading tray; 5. First hole; 6. Second hole; 7. Third hole; 8. Fourth hole; 9. Fifth hole; 10. Column; 11. Lifting screw; 12. Drive motor; 13. Horizontal column; 14. Horizontal screw; 15. Rotary motor; 16. Limiting groove; 17. Magnetic rod sleeve; 18. Mounting plate; 19. Locking block; 20. Buckle; 21. Display screen. Detailed Implementation

[0020] To more clearly illustrate the technical solution of this utility model, the following description is made in conjunction with the accompanying drawings. Obviously, the drawings described below are only one embodiment of this utility model. For those skilled in the art, other embodiments can be obtained based on these drawings and embodiments without creative effort, and all of them fall within the protection scope of this utility model.

[0021] according to Figure 1-5 As shown, an automatic magnetic bead purifier includes a housing 1, inside which is a movable frame 2. Several magnetic rods 3 are fixedly installed on the movable frame 2. Below the magnetic rods 3 is a loading tray 4. The loading tray has multiple purification well groups. The cavity of each purification well group is filled with a corresponding liquid. Each magnetic rod 3 corresponds to one purification well group. The magnetic rods 3 enter the cavity of each purification well group in sequence to purify the sample during this process.

[0022] In the specific technical solution described above, the moving frame 2 drives the magnetic rods 3 to move up and down, allowing the magnetic rods 3 to be inserted into the purification wells from top to bottom. Each magnetic rod 3 corresponds to one purification well group. Furthermore, the moving frame 2 can move horizontally, allowing each magnetic rod 3 to sequentially enter each well of the purification well group. During this process, the magnetic rods 3 separate the magnetic beads and adsorbed molecules, and purify the sample through the liquid in the well, effectively improving the sample purification efficiency. Preferably, using more than two magnetic rods 3 can further improve the sample purification efficiency. This magnetic bead purifier design uses magnetic force to separate magnetic beads and adsorbed molecules, thereby achieving the purpose of separation and purification, achieving reliable high recovery rates and consistent performance in a range of complex samples.

[0023] Each purification well group includes a first well 5 containing sample diluent, a second well 6 containing immunomagnetic beads, a third well 7 and a fourth well 8 containing diluent and washing solution, and a fifth well 9 containing elution solution. The magnetic rod 3 is sequentially inserted into the first well 5, the second well 6, the third well 7, the fourth well 8 and the fifth well 9 to achieve sample purification.

[0024] The volume of the fifth well 9 is larger than that of the other wells, which can increase the content of the eluent in the fifth well 9 and improve the purification effect.

[0025] The movable frame 2 is slidably mounted on the column 10. The column 10 is provided with a lifting screw 11, which is connected to the movable frame 2. The top of the lifting screw 11 is connected to the drive motor 12, which is fixedly mounted on the column 10.

[0026] The bottom of the upright column 10 is slidably connected to the horizontal column 13. The horizontal column 13 is provided with a horizontal lead screw 14, which is connected to a rotary motor 15. The rotary motor 15 is fixedly installed on the horizontal column 13.

[0027] The aforementioned movable frame 2 can move up and down along the height of the column 10, thereby allowing the magnetic rod 3 to be inserted into the cavity and to move upward to disengage the magnetic rod 3 from the cavity. In addition, when the magnetic rod 3 is inserted into the cavity, the drive motor 12 can be controlled to drive the magnetic rod 3 to move up and down at high speed along the Z-axis to achieve full mixing of the magnetic bead and the sample. Through the horizontal movement of the column 10, the magnetic rod 3 sequentially enters the first hole 5, the second hole 6, the third hole 7, the fourth hole 8, and the fifth hole 9.

[0028] The bottom inner wall of the housing 1 is provided with a limiting groove 16 for placing the loading tray 4, which facilitates the positioning of the loading tray 4 and the replacement of the loading tray 4.

[0029] The bottom of the magnetic rod 3 is fitted with a magnetic rod sleeve 17, which can directly contact the sample. After each set of samples is completed, the magnetic rod sleeve 17 needs to be recycled and cleaned before it can be reused. The magnetic rod 3 does not come into contact with the sample and does not need to be cleaned, which improves the efficiency of sample purification.

[0030] The top of the magnetic rod sleeve 17 is provided with a mounting plate 18, and the mounting plate 18 is provided with a locking block 19. The movable frame 2 is provided with a buckle 20. The locking block 19 and the buckle 20 are connected in cooperation. Through the cooperation of the locking block 19 and the buckle 20, the mounting plate 18 is connected to the movable frame 2, so that the magnetic rod sleeve 17 is placed on the magnetic rod 3. After the sample purification is completed, the connection between the locking block 19 and the buckle 20 is released, so as to facilitate the replacement of the magnetic rod sleeve 17. It is worth noting that the locking block 19 and the buckle 20 are existing technologies, and their specific structures will not be described in detail here.

[0031] The outer wall of the housing 1 is provided with a display screen 21. The operation panel of the display screen 21 uses a 10-inch touch screen as the human-computer interaction interface. On the screen, operations such as scheme setting and data storage can be performed to achieve convenient sample processing.

[0032] The control module is located inside the casing 1 and is hidden behind the operation panel. It consists of a power module, a main control board and a drive module.

[0033] The above embodiments are merely exemplary embodiments of the present utility model and are not intended to limit the present utility model. The scope of protection of the present utility model is defined by the claims. Those skilled in the art can make various modifications or equivalent substitutions to the present utility model within its substance and scope of protection, and such modifications or equivalent substitutions should also be considered to fall within the scope of protection of the present utility model.

Claims

1. An automatic magnetic bead purifier, comprising a housing (1), characterized in that, The housing (1) is provided with a movable frame (2) inside. Several magnetic rods (3) are fixedly installed on the movable frame (2). A loading tray (4) is provided below the magnetic rods (3). Multiple purification well groups are provided on the loading tray (4). Each purification well group is filled with a corresponding liquid. Each magnetic rod (3) corresponds to a purification well group. The magnetic rods (3) enter the wells of each purification well group in sequence to purify the sample during this process.

2. The automatic magnetic bead purifier according to claim 1, characterized in that, Each of the purification well groups includes a first well (5) containing sample diluent, a second well (6) containing immunomagnetic beads, a third well (7) and a fourth well (8) containing diluent and washing solution, and a fifth well (9) containing elution solution.

3. An automatic magnetic bead purifier according to claim 2, characterized in that, The volume of the fifth hole (9) is greater than the volume of the other holes.

4. An automatic magnetic bead purifier according to claim 1, characterized in that, The movable frame (2) is slidably mounted on the column (10). The column (10) is provided with a lifting screw (11). The lifting screw (11) is connected to the movable frame (2). The top of the lifting screw (11) is connected to the drive motor (12). The drive motor (12) is fixedly mounted on the column (10).

5. An automatic magnetic bead purifier according to claim 4, characterized in that, The bottom of the column (10) is slidably connected to the horizontal column (13). The horizontal column (13) is provided with a horizontal lead screw (14). The horizontal lead screw (14) is connected to a rotary motor (15). The rotary motor (15) is fixedly installed on the horizontal column (13).

6. An automatic magnetic bead purifier according to claim 1, characterized in that, The bottom inner wall of the housing (1) is provided with a limiting groove (16) for placing the loading tray (4).

7. An automatic magnetic bead purifier according to claim 1, characterized in that, The bottom of the magnetic rod (3) is fitted with a magnetic rod sleeve (17).

8. An automatic magnetic bead purifier according to claim 7, characterized in that, The top of the magnetic rod sleeve (17) is provided with an installation plate (18), the installation plate (18) is provided with a locking block (19), the movable frame (2) is provided with a buckle (20), and the locking block (19) and the buckle (20) are connected in cooperation.

9. An automatic magnetic bead purifier according to claim 1, characterized in that, The outer wall of the housing (1) is provided with a display screen (21).

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