A low-temperature box special for rapid delivery of a cell sample

Abstract

The utility model discloses a kind of cell sample rapid special low-temperature box for delivery, belong to biological sample preservation device field, including box assembly, box assembly is double-layer structure, box assembly is successively nested by shell, vacuum heat insulation layer and inner container composition;Refrigeration module, refrigeration module is embedded in the bottom of inner container, refrigeration module includes adsorbent groove and liquid nitrogen injection port, adsorbent groove is filled with liquid nitrogen adsorption material, and liquid nitrogen injection port is set in the top of box assembly and is communicated with adsorbent groove;Sample fixing module, sample fixing module is attached to refrigeration module;Sealing cover assembly, sealing cover assembly is movably connected in the top of box assembly, to realize open and close sealing, the utility model is small in size, convenient to operate, can shorten single batch sample picking time, improve sample recovery survival rate, applicable to the delivery screening scene of various cell laboratories and biological sample library.

Description

Technical Field

[0001] This utility model belongs to the field of biological sample preservation devices, and more specifically, relates to a special low-temperature box for rapid release of cell samples. Background Technology

[0002] In cell biology experiments and clinical sample studies, cell samples typically need to be preserved long-term in a liquid nitrogen environment at -196°C to maintain their viability. When samples are taken out of storage, cryopreservation tubes need to be removed from the liquid nitrogen tank and target samples need to be screened. In this process, there is a core contradiction between the low-temperature preservation of samples and the efficiency of sample selection.

[0003] In existing technologies, sample retrieval is usually carried out in two ways: one is to directly search for samples one by one from the liquid nitrogen tank, which takes 10-15 minutes per batch. The samples are easily exposed to liquid nitrogen vapor or room temperature environment, which can cause the temperature to rise, resulting in decreased cell activity or even inactivation; the other is to use commercial large-scale cryogenic transport boxes. Although they can maintain low temperature, they are bulky (usually external dimensions ≥50cm×30cm×20cm) and weigh more than 5kg, which is inconvenient to operate in biosafety cabinets. Moreover, the manufacturing cost is as high as 5,000-8,000 yuan, and the liquid nitrogen consumption is more than 30mL per hour, resulting in high long-term use costs.

[0004] Chinese utility model patent CN209840355U discloses a cryogenic transport box for biological samples, which uses a compressor refrigeration method. Although it can maintain low temperatures, it requires an external power supply and cannot be used flexibly next to liquid nitrogen tanks or inside biosafety cabinets. Patent CN211502298U discloses a portable liquid nitrogen storage box, which focuses on long-distance transport but lacks an internal positioning structure, resulting in low sample picking efficiency. Existing technologies have not solved the problem of synergistically combining "miniaturized and convenient operation" with "rapid sample picking + stable low temperature," highlighting the urgent need for a dedicated cryogenic device suitable for sample bank outbound scenarios. Utility Model Content

[0005] The main purpose of this invention is to provide a low-temperature box specifically designed for rapid cell sample retrieval. It is compact, easy to operate, and suitable for screening scenarios in various cell laboratories and biobanks.

[0006] According to a first aspect of the present invention, a special low-temperature chamber for rapid release of cell samples is provided, comprising: The enclosure assembly has a double-layer structure, consisting of an outer shell, a vacuum insulation layer, and an inner liner nested together in sequence. A refrigeration module is embedded in the bottom of the inner liner. The refrigeration module includes an adsorbent groove and a liquid nitrogen injection port. The adsorbent groove is filled with liquid nitrogen adsorbent material, and the liquid nitrogen injection port is located at the top of the box assembly and communicates with the adsorbent groove. A sample fixation module is attached to and connected to the cooling module. A sealing cap assembly is movably connected to the top of the housing assembly to achieve opening and closing sealing.

[0007] According to the first aspect of the present invention, the cell sample rapid release low-temperature box is made of stainless steel with a thickness of 1.5-2mm; the inner liner is made of aluminum with a thickness of 1-1.2mm; and the vacuum insulation layer is made of vacuum glass wool and polyurethane composite with a thickness of 3-4mm.

[0008] According to the first aspect of the present invention, the cell sample rapid release low temperature box is provided, wherein the sample fixation module is configured as a detachable silicone fixation frame, wherein multiple independent slots are evenly distributed on the detachable silicone fixation frame, each of the independent slots is provided with a unique number, and cryopreservation tubes can be inserted into each of the independent slots, and the bottom of the detachable silicone fixation frame is provided with a positioning boss that matches the adsorbent groove.

[0009] According to the first aspect of the present invention, the cell sample rapid release low-temperature box has the dimensions of 2.2cm×2.2cm×3.8cm for the independent card slot, the dimensions of the cryopreservation tube are 1.8mL, and the center-to-center distance between adjacent independent card slots is 3cm.

[0010] According to the first aspect of the present invention, the cell sample rapid release low-temperature box includes a sliding cover plate, a sealing ring, and a transparent observation window. The sliding cover plate is slidably connected to the top of the box assembly via a slide rail. The sealing ring is installed on the inner edge of the sliding cover plate. The transparent observation window is made of low-temperature resistant glass and is fixedly installed in the middle of the sliding cover plate. The sliding cover plate has a liquid nitrogen pressure relief hole.

[0011] The cell sample rapid release low-temperature chamber according to the first aspect of the present invention further includes a safety monitoring module. The safety monitoring module includes a temperature sensor, a pressure sensor, an alarm light, and a safety valve. The temperature sensor and the pressure sensor are respectively installed on the side wall of the inner liner and the chamber assembly. The temperature sensor is connected to a temperature display, which is fixedly installed on the outer shell. The alarm light and the safety valve are installed on the top of the chamber assembly.

[0012] According to the first aspect of the present invention, the cell sample rapid release low-temperature box is provided with anti-frost silicone handles on the side walls of the box assembly, and anti-slip rubber pads on the bottom of the box assembly, and a sealing plug is connected to the liquid nitrogen injection port.

[0013] According to the first aspect of the present invention, the cell sample rapid release low-temperature box is provided with porous activated carbon as the liquid nitrogen adsorption material, and the adsorbent groove is provided with a detachable cover plate, and the detachable cover plate and the adsorbent groove are sealed together by a sealing gasket.

[0014] According to the first aspect of the present invention, the cell sample rapid release low-temperature chamber includes a refrigeration module that further includes a liquid nitrogen discharge port, which is disposed on the side wall of the chamber assembly and communicates with the adsorbent groove.

[0015] According to the first aspect of the present invention, the cell sample rapid release low-temperature box has an external dimension of 28cm×18cm×8cm and an effective volume of 25cm×15cm×4cm.

[0016] One of the above-described technical solutions of this utility model has at least one of the following advantages or beneficial effects: This invention reduces the thermal conductivity of the chamber by using a box assembly. Liquid nitrogen is added through a liquid nitrogen injection port and liquid nitrogen adsorption refrigeration is adopted. The liquid nitrogen adsorption material slowly releases cold energy after adsorbing liquid nitrogen to maintain the low temperature, thus keeping the sample at a low temperature. The sealing cap assembly can be opened and closed with one hand. This low temperature chamber is compact and easy to operate, which can shorten the single batch sample picking time and improve the sample recovery survival rate. It is suitable for various cell laboratories and biobank sample screening scenarios. Attached Figure Description

[0017] The present invention will be further described below with reference to the accompanying drawings and embodiments; Figure 1 This is a schematic diagram of the structure of the low-temperature box for rapid cell sample release in the first embodiment of this utility model; Figure 2 This is a schematic diagram of the sample fixing module in the first embodiment of this utility model. Detailed Implementation

[0018] The embodiments of this utility model are described in detail below. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this utility model, and should not be construed as limiting this utility model.

[0019] In the description of this utility model, it should be understood that the directional descriptions, such as up, down, front, back, left, right, etc., indicate the directional or positional relationship based on the directional or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0020] In the description of this utility model, "several" means one or more, "multiple" means two or more, "greater than," "less than," and "exceeding" are understood to exclude the stated number, while "above," "below," and "within" are understood to include the stated number. If "first" or "second" is used in the description, it is only for the purpose of distinguishing technical features and should not be construed as indicating or implying relative importance, or implicitly indicating the number of indicated technical features, or implicitly indicating the order of the indicated technical features.

[0021] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" and "second" may explicitly or implicitly include one or more features.

[0022] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the term "connection" should be interpreted broadly. For example, it can be a fixed connection or a movable connection, a detachable connection or a non-detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection or a connection that can communicate with each other; it can be a direct connection or an indirect connection through an intermediate medium; it can be a connection within two elements, an indirect connection, or an interaction between two elements.

[0023] The following disclosure provides many different implementation methods or examples for different solutions to implement this utility model.

[0024] Reference Figures 1 to 2 As shown, a low-temperature chamber specifically designed for rapid unloading of cell samples is provided, comprising: The enclosure assembly has a double-layer structure, consisting of an outer shell, a vacuum insulation layer, and an inner liner nested together in sequence. The refrigeration module is embedded in the bottom of the inner liner. The refrigeration module includes an adsorbent groove and a liquid nitrogen injection port. The adsorbent groove is filled with liquid nitrogen adsorbent material, and the liquid nitrogen injection port is located at the top of the box assembly and communicates with the adsorbent groove. Sample fixing module 2 is attached and connected to the cooling module. A sealing cover assembly is movably connected to the top of the housing assembly to achieve opening and closing sealing.

[0025] In some embodiments of this utility model, the outer shell 1 is made of stainless steel and has a thickness of 1.5-2mm; the inner liner is made of aluminum and has a thickness of 1-1.2mm; the vacuum insulation layer is made of vacuum glass wool and polyurethane composite and has a thickness of 3-4mm.

[0026] In some embodiments of this utility model, the sample fixing module 2 is configured as a detachable silicone fixing frame. The detachable silicone fixing frame has multiple independent slots 21 evenly distributed on it. Each independent slot 21 is assigned a unique number. Each independent slot 21 can be fitted with a cryopreservation tube. The bottom of the detachable silicone fixing frame is provided with a positioning boss 22 that matches the adsorbent groove.

[0027] Furthermore, the dimensions of the independent card slot 21 are 2.2cm×2.2cm×3.8cm, the dimensions of the cryopreservation tube are 1.8mL, and the center-to-center distance between adjacent independent card slots 21 is 3cm.

[0028] In some embodiments of this utility model, the sealing cover assembly includes a sliding cover plate 31, a sealing ring 32, and a transparent observation window 33. The sliding cover plate 31 is slidably connected to the top of the housing assembly via a slide rail. The sealing ring 32 is installed on the inner edge of the sliding cover plate 31. The transparent observation window 33 is made of low-temperature resistant glass and is fixedly set in the middle of the sliding cover plate 31. A liquid nitrogen pressure relief hole is provided on the sliding cover plate 31. The sliding cover plate 31 is slidably connected to the housing assembly via a slide rail, and the single opening time is ≤2 seconds. The sealing ring 32 is embedded in the inner edge of the sliding cover plate 31, and the sealing performance after closing meets the requirement that the temperature rise within 30 seconds in an environment of -160℃ is ≤5℃. The transparent observation window 33 is made of low-temperature resistant glass, embedded in the middle of the sliding cover plate 31, and its area is not less than 1 / 3 of the total area of ​​the sliding cover plate 31.

[0029] In some embodiments of this invention, a safety monitoring module is also included. This module comprises a temperature sensor, a pressure sensor, an alarm light, and a safety valve. The temperature sensor and pressure sensor are respectively installed on the side walls of the inner liner and the housing assembly. The temperature sensor is connected to a temperature display, which is fixedly mounted on the outer casing 1. The alarm light and safety valve are installed on the top of the housing assembly. The alarm light activates when the inner liner temperature is ≥-150℃ or the internal pressure is ≥0.1MPa; the safety valve opening pressure threshold is set to 0.12MPa.

[0030] In some embodiments of this invention, the side wall of the housing assembly is provided with an anti-frost silicone handle 4, the bottom of the housing assembly is provided with an anti-slip rubber pad 5, and the liquid nitrogen injection port is connected with a sealing plug. The inner diameter of the liquid nitrogen injection port is 8-10mm, suitable for a 100mL portable liquid nitrogen filling container.

[0031] In some embodiments of this utility model, the liquid nitrogen adsorption material is porous activated carbon, the adsorbent groove is provided with a detachable cover plate, and the detachable cover plate and the adsorbent groove are sealed together by a sealing gasket. The amount of porous activated carbon is 40-60g.

[0032] In some embodiments of this utility model, the refrigeration module further includes a liquid nitrogen discharge port 6, which is disposed on the side wall of the housing assembly and communicates with the adsorbent groove.

[0033] In some embodiments of this utility model, the external dimensions of the box assembly are 28cm×18cm×8cm, and the effective volume of the inner liner is 25cm×15cm×4cm.

[0034] This invention comprises an outer casing, an insulated inner liner, a liquid nitrogen adsorption refrigeration module, a detachable silicone holder, and a sealing cap. The outer casing is made of 304 stainless steel, with an internal vacuum insulation layer and an aluminum inner liner. The liquid nitrogen adsorption refrigeration module contains porous activated carbon adsorbent, working in conjunction with a precise liquid nitrogen injection port to achieve a continuous low-temperature environment of -160℃±5℃. The detachable silicone holder has numbered independent slots to accommodate 1.8mL standard cryopreservation tubes and enable rapid positioning. The sealing cap uses a side-sliding structure, with a single opening time of ≤2 seconds and a temperature rise of ≤5℃. This invention is compact (external dimensions of the casing components are 28cm×18cm×8cm), easy to operate, and can reduce the sample picking time for a single batch from 10-15 minutes to 2-3 minutes, increasing the sample recovery survival rate by 12%-15%. It is suitable for various cell laboratories and biobanks for sample screening.

[0035] Cabinet Components: The cabinet employs a double-layer nested structure consisting of an outer shell, a vacuum insulation layer, and an inner liner. The outer shell is made of 304 stainless steel for enhanced corrosion resistance, while the inner liner uses high thermal conductivity aluminum to ensure temperature uniformity. The intermediate vacuum insulation layer is composed of vacuum glass wool and polyurethane composite material to reduce the thermal conductivity coefficient. The external dimensions are designed to be 28cm × 18cm × 8cm, fitting comfortably within the operating space of a biosafety cabinet. Weighing only 1.2kg (unloaded), it can be easily moved by a single person.

[0036] Refrigeration module: Utilizes liquid nitrogen adsorption refrigeration. The bottom of the inner liner has an adsorbent groove filled with 40-60g of porous activated carbon adsorbent. After adsorbing liquid nitrogen, it slowly releases cooling energy to maintain the low temperature. The side wall of the chamber has a 10mm inner diameter liquid nitrogen inlet equipped with a sealing plug, allowing for "on-demand cooling" via a portable refill container. Continuous operation can reach 4-6 hours, with liquid nitrogen consumption reduced to ≤10mL per hour.

[0037] Sample fixation module: Designed as a detachable silicone holder with multiple independent slots arranged in an array. Each slot is uniquely numbered and corresponds to the sample library registration system for rapid positioning. The individual slots are sized to fit 1.8mL standard cryovials, and the 3cm spacing between adjacent slots ensures that tweezers do not collide with adjacent samples during handling. The positioning protrusions on the bottom of the detachable silicone holder precisely match the adsorbent grooves, ensuring uniform temperature (±3℃) within the individual slots.

[0038] Sealing cap assembly: Adopts a side-sliding structure, allowing for one-handed opening and closing via a sliding rail, with an opening time ≤2 seconds; a sealing ring 32 is embedded inside the side-sliding cap 31, forming an effective seal after closure, with a temperature rise ≤5℃ within 30 seconds. A low-temperature resistant transparent glass observation window 33 is embedded in the center of the side-sliding cap 31, allowing direct observation of the internal sample number and reducing unnecessary cap openings.

[0039] Safety monitoring module: Built-in temperature sensor monitors the inner tank temperature in real time, pressure sensor monitors the internal evaporation pressure, and top alarm light activates to indicate when the temperature is ≥-150℃ or the pressure is ≥0.1MPa; equipped with a safety valve with an opening pressure of 0.12MPa to prevent danger caused by excessive pressure.

[0040] The beneficial effects of this utility model are: Significantly improved efficiency: The numbered independent card slot design reduces the single-batch sampling time from 10-15 minutes to 2-3 minutes, making it suitable for high-throughput screening scenarios. Laboratory tests show that the screening efficiency for 1000+ samples has increased by 400%. Sample viability protection: The system continuously maintains a low temperature environment of -160℃±5℃, and the exposure temperature of the sample throughout the process is ≤-155℃. The recovery survival rate is 12%-15% higher than that of traditional methods, solving the problem of loss of viability of precious samples. High ease of operation: The miniaturized and lightweight design is compatible with operation next to biosafety cabinets and liquid nitrogen tanks. It can be opened and closed and sample picking can be completed with one hand without additional manpower; the detachable silicone holder is easy to clean and disinfect, and is suitable for laboratory aseptic requirements. Significant cost advantages: manufacturing costs are only 1 / 3 to 1 / 5 of those of commercial transport boxes; liquid nitrogen consumption is only 1 / 3 of that of traditional equipment, and long-term use can significantly reduce operation and maintenance costs.

[0041] Example 1: Structural composition of a low-temperature chamber for rapid cell sample release The cell sample rapid release low-temperature chamber of this embodiment includes a chamber assembly, a refrigeration module, a sample fixation module, a sealing cap assembly, and a safety monitoring module. The specific structural parameters are as follows: Cabinet components: The outer shell is made of 304 stainless steel with a thickness of 1.8mm; the inner liner is made of pure aluminum with a thickness of 1.1mm; the vacuum insulation layer is 3.5mm thick and is composed of vacuum glass wool (2mm thick) and polyurethane (1.5mm thick); the external dimensions are 28cm×18cm×8cm, and the effective volume of the inner liner is 25cm×15cm×4cm; the side walls are equipped with anti-frost silicone handles that are 10cm long and 3cm wide, and the bottom is equipped with 4 anti-slip rubber pads with a diameter of 2cm.

[0042] Refrigeration module: The adsorbent groove is 20cm×10cm×1cm in size and located in the center of the bottom of the inner liner; it is filled with 50g of porous activated carbon adsorbent with a specific surface area ≥1500m² / g; the right side wall of the chamber is equipped with a liquid nitrogen injection port with an inner diameter of 10mm and a sealing plug, which is compatible with a 100mL graduated portable liquid nitrogen filling container.

[0043] Sample fixing module: The detachable silicone fixing frame measures 24cm×14cm×3.8cm and has multiple independent slots with an inner diameter of 2.2cm×2.2cm×3.8cm, each marked with a unique number; the bottom of the detachable silicone fixing frame has a 20cm×10cm×0.5cm positioning boss that matches the adsorbent groove, and the surface of the positioning boss is covered with a thermally conductive silicone layer.

[0044] Sealing cover assembly: The side-sliding cover measures 28cm×18cm×1cm and is made of ABS engineering plastic; a 3mm diameter silicone sealing ring with a circular cross-section is embedded on the inside; a 15cm×8cm low-temperature resistant borosilicate glass observation window with a light transmittance ≥90% is embedded in the middle of the side-sliding cover; the side-sliding cover is connected to the top of the box assembly by two parallel sliding rails with a sliding resistance ≤5N.

[0045] Safety monitoring module: The temperature sensor is a PT100 type, with a measurement range of -200℃ to 50℃ and an accuracy of ±0.5℃. The probe is embedded in the inner side wall of the liner. The pressure sensor has a measurement range of 0-0.2MPa and an accuracy of ±0.01MPa. The alarm light is a red LED light with a power of 0.5W, installed at the top front end of the enclosure assembly. The safety valve is model A28H-16, with an opening pressure of 0.12MPa, and is installed at the top rear end of the enclosure assembly.

[0046] Example 2: How to use a low-temperature chamber for rapid cell sample release The cell sample unloading operation was performed using the low-temperature chamber described in Example 1, and the steps are as follows: Pre-cooling preparation: 30 minutes before leaving the warehouse, remove the sealing plug of the liquid nitrogen injection port, inject 50mL of liquid nitrogen into the adsorbent groove through the portable filling bottle, close the sealing plug and put on the side sliding cover, and let it stand for 30 minutes; confirm that the inner tank temperature has stabilized to -160℃±5℃ by displaying the temperature sensor.

[0047] Sample transfer: Take the samples to be screened from the liquid nitrogen tank according to the release list, and place them into the independent slots of the detachable silicone holder according to their numbers. The sample information is registered in the format of "numerical number-sample type-preparation date" (e.g., "3-lung cancer organoid sample-LC-20240512"). The single exposure time during the transfer process is ≤10 seconds.

[0048] Sampling procedure: Place the cryogenic chamber inside the biosafety cabinet, push the sliding cover open with one hand (opening time approximately 1.5 seconds), confirm the target sample's numerical identifier through the transparent observation window, remove the sample with sterile forceps, and immediately close the sliding cover (single opening time ≤ 25 seconds). If continuous sampling is required, follow the "open-sampling-closing" cycle. Check the temperature display after every 5 samples. If it is ≥ -155℃, add 20mL of liquid nitrogen.

[0049] Subsequent processing: After the sampling is completed, transfer the remaining samples back to the liquid nitrogen tank, remove the detachable silicone holder and wipe it clean with sterile gauze to remove the frost layer; inject 30mL of liquid nitrogen into the adsorbent groove, close the side sliding cover and sealing plug, and place it in a dry and ventilated place for later use.

[0050] Example 3: Performance Testing and Results The performance of the low-temperature chamber described in Example 1 was tested under the following conditions: room temperature 25°C and relative humidity 50%. The results are as follows: Low temperature maintenance performance: Initial temperature -160℃, temperature drops to -158℃ after 4 hours when closed, and -152℃ after 6 hours; temperature rises to -156℃ after 30 seconds of opening the lid, and recovers to -160℃ after 5 minutes of closing.

[0051] Sampling efficiency: The sampling time for a single batch of 20 samples is 2 minutes and 38 seconds, which is 78.7% faster than the traditional liquid nitrogen tank direct sampling (12 minutes and 15 seconds); the total sampling time for 100 samples in 5 batches is 15 minutes and 42 seconds, which is 76.2% faster than the traditional method (65 minutes and 30 seconds).

[0052] Impact on sample viability: Using lung cancer organoid samples for testing, the survival rate after resuscitation following cryogenic preservation was 92.3%±1.5%, while the survival rate after preservation in a conventional liquid nitrogen tank was 79.1%±2.2%, representing a 16.7% increase in viability.

[0053] The above is a further detailed description of this utility model and should not be considered as a limitation on the specific implementation of this utility model. For those skilled in the art, simple deductions or substitutions without departing from the concept of this utility model are all within the protection scope of this utility model.

Claims

1. A low-temperature chamber specifically designed for rapid release of cell samples, characterized in that, include: The housing assembly is a double-layer structure, consisting of an outer shell (1), a vacuum insulation layer, and an inner liner nested in sequence. A refrigeration module is embedded in the bottom of the inner liner. The refrigeration module includes an adsorbent groove and a liquid nitrogen injection port. The adsorbent groove is filled with liquid nitrogen adsorbent material, and the liquid nitrogen injection port is located at the top of the box assembly and communicates with the adsorbent groove. Sample fixing module (2), wherein the sample fixing module (2) is attached and connected to the cooling module; A sealing cap assembly is movably connected to the top of the housing assembly to achieve opening and closing sealing.

2. The low-temperature chamber for rapid cell sample release according to claim 1, characterized in that, The outer shell (1) is made of stainless steel and has a thickness of 1.5-2mm; the inner liner is made of aluminum and has a thickness of 1-1.2mm; the vacuum insulation layer is made of vacuum glass wool and polyurethane composite and has a thickness of 3-4mm.

3. The low-temperature chamber for rapid cell sample release according to claim 1, characterized in that, The sample fixing module (2) is configured as a detachable silicone fixing frame. Multiple independent slots (21) are evenly distributed on the detachable silicone fixing frame. Each independent slot (21) is assigned a unique number. A cryopreservation tube can be inserted into each independent slot (21). The bottom of the detachable silicone fixing frame is provided with a positioning boss (22) that matches the adsorbent groove.

4. The low-temperature chamber for rapid cell sample release according to claim 3, characterized in that, The dimensions of the independent card slot (21) are 2.2cm×2.2cm×3.8cm, the dimensions of the cryopreservation tube are 1.8mL, and the center-to-center distance between adjacent independent card slots (21) is 3cm.

5. The low-temperature chamber for rapid cell sample release according to claim 1, characterized in that, The sealing cover assembly includes a sliding cover plate (31), a sealing ring (32), and a transparent observation window (33). The sliding cover plate (31) is slidably connected to the top of the box assembly via a slide rail. The sealing ring (32) is installed on the inner edge of the sliding cover plate (31). The transparent observation window (33) is made of low-temperature resistant glass and is fixedly installed in the middle of the sliding cover plate (31). The sliding cover plate (31) is provided with a liquid nitrogen pressure relief hole.

6. The low-temperature chamber for rapid cell sample release according to claim 1, characterized in that, It also includes a safety monitoring module, which includes a temperature sensor, a pressure sensor, an alarm light and a safety valve. The temperature sensor and the pressure sensor are respectively installed on the side wall of the inner liner and the box assembly. The temperature sensor is connected to a temperature display, which is fixedly installed on the outer shell (1). The alarm light and the safety valve are installed on the top of the box assembly.

7. The low-temperature chamber for rapid cell sample release according to claim 1, characterized in that, The side wall of the box assembly is provided with a frost-proof silicone handle (4), the bottom of the box assembly is provided with a non-slip rubber pad (5), and the liquid nitrogen injection port is connected with a sealing plug.

8. The low-temperature chamber for rapid cell sample release according to claim 1, characterized in that, The liquid nitrogen adsorption material is porous activated carbon, and the adsorbent groove is provided with a detachable cover plate. The detachable cover plate and the adsorbent groove are sealed together by a sealing gasket.

9. The low-temperature chamber for rapid cell sample release according to claim 1, characterized in that, The refrigeration module also includes a liquid nitrogen discharge port (6), which is located on the side wall of the housing assembly and communicates with the adsorbent groove.

10. The low-temperature chamber for rapid cell sample release according to claim 1, characterized in that, The external dimensions of the enclosure assembly are 28cm×18cm×8cm, and the effective volume of the inner liner is 25cm×15cm×4cm.

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