Method of determining volume dependent hypertension via reduction in phosphorylation

Abstract

A method of determining the presence of chronic volume dependent hypertension is provided-wherein a determination is made as to whether there has been a substantial reduction in phosphorylation of the blood-derived protein or renal proximal brush border membrane protein and if such reduction exists concluding that chronic volume dependent hypertension exists in a patient. The method may advantageously be practiced by employing blood serum or blood plasma as the body specimen containing the protein in determining whether a patient has chronic volume dependent hypertension, a cellular component of the blood, such as a blood-derived protein coming from the plasma membrane of lymphocytes. The method may include subsequent therapeutic patient treatment. Related diagnostic apparatus is also provided.

Description

[0001] 1. Field of the Invention

[0002] The present invention provides a means for determining whether a patient has volume dependent hypertension and, more specifically, it provides such a method based upon determining if a substantial reduction in phosphorylation of a specific protein exists. The invention also relates to a diagnostic apparatus employable in making such determination.

[0003] 2. Description of the Prior Art

[0004] Elevated blood pressure or hypertension has long been recognized as a health problem. It is a very common disease which can have widespread effects on a patient's body and frequently, unlike numerous other diseases, is asymptomatic.

[0005] Despite known means of measuring blood pressure of a patient as by a sphygmomanometer, for example, there is lacking an accurate reliable means of detecting the presence of volume dependent hypertension involving higher arterial blood pressure by use of a body specimen, such as blood serum or blood plasma.

[0006] From a path...

Examples

Embodiment Construction

[0031] In order to confirm the reliability of substantial reduction in phosphorylation of the blood-derived protein (Mr=72,000), experiments were performed on rats selecting two models of experimental hypertension which exemplify the two major modes of chronic essential hypertension (high blood pressure).

[0032] In the first model, the effects of chronic volume expansion employing DOCA-salt hypertension as discussed in Schenk, The Pathogenesis of DOCA-salt Hypertension, J. Pharmacol. Toxicol Methods, 27:pp. 161-170 (1992) was involved. The second model related to chronic vasoconstriction. The two-kidney, one clip (2K1C) model was prepared in accordance with the procedure of Huang, et al. Bilateral Renal Function Responses to Converting Enzyme Inhibitor (SQ 20, 881) in two-kidney, one clip Goldblatt Hypertensive Rats, Hypertension, 3:pp. 285-293 (1981). The experiments in connection with both types of hypertension were conducted with a view toward determining the effect of these types...

[0001] 1. Field of the Invention

[0002] The present invention provides a means for determining whether a patient has volume dependent hypertension and, more specifically, it provides such a method based upon determining if a substantial reduction in phosphorylation of a specific protein exists. The invention also relates to a diagnostic apparatus employable in making such determination.

[0003] 2. Description of the Prior Art

[0004] Elevated blood pressure or hypertension has long been recognized as a health problem. It is a very common disease which can have widespread effects on a patient's body and frequently, unlike numerous other diseases, is asymptomatic.

[0005] Despite known means of measuring blood pressure of a patient as by a sphygmomanometer, for example, there is lacking an accurate reliable means of detecting the presence of volume dependent hypertension involving higher arterial blood pressure by use of a body specimen, such as blood serum or blood plasma.

[0006] From a path...

Examples

Embodiment Construction

[0031] In order to confirm the reliability of substantial reduction in phosphorylation of the blood-derived protein (Mr=72,000), experiments were performed on rats selecting two models of experimental hypertension which exemplify the two major modes of chronic essential hypertension (high blood pressure).

[0032] In the first model, the effects of chronic volume expansion employing DOCA-salt hypertension as discussed in Schenk, The Pathogenesis of DOCA-salt Hypertension, J. Pharmacol. Toxicol Methods, 27:pp. 161-170 (1992) was involved. The second model related to chronic vasoconstriction. The two-kidney, one clip (2K1C) model was prepared in accordance with the procedure of Huang, et al. Bilateral Renal Function Responses to Converting Enzyme Inhibitor (SQ 20, 881) in two-kidney, one clip Goldblatt Hypertensive Rats, Hypertension, 3:pp. 285-293 (1981). The experiments in connection with both types of hypertension were conducted with a view toward determining the effect of these types...

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