The invention discloses an extraction method of leech
anticoagulant protein, and belongs to the technical field of biological extraction.According to the extraction method, after components of induction liquid are optimized, mucous membranes are activated, and the living tissue environment naturally sucked by leeches is simulated, so that the leeches can be induced to better suck, the sucking amount of the leeches is guaranteed, and the anti-coagulation
protein content of the leeches is improved; the leeches can be effectively stimulated to secrete more
anticoagulant proteins with higher activity; meanwhile, under the coordination effect of the
tranquilizer, the leech is kept in a warm state, so that the silent and relaxed physiological state of the leech can still be ensured in a dizziness stimulation or air
pressure difference stimulation state, the loss of the living leech can be reduced, conditions for repeated extraction are provided for the leech, the
gland secretion activity of the leech tends to be stable and vigorous, and the
survival rate of the leech is increased. The extraction rate is further improved.