35results about How to "Rich nitrogen source" patented technology

The invention relates to a cathode non-platinum catalyst of a proton exchange membrane fuel cell and a preparation method thereof. The method comprises the following steps: melamine-formaldehyde resin preparation reaction is carried out, and metal salt is added into the melamine formaldehyde resin; complexing reaction occurs between the melamine formaldehyde resin and the metal salt to form a complex; and after solvent of the complex is evaporated, the complex is decomposed by heat treatment to form the cathode non-platinum catalyst with a hollow spherical structure of the proton exchange membrane fuel cell. The non-platinum catalyst has following advantages that firstly the non-platinum catalyst has a large activity ratio surface, so that the oxygen reduction activity of a catalyst is greatly improved; secondly the catalyst has a rich nitrogen source; thirdly the catalyst has an excellent activity of oxygen reduction; and fourthly the preparation method of the non-platinum catalyst is simple, and cheap metals such as Fe, Co and the like are used as the catalyst, so that the synthesis cost is lowered.

The invention belongs to the field of soft soil foundation treatment, and relates to a biological cement reinforced roadbed on an under-consolidation silt blowing foundation and a construction method.Grille gravel piles in the roadbed penetrate through the under-consolidation silt blowing foundation and are embedded into a lower horizontal bearing layer. A plastic drainage plate is driven into the under-consolidation silt blowing foundation. A biological cement-grille reinforced cushion layer is flatly paved on the upper surface of the grille gravel piles. Non-woven geotextiles are laid on the upper surface and the lower surface of the biological cement-grille reinforced cushion layer. The non-woven geotextiles and a sealing film are sealed at the bottom of a tamped clay sealing ditch. Roadbed filling soil is packed on the upper surface of the biological cement-grille reinforced cushion layer and on the non-woven geotextile. Biological waterproof layers are arranged on the upper surface and the side surface of the roadbed filling soil. A soil layer is arranged on the biological waterproof layer on the roadbed side face, and slope protection vegetation is planted to form a roadbedslope ecological waterproof protection layer. The roadbed has the advantages of being reasonable in structure, high in bearing capacity, short in construction period, environmentally friendly and thelike, and is a green ecological structure worthy of popularization.

The invention provides a coprinus comatus culture medium which is composed of a dry material and water. The dry material is composed of the following components in parts by weight: 22-35 parts of ammonified celery leaf, 18-20 parts of kudzu root residue, 10-12 parts of peanut shell, 16-18 parts of bacterium residue, 8-10 parts of bran coat, 5-8 parts of garden soil, 1-2 parts of calcium fertilizer, 0.5-1 part of sulfur fertilizer, 3-5 parts of corn cob and 0.5-1 part of sugar. The weight ratio of the dry material to the water is 1:1.2. The invention also provides a preparation method of the coprinus comatus culture medium. The coprinus comatus cultured by the culture medium has higher yield and quality.

The invention relates to a new method for producing fuel ethanol by a low temperature pretreatment mode of corn. The method comprises the following steps: crushing corns into granules with granularity of 100%; sieving by a 40 mesh sieve; preparing a powder syrup whose dry material concentration is 33 m% with water and clear liquid; adding 15-20 U / g dry basis of acid protease and 30-40 U / g dry basis of alpha-amylase, adding 3 ppm of bacterial depressant; adjusting pH to 4.5; stirring at 60-65 DEG C for 0.5-1 hour; preparing a pretreatment mash; rapidly cooling the pretreatment mash to 30-35 DEG C; adding 0.2 m% of yeast and 400-500 U / g dry basis of complex glucoamylase, and adding nutritive salts; fermenting for 75-85 hours. Compared with the traditional high temperature boiling technology, the invention can save about 35% of steam consumption with high mature mash ethanol content and high starch liquor yield.

The invention provides a method for producing glycyrrhetinic acid through microbial fermentation transformation. The method comprises the following steps of: inoculating aspergillus flavus CGMCC No 5144 into a solid potato dextrose agar (PDA) slant culture medium to cultivate; inoculating 1 percent of the cultivated aspergillus flavus CGMCC No 5144 to a culture medium containing fresh seeds from the inclined surface to cultivate; inoculating 30 percent of the strains obtained from the seed culture medium to a fermentation tank containing a fresh fermentation culture medium and performing fermentation culture to obtain fermentation liquid containing the glycyrrhetinic acid; separating a glycyrrhetinic acid coarse product from the fermentation liquid by a membrane separation combined process; and preparing high-purity glycyrrhetinic acid by a purification process. In the glycyrrhetinic acid finally obtained by the method, the fermentation quantity reaches 4.38 g / L, the product purity is more than or equal to 98 percent, the conversion rate is more than or equal to 90 percent, and the technological scale level is reached.

The invention discloses a method for improving the yield of cordycepin and thermoduric proteinase of cordyceps militaris (L.) link through a liquid-state fermentation method, and relates to a strain which is preserved in China Committee for Culture Collection Center for general microbiology. By utilizing the strain, through preparation of cordyceps militaris (L.) link strain by liquid fermentation and production of cordycepin by liquid strain fermentation, an experiment result proves that the content of strain deep liquid-state ventilation fermentation cordycepin reaches 4.12g / L, and the activity of the thermoduric proteinase reaches 92554U / ml. Meanwhile, the method disclosed by the invention has the advantages of simple operation, low cost, short period, and easy realization; the raw materials are rich in sources and easy to get, are low in cost and have no pollution to the environment; the prices of the used equipment and reagents are cheap; and large-scale production is convenient.

The invention discloses a cordyceps active lactic acid bacteria beverage and a production method of the cordyceps active lactic acid bacteria beverage. The production method includes the steps that cordyceps bacteria are inoculated into a slant culture medium, then three-level liquid culturing is conducted, and the cordyceps active lactic acid bacteria beverage is obtained. According to the method, wort, egg white, fresh ginger and the like are added on the basis of a common medium, and the activity and the content of cordycepin, cordycepic acid and polysaccharide of the superoxide dismutase (SOD) in the beverage are increased remarkably; furthermore, the cordyceps beverage comprises active lactic acid bacteria; the original ecology and the original taste and flavor are kept, and no any antiseptic substance, preservative, antioxidants, thickener and other additives are added; the cordyceps active lactic acid bacteria beverage has special fresh flavor of the cordyceps beverage and tastes good.

The invention relates to compound microorganism fertilizer. The compound microorganism fertilizer is prepared from, by weight, 0.5 part of a compound microorganism bacterium agent, 25-30 parts of organic fertilizer, 1-2 parts of streptomycin bacterium residue extract, 20-60 parts of nitrogen phosphorous and potassium and 15-20 parts of soil conditioner. The compound microorganism bacterium agent includes saccharomycete, bacillus subtilis and bacillus licheniformis. The compound microorganism fertilizer is rich in nutrition, convenient to apply, high in valence, capable of utilizing waste resources efficiently and environmentally friendly, and saves energy.

The invention discloses a seed breeding method for high-yield and high-quality downward cluster pepper. The seed breeding method comprises the following steps of selection and breeding of a female parent: selecting a capsicum frutescens female parent of an upward cluster pepper variety and a Weiyuan seven star pepper male parent of upward cluster pepper variety to hybridize, planting, selfing, reserving seeds, selfing next generations, and repeating, wherein a variety which has optimum matching ability and of which the spicy degree meets the seed breeding requirement is selected, and is named as the capsicum frutescens female parent; selection and breeding of a male parent: selecting a xanthoxylum piperitum female parent of a downward cluster pepper variety and an IIN cluster downward pepper male parent to hybridize, planting, selfing, reserving seeds, selfing next generations, and repeating, wherein a variety which has optimum matching ability and of which the quality meets the seed breeding requirement is selected, and is named as the xanthoxylum piperitum male parent; hybridizing and grouping: hybridizing the capsicum frutescens female parent and the xanthoxylum piperitum male parent, planting, selfing, reserving seeds, and selfing next generations, wherein a variety which has optimum matching ability and of which the spicy degree, quality and oil content meet the requirements is selected, and is determined as downward cluster pepper; spraying a humic acid active liquid fertilizer to leaves of the downward cluster pepper, and planting, so as to obtain the high-yield and high-quality downward cluster pepper.

The invention discloses a method for preparing a carbon material from flocculated sludge and dye hazardous wastes, and a carbon material. The method comprises a: flocculating to precipitate printing and dyeing wastewater to obtain flocs, drying the flocs to obtain dry flocculated sludge, injecting the dyeing wastewater into a spray drying tower, carrying out drying to obtain dye hazardous waste powder, mixing the flocs and the dye hazardous waste powder, carrying out crushing, carrying out screening and carrying out vacuum treatment, b: carrying out pyrolysis on the flocs and the dye hazardouswastes, and c: carrying out ball milling on the black solids obtained by pyrolysis to obtain powder, adding deionized water into the powder, carrying out stirring, carrying out suction filtering through a plate-frame suction filter, repeatedly carrying out water washing and carrying out vacuum drying to obtain the carbon material. The flocculated sludge and dye hazardous waste in the treated industrial wastewater containing dye are used as pyrolysis precursors and the pyrolysis precursors are subjected to pyrolysis so that the nitrogen-doped carbon material is obtained. The method transformsthe flocculated sludge and hazardous wastes into the carbon material with a high added value, changes wastes into valuables and realizes environmental protection and economic income generation.

The invention belongs to the technical field of edible fungus production and cultivation, and discloses a needle mushroom multi-fruiting nutritional supplement solution, which is prepared from the components in parts by weight: 20 to 30 parts of monopotassium phosphate, 10 to 20 parts of calcium sulfate, 5 to 15 parts of magnesium sulfate, 20 to 30 parts of organic matter mixed liquor, 20 to 40 parts of nutrition additives, 2 to 6 parts of decavitamin, 1 to 5 parts of microelement, 3 to 8 parts of glucose, 3 to 10 parts of saccharose, 0.5 to 2 parts of corn germ oil, 1 to 5 parts of citric acid and 500 to 1000 parts of water. The nutritional supplement solution provided by the invention is specially prepared according to a growth characteristic of needle mushrooms, the needle mushrooms canbe promoted to fruit for the second time and even for multiple times by using the nutritional supplement solution provided by the invention, and comprehensive nutrition is provided for multi-fruitingof the needle mushrooms, so that the total yield of the needle mushrooms is improved, nutritional ingredients in a culture medium are fully utilized, environment waste is reduced, the feasibility ishigh, and the cultivation cost of the needle mushrooms is greatly reduced.

The invention discloses a method for raising colored pheasants by using paper mulberry tree leaves and relates to the field of chicken culture. The raising is divided into three stages, i.e., chickling raising, growing chicken raising and grown chicken raising, a feed formula adopted during the raising of each stage comprises a concentrated feed, fermented paper mulberry tree leaves and a premix, proportioning ratios of the concentrated feed to the fermented paper mulberry tree leaves to the premix in the three stages are all different, and the proportion of the fermented paper mulberry tree leaves gradually increases; and the fermented paper mulberry tree leaves are prepared through fermenting several kinds of microbes, i.e., streptomyces, bacillus licheniformis, aspergillus oryzae, Lactobacillus acidophilus and Streptococcus lactis with urea, sugar-cane juice, potassium chloride, magnesium sulfate and maize meal jointly. According to the method for raising the colored pheasants, disclosed by the invention, the quality of fermentation of the paper mulberry tree leaves is improved, and the quality of chicken is further improved; and the raising ratio of the concentrated feed to forage is reasonably prepared according to growth characteristics of the colored pheasants, so that the aims of saving the concentrated feed, reducing the cost and improving the economic benefit are achieved.

The invention belongs to the technical field of edible mushroom cultivation, and discloses a palm dreg containing hypsizygus marmoreus culture medium which comprises the following raw material components in parts by weight: 28-35 parts of bits of wood, 16-22 parts of corncob, 6-10 parts of bagasse, 18-26 parts of palm dreg, 5-8 parts of soybean meal, 0.8-1.5 parts of lime and 0.8-1.5 parts of gypsum powder. The hypsizygus marmoreus cultivated by using the culture medium is high in nutrient component content, short in growth cycle and simple and extensive in cultivation process, and labor can be saved.

The invention provides a microbial culture medium and a preparation method and application thereof. The microbial culture medium comprises the following components in parts by weight: 3.5-5.5 parts ofcottonseed meal, 0.8-1.4 parts of yeast powder, 4.5-8.5 parts of glucose, 4-5 parts of peptone, 0.05-0.1 part of sodium dihydrogen phosphate, 0.05-0.1 part of disodium hydrogen phosphate, 0.02-0.04 part of acetyl coenzyme A and 0.001-0.02 part of nitrate. By the provided microbial culture medium, absorption of microbes to nutrient substances and a related metabolic process can be promoted, stablegrowth and reproduction of the microbes are facilitated, and the microbial culture medium is excellent and is wide in applicability.

The invention relates to a one-pot method for simultaneously preparing carbon quantum dots and porous carbon. The method comprises the following steps: (1) weighing corncob lignin, adding phosphoric acid, adding deionized water until the corncob lignin is uniformly dispersed, transferring the mixture into a high-temperature high-pressure environment, keeping the temperature, stirring, and stoppingheating; (2) taking out the mixed solution, carrying out suction filtration to separate solid residues from the liquid, carrying out dialysis treatment on the obtained liquid, and carrying out rotaryevaporation and cold drying to obtain a nitrogen and phosphorus doped carbon quantum dot solid; and (3) carbonizing the collected solid residues in an inert gas atmosphere to obtain the porous carbondoped with nitrogen and phosphorus elements. According to the method, the carbon quantum dots with high fluorescence performance and the porous carbon material with large specific surface area and high conductivity are prepared by taking the corncob lignin as a raw material, the raw material is fully utilized, zero waste is realized, and the method has important significance for improving efficient and high-valued application of the lignin, solving energy crisis and protecting the environment.

The invention provides a culture medium for promoting the growth of morchella mycelia. For the culture medium, maltose is adopted as an optimal carbon source for culturing the morchella mycelia, ammonium tartrate is adopted as an optimal nitrogen source for culturing the morchella mycelia, high-purity chemical reagents with accurate ingredients are selected for preparation, so that the ingredients of the culture medium are accurate, and the quality control of the strains is facilitated. According to the nutrition ingredients required by the growth of the morchella mycelia, optimization is conducted, so that the growth speed of the morchella mycelia is accelerated, after inoculation is carried out for 7-9 days, the morchella mycelia grow on the whole inclined plane, the growth speed of the mycelia is improved by about 20% compared with the growth speed of the mycelia adopting a PDA culture medium, and the growth time of the morchella mycelia is shortened, in addition, the culture medium disclosed by the invention is simple in formula, wide in raw material source and low in cost, the production cost of the morchella can be reduced, and reliable technical support is provided for subsequent application of the morchella.

The invention discloses a shrimp-Chinese caterpillar fungus fermented live bacteria beverage and a preparation method thereof. The method comprises the following steps that Chinese caterpillar funguses are inoculated to a slope culture medium for cultivation, then the Chinese caterpillar funguses are inoculated to a liquid PDA enriched culture medium for two times of scale-up cultivation, and finally the Chinese caterpillar funguses are inoculated to a liquid fermentation culture medium for deep fermentation, and the shrimp-Chinese caterpillar fungus fermented live bacteria beverage is obtained. The liquid fermentation culture medium is composed of 10-20% of fresh shrimps, 0.5-3.0% of glucose, 0.5-3.0% of saccharose, 1-5% of fresh ginger, and the balance water. The pH value is natural. The fresh shrimps serve as the major component of the liquid fermentation culture medium and are rich in nutrition, excellent nutrient substance and environment conditions are provided for the Chinese caterpillar funguses, and besides, the health care effect and taste as well as flavor of the beverage are improved.

The invention relates to the technical field of preparation of crop fertilizers and provides a water-soluble fertilizer formula for soilless culture of giant pumpkins and a soilless culture method. The water-soluble fertilizer formula mainly comprises a base material and a nutrient solution, wherein the base material is prepared from raw materials including organic fertilizer, shredded coconut, mushroom dreg, bagasse, a water-retaining agent, datura powder, nerium oleander powder and the like; the nutrient solution is prepared from raw materials including calcium nitrate, ammonium dihydrogen phosphate, chelated iron, triacontanol, a penetrating agent, a microorganism preparation, a castor seed extracting solution, a tomato extracting solution, a daisy leaf extracting solution, a soybean root and leaf extracting solution, a cortex dictamni extracting solution, a linaria vulgaris extracting solution, a flos inulae extracting solution, an artemisia scoparia extracting solution, a carpesium cernuum extracting solution and the like; the soilless culture method comprises the culture steps of carrying out seedling culture management, carrying out field planting management, carrying out water-fertilizer management, remaining vines on whole branches, selecting fruits and positioning, carrying out disease and insect pest management. The formula of the base material and the nutrient solution is scientific and reasonable, and disease and insect pests and weed dangers are reduced; the absorption of crops on nutrient substances is promoted and the growth is rapid; the survival rate and the yield are improved and the formula is safe and environmentally friendly.

A method for producing organic fertilizer from soybean whey wastewater comprises steps as follows: after neutral salt is added to the soybean whey wastewater, the pH is adjusted to 4.0-5.8; (2) a wastewater liquor obtained in the step (1) is concentrated until the concentration is 30%-60%, and a concentrated liquor is obtained; (3) bacterial powder is added to the concentrated liquor obtained in the step (2), the obtained mixture is mixed with green manure plants, fermentation is performed, and the organic fertilizer is obtained. By means of the method, the problem about whey wastewater is solved, the whey wastewater is converted into the organic fertilizer, zero emission of sewage is realized, and besides, water resources are recycled.

The invention relates to an escherichia coli fermentation medium and a fermentation technology for producing succinic acid. A formula of the medium comprises 1.4-1.6g / dL corn steep liquor dry powder,0.5-2.5g / L yeast extract, 0.2-1.4g / L MgSO4, 2.5-4.5g / L (NH4)2HPO4, 0.6-2.0g / L C5H11NO2.HCl, 0.2-1.0g / L KCl, 50-60mg / L CuSO4, and 35-50mg / L FeSO4.7H2O; a pH of the medium is 6.5-6.8; and a required volume is reached by distilled water. The fermentation technology comprises the steps of taking escherichia coli as a fermentation strain, and performing aerobic enrichment in a primary seeding tank anda secondary seeding tank, and anaerobic fermentation in a fermentation tank to form succinic acid, wherein a formula of the fermentation medium is the above formula. According to the medium and the technology, the total fermentation cost can be lowered by 22%.

The invention discloses a method for preparing a carbon material from flocculated sludge and petroleum coke and the carbon material. The method comprises the following steps: a, drying flocs obtainedby flocculating and precipitating printing and dyeing wastewater to obtain dried flocculated sludge, mixing the dried flocculated sludge with the petroleum coke, and performing pulverizing, sieving and vacuum treatment; b, pyrolyzing the mixture of the vacuum treated dry flocculated sludge and petroleum coke; c, performing ball milling on a black solid obtained by pyrolyzing the mixture of flocs and petroleum coke into powder, adding the powder to a stirring kettle filled with deionized water for stirring, performing suction filtration by a plate-and-frame filter, performing washing repeatedlywith water until pH of a filtrate is 6-8, and finally performing vacuum drying to obtain the carbon material. The method and the carbon material have the beneficial effects that the petroleum coke and the flocculated sludge after treatment of industrial wastewater containing dye can be compounded as a pyrolysis precursor, and a nitrogen-doped carbon material is obtained by pyrolysis. Waste is turned into wealth, and accordingly, a balance between environmental protection and economic income generation is achieved.

The invention discloses a management method for improving quality of blueberry fruits. After being planted and cultivated, blueberry seedlings are managed. The management method includes processes ofirrigation, fertilization, pollination, laying of a reflective film, and soil management. The management method has the advantages that the reflective film is laid under a tree in a young fruit stageof blueberries to improve photosynthesis of the blueberries, and accordingly, accumulation of anthocyanin and vitamin C in the fruits is promoted; a bee pollination method is used, and accordingly, the fruiting rate and the weight of a single fruit are increased.

The invention relates to a special fertilizer for mulberry tree planting. The special fertilizer for mulberry tree planting is prepared from the following raw materials in parts by weight: 30-50 partsof dry manure powder, 30-40 parts of a potassium fertilizer, 30-40 parts of urea, 30-40 parts of tea leaf residues, 30-40 parts of monoammonium phosphate, 30-40 parts of ammonium chloride, 30-40 parts of sapropel, 30-40 parts of a bean cake fertilizer, 30-40 parts of silkworm excrement, 30-40 parts of peanut root powder, 10-20 parts of bee hive, 20-30 parts of soybean meal, 20-30 parts of herba andrographitis, 20-30 parts of minced garlic, 20-30 parts of trametes robiniophila, 20-30 parts of cordate houttuynia, 20-30 parts of honeysuckle, 20-30 parts of clove, 20-30 parts of radix isatidis, 16-26 parts of organosilicone, 2-5 parts of sodium cyclamate, seaweed 20-30 parts of algin and 1.8-2.2 parts of trace elements. The special fertilizer for mulberry tree planting has the characteristicsof lasting fertility, capabilities of promoting the healthy and strong growth of mulberry trees and enhancing the immunity and the like.

The invention discloses a green tea planting method in the technical field of green tea planting, wherein the method includes the following steps: step 1, reclamation; step 2, base fertilizer preparation: preparing the raw materials in parts by weight: 4-7 parts of distiller's grains, 8-11 parts of earthworm cast, 3-5 parts of sawdust, 4-6 parts of humus soil, 5-7 parts of cow manure and 1-2 partsof vermiculite; mixing the raw materials evenly, and pile-fermenting for 10-12 d in an environment of the temperature of 40-45 DEG C, to obtain a base fertilizer; step 3, nutrition solution preparation: preparing the raw materials in parts by weight: 1-2 parts of white sugar, 2-3 parts of rice vinegar, 10-15 parts of willow branches and 10-12 parts of water; and, mixing a juice obtained after squeezing the willow branches with white sugar, rice vinegar and water, to obtain a nutrient solution; step 4, tea seedling transplantation; and step 5, tea seedling management. Tea trees are planted bythe method, and the survival rate of tea seedlings can be effectively improved.

The invention belongs to the technical field of novel fertilizers, and particularly relates to a granular organic fertilizer prepared from sludge. The fertilizer is prepared from the following components in parts by weight: 46-60 parts of dried sludge, 10-12 parts of animal waste, 3-5 parts of an active microbial agent, 10-12 parts of rice hull powder, 7-9 parts of oxidized starch clay, 4-6 partsof durian shell-shaddock peel powder / allyl succinic anhydride / hyperbranched polyamidoamine (HBPNH2) and 6-10 parts of xanthan gum multi-grafted paraffin emulsion. The invention further provides a preparation method for preparing the granular organic fertilizer from the sludge. The granular organic fertilizer prepared from the sludge disclosed by the invention has remarkable technical effects of adsorbing heavy metals and coating a protective layer.