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Process for extracting epigoitrin from isatis root and use thereof

A technology that reports Yichun and Banlangen, applied in the direction of medical preparations containing active ingredients, pharmaceutical formulations, organic active ingredients, etc., can solve the problem of not being able to use large-scale production

Inactive Publication Date: 2007-10-10
SUZHOU SYLVAN NATURAL PROD R&D CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is only suitable for laboratory extraction, and its purpose is only for research use, and cannot be used for large-scale production due to cost and other reasons

Method used

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  • Process for extracting epigoitrin from isatis root and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Extraction of main parts

[0052] 1. Materials and Instruments

[0053] Materials: 100 g of isatidis extract prepared by Suzhou Siyuan Pharmaceutical Technology Co., Ltd. by the method of Chinese application 03110306.5.

[0054] Neutral alumina: purchased from Shanghai Ludu Chemical Reagent Factory

[0055] Silica gel H: Qingdao Ocean Chemical Co., Ltd.

[0056] 2. Extraction method:

[0057] Grind the Radix Radix Radix extract to 60 mesh; add it to the neutral alumina column; use chloroform:methanol mixed solvent gradient elution, the gradient is to increase 1ml methanol per 500ml eluent after collecting 50 fractions, the elution speed 6ml / min; collect the fractions containing epichun, and detect the concentration of epichun therein with HPLC; combine the fractions containing epichun; distill off the solvent under reduced pressure at 60°C.

[0058] Dissolve the obtained powder in methanol, add a small amount of silica gel H to the column of silica gel H, and wash w...

Embodiment 2

[0081] Anti-influenza virus experiment of extract

[0082] 1. Samples: a) indirubin, b) quinazolones, c) the epiblast prepared in experiment 3 of Example 1, with a concentration of 0.01 mg / ml, and d) prepared by the applicant according to the method of Chinese application 03110306.5 Radix isatidis extract, the concentration is 0.05mg / ml.

[0083] Control: ribavirin, concentration 0.01mg / ml.

[0084] 2. Detection method:

[0085] (1) Experimental materials:

[0086] Virus strain: influenza type I virus A / shanghai / 26 / 85 (H, N) strain, hemagglutination titer 1:1280 (preserved by the Department of Microbiology and Immunology, Nanjing Medical University).

[0087] Chicken embryos: A total of 22 10-year-old chicken embryos were hatched (Department of Microbiology and Immunology, Nanjing Medical University).

[0088] Chicken erythrocytes: adult males were washed three times with normal saline to prepare a concentration of 0.5%.

[0089] (2) Experimental method:

[0090] a) Grou...

Embodiment 3

[0097] Cytopathic Research

[0098] 1. Test material:

[0099] Sample: Table Yichun prepared in Example 1 Experiment 3

[0100] Virus strain: Influenza virus A / FM1 / 47 / H1N1 (sourced from Department of Microbiology and Immunology, Nanjing Medical University).

[0101] Sendai virus (gifted by the Department of Microbiology and Immunology, Nanjing Medical University).

[0102] Cells: MDCK cells (gifted by the Department of Microbiology and Immunology, Nanjing Medical University).

[0103] 2. Experimental method:

[0104] a) Administer 0.6 μg / ml epiblaston to the cells, and add 100 TCID after 24 hours 50 influenza virus or Sendai virus.

[0105] b) The sample was mixed with 0.6μg / ml and 100TCID 50 Influenza virus or Sendai virus was mixed, placed overnight at 4°C, and the cells were inoculated the next day.

[0106] c) The sample was directly mixed with 0.6μg / ml and 100TCID 50 Influenza virus or Sendai virus and cell mix.

[0107] After 24-48 hours, the cytopathic ch...

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Abstract

This invention discloses a method of extracting biao-gao-yi-chun from isatis root. The procedures include (a) ethanol water solution is back flow extracting solution and its weight is 30-95% of 6-12 times isatis root weight, then extracting solutions are combined. (b) Ethanol is recovered after filtration and then liquor is got. (c) The liquor flows through big holes resin adsorption column. (d) Scrubbing column is scrubbed by scrub solution until effluent liquid is leuko. (e) It is eluted by 30-95% weight percentage ethanol water solution, and eluent is collected. (f) The eluent is dried and solid substance is got. (g) Average particle diameter is -0.001-2mm powder is got after grinding. (h) The powder got from (g) is added to alumina column, (i) gradient elution is done by chloroform and ethanol component solvent, every 200-1000ml is one flow portion in order to add 0.1-2ml methanol after collecting every 50 portion. (j) The portions contain biao-gao-yi-chun are collected. (k) Solvent is discharged from the portion in (j), and then biao-gao-yi-chun extract product is got. This invention also provides the use of biao-gao-yi-chun in preparing antivirus medicine.

Description

technical field [0001] The invention relates to the field of extraction technology of effective components of traditional Chinese medicines, in particular, it relates to the extraction and preparation of Radix Radix Radix Isatidis Zhongbiaoyichun. The present invention also relates to the new medicinal application of Yichun. Background technique [0002] Banlangen is a commonly used Chinese herbal medicine, which is the root of Isatis indigotica. It is widely cultivated in China and has the effect of clearing away heat and detoxifying (Zhonghua Materia Medica, pp.2346, 3.709). Existing reports show that it has antiviral (Isatis Radix Chemical Constituents Research (I), Chinese Herbal Medicine, Volume 32, No. 12, 2001, pp1057), antibacterial endotoxin (Herald of Medicine, Volume 21, No.2, pp. 74, 2002), anticancer, antidepressant (Chinese herbal medicine, 2001, Vol. 32, No. 7, pp.670-671). It is also published in literature that the antiviral effect of Radix isatidis is de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D263/16A61K31/421A61P31/12A61P31/16
Inventor 徐丽华
Owner SUZHOU SYLVAN NATURAL PROD R&D CO LTD
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