Application of chitosan in preparing drugs for preventing and curing bacterial septicemia of silkworm

A chitosan and bacterial technology is applied in the application field of chitosan in the preparation of medicines for preventing and treating bacterial septicemia of silkworm, which can solve the problems of inconspicuous, time-consuming and labor-intensive bacterial control effect and high cost, and reduce the risk of infection. , the treatment effect is remarkable, the effect of low cost

Inactive Publication Date: 2009-11-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology helps stop or treat serious illnesses caused by harmful microorganisms that cause blood poisoning called Silworm's disease. It makes it easier for people who are sick with these diseases to get rid of their symptoms quickly without putting them at greater danger than they should be doing otherwise.

Problems solved by technology

This patented technical problem addressed in this patents relates to reducing or controlling diseases associated with plants that require frequent use of pesticides due to lack of effective treatments against harmful microorganisms such as germinary tract illnesses like berger' s syndrome.

Method used

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  • Application of chitosan in preparing drugs for preventing and curing bacterial septicemia of silkworm
  • Application of chitosan in preparing drugs for preventing and curing bacterial septicemia of silkworm
  • Application of chitosan in preparing drugs for preventing and curing bacterial septicemia of silkworm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Dissolve chitosan powder with a deacetylation degree greater than 85% in 1% glacial acetic acid by volume, add distilled water to prepare chitosan solutions with concentrations of 0.01, 0.05 and 0.10 mg / mL, and take various concentrations of chitosan Chitosan solution 5mL, add the Bleeding Bacteria septicemia suspension of same concentration in every portion, make the concentration of bacteria in the mixed solution consistent (1.0×10 7 CFU / mL); use distilled water instead of chitosan powder as a contrast, check the number of germs after shaking at room temperature for 2 hours, the results are as follows:

[0015] The influence of table 1 different concentration chitosan solution on the growth of bleeding bacteria pathogen

[0016]

[0017] As can be seen from Table 1, under in vitro conditions, chitosan solutions of 0.01 mg / mL and 0.05 mg / mL can completely inhibit the proliferation of pathogenic bacteria, and chitosan solutions of 0.10 mg / mL can even kill existing ba...

Embodiment 2

[0019] Take the chitosan solution whose concentration is 0.10mg / mL, add a certain concentration of bleeding bacteria septicemia suspension, so that the concentration of bacteria in the solution is about 10 7 CFU / mL (7.00log 10 CFU / mL), the mixed solution was sampled and counted at 0.5, 1.0, 2.0 and 4.0 hours after mixing, and the number of bleeding and septicemia bacteria therein was recorded. Chitosan was replaced by distilled water as a control. The results showed that after chitosan was added to the germ suspension, the number of germs decreased sharply, showing a better bactericidal effect.

[0020] The influence of table 2 different culture time on chitosan solution (0.10mg / mL) antibacterial activity

[0021]

Embodiment 3

[0023] Soak mulberry leaves with a chitosan solution with a concentration of 0.10 mg / mL, feed the silkworms for 24 hours, inoculate the silkworms with Bleeding bacteria and septicemia, and use the following five treatments as controls: mulberry leaves were fed to silkworms, and silkworms were inoculated with pathogenic bacteria; ② mulberry leaves that had not been treated with chitosan were fed to silkworms, and silkworms were inoculated with distilled water; ③ Silkworms were fed with mulberry leaves treated with chitosan, and silkworms were inoculated with distilled water; ④ The silkworms were inoculated with distilled water; The mulberry leaves treated with chitosan were fed to silkworms, and silkworms were not inoculated; ⑤ The silkworms were fed with mulberry leaves treated with chitosan, and silkworms were not inoculated. Mortality of silkworms were counted at 24, 48 and 72 hours after inoculation.

[0024] Table 3 Chitosan solution treatment of mulberry leaf control effe...

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Abstract

The invention discloses an application of chitosan in preparing drugs for preventing and curing bacterial septicemia of silkworm. The drug can obviously reduce the risk of infection of the bacterial septicemia of silkworm and has obvious curative effect. The adoption of the drug for preventing and curing bacterial septicemia of silkworm is easy to operate and has lower cost; and more importantly, the preventing and curing links can not generate any threat to the health of workers working for preventing and curing.

Description

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Claims

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Application Information

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Owner ZHEJIANG UNIV
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