Antiphlogistic medicinal composition and preparation method thereof
A technology of anti-inflammatory drugs and compositions, applied in the field of traditional Chinese medicine preparations, to achieve the effects of improving drug efficacy, simplifying material composition, and reducing dosage
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Embodiment 1
[0021] Take mussel meat, wash it, mince it, add three times the amount of water to decoct and extract 3 times, each time for 30 minutes, combine and collect the decoction liquid, concentrate under reduced pressure to 1 / 9 of the total volume, add 95% ethanol until the alcohol volume is 100 The fraction content was 80%, centrifuged at 6000rmp for 10min, discarded the precipitate and collected the supernatant, concentrated under reduced pressure and evaporated ethanol to obtain the total extract part. The total extract is dissolved with an appropriate amount of water, the resulting solution is extracted with an equal volume of ethyl acetate / petroleum ether mixed solvent, and the remaining solution is extracted with an equal volume of n-butanol to obtain n-butanol extract, and n-butanol is recovered to obtain n-butanol Extract the extract from the alcohol part and dry it to obtain the total extraction part of nucleoside alkaloids.
Embodiment 2
[0023] Take mussel meat, wash it, mince it, add three times the amount of water and ultrasonically extract it for 3 times, each time for 30 minutes, combine and collect the decoction liquid, concentrate it under reduced pressure to 1 / 9 of the total volume, add 95% ethanol to the volume percentage of alcohol The content is 80%, centrifuged at 6000rmp for 10min, discarding the precipitate to collect the supernatant, concentrating under reduced pressure and evaporating ethanol to obtain the total extract part, the total extract is dissolved with an appropriate amount of water, and the obtained solution is mixed with an equal volume of ethyl acetate / petroleum ether Solvent extraction, extracting the remaining solution with an equal volume of n-butanol to obtain n-butanol extract, recovering n-butanol to obtain n-butanol extract, drying to obtain the total extraction fraction of nucleoside alkaloids.
Embodiment 3
[0025] Take mussel meat, wash, mince, freeze-dry, and pulverize. The powder is extracted with 80% ethanol under reflux. The extract is centrifuged to get the supernatant, and the solvent is evaporated to obtain the alcohol extraction extract. The extract is dissolved in an appropriate amount of water to obtain the total extract. site, the total extract is dissolved with an appropriate amount of water, the resulting solution is extracted with an equal volume of ethyl acetate / petroleum ether mixed solvent, and the remaining solution is extracted with an equal volume of n-butanol to obtain an n-butanol extract, and the n-butanol is recovered to obtain The extract from the n-butanol part is dried to obtain the total extraction part of nucleoside alkaloids.
[0026] (2) Compositional analysis of nucleoside alkaloids
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