Rapid seedling culturing method of pinus sylvestris in tube

Abstract

The invention relates to plant propagation technology, in particular to a rapid seedling culturing method of a pinus sylvestris in a tube. The method comprises the following steps: (1), a branch of a trunk of a wild pinus sylvestris plant is cultured in water, the stem section and stem tip are remained for spare use as explants after processed; (2), 0.07 mg / L to 0.08 mg / L -1 of indolebutyric acid, 0.03 mg / L to 0.04 mg / L -1 of naphthylacetic acid, 0.16 mg / L to 0.17 mg / L -1 of zeatin and 2.90 mg / L to 3.20 mg / L -1 of gibberellin are added to a minimal medium; and (3), rapid propagation, seedling hardening and transplanting are conducted to the pinus sylvestris plant. The main stem section of the wild pinus sylvestris plant is taken as the materials, the main stem section is controlled to take root in the tube and meanwhile a main stem bud germinates and grows so that single-step rapid propagation purposes of stem proliferation are achieved, the problems that seeds are difficult to propagate, cutting propagation root taking rate and survival rate of transplanting are low, wild resources receive extinctive threatens and the like are solved, and high quality seedlings are provided for further manual large-scale propagation and pinus sylvestris culturing.

Description

[0001] technical field

[0002] The invention relates to a plant propagation technology, that is, a method for quickly growing seedlings of Changbai pine in a test tube. Background technique

[0003] In the prior art, long white pine [ Pinus sylvestris var. sylvestriformis (Takenouchi) Cheng et C. D. Chu], also known as the beautiful pine, is an evergreen tree in the family Pinaceae and is a unique species of Changbai pine. This species is rare in the Changbai Mountains, and its distribution area is very narrow. It is only concentrated in the Changbai Mountains National Nature Reserve (more than 100 hm 2 ) and Erdaobaihe Town, Antu County (more than 100 hm 2 ), the rest are scattered in the coniferous and broad-leaved mixed forests and coniferous forests along the Erdaobai River and Sandaobai River. It is designated as an endangered species by the "List of Rare and Endangered Plants in China (Volume 1)", and it is a national third-level key protected plant. It is design...

Examples

Embodiment Construction

[0016] A kind of rapid seedling raising method in test tube of Changbai pine:

[0017] 1 Materials and methods

[0018] 1.1 Materials and handling

[0019] In the first ten days of November, take the trunk branches of wild Changbai pine plants, put them in a freezer at -10-0°C (to ensure that the buds are fully dormant) and dormant for 50 days. 4.0 cm, cut off the trunk and cut into segments (1.5 cm in length), remove the young needles and short petioles, soak in 5% sodium carbonate solution for 3 min to remove surface oil, 75% ethanol (volume fraction) Rinse for 30 s, move to saturated sodium hypochlorite solution and soak for 8 min, rinse with sterile water for 6 times, blot the surface moisture with sterile filter paper, remove the tissue poisoned by ethanol and sodium hypochlorite, and leave 1.0 cm of stem segment and shoot tip as The explants were spared.

[0020] 1.2 Stem rooting of Changbai pine and terminal bud germination and growth at the same time, rapid planting...

[0001] technical field

[0002] The invention relates to a plant propagation technology, that is, a method for quickly growing seedlings of Changbai pine in a test tube. Background technique

[0003] In the prior art, long white pine [ Pinus sylvestris var. sylvestriformis (Takenouchi) Cheng et C. D. Chu], also known as the beautiful pine, is an evergreen tree in the family Pinaceae and is a unique species of Changbai pine. This species is rare in the Changbai Mountains, and its distribution area is very narrow. It is only concentrated in the Changbai Mountains National Nature Reserve (more than 100 hm 2 ) and Erdaobaihe Town, Antu County (more than 100 hm 2 ), the rest are scattered in the coniferous and broad-leaved mixed forests and coniferous forests along the Erdaobai River and Sandaobai River. It is designated as an endangered species by the "List of Rare and Endangered Plants in China (Volume 1)", and it is a national third-level key protected plant. It is design...

Examples

Embodiment Construction

[0016] A kind of rapid seedling raising method in test tube of Changbai pine:

[0017] 1 Materials and methods

[0018] 1.1 Materials and handling

[0019] In the first ten days of November, take the trunk branches of wild Changbai pine plants, put them in a freezer at -10-0°C (to ensure that the buds are fully dormant) and dormant for 50 days. 4.0 cm, cut off the trunk and cut into segments (1.5 cm in length), remove the young needles and short petioles, soak in 5% sodium carbonate solution for 3 min to remove surface oil, 75% ethanol (volume fraction) Rinse for 30 s, move to saturated sodium hypochlorite solution and soak for 8 min, rinse with sterile water for 6 times, blot the surface moisture with sterile filter paper, remove the tissue poisoned by ethanol and sodium hypochlorite, and leave 1.0 cm of stem segment and shoot tip as The explants were spared.

[0020] 1.2 Stem rooting of Changbai pine and terminal bud germination and growth at the same time, rapid planting...

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