Building method of oxidation stress model of porcine circovirus 2 type in-vitro infection mouse mononuclear macrophages
A mononuclear macrophage and porcine circovirus technology, applied in biochemical equipment and methods, animal cells, vertebrate cells, etc., can solve problems such as lack of understanding and changes in the redox state of immune cells
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[0026] 1. Research ideas
[0027] Mouse mononuclear macrophage cell line (RAW264.7 cells) was infected by PCV2, and the levels of nitric oxide (NO) secreted by infected cells, total intracellular reactive oxygen species (ROS) levels, and reduced glutathione (GSH) were measured Content, xanthine oxidase (XOD) activity, myeloperoxidase (MPO) activity, inducible nitric oxide synthase (iNOS) activity, to explore the relationship between PCV2 virus infection amount, infection time and dynamic changes of reactive oxygen species level and establish an in vitro model of oxidative stress in mouse monocyte-macrophages.
[0028] 2. Experimental method
[0029] (1) Cultivation of RAW264.7 cells: RAW264.7 cells were resuscitated and transferred to bottles with DMEM culture solution containing 10% fetal bovine serum, and incubated at 37°C and 5% CO 2 cultured in an incubator. Subculture when the cells grow to 70%-80%, digest with 0.05% trypsin and then subculture at a ratio of 1:3. After...
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