Handheld electroporation device

An electroporation, hand-held technology, applied in the direction of biochemical instruments, biochemical equipment and methods, stress-stimulated microbial growth methods, etc., can solve the problems of inconvenient operation, cumbersome operation, and bulky electroporation equipment, and achieve fast and efficient use , Ease of use

Active Publication Date: 2017-08-18
TSINGHUA UNIV
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

[0002] The traditional electroporation equipment is bulky and inconvenient to operate. The electroporation equipment and the electroporation cup are independent of each other. The electroporation equipment does not have the functions of pipetting and electroporation at the same time.
In the electroporation experiment, it is first necessary to mix the cells and foreign substances in the EP tube, then draw the suspension containing cells and foreign substances into the electroporation cup for electroporation, and then transfer the electroporated suspension Then suck it into the petri dish, the operation is extremely cumbersome

Method used

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  • Handheld electroporation device
  • Handheld electroporation device
  • Handheld electroporation device

Examples

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Embodiment Construction

[0023] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals designate the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary and are intended to explain the present invention and should not be construed as limiting the present invention.

[0024] Refer below Figure 1-Figure 3 A handheld electroporation device 100 according to an embodiment of the present invention is described. The handheld electroporator generally includes: a housing 10 and a gun tip assembly 40 .

[0025] Specifically, such as figure 2 As shown, the housing 10 defines an airtight accommodation chamber, in which an electric pulse unit 20 and a sampling unit 30 are sequentially arranged, and the housing 10 is provided with a mounting hole 11, which communicates with the airtight accommodation c...

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Abstract

The invention discloses a handheld electroporation device, which comprises a shell and a head assembly, wherein a closed accommodating cavity is defined in the shell; an electric pulse unit and a sampling unit are sequentially arranged in the closed accommodating cavity; an installation hole is formed in the shell and communicates with the closed accommodating cavity; the head assembly is connected with the installation hole and comprises a head; a liquid storage cavity is defined in the head, is used for storing a suspension liquid containing cells and exogenous substances and is provided with a gas inlet and outlet and a liquid inlet and outlet; the sampling unit communicates with the gas inlet and outlet; the head assembly is electrically connected with the electric pulse unit; and the liquid storage cavity generates an electric field to carry out electroporation on cells through an electric pulse generated by the electric pulse unit and the exogenous substances are introduced into the cells. The handheld electroporation device is convenient to use, and is fast and efficient.

Description

technical field [0001] The invention belongs to the technical field of cell transfection equipment, and in particular relates to a hand-held electroporation device. Background technique [0002] The traditional electroporation equipment is bulky and inconvenient to operate. The electroporation equipment and the electroporation cup are independent of each other. The electroporation equipment does not have the functions of pipetting and electroporation at the same time. In the electroporation experiment, it is first necessary to mix the cells and foreign substances in the EP tube, then draw the suspension containing cells and foreign substances into the electroporation cup for electroporation, and then transfer the electroporated suspension Then suck it into the petri dish, the operation is extremely cumbersome. Contents of the invention [0003] The present invention aims to solve one of the above-mentioned technical problems at least to a certain extent. [0004] For thi...

Claims

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Application Information

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IPC IPC(8): C12M1/42
CPCC12M35/02
Inventor徐友春程振苏世圣
OwnerTSINGHUA UNIV