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Peptide having neuron loss prevention and regeneration effects and composition comprising same

A composition and an effective amount of technology are applied in the field of peptides with neuron loss prevention and regeneration effects and compositions containing the peptides, which can solve the problems that the correlation of human brain dysfunction is not clearly explained, and achieve the prevention of neuron loss. effect of loss

Active Publication Date: 2021-09-07
GEMVAX & KAEL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0021] As mentioned above, insoluble fibrillar aggregates of β-amyloid are representative markers of neuropathology in AD, but their relevance to human brain dysfunction is not clearly explained

Method used

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  • Peptide having neuron loss prevention and regeneration effects and composition comprising same
  • Peptide having neuron loss prevention and regeneration effects and composition comprising same
  • Peptide having neuron loss prevention and regeneration effects and composition comprising same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0137] Example 1: Peptide Synthesis

[0138] 1. Peptide Synthesis

[0139] The peptide of SEQ ID NO: 1 (hereinafter referred to as "PEP1") was prepared according to a conventionally known solid-phase peptide synthesis method. Specifically, peptides were synthesized by coupling each amino acid to another amino acid from the C-terminus by Fmoc solid-phase peptide synthesis (SPPS) using ASP48S (Peptron, Inc., Daejeon, Korea). Use a peptide where the first amino acid at the C-terminus is attached to the resin:

[0140] NH 2 -Lys(Boc)-2-chloro-trityl resin

[0141] NH 2 -Ala-2-chloro-trityl resin

[0142] NH 2 -Arg(Pbf)-2-chloro-trityl resin

[0143]In all amino acid compositions used for peptide synthesis, the N-terminus was protected with Fmoc, and the residues were protected with Trt, Boc, tert-butyl ester (t-Bu) and 2,2,4,6,7-pentamethyldihydro - Benzofuran-5-sulfonyl (Pbf) (which can be removed in acid) protection. Examples of amino acid compositions are as follows: ...

Embodiment 2

[0157] Embodiment 2: experimental preparation and material

Embodiment 2-1

[0158] Embodiment 2-1: the cultivation and processing of NSC and primary cortical nerve cells

[0159] All procedures involving animals were performed in accordance with the guidelines of the Animal Care and Use Committee of Hanyang University. All experiments were performed using a minimum number of animals with minimal pain, and all animals were used only once in an experiment.

[0160] NSCs were isolated from the brains of rat embryos, cultured and developed. NSC culture was performed in the same manner as will be described below. Briefly, rat embryos were obtained at embryonic day 13, and brains were rapidly removed and placed in petri dishes containing cold phosphate-buffered saline (Hank's balanced salt solution; HBSS).

[0161] Single cells were isolated from the cerebral cortex, lateral ganglionic eminence, and embryonic midbrain. Place these cells in culture dishes pretreated with L-ornithine and fibronectin containing phosphate-buffered saline (PBS) (without calci...

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Abstract

The present invention relates to compositions for the prevention and regeneration of neurons, and more particularly to compositions for the prevention and regeneration of neurons comprising telomerase-derived peptides for the treatment of neuronal loss and inability to regenerate Known diseases are effective. According to the present invention, the peptide shows preventive and therapeutic effects on diseases of neuron loss and regenerative incapacity including Alzheimer's disease, and thus can provide a new method for treating neuron loss and regenerative incapacity diseases.

Description

technical field [0001] The present invention relates to peptides effective in neuronal regeneration and prevention of neuronal loss and compositions comprising the same, and more particularly to compositions for the prevention and treatment of cerebrovascular or neurodegenerative diseases comprising telomerase-derived peptides , and is effective for neuron regeneration and prevention of neuron loss, thereby preventing and treating diseases caused by neuron loss. Background technique [0002] Neuron cells are various types of cells differentiated from neural stem cells (NSC). NSCs differentiate into various types that make up the nervous system, such as neurons, glial cells, astrocytes, and oligodendrocytes. [0003] There are markers indicative of the various steps of NSC differentiation into neurons, including: proliferation, differentiation, migration, targeting, and synaptogenesis. Doublecortin (DCX) is a protein expressed in neural progenitor cells and indicates immatu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08A61K38/10
CPCA61K38/10A61K45/06A61P25/16A61P9/10A61P25/28A61P25/14A61P25/02A61K2300/00C07K7/08C12Y207/07049
Inventor 高成昊李圭庸金商在
Owner GEMVAX & KAEL
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