Gdsl-type esterase/lipase marker gene associated with early ripening in grape

A marker gene, lipase technology, applied in the field of biology

Active Publication Date: 2021-08-27
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Two GELP genes involved in fruit development and ripening were reported in peach, but the GELP genes related to fruit development in grape have not been reported

Method used

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  • Gdsl-type esterase/lipase marker gene associated with early ripening in grape
  • Gdsl-type esterase/lipase marker gene associated with early ripening in grape
  • Gdsl-type esterase/lipase marker gene associated with early ripening in grape

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Embodiment Construction

[0033] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention.

[0034] Such as Figure 1-4 Shown, the present invention provides a kind of technical scheme: grape prematurity relevant GDSL type esterase / lipase (GELP) mark gene, comprises the steps:

[0035]S1, select the control group: select naturally grown "Fengzao" grapes and "Kyoho" grapes treated with distilled water, wherein the distilled water contains 0.03% silicon-wet-77 surfactant;

[0036] S2, treatment of experimental group: 300mmol / L of H 2 o 2 Evenly spray on the "Kyoho" grapes, wherein, the first spraying is 25 days after flowering, the second time is 35 days after flowering, each process is repeated three times, and each time repeats five trees;

[0037] S3. Collecting materials: each sampling starts at 9:00 a.m., wraps them in tin foil, and immediately puts them into a ...

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Abstract

The present invention discloses a GDSL-type esterase / lipase marker gene related to early maturation of grapes, comprising the following steps: S1, selecting a control group: selecting naturally grown "Feng Zao" grapes and "Kyoho" grapes treated with distilled water; S2, experimenting Group treatment: 300mmol / L of H 2 o 2 Evenly spray on the "Kyoho" grapes, wherein the first spraying is 25 days after flowering, the second time is 35 days after flowering, each process is repeated three times, and five trees are repeated each time. In the present invention, real-time fluorescence quantitative processing is performed on post-precocious "Kyoho" grapes treated with hydrogen peroxide and untreated "Fengzao" grapes, and candidate VvGELP genes related to grape fruit development are screened, in order to deeply understand GDSL esters in grapes. The function of the enzyme / lipase gene family and the in-depth study on grape early ripening provide a theoretical basis.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a GDSL-type esterase / lipase marker gene related to early ripening of grapes. Background technique [0002] Grape is one of the most widely cultivated fruit trees in the world. Grape is a worldwide fruit with a wide range of uses. It is not only used in large quantities for fresh food, but also used to brew different types of wine and processed into raisins and grape juice. Early-maturing grapes are an important attribute of capsule crops. The development of early-maturing grape production promotes the early release of grapes, which not only caters to consumer preferences, but also improves the economic benefits of grapes and promotes the development of agricultural production. Therefore, it is of great significance to study the early ripening mechanism of grapes. The new early-maturing grape variety "Fengzao" is a bud change of "Kyoho". The ear of this variety is conical and medium-l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6851
CPCC12Q1/6851C12Q1/6895C12Q2600/158C12Q2531/113C12Q2563/107C12Q2561/113C12Q2537/165
Inventor 郭大龙余义和郭丽丽张国海郑玉萍尼沛艺姬晓汝
Owner HENAN UNIV OF SCI & TECH
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