Gdsl-type esterase/lipase marker gene associated with early ripening in grape
A marker gene, lipase technology, applied in the field of biology
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[0033] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention.
[0034] Such as Figure 1-4 Shown, the present invention provides a kind of technical scheme: grape prematurity relevant GDSL type esterase / lipase (GELP) mark gene, comprises the steps:
[0035]S1, select the control group: select naturally grown "Fengzao" grapes and "Kyoho" grapes treated with distilled water, wherein the distilled water contains 0.03% silicon-wet-77 surfactant;
[0036] S2, treatment of experimental group: 300mmol / L of H 2 o 2 Evenly spray on the "Kyoho" grapes, wherein, the first spraying is 25 days after flowering, the second time is 35 days after flowering, each process is repeated three times, and each time repeats five trees;
[0037] S3. Collecting materials: each sampling starts at 9:00 a.m., wraps them in tin foil, and immediately puts them into a ...
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