Long-acting boar semen diluent powder and preparation method thereof

Abstract

The present invention relates to the technical field of pig breeding, and in particular to a long-acting boar semen diluent and a preparation method thereof. The present invention has four main components, namely, a nutrient, a buffer, a Ph regulator and an antibacterial agent, as well as two components, namely, a nutrient regulator and an osmotic pressure regulator, which improves the activity and survival time of sperm in the pig semen diluent, and allows the pig semen diluted by the pig semen diluent to be stored for 9-20 days at a room temperature of 3-30°C. At the same time, the product solves the disadvantage that the pig semen is slow to warm up after being stored at 4-20 degrees, and the warming up time is increased by 30%-50%. At the same time, the problem of too fast precipitation is solved. The formula can slow down the precipitation speed by 80%, and the storage process does not need to be manually shaken every 24 hours, and the storage is not affected.

Description

Technical Field

[0001] The invention relates to the technical field of pig reproduction, in particular to a long-acting boar semen diluting powder and a preparation method thereof. Background Art

[0002] Artificial insemination technology for pigs is a breeding method that uses artificial instruments to collect boar semen, inspect the semen quality and dilute it, and then inject the semen into the reproductive tract of estrus sows to replace natural mating. It quickly became popular around the world in the 1980s. At present, the penetration rate has exceeded 80% in Europe and North America, where it is widely used. It is an important means of scientific pig farming and realizing the modernization of pig production. It has the following advantages: (1) It saves boars and reduces the number of times each boar is used, overcoming time and regional differences and allowing for convenient and timely breeding; (2) It saves manpower, material and financial resources, improves economic benefits and reduces the spread of diseases; (3) It improves the breeding rate. Since the quality of sperm can be monitored by microscopes, density meters and other instruments, the breeding conception rate is effectively improved, and boars with poor sperm quality can be adjusted in time; (4) It increases the utilization rate of boars with excellent genetic performance, expands the coverage rate of good breeds, and is conducive to variety improvement and joint breeding.

[0003] When carrying out artificial insemination of pigs, a diluent for boar semen must be prepared for dilution and preservation.

[0004] Chinese patent document CN103518703A discloses a pig semen diluent, which contains glucose, trisodium citrate, potassium chloride, polyvinyl alcohol, trimethylolmethane, citric acid, ciprofloxacin, glutamine dipeptide and antifreeze protein. Using the diluent of the present invention, it is tested that the sperm motility reaches 0.87%, the sperm acrosome integrity rate reaches 91.2%, and the sperm deformity rate is as low as 7.67%, which can effectively improve the sperm retention time, but the pig semen diluent can only store semen for 3 days, and the time required for the pig semen diluent to store semen after 4-20 degrees of temperature storage and then warming up is very slow, and it is easy to precipitate. At the same time, it needs to be manually shaken every 24 hours during the storage process to prevent shock and death, and the operation is relatively cumbersome. Summary of the invention

[0005] In order to solve the above problems, the present invention provides a long-acting boar semen diluent powder and a preparation method thereof. At the same time, the product solves the disadvantage that the boar semen is slow to return to temperature after being stored at 4-20 degrees, and the return to temperature time is increased by 30%-50%. At the same time, the problem of excessive precipitation is solved. The formula can slow down the precipitation speed by 80%, and the storage process does not require manual shaking every 24 hours and does not affect the storage.

[0006] To achieve the above object, the technical solution adopted by the present invention is: a long-acting boar semen diluent powder, which is a powdery mixture product, is mixed with the following raw materials in weight percentage:

[0007] Anhydrous glucose 18-19.5 parts,

[0008] 5-10 parts of B-glucan,

[0009] 1.5-3.5 parts of potassium chloride,

[0010] 4.1-5.0 parts of sodium ethylenediaminetetraethylene glycolate,

[0011] Cyclodextrin 2.5-5.0 parts,

[0012] Arginine 2.0-4.0 parts,

[0013] Vitamin E 0.5-1.0 parts,

[0014] Vitamin C 1.2-2 parts,

[0015] 5.6-8 parts of trihydroxyaminomethylmethane,

[0016] 2.0-4.0 parts of citric acid,

[0017] 4-5 parts of sodium bicarbonate,

[0018] 1.0-3.3 parts of sodium dehydroacetate.

[0019] The present application also provides a method for preparing a long-acting boar semen diluent powder, comprising the following steps:

[0020] S1. Weigh the raw materials according to the formula, and then continue to dry the raw materials at a drying temperature of 50°C;

[0021] S2. The trihydroxyaminomethylmethane and citric acid are retained in their original particle sizes and are packaged separately;

[0022] S3. crushing other raw materials;

[0023] S4. The crushed raw materials are added to the mixer, the mixing time is 20-30min, the speed is 10-20r / min;

[0024] S5. Pack the independently packaged trihydroxyaminomethylmethane, independently packaged citric acid and other mixed raw materials into bags.

[0025] When in use, weigh 18-19.5g of anhydrous glucose, 5-10g of B-glucan, 1.5-3.5g of potassium chloride, 4.1-5.0g of sodium ethylenediaminetetraethylene glycolate, 2.5-5.0g of cyclodextrin, 2.0-4.0g of arginine, 0.5-1.0g of vitamin E, 1.2-2g of vitamin C, 5.6-8g of trihydroxyaminomethylmethane, 2.0-4.0g of citric acid, 4-5g of sodium bicarbonate, and 1.0-3.3g of sodium dehydroacetate to make a long-acting boar semen diluent powder, then dilute and dissolve it with 1000ml of 35℃ pure water to make a long-acting boar semen diluent solution. After 30 minutes, the long-acting boar semen diluent solution and the boar semen are mixed and diluted in a ratio of 3:1 or more, and the diluted semen is stored in a constant temperature refrigerator at 4-18 degrees without turning it over.

[0026] The beneficial effects of the present invention are as follows: the present invention has four main components, namely, a nutrient agent, a buffer, a pH regulator and an antibacterial agent, as well as two components, namely, a nutrient agent regulator and an osmotic pressure regulator, which improves the activity and survival time of sperm in the pig semen diluent, and allows the pig semen diluted by the pig semen diluent to be stored for 9-20 days at a room temperature of 3-30°C. At the same time, the product solves the disadvantage that the pig semen is slow to warm up after being stored at a temperature of 4-20 degrees, and the warming up time is increased by 30%-50%. At the same time, the problem of too fast precipitation is solved. The formula can slow down the precipitation speed by 80%, and the storage process does not require manual shaking every 24 hours and does not affect the storage. DETAILED DESCRIPTION Embodiment 1:

[0027] The present invention relates to a long-acting boar semen diluting powder, which is a powdery mixture product, and the following components are added in amounts per 1L of distilled water:

[0028] Anhydrous glucose 18g, B-glucan 5g, potassium chloride 1.5g, sodium edetate 4.1g, cyclodextrin 2.5g, arginine 2.0g, vitamin E 0.5g, vitamin C 1.2g, trihydroxyaminomethylmethane 5.6g, citric acid 2.0g, sodium bicarbonate 4g, sodium dehydroacetate 1.0g.

[0029] S1. Weigh the raw materials according to the formula, and then continue to dry the raw materials at a drying temperature of 50°C;

[0030] S2. Keep the original particle size of trihydroxyaminomethylmethane and citric acid, and package them separately to prevent pH difference reaction with other materials;

[0031] S3. crushing other raw materials;

[0032] S4. The crushed raw materials were added to the mixer, the mixing time was 20min, the speed was 10r / min;

[0033] S5. Pack the independently packaged trihydroxyaminomethylmethane, independently packaged citric acid and other mixed raw materials into bags.

[0034] When in use, mix the mixed raw materials (18g anhydrous glucose, 5g B-glucan, 1.5g potassium chloride, 4.1g sodium ethylenediaminetetraethylene glycolate, 2.5g cyclodextrin, 2.0g arginine, 0.5g vitamin E, 1.2g vitamin C, 4g sodium bicarbonate, 1.0g sodium dehydroacetate) with 5.6g of independently packaged trihydroxyaminomethylmethane and 2.0g of independently packaged citric acid, and add 1000ml of 35℃ pure water to dilute and dissolve to make a long-acting boar semen dilution solution. After 30 minutes, the long-acting boar semen dilution solution and the boar semen are mixed and diluted in a ratio of 3:1. The diluted semen is stored in a constant temperature refrigerator at 17 degrees without turning it over. Embodiment 2:

[0035] A long-acting boar semen diluent powder is a powdered mixture product, and the following components are added in amounts per 1L of distilled water:

[0036] Anhydrous glucose 19.5g, B-glucan 10g, potassium chloride 3.5g, sodium ethylenediaminetetraethylene glycolate 5.0g, cyclodextrin 5.0g, arginine 4.0g, vitamin E 1.0g, vitamin C 2g, trihydroxyaminomethylmethane 8g, citric acid 4.0g, sodium bicarbonate 5g, sodium dehydroacetate 3.3g.

[0037] S1. Weigh the raw materials according to the formula, and then continue to dry the raw materials at a drying temperature of 50°C;

[0038] S2. Keep the original particle size of trihydroxyaminomethylmethane and citric acid, and package them separately to prevent pH difference reaction with other materials;

[0039] S3. crushing other raw materials;

[0040] S4. The crushed raw materials were added to the mixer, the mixing time was 20min, the speed was 10r / min;

[0041] S5. Pack the independently packaged trihydroxyaminomethylmethane, independently packaged citric acid and other mixed raw materials into bags.

[0042] When in use, the mixed raw materials (19.5g of anhydrous glucose, 10g of B-glucan, 3.5g of potassium chloride, 5.0g of sodium ethylenediaminetetraethylene glycolate, 5.0g of cyclodextrin, 4.0g of arginine, 1.0g of vitamin E, 2g of vitamin C, 5g of sodium bicarbonate, and 3.3g of sodium dehydroacetate) are mixed with 8.0g of independently packaged trihydroxyaminomethylmethane and 4.0g of independently packaged citric acid, and 1000ml of 35℃ purified water is added to dilute and dissolve to prepare a long-acting boar semen dilution solution. After 30 minutes, the long-acting boar semen dilution solution is mixed and diluted with the boar semen in a ratio of 3:1. The diluted semen is stored in a constant temperature refrigerator at 18 degrees without turning it over. Embodiment 3:

[0043] A long-acting boar semen diluent powder is a powdered mixture product, and the following components are added in amounts per 1L of distilled water:

[0044] Anhydrous glucose 19g, B-glucan 8g, potassium chloride 3.0g, sodium edetate 4.5g, cyclodextrin 3g, arginine 3.0g, vitamin E 0.8g, vitamin C 1.5g, trihydroxyaminomethylmethane 7g, citric acid 3.0g, sodium bicarbonate 4.5g, sodium dehydroacetate 3g

[0045] S1. Weigh the raw materials according to the formula, and then continue to dry the raw materials at a drying temperature of 50°C;

[0046] S2. Keep the original particle size of trihydroxyaminomethylmethane and citric acid, and package them separately to prevent pH difference reaction with other materials;

[0047] S3. crushing other raw materials;

[0048] S4. The crushed raw materials were added to the mixer, the mixing time was 20min, the speed was 10r / min;

[0049] S5. Pack the independently packaged trihydroxyaminomethylmethane, independently packaged citric acid and other mixed raw materials into bags.

[0050] When in use, mix the mixed raw materials (19g anhydrous glucose, 8g B-glucan, 3.0g potassium chloride, 4.5g sodium ethylenediaminetetraethylene glycolate, 3g cyclodextrin, 3.0g arginine, 0.8g vitamin E, 1.5g vitamin C, 4.5g sodium bicarbonate, 3g sodium dehydroacetate) with 7.0g of independently packaged trihydroxyaminomethylmethane and 3.0g of independently packaged citric acid, and add 1000ml of 35℃ pure water to dilute and dissolve to make a long-acting boar semen dilution solution. After 30 minutes, the long-acting boar semen dilution solution and the boar semen are mixed and diluted in a ratio of 3:1. The diluted semen is stored in a constant temperature refrigerator at 16 degrees without turning it over.

[0051] In the present invention, anhydrous glucose, B-glucan, cyclodextrin, arginine, vitamin E, vitamin C, potassium chloride, and sodium ethylenediaminetetraethylenedioxyacetate are used as basic nutrients to reduce the energy consumption of the sperm of the sow, so as to prolong the life of the sperm. In addition, the anhydrous glucose used in the present invention not only serves as a nutrient, but also plays a role in regulating osmotic pressure. At the same time, when the temperature is low, the sperm is easily stimulated by low temperature and suffers from low temperature shock. Therefore, in order to avoid the above situation, the present invention also uses B-glucan and cyclodextrin, which also play a role in resisting low temperature shock and accelerating temperature recovery. At the same time, sodium bicarbonate and sodium dehydroacetate are used to keep the pH of the semen basically stable. In addition, sodium dehydroacetate can play a role in preventing the growth of bacteria.

[0052] In order to test the performance data of the long-acting boar semen diluent powder of this application, the following comparative test is made

[0053] Test subjects:

[0054] Patent group: The diluted semen was prepared according to the steps of Example 1, and then quantitatively extracted every other day and tested for vitality and warming time.

[0055] Comparative group: The same batch of boar semen was diluted in the same proportion using dilution powder on the market, and stored in the same container and at the same storage temperature. Then, quantitative extraction was performed every other day and vitality and rewarming time tests were performed.

[0056] Extract and test; the comparison test table between this patent group and the comparison group on the market is as follows:

[0057] Number of days to save Patent Group vitality Recovery time Comparison group vitality Recovery time 1 Survival rate 95% Vitality 0.9 120 seconds Survival rate 93% Vitality 0.9 180 seconds 2 Survival rate 95% Vitality 0.9 120 seconds Survival rate 93% Vitality 0.85 180 seconds 3 Survival rate 95% Vitality 0.9 120 seconds Survival rate 93% Vitality 0.8 180 seconds 4 Survival rate 95% Vitality 0.9 150 seconds Survival rate 93% Vitality 0.8 220 seconds 5 Survival rate 95% Vitality 0.9 180 seconds Survival rate 93% Vitality 0.8 270 seconds 6 Survival rate 95% Vitality 0.9 200 seconds Survival rate 93% Vitality 0.8 300 seconds 7 Survival rate 95% Vitality 0.9 220 seconds Survival rate 89% Vitality 0.8 330 seconds 8 Survival rate 90% Vitality 0.9 300 seconds Survival rate 83% Vitality 0.8 400 seconds 9 Survival rate 89% Vitality 0.9 300 seconds Survival rate 75% Vitality 0.7 450 seconds 10 Survival rate 85% Vitality 0.9 360 seconds Survival rate 69% Vitality 0.7 520 seconds 11 Survival rate 83% Vitality 0.9 420 seconds Survival rate 64% Vitality 0.6 640 seconds 12 Survival rate 80% Vitality 0.9 500 seconds Survival rate 59% Vitality 0.6 700 seconds 13 Survival rate 70% Vitality 0.9 500 seconds Survival rate 40% Vitality 0.6 700 seconds 14 Survival rate 50% Vitality 0.9 500 seconds Survival rate 38% Vitality 0.6 750 seconds 15 Survival rate 30% Vitality 0.9 560 seconds Survival rate 27% Vitality 0.6 800 seconds 16 Survival rate 23% Vitality 0.9 600 seconds Survival rate 15% Vitality 0.6 800 seconds 17 Survival rate 20% Vitality 0.8 600 seconds Survival rate 9% Vitality 0.6 800 seconds 18 Survival rate 17% Vitality 0.8 600 seconds Survival rate 2% Vitality 0.6 800 seconds 19 Survival rate 12% Vitality 0.8 600 seconds Survival rate 1% Vitality 0.6 800 seconds 20 Survival rate 8% Vitality 0.8 600 seconds Survival rate 0% Vitality 0.6 800 seconds

[0058] From the above test data, we can see that:

[0059] The warming time of the patent group in this application is 25%-30% faster than that of the control group. Moreover, from the table data, it can be clearly seen that the use of the long-acting boar semen diluent improves the activity and survival time of the sperm in the boar semen diluent, allowing the boar semen diluted with the boar semen diluent to be stored at a room temperature of 3~30℃ for 9-20 days.

[0060] The above implementation modes are merely descriptions of the preferred implementation modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary engineering and technical personnel in the field shall fall within the protection scope determined by the claims of the present invention.

Claims

1. A long-acting boar semen diluent powder is a powdery mixture product, characterized in that: The composition is prepared by mixing the following raw materials in percentage by weight: 34.8-50 parts of nutrient agent, 5.6-8 parts of buffer agent, 6-9 parts of pH regulator, and 1-3.3 parts of antibacterial agent; wherein the nutrient agent is prepared by mixing the following raw materials in percentage by weight: Anhydrous glucose 18-19.5 parts, 5-10 parts of B-glucan, 1.5-3.5 parts of potassium chloride, 4.1-5.0 parts of sodium ethylenediaminetetraethylene glycolate, Cyclodextrin 2.5-5.0 parts, Arginine 2.0-4.0 parts, Vitamin E 0.5-1.0 parts, Vitamin C 1.2-2 parts; The buffer is composed of the following raw materials in parts by mass: 5.6-8 parts of trihydroxyaminomethylmethane; the pH adjuster is a mixture of the following raw materials in parts by mass: 2.0-4.0 parts of citric acid and 4-5 parts of sodium bicarbonate; and the antibacterial agent is composed of the following raw materials in parts by mass: 1.0-3.3 parts of sodium dehydroacetate.

2. The method for preparing a long-acting boar semen diluent according to claim 1, characterized in that: The steps include: S1. Weigh the raw materials according to the formula, and then continue to dry the raw materials at a drying temperature of 50°C; S2. The trihydroxyaminomethylmethane and citric acid are retained in their original particle sizes and are packaged separately; S3. crushing other raw materials; S4. The crushed raw materials are added to the mixer, the mixing time is 20-30min, the speed is 10-20r / min; S5. Pack the independently packaged trihydroxyaminomethylmethane, independently packaged citric acid and other mixed raw materials into bags.