A skin care cosmetic composition containing phytoestrogen, cosmetics and preparation method thereof

Abstract

The present invention provides a skin care cosmetic composition containing phytoestrogens, cosmetics and preparation methods thereof. The skin care cosmetic composition containing phytoestrogens includes Cimicifuga racemosa root extract, soybean seed extract, Pueraria root extract, Ginkgo leaf extract, Belamcanda root extract and Rosa roxburghii fruit extract. It has dual anti-aging and light protection effects. The gel mask of the present invention includes a film-like gel carrier, which is prepared by mixed reaction of dialdehyde-modified chitosan and polyethyleneimine (PEI). The adsorption capacity of the essence is large, more essence can be loaded, and the gel has good antibacterial properties, avoiding the use of preservatives such as p-hydroxyacetophenone in cosmetics, and avoiding the harm of preservatives to the human body. The gel prepared by cross-linking chitosan and polyethyleneimine is used for the gel mask, which has a good sustained-release effect, ensures the long-term release of the essence, improves the efficacy of the cosmetics, and avoids the waste of the essence.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and in particular to a skin care cosmetic composition containing phytoestrogen, cosmetics and a preparation method thereof. Background Art

[0002] In order to maintain the normal physiological functions of the skin and delay skin aging, more and more people have begun to use skin care cosmetics to care for their skin, and in recent years plant extracts have gradually replaced synthetic chemicals in cosmetics.

[0003] At present, the existing essence liquid combined with plant extracts is loaded on fiber cloth to make a mask for use, but the loading rate of the essence liquid is low, and the release effect is extremely fast, and the skin cannot absorb it well. Even if there are gel masks, the essence liquid adsorption capacity is low, and its release effect is poor. A large amount of essence liquid will be released in the early stage of use, causing the essence liquid to overflow on the face. In addition, gel masks are prone to breeding bacteria, and have high requirements for packaging. Summary of the invention

[0004] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a skin care cosmetic composition containing phytoestrogen, cosmetics and a preparation method thereof.

[0005] To achieve the above-mentioned purpose, the technical scheme adopted by the present invention is: a skin care cosmetic composition containing phytoestrogen, which comprises 5-10 parts by weight of Cimicifuga racemosa root extract, 1-3 parts by weight of Glycine soja seed extract, 1-3 parts by weight of Pueraria lobata root extract, 1-3 parts by weight of Ginkgo biloba leaf extract, 1-3 parts by weight of Belamcanda chinensis root extract, and 1-3 parts by weight of Rosa roxburghii fruit extract.

[0006] The above-mentioned phytoestrogen-containing skin care cosmetic composition has a high content of Cimicifuga racemosa extract, and when combined with other components, it has dual anti-aging and photoprotection effects. When combined with other plant extracts that are rich in flavonoids, it can help with skin hormone deficiency, rejuvenate the skin, whiten the skin, and maintain the skin's youthful state.

[0007] Preferably, the skin care cosmetic composition comprises 5-10 parts by weight of Cimicifuga racemosa root extract, 1-3 parts by weight of Glycine soja seed extract, 1-3 parts by weight of Pueraria lobata extract, 1-3 parts by weight of Ginkgo biloba leaf extract, 1-3 parts by weight of Belamcanda chinensis root extract, 1-3 parts by weight of Rosa roxburghii fruit extract and 0.01-0.5% of tetrahydropiperine.

[0008] The above-mentioned phytoestrogen-containing skin care cosmetic composition contains tetrahydropiperine, which is beneficial to promoting the skin to absorb the functional components in the cosmetics.

[0009] The present invention also provides a gel mask, which comprises a film-like gel carrier and an essence;

[0010] The essence comprises the following components in weight percentage: 5%-10% of Cimicifuga racemosa root extract, 1%-5% of Dioscorea zingiberensis root extract, 1%-3% of Glycine max seed extract, 1%-3% of Pueraria lobata root extract, 1%-3% of Ginkgo biloba leaf extract, 1%-3% of Belamcanda chinensis root extract, 1%-3% of Rosa roxburghii fruit extract, 0.01-0.5% of tetrahydropiperine, 2%-4% of isononyl isononanoate, 0.5%-2% of hydrogenated lecithin and the balance of water;

[0011] The gel carrier is prepared by mixing and reacting dialdehyde-modified chitosan and polyethyleneimine (PEI), wherein the average molecular weight of the polyethyleneimine is 2100 to 80000, and the structural formula of the dialdehyde-modified chitosan is as shown in Formula I:

[0012]

[0013] The dialdehyde-modified chitosan is prepared by oxidation of chitosan with sodium periodate. As shown in Formula II,

[0014]

[0015] The reaction steps of the double-aldehyde chitosan are as follows: chitosan is mixed with a sodium periodate aqueous solution, protected by nitrogen, and protected from light in a dark room, and reacted at a constant temperature of 25 to 35°C for 2 to 3 hours with shaking, and the ratio of chitosan to sodium periodate is 1g chitosan to 0.08 to 0.15 mol sodium periodate. The reaction is terminated by adding ethylene glycol solution, and after standing, the unreacted chitosan is filtered; an appropriate amount of acetone is added to the filtrate, and after precipitation for 2 hours, the filtrate is filtered by suction, and then washed with ethanol and dehydrated, and finally dried to obtain the double-aldehyde chitosan.

[0016] The dialdehyde-modified chitosan chain contains a large number of aldehyde groups and is active in nature. Polyethyleneimine contains amino groups, which can react with dialdehyde-modified chitosan to produce Schiff base reaction. The amino groups on polyethyleneimine and the aldehyde groups on dialdehyde-modified chitosan react quickly to form a cross-linked network. The polyethyleneimine aqueous solution reacts rapidly after mixing with the dialdehyde-modified chitosan aqueous solution, and the viscosity of the solution rises rapidly, forming a gel within 10 to 15 seconds. To prepare a gel mask carrier, the polyethyleneimine solution and the dialdehyde-modified chitosan solution need to be mixed, mixed and evenly distributed into a film within 10 seconds to form a gel film. The gel prepared by cross-linking dialdehyde-modified chitosan and polyethyleneimine is used for gel masks. It has a large adsorption capacity for essences and can load more essences. The gel has good antibacterial properties, avoiding the use of preservatives such as parahydroxyacetophenone in cosmetics and avoiding the harm of preservatives to the human body. The gel prepared by cross-linking chitosan and polyethyleneimine is used in gel masks, which has a good sustained-release effect, ensures the long-term release of essence, improves the efficacy of cosmetics, and avoids the waste of essence. Isononyl isononanoate and hydrogenated lecithin are added to the essence. Isononyl isononanoate and hydrogenated lecithin cooperate with each other to improve the retention of essence in the gel, further improve the sustained-release effect, and avoid the overflow of essence in the early stage of mask use.

[0017] Preferably, the preparation method of the gel carrier is to homogenize and mix a polyethyleneimine aqueous solution with a mass concentration of 5%-10% and a dialdehyde chitosan aqueous solution with a mass concentration of 5-10% within 10-15 seconds, and evenly distribute them into a film of 0.1-0.3 mm.

[0018] Preferably, the ratio of the essence to the film-like gel carrier is 30 to 80 grams of essence / gram of the film-like gel carrier.

[0019] Preferably, the mass ratio of the polyethyleneimine to the dialdehyde-modified chitosan is (0.5-3):1.

[0020] Preferably, the average molecular weight of the polyethyleneimine is 5000-10000, and the mass ratio of the polyethyleneimine to the dialdehyde-modified chitosan is (1.5-2):1.

[0021] When the average molecular weight of polyethyleneimine is 5000-10000, and the mass ratio of polyethyleneimine to dialdehyde-modified chitosan is (1.5-2):1, the gel carrier can swell and absorb the essence faster, and swell a larger amount of essence within 20-40 minutes, which is conducive to the rapid preparation of the gel mask, saving the time of assembly line production and reducing costs.

[0022] Preferably, the essence comprises the following components in weight percentage: 5%-10% of Cimicifuga racemosa root extract, 1%-5% of Dioscorea zingiberensis root extract, 1%-3% of Glycine max seed extract, 1%-3% of Pueraria lobata root extract, 1%-3% of Ginkgo biloba leaf extract, 1%-3% of Belamcanda chinensis root extract, 1%-3% of Rosa roxburghii fruit extract, 0.01-0.5% of tetrahydropiperine, 2.5%-3% of isononyl isononanoate, 1%-1.5% of hydrogenated lecithin and the balance of water.

[0023] Isononyl isononanoate and hydrogenated lecithin are added to the essence. Isononyl isononanoate and hydrogenated lecithin cooperate with each other to improve the retention of the essence in the gel. When the weight content of isononyl isononanoate in the essence is 2.5%-3% and the weight content of hydrogenated lecithin is 1%-1.5%, the sustained-release effect is further enhanced, and the long-lasting effect of the gel mask is improved.

[0024] Preferably, the essence comprises the following components in weight percentage: 6%-8% of Cimicifuga racemosa root extract, 2%-4% of Dioscorea zingiberensis root extract, 2%-2.5% of Glycine max seed extract, 1.5%-2% of Pueraria lobata root extract, 2%-3% of Ginkgo biloba leaf extract, 1%-3% of Belamcanda chinensis root extract, 1%-3% of Rosa roxburghii fruit extract, 0.01-0.5% of tetrahydropiperine, 2.5%-3% of isononyl isononanoate, 1%-1.5% of hydrogenated lecithin and the balance of water.

[0025] Preferably, the essence further comprises the following components in weight percentage: 3%-8% butylene glycol, 3%-8% glycerol, 3%-8% caprylic / capric triglyceride, 1%-3% jojoba seed oil, 1%-3% cetearyl alcohol, 1%-3% cetearyl glucoside, 0.1%-0.3% carbomer, and 0.05%-0.2% xanthan gum.

[0026] The present invention also provides a method for preparing any of the above-mentioned gel facial masks, comprising homogenizing the components included in the essence to obtain the essence, and swelling the film-like gel carrier in the essence for 20 to 40 minutes.

[0027] The beneficial effects of the present invention are as follows: the present invention provides a skin care cosmetic composition containing phytoestrogens, cosmetics and preparation methods thereof. The skin care cosmetic composition containing phytoestrogens of the present invention has a high content of Cimicifuga racemosa extract, and when matched with other components, has dual anti-aging and light protection effects. When matched with other plant extracts, it contains rich flavonoid components, which can help the skin hormone deficiency, skin rejuvenation, whitening and freckle removal, and maintain the youthful state of the skin. The gel mask of the present invention made of dialdehyde has a large adsorption capacity for essence, can load more essence, and the gel has good antibacterial properties, avoids the use of preservatives such as parahydroxyacetophenone in cosmetics, and avoids the harm of preservatives to the human body. The gel prepared by cross-linking chitosan and polyethyleneimine is used for the gel mask, has a good sustained release effect, ensures the long-term release of the essence, improves the efficacy of cosmetics, and avoids the waste of the essence. Isononyl isononanoate and hydrogenated lecithin are added to the essence, and isononyl isononanoate and hydrogenated lecithin cooperate with each other to improve the retention of the essence in the gel, further improve the sustained release effect, and avoid the overflow of the essence in the initial use of the gel mask. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a diagram showing the long-acting sustained-release effect of the gel mask of the present invention. DETAILED DESCRIPTION

[0029] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0030] Example 1

[0031] As a gel mask in an embodiment of the present invention, the gel mask comprises a film-like gel carrier and an essence;

[0032] The essence comprises the following components in weight percentage: 6% of Cimicifuga racemosa root extract, 2% of Dioscorea zingiberensis root extract, 2.5% of Glycine max seed extract, 1.5% of Pueraria lobata root extract, 2% of Ginkgo biloba leaf extract, 2% of Belamcanda chinensis root extract, 2% of Rosa roxburghii fruit extract, 0.2% of Tetrahydropiperine, 2.5% of Isononanoate, 1% of Hydrogenated Lecithin, 5% of Butylene Glycol, 5% of Glycerin, 5% of Caprylic / Capric Triglyceride, 2% of Jojoba Seed Oil, 2% of Cetearyl Alcohol, 2% of Cetearyl Glucoside, 0.2% of Carbomer, 0.1% of Xanthan Gum and the balance of water;

[0033] The gel carrier is prepared by mixing and reacting dialdehyde-modified chitosan and polyethyleneimine (PEI), wherein the average molecular weight of the polyethyleneimine is 5000 (average Mn-5000 (calculated by GPC)), and the structural formula of the dialdehyde-modified chitosan is as shown in Formula I:

[0034]

[0035] The mass ratio of polyethyleneimine to dialdehyde-modified chitosan is 1.5:1, and the essence and the film-like gel carrier are absorbed and swelled for 30 minutes at a mass ratio of 80:1.

[0036] Example 2

[0037] As a gel mask of an embodiment of the present invention, the gel mask comprises a film-like gel carrier and an essence. The only difference between this embodiment and embodiment 1 is that the content components of the essence are different:

[0038] The essence comprises the following components in weight percentage: 5% of Cimicifuga racemosa root extract, 2% of Dioscorea zingiberensis root extract, 2.5% of Glycine max seed extract, 1.5% of Pueraria lobata root extract, 2% of Ginkgo biloba leaf extract, 2% of Belamcanda chinensis root extract, 2% of Rosa roxburghii fruit extract, 0.2% of tetrahydropiperine, 2.5% of isononyl isononanoate, 1% of hydrogenated lecithin, 5% of butylene glycol, 5% of glycerol, 5% of caprylic / capric triglyceride, 2% of jojoba seed oil, 2% of cetearyl alcohol, 2% of cetearyl glucoside, 0.2% of carbomer, 0.1% of xanthan gum and the remainder of water.

[0039] Example 3

[0040] As a gel mask of an embodiment of the present invention, the gel mask comprises a film-like gel carrier and an essence. The only difference between this embodiment and embodiment 1 is that the content components of the essence are different:

[0041] The essence comprises the following components in weight percentage: 8% of Cimicifuga racemosa root extract, 2% of Dioscorea zingiberensis root extract, 2.5% of Glycine max seed extract, 1.5% of Pueraria lobata root extract, 2% of Ginkgo biloba leaf extract, 2% of Belamcanda chinensis root extract, 2% of Rosa roxburghii fruit extract, 0.2% of tetrahydropiperine, 2.5% of isononyl isononanoate, 1% of hydrogenated lecithin, 5% of butylene glycol, 5% of glycerol, 5% of caprylic / capric triglyceride, 2% of jojoba seed oil, 2% of cetearyl alcohol, 2% of cetearyl glucoside, 0.2% of carbomer, 0.1% of xanthan gum and the remainder of water.

[0042] Example 4

[0043] As a gel mask of an embodiment of the present invention, the gel mask comprises a film-like gel carrier and an essence. The only difference between this embodiment and embodiment 1 is that the content components of the essence are different:

[0044] The essence comprises the following components in weight percentage: 10% of Cimicifuga racemosa root extract, 2% of Dioscorea zingiberensis root extract, 2.5% of Glycine max seed extract, 1.5% of Pueraria lobata root extract, 2% of Ginkgo biloba leaf extract, 2% of Belamcanda chinensis root extract, 2% of Rosa roxburghii fruit extract, 0.2% of tetrahydropiperine, 2.5% of isononyl isononanoate, 1% of hydrogenated lecithin, 5% of butylene glycol, 5% of glycerol, 5% of caprylic / capric triglyceride, 2% of jojoba seed oil, 2% of cetearyl alcohol, 2% of cetearyl glucoside, 0.2% of carbomer, 0.1% of xanthan gum and the balance of water.

[0045] Example 5

[0046] As a gel mask of an embodiment of the present invention, the gel mask comprises a film-like gel carrier and an essence. The only difference between this embodiment and embodiment 1 is that the content components of the essence are different:

[0047] The essence comprises the following components in weight percentage: 6% of Cimicifuga racemosa root extract, 4% of Dioscorea zingiberensis root extract, 2% of Glycine max seed extract, 1.5% of Pueraria lobata root extract, 2% of Ginkgo biloba leaf extract, 2% of Belamcanda chinensis root extract, 2% of Rosa roxburghii fruit extract, 0.2% of tetrahydropiperine, 2.5% of isononyl isononanoate, 1% of hydrogenated lecithin, 5% of butylene glycol, 5% of glycerol, 5% of caprylic / capric triglyceride, 2% of jojoba seed oil, 2% of cetearyl alcohol, 2% of cetearyl glucoside, 0.2% of carbomer, 0.1% of xanthan gum and the remainder of water.

[0048] Example 6

[0049] As a gel mask of an embodiment of the present invention, the gel mask comprises a film-like gel carrier and an essence. The only difference between this embodiment and embodiment 1 is that the content components of the essence are different:

[0050] The essence comprises the following components in weight percentage: 6% of Cimicifuga racemosa root extract, 4% of Dioscorea zingiberensis root extract, 2% of Glycine max seed extract, 1% of Pueraria lobata root extract, 3% of Ginkgo biloba leaf extract, 2% of Belamcanda chinensis root extract, 2% of Rosa roxburghii fruit extract, 0.2% of tetrahydropiperine, 2.5% of isononyl isononanoate, 1% of hydrogenated lecithin, 5% of butylene glycol, 5% of glycerol, 5% of caprylic / capric triglyceride, 2% of jojoba seed oil, 2% of cetearyl alcohol, 2% of cetearyl glucoside, 0.2% of carbomer, 0.1% of xanthan gum and the remainder of water.

[0051] Example 7

[0052] As a gel mask of an embodiment of the present invention, the gel mask comprises a film-like gel carrier and an essence; the only difference between this embodiment and embodiment 1 is that the film-like gel carrier is different;

[0053] The average molecular weight of the polyethyleneimine is 2100 (average Mn ˜2100 (calculated by GPC)).

[0054] Example 8

[0055] As a gel mask of an embodiment of the present invention, the gel mask comprises a film-like gel carrier and an essence; the only difference between this embodiment and embodiment 1 is that the film-like gel carrier is different;

[0056] The average molecular weight of the polyethyleneimine is 10,000 (average Mn -10,000 (calculated by GPC)).

[0057] Example 9

[0058] As a gel mask of an embodiment of the present invention, the gel mask comprises a film-like gel carrier and an essence; the only difference between this embodiment and embodiment 1 is that the film-like gel carrier is different;

[0059] The average molecular weight of the polyethyleneimine is 60,000 (average Mn - 10,000 (calculated by GPC)).

[0060] Example 10

[0061] As a gel mask of an embodiment of the present invention, the gel mask includes a film-like gel carrier and an essence; the only difference between this embodiment and Example 1 is that the film-like gel carrier is different; the mass ratio of polyethyleneimine to dialdehyde-modified chitosan is 0.5:1.

[0062] Embodiment 11

[0063] As a gel mask of an embodiment of the present invention, the gel mask includes a film-like gel carrier and an essence; the only difference between this embodiment and Example 1 is that the film-like gel carrier is different; the mass ratio of polyethyleneimine to dialdehyde-modified chitosan is 1:1.

[0064] Example 12

[0065] As a gel mask of an embodiment of the present invention, the gel mask includes a film-like gel carrier and an essence; the only difference between this embodiment and Example 1 is that the film-like gel carrier is different; the mass ratio of polyethyleneimine to dialdehyde-modified chitosan is 2:1.

[0066] Example 13

[0067] As a gel mask of an embodiment of the present invention, the gel mask includes a film-like gel carrier and an essence; the only difference between this embodiment and Example 1 is that the film-like gel carrier is different; the mass ratio of polyethyleneimine to dialdehyde-modified chitosan is 3:1.

[0068] Comparative Example 1

[0069] As a comparative example of the present invention, a gel mask comprises a film-like gel carrier and an essence; the only difference between this comparative example and Example 1 is that the film-like gel carrier is different;

[0070] The average molecular weight of the polyethyleneimine is 1200 (average Mn ˜1200 (calculated by GPC)).

[0071] Comparative Example 2

[0072] As a comparative example of the present invention, a gel mask comprises a film-like gel carrier and an essence. The only difference between this comparative example and Example 1 is that the content components of the essence are different:

[0073] The essence does not contain isononyl isononanoate, and the corresponding weight ratio is supplemented with water.

[0074] Comparative Example 3

[0075] As a comparative example of the present invention, a gel mask comprises a film-like gel carrier and an essence. The only difference between this comparative example and Example 1 is that the content components of the essence are different:

[0076] The essence does not contain hydrogenated lecithin and the corresponding weight ratio is supplemented with water.

[0077] Comparative Example 4

[0078] As a comparative example of the present invention, a gel mask comprises a film-like gel carrier and an essence. The only difference between this comparative example and Example 1 is that the film-like gel carrier is different.

[0079] The gel carrier of this comparative example is: chitosan / gelatin / polyvinyl alcohol gel film; the preparation method comprises the following steps:

[0080] (1) Dissolve chitosan and citric acid in an appropriate amount of distilled water at a mass ratio of 1:2, stir magnetically until the chitosan is completely dissolved, let stand to degas, and set aside; the mass concentration of chitosan is 6%;

[0081] (2) Prepare a 5% gelatin solution;

[0082] (3) Slowly add the gelatin solution to the chitosan solution, continue heating and stirring until the solution becomes a transparent liquid, keep it at 25 degrees for a certain period of time, and remove bubbles. Pour the mixed blended solution into the mask mold, make it evenly distributed, and then freeze-thaw for 5 times;

[0083] (4) preparing a 10% polyvinyl alcohol solution;

[0084] (5) Add the polyvinyl alcohol solution to the mixed solution obtained in step (3) in a certain proportion and blend them, continue to heat to 25 degrees, keep the stirring rate unchanged, until the solution becomes a transparent liquid, keep warm for a certain period of time, and remove bubbles. Pour the mixed solution into a mask mold, perform a freeze-thaw cycle, and obtain a gel film.

[0085] Freeze-thaw process flow: drop from 25℃ to -20℃, freeze for 1 hour, then warm up to room temperature, keep warm for 1 hour, and repeat this cycle.

[0086] Comparative Example 5

[0087] As a comparative example of the present invention, a gel mask comprises a film-like gel carrier and an essence. The only difference between this comparative example and Example 1 is that the content components of the essence are different: the essence does not contain Cimicifuga racemosa extract, and the corresponding content is supplemented with water.

[0088] Experimental example

[0089] 1. Experimental materials

[0090] 1. Plant extracts

[0091] Cimicifuga racemosa root extract was purchased from Guangzhou Baihaobo Co., Ltd., Dioscorea villosa root extract was purchased from SBC Chemical (Shanghai) Co., Ltd.; Glycine max seed extract was purchased from Cosfa International Trade (Shanghai) Co., Ltd., Pueraria lobata root extract was purchased from Guangzhou Xufan Trading Co., Ltd., Ginkgo biloba leaf extract was purchased from Guangzhou Junming Trading Co., Ltd., Belamcanda chinensis root extract was purchased from Guangzhou Tongyi Chemical Co., Ltd., and Rosa roxburghii fruit extract was purchased from Guangzhou Ruiran Biotechnology Co., Ltd.

[0092] 2. Polyethyleneimine was purchased from Aladdin.

[0093] 3. Preparation of dialdehyde-modified chitosan

[0094] Add 30g chitosan and 1500mL 0.3mol / L sodium periodate solution, mix, protect with nitrogen, dark room from light, and react at 30℃ constant temperature oscillation for 2h. Add 200mL 0.1mol / L ethylene glycol solution to terminate the reaction, let stand for 0.5h, filter out the unreacted chitosan; add 300mL acetone to the filtrate, precipitate for 2h, filter, wash with ethanol and dehydrate, and finally dry in an oven at 70℃ to obtain dialdehyde chitosan. Set aside. The preparation can be repeated multiple times.

[0095] 2. Sample preparation

[0096] 1. Preparation of essence: Mix the essence according to the composition content of the embodiment and comparative example. Mark each embodiment and comparative example, and set the essence aside.

[0097] 2. Preparation of gel carrier: The preparation method of gel carrier is to homogenize and mix a 6% polyethyleneimine aqueous solution and a 6% dialdehyde chitosan aqueous solution within 10-15 seconds, and evenly distribute them into a 0.15 mm film. In each embodiment, the volume ratio of the solution is selected according to the molecular weight of polyethyleneimine and the mass ratio of polyethyleneimine to chitosan.

[0098] 3. To facilitate the sample, the gel carrier was cut into 2cm×3cm specifications for subsequent tests.

[0099] 3. Antioxidant samples.

[0100] 1. Determination of the ability to scavenge hydroxyl free radicals

[0101] Accurately pipette 20 μL of the essence of Examples 1 to 6 and Comparative Example 5 into a 96-well plate, add 60 μL of 0.1 mmol / L luminol reagent (prepared with 0.1 mol / L NaHCO3-Na2CO3 buffer solution with a pH of 10.5), and then add 10 μL of 0.3 mmol / L Fe 2+ -EDTA solution, 10 μL of 0.3% hydrogen peroxide solution, shake for 2 seconds to mix, incubate for 20 seconds, and measure the total luminescence intensity. The luminescence intensity without hydrogen peroxide solution is used as the background, and water is set as a blank control.

[0102] Hydroxyl radical scavenging rate (%) = ((c blank - c background) - (c sample - c background)) / (c blank - c background) × 100%.

[0103] c blank is the luminescence intensity of deionized water, c background is the luminescence intensity without adding hydrogen peroxide solution, and c sample is the luminescence intensity of the sample to be tested.

[0104] Table 1 Hydroxyl free radical scavenging rate of essence (%)

[0105] Essence sample Hydroxyl free radical scavenging rate (%) Example 1 80.5 Example 2 71.7 Example 3 78.4 Example 4 73.1 Example 5 78.4 Example 6 76.3 Comparative Example 5 43.8

[0106] From the above, it can be seen that the Cimicifuga racemosa extract combined with other components has dual anti-aging and photoprotection effects.

[0107] IV. Gel mask liquid retention test and antibacterial performance test

[0108] 1. Gel mask liquid retention test,

[0109] Cut the film-like gel carriers of Example 1, Examples 7-13, Comparative Example 1, and Comparative Example 4 into a size of 2cm×3cm, weigh them, and record them as m1. Take the essences of Example 1, Examples 7-13, Comparative Examples 1-Comparative Example 4 at a mass ratio of 80:1 with the film-like gel carrier; immerse the film-like gel carrier in the essence to swell for 30 minutes to prepare a gel mask sample, and quickly wipe off the liquid on the surface of the gel carrier with absorbent paper, which is recorded as m2.

[0110] Membrane gel carrier liquid retention w = (m2-m1) / m1×100%

[0111] 2. Antibacterial performance test

[0112] The sample used in this experiment is a film-like gel carrier.

[0113] The antibacterial ability of the film-like gel carrier was analyzed by the inhibition zone method, using Saccharomyces cerevisiae and Penicillium as sample bacteria as specified in the "Cosmetic Hygiene Standards".

[0114] Dilute the sample bacteria to 10 with sterile meat extract peptone liquid medium (hereinafter referred to as LB liquid medium). 4 CFU / ml, 1ml was taken and coated on LB solid medium, and the gel carrier was placed on the LB solid medium coated with bacteria, and the bacterial growth was observed after culturing at 37℃ for 24h. The culture method of fungi was the same as that of bacteria. The culture medium was replaced with yeast extract peptone glucose medium (hereinafter referred to as YPD medium), and the fungal coating concentration was 10 3 CFU / ml, the culture temperature was 25℃.

[0115] Place a 0.3 ml diameter gel carrier in a 24-well plate, add culture medium, and add 50 μL of 10 4 The target bacterial strain liquid of 0.05 CFU / ml was recorded as the OD600 experimental group, and a positive control without adding the gel carrier and only adding the target bacteria was set up, recorded as the OD600 positive control group, and a negative control without adding the target bacteria and only adding 3ml of the gel membrane carrier was set up, recorded as the OD600 negative control group, and cultured for 24 hours. LB liquid medium was used for the dilution and culture of bacteria, and YPD medium was used for the dilution and culture of fungi. The inhibition rate was determined by the OD600 method, and the inhibition rate X of the single-hole target bacteria was calculated as follows:

[0116] X = (OD600 experimental group - OD600 negative control group) / (OD600 positive control group - OD600 blank group) × 100%

[0117] Table 2 Liquid retention rate of film gel carrier

[0118]

[0119] From the above results, it can be seen that the gel prepared by cross-linking dialdehyde-modified chitosan and polyethyleneimine is used for gel mask, has a large adsorption capacity for essence, can load more essence, and the gel has good antibacterial properties, avoiding the use of preservatives such as p-hydroxyacetophenone in cosmetics and avoiding the harm of preservatives to the human body.

[0120] From the above results, it can be seen that when the average molecular weight of polyethyleneimine is 5000-10000, and the mass ratio of polyethyleneimine to dialdehyde-modified chitosan is (1.5-2):1, the gel carrier can swell and absorb the essence faster, and swell more essence within 20-40 minutes, which is conducive to the rapid preparation of gel masks, saving production line time and reducing costs.

[0121] 5. Gel mask sustained release effect test

[0122] The sample in this experiment is a gel mask swollen with essence. The preparation method is the same as that of experiment 4.

[0123] 24 female volunteers aged 25 to 30 were selected for on-site testing. No cosmetics were allowed to be applied to the test area on the morning of the test. The volunteers were divided into 8 groups, with three people in each group. Under the guidance of the staff, the volunteers used clean water to clean the test area (right cheek).

[0124] The skin moisture of the experimental area was tested using Comeometer CM825, and the average value was taken after three tests.

[0125] Before using the gel mask, the skin moisture of the test area of ​​8 groups of volunteers was tested, recorded as T0, and the average value of the same group area. The 8 groups of volunteers used Example 1, Example 8, Example 9, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, and the control sample, respectively, with a test mask of 2cm×3cm, and the test mask was applied to the right side of the cheek. The preparation of the essence of the control sample was exactly the same as that of Example 1, and the essence carrier was a fiber cloth.

[0126] After using the sample for 5 minutes, 10 minutes, 30 minutes, 1 hour, 2 hours, 4 hours, and 6 hours, remove the mask and use Comeometer CM825 to test the skin moisture of the experimental area. After the test, put the original mask back and continue to record the time. The skin moisture test is recorded as T1, T2, T3, T4, T5, T6, and T7 in sequence, and the average value is taken for the same group of volunteers.

[0127] The relative change rate of skin moisture is t1 = (T1-T0) / T0×100%. At different times, T1, T2, T3, T4, T5, T6, T7 replace T1 in the formula.

[0128] Table 3 Long-lasting sustained-release moisturizing effect of gel mask

[0129]

[0130] The long-term sustained release effect of the gel mask on the effective components was considered by testing the effect of long-term mask application on skin moisture. Figure 1 It can be clearly found that the gel mask of Example, Example 8, and Example 9 can increase the water content of the skin for a long time during use for up to 6 hours, indicating that the gel prepared by cross-linking chitosan and polyethyleneimine is used for gel mask, which has a good sustained release effect, can maintain the continuous release of effective ingredients for up to 6 hours, and ensure the long-term release of the essence. The release time of the effective ingredients is up to 6 hours or more, which improves the efficacy of cosmetics, avoids the waste of essence, and avoids the overflow of the essence in the early stage of the mask. When used at night, it can be released for a long time, which improves the efficacy of the mask. By comparing Comparative Example 2 and Comparative Example 3, the gel mask of Comparative Example 2 and Comparative Example 3 has a significant decrease in the improvement of the skin moisture content after 4 hours, indicating that isononyl isononanoate and hydrogenated lecithin are added to the essence, and isononyl isononanoate and hydrogenated lecithin cooperate with each other to improve the retention of the essence in the gel, and further improve the sustained release effect. Long-term release of functional components. The gel mask of comparative example 4 has a significant decrease in the improvement of skin moisture content after 1 hour, indicating that the release of the functional components of the gel mask of comparative example 4 decreases rapidly after 1 hour of use. The traditional fiber cloth mask as a control sample can only maintain the effect of releasing the functional components within 30 minutes, which also indicates that the functional components overflow and are wasted after release and cannot be absorbed by the skin for a long time.

[0131] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A gel mask, characterized in that: The gel mask comprises a film-like gel carrier and an essence; The essence comprises the following components in weight percentage: 5%-10% of Cimicifuga racemosa root extract, 1%-5% of Dioscorea zingiberensis root extract, 1%-3% of Glycine max seed extract, 1%-3% of Pueraria lobata root extract, 1%-3% of Ginkgo biloba leaf extract, 1%-3% of Belamcanda chinensis root extract, 1%-3% of Rosa roxburghii fruit extract, 0.01-0.5% of tetrahydropiperine, 2%-4% of isononyl isononanoate, 0.5%-2% of hydrogenated lecithin and the balance of water; The gel carrier is prepared by mixing and reacting dialdehyde chitosan and polyethylene imine, wherein the mass ratio of the polyethylene imine to the dialdehyde chitosan is (1.5-2):1, the average molecular weight of the polyethylene imine is 2100-10000, and the structural formula of the dialdehyde chitosan is as shown in Formula I.

2. The gel mask according to claim 1, characterized in that: The preparation method of the gel carrier is to homogenize and mix a polyethyleneimine aqueous solution with a mass concentration of 5%-10% and a dialdehyde chitosan aqueous solution with a mass concentration of 5-10% within 10-15 seconds, and evenly distribute them into a film of 0.1-0.3mm.

3. The gel mask according to claim 1, characterized in that: The preparation method of the gel mask comprises homogenizing the components included in the essence to obtain the essence, swelling the film-like gel carrier in the essence for 20 to 40 minutes, and the ratio of the essence to the film-like gel carrier is 30 to 80 g essence / g film-like gel carrier.

4. The gel mask according to claim 1, characterized in that: The mass ratio of the polyethyleneimine to the dialdehyde-modified chitosan is (0.5-3):

1.

5. The gel mask according to claim 1, characterized in that: The average molecular weight of the polyethyleneimine is 5000-10000, and the mass ratio of the polyethyleneimine to the dialdehyde-modified chitosan is (1.5-2):

1.

6. The gel mask according to claim 1, characterized in that: The essence comprises the following components in percentage by weight: 5%-10% of Cimicifuga racemosa root extract, 1%-5% of Dioscorea zingiberensis root extract, 1%-3% of Glycine max seed extract, 1%-3% of Pueraria lobata root extract, 1%-3% of Ginkgo biloba leaf extract, 1%-3% of Belamcanda chinensis root extract, 1%-3% of Rosa roxburghii fruit extract, 0.01-0.5% of tetrahydropiperine, 2.5%-3% of isononyl isononanoate, 1%-1.5% of hydrogenated lecithin and the balance of water.

7. The gel mask according to claim 1, characterized in that: The essence comprises the following components in percentage by weight: 6%-8% of Cimicifuga racemosa root extract, 2%-4% of Dioscorea zingiberensis root extract, 2%-2.5% of Glycine max seed extract, 1.5%-2% of Pueraria lobata root extract, 2%-3% of Ginkgo biloba leaf extract, 1%-3% of Belamcanda chinensis root extract, 1%-3% of Rosa roxburghii fruit extract, 0.01-0.5% of tetrahydropiperine, 2.5%-3% of isononyl isononanoate, 1%-1.5% of hydrogenated lecithin and the balance of water.

8. A gel mask, characterized in that: The gel mask comprises a film-like gel carrier and an essence. The gel carrier is prepared by mixing and reacting dialdehyde chitosan and polyethylene imine, wherein the mass ratio of the polyethylene imine to the dialdehyde chitosan is (1.5-2):1, the average molecular weight of the polyethylene imine is 2100-10000, and the structural formula of the dialdehyde chitosan is as shown in Formula I. The essence comprises the following components in weight percentage: 5%-10% of Cimicifuga racemosa root extract, 1%-5% of Dioscorea zingiberensis root extract, 1%-3% of Glycine max seed extract, 1%-3% of Pueraria lobata root extract, 1%-3% of Ginkgo biloba leaf extract, 1%-3% of Belamcanda chinensis root extract, 1%-3% of Rosa roxburghii fruit extract, 0.01%-0.5% of tetrahydropiperine, 2%-4% of isononyl isononanoate, 0.5%-2% of hydrogenated lecithin, 3%-8% of butylene glycol, 3-8% of glycerol, 3%-8% of caprylic / capric triglyceride, 1%-3% of jojoba seed oil, 1%-3% of cetearyl alcohol, 1%-3% of cetearyl glucoside, 0.1%-0.3% of carbomer, 0.05%-0.2% of xanthan gum and the balance of water.

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