A kind of blue salt moisturizing composition and preparation method thereof

Abstract

The invention relates to a daqin salt moisturizing composition and a preparation method thereof. The composition comprises the following ingredients: daqin salt and oligopeptides with whitening and anti-inflammatory functions. The daqin salt moisturizing composition of the invention adopts daqin salt as exfoliating particles instead of conventional scrub particles, and can kill bacteria and reduce inflammation, resist edema, and promote anti-inflammatory repair. The plant small molecule peptides in the composition have significant whitening and anti-inflammatory effects, can inhibit melanin production, brighten skin tone, even skin tone, and help repair damaged cells. The composition has good skin affinity, is mild and non-irritating, is suitable for various skin types, including sensitive skin, significantly improves skin quality, brightens skin tone, relieves inflammation, and deeply moisturizes, showing excellent skin care effects.

Description

Technical Field

[0001] The invention belongs to the field of cosmetics, and in particular relates to a blue salt moisturizing composition and a preparation method thereof. Background Art

[0002] Daqing salt, also known as Rong salt, Hu salt, and Qing salt, is a crystal of chloride-based halite mineral halite. It is mainly formed in dry salt basins and modern salt lakes, and is distributed in Inner Mongolia, Qinghai, Xinjiang, Tibet, Sichuan and other places. Daqing salt single crystals are cubic, multi-faceted, often connected together, in irregular blocks, and the particle size is generally 0.2-2.0 cm. Large particles can be seen with funnel-shaped growth remains, in irregular concave shapes, bluish white or dark white, translucent, fat-like luster, and some can be seen with unevenly distributed blue spots. The texture is hard and brittle, easy to smash, the cross section is clean, with a glass-like luster, a slight smell, and a salty taste. Large particles, hollow, cubic, dark white, and clean are preferred. It can be collected throughout the year, usually from June to August. After collection, it is taken out of the salt lake and dried for use. During processing, remove impurities from the original medicinal materials and crush them when used. Daqing salt is rich in a variety of trace elements needed by the human body. It has the effects of clearing heat, cooling blood, improving eyesight and moisturizing dryness. It is mainly used to clear heat and cool blood, treat symptoms such as vomiting blood, hematuria, swollen and bleeding gums, red and swollen eyes, and eye ulcers.

[0003] The application of oligopeptides in beauty products has attracted much attention due to its multiple functions. Oligopeptides are biological molecules composed of two or more amino acid residues connected by peptide bonds. They have relatively small molecular weights and strong skin permeability, and can play an important role on the skin surface and in the epidermis. An important function of oligopeptides is to resist free radical oxidation. Free radicals are one of the main causes of skin aging, which can damage skin cells and structures, leading to skin sagging, pigmentation and other problems. Oligopeptides can neutralize free radicals and protect the skin from oxidative damage, thereby delaying the skin aging process. In addition, whitening is another important role of oligopeptides. Some oligopeptides can inhibit the synthesis of melanin or decompose existing melanin, reduce the formation of skin spots, and achieve a whitening effect. Anti-edema and anti-inflammatory repair are the applications of oligopeptides in soothing and treating skin inflammation, reducing edema, and accelerating skin repair. Oligopeptides can promote cell regeneration and repair, and help treat inflammatory skin lesions such as acne and rashes.

[0004] People are paying more and more attention to the health and beauty of facial skin. Gel products, as skin care products with a jelly or gel texture as the main feature, have been widely recognized and applied in the beauty and skin care market in recent years. Gel products are refreshing, non-greasy, and easy to absorb, suitable for all skin types. In addition, exfoliation is increasingly gaining attention as an important step in skin care. The stratum corneum on the surface of the skin will accumulate in daily life due to factors such as environmental pollution, cosmetic residues, and its own metabolism, which will not only cause the skin surface to be rough and rough, but also hinder the skin's absorption and utilization of nutrients. Therefore, by regularly removing the stratum corneum, the skin's cell renewal and metabolism can be effectively promoted, restoring the skin to its natural state of smoothness and softness. In addition, the skin after exfoliation is not only more receptive to subsequent skin care products, but also helps reduce the occurrence of skin problems such as acne, pimples, and pigmentation, and improves the overall facial skin quality and health.

[0005] Exfoliating products mainly use abrasive particles to remove the aged stratum corneum on the surface of the skin, thereby restoring the skin to a smooth and tender state. Usually, the abrasive material can be a variety of different types of particles, such as silica particles, fruit core particles, salt particles or sugar particles. These particles can effectively remove dead skin cells on the surface of the skin, improve skin quality, and make the skin look cleaner and smoother. Patent CN108210432A of Henan Jiante Biotechnology Group Co., Ltd. discloses a scrub containing brown sugar, which has the effect of whitening, removing yellowness and exfoliating, and is gentle and non-irritating.

[0006] However, solid sugars (such as sucrose, fucoidan, and rhamnose) in scrubs may serve as a source of nutrition for bacteria. The energy provided by sugars may promote the growth and reproduction of bacteria, especially Propionibacterium acnes, which causes acne. These bacteria multiply in the pores of the skin, causing inflammation and the formation or aggravation of acne. In addition, traditional exfoliating products generally lack anti-inflammatory, moisturizing, and whitening effects.

[0007] In order to overcome the deficiencies of the prior art, the present invention provides a daqing salt moisturizing composition. The exfoliating particles in the moisturizing composition use daqing salt instead of conventional scrub particles, and small molecule peptides extracted from plants are added. The advantages are that it can kill bacteria and reduce inflammation, whiten, resist edema, and promote anti-inflammatory repair. In addition, the moisturizing composition has good skin affinity, is mild and non-irritating, has a higher acceptance rate among subjects, and is suitable for various skin types, including sensitive skin. Summary of the invention

[0008] The purpose of the invention is to provide a blue salt moisturizing composition which can kill bacteria, reduce inflammation, whiten skin, resist edema and promote anti-inflammatory repair.

[0009] Based on the above objectives, this application provides the following technical solutions:

[0010] The present invention provides an oligopeptide extracted from plumeria and having whitening and anti-inflammatory functions. The amino acid sequence of the oligopeptide is shown in SEQ ID No. 1, namely LCTGSPFK.

[0011] The present invention also provides a cyan salt moisturizing composition, which comprises cyan salt and an oligopeptide with whitening and anti-inflammatory functions, wherein the amino acid sequence of the oligopeptide is shown in SEQ ID No. 1, namely LCTGSPFK.

[0012] Daqing salt can be taken orally, applied externally or washed externally, and is suitable for a variety of skin problems. Daqing salt has uniform and fine particles, which can effectively remove dead skin cells on the skin surface, promote metabolism, and make the skin glow with health. Its natural bactericidal effect can effectively inhibit the growth of bacteria on the skin surface and prevent skin problems caused by bacteria, such as acne and blackheads. The mineral components in Daqing salt have excellent anti-inflammatory effects, which can soothe skin inflammation, reduce redness, swelling and sensitivity. Compared with some chemical exfoliants, Daqing salt is mild and non-irritating, suitable for all skin types, including sensitive skin.

[0013] Plumeria is a plant with many medicinal and cosmetic benefits. The small molecule peptides in plumeria extract have become an important ingredient in beauty products due to their excellent whitening, anti-inflammatory and free radical scavenging effects. These small molecule peptides can not only inhibit the formation of melanin, but also reduce skin inflammation, promote skin health and delay aging. The small molecule peptides in plumeria work through multiple mechanisms, providing strong support for the efficacy of skin care products.

[0014] In the present invention, a preferred embodiment is that the blue salt moisturizing composition further comprises a thickener, a moisturizer, and a preservative.

[0015] In the present invention, a preferred embodiment is that the thickener is selected from one or more of xanthan gum, hydroxyethyl cellulose, carbomer and carrageenan.

[0016] In the present invention, a preferred embodiment is that the moisturizing agent is selected from one or more of glycerin, hyaluronic acid, and propylene glycol.

[0017] In the present invention, in a preferred embodiment, every 100 parts by weight of the moisturizing exfoliating gel mask is composed of the following raw materials: 4-8 parts of blue salt; 2-8 parts of oligopeptides with whitening and anti-inflammatory functions; 0.5-3.5 parts of thickener; 1-4 parts of moisturizer; 0.8-1.5 parts of preservative; and the balance of deionized water.

[0018] The present invention also provides a method for preparing the blue salt moisturizing composition, comprising the following steps:

[0019] (1) Weigh the thickener and humectant, add them into a beaker filled with distilled water, heat in a water bath at 80-85°C, stir while heating, and let stand to allow them to fully swell to obtain gel 1, which is set aside for later use;

[0020] (2) Add part of the water, preservatives, and oligopeptides with whitening and anti-inflammatory functions into an emulsifying pot, heat and stir until completely dissolved, and obtain solution 1;

[0021] (3) Add solution 1 to gel 1 and stir at room temperature until fully blended. Then add blue salt and stir evenly.

[0022] The invention provides an application of the blue salt moisturizing composition in the field of whitening, anti-inflammatory and moisturizing cosmetics.

[0023] The invention provides a whitening, anti-inflammatory and moisturizing cosmetic prepared from the blue salt moisturizing composition.

[0024] In the present invention, in a preferred embodiment, the cosmetic is a moisturizing exfoliating gel mask.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] (1) The present invention uses blue salt as a scrub component. This avoids the technical problem that sugar components promote bacterial growth and reproduction and increase the risk of skin inflammation, thereby reducing the risk of infection and inflammation. Blue salt is mild and non-irritating, and also has natural bactericidal, anti-inflammatory and heat-removing effects. Its mineral components help relieve skin inflammation, reduce redness, swelling and irritation, and are particularly suitable for sensitive skin that is prone to inflammation. This feature enables blue salt to provide additional skin care effects while scrubbing, effectively improving skin health.

[0027] (2) The frangipani oligopeptide in the composition of the present invention has significant whitening and anti-inflammatory effects. The oligopeptide can inhibit the production of melanin, brighten the skin tone, and even out the skin tone. At the same time, its anti-inflammatory properties help to reduce skin inflammation and repair damaged cells. The synergistic effect with the blue salt makes the composition of the present invention significantly superior to the existing products with a single function in terms of skin care effect.

[0028] In summary, the composition of the present invention shows remarkable superiority in improving skin quality, brightening skin tone, relieving inflammation and deep moisturizing, and is significantly superior to the prior art. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0030] Figure 1 This application discloses the inhibitory effect of frangipani oligopeptide on melanin synthesis.

[0031] Figure 2 The present invention is to apply the anti-inflammatory effect of frangipani oligopeptides. DETAILED DESCRIPTION

[0032] In order to more fully understand the technical content of the present invention, the present invention will be further introduced and illustrated in conjunction with specific embodiments below; obviously, the embodiments described below are only part of the embodiments of the present invention, rather than all of the embodiments; based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present invention.

[0033] Example 1 Preparation of Plumeria crude oligopeptide extract

[0034] Fresh Plumeria petals were collected, cleaned and dried. 100.0 g of Plumeria was soaked in 300 mL of n-hexane for 15 min, stirred every 5 min with a HY-2 vortex mixer (Shanghai, China), and the filtrate was discarded. 300 mL of ethanol was added and refluxed for 30 min. The extract was evaporated to dryness at 40°C, dissolved with appropriate amount of water, and liquid-liquid extracted with dichloromethane, methyl acetate, ethyl acetate and butyl acetate in sequence (5 times of each solvent, 200 mL each time). The organic phase was collected and the organic solvent was removed by rotary evaporation at 40°C. The aqueous phase was filtered through a FlowMem0021-HP triple high-pressure flat membrane filtration device (Xiamen, Fujian), and the fraction with a molecular weight greater than 500 Da was collected and freeze-dried, and stored at -20°C before use.

[0035] Before sequencing, the lyophilized oligopeptide powder was dissolved in 0.1% trifluoroacetic acid (TFA) aqueous solution. The oligopeptide was sequenced using a mass spectrometer (LC-MS / MS). The mass-to-charge ratio (m / z) data of the oligopeptide was obtained by mass spectrometry, and secondary mass spectrometry (MS / MS) fragmentation analysis was performed to determine the amino acid sequence. The mass spectrometry data was processed using the mass spectrometry data analysis software Mascot, and the matching amino acid sequence was shown in SEQ ID No. 1, i.e., LCTGSPFK.

[0036] Example 2: Whitening and anti-inflammatory effects of frangipani oligopeptides

[0037] (1) Inhibitory effect on melanin synthesis

[0038] Mouse melanoma B16 cells were selected, and DMEM medium containing 10% fetal bovine serum was added and cultured in a 37°C, 5% CO2 humidity incubator. B16 cells in the logarithmic growth phase were taken and inoculated in T25 cell culture flasks, with an appropriate number of cells inoculated in each flask. The cells were cultured overnight in the culture flask to ensure attachment and adaptation. Prepare oligopeptide solutions of different concentrations, with concentrations of 0 mM, 0.8 mM, 1.6 mM, 3.2 mM, and 6.4 mM, respectively. These oligopeptide solutions were added to the cell culture flasks, respectively, and the untreated group was used as the cell control group. After 48 hours of culture, the cells were washed once with PBS. The cell culture flask was placed in an 80°C water bath for 30 minutes. The supernatant was collected and the absorbance value was measured at a wavelength of 475 nm.

[0039] The relative melanin content was calculated using the following formula: relative melanin content = (measured absorbance value - absorbance value of blank control group) / (absorbance value of cell control group - absorbance value of blank control group) × 100%.

[0040] The experimental results are as follows Figure 1 As shown, the melanin content of B16 cells added with oligopeptide solutions of different concentrations is shown in the following table:

[0041] Table 1 Inhibitory effect of frangipani oligopeptides on melanin synthesis

[0042] Concentration (mM) Absorbance (475 nm) Relative melanin content (%) 0 (Control Group) 1.000 100 0.8 0.850 85 1.6 0.700 70 3.2 0.500 50 6.4 0.300 30

[0043] The results showed that the oligopeptide solution could significantly inhibit the melanin synthesis of B16 cells at all concentrations, and showed a concentration-dependent manner. As the concentration of oligopeptides increased, the melanin content decreased significantly, indicating that the oligopeptides had a good whitening effect.

[0044] (2) Determination of anti-inflammatory activity of oligopeptides in frangipani extract

[0045] Tumor necrosis factor-α (TNF-α) was measured by enzyme-linked immunosorbent assay (ELISA). Tumor necrosis factor-α (TNF-α) is a common inflammatory factor, and its concentration can be used as an indicator of cellular inflammatory status, immune response intensity, and related biological processes.

[0046] RAW 264.7 cells were seeded in 24-well plates (2 × 10 5cells / well), and then incubated for 12 h. Different concentrations of oligopeptide solutions were added and incubated for 2 h. Subsequently, LPS (1 μg / mL) was added to the cells and incubated for another 24 h. The cell culture supernatant was collected and the levels of inflammatory cytokines were determined according to the instructions in the ELISA kit. DXMS (50 μg / mL) was used as a positive control.

[0047] like Figure 2 As shown in the figure, in the LPS-stimulated untreated group (-), TNF-α was significantly increased, confirming that the inflammatory model was successfully constructed. In addition, after treatment with crude oligopeptide extracts at different concentrations (50, 100 and 200 μg / mL), TNF-α was significantly reduced, and the frangipani oligopeptide-administered group significantly inhibited the secretion of TNF-α proinflammatory cytokines, and both showed concentration dependence. It is worth noting that the oligopeptide is very effective in reducing TNF-α, and its inhibitory effect of 100 μg / mL is close to that of the positive drug group (DXMS). In summary, frangipani oligopeptides can inhibit LPS-induced cellular TNF-α secretion and have certain anti-inflammatory activity.

[0048] Example 3 Preparation of Daqing Salt Moisturizing Exfoliating Gel Mask

[0049] Weigh 0.5 g of carbomer and 4 g of hyaluronic acid, add to a beaker containing 40 ml of distilled water, heat in a water bath at 80°C, stir while heating, and let stand to allow it to fully swell to obtain gel 1 for standby use; add 42.7 ml of distilled water, 0.8 g of paraben, and 8 g of frangipani oligopeptide to an emulsifying pot, heat and stir until completely dissolved to obtain solution 1; add solution 1 to gel 1, stir at room temperature until fully blended, then add 4 g of blue salt and stir evenly.

[0050] Example 4 Human skin test - Daqing salt moisturizing exfoliating gel mask

[0051] This example evaluates the safety of the isatine salt moisturizing exfoliating gel mask containing isatine salt and frangipani oligopeptide in Example 3 on the skin of the test subjects, and verifies its safety by observing the reaction.

[0052] Subject selection: A total of 100 subjects, including 50 females and 50 males. The age range is between 18 and 50 years old, and they are randomly divided into two groups. The subjects have healthy skin and no history of skin diseases and allergies. They meet the standards of the "Cosmetic Hygiene Standards" (2007 Edition). The subjects are voluntary and have confirmed that there are no contraindications related to the experiment.

[0053] Take 3g of the sample to be tested and add it to the chamber of the patch test tape. Immediately apply the patch test tape with the sample to the normal skin of the subject's left forearm from the bottom, press the chambers lightly one by one to expel the air and distribute the sample evenly. Mark the test site. Apply the patch once every 24 hours. After removing the patch, check according to the terminology of the CTFA guidelines half an hour later. A total of 5 patch tests were performed. An additional test was performed on the 6th day after the patch was applied to observe whether there was a delayed reaction.

[0054] In the observation during the patch test, all 100 subjects who applied the Daqing salt moisturizing exfoliating gel mask in Example 3 did not observe adverse reactions specified in the "Technical Specifications for Safety of Cosmetics" (2015 Edition) Part V Human Skin Closed Patch Test Skin, such as obvious irritation reactions such as erythema, itching, irritation, etc., during the patch period and in the additional inspection after the patching. This shows that the facial gel mask containing Daqing salt and oligopeptides from plumeria extract has low irritation to the skin of the subjects under the conditions tested. These results provide important support for the skin safety of the facial gel mask as a cosmetic ingredient or drug auxiliary ingredient.

[0055] Example 5: Moisturizing effect test of Daqing Salt Moisturizing Exfoliating Gel Mask

[0056] The moisturizing efficacy of Example 3 and the blank group (deionized water) was tested: 100 subjects, including 50 females and 50 males. The age range was between 18 and 50 years old, and they were randomly divided into two groups. The subjects had healthy skin and had no history of skin diseases and allergies. It met the standards of the "Cosmetic Hygiene Standards" (2007 edition). The experiment was carried out at room temperature (22±1)℃ and humidity 40%-60%. Before the experiment, the volunteers had to stay quiet for at least 30 minutes in this environment. After the environment reached equilibrium, a 3cm×3cm area was drawn on the left and right forearms, with an interval of at least 1cm. The sample was taken at (2.0±0.1)mg / cm 2 The dose was applied to the test area. After the subjects used the cosmetics for 5 minutes, 30 minutes, and 6 hours, the technicians used the skin moisture test probe to test the moisture content of the test area. The average value of 5 measurements was taken as the test value. The test results are shown in Table 2. The higher the moisture value change rate, the better the moisturizing effect.

[0057] The formula for calculating the moisture value change rate is as follows:

[0058] Moisture content change rate = (moisture content after use - moisture content before use) / moisture content before use * 100%

[0059] Table 2 Moisturizing test results of Daqing Salt Moisturizing Exfoliating Gel Mask

[0060]

[0061] It can be seen that the Daqing Salt Moisturizing Exfoliating Gel Mask of Example 3 has a good moisturizing effect. Five minutes after application, the moisture content of the skin surface layer is significantly improved. And after half an hour, it still has a good moisturizing effect. Therefore, this product can be used as a beauty and skin care product to improve the phenomenon of dry skin and peeling.

[0062] Based on the above description, it will be appreciated by those skilled in the art that the present disclosure may be implemented in different specific forms without changing its technical spirit and essential features. Therefore, it should be understood that the above-mentioned embodiments are not restrictive in all aspects, but illustrative. The scope of the present disclosure is limited by the appended claims, rather than by the preceding description thereof, and therefore all changes and modifications fall within the boundaries and scope of the claims, or the equivalents of such boundaries and scope are therefore intended to be covered by the claims.

Claims

1. A blue salt moisturizing composition, characterized in that: The cyan salt moisturizing composition comprises cyan salt, a thickener, a moisturizer, a preservative, and an oligopeptide with whitening and anti-inflammatory functions, wherein the amino acid sequence of the oligopeptide is shown in SEQ ID No. 1; Every 100 parts by weight of the daqing salt moisturizing composition is composed of the following raw materials: 4-8 parts of daqing salt; 2-8 parts of oligopeptides with whitening and anti-inflammatory functions; 0.5-3.5 parts of thickener; 1-4 parts of moisturizer; 0.8-1.5 parts of preservative; and the balance of deionized water.

2. The blue salt moisturizing composition according to claim 1, characterized in that The thickener is selected from one or more of xanthan gum, hydroxyethyl cellulose, carbomer and carrageenan.

3. The blue salt moisturizing composition according to claim 1, characterized in that The moisturizing agent is selected from one or more of glycerin, hyaluronic acid and propylene glycol.

4. The blue salt moisturizing composition according to claim 1, characterized in that The preservative is selected from one or more of ethylhexylglycerin and paraben.

5. A method for preparing the blue salt moisturizing composition according to any one of claims 1 to 4, characterized in that: The following steps are involved: (1) Weigh the thickener and humectant, add them into a beaker filled with distilled water, heat in a water bath at 80-85°C, stir while heating, and let stand to allow them to fully swell to obtain gel 1, which is set aside for later use; (2) Add part of the water, preservatives, and oligopeptides with whitening and anti-inflammatory functions into an emulsifying pot, heat and stir until completely dissolved, and obtain solution 1; (3) Add solution 1 to gel 1 and stir at room temperature until fully blended. Then add blue salt and stir evenly.

6. Use of the Daqing salt moisturizing composition according to any one of claims 1 to 4 or the Daqing salt moisturizing composition prepared by the preparation method of claim 5 in preparing cosmetics with whitening, anti-inflammatory and moisturizing effects.

7. A whitening, anti-inflammatory and moisturizing cosmetic prepared from the Daqing salt moisturizing composition according to any one of claims 1 to 4 or the Daqing salt moisturizing composition prepared by the preparation method of claim 5.

8. The cosmetic according to claim 7, which is a moisturizing exfoliating gel mask.

Images