Formula food for regulating intestinal tract and preparation method thereof

Abstract

The present invention relates to the field of health food, and in particular to a formula food for regulating the intestine and a preparation method thereof. The formula food for regulating the intestine contains raspberry extract, oligofructose, alpha cyclodextrin, cyclodextrin-modified soy protein isolate, rice protein, yam powder, konjac powder, sesame powder, L-sodium ascorbate, and whole milk powder. The formula food for regulating the intestine of the present invention has a synergistic effect between raspberry extract and oligofructose with a specific composition, and alpha cyclodextrin and cyclodextrin-modified soy protein isolate have a synergistic effect, which can effectively regulate intestinal flora, increase the abundance of beneficial bacteria, and reduce the number of harmful bacteria, and can prevent and improve constipation, indigestion, etc., which is conducive to maintaining intestinal health. And the preparation method of the formula food for regulating the intestine of the present invention is simple, and the way of taking is simple and convenient, and has great application prospects.

Description

Technical Field

[0001] The present invention relates to the technical field of health food, in particular to a formula food for regulating intestinal tract and a preparation method thereof. Background Art

[0002] With the acceleration of the pace of modern life, people's dietary structure and habits have changed significantly, which has led to an increase in the incidence of intestinal dysfunction year by year. As an important digestive and absorptive organ of the human body, the intestine is not only responsible for the digestion of food and the absorption of nutrients, but also for the excretion of waste. Its health status directly affects the overall health of the human body.

[0003] Intestinal dysfunction, also known as enteric neurosis, is a general term for a series of gastrointestinal syndromes. This disease is mainly manifested by abnormal gastrointestinal motility and secretory function, but is not accompanied by organic histological lesions. The main causes of intestinal dysfunction are as follows: First, it is related to the imbalance of intestinal flora. In the human intestine, there are about 400 kinds of bacteria coexisting, forming the intestinal flora. These bacteria include beneficial bacteria and harmful bacteria. They check and balance each other in a healthy state and jointly maintain the balance of microecology in the intestine, which plays a vital role in human health. However, under the influence of various factors, the number of probiotics in the intestine may decrease, while harmful bacteria may over-proliferate, leading to an imbalance of intestinal microecology, which in turn causes clinical symptoms such as diarrhea. Secondly, mental factors also play an important role. For example, excessive fatigue, emotional tension, and stress in life and work may interfere with the normal function of the brain and disrupt the coordination of the brain-gut axis, thereby leading to gastrointestinal dysfunction. In addition, irregular eating habits and adverse stimuli such as frequent use of laxatives or enemas may also promote the development of intestinal dysfunction.

[0004] Currently, there are many kinds of foods on the market for regulating intestinal function. However, these foods on the market currently have some limitations: some products have complex ingredients that are difficult to obtain or prepare completely; others may contain drug ingredients that may bring potential toxic side effects. In addition, these foods are not effective in regulating intestinal flora, and the significance and stability of their effects need to be further improved. Summary of the invention

[0005] The purpose of the present invention is to provide a formula food for regulating the intestine and a preparation method thereof, which can effectively regulate the intestinal flora and improve intestinal function, so as to solve the problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A formula food for regulating the intestine, comprising the following components:

[0008] 5-8 parts of raspberry extract, 10-15 parts of oligofructose, 4-8 parts of alpha-cyclodextrin, 10-15 parts of cyclodextrin-modified soy protein isolate, 10-15 parts of rice protein, 20-30 parts of yam powder, 20-25 parts of konjac powder, 15-20 parts of sesame powder, 0.5-0.8 parts of L-sodium ascorbate, 4-8 parts of whole milk powder, and the above components are proportioned by weight; the mass proportions of trisaccharides, tetrasaccharides, pentasaccharides and hexasaccharides in the oligofructose are 30-33%, 40-44%, 18-24% and 6-10% respectively; the composition of the raspberry extract comprises polysaccharides, proteins, arabinose, galactose, glucose and galacturonic acid;

[0009] The preparation method of the raspberry extract is as follows:

[0010] Step 1, crush raspberry fruits into powder, add the raspberry powder into an appropriate amount of water and soak to obtain a raspberry water extract;

[0011] Step 2, heating the water extract containing raspberries at a temperature of 60-80° C., and then filtering to obtain a filtrate;

[0012] Step 3, concentrating and freeze-drying the filtrate to obtain a powdered raspberry extract;

[0013] The preparation method of the intestinal tract regulating formula food comprises the following steps:

[0014] S1. Weigh each raw material powder according to the ratio;

[0015] S2, mix the raspberry extract and oligofructose evenly, sieve and set aside;

[0016] S3, mixing α-cyclodextrin and cyclodextrin-modified soy protein isolate uniformly, sieving, and setting aside;

[0017] S4, the remaining rice protein, yam powder, konjac powder, sesame powder, L-sodium ascorbate, whole milk powder are mixed, sieved, and set aside;

[0018] S5, mixing the materials obtained in S2-S4 uniformly to obtain a powder;

[0019] In S2-S4, the sieving is through a 40-50 mesh sieve.

[0020] Furthermore, the intestinal regulating formula food is composed of the following components: 5-7 parts of raspberry extract, 12-15 parts of oligofructose, 6-8 parts of α-cyclodextrin, 10-12 parts of cyclodextrin-modified soy protein isolate, 12-15 parts of rice protein, 20-25 parts of yam powder, 22-25 parts of konjac powder, 15-18 parts of sesame powder, 0.5-0.6 parts of L-sodium ascorbate, and 5-7 parts of whole milk powder, and the above components are proportioned by weight; the mass proportions of trisaccharides, tetrasaccharides, pentasaccharides, and hexasaccharides in the oligofructose are 30-33%, 40-44%, 18-24%, and 6-10%, respectively; the composition of the raspberry extract includes polysaccharides, proteins, arabinose, galactose, glucose, and galacturonic acid.

[0021] Furthermore, the intestinal regulating formula food is composed of the following components: 6 parts of raspberry extract, 13 parts of oligofructose, 7 parts of α-cyclodextrin, 11 parts of cyclodextrin-modified soy protein isolate, 13 parts of rice protein, 23 parts of yam powder, 22 parts of konjac powder, 16 parts of sesame powder, 0.6 parts of L-sodium ascorbate, and 6 parts of whole milk powder, and the above components are proportioned by weight; the mass proportions of trisaccharides, tetrasaccharides, pentasaccharides, and hexasaccharides in the oligofructose are 30-33%, 40-44%, 18-24%, and 6-10%, respectively; the composition of the raspberry extract includes polysaccharides, proteins, arabinose, galactose, glucose, and galacturonic acid.

[0022] Furthermore, the oligofructose is prepared by the following method:

[0023] Step 1, grinding the cleaned Jerusalem artichoke to obtain inulin;

[0024] Step 2, extracting inulin from inulin;

[0025] Step 3, the extracted inulin is hydrolyzed by enzyme catalysis to obtain a mixture, the hydrolysis temperature is 70-110°C;

[0026] Step 4, purifying the mixture obtained by hydrolysis by filtration, centrifugation or chromatography;

[0027] Step 5: Concentrate and dry the purified solution at a drying temperature of 70-80° C. to obtain powdered fructo-oligosaccharide.

[0028] On the other hand, the present invention also provides a method for preparing the above-mentioned intestinal regulating formula food, comprising the following steps:

[0029] S1. Weigh each raw material powder according to the ratio;

[0030] S2, mix the raspberry extract and oligofructose evenly, sieve and set aside;

[0031] S3, mixing α-cyclodextrin and cyclodextrin-modified soy protein isolate uniformly, sieving, and setting aside;

[0032] S4, the remaining rice protein, yam powder, konjac powder, sesame powder, L-sodium ascorbate, whole milk powder are mixed, sieved, and set aside;

[0033] S5. Mix the materials obtained in S2-S4 evenly to obtain powder.

[0034] Furthermore, in S2-S4, the sieving is through a 40-mesh sieve.

[0035] Furthermore, the powder obtained in S5 is prepared into granules.

[0036] Furthermore, the powder obtained in S5 is prepared into an oral liquid.

[0037] Compared with the prior art, the present invention has the following beneficial effects:

[0038] (1) The present invention provides a formula food for regulating the intestine. The formula food adopts specific components and their specific contents so that the various components in the formula food for regulating the intestine can cooperate with each other. While maintaining the balance of intestinal microecology, the abundance of beneficial bacteria in the intestine is further increased, the growth of harmful bacteria in the intestine is inhibited, the growth rate of beneficial bacteria is increased, and constipation, indigestion and the like are effectively prevented and improved, thereby maintaining intestinal health.

[0039] (2) The present invention provides a formula food for regulating the intestine, wherein the raw material formula comprises raspberry extract, wherein the raspberry extract comprises polysaccharides, proteins, arabinose, galactose, glucose and galacturonic acid, and has the function of regulating intestinal flora. The raw material formula further comprises oligofructose, wherein the oligofructose used in the present invention is prepared by hydrolyzing Jerusalem artichoke, wherein the mass proportions of trisaccharides, tetrasaccharides, pentasaccharides and hexasaccharides in the oligofructose are 30-33%, 40-44%, 18-24% and 6-10%, respectively, and the oligofructose itself has the function of moistening the intestine and promoting bowel movements and preventing and treating constipation. In addition, the oligofructose of a specific composition obtained by the present invention and the raspberry extract of a specific composition have a good synergistic effect in regulating intestinal flora, increasing the abundance of beneficial intestinal bacteria and reducing the number of harmful bacteria.

[0040] (3) The present invention provides a formula food for regulating the intestinal tract, wherein the raw material formula includes α-cyclodextrin, which can improve the intestinal flora and enhance the intestinal function. The raw material formula also includes cyclodextrin-modified soy protein isolate, which can provide rich nutrition and is more easily absorbed by the body, and α-cyclodextrin and cyclodextrin-modified soy protein isolate can synergistically regulate the intestinal flora and increase the growth rate of beneficial intestinal bacteria.

[0041] (4) The present invention provides a formula food for regulating the intestine. The raw material formula comprises yam powder, konjac powder and sesame powder, which has the effects of moistening the intestine and facilitating bowel movements, providing rich nutrition and improving the taste of food. The raw material formula also comprises whole milk powder, which can further improve the nutritional value and the taste of food. Rice protein is easily absorbed by the body, and the content of sodium L-ascorbate is relatively low, which makes the intestinal cleansing effect better.

[0042] (5) The present invention provides a method for preparing a formula food for regulating the intestine. The food preparation method adopted by the present invention is simple, and the administration method is simple and convenient, which can effectively reduce the manufacturing cost and has great application prospects.

[0043] (6) The intestinal regulating formula food provided by the present invention can effectively regulate the intestinal flora, increase the abundance of beneficial bacteria in the intestine, reduce the number of harmful bacteria, and increase the growth rate of beneficial bacteria. It can effectively prevent and improve constipation, indigestion, etc., and is beneficial to maintaining intestinal health. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0045] Figure 1 : A flow chart of the method for preparing the formula food for regulating the intestinal tract of the present invention;

[0046] Figure 2 : A flow chart of the preparation method of the raspberry extract of the present invention;

[0047] Figure 3 : Flow chart of the method for preparing oligofructose in the present invention;

[0048] Figure 4 :Statistical results of feces moisture content of each group of mice. DETAILED DESCRIPTION

[0049] The present invention is further explained below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention. After reading the present invention, various equivalent modifications of the present invention by those skilled in the art all fall within the scope defined by the claims attached to this application.

[0050] Embodiment 1:

[0051] A formula food for regulating the intestine, the raw materials and preparation method used are as follows:

[0052] The raw materials are:

[0053] 5 parts of raspberry extract,

[0054] 10 parts of oligofructose,

[0055] 4 parts of α-cyclodextrin,

[0056] 10 parts of cyclodextrin-modified soy protein isolate,

[0057] 10 servings of rice protein,

[0058] 20 portions of yam powder,

[0059] 20 portions of konjac flour,

[0060] 15 portions of sesame powder,

[0061] 0.5 parts of L-sodium ascorbate,

[0062] 4 parts whole milk powder,

[0063] The above components are mixed in proportions by weight.

[0064] The preparation method is:

[0065] refer to Figure 2 As shown, the preparation method of the raspberry extract comprises the following steps:

[0066] Step 1, crush raspberry fruits into powder, add the raspberry powder into an appropriate amount of water and soak to obtain a raspberry water extract;

[0067] Step 2, heating the water extract containing raspberries at a temperature of 60-80° C., and then filtering to obtain a filtrate;

[0068] Step 3: Concentrating and freeze-drying the filtrate to obtain powdered raspberry extract.

[0069] The prepared raspberry extract contains polysaccharide, protein, arabinose, galactose, glucose and galacturonic acid.

[0070] refer to Figure 3 As shown, the oligofructose is prepared by the following method:

[0071] Step 1, grinding the cleaned Jerusalem artichoke to obtain inulin;

[0072] Step 2, extracting inulin from inulin;

[0073] Step 3, the extracted inulin is hydrolyzed by enzyme catalysis to obtain a mixture, and the hydrolysis temperature is: 70-110°C;

[0074] Step 4, purifying the mixture obtained by hydrolysis by filtration, centrifugation or chromatography;

[0075] Step 5: Concentrate and dry the purified solution at a drying temperature of 70-80° C. to obtain powdered fructo-oligosaccharide.

[0076] The mass proportions of trisaccharides, tetrasaccharides, pentasaccharides and hexasaccharides in the prepared oligofructose are 30-33%, 40-44%, 18-24% and 6-10% respectively.

[0077] refer to Figure 1 As shown, the preparation method of the above-mentioned food for regulating intestinal flora comprises the following steps:

[0078] S1. Weigh each raw material powder according to the ratio;

[0079] S2, mixing the raspberry extract and oligofructose evenly, passing through a 40-mesh sieve, and setting aside;

[0080] S3, mixing α-cyclodextrin and cyclodextrin-modified soy protein isolate evenly, passing through a 40-mesh sieve, and setting aside;

[0081] S4, the remaining rice protein, yam powder, konjac powder, sesame powder, L-sodium ascorbate, whole milk powder are mixed, sieved, passed through a 40 mesh sieve, and standby;

[0082] S5. Evenly mix the materials obtained in S2-S4 to obtain powder.

[0083] Embodiment 2:

[0084] A formula food for regulating the intestine, the raw materials and preparation method used are as follows:

[0085] The raw materials are:

[0086] 8 parts of raspberry extract,

[0087] 15 parts of oligofructose,

[0088] 8 parts of α-cyclodextrin,

[0089] 15 parts of cyclodextrin-modified soy protein isolate,

[0090] 15 servings of rice protein,

[0091] 30 servings of yam powder,

[0092] 25 portions of konjac flour,

[0093] 20 portions of sesame powder,

[0094] 0.8 parts of L-sodium ascorbate,

[0095] 5 parts whole milk powder,

[0096] The above components are mixed in proportions by weight.

[0097] The preparation method is the same as that in Example 1.

[0098] Embodiment 3:

[0099] A formula food for regulating the intestine, the raw materials and preparation method used are as follows:

[0100] The raw materials are:

[0101] 5.5 parts of raspberry extract,

[0102] Oligofructose 11 parts,

[0103] 5 parts of α-cyclodextrin,

[0104] 12 parts of cyclodextrin-modified soy protein isolate,

[0105] 11 servings of rice protein,

[0106] 22 servings of yam powder,

[0107] 21 portions of konjac flour,

[0108] 17 parts sesame powder,

[0109] 0.5 parts of L-sodium ascorbate,

[0110] 5.5 parts of whole milk powder,

[0111] The above components are mixed in proportions by weight.

[0112] The preparation method is the same as that in Example 1.

[0113] Embodiment 4:

[0114] A formula food for regulating the intestine, the raw materials and preparation method used are as follows:

[0115] The raw materials are:

[0116] 7 parts of raspberry extract,

[0117] 12 parts of oligofructose,

[0118] 6 parts of α-cyclodextrin,

[0119] 13 parts of cyclodextrin-modified soy protein isolate,

[0120] 12 servings of rice protein,

[0121] 25 servings of yam powder,

[0122] 23 portions of konjac flour,

[0123] 18 portions of sesame powder,

[0124] 0.7 parts of L-sodium ascorbate,

[0125] 7 parts whole milk powder,

[0126] The above components are mixed in proportions by weight.

[0127] The preparation method is the same as that in Example 1.

[0128] Embodiment 5:

[0129] A formula food for regulating the intestine, the raw materials and preparation method used are as follows:

[0130] The raw materials are:

[0131] 7.5 parts of raspberry extract,

[0132] 14 parts of oligofructose,

[0133] 6.5 parts of α-cyclodextrin,

[0134] 14 parts of cyclodextrin-modified soy protein isolate,

[0135] 14 servings of rice protein,

[0136] 28 servings of yam powder,

[0137] 24 portions of konjac flour,

[0138] 19 portions of sesame powder,

[0139] 0.8 parts of L-sodium ascorbate,

[0140] 8 parts whole milk powder,

[0141] The above components are mixed in proportions by weight.

[0142] The preparation method is the same as that in Example 1.

[0143] Embodiment 6:

[0144] A formula food for regulating the intestine, the raw materials and preparation method used are as follows:

[0145] The raw materials are:

[0146] 6 parts of raspberry extract,

[0147] 13 parts of oligofructose,

[0148] 7 parts of α-cyclodextrin,

[0149] 11 parts of cyclodextrin-modified soy protein isolate,

[0150] 13 servings of rice protein,

[0151] 23 portions of yam powder,

[0152] 22 portions of konjac flour,

[0153] 16 portions of sesame powder,

[0154] 0.6 parts of L-sodium ascorbate,

[0155] 6 parts whole milk powder,

[0156] The above components are mixed in proportions by weight.

[0157] The preparation method is the same as that in Example 1.

[0158] Comparative Example 1:

[0159] A formula food for regulating the intestine, the raw materials and preparation method used are as follows:

[0160] The raw materials are:

[0161] Raspberry extract 0 parts,

[0162] 19 parts of oligofructose,

[0163] 7 parts of α-cyclodextrin,

[0164] 11 parts of cyclodextrin-modified soy protein isolate,

[0165] 13 servings of rice protein,

[0166] 23 portions of yam powder,

[0167] 22 portions of konjac flour,

[0168] 16 portions of sesame powder,

[0169] 0.6 parts of L-sodium ascorbate,

[0170] 6 parts whole milk powder,

[0171] The above components are mixed in proportions by weight.

[0172] The preparation method is:

[0173] The oligofructose is prepared by the following method:

[0174] Step 1, grinding the cleaned Jerusalem artichoke to obtain inulin;

[0175] Step 2, extracting inulin from inulin;

[0176] Step 3, the extracted inulin is hydrolyzed by enzyme catalysis to obtain a mixture, and the hydrolysis temperature is: 70-110°C;

[0177] Step 4, purifying the mixture obtained by hydrolysis by filtration, centrifugation or chromatography;

[0178] Step 5: Concentrate and dry the purified solution at a drying temperature of 70-80° C. to obtain powdered fructo-oligosaccharide.

[0179] The method for preparing the above-mentioned food for regulating intestinal flora comprises the following steps:

[0180] S1. Weigh each raw material powder according to the ratio;

[0181] S2, pass the oligofructose through a 40-mesh sieve for later use;

[0182] S3, mixing α-cyclodextrin and cyclodextrin-modified soy protein isolate evenly, passing through a 40-mesh sieve, and setting aside;

[0183] S4, the remaining rice protein, yam powder, konjac powder, sesame powder, L-sodium ascorbate, whole milk powder are mixed, sieved, passed through a 40 mesh sieve, and standby;

[0184] S5. Mix the materials obtained in S2-S4 evenly to obtain powder.

[0185] Comparative Example 2:

[0186] A formula food for regulating the intestine, the raw materials and preparation method used are as follows:

[0187] The raw materials are:

[0188] Raspberry extract 19 parts,

[0189] Oligofructose 0 parts,

[0190] 7 parts of α-cyclodextrin,

[0191] 11 parts of cyclodextrin-modified soy protein isolate,

[0192] 13 servings of rice protein,

[0193] 23 portions of yam powder,

[0194] 22 portions of konjac flour,

[0195] 16 portions of sesame powder,

[0196] 0.6 parts of L-sodium ascorbate,

[0197] 6 parts whole milk powder,

[0198] The above components are mixed in proportions by weight.

[0199] The preparation method is:

[0200] The preparation method of the raspberry extract comprises the following steps:

[0201] Step 1, crush raspberry fruits into powder, add the raspberry powder into an appropriate amount of water and soak to obtain a raspberry water extract;

[0202] Step 2, heating the water extract containing raspberries at a temperature of 60-80° C., and then filtering to obtain a filtrate;

[0203] Step 3: Concentrating and freeze-drying the filtrate to obtain powdered raspberry extract.

[0204] The method for preparing the above-mentioned food for regulating intestinal flora comprises the following steps:

[0205] S1. Weigh each raw material powder according to the ratio;

[0206] S2. Pass the raspberry extract through a 40-mesh sieve and set aside;

[0207] S3, mixing α-cyclodextrin and cyclodextrin-modified soy protein isolate evenly, passing through a 40-mesh sieve, and setting aside;

[0208] S4, the remaining rice protein, yam powder, konjac powder, sesame powder, L-sodium ascorbate, whole milk powder are mixed, sieved, passed through a 40 mesh sieve, and standby;

[0209] S5. Mix the materials obtained in S2-S4 evenly to obtain powder.

[0210] Comparative Example 3:

[0211] A formula food for regulating the intestine, the raw materials and preparation method used are as follows:

[0212] The raw materials are:

[0213] 6 parts of raspberry extract,

[0214] 13 parts of oligofructose,

[0215] α-cyclodextrin 0 parts,

[0216] 18 parts of cyclodextrin-modified soy protein isolate,

[0217] 13 servings of rice protein,

[0218] 23 portions of yam powder,

[0219] 22 portions of konjac flour,

[0220] 16 portions of sesame powder,

[0221] 0.6 parts of L-sodium ascorbate,

[0222] 6 parts whole milk powder,

[0223] The above components are mixed in proportions by weight.

[0224] The preparation method is:

[0225] The preparation method of the raspberry extract comprises the following steps:

[0226] Step 1, crush raspberry fruits into powder, add the raspberry powder into an appropriate amount of water and soak to obtain a raspberry water extract;

[0227] Step 2, heating the water extract containing raspberries at a temperature of 60-80° C., and then filtering to obtain a filtrate;

[0228] Step 3: Concentrating and freeze-drying the filtrate to obtain powdered raspberry extract.

[0229] The oligofructose is prepared by the following method:

[0230] Step 1, grinding the cleaned Jerusalem artichoke to obtain inulin;

[0231] Step 2, extracting inulin from inulin;

[0232] Step 3, the extracted inulin is hydrolyzed by enzyme catalysis to obtain a mixture, and the hydrolysis temperature is: 70-110°C;

[0233] Step 4, purifying the mixture obtained by hydrolysis by filtration, centrifugation or chromatography;

[0234] Step 5: Concentrate and dry the purified solution at a drying temperature of 70-80° C. to obtain powdered fructo-oligosaccharide.

[0235] The method for preparing the above-mentioned food for regulating intestinal flora comprises the following steps:

[0236] S1. Weigh each raw material powder according to the ratio;

[0237] S2, mixing the raspberry extract and oligofructose evenly, passing through a 40-mesh sieve, and setting aside;

[0238] S3, passing the cyclodextrin-modified soy protein isolate through a 40-mesh sieve for later use;

[0239] S4, the remaining rice protein, yam powder, konjac powder, sesame powder, L-sodium ascorbate, whole milk powder are mixed, sieved, passed through a 40 mesh sieve, and standby;

[0240] S5. Evenly mix the materials obtained in S2-S4 to obtain powder.

[0241] Comparative Example 4:

[0242] A formula food for regulating the intestine, the raw materials and preparation method used are as follows:

[0243] The raw materials are:

[0244] 6 parts of raspberry extract,

[0245] 13 parts of oligofructose,

[0246] 18 parts of α-cyclodextrin,

[0247] Cyclodextrin modified soy protein isolate 0 parts,

[0248] 13 servings of rice protein,

[0249] 23 portions of yam powder,

[0250] 22 portions of konjac flour,

[0251] 16 portions of sesame powder,

[0252] 0.6 parts of L-sodium ascorbate,

[0253] 6 parts whole milk powder,

[0254] The above components are mixed in proportions by weight.

[0255] The preparation method is:

[0256] The preparation method of the raspberry extract comprises the following steps:

[0257] Step 1, crush raspberry fruits into powder, add the raspberry powder into an appropriate amount of water and soak to obtain a raspberry water extract;

[0258] Step 2, heating the water extract containing raspberries at a temperature of 60-80° C., and then filtering to obtain a filtrate;

[0259] Step 3: Concentrating and freeze-drying the filtrate to obtain powdered raspberry extract.

[0260] The oligofructose is prepared by the following method:

[0261] Step 1, grinding the cleaned Jerusalem artichoke to obtain inulin;

[0262] Step 2, extracting inulin from inulin;

[0263] Step 3, the extracted inulin is hydrolyzed by enzyme catalysis to obtain a mixture, and the hydrolysis temperature is: 70-110°C;

[0264] Step 4, purifying the mixture obtained by hydrolysis by filtration, centrifugation or chromatography;

[0265] Step 5: Concentrate and dry the purified solution at a drying temperature of 70-80° C. to obtain powdered fructo-oligosaccharide.

[0266] The method for preparing the above-mentioned food for regulating intestinal flora comprises the following steps:

[0267] S1. Weigh each raw material powder according to the ratio;

[0268] S2, mixing the raspberry extract and oligofructose evenly, passing through a 40-mesh sieve, and setting aside;

[0269] S3, pass α-cyclodextrin through a 40-mesh sieve for later use;

[0270] S4, the remaining rice protein, yam powder, konjac powder, sesame powder, L-sodium ascorbate, whole milk powder are mixed, sieved, passed through a 40 mesh sieve, and standby;

[0271] S5. Evenly mix the materials obtained in S2-S4 to obtain powder.

[0272] Table 1 Ratio of raw materials for intestinal regulation formula food (unit: weight parts)

[0273] Raspberry Extract Fructo-oligosaccharides α-cyclodextrin Cyclodextrin modified soy protein isolate Rice protein Yam powder Konjac flour Sesame powder Sodium L-ascorbate Whole milk powder Example 1 5 10 4 10 10 20 20 15 0.5 4 Example 2 8 15 8 15 15 30 25 20 0.8 5 Example 3 5.5 11 5 12 11 22 21 17 0.5 5.5 Example 4 7 12 6 13 12 25 23 18 0.7 7 Example 5 7.5 14 6.5 14 14 28 24 19 0.8 8 Example 6 6 13 7 11 13 23 22 16 0.6 6 Comparative Example 1 0 19 7 11 13 23 22 16 0.6 6 Comparative Example 2 19 0 7 11 13 23 22 16 0.6 6 Comparative Example 3 6 13 0 18 13 23 22 16 0.6 6 Comparative Example 4 6 13 18 0 13 23 22 16 0.6 6

[0274] Verification example:

[0275] This example explores the effect of the intestinal regulating formula food of the present invention on improving constipation symptoms in mice:

[0276] (1) Experimental animals: Kunming mice, 18-22 g, were fed with regular granular rodent diet and purified water. The batch number of the experimental animal certificate is: SYXK(Lu)20180008.

[0277] (2) Animal grouping, modeling, and intervention methods

[0278] 140 mice were randomly divided into a blank control group, a model group, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, Example 6 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, a total of 12 groups; the blank control group consisted of 8 mice, and the remaining groups consisted of 12 mice.

[0279] Except for the blank control group, the other groups were orally administered loperamide (10 mg / kg) once a day for 10 consecutive days to induce constipation mouse models; the Example 1-6 groups used the corresponding intestinal regulating formula food described in Example 1-6 in Table 1, added water and stirred, and gavage once a day from the 11th day to the 20th day, the Comparative Example 1 Group to the Comparative Example 4 Group used the corresponding food in Table 1, added water and stirred, and gavage once a day from the 11th day to the 20th day, and the blank control group and the model group were gavaged with 10 mL / kg of normal saline.

[0280] SPSS 22.0 software was used for statistical analysis. The experimental data were expressed as mean ± standard deviation (n = 10). P < 0.05 was considered statistically significant.

[0281] (3) Analysis of water content in mouse feces

[0282] During the experiment, mouse feces were collected and the wet weight of feces (A) was recorded. After drying in a drying oven for 3 hours, the dry weight of feces (B) was recorded. Feces moisture content (%) = (AB) / A×100%. Figure 4 shown.

[0283] Depend on Figure 4 It can be seen that the feces moisture content of the blank control group mice was stable at about 66%, and the feces moisture content of the model group mice was about 52%. After consuming the intestinal regulation formula food for 10 days, compared with the model group mice, the feces moisture content of the mice in Example 1 to Example 6 groups increased significantly, and the feces moisture content of Example 6 group was the best, at about 62%. This shows that the intestinal regulation formula food involved in the present invention has a significant effect on restoring intestinal function.

[0284] The feces moisture content of mice in groups 1 to 4 of the comparative example increased less than that of mice in the model group. Figure 4 Shown in.

[0285] (4) Analysis of mouse intestinal flora

[0286] After the last oral gavage, collect an appropriate amount of mouse feces from each group, place them in a dry sterilized test tube, weigh them again, calculate the weight of the mouse feces, add 0.9% sodium chloride solution, stir and dissolve, prepare a 10 mg / mL suspension, dilute it 10 times, and inoculate it into each culture medium for cultivation. After the cultivation, count the colony forming units, and the results are expressed as the logarithm of the number of bacterial colonies per gram of feces to compare the changes in Lactobacillus, Bifidobacterium, Staphylococcus, and Proteus.

[0287] The test results are shown in Table 2. Compared with the blank control group, the number of lactobacillus and bifidobacterium in the feces of the model group mice significantly decreased, and the number of staphylococcus and proteus significantly increased. Compared with the model group, the feces of the mice of Example 1 group-Example 6 group of the formula food for regulating the intestinal tract of the present invention were consumed. The number of lactobacillus and bifidobacterium increased significantly, and the number of staphylococcus and proteus significantly decreased. Compared with the model group, the feces of the mice of Comparative Example 1 group-Comparative Example 2 group of the corresponding food in Table 1 were consumed. The increase in the number of lactobacillus and bifidobacterium was not obvious, and the reduction in the number of staphylococcus and proteus was not obvious. This shows that the formula food for regulating the intestinal tract involved in the present invention has a significant effect on regulating intestinal flora, increasing the abundance of beneficial bacteria in the intestinal tract, and reducing harmful bacteria in the intestinal tract. Referring to Table 2, compared with Comparative Example 1 and Comparative Example 2, the addition of raspberry extract and oligofructose prepared from Jerusalem artichoke to the food in Example 6 has a better technical effect of increasing the abundance of beneficial bacteria in the intestine and reducing harmful bacteria in the intestine than adding only raspberry extract or only adding oligofructose. It can be seen that there is a synergistic effect between the raspberry extract and the oligofructose prepared from Jerusalem artichoke in the formula food for regulating the intestine, and the simultaneous addition of the two can significantly increase the abundance of beneficial bacteria in the intestine and reduce harmful bacteria, and has a more significant technical effect of regulating intestinal flora.

[0288] Table 2 Effect of the composition of the present invention on the number of Lactobacillus, Bifidobacterium, Staphylococcus and Proteus in mice

[0289] Group n Lactobacilli Bifidobacterium staphylococcus Proteus Blank group 8 10.20±0.25 8.21±0.19 0.08+0.002 0.11±0.003 Model Group 12 3.65±0.18 2.17±0.12 8.91±0.16 9.10±0.21 Example 1 Group 12 <![CDATA[8.31±0.21 ab ]]> <![CDATA[6.65±0.28 ab ]]> <![CDATA[0.25±0.002 ab ]]> <![CDATA[0.22±0.004 ab ]]> Example 2 Group 12 <![CDATA[8.52±0.17 ab ]]> <![CDATA[7.60±0.22 ab ]]> <![CDATA[0.27±0.002 ab ]]> <![CDATA[0.21±0.002 ab ]]> Example 3 Group 12 <![CDATA[8.48±0.24 ab ]]> <![CDATA[7.62±0.25 ab ]]> <![CDATA[0.24±0.002 ab ]]> <![CDATA[0.24±0.003 ab ]]> Example 4 Group 12 <![CDATA[8.29±0.35 ab ]]> <![CDATA[7.51±0.16 ab ]]> <![CDATA[0.21±0.003 ab ]]> <![CDATA[0.20±0.002 ab ]]> Example 5 Group 12 <![CDATA[8.41±0.27 ab ]]> <![CDATA[7.34±0.22 ab ]]> <![CDATA[0.19±0.002 ab ]]> <![CDATA[0.20±0.003 ab ]]> Example 6 Group 12 <![CDATA[9.47±0.19 ab ]]> <![CDATA[7.78±0.35 ab ]]> <![CDATA[0.16±0.003 ab ]]> <![CDATA[0.19±0.002 ab ]]> Comparative Example 1 12 <![CDATA[6.23±0.12 b ]]> <![CDATA[5.05±0.09 b ]]> 0.32±0.002 <![CDATA[0.27±0.004 b ]]> Comparative Example 2 12 <![CDATA[6.47±0.12 b ]]> <![CDATA[5.21±0.13 b ]]> <![CDATA[0.29±0.003 b ]]> <![CDATA[0.26±0.002 b ]]>

[0290] Note: a Compared with the blank group, P<0.05; b Compared with the model group, P<0.01.

[0291] (5) Analysis of the proliferation rate of beneficial bacteria in the intestines of mice

[0292] Before gavage on the 11th day, the feces of the mice in the 6th group of embodiments and the feces of the mice in the 3rd and 4th groups of comparative examples were collected. After gavage on the 20th day, the feces of the mice in the 6th group of embodiments and the feces of the mice in the 3rd and 4th groups of comparative examples were collected, and each feces was placed in a dry sterilized test tube, weighed again, and the weight of the mouse feces was calculated. A 0.9% sodium chloride solution was added, stirred and dissolved, and a 10 mg / mL suspension was prepared, and then diluted 10 times, and inoculated in each culture medium for cultivation. After the cultivation was completed, the colony forming units were counted, and the results were expressed as the logarithmic value of the number of bacterial colonies per gram of feces, and the number of lactobacilli in the feces on the 11th day was recorded as a, and the number of bifidobacteria in the feces on the 11th day was recorded as c; the number of lactobacilli in the feces on the 20th day was recorded as b, and the number of bifidobacteria in the feces on the 11th day was recorded as d.

[0293] Lactobacillus proliferation rate = (ba) / a×100%;

[0294] Bifidobacterium proliferation rate = (dc) / c×100%;

[0295] The results are recorded in Table 3. From the data in Table 3, it can be seen that when α-cyclodextrin and cyclodextrin-modified soy protein isolate are simultaneously included in the food formula, the proliferation rate of beneficial bacteria is higher than that of only containing cyclodextrin or only containing cyclodextrin-modified soy protein isolate. It can be seen that there is a synergistic effect between α-cyclodextrin and cyclodextrin-modified soy protein isolate, which plays a role in increasing the proliferation rate of beneficial bacteria in the intestine.

[0296] Table 3 Effect of the composition of the present invention on the proliferation rate of Lactobacillus and Bifidobacterium in mice

[0297] Group n Lactobacillus proliferation rate Bifidobacterium proliferation rate Example 6 Group 12 159.45% 258.52% Comparative Example 3 12 97.53% 141.01% Comparative Example 4 12 118.63% 152.53%

[0298] In summary, the intestinal regulating formula food involved in the present invention has excellent effects of improving intestinal flora and enhancing intestinal function, raspberry extract and oligofructose prepared from Jerusalem artichoke have synergistic effects in increasing the efficacy, and α-cyclodextrin and cyclodextrin-modified soy protein isolate have significant synergistic effects in increasing the growth rate of beneficial bacteria.

[0299] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent measures for the raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0300] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0301] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A formula food for regulating the intestine, characterized in that: The invention is composed of the following components: 5-8 parts of raspberry extract, 10-15 parts of oligofructose, 4-8 parts of α-cyclodextrin, 10-15 parts of cyclodextrin-modified soy protein isolate, 10-15 parts of rice protein, 20-30 parts of yam powder, 20-25 parts of konjac powder, 15-20 parts of sesame powder, 0.5-0.8 parts of L-sodium ascorbate, and 4-8 parts of whole milk powder, wherein the above components are proportioned by weight; the mass proportions of trisaccharide, tetrasaccharide, pentasaccharide and hexasaccharide in the oligofructose are 30-33%, 40-44%, 18-24% and 6-10% respectively; the raspberry extract comprises polysaccharide, protein, arabinose, galactose, glucose and galacturonic acid; The preparation method of the raspberry extract is as follows: Step 1, crush raspberry fruits into powder, add the raspberry powder into an appropriate amount of water and soak to obtain a raspberry water extract; Step 2, heating the water extract containing raspberries at a temperature of 60-80° C., and then filtering to obtain a filtrate; Step 3, concentrating and freeze-drying the filtrate to obtain a powdered raspberry extract; The preparation method of the intestinal tract regulating formula food comprises the following steps: S1. Weigh each raw material powder according to the ratio; S2, mix the raspberry extract and oligofructose evenly, sieve and set aside; S3, mixing α-cyclodextrin and cyclodextrin-modified soy protein isolate uniformly, sieving, and setting aside; S4, the remaining rice protein, yam powder, konjac powder, sesame powder, L-sodium ascorbate, whole milk powder are mixed, sieved, and set aside; S5, mixing the materials obtained in S2-S4 uniformly to obtain a powder; In S2-S4, the sieving is through a 40-50 mesh sieve.

2. A formula food for regulating the intestine according to claim 1, characterized in that: The formula food for regulating the intestine is composed of the following components: 5-7 parts of raspberry extract, 12-15 parts of oligofructose, 6-8 parts of alpha-cyclodextrin, 10-12 parts of cyclodextrin-modified soy protein isolate, 12-15 parts of rice protein, 20-25 parts of yam powder, 22-25 parts of konjac powder, 15-18 parts of sesame powder, 0.5-0.6 parts of L-sodium ascorbate, and 5-7 parts of whole milk powder, wherein the above components are proportioned by weight; the mass proportions of trisaccharides, tetrasaccharides, pentasaccharides, and hexasaccharides in the oligofructose are 30-33%, 40-44%, 18-24%, and 6-10%, respectively; the composition of the raspberry extract comprises polysaccharides, proteins, arabinose, galactose, glucose, and galacturonic acid.

3. A formula food for regulating the intestine according to claim 2, characterized in that: The formula food for regulating the intestine is composed of the following components: 6 parts of raspberry extract, 13 parts of oligofructose, 7 parts of α-cyclodextrin, 11 parts of cyclodextrin-modified soy protein isolate, 13 parts of rice protein, 23 parts of yam powder, 22 parts of konjac powder, 16 parts of sesame powder, 0.6 parts of L-sodium ascorbate, and 6 parts of whole milk powder, and the above components are proportioned by weight; the mass proportions of trisaccharides, tetrasaccharides, pentasaccharides, and hexasaccharides in the oligofructose are 30-33%, 40-44%, 18-24%, and 6-10%, respectively; the composition of the raspberry extract includes polysaccharides, proteins, arabinose, galactose, glucose, and galacturonic acid.

4. A formula food for regulating the intestinal tract according to any one of claims 1 to 3, characterized in that: The oligofructose is prepared by the following method: Step 1, grinding the cleaned Jerusalem artichoke to obtain inulin; Step 2, extracting inulin from inulin; Step 3, the extracted inulin is hydrolyzed by enzyme catalysis to obtain a mixture, the hydrolysis temperature is 70-110°C; Step 4, purifying the mixture obtained by hydrolysis by filtration, centrifugation or chromatography; Step 5: Concentrate and dry the purified solution at a drying temperature of 70-80° C. to obtain powdered fructo-oligosaccharide.

5. A method for preparing a formula food for regulating the intestinal tract according to any one of claims 1 to 4, characterized in that: The steps include: S1. Weigh each raw material powder according to the ratio; S2, mix the raspberry extract and oligofructose evenly, sieve and set aside; S3, mixing α-cyclodextrin and cyclodextrin-modified soy protein isolate uniformly, sieving, and setting aside; S4, the remaining rice protein, yam powder, konjac powder, sesame powder, L-sodium ascorbate, whole milk powder are mixed, sieved, and set aside; S5. Mix the materials obtained in S2-S4 evenly to obtain powder.

6. The method for preparing a formula food for regulating the intestinal tract according to claim 5, characterized in that: In S2-S4, the sieving is through a 40-mesh sieve.

7. The method for preparing a formula food for regulating the intestinal tract according to claim 5, characterized in that: The powder obtained in S5 is prepared into granules.

8. The method for preparing a formula food for regulating the intestinal tract according to claim 5, characterized in that: The powder obtained in S5 is prepared into an oral solution.

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