A composition of Sophora japonica and Cistanche deserticola for improving bone and joint health, and its preparation method and application

Abstract

The present invention relates to the field of health care products, and in particular to a sophora japonica and cistanche composition for improving bone and joint health, and a preparation method and application thereof. The composition is composed of the following components in parts by weight: 3 to 6 parts of sophora japonica extract, 3 to 6 parts of cistanche extract, 1 to 5 parts of fungus powder, 1 to 3 parts of dried shrimp powder, 1 to 3 parts of dried plum powder, 1 to 3 parts of spinach powder, 1 to 3 parts of amaranth powder and 0.01 to 0.1 parts of vitamin D. Most of the components of the composition are medicinal and edible and daily dietary foods, which are easy to obtain, green and safe, and the combination of the specially treated components has the effect of synergistically inhibiting bone loss, increasing bone density and improving osteoblast activity, and improving bone and joint health.

Description

Technical Field

[0001] The invention belongs to the technical field of health care products, and in particular relates to a Sophora japonica and Cistanche deserticola composition for improving bone and joint health, and a preparation method and application thereof. Background Art

[0002] Modern people's living habits, such as students or office workers often sitting for long periods of time, looking down at mobile phones or computers, etc., and lack of exercise, have led to an increasing incidence of bone and joint problems among young people in recent years.

[0003] Students or office workers often suffer from shoulder pain, stiffness, and even limited shoulder movement. The reason is that the shoulder joint is not active enough, which can easily lead to symptoms such as osteoarthritis and osteoporosis over time.

[0004] Among them, osteoporosis (OP) is a chronic systemic bone disease that involves multiple organs and is characterized by abnormal bone metabolism, low bone mass, bone microstructure destruction, impaired bone strength and increased risk of brittle fractures under the long-term combined effects of multiple factors. It is accompanied by a high incidence rate, low awareness rate, and often causes serious complications. It is generally more common in the elderly, postmenopausal women, and people who take hormone drugs for a long time. The main clinical manifestations are back pain, limited mobility, spinal deformity, shortened height, hunchback, and easy fractures. These symptoms bring a lot of inconvenience to the patient's life, and are serious or even life-threatening, bringing a heavy economic burden to individuals, families, and society.

[0005] At present, most drugs commonly used to treat bone and joint health are Western medicine ingredients, such as bone resorption inhibitors and bone formation promoters. The former include bisphosphonates, estrogen, calcitonin, RANKL inhibitors, etc., and the latter include parathyroid hormone analogs. Generally, long-term medication is required, and the cost is expensive, and adverse reactions such as myalgia, joint pain, and gastrointestinal discomfort are prone to occur. In addition, there are some drugs that are mainly various calcium supplements, but the absorption of these products is limited, the effect is not obvious, and long-term consumption may also cause the risk of stones. Summary of the invention

[0006] In order to overcome the deficiencies of the prior art, the present invention provides a composition of Sophora japonica and Cistanche deserticola for improving bone and joint health and a preparation method thereof, wherein the composition comprises Sophora japonica extract, Cistanche deserticola extract, Fungus powder, Dried shrimp powder, Prune powder, Spinach powder, Amaranth powder and Vitamin D, which are mostly medicinal and edible and daily dietary foods, are easily available, and are green and safe. The specially treated components are combined to synergistically inhibit bone loss, increase bone density and improve osteoblast activity. Experimental verification has shown that the composition has rapid efficacy, no toxic side effects and no dependence.

[0007] The present invention is achieved through the following technical solutions:

[0008] The first aspect of the present invention provides a Sophora japonica seed and Cistanche deserticola composition for improving bone and joint health, the composition consisting of the following components in parts by weight: 3 to 6 parts of Sophora japonica seed extract, 3 to 6 parts of Cistanche deserticola extract, 1 to 5 parts of fungus powder, 1 to 3 parts of dried shrimp powder, 1 to 3 parts of dried plum powder, 1 to 3 parts of spinach powder, 1 to 3 parts of amaranth powder and 0.01 to 0.1 parts of vitamin D.

[0009] Preferably, in the above scheme, the preparation method of the sophora japonica extract is: uniformly dispersing the sophora japonica powder in Na2HPO4·12H2O-C6H8O7·H2O buffer, adding cellulase for enzymolysis, inactivating the enzyme, adjusting the pH value after cooling, and then adding papain for enzymolysis, adding cyclodextrin for embedding, inactivating the enzyme, filtering, and spray-drying the filtrate to obtain the sophora japonica extract.

[0010] The sophora japonica extract of the invention is firstly hydrolyzed by cellulase to destroy the plant cell wall and improve the leaching of effective substances; then hydrolyzed by papain to promote the effective components to become small molecules and improve the absorption rate; finally, cyclodextrin is added for embedding to improve the solubility and extraction rate of the effective substances, thereby improving the bioavailability of the effective components.

[0011] Preferably, the particle size of the sophora japonica flour is 40-80 mesh; the pH of the Na2HPO4·12H2O-C6H8O7·H2O buffer is between 4.8 and 5.2, and the mass ratio is 1:10-20 for the sophora japonica flour and the buffer; the amount of cellulase added is 0.2%-2% of the mass of the sophora japonica flour; the temperature for enzymolysis of the cellulase is 45°C-55°C, and the enzymolysis time is 1.5h-2.5h; the temperature for inactivating the enzyme is 80°C-90°C, and the time is 10min-30min; the adjustment The pH value ranges from 6.0 to 7.0; the added amount of the papain is 0.2% to 2% of the mass of the sophora japonica flour; the enzymatic hydrolysis temperature of the papain is 55° C. to 65° C.; and the enzymatic hydrolysis time is 1 h to 2 h; the cyclodextrin is one of α-cyclodextrin, β-cyclodextrin, and γ-cyclodextrin, and the added amount is 10% to 50% of the mass of the sophora japonica flour; the embedding temperature is 55° C. to 65° C.; and the enzymatic inactivation temperature is 80° C. to 90° C., and the time is 15 min to 30 min.

[0012] The pH can be adjusted by adding NaHCO3.

[0013] Preferably, the sophora japonica rice powder is obtained by breaking the wall, crushing and screening, and has a better extraction effect.

[0014] Preferably, the Cistanche deserticola extract is a desert type Cistanche deserticola extract.

[0015] Preferably, the preparation method of the Cistanche deserticola extract in the above scheme is: uniformly dispersing the Cistanche deserticola powder in Na2HPO4·12H2O-C6H8O7·H2O buffer, adding cellulase for enzymolysis, inactivating the enzyme, adjusting the pH value after cooling, adding glycoside hydrolase for enzymolysis, inactivating the enzyme, filtering after cooling, and spray drying the filtrate to obtain the Cistanche deserticola extract.

[0016] The Cistanche deserticola extract of the present invention is also firstly hydrolyzed by cellulase to help the extraction of effective components, and then hydrolyzed by glycoside hydrolase to convert the effective components therein from echinacoside to verbascoside with higher bioavailability.

[0017] Preferably, in the preparation method of the Cistanche deserticola extract, the particle size of the Cistanche deserticola powder is 40-80 mesh; the pH of the Na2HPO4·12H2O-C6H8O7·H2O buffer is between 4.8 and 5.2, and the mass ratio is Cistanche deserticola powder: buffer = 1:10-20; the amount of cellulase added is 0.5%-3% of the mass of the Cistanche deserticola powder, the temperature of the cellulase enzymolysis is 45°C-65°C, and the time is 1 h~2h; the temperature for inactivating the cellulase is 80℃~90℃, and the time is 10min~30min; the pH value is adjusted in the range of 6.0~7.0; the amount of glycoside hydrolase added is 1%~5% of the mass of Cistanche powder, the temperature for enzymolysis of the glycoside hydrolase is 40℃~50℃, and the time is 12h~18h; the temperature for inactivating the glycoside hydrolase is 80℃~90℃, and the time is 15min~30min.

[0018] The pH can be adjusted by adding NaHCO3.

[0019] Preferably, the Cistanche deserticola powder is obtained by drying, slicing, powdering and sieving the Cistanche deserticola. Preferably, the drying temperature is 45° C. to 60° C., and the drying time is 6 h to 12 h; and the slice thickness is 0.1 cm to 1 cm.

[0020] In a second aspect, the present invention also provides a method for preparing the above-mentioned Sophora japonica and Cistanche deserticola composition for improving bone and joint health, comprising the following steps:

[0021] Sophora japonica extract, Cistanche deserticola extract, fungus powder, dried shrimp powder, dried plum powder, spinach powder, amaranth powder and vitamin D are uniformly mixed according to weight proportions to obtain a composition.

[0022] In a third aspect, the present invention also provides a product for improving bone and joint health, comprising the above-mentioned Sophora japonica and Cistanche deserticola composition, wherein the product is a medicine, a food or a health product. The above-mentioned composition can be made into various forms of products by adding commonly used auxiliary materials, such as solid beverages, tablet candies, granules, capsules, etc.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] Sophora japonica is the dried buds and flowers of the leguminous plant Sophora japonica, also known as white Sophora japonica, Sophora japonica rice, and Sophora japonica seeds. It is rich in polysaccharides, flavonoids and isoflavones, saponins, volatile oils and other active compounds. Many studies have shown that Sophora japonica has antioxidant, anti-inflammatory, hypoglycemic, neuroprotective and other effects. After research, the inventor was surprised to find that Sophora japonica powder treated with enzymatic hydrolysis and embedding has good solubility, and has the effects of inhibiting bone loss, increasing bone density and improving osteoblast activity.

[0025] Cistanche deserticola is the fleshy stem with dried scaly leaves of Cistanche deserticola or Cistanche tubulosa, a plant of the Orobanchaceae family. It mainly contains phenylethanoid glycosides such as echinacoside and verbascoside, lignin components such as pinoresinol, and components such as alkaloids, sugars, sugar alcohols, sterols, and various trace elements, and has the functions of tonifying kidney yang, benefiting essence and blood, and moistening the intestines and relieving constipation. Studies have shown that echinacoside is poorly absorbed in the body and has extremely low bioavailability, while verbascoside has higher antioxidant capacity and higher bioavailability. The inventor has found through research that the content of verbascoside can be increased by treating Cistanche deserticola with cellulase and glycoside hydrolase. The Cistanche deserticola extract hydrolyzed by enzyme has a good effect of improving bone and joint health.

[0026] The applicant combines the two extracts with fungus powder, dried shrimp powder, dried plum powder, spinach powder, amaranth powder and vitamin D. These materials are all daily dietary foods, simple in composition and balanced in nutrition, rich in vitamins, calcium, etc., and can be eaten for a long time to improve the health of bone and joints. Furthermore, the composition of the present invention can be added with auxiliary materials commonly used in food, health products or medicines to make various forms of products. The obtained product has simple ingredients, has the effect of improving the health of bone and joints, has no toxic side effects, and can be eaten for a long time. DETAILED DESCRIPTION

[0027] The content of the present invention is further described below in conjunction with specific embodiments, but it should not be construed as limiting the present invention. If not specifically indicated, the technical means used in the embodiments are conventional means well known to those skilled in the art. Unless otherwise specified, the reagents and equipment used in the present invention are conventional products in the art.

[0028] Example 1

[0029] A Sophora japonica and Cistanche deserticola composition for improving bone and joint health, comprising 6 parts of Sophora japonica extract, 6 parts of Cistanche deserticola extract, 4 parts of fungus powder, 3 parts of dried shrimp powder, 3 parts of dried plum powder, 3 parts of spinach powder, 3 parts of amaranth powder and 0.1 parts of vitamin D in parts by weight.

[0030] Among them, the preparation method of the sophora japonica extract is as follows: crush the sophora japonica seeds with a wall breaking machine, pass through an 80-mesh sieve to obtain sophora japonica powder, evenly disperse the sophora japonica powder in a Na2HPO4·12H2O-C6H8O7·H2O buffer solution with a pH of 5.0 and a mass of 20 times that of the sophora japonica powder, add 2% of cellulase by mass of the sophora japonica powder, enzymolyze at 50°C for 2h, inactivate the enzyme at 85°C for 20min, add NaHCO3 to adjust the pH to 6.5 after cooling, add 2% of papain by mass of the sophora japonica powder, enzymolyze at 60°C for 2h, add 50% of α-cyclodextrin by mass of the sophora japonica powder, embed at 60°C for 1h, inactivate the enzyme at 85°C for 30min, filter while hot, and spray-dry the filtrate to obtain the sophora japonica extract.

[0031] The preparation method of the Cistanche deserticola extract is as follows: drying the desert type Cistanche deserticola at 55°C for 12h, cutting it into 0.1cm thin slices and grinding it into powder, sieving it through an 80-mesh sieve to obtain the Cistanche deserticola powder, uniformly dispersing the Cistanche deserticola powder in a Na2HPO4·12H2O-C6H8O7·H2O buffer solution with a pH value of 5.0 and a mass of 20 times that of the Cistanche deserticola powder, adding 3% of cellulase by mass of the Cistanche deserticola powder, enzymolysis at 55°C for 2h, inactivating the enzyme at 85°C for 20min, adding NaHCO3 to adjust the pH value to 6.5 after cooling, adding glycoside hydrolase at 5% by mass of the Cistanche deserticola powder, enzymolysis at 45°C for 18h, inactivating the enzyme at 85°C for 30min, filtering after cooling, and spray drying the filtrate to obtain the Cistanche deserticola extract.

[0032] According to the above-mentioned mass proportions, the Sophora japonica seed extract, Cistanche deserticola extract, Auricularia auricula powder, dried shrimp powder, dried plum powder, spinach powder, Amaranth powder and vitamin D are uniformly mixed to obtain the Sophora japonica seed and Cistanche deserticola composition for improving bone and joint health.

[0033] Example 2

[0034] A Sophora japonica and Cistanche deserticola composition for improving bone and joint health, comprising 6 parts of Sophora japonica extract, 3 parts of Cistanche deserticola extract, 5 parts of fungus powder, 3 parts of dried shrimp powder, 1 part of dried plum powder, 1 part of spinach powder, 1 part of amaranth powder and 0.1 part of vitamin D in parts by weight.

[0035] The preparation method of the sophora japonica extract is as follows: crush the sophora japonica seeds with a wall-breaking machine, sieve through a 60-mesh sieve to obtain sophora japonica powder, evenly disperse the sophora japonica powder in a Na2HPO4·12H2O-C6H8O7·H2O buffer solution with a pH of 5.0 and a mass of 10 times that of the sophora japonica powder, add 1% of cellulase by mass of the sophora japonica powder, hydrolyze at 55°C for 1h, inactivate the enzyme at 90°C for 10min, add NaHCO3 to adjust the pH to 6.5 after cooling, add 1% of papain by mass of the sophora japonica powder, hydrolyze at 65°C for 1h, add 30% of α-cyclodextrin by mass of the sophora japonica powder, embed at 65°C for 0.5h, inactivate the enzyme at 90°C for 15min, filter while hot, and spray-dry the filtrate to obtain the sophora japonica extract.

[0036] The preparation method of the Cistanche deserticola extract is as follows: drying the desert type Cistanche deserticola at 60°C for 6h, cutting into 0.5cm thin slices and grinding into powder, sieving through a 60-mesh sieve to obtain the Cistanche deserticola powder, uniformly dispersing the Cistanche deserticola powder in a Na2HPO4·12H2O-C6H8O7·H2O buffer solution with a pH value of 5.0 and a mass of 10 times that of the Cistanche deserticola powder, adding 2% of cellulase by mass of the Cistanche deserticola powder, enzymolysis at 60°C for 1h, inactivating the enzyme at 90°C for 10min, adding NaHCO3 to adjust the pH value to 6.5 after cooling, adding glycoside hydrolase at 3% by mass of the Cistanche deserticola powder, enzymolysis at 50°C for 12h, inactivating the enzyme at 90°C for 15min, filtering after cooling, and spray drying the filtrate to obtain the Cistanche deserticola extract.

[0037] According to the above-mentioned mass proportions, the Sophora japonica seed extract, Cistanche deserticola extract, Auricularia auricula powder, dried shrimp powder, dried plum powder, spinach powder, Amaranth powder and vitamin D are uniformly mixed to obtain the Sophora japonica seed and Cistanche deserticola composition for improving bone and joint health.

[0038] Example 3

[0039] A Sophora japonica and Cistanche deserticola composition for improving bone and joint health, comprising 3 parts of Sophora japonica extract, 6 parts of Cistanche deserticola extract, 3 parts of fungus powder, 2 parts of dried shrimp powder, 2 parts of dried plum powder, 2 parts of spinach powder, 2 parts of amaranth powder and 0.05 parts of vitamin D in parts by weight.

[0040] Among them, the preparation method of the sophora japonica extract is as follows: crush the sophora japonica seeds with a wall-breaking machine, pass through a 40-mesh sieve to obtain sophora japonica powder, evenly disperse the sophora japonica powder in a Na2HPO4·12H2O-C6H8O7·H2O buffer solution with a pH of 5.0 and a mass of 15 times that of the sophora japonica powder, add 1.5% of cellulase by mass of the sophora japonica powder, enzymolysis at 45°C for 2.5 hours, inactivate the enzyme at 80°C for 30 minutes, add NaHCO3 to adjust the pH to 6.5 after cooling, add 1.5% of papain by mass of the sophora japonica powder, enzymolysis at 55°C for 2 hours, add 50% of γ-cyclodextrin by mass of the sophora japonica powder, embed at 65°C for 1 hour, inactivate the enzyme at 80°C for 30 minutes, filter while hot, and spray-dry the filtrate to obtain the sophora japonica extract.

[0041] The preparation method of the Cistanche deserticola extract is as follows: drying the desert type Cistanche deserticola at 45°C for 12h, cutting into 0.8cm slices and powdering, sifting through a 40-mesh sieve to obtain the Cistanche deserticola powder, uniformly dispersing the Cistanche deserticola powder in a Na2HPO4·12H2O-C6H8O7·H2O buffer solution with a pH value of 5.0 and a mass of 15 times that of the Cistanche deserticola powder, adding 1% of cellulase by mass of the Cistanche deserticola powder, enzymolysis at 45°C for 2h, inactivating the enzyme at 80°C for 30min, adding NaHCO3 to adjust the pH value to 6.5 after cooling, adding glycoside hydrolase at 3% by mass of the Cistanche deserticola powder, enzymolysis at 40°C for 18h, inactivating the enzyme at 80°C for 30min, filtering after cooling, and spray drying the filtrate to obtain the Cistanche deserticola extract.

[0042] According to the above-mentioned mass proportions, the Sophora japonica seed extract, Cistanche deserticola extract, Auricularia auricula powder, dried shrimp powder, dried plum powder, spinach powder, Amaranth powder and vitamin D are uniformly mixed to obtain the Sophora japonica seed and Cistanche deserticola composition for improving bone and joint health.

[0043] Example 4

[0044] A Sophora japonica and Cistanche deserticola composition for improving bone and joint health, comprising 4 parts of Sophora japonica extract, 4 parts of Cistanche deserticola extract, 5 parts of fungus powder, 3 parts of dried shrimp powder, 3 parts of dried plum powder, 3 parts of spinach powder, 3 parts of amaranth powder and 0.1 part of vitamin D in parts by weight.

[0045] The preparation method of the sophora japonica extract is as follows: crush the sophora japonica seeds with a wall-breaking machine, sieve through a 60-mesh sieve to obtain sophora japonica powder, evenly disperse the sophora japonica powder in a Na2HPO4·12H2O-C6H8O7·H2O buffer solution with a pH of 5.0 and a mass of 20 times that of the sophora japonica powder, add 0.5% of cellulase by mass of the sophora japonica powder, enzymolyze at 55°C for 2h, inactivate the enzyme at 80°C for 20min, add NaHCO3 to adjust the pH to 6.5 after cooling, add 2% of papain by mass of the sophora japonica powder, enzymolyze at 55°C for 1h, add 20% of γ-cyclodextrin by mass of the sophora japonica powder, embed at 65°C for 0.5h, inactivate the enzyme at 85°C for 30min, filter while hot, and spray-dry the filtrate to obtain the sophora japonica extract.

[0046] The preparation method of the Cistanche deserticola extract is as follows: drying the desert type Cistanche deserticola at 55°C for 8h, cutting into 1cm thin slices and powdering, sieving through a 60-mesh sieve to obtain the Cistanche deserticola powder, uniformly dispersing the Cistanche deserticola powder in a Na2HPO4·12H2O-C6H8O7·H2O buffer solution with a pH value of 5.0 and a mass of 20 times that of the Cistanche deserticola powder, adding 0.5% of cellulase by mass of the Cistanche deserticola powder, enzymolysis at 55°C for 2h, inactivating the enzyme at 85°C for 10min, adding NaHCO3 to adjust the pH value to 6.5 after cooling, adding 4% of glycoside hydrolase by mass of the Cistanche deserticola powder, enzymolysis at 50°C for 16h, inactivating the enzyme at 80°C for 30min, filtering after cooling, and spray drying the filtrate to obtain the Cistanche deserticola extract.

[0047] According to the above-mentioned mass proportions, the Sophora japonica seed extract, Cistanche deserticola extract, Auricularia auricula powder, dried shrimp powder, dried plum powder, spinach powder, Amaranth powder and vitamin D are uniformly mixed to obtain the Sophora japonica seed and Cistanche deserticola composition for improving bone and joint health.

[0048] Example 5

[0049] A Sophora japonica and Cistanche deserticola composition for improving bone and joint health, comprising 5 parts of Sophora japonica extract, 4 parts of Cistanche deserticola extract, 1 part of Auricularia auricula powder, 1 part of dried shrimp powder, 2 parts of dried plum powder, 3 parts of spinach powder, 3 parts of amaranth powder and 0.01 part of vitamin D in parts by weight.

[0050] The preparation method of the sophora japonica extract is as follows: crush the sophora japonica seeds with a wall-breaking machine, sieve through 80 meshes to obtain sophora japonica powder, evenly disperse the sophora japonica powder in a Na2HPO4·12H2O-C6H8O7·H2O buffer solution with a pH of 5.0 and a mass of 16 times that of the sophora japonica powder, add 1% of cellulase by mass of the sophora japonica powder, enzymolysis at 45°C for 1.5h, inactivate the enzyme at 90°C for 20min, add NaHCO3 to adjust the pH to 6.5 after cooling, add 0.5% of papain by mass of the sophora japonica powder, enzymolysis at 55°C for 2h, add 10% of α-cyclodextrin by mass of the sophora japonica powder, embed at 65°C for 1h, inactivate the enzyme at 85°C for 30min, filter while hot, spray-dry the filtrate to obtain the sophora japonica extract.

[0051] The preparation method of the Cistanche deserticola extract is as follows: drying the desert type Cistanche deserticola at 60°C for 8h, cutting into 0.6cm slices and powdering, sieving through an 80-mesh sieve to obtain the Cistanche deserticola powder, uniformly dispersing the Cistanche deserticola powder in a Na2HPO4·12H2O-C6H8O7·H2O buffer solution with a pH value of 5.0 and a mass of 16 times that of the Cistanche deserticola powder, adding 0.5% of cellulase by mass of the Cistanche deserticola powder, enzymolysis at 45°C for 1.5h, inactivating the enzyme at 90°C for 20min, adding NaHCO3 to adjust the pH value to 6.5 after cooling, adding 1% of glycoside hydrolase by mass of the Cistanche deserticola powder, enzymolysis at 40°C for 18h, inactivating the enzyme at 85°C for 30min, filtering after cooling, and spray drying the filtrate to obtain the Cistanche deserticola extract.

[0052] According to the above-mentioned mass proportions, the Sophora japonica seed extract, Cistanche deserticola extract, Auricularia auricula powder, dried shrimp powder, dried plum powder, spinach powder, Amaranth powder and vitamin D are uniformly mixed to obtain the Sophora japonica seed and Cistanche deserticola composition for improving bone and joint health.

[0053] Example 6

[0054] A Sophora japonica and Cistanche deserticola composition for improving bone and joint health, comprising 3 parts of Sophora japonica extract, 3 parts of Cistanche deserticola extract, 2 parts of fungus powder, 2 parts of dried shrimp powder, 2 parts of dried plum powder, 2 parts of spinach powder, 2 parts of amaranth powder and 0.05 parts of vitamin D in parts by weight.

[0055] The preparation method of the sophora japonica extract is as follows: crush the sophora japonica seeds with a wall-breaking machine, sieve through a 60-mesh sieve to obtain sophora japonica powder, evenly disperse the sophora japonica powder in a Na2HPO4·12H2O-C6H8O7·H2O buffer solution with a pH of 5.0 and a mass of 12 times that of the sophora japonica powder, add 1% of cellulase by mass of the sophora japonica powder, enzymolysis at 50°C for 1.5 hours, inactivate the enzyme at 90°C for 10 minutes, add NaHCO3 to adjust the pH to 6.5 after cooling, add 1.5% of papain by mass of the sophora japonica powder, enzymolysis at 60°C for 1.5 hours, add 40% of α-cyclodextrin by mass of the sophora japonica powder, embed at 65°C for 1 hour, inactivate the enzyme at 85°C for 30 minutes, filter while hot, and spray-dry the filtrate to obtain the sophora japonica extract.

[0056] The preparation method of the Cistanche deserticola extract is as follows: drying the desert type Cistanche deserticola at 55°C for 12h, cutting into 0.3cm thin slices and powdering, sieving through a 60-mesh sieve to obtain the Cistanche deserticola powder, uniformly dispersing the Cistanche deserticola powder in a Na2HPO4·12H2O-C6H8O7·H2O buffer solution with a pH value of 5.0 and a mass of 12 times that of the Cistanche deserticola powder, adding 1.5% of cellulase by mass of the Cistanche deserticola powder, enzymolysis at 65°C for 1.5h, inactivating the enzyme at 90°C for 10min, adding NaHCO3 to adjust the pH value to 6.5 after cooling, adding glycoside hydrolase at a mass of 2.5% of the Cistanche deserticola powder, enzymolysis at 50°C for 14h, inactivating the enzyme at 90°C for 15min, filtering after cooling, and spray drying the filtrate to obtain the Cistanche deserticola extract.

[0057] According to the above-mentioned mass proportions, the Sophora japonica seed extract, Cistanche deserticola extract, Auricularia auricula powder, dried shrimp powder, dried plum powder, spinach powder, Amaranth powder and vitamin D are uniformly mixed to obtain the Sophora japonica seed and Cistanche deserticola composition for improving bone and joint health.

[0058] Comparative Example 1

[0059] In the composition, the Sophora japonica extract is replaced by Sophora japonica powder, the Cistanche deserticola extract is replaced by Cistanche deserticola powder, and other parameters are the same as those in Example 1.

[0060] Comparative Example 2

[0061] In the composition, in the preparation method of the Sophora japonica flower extract, no cyclodextrin is added for embedding, and other parameters are the same as those in Example 1.

[0062] Comparative Example 3

[0063] In the composition, no Sophora japonica extract was added among the components, and other parameters were the same as those in Example 1.

[0064] Comparative Example 4

[0065] In the composition, no Cistanche deserticola extract was added among the components, and other parameters were the same as those in Example 1.

[0066] Effect verification

[0067] (1) Experimental animals

[0068] Six-week-old female mice (C57BL / 6, purchased from Guangdong Medical Experimental Animal Center).

[0069] (2) Model establishment

[0070] Before modeling, mice were fasted and deprived of water for 12 hours. Model group: After anesthesia, the back of the mice was depilated, and a longitudinal incision of about 1 cm was made above the left and right hip joints. The fallopian tubes were ligated at the upper and lower parts of the uterine horns, and then both ovaries were removed. Finally, the wounds were sutured to establish a postmenopausal osteoporosis model. Sham operation group: The treatment method was the same as the model group, but only the fat near the ovaries was removed.

[0071] (3) Experimental groups

[0072] Examples 1-6 and Comparative Examples 1-4 were divided into 10 groups, and a sham operation group and a model group were further provided.

[0073] (4) Experimental plan

[0074] All mice were acclimatized for one week, with free access to food and water, and a light / dark cycle of 12h. After one week, the mice were randomly divided into 10 groups, namely, a sham operation group, a model group, embodiments 1 to 6, and comparative examples 1 to 4, with 10 mice in each group. Both the sham operation group and the model group were fed with normal feed, and the embodiment group and the comparative example group were fed with feed mixed with 10% of the corresponding composition. After 2 weeks of feeding, the mice were anesthetized, the sham operation group removed fat near the ovaries, and the remaining groups established a postmenopausal osteoporosis model. Continue to feed according to the preoperative feeding method. After 10 weeks of the experiment, the mice were anesthetized after fasting for 8 hours and were killed. Serum and femur were collected. Histopathological analysis was performed.

[0075] (5) Testing indicators

[0076] Bone density and bone microstructure testing

[0077] Femoral samples were scanned using Micro-CT scanner software, and trabecular microarchitecture parameters including bone mineral density (BMD), bone volume fraction (BV / TV), trabecular number (Tb.N), trabecular separation (Tb.Sp), trabecular thickness (Tb.Th), and cortical area fraction (Ct.Ar / Tt.Ar) were obtained by three-dimensional tissue morphometric analysis.

[0078] Bone metabolism markers

[0079] The PINP level in the serum of mice in each group was determined using a PINP enzyme-linked immunosorbent assay kit.

[0080] (6) Results statistics and analysis

[0081] The results were expressed as mean ± standard deviation. Each index was statistically analyzed using SPSS 16.0 statistical software.

[0082] (7) Results

[0083] 7.1 Comparison of bone density values ​​among mice in each group

[0084] Table 1 Comparison of femoral bone density values ​​of mice in each group

[0085]

[0086] Bone density value is an important indicator of bone quality, reflecting the degree of osteoporosis and an important basis for predicting the risk of fracture. The smaller the value, the more serious the osteoporosis. As can be seen from the results in Table 1, compared with the sham operation group, the bone density value of the model group decreased significantly. Compared with the model group, the bone density values ​​of the embodiment 1 to 6 groups and the comparative example 1 to 4 groups all increased, but the effect of the comparative example 1 to 2 groups was obviously not as good as that of the embodiment 1 to 6 groups, indicating that the raw materials that have not been extracted or embedded are slightly less effective; in addition, the effect of the comparative example 3 to 4 groups is also not as good as that of the embodiment 1 to 6 groups, indicating that the effect of adding the sophora japonica powder extract and the Cistanche deserticola extract alone is not as good as the composite combination of the two.

[0087] 7.2 Comparison of trabecular bone microstructure parameters among mice groups

[0088] Table 2 Comparison of trabecular bone microstructure parameters in each group of mice

[0089]

[0090] Indicators such as bone volume fraction (BV / TV), trabecular number (Tb.N), trabecular separation (Tb.Sp), trabecular thickness (Tb.Th), and cortical area fraction (Ct.Ar / Tt.Ar) reflect the fine structure of bone tissue, among which the larger the value of trabecular separation (Tb.Sp), the more severe the osteoporosis; the smaller the values ​​of the other indicators, the more severe the osteoporosis. From the results in Table 2, it can be seen that compared with the sham operation group, the trabecular bone mass per tissue volume, the number of trabeculae and the thickness of trabeculae in the model group were significantly reduced, and the trabecular spacing was significantly increased. Compared with the model group, the trabecular bone mass per tissue volume, the number of trabeculae and the thickness of trabeculae in the embodiment 1 to 6 groups and the comparative example 1 to 4 groups were increased, and the trabecular spacing was decreased. However, the effects of the comparative examples 1 to 2 groups were obviously not as good as those of the examples 1 to 6 groups, indicating that the raw materials that had not been extracted or embedded had slightly worse effects; in addition, the effects of the comparative examples 3 to 4 groups were also not as good as those of the examples 1 to 6 groups, indicating that the effects of adding the Sophora japonica flour extract and the Cistanche deserticola extract alone were not as good as the combined effect of the two.

[0091] 7.3 Comparison of P1NP levels in serum of mice in each group

[0092] Table 3 Comparison of P1NP levels in serum of mice in each group

[0093]

[0094] P1NP refers to the amino-terminal extension peptide of type 1 procollagen, which is one of the direct and important indicators of bone metabolism. It can monitor osteoblast activity and bone formation and is often used to monitor osteoporosis patients. From the results in Table 3, it can be seen that compared with the sham operation group, the P1NP level in the model group decreased significantly. Compared with the model group, the P1NP levels in Example 1 to 6 and Comparative Example 1 to 4 groups increased significantly, but the effect of Comparative Example 1 to 4 groups was obviously not as good as that of Example 1 to 6 groups, indicating that the raw materials that have not been extracted or embedded have a slightly worse effect; in addition, the effect of Comparative Example 3 to 4 groups is also not as good as that of Example 1 to 6 groups, indicating that the effect of adding Sophora japonica powder extract and Cistanche deserticola extract alone is not as good as the composite combination of the two.

[0095] From the above experimental results, it can be seen that the composition of the present invention has a good effect on improving bone and joint health, can effectively improve the bone loss caused by ovariectomy in mice, and improve the activity of osteoblasts.

[0096] By comparing the data of Examples 1 to 6 and Comparative Examples 1 to 4, it can be seen that the effect of the composition of Sophora japonica and Cistanche deserticola on improving bone and joint health is far less than that of the composition of Sophora japonica extract without enzymatic extraction; and the effect of Sophora japonica extract without cyclodextrin encapsulation treatment is also slightly worse; similarly, the effect of adding Sophora japonica powder extract and Cistanche deserticola extract alone is not as good as the combined combination of the two.

[0097] The above is a detailed description of the embodiments of the present invention. The implementation methods of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations and simplifications made without departing from the essence and principles of the present invention are equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A composition of Sophora japonica and Cistanche deserticola for improving bone and joint health, characterized in that: The composition is composed of the following components in parts by weight: 3 to 6 parts of Sophora japonica extract, 3 to 6 parts of Cistanche deserticola extract, 1 to 5 parts of Fungus powder, 1 to 3 parts of dried shrimp powder, 1 to 3 parts of dried plum powder, 1 to 3 parts of spinach powder, 1 to 3 parts of amaranth powder and 0.01 to 0.1 parts of vitamin D; The preparation method of the sophora japonica extract comprises: uniformly dispersing sophora japonica powder in a Na2HPO4·12H2O-C6H8O7·H2O buffer, adding cellulase for enzymolysis, inactivating the enzyme, adjusting the pH value after cooling, then adding papain for enzymolysis, adding cyclodextrin for embedding, inactivating the enzyme, filtering, and spray-drying the filtrate to obtain the sophora japonica extract; The particle size of the sophora japonica flour is 40-80 mesh; The pH of the Na2HPO4·12H2O-C6H8O7·H2O buffer solution is between 4.8 and 5.2, and the mass ratio is 1:10-20 for the sophora japonica rice flour and the buffer solution; The amount of cellulase added is 0.2% to 2% of the mass of the sophora japonica rice flour; the temperature for enzymolysis of the cellulase is 45°C to 55°C, and the enzymolysis time is 1.5h to 2.5h; the temperature for inactivating the enzyme is 80°C to 90°C, and the time is 10min to 30min; the pH value is adjusted to a range of 6.0 to 7.0; The amount of papain added is 0.2% to 2% of the mass of the sophora japonica flour, the enzymolysis temperature of the papain is 55° C. to 65° C., and the enzymolysis time is 1 h to 2 h; the cyclodextrin is one of α-cyclodextrin, β-cyclodextrin, and γ-cyclodextrin, the amount of which is 10% to 50% of the mass of the sophora japonica flour, the embedding temperature is 55° C. to 65° C., and the embedding time is 0.5 h to 1 h; the enzyme inactivation temperature is 80° C. to 90° C., and the time is 15 min to 30 min; The preparation method of the Cistanche deserticola extract comprises: uniformly dispersing the Cistanche deserticola powder in a Na2HPO4·12H2O-C6H8O7·H2O buffer, adding cellulase for enzymolysis, inactivating the enzyme, adjusting the pH value after cooling, adding glycoside hydrolase for enzymolysis, inactivating the enzyme, filtering, and spray-drying the filtrate to obtain the Cistanche deserticola extract; In the preparation method of the Cistanche deserticola extract, the particle size of the Cistanche deserticola powder is 40-80 mesh; The pH of the Na2HPO4·12H2O-C6H8O7·H2O buffer solution is between 4.8 and 5.2, and the mass ratio is Cistanche deserticola powder: buffer solution = 1:10-20; The amount of cellulase added is 0.5% to 3% of the mass of Cistanche deserticola powder, the temperature of cellulase hydrolysis is 45°C to 65°C, and the time is 1h to 2h; the temperature of cellulase inactivation is 80°C to 90°C, and the time is 10min to 30min; The pH value is adjusted to a range of 6.0 to 7.0; The addition amount of the glycoside hydrolase is 1% to 5% of the mass of the Cistanche powder, the temperature of the glycoside hydrolase enzymolysis is 40°C to 50°C, and the time is 12h to 18h; the temperature of the glycoside hydrolase inactivation is 80°C to 90°C, and the time is 15min to 30min.

2. The composition according to claim 1, characterized in that The sophora japonica flour is obtained by breaking the wall, pulverizing and sieving.

3. The composition according to claim 1, characterized in that The Cistanche deserticola extract is a desert type Cistanche deserticola extract.

4. The composition according to claim 1, characterized in that The Cistanche deserticola powder is obtained by drying, slicing, powdering and sieving the Cistanche deserticola, the drying temperature is 45° C. to 60° C., the time is 6 h to 12 h, and the slice thickness is 0.1 cm to 1 cm.

5. The method for preparing the composition of Sophora japonicae and Cistanche deserticola for improving bone and joint health according to any one of claims 1 to 4, characterized in that: The following steps are involved: The sophora japonica extract, the Cistanche deserticola extract, the auricularia auricula powder, the dried shrimp powder, the dried plum powder, the spinach powder, the amaranth powder and the vitamin D are uniformly mixed according to weight proportions to obtain a composition.

6. A product for improving bone and joint health, comprising the Sophora japonica and Cistanche deserticola composition according to any one of claims 1 to 4, wherein the product is a medicine or a health product.

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