Moisturizing and skin-moistening composition containing recombinant collagen and application of moisturizing and skin-moistening composition

Abstract

The invention relates to a moisturizing and skin-moistening composition containing recombinant collagen and application thereof. The moisturizing and skin-moistening composition containing the recombinant collagen comprises the recombinant collagen, an emblic leafflower fruit extract, a common bombax flower extract, a finger orange extract and serine. The invention creatively finds that the composition composed of the recombinant collagen, the emblic leafflower fruit extract, the common bombax flower extract, the finger orange extract and the serine has a remarkable moisturizing effect and can deeply nourish, effectively lock moisture, relieve dryness and keep the skin in a moisturized state for a long time. And the five components have a certain synergistic effect, can go deep into the bottom layer of the skin, provide all-around moistening for the skin, and are mild, non-irritant and good in stability.

Description

Technical Field

[0001] The invention belongs to the technical field of skin care products and relates to a moisturizing and emollient composition containing recombinant collagen and application thereof. Background Art

[0002] The skin is the largest organ in the human body and plays an important role in protecting and regulating body temperature. However, the skin often faces the challenge of water loss. In modern life, many factors can accelerate the loss of skin water, such as prolonged exposure to air-conditioned environments, dry indoor air, frequent cleaning, use of irritating cosmetics, ultraviolet radiation in the external environment, air pollution, etc. Water loss from the skin can lead to dry, flaky, and rough skin, and then wrinkles and other signs of aging. Therefore, effective moisturizing of the skin has become a key requirement for skin care.

[0003] Collagen is an important component of the skin. It forms a fibrous support structure in the skin, providing elasticity and firmness to the skin. As we age, the body's own collagen synthesis gradually decreases, and the collagen content in the skin also decreases, which is one of the important reasons for skin sagging and aging. Due to the large molecular weight and other reasons, the skin has limited absorption efficiency of natural collagen. Recombinant collagen is collagen produced by genetic engineering technology. Compared with traditional natural collagen, recombinant collagen has the advantages of high purity, good biological activity and low immunogenicity. In the field of skin care, recombinant collagen can be better absorbed and utilized by the skin, replenish the collagen lost by the skin, improve the structure and function of the skin from the inside, and also has a good moisturizing effect.

[0004] Therefore, developing a moisturizing and moisturizing composition containing recombinant collagen can meet people's growing demand for skin health and beauty, and has broad market prospects. Summary of the invention

[0005] In view of the deficiencies of the prior art, the object of the present invention is to provide a moisturizing and skin-moisturizing composition containing recombinant collagen and its application.

[0006] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a moisturizing and skin-moisturizing composition containing recombinant collagen, wherein the moisturizing and skin-moisturizing composition containing recombinant collagen comprises recombinant collagen, emblica fruit extract, kapok flower extract, finger orange extract and serine.

[0008] Among them, recombinant collagen contains a large number of hydrophilic genes, has good film-forming properties, and maintains moisture in the stratum corneum of the skin. At the same time, its tropism can guide epithelial cells to quickly enter the damaged area, effectively increase the speed of skin regeneration, shorten the wound healing time, and thus restore the skin barrier function.

[0009] Phyllanthus emblica extract is rich in vitamin C and polyphenols, which can effectively inhibit the production of free radicals and reduce oxidative damage. The small molecule tannic acid in Phyllanthus emblica can inhibit the activity of tyrosinase and achieve the effect of whitening and removing spots. Phyllanthus emblica also has soothing and anti-inflammatory effects, reduces skin inflammation, relieves allergic symptoms, and repairs damaged skin barriers, which is especially suitable for sensitive skin. Phyllanthus emblica can promote the synthesis of natural moisturizing factors in the skin, enhance the skin's barrier function, and maintain skin moisture.

[0010] Kapok flowers mainly contain flavonoids, organic acids, steroids, triterpenes, coumarins and other chemical ingredients, which can effectively inhibit the production of melanin and achieve a whitening effect. At the same time, it has a strong antioxidant ability, can resist aging, soothe the skin, regulate oil secretion, reduce acne, and bring comprehensive nourishment and protection to the skin.

[0011] Finger lime is rich in fruit acid, bioflavonoids, polyphenols, natural glucose and other ingredients, and is known as "plant caviar". Finger lime extract can accelerate the metabolism of skin keratin cells, avoid the accumulation of keratin and cause dull skin, and mainly achieve the effect of keratin metabolism with a low pH value, presenting a smooth, bright and even skin tone. Finger lime extract has the effect of smoothing and brightening the skin, promoting gentle skin metabolism, preventing dullness and even skin tone.

[0012] Serine Serine has good hydrophilicity and has multiple functions such as moisturizing, promoting collagen production, anti-oxidation, repairing damaged skin and regulating skin pH. It can effectively improve skin condition and delay aging.

[0013] The present invention creatively finds that the composition of recombinant collagen, emblica extract, kapok extract, finger lime extract and serine has significant moisturizing effect, can deeply nourish, effectively lock moisture, relieve dryness, and keep the skin moisturized for a long time. And the five ingredients have a certain synergistic effect, can penetrate deep into the bottom layer of the skin, provide all-round moisturizing for the skin, are mild and non-irritating, and have good stability.

[0014] Preferably, the nucleotide sequence encoding the recombinant collagen is the sequence shown in SEQ ID NO:1.

[0015] Compared with recombinant collagen from other sources, the present invention also finds that the recombinant collagen from the above specific source is better in moisturizing and skin care when combined with emblica emblica extract, kapok flower extract, finger orange extract and serine.

[0016] Preferably, the moisturizing and moisturizing composition containing recombinant collagen comprises, by weight, 4-8 parts of recombinant collagen, 4-8 parts of emblica extract, 4-12 parts of kapok extract, 2-6 parts of finger lime extract and 10-18 parts of serine.

[0017] Furthermore, when the above five components are combined in a specific mass ratio, they have more significant effects in moisturizing and moisturizing the skin.

[0018] The weight proportions of the recombinant collagen can be 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, etc.

[0019] The weight proportions of the Emblica officinalis extract may be 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, etc.

[0020] The weight proportions of the kapok flower extract may be 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, etc.

[0021] The weight proportions of the finger orange extract may be 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, etc.

[0022] The weight proportions of the serine may be 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, etc.

[0023] Any other specific point value within the above numerical range can be selected, and will not be described in detail here.

[0024] In a second aspect, the present invention provides use of the moisturizing and moisturizing composition containing recombinant collagen according to the first aspect in the preparation of cosmetics.

[0025] Preferably, the cosmetics include lotion, emulsion, cream, mask, essence or gel.

[0026] Preferably, the mass percentage of the moisturizing and moisturizing composition containing recombinant collagen in the cosmetic is 15-25%, for example 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, etc. Other specific point values ​​within this numerical range can be selected and will not be described here one by one.

[0027] In a third aspect, the present invention provides a lotion containing recombinant collagen, comprising, by weight: 120-160 parts of water, 3-11 parts of moisturizing agent and 24-52 parts of the moisturizing and emollient composition containing recombinant collagen according to the first aspect.

[0028] The weight proportions of the water may be 120 parts, 125 parts, 130 parts, 135 parts, 140 parts, 145 parts, 150 parts, 155 parts, 160 parts, etc.

[0029] The weight proportions of the moisturizing agent may be 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, etc.

[0030] The weight proportions of the moisturizing and moisturizing composition containing recombinant collagen can be 24 parts, 26 parts, 28 parts, 30 parts, 34 parts, 38 parts, 42 parts, 46 parts, 50 parts, 52 parts, etc.

[0031] Any other specific point value within the above numerical range can be selected, and will not be described in detail here.

[0032] Preferably, the dosage form of the lotion includes a spray.

[0033] Preferably, the moisturizing agent includes any one or a combination of at least two of glycerin, propylene glycol, panthenol, fructose, butylene glycol, 1,2-hexanediol, pullulan, tamarind seed polysaccharide and squalane.

[0034] Preferably, the moisturizing agent comprises a combination of pullulan, tamarind seed polysaccharide and squalane.

[0035] The present invention also creatively discovered that when a combination of pullulan, tamarind seed polysaccharide and squalane is selected as a moisturizer, the three can play a synergistic role in moisturizing effect. At the same time, this combination and the moisturizing and lotion combination containing recombinant collagen described in the first aspect can also achieve a synergistic effect in moisturizing effect.

[0036] Preferably, the lotion containing recombinant collagen also includes 1-3 parts of preservatives, such as 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, etc. Any other specific point value within this numerical range can be selected and will not be described here one by one.

[0037] Preferably, the preservative includes any one or a combination of at least two of phenoxyethanol, benzyl alcohol, propylparaben, butylparaben, potassium sorbate, chlorphenesin, sodium benzoate, caprylhydroxamic acid or levulinic acid.

[0038] In a fourth aspect, the present invention provides a method for preparing the lotion containing recombinant collagen according to the third aspect, the preparation method comprising:

[0039] The moisturizing and emollient composition containing recombinant collagen described in the first aspect, a moisturizer and water are mixed, stirred evenly, and filtered to obtain the lotion containing recombinant collagen.

[0040] Compared with the prior art, the present invention has the following beneficial effects:

[0041] The present invention creatively finds that the composition of recombinant collagen, emblica extract, kapok extract, finger lime extract and serine has significant moisturizing effect, can deeply nourish, effectively lock moisture, relieve dryness, and keep the skin moisturized for a long time. And the five ingredients have a certain synergistic effect, can penetrate deep into the bottom layer of the skin, provide all-round moisturizing for the skin, are mild and non-irritating, and have good stability. DETAILED DESCRIPTION

[0042] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0043] In the following examples, unless otherwise specified, the reagents and consumables used were purchased from conventional reagent manufacturers in the field; unless otherwise specified, the experimental methods and technical means used were conventional methods and means in the field.

[0044] The weight percentages of the pullulan and tamarind polysaccharide mentioned below are all calculated based on the actual effective ingredient content contained in the commercially available raw materials. The commercially available raw materials may also selectively contain any one or a combination of at least two of solvents, fillers, diluents, stabilizers, pH adjusters, antibacterial agents, antioxidants or impurities within the permitted range.

[0045] The pullulan involved in the following examples was purchased from Shanghai MacLean Biochemical Technology Co., Ltd., product number P816954;

[0046] Tamarind seed polysaccharide was purchased from Guangzhou Aoxue Chemical Co., Ltd., with the product number being XILOGEL HS-Tamarind seed polysaccharide-9070130.

[0047] The emblica extract, kapok extract and finger orange extract involved in the following examples are prepared by the following method:

[0048] The commercially available raw materials of emblica, kapok and finger orange were crushed into 40 meshes respectively, 8 times the weight of water was added, and the products were extracted at 80°C for 2 hours. The supernatant was filtered to obtain emblica, kapok and finger orange extracts respectively. The extracts were concentrated and dried to obtain emblica extract, kapok extract and finger orange extract respectively.

[0049] Preparation Example 1

[0050] This preparation example provides a recombinant collagen freeze-dried powder, and its preparation method refers to ZL 202310123476.3

[0051] The method disclosed in Preparation Example 1 is carried out as follows:

[0052] (1) Construction of recombinant plasmid: According to the nucleotide sequence shown in SEQ ID NO: 1, the pET28a(+) vector suitable for the expression vector Escherichia coli host was selected and sent to a sequencing company for synthesis to obtain the recombinant collagen expression vector pET-28a-1.

[0053] (2) Construction of recombinant fermentation strain: The pET-28a-1 expression vector was transferred into competent Escherichia coli (BL21 (DE3)) cells by chemical transformation, and the positive clones in the transformed plate colonies were screened by agarose gel electrophoresis. The screened positive clones were transferred to 2 mL LB medium and cultured at 37°C, 200 rpm for 12 h. The cultured bacterial solution was diluted 1-fold with 50% glycerol by volume, dispensed into cryovials, and frozen at -80°C to obtain a recombinant collagen fermentation strain.

[0054] (3) Fermentation culture of recombinant fermentation strains: 10 μL of recombinant collagen fermentation broth was spread on a solid LB plate, cultured in a 37°C incubator for 12 h, and then a single clone was selected and inoculated into 50 mL of LB liquid culture medium containing 50 μg / mL kanamycin. The culture was placed in a shaker at 37°C, 200 rpm for 14 h, and then inoculated into 200 mL of LB liquid culture medium containing 50 μg / mL kanamycin at a ratio of 1:100. The culture was cultured at 37°C until the OD of the bacterial solution was 0.6, and then IPTG was added to a final concentration of 1 mM. The culture was continued for 4 h, and then the bacteria were collected by centrifugation at 4°C, 4000 rpm for 15 min.

[0055] (4) Purification of recombinant collagen: The bacteria were ultrasonically disrupted and centrifuged. The precipitate was washed with TritonX-100 washing solution and centrifuged again to remove the supernatant. 6M guanidine hydrochloride was added to the precipitate and the pH was adjusted to 8. After the precipitate was fully dissolved, the solution was passed through a nickel affinity chromatography resin and eluted in sequence using 20mM, 50mM, 100mM, 200mM, and 500mM concentration gradient imidazole solutions. The eluate was concentrated using a protein concentration column, and the concentrate was collected and dialyzed overnight (dialysis bag, RC membrane, 14KD, flat width 4mm (Shenggong F600112)). The protein concentration was then detected using a BCA kit, and 0.5mL of a 5% volume concentration mannitol aqueous solution was added to every 5mg of protein and mixed with the mannitol aqueous solution. The mixture was then pre-frozen at -40°C for 10min using a freeze dryer and finally freeze-dried at -35°C to obtain a recombinant collagen freeze-dried powder.

[0056] Example 1

[0057] The present embodiment provides a moisturizing and skin-moisturizing composition containing recombinant collagen, which comprises the following effective ingredients in parts by weight: 6 parts of recombinant collagen, 6 parts of emblica extract, 8 parts of kapok extract, 4 parts of finger orange extract, and 14 parts of serine.

[0058] Example 2

[0059] The present embodiment provides a moisturizing and skin-moisturizing composition containing recombinant collagen, which comprises the following effective ingredients in parts by weight: 4 parts of recombinant collagen, 8 parts of emblica extract, 4 parts of kapok extract, 6 parts of finger orange extract, and 10 parts of serine.

[0060] Example 3

[0061] The present embodiment provides a moisturizing and skin-moisturizing composition containing recombinant collagen, which comprises the following effective ingredients in parts by weight: 8 parts of recombinant collagen, 4 parts of emblica extract, 12 parts of kapok extract, 2 parts of finger orange extract, and 18 parts of serine.

[0062] Example 4

[0063] This embodiment provides a moisturizing and skin-moisturizing composition containing recombinant collagen, which differs from Embodiment 1 only in that the recombinant collagen is replaced by type III recombinant collagen (purchased from Guangzhou Zhiyan Chemical Co., Ltd.).

[0064] Example 5

[0065] The present embodiment provides a moisturizing and skin-moisturizing composition containing recombinant collagen, which comprises the following effective ingredients in parts by weight: 6 parts of recombinant collagen, 2 parts of emblica extract, 15 parts of kapok extract, 9 parts of finger orange extract, and 6 parts of serine.

[0066] Example 6

[0067] The present embodiment provides a moisturizing and skin-moisturizing composition containing recombinant collagen, which comprises the following effective ingredients in parts by weight: 6 parts of recombinant collagen, 13 parts of emblica fruit extract, 2 parts of kapok flower extract, 10 parts of finger orange extract, and 7 parts of serine.

[0068] Comparative Example 1

[0069] This comparative example provides a moisturizing and skin-moisturizing composition containing recombinant collagen, which comprises the following effective ingredients in parts by weight: 6 parts of recombinant collagen, 6 parts of emblica fruit extract, 8 parts of kapok flower extract, and 4 parts of finger orange extract.

[0070] Comparative Example 2

[0071] This comparative example provides a moisturizing and skin-moisturizing composition containing recombinant collagen, which comprises the following effective ingredients in parts by weight: 6 parts of recombinant collagen, 6 parts of emblica fruit extract, 8 parts of kapok flower extract, and 14 parts of serine.

[0072] Comparative Example 3

[0073] This comparative example provides a moisturizing and skin-moisturizing composition containing recombinant collagen, which comprises the following effective ingredients in parts by weight: 6 parts of recombinant collagen, 6 parts of emblica fruit extract, 4 parts of finger orange extract, and 14 parts of serine.

[0074] Comparative Example 4

[0075] This comparative example provides a moisturizing and skin-moisturizing composition containing recombinant collagen, which comprises the following effective ingredients in parts by weight: 6 parts of recombinant collagen, 8 parts of kapok extract, 4 parts of finger orange extract, and 14 parts of serine.

[0076] Comparative Example 5

[0077] This comparative example provides a moisturizing and moisturizing composition containing recombinant collagen, which includes the following effective ingredients in parts by weight: 6 parts of emblica extract, 8 parts of kapok extract, 4 parts of finger orange extract, and 14 parts of serine.

[0078] Application Example 1

[0079] This application example provides a moisturizing spray containing recombinant collagen, and its preparation raw materials include, by weight, 140 parts of water, 2 parts of pullulan, 2 parts of tamarind seed polysaccharide, 3 parts of squalane, and 38 parts of the moisturizing and moisturizing composition of Example 1.

[0080] The preparation method is as follows: water, pullulan, tamarind seed polysaccharide, squalane and the moisturizing and emollient composition of Example 1 are mixed, stirred evenly, filtered, and filled to obtain a moisturizing spray containing recombinant collagen.

[0081] Application Example 2

[0082] This application example provides a moisturizing spray containing recombinant collagen, and its preparation raw materials include, by weight, 120 parts of water, 1 part of pullulan, 1 part of tamarind polysaccharide, 1 part of squalane, and 29 parts of the moisturizing and emollient composition of Example 2. The preparation method thereof refers to Application Example 1.

[0083] Application Example 3

[0084] This application example provides a moisturizing spray containing recombinant collagen, and its raw materials include, by weight, 160 parts of water, 4 parts of pullulan, 4 parts of tamarind polysaccharide, 3 parts of squalane, and 43 parts of the moisturizing and emollient composition of Example 3. The preparation method thereof refers to Application Example 1.

[0085] Application Examples 4-6

[0086] This application example provides three moisturizing sprays containing recombinant collagen, which differ from application example 1 only in that the moisturizing and skin-moisturizing composition of example 1 is replaced by the moisturizing and skin-moisturizing compositions of examples 4 to 6 in equal amounts. The preparation method thereof refers to application example 1.

[0087] Application Example 7

[0088] This application example provides a moisturizing spray containing recombinant collagen, and its raw materials include 140 parts of water, 3.5 parts of pullulan, 3.5 parts of tamarind polysaccharide, and 38 parts of the moisturizing and emollient composition of Example 1. The preparation method thereof refers to Application Example 1.

[0089] Application Example 8

[0090] This application example provides a moisturizing spray containing recombinant collagen, and its preparation raw materials include, by weight, 140 parts of water, 2.8 parts of pullulan, 4.2 parts of squalane, and 38 parts of the moisturizing and emollient composition of Example 1. The preparation method thereof refers to Application Example 1.

[0091] Application Example 9

[0092] This application example provides a moisturizing spray containing recombinant collagen, and its raw materials include 140 parts of water, 2.8 parts of tamarind seed polysaccharide, 4.2 parts of squalane, and 38 parts of the moisturizing and emollient composition of Example 1 in terms of weight. The preparation method thereof refers to Application Example 1.

[0093] Application Example 10

[0094] This application example provides a moisturizing spray containing recombinant collagen, and its preparation raw materials include 140 parts of water and 45 parts of the moisturizing and moisturizing composition of Example 1 in parts by weight. The preparation method thereof refers to Application Example 1.

[0095] Comparative Application Examples 1-5

[0096] This comparative application example provides five moisturizing sprays containing recombinant collagen, which differ from application example 1 only in that the moisturizing and skin-moisturizing composition of example 1 is replaced by the moisturizing and skin-moisturizing compositions of comparative examples 1-5 in equal amounts.

[0097] Test Example 1

[0098] Skin safety test:

[0099] The products prepared in Application Examples 1-10 were placed in a patch tester, and the patch tester with the test substance added was attached to the flexor side of the forearm of the test persons (30 people), and the patch was lightly pressed with the palm to evenly apply it to the skin for 24 hours; the skin irritation and sensitization were observed according to Table 1 (Skin Reaction Grading Standard for Skin Closure Patch Test) 30 minutes, 24 hours, and 48 hours after the removal of the test substance patch tester, and the observation results were recorded.

[0100] Table 1

[0101]

[0102] The results showed that no adverse skin reactions occurred in the products of Application Examples 1-10, indicating that the moisturizing spray containing recombinant collagen involved in the present invention is safe and non-irritating to the skin.

[0103] Test Example 2

[0104] Moisturizing effect test:

[0105] 1) Test subjects: 150 female volunteers (aged 20-25) with no history of skin allergies, divided into 15 groups, 10 people in each group;

[0106] 2) Test area: inner arm skin, area 5cm×10cm;

[0107] 3) Test environment: The test environment is 21°C and 50% humidity;

[0108] 4) Testing instrument: Skin moisture content tester CM 825 (Courage & Khazaka, Germany).

[0109] 5) Test method: No products (cosmetics or topical drugs) can be used 3 days before the test, and no contact with water within 3 hours before the test. The volunteers cleaned their arms and dried them completely. The moisturizing sprays prepared in Application Examples 1-10 and Comparative Application Examples 1-5 were applied to the test areas of the volunteers in each group. The application method was uniform application. The skin moisture content of the test area was tested with a skin moisture content tester before the test (recorded as 0h), 1h after the trial, and 4h after the trial. The test results are shown in Table 2.

[0110] Table 2

[0111]

[0112]

[0113] According to the data in Table 2, the moisturizing and moisturizing composition containing recombinant collagen involved in the present invention can effectively increase the moisture content of the skin. After using the product of Application Example 1 for 1 hour, the moisture content of the skin is increased by 39.86% compared with before use; after using the product of Application Example 1 for 4 hours, the moisture content of the skin is increased by 35.89% compared with before use.

[0114] Comparing Application Example 1 with Application Example 4, it can be seen that the recombinant collagen from a specific source involved in the present invention, when combined with Phyllanthus emblica extract, Kapok extract, Citrus aurantium extract and serine, has a better moisturizing effect.

[0115] Comparing Application Example 1 with Application Examples 5-6, it can be seen that recombinant collagen, emblica extract, kapok extract, finger orange extract and serine can achieve better moisturizing and emollient effects at a specific ratio.

[0116] Comparing Application Example 1 with Application Examples 7-9, it can be seen that when pullulan, tamarind seed polysaccharide and squalane are selected as moisturizers, the three can achieve a synergistic effect in moisturizing and emollient.

[0117] By comparing Application Example 1 with Application Example 10, it can be seen that the moisturizing and emollient composition containing recombinant collagen and the specific moisturizer can achieve a synergistic effect in moisturizing.

[0118] By comparing Application Example 1 with Comparative Application Examples 1-5, it can be seen that the recombinant collagen, emblica extract, kapok extract, finger orange extract and serine in the moisturizing and moisturizing composition containing recombinant collagen of the present invention cooperate with each other and have a synergistic effect in moisturizing.

[0119] Test Example 3

[0120] Soothing effect test:

[0121] 1) Test subjects: 150 volunteers with dry skin and sensitive skin (aged between 18-40), divided into 15 groups, with 10 people in each group;

[0122] 2) Test area: inner arm skin, area 3cm×3cm;

[0123] 3) Test environment: The test environment is 21°C and 50% humidity;

[0124] 4) Testing instrument: C-Cube skin analyzer;

[0125] 5) Test method: Before the test, take a photo of the inner forearm of the volunteer and measure the skin erythema index (EI value). Apply a 1wt% capsaicin aqueous solution to the test area. After the skin becomes hot and swollen (recorded as 0min), record the data of various skin indicators. Apply 50mg / cm2 of the moisturizing spray prepared in Examples 1-10 and Comparative Examples 1-5 evenly to the test area. 2 , data of the test area were recorded 30min and 60min after applying the sample, and the results are shown in Table 3.

[0126] Table 3

[0127]

[0128]

[0129] According to the data in Table 3, the moisturizing and moisturizing composition containing recombinant collagen of the present invention has a good soothing effect. After using the product of Application Example 1 for 30 minutes, the skin erythema index decreased by 9.27%; after using the product of Application Example 1 for 60 minutes, the skin erythema index decreased by 15.04%.

[0130] Comparing Application Example 1 with Application Example 4, it can be seen that the recombinant collagen from a specific source involved in the present invention, when combined with Phyllanthus emblica extract, Kapok extract, Citrus aurantium extract and serine, can effectively improve the soothing effect of the composition.

[0131] Comparing Application Example 1 with Application Examples 5-6, it can be seen that recombinant collagen, emblica extract, kapok extract, finger orange extract and serine can achieve better soothing effects at a specific ratio.

[0132] Comparing Application Example 1 with Application Examples 7-9, it can be seen that when pullulan, tamarind seed polysaccharide and squalane are selected as moisturizers, the three can achieve a synergistic effect in soothing.

[0133] By comparing Application Example 1 with Application Example 10, it can be seen that the moisturizing and emollient composition containing recombinant collagen and the specific moisturizer can achieve a synergistic effect in terms of soothing.

[0134] By comparing Application Example 1 with Comparative Application Examples 1-5, it can be seen that the recombinant collagen, emblica extract, kapok extract, finger orange extract and serine in the moisturizing and moisturizing composition containing recombinant collagen of the present invention cooperate with each other and have a synergistic effect in soothing.

[0135] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0136] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0137] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A moisturizing and skin-moisturizing composition containing recombinant collagen, characterized in that: The moisturizing and moisturizing composition containing recombinant collagen comprises recombinant collagen, emblica fruit extract, kapok flower extract, finger orange extract and serine.

2. The moisturizing and moisturizing composition containing recombinant collagen according to claim 1, characterized in that: The nucleotide sequence encoding the recombinant collagen is the sequence shown in SEQ ID NO:

1.

3. The moisturizing and moisturizing composition containing recombinant collagen according to claim 1 or 2, characterized in that: The moisturizing and moisturizing composition containing recombinant collagen comprises, by weight, 4-8 parts of recombinant collagen, 4-8 parts of emblica extract, 4-12 parts of kapok extract, 2-6 parts of finger lime extract and 10-18 parts of serine.

4. Use of the moisturizing and emollient composition containing recombinant collagen according to any one of claims 1 to 3 in the preparation of cosmetics.

5. The use according to claim 4, characterized in that: The cosmetics include lotions, emulsions, creams, facial masks, essences or gels.

6. The use according to claim 4 or 5, characterized in that: The mass percentage of the moisturizing and moisturizing composition containing recombinant collagen in the cosmetics is 15-25%.

7. A lotion containing recombinant collagen, characterized in that: The lotion comprises, by weight percentage, 120-160 parts of water, 3-11 parts of a moisturizing agent and 24-52 parts of the moisturizing and emollient composition containing recombinant collagen according to any one of claims 1 to 3.

8. The lotion containing recombinant collagen according to claim 7, characterized in that: The moisturizing agent includes any one or a combination of at least two of glycerin, propylene glycol, panthenol, fructose, butylene glycol, 1,2-hexanediol, pullulan, tamarind seed polysaccharide and squalane; Preferably, the moisturizing agent comprises pullulan, tamarind seed polysaccharide and squalane.

9. The lotion containing recombinant collagen according to claim 7 or 8, characterized in that: The moisturizing lotion containing recombinant collagen also includes 1-3 parts of preservatives; Preferably, the preservative includes any one or a combination of at least two of phenoxyethanol, benzyl alcohol, propylparaben, butylparaben, potassium sorbate, chlorphenesin, sodium benzoate, caprylhydroxamic acid or levulinic acid.

10. The method for preparing a lotion containing recombinant collagen according to any one of claims 7 to 9, characterized in that: The preparation method comprises: The moisturizing and emollient composition containing recombinant collagen according to any one of claims 1 to 3, a moisturizer and water are mixed, stirred evenly, and filtered to obtain the lotion containing recombinant collagen.

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