Preparation process and application of deodorant bovine bone paste liquid

Abstract

The preparation process comprises the following steps: propagating a composite fermentation bacterial agent into a composite fermentation bacterial liquid, adding the composite fermentation bacterial liquid into bovine bone paste, carrying out anaerobic fermentation, and after fermentation is completed, carrying out standing precipitation to prepare a carrier auxiliary material, namely the deodorized bovine bone paste liquid; and mixing the deodorized bovine bone paste liquid with bacillus powder and zymophyte powder to prepare the biological organic liquid fertilizer. The biological organic liquid fertilizer is generated by breeding bacillus and zymophyte in the deodorized bovine bone paste liquid, the soil microbial environment and the organic matter content are improved, pathogenic bacteria and plant root-knot nematode are effectively inhibited, and the biological organic liquid fertilizer has the advantages of being environmentally friendly, low in cost and good in application prospect.

Description

Technical Field

[0001] The invention belongs to the technical field of fermentation, and in particular relates to a preparation process and application of deodorized ox bone paste liquid. Background Art

[0002] In agricultural production, increasing soil organic matter and reducing the use of chemical fertilizers are key measures to achieve sustainable agricultural development. Currently, one of the main challenges facing agriculture is how to effectively utilize processing waste resources rich in organic matter, process them into organic fertilizers and apply them to the soil to improve soil fertility, reduce dependence on chemical fertilizers, and achieve sustainable agricultural production.

[0003] In this regard, the recycling of resources by-products from the processing industry is an important approach. For example, cow bone paste is usually rich in organic matter and has a high utilization value. However, when traditional cow bone paste is fermented under natural conditions, it will produce a foul odor due to the action of miscellaneous bacteria and needs to be disposed of by landfill. This method not only has a long-lasting odor, but the leakage of the bottom layer of the fermented liquid will pollute the environment, bringing great ecological risks.

[0004] Although a variety of effective measures have been taken to reduce the environmental hazards of cattle bone paste and have achieved certain results, these measures have not fully solved the problem of effective utilization of cattle bone paste.

[0005] In addition, traditional fertilizers and treatment methods have failed to effectively improve the soil microbial environment, affecting the formation of beneficial bacterial communities in the rhizosphere soil of plants. In addition, the range of inhibition of soil pathogens is limited, and it is impossible to fully respond to the threat of pathogens, making plants vulnerable to diseases. Moreover, traditional methods have little effect on improving soil organic matter levels, which limits the improvement of soil nutrient richness and long-term fertility. Finally, chemical control methods often cause environmental pollution, are costly, and difficult to promote.

[0006] Therefore, the present invention proposes a preparation process and application of deodorized ox bone paste liquid. Summary of the invention

[0007] In order to solve the above technical problems, the present invention proposes a preparation process and application of deodorized cattle bone paste liquid, by which cattle bone paste is fermented specifically by biological bacteria to make organic liquid fertilizer, which is applied to agricultural production, reduces the use of chemical fertilizers, promotes biological organic fertilizers, and promotes environmental protection and sustainable agricultural development.

[0008] In order to achieve the above technical effects, the present invention is implemented through the following technical solutions:

[0009] A preparation process of deodorized ox bone paste liquid, characterized in that it comprises the following steps:

[0010] S1, placing the composite fermentation bacterial agent at a temperature of 20°C to 30°C to expand it into a composite fermentation bacterial liquid;

[0011] S2. Under normal temperature conditions, add 1 to 5 kg of lactic acid compound bacteria to 95 to 99 kg of ox bone paste, mix evenly, and inject into a fermentation tank for anaerobic fermentation. The fermentation time lasts for 3 to 5 days, wherein the water content of the deodorized ox bone paste liquid is 70% to 80%; after the fermentation is completed, the mixture is allowed to stand and settle for 3 to 5 days to prepare the carrier auxiliary material deodorized ox bone paste liquid.

[0012] Furthermore, the composite fermentation bacterial agent in S1 is one or more of yeast, brewer's yeast, acetic acid bacteria, lactic acid bacteria, and Pediococcus acidilactici.

[0013] Furthermore, the anaerobic fermentation temperature in S2 is controlled at 20-30°C.

[0014] Another object of the present invention is to provide an application of deodorized ox bone paste liquid, characterized in that it comprises the following steps:

[0015] (1) preparing a bacillus powder, wherein the water content is 20% to 40%, and the bacillus content is 5 to 10 billion / g; preparing a fermentation bacteria powder, wherein the water content is 20% to 40%, and the fermentation bacteria content is 5 to 10 billion / g;

[0016] (2) Under normal temperature conditions, 1 to 5 kg of Bacillus powder is added to 800 to 1200 kg of carrier auxiliary materials, and then 1 to 5 kg of fermentation bacteria powder is added, mixed and filtered to prepare a biological organic liquid fertilizer.

[0017] The beneficial effects of the present invention are:

[0018] 1. The present invention uses bacillus and fermentation bacteria. These biological functional bacteria can use the organic matter in the deodorized ox bone paste liquid as nutrients to reproduce and form biological organic liquid fertilizer. After being applied to the soil, these bacteria reproduce in the rhizosphere soil of plants to form dominant bacteria, which helps to improve the soil microbial environment;

[0019] 2. The present invention adopts a combination of deodorized ox bone paste liquid, bacillus and fermentation bacteria, which expands the inhibition range of pathogens, increases the organic matter content in the soil, and provides richer nutrition for the soil;

[0020] 3. The deodorized ox bone paste liquid increases the level of soil organic matter, which is not only a source of nutrients for Bacillus and fermentation bacteria, but also can promote the reproduction of these bacteria and establish dominant bacteria in the soil. In addition, fermentation bacteria have strong pathogenic activity against plant root-knot nematodes and effectively inhibit their harm. This biological method is not only environmentally friendly, because it significantly reduces environmental pollution compared to chemical control methods, but also has low cost, easy to promote, and has good application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for describing the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0022] Figure 1 is a flow chart of the preparation process of the present invention; DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] Example 1

[0025] See also Figure 1 As shown, a preparation process of deodorized ox bone paste liquid is characterized by comprising the following steps:

[0026] S1, placing the composite fermentation bacterial agent at a temperature of 20°C to 30°C to expand it into a composite fermentation bacterial liquid;

[0027] S2. Under normal temperature conditions, 3 kg of lactic acid compound bacteria are added to 95 kg of ox bone paste, mixed evenly, and injected into a fermentation tank for anaerobic fermentation. The fermentation time lasts for 5 days, wherein the water content of the deodorized ox bone paste liquid is 70% to 80%; after the fermentation is completed, the mixture is allowed to stand and settle for 5 days to prepare the carrier auxiliary material deodorized ox bone paste liquid.

[0028] The composite fermentation bacterial agent in S1 is one or more of yeast, brewer's yeast, acetic acid bacteria, lactic acid bacteria, and Pediococcus acidilactici.

[0029] The anaerobic fermentation temperature in S2 is controlled at 22°C.

[0030] An application of deodorizing ox bone paste liquid is characterized by comprising the following steps:

[0031] (1) preparing a bacillus powder, wherein the water content is 30% and the bacillus content is 5 billion / g; preparing a fermentation bacteria powder, wherein the water content is 30% and the fermentation bacteria content is 50 / g;

[0032] (2) Under normal temperature conditions, 2 kg of Bacillus powder is added to 1000 kg of carrier auxiliary materials, and then 2 kg of fermentation bacteria powder is added, mixed and filtered to prepare a biological organic liquid fertilizer.

[0033] Example 2

[0034] Field trials in tobacco production are used to evaluate the effects of bio-organic liquid fertilizer on promoting tobacco growth and improving disease resistance.

[0035] This experiment aims to study the effects of bio-organic liquid fertilizer on tobacco leaf growth and disease resistance, especially the control effect on viral disease and leaf spot. The experiment has three treatment groups (group A, group B, group C) and a control group. Groups A, B, and C use organic liquid fertilizers of different concentrations or formulas, and the control group does not use organic liquid fertilizers. Three replicates are set for each group and randomly distributed in the experimental field. In terms of fertilization treatment, organic liquid fertilizers are applied regularly according to the prescribed concentration or formula, and pathogens are artificially inoculated according to experimental needs. Growth observations include regular measurements of growth parameters such as plant height and leaf area. The disease control effect was evaluated by calculating the disease incidence and disease index. The results showed that the control effects of groups A, B, and C on viral diseases were 15.80%, 15.30%, and 7.50%, respectively, and the control effects on leaf spot were 9.65%, 11.65%, and 1.4%, respectively. Group A performed best in growth promotion. Through discussion and analysis, it was found that the reason why Group A showed excellent results was due to its optimized fertilizer formula. Bio-organic liquid fertilizer showed good application prospects and economic benefits in agriculture. The conclusion pointed out that bio-organic liquid fertilizer had significant effects in improving the disease resistance and promoting the growth of tobacco plants, especially the treatment scheme of Group A was economical and effective. Therefore, it is recommended to promote the use of bio-organic liquid fertilizer with Group A formula in actual agricultural production.

[0036] Example 3

[0037] This experiment aims to study the effects of bio-organic liquid fertilizer combined with inorganic fertilizer on the growth, leaf maturity and economic benefits of pepper plants, and to evaluate its application effect in red soil mountain agriculture. The experimental materials include pepper plants, bio-organic liquid fertilizer, inorganic fertilizer and farmyard manure. The experiment has four treatment groups, three of which are treatment groups (treatments A, B, and C) in which bio-organic liquid fertilizer is combined with inorganic fertilizers of different formulas, and another control group. Three replicates are set up in each group and randomly distributed in the experimental field. The fertilization treatment was carried out in an experimental field of 666.6 square meters. The treatment group applied 500 kg of farmyard manure, a total of 4.2 kg of pure N, and different concentrations of bio-organic liquid fertilizer and inorganic fertilizer were applied respectively. Fertilization was carried out twice, in the budding stage and the closing stage of pepper. During the experiment, the growth of pepper plants was regularly observed and recorded, including the stem-leaf angle, leaf SPAD value (relative chlorophyll content), yield, output value, average price and proportion of superior agriculture. Data collection and analysis included recording the changes in the stem-leaf angle of pepper leaves in each group, SPAD values, and calculating the yield, output value, average price, and proportion of superior agriculture. The economic benefit analysis evaluated the input-output ratio of each group, especially the effect of the use of organic liquid fertilizer formula fertilizer, by comparing the yield and output value. The experimental results showed that in the treatment group using biological organic liquid fertilizer, the stem-leaf angle of pepper leaves increased by 1.5-2.94%, the SPAD value of leaves increased by 1.2%-4.1%, the yield, output value, average price, and proportion of superior agriculture increased by 9.7%-11.2%, 16.2%-18.7%, 6.7%-7.6%, and 1.62%-12.04%, respectively. The input-output ratio reached 1:5.6, showing obvious economic benefits. In summary, the combined application of biological organic liquid fertilizer and inorganic fertilizer showed significant positive effects on pepper growth, which not only improved leaf maturity and plant health, but also significantly increased yield and economic benefits. In particular, the use of this formula fertilizer has a high input-output ratio and has broad prospects for promotion and application.

Claims

1. A preparation process for deodorized ox bone paste liquid, characterized in that: The steps include: S1, placing the composite fermentation bacterial agent at a temperature of 20°C to 30°C to expand it into a composite fermentation bacterial liquid; S2. Under normal temperature conditions, add 1 to 5 kg of lactic acid compound bacteria to 95 to 99 kg of ox bone paste, mix evenly, and inject into a fermentation tank for anaerobic fermentation. The fermentation time lasts for 3 to 5 days, wherein the water content of the deodorized ox bone paste liquid is 70% to 80%; after the fermentation is completed, the mixture is allowed to stand and settle for 3 to 5 days to prepare the carrier auxiliary material deodorized ox bone paste liquid.

2. The preparation process of a deodorized ox bone paste liquid according to claim 1, characterized in that: The composite fermentation bacterial agent in S1 is one or more of yeast, brewer's yeast, acetic acid bacteria, lactic acid bacteria, and Pediococcus acidilactici.

3. The preparation process of a deodorized ox bone paste liquid according to claim 1, characterized in that: The anaerobic fermentation temperature in S2 is controlled at 20-30°C.

4. An application of deodorizing ox bone paste liquid, characterized in that: The steps include: (1) preparing a bacillus powder, wherein the water content is 20% to 40%, and the bacillus content is 5 to 10 billion / g; preparing a fermentation bacteria powder, wherein the water content is 20% to 40%, and the fermentation bacteria content is 5 to 10 billion / g; (2) Under normal temperature conditions, 1 to 5 kg of Bacillus powder is added to 800 to 1200 kg of carrier auxiliary materials, and then 1 to 5 kg of fermentation bacteria powder is added, mixed and filtered to prepare a biological organic liquid fertilizer.

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