Compound for promoting hair growth and blacking hair as well as preparation method and application of compound

Abstract

The invention relates to the technical field of shampoo, and discloses a compound for promoting hair growth and blackening as well as a preparation method and application of the compound. 1, 1, 2-pentanediol; yeast / zinc fermentation products; butanediol; ethanol; an apple fruit cell culture extract; a fructus forsythiae extract; a zanthoxylum fruit extract; the solvent is 1, 1, 2-hexanediol; a copper tripeptide-1; a rehmannia root extract; 4-hydroxyacetophenone; an nasturtium officinale leaf / stem extract; according to the invention, the yeast / zinc fermentation product, the pepper fruit extract and the rehmannia root extract are combined, so that the prepared compound does not cause extra stimulation to the scalp when the scalp is damaged and can also have a protective effect on the scalp, and the three raw materials jointly generate an effect; the hair-blacking composition has the advantages that the hair-blacking composition has the effects of diminishing inflammation, promoting blood circulation and deeply nourishing scalp, a healthy environment beneficial to hair root growth is created, and the hair-blacking composition not only has the hair-blacking effect, but also can protect the scalp.

Description

Technical Field

[0001] The invention relates to the technical field of shampoo, in particular to a compound for promoting hair growth and darkening hair, and a preparation method and application thereof. Background Art

[0002] Hair growth and pigmentation is a complex biological process that is influenced by many factors, including genetics, diet, environmental factors, and hormone levels. As we age, many people experience thinning hair, hair loss, or gray hair, which is not only a cosmetic concern, but may also affect personal confidence and mental health.

[0003] Traditionally, people use a variety of natural and chemical products to promote hair growth and delay the appearance of gray hair. However, many traditional hair growth or hair darkening products only address one aspect of the problem, such as only promoting hair growth or only targeting gray hair, and the products cannot protect the scalp. Summary of the invention

[0004] 1. Technical issues to be resolved In view of the deficiencies in the prior art, the present invention provides a compound for promoting hair growth and darkening hair, and a preparation method and application thereof, which has the advantages of darkening hair while protecting the scalp, solving the problem that traditional hair darkening products only darken hair but cannot protect the scalp.

[0005] (II) Technical solution To achieve the above-mentioned purpose, the present invention provides the following technical scheme: a compound for promoting hair growth and darkening hair, comprising the following raw materials: water; 1,2-pentanediol; yeast / zinc fermentation product; butylene glycol; ethanol; apple fruit cell culture extract; forsythia extract; Zanthoxylum fruit extract; 1,2-hexanediol; tripeptide-1 copper; Rehmannia root extract; p-hydroxyacetophenone; watercress leaf / stem extract; ethylhexylglycerin; The raw material content ratio is: water 93.415%; 1,2-pentanediol 2%; yeast / zinc fermentation product 1.972%; butylene glycol 1.23%; ethanol 0.45%; apple fruit cell culture extract 0.3%; forsythia extract 0.216%; Zanthoxylum fruit extract 0.144%; 1,2-hexanediol 0.02%; tripeptide-1 copper 0.02%; rehmannia root extract 0.002%; p-hydroxyacetophenone 0.006%; watercress leaf / stem extract 0.005%; ethylhexylglycerin 0.002%.

[0006] Preferably, a method for preparing a compound for promoting hair growth and darkening hair comprises the following steps: Step 1: Raw material pretreatment: preparation of yeast / zinc fermentation products and extraction of some raw materials; Step 2: Weigh the raw materials: according to the raw material ratio, use a micro electronic scale to weigh each raw material; Step 3: Clean equipment: Clean and disinfect all tools used in the preparation process; Step 4, preparing the aqueous phase: adding water into a beaker and heating it, then adding 1,2-pentanediol and butanediol into the beaker and stirring evenly until completely dissolved, then adding apple fruit cell culture extract, forsythia extract and watercress leaf / stem extract; Step 5, oil phase preparation: mix Zanthoxylum bungeanum fruit extract, tripeptide-1 copper and ethylhexylglycerin in another beaker, and heat and stir at the same temperature; Step 6: Mix the water phase and the oil phase: add the oil phase to the water phase, then add 1,2-hexanediol, and homogenize using a high-speed homogenizer; Step 7. Add low temperature and active substances: lower the temperature of the heater, add yeast / zinc fermentation product, Rehmannia root extract, stir evenly, and then add p-hydroxyacetophenone and ethanol; Step 8. Adjust pH: Use a pH regulator to adjust the pH value to the optimal range accepted by the skin to make a complex.

[0007] Preferably, the raw materials to be extracted in step 1 include: apple fruit cell culture extract, forsythia extract, Zanthoxylum fruit extract, Rehmannia root extract and watercress leaf / stem extract.

[0008] Preferably, the production process of the yeast / zinc fermentation product is: S1.1. Selection and cultivation of yeast: baker's yeast was selected to cultivate yeast in YPD medium, and the pH of YPD medium was adjusted to 5.0, the incubator temperature was set between 29℃-30℃, and 99% oxygen at a flow rate of 10ml / min was directly introduced into the medium through an oxygen bottle, and the cultivation time was set to 18-24 hours; S1.2. Yeast collection: After the yeast culture time reaches 18 hours, use a spectrophotometer to regularly detect the OD value of the culture solution. When the OD value reaches 0.95-1.0, collect the cells by centrifuge; S1.3. Selection and addition of zinc salt: Add 0.2 g of zinc oxide to the yeast culture and stir evenly at 30° C. to complete the production of the yeast / zinc fermentation product.

[0009] Preferably, the preparation process of the Zanthoxylum bungeanum fruit extract is: S2.1. Raw material preparation: Select fresh, disease-free and insect-free dark green pepper fruits and remove impurities from the pepper fruits; S2.2, crushing: put the pepper fruits into a crusher and crush them into 1mm particles; S2.3, extraction: the crushed Zanthoxylum bungeanum fruit was mixed with ethanol and water in a ratio of 1:2:3, the mixture was stirred evenly and soaked for 6 hours, and after the soaking was completed, the mixture was treated with an ultrasonic device for 30 minutes; S2.4, Filtration: After ultrasonic treatment, the mixed solution is centrifuged at 1000 rpm for 45 min. After centrifugation, it is allowed to stand for 2 hours. The upper clear layer is filtered using a triangular funnel and filter paper. The filtered liquid is the Zanthoxylum fruit extract.

[0010] Preferably, the preparation process of the Rehmannia root extract is: S3.1. Raw material preparation: Select pest-free and mature Rehmannia root as raw material and wash the root; S3.2, Slice: Cut the washed Rehmannia root into 1-1.5 mm slices; S3.3, extraction: mix the Rehmannia root slices with ethanol in a ratio of 1:4, and then transfer to a hot reflux extractor, set the temperature of the hot reflux extractor to 75°C-80°C, and set the extraction time to 10h-12h; S3.4, Filtration: After the extraction is completed, turn off the heat source and collect the extract after it cools down; S3.5. Concentration: The extract is concentrated to 1 / 5 of the original volume of the extract by heating at 98° C. through reduced pressure distillation to obtain the Rehmannia root extract.

[0011] Preferably, all tools used in the preparation process in step 3 are cleaned and sterilized, and the tools include a beaker, a magnetic stirrer, a homogenizing container, a pipette, a triangular funnel and a sealed bottle; in step 4, the water phase is prepared by adding water to the beaker and heating the temperature to 60-70° C., and the heating temperature for the oil phase preparation in step 5 is also 60-70° C., and in step 6, the homogenizing speed of the high-speed homogenizer is set to 100 revolutions per minute, and the setting time is 6 minutes.

[0012] Preferably, in step seven, adding low temperature and active substances: the heating temperature is reduced to 40-45°C.

[0013] Preferably, in step eight, a pH meter is used to detect the pH value of the complex, and citric acid or 2% sodium hydroxide is used to adjust the pH value to a range of 5-6.

[0014] The invention discloses an application of a compound for promoting hair growth and darkening hair. The compound prepared by the preparation method is used for preventing hair loss, reducing scalp oil and protecting the scalp.

[0015] Compared with the prior art, the present invention provides a compound for promoting hair growth and dark hair, and a preparation method and application thereof, which have the following beneficial effects: 1. The present invention combines yeast / zinc fermentation products, Zanthoxylum bungeanum fruit extract and Rehmannia root extract to make the prepared complex not cause additional stimulation to the scalp when the scalp is damaged, but also have a protective effect on the scalp. The effects produced by these three raw materials together can play the role of anti-inflammation, blood circulation promotion and deep nourishment for the scalp, create a healthy environment conducive to the growth of hair roots, so as to achieve scalp care from the inside out. The present invention nourishes the scalp and hair roots from the inside of the blood vessels through this method of nourishing yin and tonifying the kidney, and promotes the growth of black hair from the scalp hair roots. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 Create a flow chart for the composite of the present invention; Figure 2 This is a diagram of yeast culture of the present invention. DETAILED DESCRIPTION

[0017] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0018] See also Figure 1 - Figure 2 , a hair growth promoting complex, including the following ingredients: water; 1,2-pentanediol; yeast / zinc ferment; butylene glycol; ethanol; apple fruit cell culture extract; forsythia suspensa extract; zanthoxylum bungeanum fruit extract; 1,2-hexanediol; tripeptide-1 copper; rehmannia glutinosa root (Chinese rehmannia root) extract; p-hydroxyacetophenone; watercress leaf / stem extract; ethylhexylglycerin; The raw material content ratio and its corresponding function are: Water 93.415%; Function: As a solvent, water is the basis of most shampoo formulas, used to dilute and deliver other ingredients; 1,2-Pentanediol 2%; Function: As a solvent and humectant, it helps to adjust the viscosity and moisturizing properties of the complex; Saccharomyces / zinc fermentation product 1.972%; Function: Has anti-inflammatory and antioxidant properties, helps strengthen hair roots and promotes scalp health; Butanediol 1.23%; Function: Acts as a wetting agent and solvent to help other raw materials mix and distribute better; Ethanol 0.45%; Function: Acts as a solvent and preservative to help stabilize the compound and extend its shelf life; Apple fruit cell culture extract 0.3%; Function: Rich in antioxidants and vitamins, helps protect and repair damaged hair; Forsythia suspensa extract 0.216%; Function: Has antibacterial and anti-inflammatory properties to help soothe the scalp; Zanthoxylum bungeanum fruit extract 0.144%; Function: Promote blood circulation and help improve scalp microcirculation; 1,2-Hexanediol 0.02%; Function: Acts as a humectant to help maintain moisture in the scalp and hair; Tripeptide-1 copper 0.02%; Function: Helps stimulate collagen production and strengthen hair structure; Rehmannia root extract 0.002%; Function: Nourishes the kidneys, promotes blood circulation, and helps improve hair quality and growth; p-Hydroxyacetophenone 0.006%; Function: Acts as an antioxidant, protecting complex and hair from free radical damage; Watercress leaf / stem extract 0.005%; Function: Rich in vitamins and minerals, helps nourish scalp and hair; Ethylhexylglycerin 0.002%.

[0019] Function: Acts as a humectant and skin conditioning agent to help maintain the moisture balance of the scalp.

[0020] A method for preparing a compound for promoting hair growth and darkening hair comprises the following steps: Step 1: Raw material pretreatment: preparation of yeast / zinc fermentation products and extraction of some raw materials; Step 2: Weigh the raw materials: according to the raw material ratio, use a micro electronic scale to weigh each raw material; Step 3: Clean equipment: Clean and disinfect all tools used in the preparation process; Step 4, preparing the aqueous phase: adding water into a beaker and heating it, then adding 1,2-pentanediol and butanediol into the beaker and stirring evenly until completely dissolved, then adding apple fruit cell culture extract, forsythia extract and watercress leaf / stem extract; Step 5, oil phase preparation: mix Zanthoxylum bungeanum fruit extract, tripeptide-1 copper and ethylhexylglycerin in another beaker, and heat and stir at the same temperature; Step 6: Mix the water phase and the oil phase: add the oil phase to the water phase, then add 1,2-hexanediol, and homogenize using a high-speed homogenizer; Step 7. Add low temperature and active substances: lower the temperature of the heater, add yeast / zinc fermentation product, Rehmannia root extract, stir evenly, and then add p-hydroxyacetophenone and ethanol; Step 8. Adjust pH: Use a pH regulator to adjust the pH value to the optimal range accepted by the skin to make a complex.

[0021] The raw materials to be extracted in step 1 include: apple fruit cell culture extract, forsythia extract, Zanthoxylum bungeanum fruit extract, rehmannia root extract and watercress leaf / stem extract.

[0022] The production process of yeast / zinc fermentation products: S1.1. Selection and cultivation of yeast: baker's yeast was selected to cultivate yeast in YPD medium, and the pH of YPD medium was adjusted to 5.0, the incubator temperature was set between 29℃-30℃, and 99% oxygen at a flow rate of 10ml / min was directly introduced into the medium through an oxygen bottle, and the cultivation time was set to 18-24 hours; S1.2. Yeast collection: After the yeast culture time reaches 18 hours, use a spectrophotometer to regularly detect the OD value of the culture solution. When the OD value reaches 0.95-1.0, collect the cells by centrifuge. For yeast culture diagram, please refer to Figure 2 ; S1.3. Selection and addition of zinc salt: Add 0.2 g of zinc oxide to the yeast culture and stir evenly at 30° C. to complete the production of the yeast / zinc fermentation product.

[0023] The production process of Zanthoxylum bungeanum fruit extract is as follows: S2.1. Raw material preparation: Select fresh, disease-free and insect-free dark green pepper fruits and remove impurities from the pepper fruits; S2.2, crushing: put the pepper fruits into a crusher and crush them into 1mm particles; S2.3, extraction: the crushed Zanthoxylum bungeanum fruit was mixed with ethanol and water in a ratio of 1:2:3, the mixture was stirred evenly and soaked for 6 hours, and after the soaking was completed, the mixture was treated with an ultrasonic device for 30 minutes; S2.4, Filtration: After ultrasonic treatment, the mixed solution is centrifuged at 1000 rpm for 45 min. After centrifugation, it is allowed to stand for 2 hours. The upper clear layer is filtered using a triangular funnel and filter paper. The filtered liquid is the Zanthoxylum fruit extract.

[0024] The production process of Rehmannia root extract is: S3.1. Raw material preparation: Select pest-free and mature Rehmannia root as raw material and wash the root; S3.2, Slice: Cut the washed Rehmannia root into 1-1.5 mm slices; S3.3, extraction: mix the Rehmannia root slices with ethanol in a ratio of 1:4, and then transfer to a hot reflux extractor, set the temperature of the hot reflux extractor to 75°C-80°C, and set the extraction time to 10h-12h; S3.4, Filtration: After the extraction is completed, turn off the heat source and collect the extract after it cools down; S3.5. Concentration: The extract is concentrated to 1 / 5 of the original volume of the extract by heating at 98° C. through reduced pressure distillation to obtain the Rehmannia root extract.

[0025] All tools used in the preparation process in step 3 are cleaned and sterilized, including a beaker, a magnetic stirrer, a homogenizing container, a pipette, a triangular funnel and a sealed bottle; in step 4, the water phase is prepared by adding water to the beaker and heating the temperature to 60-70° C. The heating temperature for the oil phase preparation in step 5 is also 60-70° C. In step 6, the homogenizing speed of the high-speed homogenizer is set to 100 rpm, and the setting time is 6 minutes.

[0026] Step 7. Add low temperature and active substances: Reduce the heating temperature to 40-45℃.

[0027] Step 8: Use a pH meter to detect the pH value of the complex, and use citric acid or 2% sodium hydroxide to adjust the pH value to a range of 5-6.

[0028] The invention discloses an application of a compound for promoting hair growth and darkening hair. The compound prepared by the preparation method is used for preventing hair loss, reducing scalp oil and protecting the scalp.

[0029] Example 1 A laboratory prepared shampoo according to the following raw material formula: water 93.415%; 1,2-pentanediol 2%; yeast / zinc fermentation product 1.972%; butylene glycol 1.23%; ethanol 0.45%; apple fruit cell culture extract 0.3%; forsythia suspensa extract 0.216%; Zanthoxylum bungeanum fruit extract 0.144%; 1,2-hexanediol 0.02%; tripeptide-1 copper 0.02%; rehmannia root extract 0.002%; p-hydroxyacetophenone 0.006%; watercress leaf / stem extract 0.005%; ethylhexylglycerin 0.002%, and the shampoo was prepared according to the production steps of the present invention.

[0030] Example 2 A laboratory prepared shampoo according to the following raw material formula: water 93.415%; 1,2-pentanediol 2%; yeast / zinc fermentation product 1.2%; butylene glycol 1.23%; ethanol 0.45%; apple fruit cell culture extract 0.3%; forsythia suspensa extract 0.96%; Zanthoxylum bungeanum fruit extract 0.29%; 1,2-hexanediol 0.02%; tripeptide-1 copper 0.02%; Rehmannia glutinosa root extract 0.01%; p-hydroxyacetophenone 0.006%; watercress leaf / stem extract 0.005%; ethylhexylglycerin 0.002%, and prepared shampoo according to the preparation steps of the present invention.

[0031] Example 3 A laboratory prepared shampoo according to the following raw material formula: water 93.415%; 1,2-pentanediol 2%; yeast / zinc fermentation product 2.072%; butylene glycol 1.23%; ethanol 0.45%; apple fruit cell culture extract 0.3%; forsythia suspensa extract 0.188%; Zanthoxylum bungeanum fruit extract 0.111%; 1,2-hexanediol 0.02%; tripeptide-1 copper 0.02%; rehmannia root extract 0.0016%; p-hydroxyacetophenone 0.006%; watercress leaf / stem extract 0.005%; ethylhexylglycerin 0.002%, and the preparation steps of the present invention were followed.

[0032] Comparative Example 1 A laboratory prepared shampoo according to the following raw material formula: water 93.415%; 1,2-pentanediol 2%; butylene glycol 2.23%; ethanol 0.45%; apple fruit cell culture extract 2.3%; forsythia suspensa extract 0.216%; Zanthoxylum bungeanum fruit extract 0.144%; 1,2-hexanediol 0.02%; tripeptide-1 copper 0.02%; Rehmannia glutinosa root extract 0.002%; p-hydroxyacetophenone 0.006%; watercress leaf / stem extract 0.005%; ethylhexylglycerin 0.002%, and the shampoo was prepared according to the production steps of the present invention.

[0033] Comparative Example 2 A laboratory prepared shampoo according to the following raw material formula: water 93.415%; 1,2-pentanediol 2%; yeast / zinc fermentation product 1.972%; butylene glycol 1.23%; ethanol 0.45%; apple fruit cell culture extract 0.3%; forsythia suspensa extract 0.216%; 1,2-hexanediol 0.02%; tripeptide-1 copper 0.02%; rehmannia root extract 0.002%; p-hydroxyacetophenone 0.006%; watercress leaf / stem extract 0.105%; ethylhexylglycerin 0.002%, and followed the production steps of the present invention.

[0034] Comparative Example 3 A laboratory prepared shampoo according to the following raw material formula: water 93.415%; 1,2-pentanediol 2%; yeast / zinc fermentation product 1.972%; butylene glycol 1.23%; ethanol 0.45%; apple fruit cell culture extract 0.4%; forsythia suspensa extract 0.216%; Zanthoxylum bungeanum fruit extract 0.144%; 1,2-hexanediol 0.02%; tripeptide-1 copper 0.02%; p-hydroxyacetophenone 0.006%; watercress leaf / stem extract 0.005%; ethylhexylglycerin 0.002%, and followed the production steps of the present invention.

[0035] The effects of the finished products made according to the raw material formulations of Examples 1-3 and Comparative Examples 1-3 according to the steps of the present invention are listed in the following table:

[0036] It can be seen from the above table that the finished products produced by different formulas of Examples 1-3 and Comparative Examples 1, 2, and 3 have different effects. In Examples 1-3, yeast / zinc fermentation products, Zanthoxylum bungeanum fruit extract and Rehmannia root extract are used, while in Comparative Examples 1, 2, and 3, yeast / zinc fermentation products, Zanthoxylum bungeanum fruit extract and Rehmannia root extract are removed in order.

[0037] According to the above table data, the beneficial effects of Examples 1-3 in cleaning ability, moisturizing effect, repair ability and scalp health are all above 97%, and the user feedback is also rated as "excellent". Compared with Comparative Examples 1, 2, and 3, Comparative Example 1 lacks yeast / zinc fermentation products and has a serious decline in repair ability, and Comparative Example 2 lacks Zanthoxylum bungeanum fruit extract and has different degrees of decline in moisturizing effect, repair ability and scalp health. The performance of Comparative Example 3 has declined in various indicators, and the user feedback of Comparative Examples 1, 2, and 3 is also rated as "medium".

[0038] The results showed that the absence of yeast / zinc ferment, Zanthoxylum fruit extract and Rehmannia root extract in the shampoo formula affected the overall performance of the complex. The combination of these three substances has a synergistic effect, which jointly enhances the cleansing ability, moisturizing effect and repair ability of the shampoo, thereby promoting scalp health.

[0039] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A compound for promoting hair growth and darkening hair, characterized by: Contains the following ingredients: Water; 1,2-pentanediol; Saccharomyces / zinc ferment; Butylene glycol; Ethanol; Apple fruit cell culture extract; Forsythia suspensa extract; Zanthoxylum bungeanum fruit extract; 1,2-hexanediol; Copper Tripeptide-1; Rehmannia glutinosa root extract; 4-Hydroxyacetophenone; Nasturtium officinale leaf / stem extract; Ethylhexylglycerin; The raw material content ratio is: 93.415% water; 2% 1,2-pentanediol; 1.972% yeast / zinc fermentation product; 1.23% butylene glycol; 0.45% ethanol; 0.3% apple fruit cell culture extract; 0.216% forsythia extract; 0.144% zanthoxylum fruit extract; 0.02% 1,2-hexanediol; 0.02% tripeptide-1 copper; 0.002% rehmannia root extract; 0.006% p-hydroxyacetophenone; 0.005% watercress leaf / stem extract; and 0.002% ethylhexylglycerin.

2. A method for preparing a compound for promoting hair growth and darkening hair, characterized in that: The following steps are involved: Step 1: Raw material pretreatment: preparation of yeast / zinc fermentation products and extraction of some raw materials; Step 2: Weigh the raw materials: according to the raw material ratio formula, use a micro electronic scale to weigh each raw material; Step 3: Clean equipment: Clean and disinfect all tools used in the preparation process; Step 4: Prepare the aqueous phase: add water to a beaker and heat it. Then add 1,2-pentanediol and butylene glycol to the beaker and stir until completely dissolved. Then add the apple fruit cell culture extract, forsythia suspensa extract, and watercress leaf / stem extract. Step 5, oil phase preparation: mix Zanthoxylum bungeanum fruit extract, tripeptide-1 copper and ethylhexylglycerin in another beaker, and heat and stir at the same temperature; Step 6: Mix the water phase and the oil phase: Add the oil phase to the water phase, then add 1,2-hexanediol, and homogenize using a high-speed homogenizer; Step 7: Add low temperature and active substances: Lower the temperature of the heater, add the yeast / zinc fermentation product and Rehmannia root extract, stir well, and then add p-hydroxyacetophenone and ethanol; Step 8. Adjust pH: Use a pH regulator to adjust the pH value to the optimal range accepted by the skin. The complex is made.

3. The method for preparing a hair growth promoting and darkening hair compound according to claim 2, characterized in that: The raw materials to be extracted in the step 1 include: apple fruit cell culture extract, forsythia suspensa extract, zanthoxylum bungeanum fruit extract, rehmannia root extract and watercress leaf / stem extract.

4. The method for preparing a hair growth promoting and darkening hair compound according to claim 2, characterized in that: The production process of the yeast / zinc fermentation product: S1.

1. Yeast selection and cultivation: Baker's yeast was cultured in YPD medium. The pH of the YPD medium was adjusted to 5.

0. The incubator temperature was set between 29°C and 30°C. 99% oxygen was directly introduced into the medium at a flow rate of 10 ml / min via an oxygen cylinder. The culture time was set to 18-24 hours. S1.

2. Yeast collection: After the yeast culture reaches 18 hours, regularly monitor the OD value of the culture medium using a spectrophotometer. When the OD value reaches 0.95-1.0, collect the cells by centrifuge. S1.

3. Selection and addition of zinc salt: Add 0.2 g of zinc oxide to the yeast culture and stir evenly at 30° C. to prepare the yeast / zinc fermentation product.

5. The method for preparing a hair growth promoting and darkening hair compound according to claim 3, characterized in that: The preparation process of the Zanthoxylum bungeanum fruit extract is as follows: S2.

1. Raw material preparation: Select fresh, pest-free, dark green Zanthoxylum bungeanum fruits and remove impurities from the fruits; S2.2, crushing: Place the Zanthoxylum bungeanum fruits in a crusher and crush them into 1 mm particles; S2.3, Extraction: Mix the crushed Zanthoxylum bungeanum berries with ethanol and water in a ratio of 1:2:3, stir well, and soak for 6 hours. After soaking, treat the mixture with an ultrasonic device for 30 minutes; S2.

4. Filtration: After ultrasonic treatment, the mixture was centrifuged at 1000 rpm for 45 min. After centrifugation, it was allowed to stand for 2 hours. The upper clear layer was filtered using a triangular funnel and filter paper. The filtered liquid was the Zanthoxylum bungeanum fruit extract.

6. The method for preparing a hair growth promoting and darkening hair compound according to claim 3, characterized in that: The preparation process of the Rehmannia root extract is as follows: S3.

1. Raw material preparation: Select pest-free, mature Rehmannia root as the raw material and wash it thoroughly; S3.

2. Slice: Cut the washed Rehmannia root into 1-1.5 mm slices; S3.3, Extraction: Mix the Rehmannia root slices with ethanol in a ratio of 1:4, transfer to a hot reflux extractor, set the temperature of the hot reflux extractor to 75°C-80°C, and set the extraction time to 10h-12h; S3.4, Filtration: After the extraction is complete, turn off the heat source and collect the extract after it cools down; S3.

5. Concentration: The extract is concentrated by heating at 98° C. to 1 / 5 of the original volume of the extract by means of reduced pressure distillation to obtain the Rehmannia root extract.

7. The method for preparing a hair growth promoting and darkening hair compound according to claim 2, characterized in that: All tools used in the preparation process in step 3 are cleaned and sterilized, including a beaker, a magnetic stirrer, a homogenizing container, a pipette, a triangular funnel, and a sealed bottle. In step 4, the aqueous phase is prepared by adding water to the beaker and heating it to a temperature of 60-70° C. The heating temperature for preparing the oil phase in step 5 is also 60-70° C. In step 6, the homogenization speed of the high-speed homogenizer is set to 100 revolutions per minute, and the setting time is 6 minutes.

8. The method for preparing a hair growth promoting and darkening hair compound according to claim 2, characterized in that: Step 7: Adding low temperature and active substances: reduce the heating temperature to 40-45°C.

9. The method for preparing a hair growth promoting and darkening compound according to claim 2, characterized in that: In step eight, a pH meter is used to detect the pH value of the complex, and citric acid or 2% sodium hydroxide is used to adjust the pH value to a range of 5-6.

10. An application of a compound for promoting hair growth and darkening hair, characterized in that: The complex prepared by the preparation method according to claim 2 is used for preventing hair loss, reducing scalp oiliness and protecting the scalp.

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