Camellia diester edible oil capable of assisting in reducing blood fat and preparation method of camellia diester edible oil

Abstract

The invention belongs to the field of edible oil preparation, and particularly relates to camellia diester edible oil assisting in reducing blood fat and a preparation method of the camellia diester edible oil. Walnuts, peas and sweet corn kernels are ground into thick liquid and then are subjected to sufficient enzymolysis, the obtained nutritional rapeseed oil is dispersed in mixed edible oil, and the prepared nutritional camellia diester oil mixture is rich in nutrition; the plant extract obtained by ultrasonic extraction is effectively adsorbed by the mixed jelly, and the obtained nano particle and nutrient camellia diester oil mixture assisting in reducing blood fat and promoting metabolism has an effective blood fat reducing effect; the antioxidant stability maintaining composition and the nanoparticles assisting in reducing blood fat and promoting metabolism are uniformly dispersed in the nutritional camellia diester oil mixture, and the prepared camellia diester edible oil assisting in reducing blood fat is rich in nutrition, does not increase the blood fat of a body after being eaten for a long time, does not damage the liver and kidney, and is not easy to deteriorate.

Description

Technical Field

[0001] The invention belongs to the field of edible oil preparation, and specifically relates to camellia diester edible oil for assisting in lowering blood lipids and a preparation method thereof. Background Art

[0002] Camellia oleifera edible oil is a kind of edible oil extracted from the seeds of Camellia oleifera of the Theaceae family. It is a healthy edible oil prepared by converting triglycerides in ordinary edible oil into diglycerides. Its main component is diglyceride. Camellia oleifera edible oil is rich in diglycerides and abundant unsaturated fatty acids such as linoleic acid and linolenic acid. It is resistant to high temperatures and is suitable for high-temperature cooking.

[0003] The content of unsaturated fatty acids in camellia diester edible oil exceeds that of olive oil, ranking first among all edible oils. It can effectively remove free radicals in the body, delay cell aging, keep the skin youthful, promote gastrointestinal motility, help digest food, and enhance the body's ability to absorb nutrients.

[0004] At present, the existing edible oil preparation technology has the following problems: First, long-term consumption of existing edible oil leads to an increase in low-density lipoprotein cholesterol in the blood, which affects the metabolism of the liver and kidneys, resulting in a decrease in the liver and kidneys' ability to metabolize fat, further increasing blood lipids; Second, the existing edible oil pressing process causes the loss of nutritional components such as carotene, chlorophyll, phytosterols, and vitamin E, resulting in a reduction in flavor and aroma; Third, after the existing edible oil is opened for too long, it is easy to oxidize and become rancid in light, high temperature and air, the color becomes darker and deeper, the turbidity and transparency decrease, and sediments and suspended matter appear. Summary of the invention

[0005] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides a camellia diester edible oil for assisting in lowering blood lipids and a preparation method thereof. In order to solve the problems that the existing edible oils lead to increased low-density lipoprotein cholesterol in the blood, damage the metabolism of the liver and kidneys, are prone to oxidation and rancidity, and have reduced turbidity and transparency during long-term storage after long-term consumption, the present invention uses a nutritious camellia diester oil mixture, auxiliary blood lipid-lowering and metabolism-promoting nanoparticles, and an antioxidant stabilizing composition to mix. The prepared camellia diester edible oil for assisting in lowering blood lipids is rich in nutrition, has a unique aroma, can repair damaged liver and kidneys, will not increase blood lipids in the body after long-term consumption, is not prone to deterioration during long-term storage, and has strong stability.

[0006] In order to achieve the above object, the technical solution adopted by the present invention is as follows: the present invention proposes a camellia diester edible oil that assists in lowering blood lipids, and the raw materials for preparing the camellia diester edible oil that assists in lowering blood lipids specifically include the following components in parts by weight: 90-110 parts of nutritious camellia diester oil mixture, 30-44 parts of nanoparticles that assist in lowering blood lipids and promoting metabolism, 7-11 parts of bamboo leaf antioxidants, and 4-8 parts of disodium ethylenediaminetetraacetate.

[0007] Preferably, the raw materials for preparing the nutritious camellia diester oil mixture include the following components in parts by weight: 10-12 parts of walnuts, 7-11 parts of peas, 6-9 parts of sweet corn kernels, 33-38 parts of rapeseed oil, 42-53 parts of camellia diester oil, 37-46 parts of red pine seed oil, 39-48 parts of sunflower seed oil, and 7-12 parts of soluble soybean polysaccharides; the raw materials for preparing the auxiliary lipid-lowering and metabolism-promoting nanoparticles include the following components in parts by weight: 14-18 parts of cassia seeds, 16-19 parts of polygonum multiflorum, 4-9 parts of eucommia, 7-11 parts of dodder seeds, and 26-31 parts of sodium starch phosphate.

[0008] Preferably, the method for preparing the nutritious camellia diester oil mixture specifically comprises the following steps: S1. Put walnuts, peas and sweet corn kernels into a pulping machine with a power of 1.5-1.8 kW, the pulping speed is 3000 r / min, the pulping temperature is 28-33°C, and the pulping time is 10-15 min. After pulping, put them into an enzymolysis tank with a power of 1.8-2.2 kW with cellulase and bromelain, the enzymolysis temperature is 50-55°C, the enzymolysis time is 2-2.5 h, the enzymolysis speed is 70-90 r / min, stir and enzymolyze to obtain a nutrient mixture; S2, the nutrient mixture prepared by S1 and rapeseed oil are placed in an ultrasonic extractor with a power of 1.5 kW, an ultrasonic frequency of 20 kHz, an ultrasonic extraction temperature of 40°C, and an ultrasonic extraction time of 30 min. After ultrasonic extraction, the mixture is placed in a high-speed centrifuge with a power of 1.6 kW, a centrifugal temperature of 2°C, a centrifugal speed of 22000 r / min, and a centrifugal time of 15 min. After centrifugation, the supernatant is taken to obtain nutrient rapeseed oil; S3, putting camellia diester oil, Korean pine seed oil and sunflower seed oil into a stirring tank with a power of 1.5-1.8 kW, stirring at a temperature of 25-28° C., a stirring speed of 130-150 r / min, a stirring time of 15-25 min, stirring and mixing to obtain a mixed edible oil; S4. Put the nutritious rapeseed oil prepared by S2, soluble soybean polysaccharide and the mixed edible oil prepared by S3 into a high-speed shear disperser with a power of 1.5-1.8 kW, the high-speed shear dispersion temperature is 23-28°C, the high-speed shear dispersion time is 12-16min, the high-speed shear dispersion speed is 3000-4000 r / min, and high-speed shear dispersion is performed to obtain a nutritious camellia diester oil mixture.

[0009] Preferably, the preparation method of the auxiliary lipid-lowering metabolic agent specifically comprises the following steps: L1. Put Cassia seed, Polygonum multiflorum, Eucommia ulmoides and Cuscuta seed into a pulverizer with a power of 1.4-1.8 kW, the pulverization temperature is 25-28°C, the pulverization time is 30-40 min, and the pulverization speed is 1200-1500 r / min. After pulverization, put them into an ultrasonic extractor with a power of 1.5 kW with 70% ethanol solution, the ultrasonic frequency is 20 kHz, the ultrasonic extraction temperature is 40°C, and the ultrasonic extraction time is 30 min. After ultrasonic extraction, obtain a plant extract; L2, put the plant extract prepared in L1 and sodium starch phosphate into a stirring tank with a power of 1.2-1.4 kW, stir at a temperature of 50-60°C, a stirring speed of 120-140 r / min, and a stirring time of 18-23 min. After stirring and mixing, place at a temperature of 25-28°C for 1 h to obtain a mixed colloid; L3. Place the mixed colloid prepared in L2 into a vacuum freeze-nano spray dryer with a power of 2.3-2.8 kW, with a vacuum degree of -0.05 MPa, a freeze-nano spray drying temperature of -40°C, a freeze-nano spray drying particle size of 100 nm, and a freeze-nano spray drying time of 2.5-3 h to obtain nanoparticles that assist in lowering blood lipids and promoting metabolism.

[0010] Furthermore, in step L1, the weight ratio of the 70% ethanol solution to Cassia seed is 0.9-1.2:1.

[0011] The present invention also proposes a method for preparing camellia diester edible oil that assists in lowering blood lipids, which specifically comprises the following steps: Step 1, putting bamboo leaf antioxidants and disodium ethylenediaminetetraacetic acid into a stirring tank with a power of 1.2-1.4 kW, stirring at a temperature of 20-26° C., a stirring speed of 150-180 r / min, and a stirring time of 8-12 min, and mixing to obtain an antioxidant stabilizing composition; Step 2: Add the lipid-lowering and metabolism-promoting nanoparticles into the antioxidant and stabilizing composition prepared in step 1, stir at a temperature of 20-26° C., a stirring speed of 150-180 r / min, and a stirring time of 10-14 min to obtain a mixed preparation; Step 3: Disperse the mixed preparation prepared in step 2 in the nutritious camellia diester oil mixture, put it into an ultrasonic agitator with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min to obtain camellia diester edible oil that assists in lowering blood lipids.

[0012] The beneficial effects achieved by the present invention are as follows: The invention adopts walnut, pea and sweet corn kernels to grind, and then uses cellulase and bromelain to fully enzymolyze, and uses rapeseed oil to extract the obtained nutrient mixture, and takes the supernatant after centrifugation. The prepared nutrient rapeseed oil contains rich carotene, chlorophyll, phytosterol and vitamin E, and the mixed edible oil contains camellia diester oil, red pine seed oil and sunflower seed oil. The camellia diester oil contains rich unsaturated fatty acids, the red pine seed oil has the fragrance of red pine seeds, and the anthocyanins contained in it have the effects of lowering blood lipids and protecting cardiovascular and cerebrovascular vessels. The sunflower seed oil has a fragrant smell and contains a large amount of linoleic acid, which can reduce the accumulation of cholesterol in the blood. The nutrient rapeseed oil and soluble soybean polysaccharide are dispersed in the mixed edible oil by high-speed shearing, the soluble soybean polysaccharide increases the intake of dietary fiber, and the nutrient rapeseed oil is stably dispersed in the mixed edible oil, and the prepared nutrient camellia diester oil mixture has high nutritional value and stability; Cassia seed, Polygonum multiflorum, Eucommia ulmoides and Cuscuta australis are crushed and then simmered with 70% ethanol solution Extraction is performed, and the obtained plant extract contains cassia bark, cassia glycoside, rhamnolide, eucommia glycoside, aucubin, coumarin, and dodder glycoside, which can repair the liver, promote liver fat metabolism, inhibit the synthesis of cholesterol in the body, and convert it into bile acid, thereby enhancing the discharge of bile acid from the intestine and reducing the body's absorption rate of fatty acids. The mixed colloid contains a three-dimensional network structure, which effectively adsorbs the plant extract. After freeze-nano spray drying, the auxiliary lipid-lowering and metabolism-promoting nanoparticles are obtained, which have a small particle size and are evenly dispersed, and cooperate with the nutritious camellia diester oil mixture to achieve an effective lipid-lowering effect; the antioxidant stabilizing composition obtained by mixing bamboo leaf antioxidants and disodium ethylenediaminetetraacetic acid is dispersed in the nutritious camellia diester oil mixture with the auxiliary lipid-lowering and metabolism-promoting nanoparticles. The prepared auxiliary lipid-lowering camellia diester edible oil is rich in nutrition, has a unique aroma, promotes the metabolic function of the liver and kidney, can repair damaged liver and kidney, will not increase the body's blood lipids after long-term consumption, and is not prone to deterioration during long-term storage and has strong stability. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the present invention or the technical solutions in the prior art, the following will be described in a clear and understandable manner in conjunction with the accompanying drawings. Obviously, the drawings described below are only for the present invention, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0014] Figure 1 The internal structure of the mixed colloid of the invention is a scanning electron microscope image; Figure 2 This is a graph showing the results of the blood lipid content in the rat heart blood described in Experimental Example 1 of the present invention; Figure 3 This is the result diagram of the rat organ index described in Experimental Example 1 of the present invention; Figure 4 This is a diagram showing the light transmittance results described in Experimental Example 3 of the present invention. DETAILED DESCRIPTION

[0015] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments; based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0016] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0017] The experimental methods in the following examples are conventional methods unless otherwise specified; the experimental materials and test strains used in the following examples are purchased from commercial channels unless otherwise specified.

[0018] Example 1: This example provides a lipid-lowering camellia diester edible oil and a preparation method thereof. The lipid-lowering camellia diester edible oil comprises the following components in parts by weight: 90 parts of nutritious camellia diester oil mixture, 30 parts of nanoparticles that assist in lowering blood lipids and promoting metabolism, 7 parts of bamboo leaf antioxidants, and 4 parts of disodium edetate.

[0019] The raw materials for preparing the nutritious camellia diester oil mixture include the following components in parts by weight: 10 parts of walnuts, 7 parts of peas, 6 parts of sweet corn kernels, 33 parts of rapeseed oil, 42 parts of camellia diester oil, 37 parts of red pine seed oil, 39 parts of sunflower seed oil, and 7 parts of soluble soybean polysaccharides; the raw materials for preparing the auxiliary lipid-lowering and metabolism-promoting nanoparticles include the following components in parts by weight: 14 parts of cassia seeds, 16 parts of polygonum multiflorum, 4 parts of eucommia, 7 parts of dodder seeds, and 26 parts of sodium starch phosphate.

[0020] The method for preparing the nutritious camellia diester oil mixture specifically comprises the following steps: S1. Put walnuts, peas and sweet corn kernels into a pulping machine with a power of 1.5 kW, the pulping speed is 3000 r / min, the pulping temperature is 28°C, and the pulping time is 10 min. After pulping, put them into an enzymolysis tank with a power of 1.8 kW with cellulase and bromelain, the enzymolysis temperature is 50°C, the enzymolysis time is 2 h, the enzymolysis speed is 70 r / min, stir and enzymolyze to obtain a nutrient mixture; S2, the nutrient mixture prepared by S1 and rapeseed oil are placed in an ultrasonic extractor with a power of 1.5 kW, an ultrasonic frequency of 20 kHz, an ultrasonic extraction temperature of 40°C, and an ultrasonic extraction time of 30 min. After ultrasonic extraction, the mixture is placed in a high-speed centrifuge with a power of 1.6 kW, a centrifugal temperature of 2°C, a centrifugal speed of 22000 r / min, and a centrifugal time of 15 min. After centrifugation, the supernatant is taken to obtain nutrient rapeseed oil; S3, putting camellia diester oil, Korean pine seed oil and sunflower seed oil into a stirring tank with a power of 1.5 kW, stirring at a temperature of 25°C, a stirring speed of 130 r / min, a stirring time of 15 min, stirring and mixing to obtain a mixed edible oil; S4. Put the nutritious rapeseed oil prepared by S2, soluble soybean polysaccharide and the mixed edible oil prepared by S3 into a high-speed shear disperser with a power of 1.5 kW. The high-speed shear dispersion temperature is 23°C, the high-speed shear dispersion time is 12 min, and the high-speed shear dispersion speed is 3000 r / min. High-speed shear dispersion is performed to obtain a nutritious camellia diester oil mixture.

[0021] The preparation method of the auxiliary blood lipid lowering and metabolic promoting agent specifically comprises the following steps: L1. Put Cassia seed, Polygonum multiflorum, Eucommia ulmoides and Cuscuta seed into a pulverizer with a power of 1.4 kW, the pulverization temperature is 25°C, the pulverization time is 30 min, and the pulverization speed is 1200 r / min. After pulverization, put them into an ultrasonic extractor with a power of 1.5 kW with a 70% ethanol solution, the ultrasonic frequency is 20 kHz, the ultrasonic extraction temperature is 40°C, and the ultrasonic extraction time is 30 min. After ultrasonic extraction, a plant extract is obtained; L2, put the plant extract prepared in L1 and sodium starch phosphate into a stirring tank with a power of 1.2 kW, the stirring temperature is 50°C, the stirring speed is 120 r / min, the stirring time is 18 min, after stirring and mixing, place at a temperature of 25°C for 1 h to obtain a mixed colloid; L3. Place the mixed colloid prepared in L2 into a vacuum freeze-nano spray dryer with a power of 2.3 kW, with a vacuum degree of -0.05 MPa, a freeze-nano spray drying temperature of -40°C, a freeze-nano spray drying particle size of 100 nm, and a freeze-nano spray drying time of 2.5 h to obtain nanoparticles that assist in lowering blood lipids and promoting metabolism.

[0022] In step L1, the weight ratio of the 70% ethanol solution to Cassia seed is 0.9:1.

[0023] This embodiment also provides a method for preparing camellia diester edible oil that assists in lowering blood lipids, which specifically comprises the following steps: Step 1: Put the bamboo leaf antioxidant and disodium ethylenediaminetetraacetic acid into a stirring tank with a power of 1.2 kW, stir at a temperature of 20° C., stir at a speed of 150 r / min, and stir for 8 min to obtain an antioxidant stabilizing composition; Step 2: Add the lipid-lowering and metabolism-promoting nanoparticles into the antioxidant and stabilizing composition prepared in step 1, stir at a temperature of 20° C., a stirring speed of 150 r / min, and a stirring time of 10 min to obtain a mixed preparation; Step 3: Disperse the mixed preparation prepared in step 2 in the nutritious camellia diester oil mixture, put it into an ultrasonic agitator with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min to obtain camellia diester edible oil that assists in lowering blood lipids.

[0024] Example 2: This example provides a lipid-lowering camellia diester edible oil and a preparation method thereof. The lipid-lowering camellia diester edible oil comprises the following components in parts by weight: 100 parts of nutritious camellia diester oil mixture, 38 parts of nanoparticles that assist in lowering blood lipids and promoting metabolism, 9 parts of bamboo leaf antioxidants, and 7 parts of disodium edetate.

[0025] The raw materials for preparing the nutritious camellia diester oil mixture include the following components in parts by weight: 11 parts of walnuts, 9 parts of peas, 8 parts of sweet corn kernels, 36 parts of rapeseed oil, 48 parts of camellia diester oil, 41 parts of red pine seed oil, 42 parts of sunflower seed oil, and 10 parts of soluble soybean polysaccharides; the raw materials for preparing the auxiliary lipid-lowering and metabolism-promoting nanoparticles include the following components in parts by weight: 16 parts of cassia seeds, 18 parts of polygonum multiflorum, 6 parts of eucommia, 10 parts of dodder seeds, and 29 parts of sodium starch phosphate.

[0026] The method for preparing the nutritious camellia diester oil mixture specifically comprises the following steps: S1. Put walnuts, peas and sweet corn kernels into a pulping machine with a power of 1.6 kW, the pulping speed is 3000 r / min, the pulping temperature is 30°C, and the pulping time is 13 min. After pulping, put them into an enzymolysis tank with a power of 2.1 kW with cellulase and bromelain, the enzymolysis temperature is 52°C, the enzymolysis time is 2.3 h, the enzymolysis speed is 80 r / min, and the enzymolysis is stirred to obtain a nutrient mixture; S2, the nutrient mixture prepared by S1 and rapeseed oil are placed in an ultrasonic extractor with a power of 1.5 kW, an ultrasonic frequency of 20 kHz, an ultrasonic extraction temperature of 40°C, and an ultrasonic extraction time of 30 min. After ultrasonic extraction, the mixture is placed in a high-speed centrifuge with a power of 1.6 kW, a centrifugal temperature of 2°C, a centrifugal speed of 22000 r / min, and a centrifugal time of 15 min. After centrifugation, the supernatant is taken to obtain nutrient rapeseed oil; S3, putting camellia diester oil, Korean pine seed oil and sunflower seed oil into a stirring tank with a power of 1.6 kW, stirring at a temperature of 27° C., a stirring speed of 140 r / min, a stirring time of 20 min, stirring and mixing to obtain a mixed edible oil; S4. The nutritious rapeseed oil prepared in S2, the soluble soybean polysaccharide and the mixed edible oil prepared in S3 are placed in a high-speed shear disperser with a power of 1.6 kW. The high-speed shear dispersion temperature is 25°C, the high-speed shear dispersion time is 14 min, and the high-speed shear dispersion speed is 3000 r / min. High-speed shear dispersion is performed to obtain a nutritious camellia diester oil mixture.

[0027] The preparation method of the auxiliary blood lipid lowering and metabolic promoting agent specifically comprises the following steps: L1. Put Cassia seed, Polygonum multiflorum, Eucommia ulmoides and Cuscuta seed into a pulverizer with a power of 1.7 kW, the pulverization temperature is 26°C, the pulverization time is 35 min, and the pulverization speed is 1300 r / min. After pulverization, put them into an ultrasonic extractor with a power of 1.5 kW with a 70% ethanol solution, the ultrasonic frequency is 20 kHz, the ultrasonic extraction temperature is 40°C, and the ultrasonic extraction time is 30 min. After ultrasonic extraction, a plant extract is obtained; L2, put the plant extract prepared in L1 and sodium starch phosphate into a stirring tank with a power of 1.3 kW, the stirring temperature is 55°C, the stirring speed is 130 r / min, the stirring time is 22 min, after stirring and mixing, place at a temperature of 26°C for 1 h to obtain a mixed colloid; L3. Place the mixed colloid prepared in L2 into a vacuum freeze-nano spray dryer with a power of 2.5 kW, with a vacuum degree of -0.05 MPa, a freeze-nano spray drying temperature of -40°C, a freeze-nano spray drying particle size of 100 nm, and a freeze-nano spray drying time of 2.8 h to obtain nanoparticles that assist in lowering blood lipids and promoting metabolism.

[0028] In step L1, the weight ratio of the 70% ethanol solution to Cassia seed is 1.1:1.

[0029] This embodiment also provides a method for preparing camellia diester edible oil that assists in lowering blood lipids, which specifically comprises the following steps: Step 1: Put the bamboo leaf antioxidant and disodium ethylenediaminetetraacetic acid into a stirring tank with a power of 1.3 kW, the stirring temperature is 23° C., the stirring speed is 160 r / min, the stirring time is 10 min, and the mixture is mixed to obtain an antioxidant stabilizing composition; Step 2: Add the lipid-lowering and metabolism-promoting nanoparticles into the antioxidant and stabilizing composition prepared in step 1, stir at a temperature of 22° C., a stirring speed of 170 r / min, and a stirring time of 12 min to obtain a mixed preparation; Step 3: Disperse the mixed preparation prepared in step 2 in the nutritious camellia diester oil mixture, put it into an ultrasonic agitator with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min to obtain camellia diester edible oil that assists in lowering blood lipids.

[0030] Example 3: This example provides a lipid-lowering camellia diester edible oil and a preparation method thereof. The lipid-lowering camellia diester edible oil comprises the following components in parts by weight: 110 parts of nutritious camellia diester oil mixture, 44 parts of blood lipid-lowering and metabolism-promoting nanoparticles, 11 parts of bamboo leaf antioxidants, and 8 parts of disodium ethylenediaminetetraacetate.

[0031] The raw materials for preparing the nutritious camellia diester oil mixture include the following components in parts by weight: 12 parts of walnuts, 11 parts of peas, 9 parts of sweet corn kernels, 38 parts of rapeseed oil, 53 parts of camellia diester oil, 46 parts of red pine seed oil, 48 parts of sunflower seed oil, and 12 parts of soluble soybean polysaccharides; the raw materials for preparing the auxiliary lipid-lowering and metabolism-promoting nanoparticles include the following components in parts by weight: 18 parts of cassia seeds, 19 parts of polygonum multiflorum, 9 parts of eucommia, 11 parts of dodder seeds, and 31 parts of sodium starch phosphate.

[0032] The method for preparing the nutritious camellia diester oil mixture specifically comprises the following steps: S1. Put walnuts, peas and sweet corn kernels into a pulping machine with a power of 1.8 kW, the pulping speed is 3000 r / min, the pulping temperature is 33°C, and the pulping time is 15 min. After pulping, put them into an enzymolysis tank with a power of 2.2 kW with cellulase and bromelain, the enzymolysis temperature is 55°C, the enzymolysis time is 2.5 h, the enzymolysis speed is 90 r / min, and the enzymolysis is stirred to obtain a nutrient mixture; S2, the nutrient mixture prepared by S1 and rapeseed oil are placed in an ultrasonic extractor with a power of 1.5 kW, an ultrasonic frequency of 20 kHz, an ultrasonic extraction temperature of 40°C, and an ultrasonic extraction time of 30 min. After ultrasonic extraction, the mixture is placed in a high-speed centrifuge with a power of 1.6 kW, a centrifugal temperature of 2°C, a centrifugal speed of 22000 r / min, and a centrifugal time of 15 min. After centrifugation, the supernatant is taken to obtain nutrient rapeseed oil; S3, putting camellia diester oil, Korean pine seed oil and sunflower seed oil into a stirring tank with a power of 1.8 kW, stirring at a temperature of 28°C, a stirring speed of 150 r / min, and a stirring time of 25 min, stirring and mixing to obtain a mixed edible oil; S4. Put the nutritious rapeseed oil prepared by S2, soluble soybean polysaccharide and the mixed edible oil prepared by S3 into a high-speed shear disperser with a power of 1.8 kW, the high-speed shear dispersion temperature is 28°C, the high-speed shear dispersion time is 16 min, the high-speed shear dispersion speed is 4000 r / min, and high-speed shear dispersion is performed to obtain a nutritious camellia diester oil mixture.

[0033] The preparation method of the auxiliary blood lipid lowering and metabolic promoting agent specifically comprises the following steps: L1. Put Cassia seed, Polygonum multiflorum, Eucommia ulmoides and Cuscuta seed into a pulverizer with a power of 1.8 kW, the pulverization temperature is 28°C, the pulverization time is 40 min, and the pulverization speed is 1500 r / min. After pulverization, put them into an ultrasonic extractor with a power of 1.5 kW with 70% ethanol solution, the ultrasonic frequency is 20 kHz, the ultrasonic extraction temperature is 40°C, and the ultrasonic extraction time is 30 min. After ultrasonic extraction, a plant extract is obtained; L2, put the plant extract prepared in L1 and sodium starch phosphate into a stirring tank with a power of 1.4 kW, the stirring temperature is 60°C, the stirring speed is 140 r / min, the stirring time is 23 min, after stirring and mixing, place at a temperature of 28°C for 1 h to obtain a mixed colloid; L3. Place the mixed colloid prepared in L2 into a vacuum freeze-nano spray dryer with a power of 2.8 kW, with a vacuum degree of -0.05 MPa, a freeze-nano spray drying temperature of -40°C, a freeze-nano spray drying particle size of 100 nm, and a freeze-nano spray drying time of 3 h to obtain nanoparticles that assist in lowering blood lipids and promoting metabolism.

[0034] In step L1, the weight ratio of the 70% ethanol solution to Cassia seed is 1.2:1.

[0035] This embodiment also provides a method for preparing camellia diester edible oil that assists in lowering blood lipids, which specifically comprises the following steps: Step 1: Put the bamboo leaf antioxidant and disodium ethylenediaminetetraacetic acid into a stirring tank with a power of 1.4 kW, stir at a temperature of 26° C., stir at a speed of 180 r / min, and stir for 12 min to obtain an antioxidant stabilizing composition; Step 2: Add the lipid-lowering and metabolism-promoting nanoparticles into the antioxidant and stabilizing composition prepared in step 1, stir at a temperature of 26° C., a stirring speed of 180 r / min, and a stirring time of 14 min to obtain a mixed preparation; Step 3: Disperse the mixed preparation prepared in step 2 in the nutritious camellia diester oil mixture, put it into an ultrasonic agitator with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring time of 10 min to obtain camellia diester edible oil that assists in lowering blood lipids.

[0036] Comparative Example 1: This comparative example provides a camellia diester edible oil and a preparation method thereof, which is different from Example 1 only in that the added nutrient camellia diester oil mixture does not contain a nutrient mixture, and the remaining components, component contents, and method steps are the same as those in Example 1.

[0037] Comparative Example 2: This comparative example provides a camellia diester edible oil and a preparation method thereof. The difference from Example 1 is that the added auxiliary lipid-lowering and metabolic promoter does not contain plant extracts, and the remaining components, component contents, and method steps are the same as those in Example 1.

[0038] Experimental Example 1: Test to determine the effect of lowering blood lipids and the effect on liver and kidney.

[0039] The test steps for determining the lipid-lowering efficacy and liver and kidney effects of the camellia diester edible oil prepared in Examples 1-3 of the present invention are as follows: (1) Sixty SD male rats, each weighing 220 g (purchased from Chengdu Dashuo Animal Co., Ltd.), were randomly divided into six groups of 10 rats. The rats were housed in separate cages, which were kept clean and dry at a temperature of 24°C and a humidity of 50%. The rats were fed with free food and water; (2) After 10 days of feeding, the rats in Example 1-3 groups were fed with 2.0 mL of the camellia diester edible oil prepared in Example 1-3 for assisting in lowering blood lipids, the rats in Comparative Example 1-2 groups were fed with 2.0 mL of the camellia diester edible oil prepared in Comparative Example 1-2, and the rats in the control group were fed with 2.0 mL of commercially available ordinary camellia oil (purchased from Zhongshan Yaofu Food Co., Ltd.) three times a day for 13 weeks. (3) After being fed for 13 weeks, the rats in each group were fasted for 24 h and killed by cervical dislocation. The rat heart blood was collected and placed in a water bath at 37°C for 5 min. The blood was centrifuged at 4000 r / min for 5 min. The supernatant was collected and the levels of triglyceride and low-density lipoprotein cholesterol in the serum were detected using an automatic biochemical analyzer and recorded. The obtained data were statistically analyzed using IBM SPSS Statistics 22.0 software, and graphics were drawn using Origin 2022. (4) Separate the liver and kidney of the rat, rinse them with 0.9% saline, dry the surface moisture with filter paper, and weigh them quickly. The organ index (%) = weight of a certain organ / rat body weight × 100%. An increase in the organ index indicates congestion, edema, or hyperplasia of the organ, while a decrease in the organ index indicates organ atrophy and other degenerative changes.

[0040] Result analysis: Figure 1 This is a scanning electron microscope image of the internal structure of the mixed colloid of the present invention. Figure 2 The result graph of the blood lipid content of the rat heart described in Experimental Example 1 of the present invention is shown in the figure. As shown in the figure, the rats in Example 1-3 groups were fed 2.0 mL of the auxiliary lipid-lowering camellia diester edible oil prepared in Example 1-3 three times a day. After continuous feeding for 13 weeks, the triglyceride content in the serum of the rats in Example 1-3 groups was 0.85 mmol / L, 0.81 mmol / L, and 0.79 mmol / L, respectively, and the low-density lipoprotein cholesterol content in the serum was 3.12 mmol / L, 2.82 mmol / L, and 2.55 mmol / L, respectively. The rats in the comparative example 1 group were fed 2.0 mL of the camellia diester edible oil prepared in comparative example 1, three times a day, and after continuous feeding for 13 weeks, the triglyceride content in the serum of the rats in the comparative example 1 group was 0.88 mmol / L, and the low-density lipoprotein cholesterol content in the serum was 3.16 mmol / L. The rats in the comparative example 2 group were fed 2.0 mL of the camellia diester edible oil prepared in comparative example 2. mL, fed 3 times a day, after 13 weeks of continuous feeding, the serum triglyceride content of rats in the control group 2 was 1.22 mmol / L, and the serum low-density lipoprotein cholesterol content was 3.35 mmol / L. The rats in the control group were fed 2.0 mL of commercially available ordinary camellia oil each time, fed 3 times a day, after 13 weeks of continuous feeding, the serum triglyceride content of rats in the control group was 1.51 mmol / L, and the serum low-density lipoprotein cholesterol content was 4.11 mmol / L. Figure 3The rat organ index result diagram of Experimental Example 1 of the present invention is shown in the figure. As shown in the figure, the rats in the Example 1-3 groups were fed 2.0 mL of the auxiliary lipid-lowering camellia diester edible oil prepared in Example 1-3 three times a day. After continuous feeding for 13 weeks, the rat liver index was 0.44%, 0.53%, and 0.55%, respectively, and the rat kidney index was 1.08%, 1.13%, and 1.15%, respectively. The rats in the comparative example 1 group were fed 2.0 mL of the camellia diester edible oil prepared in comparative example 1, three times a day, and after continuous feeding for 13 weeks, the rat liver index was 0.42%, and the rat kidney index was 1.07%. The rats in the comparative example 2 group were fed 2.0 mL of the camellia diester edible oil prepared in comparative example 2, three times a day, and after continuous feeding for 13 weeks, the rat liver index was 0.74%, and the rat kidney index was 1.38%. The rats in the control group were fed 2.0 mL of commercially available ordinary camellia oil each time. mL, fed 3 times a day, after 13 weeks of continuous feeding, the liver index of the rats in the control group was 0.81%, and the kidney index of the rats was 1.43%, indicating that the blood lipid-lowering camellia diester edible oil prepared by the present invention will not increase blood lipids after long-term consumption, and has the effect of repairing the liver and kidneys, and does not affect the normal metabolism of fat by the liver and kidneys.

[0041] Experimental Example 2: Nutrient content determination test.

[0042] The test steps for determining the nutritional content of the camellia diester edible oil for assisting in lowering blood lipids prepared in Examples 1-3 of the present invention are as follows: (1) The determination method of carotene content in each group of edible oils refers to the standard GB / T 5009.83-2016 Determination of carotene in food, the determination method of chlorophyll content refers to the standard NY / T 3082-2017 Determination of chlorophyll content in fruits, vegetables and their products, the determination method of phytosterol content refers to the standard GB / T 39995-2021 Determination of sterols, and the determination method of vitamin E content refers to the standard GB5009.82-2016 Determination of vitamins A, D and E in food; (2) 1 L of the camellia diester edible oil prepared in Examples 1-3 for assisting in lowering blood lipids was taken as Example 1-3 group, 1 L of the camellia diester edible oil prepared in Comparative Example 1-2 was taken as Comparative Example 1-2 group, and 1 L of commercially available ordinary camellia oil (purchased from Zhongshan Yaofu Food Co., Ltd.) was taken as the control group. Five parallel measurements were set for each group, and the average value was taken. The results are recorded in Table 1.

[0043] Table 1. Nutritional content of edible oils

[0044] Result analysis: As shown in Table 1, the carotene, chlorophyll, phytosterols and vitamin E contained in the camellia diester edible oil for assisting in lowering blood lipids prepared in Examples 1-3 are all higher than those in the camellia diester edible oil prepared in Comparative Example 1 and the commercially available ordinary camellia oil in the control group, indicating that the use of the nutrient mixture supplements the loss of nutrient components such as carotene, chlorophyll, phytosterols and vitamin E during the edible oil pressing process, and after high-speed shear dispersion, the nutrient rapeseed oil can be stably present in the mixed edible oil for a long time.

[0045] Experimental Example 3: Long-term storage stability test.

[0046] The long-term storage stability test steps of the camellia diester edible oil for assisting in lowering blood lipids prepared in Examples 1-3 of the present invention are as follows: (1) 100 mL of the camellia diester edible oil prepared in Examples 1-3 as samples of Example 1-3, 100 mL of the camellia diester edible oil prepared in Comparative Example 1-2 as samples of Comparative Example 1-2, and 100 mL of commercially available ordinary camellia oil (purchased from Zhongshan Yaofu Food Co., Ltd.) as samples of the control group, with 6 parallel samples in each group; (2) Store at 20°C in the dark. On the 120th day of storage, take out the edible oil samples from each group and evaluate their color, appearance, smell and taste according to the scoring criteria in Table 2. The average values ​​are recorded. The results are shown in Table 3. (3) Each group of edible oil samples were stored at 20°C in the dark. On the 20th, 40th, 60th, 80th, 100th, 120th, 140th and 160th days of storage, each group of samples was taken out and left for 10 min. The transmittance was measured using an LS116 transmittance meter and recorded.

[0047] Table 2. Edible oil sample scoring standard table

[0048] Table 3. Edible oil sample scoring table

[0049] Result analysis: Figure 4 The light transmittance result diagram of Experimental Example 3 of the present invention is shown in Table 3 and Figure 4As shown, the camellia diester edible oils for assisting in lowering blood lipids prepared in Examples 1-3 and the camellia diester edible oils prepared in Comparative Examples 1-2 were stored at 20°C in the dark, and at the 120th day of storage, the color, appearance, smell and taste scores were all higher than those of the commercially available ordinary camellia oil in the control group. In addition, the camellia diester edible oil for assisting in lowering blood lipids prepared in Example 1 was stored at 20°C in the dark, and at the 20th, 40th, 60th, 80th, 100th, 120th, 140th and 160th days of storage, the light transmittances of each group of samples taken out and placed for 10 min were 94.3%, 94.3%, 94.0%, 94.0%, 93.9%, 93.8%, 93.8% and 93.6%, respectively. The camellia diester edible oil prepared in Comparative Example 1 was stored at 20°C in the dark, and at the 20th, 40th, 60th, 80th, 100th, 120th, 140th and 160th days of storage, the light transmittances of each group of samples taken out and placed for 10 min were 94.3%, 94.3%, 94.0%, 94.0%, 93.9%, 93.8%, 93.8% and 93.6%, respectively. d, 100 d, 120 d, 140 d and 160 d, the samples in each group were taken out and placed for 10 min, and the transmittances measured were 93.8%, 93.6%, 93.4%, 93.2%, 93.2%, 93.0%, 92.9% and 92.9%, respectively. The camellia diester edible oil prepared in Comparative Example 2 was stored at 20°C in the dark. On the 20th, 40th, 60th, 80th, 100th, 120th, 140th and 160th d, the samples in each group were taken out and placed for 10 min, and the transmittances measured were 93.6%, 93.4%, 93.3%, 93.0%, 93.1%, 93.1%, 92.9% and 92.7%, respectively. The commercially available ordinary camellia oil in the control group was stored at 20°C in the dark. On the 20th, 40th, 60th, 80th, 100th, 120th, 140th and 160th d, the transmittances measured were 93.6%, 93.4%, 93.3%, 93.0%, 93.1%, 92.9% and 92.7%, respectively. At 40 d, 80 d, 100 d, 120 d, 140 d and 160 d, the transmittances of the samples in each group were taken out and placed for 10 min, and were respectively 86.4%, 84.7%, 82.3%, 81.6%, 78.9%, 76.3%, 72.4% and 69.7%, indicating that the camellia diester edible oil for assisting in lowering blood lipids prepared by the present invention has a unique aroma, good taste, is not prone to deterioration during long-term storage, has strong stability, does not produce precipitation, and has high clarity.

[0050] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0051] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A camellia diester edible oil for assisting in lowering blood lipids, characterized in that: The raw materials for preparing the lipid-lowering camellia diester edible oil specifically include the following components in parts by weight: 90-110 parts of nutritious camellia diester oil mixture, 30-44 parts of nanoparticles that assist in lowering blood lipids and promoting metabolism, 7-11 parts of bamboo leaf antioxidants, and 4-8 parts of disodium edetate; The raw materials for preparing the nutritious camellia diester oil mixture include the following components in parts by weight: 10-12 parts of walnuts, 7-11 parts of peas, 6-9 parts of sweet corn kernels, 33-38 parts of rapeseed oil, 42-53 parts of camellia diester oil, 37-46 parts of red pine seed oil, 39-48 parts of sunflower seed oil, and 7-12 parts of soluble soybean polysaccharides; The raw materials for preparing the blood lipid-lowering and metabolism-promoting nanoparticles include the following components in parts by weight: 14-18 parts of Cassia seeds, 16-19 parts of Polygonum multiflorum, 4-9 parts of Eucommia ulmoides, 7-11 parts of Cuscuta seeds, and 26-31 parts of sodium starch phosphate.

2. The camellia diester edible oil for assisting in lowering blood lipids according to claim 1, characterized in that: The method for preparing the nutritious camellia diester oil mixture specifically comprises the following steps: S1, grinding walnuts, peas and sweet corn kernels and performing enzymatic hydrolysis to obtain a nutrient mixture; S2, extracting the nutrient mixture prepared in S1 by ultrasonic wave with rapeseed oil, and then centrifuging and taking the supernatant to obtain nutrient rapeseed oil; S3, stirring and mixing camellia diester oil, Korean pine seed oil, and sunflower seed oil to obtain a mixed edible oil; S4, subjecting the nutritious rapeseed oil prepared in S2, the soluble soybean polysaccharide and the mixed edible oil prepared in S3 to high-speed shear dispersion to obtain a nutritious camellia diester oil mixture.

3. The camellia diester edible oil for assisting in lowering blood lipids according to claim 2, characterized in that: The method for preparing the nanoparticles that assist in lowering blood lipids and promoting metabolism specifically comprises the following steps: L1. Mix and grind Cassia seed, Polygonum multiflorum, Eucommia ulmoides and Cuscuta australis, and perform ultrasonic extraction using 70% ethanol solution to obtain a plant extract; L2, after stirring and mixing the plant extract prepared in L1 and sodium starch phosphate, leaving it at a temperature of 25-28°C for 1 hour to obtain a mixed colloid; L3. The mixed colloid prepared in L2 is freeze-nano spray-dried to obtain nanoparticles that assist in lowering blood lipids and promoting metabolism.

4. The camellia diester edible oil for assisting in lowering blood lipids according to claim 3, characterized in that: In step L1, the weight ratio of the 70% ethanol solution to Cassia seed is 0.9-1.2:

1.

5. A method for preparing the camellia diester edible oil for assisting in lowering blood lipids according to claims 1-4, characterized in that: The specific steps include: Step 1, mixing bamboo leaf antioxidants and disodium ethylenediaminetetraacetate to obtain an antioxidant stabilizing composition; Step 2: Place the lipid-lowering and metabolism-promoting nanoparticles into the antioxidant and stabilizing composition prepared in step 1, mix them evenly and then disperse them in the nutritious camellia diester oil mixture to obtain lipid-lowering camellia diester edible oil.

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