Medical sodium hyaluronate gel and preparation method thereof

Abstract

The invention provides medical sodium hyaluronate gel and a preparation method thereof. The preparation method comprises the following steps: uniformly stirring and mixing the water for injection, the composite sodium hyaluronate and the sodium chloride, cooling and filling to obtain the sodium hyaluronate gel; the preparation process of the composite sodium hyaluronate comprises the following steps: firstly, preparing cross-linked sodium hyaluronate by taking macromolecular sodium hyaluronate, middle-molecular sodium hyaluronate and chitosan oligosaccharide as raw materials; then preparing composite micromolecular sodium hyaluronate by taking micromolecular hyaluronic acid, water-soluble beta-glucan and carbomer as raw materials; and finally mixing to prepare the composite sodium hyaluronate. The sodium hyaluronate gel can be absorbed and utilized to different degrees, the action time effect is effectively prolonged, a stable cross-linked network can be formed, an appropriate microenvironment can be provided for non-chronic wound repair, and wound repair and healing are promoted.

Description

Technical Field

[0001] The present invention relates to the technical field of sodium hyaluronate, and in particular to a medical sodium hyaluronate gel and a preparation method thereof. Background Art

[0002] The skin is the outermost protective barrier of the human body. Due to the influence of environmental factors, it causes wounds such as abrasions and scratches, or wounds appear after micro-plastic surgery such as laser and microneedle therapy. If these non-chronic wounds are not cared for in time, they will cause infection, inflammation, suppuration, and difficulty in healing.

[0003] Sodium hyaluronate (HA), also known as hyaluronic acid, is an acidic straight-chain polysaccharide composed of repeated disaccharides of (1-3)-2-N-acetylamino-2-deoxy-D-glucose-(1-4)-O-β-D-glucuronic acid, and has strong hydrophilicity, moisturizing properties and biocompatibility. Sodium hyaluronate is widely present in the extracellular matrix of connective tissue of animals and humans. After entering the human body, sodium hyaluronate can be hydrolyzed into hyaluronic acid, which has the effects of moisturizing, soothing, anti-inflammatory, repairing, increasing skin elasticity, and anti-wrinkle. At present, although some nursing solutions for non-chronic wounds based on sodium hyaluronate have appeared on the market, the effect is not ideal, and it is not able to provide an ideal nursing environment for non-chronic wounds, and it is difficult to promote the healing of non-chronic wounds. Summary of the invention

[0004] In view of this, the present invention proposes a medical sodium hyaluronate gel and a preparation method thereof

[0005] The technical solution of the present invention is achieved in this way:

[0006] A method for preparing medical sodium hyaluronate gel comprises the following steps:

[0007] Weigh the water for injection and put it into a stirring tank, then add the weighed composite sodium hyaluronate and sodium chloride into the stirring tank, stir and mix, cool down to a temperature of ≤45°C while stirring, and then fill to obtain medical sodium hyaluronate gel;

[0008] The preparation process of the composite sodium hyaluronate comprises the following steps:

[0009] (1) dissolving macromolecular sodium hyaluronate and medium molecular sodium hyaluronate in a sodium hydroxide aqueous solution and stirring evenly, then adding chitosan oligosaccharide and a cross-linking agent to carry out stirring reaction, adjusting the pH to neutral to terminate the reaction, and preparing cross-linked sodium hyaluronate;

[0010] (2) Sprinkling carbomer into purified water to swell at room temperature; adding small molecule sodium hyaluronate and water-soluble β-glucan into the swollen carbomer while stirring to obtain composite small molecule sodium hyaluronate;

[0011] (3) The cross-linked sodium hyaluronate prepared in the above (1) and the composite small molecule sodium hyaluronate prepared in the above (2) are uniformly stirred to obtain composite sodium hyaluronate.

[0012] Furthermore, the mass ratio of the cross-linked sodium hyaluronate to the composite small molecule sodium hyaluronate is 1:2-4.

[0013] Furthermore, the stirring and mixing temperature is 75-85° C. and the stirring frequency is 15-20 Hz.

[0014] Furthermore, the filling temperature is 18-28° C. and the relative humidity is 45%-65%.

[0015] Furthermore, the molecular weight of the macromolecular sodium hyaluronate is 2 to 2.5 million Da, the molecular weight of the medium-molecular sodium hyaluronate is 1.2 to 1.6 million Da; and the molecular weight of the small-molecular sodium hyaluronate is 40,000 to 100,000 Da.

[0016] Furthermore, the mass fraction of the sodium hydroxide aqueous solution is 0.5% to 1.5%; and the cross-linking agent is 1,4-butanediol diglycidyl ether.

[0017] Furthermore, the mass ratio of the macromolecular sodium hyaluronate, the medium molecular sodium hyaluronate, the sodium hydroxide aqueous solution, the chitosan oligosaccharide, and the cross-linking agent is 10-18:12-16:80-120:4-8:1.

[0018] Furthermore, the g / mL ratio of the carbomer to water is 1:90-120; the mass ratio of the small molecule sodium hyaluronate to purified water is 1:9-15; the mass ratio of the small molecule sodium hyaluronate, water-soluble β-glucan, and carbomer is 20-30:10-15:14-18.

[0019] Furthermore, in step (2) and step (3), the stirring frequency is 10 to 15 Hz.

[0020] Furthermore, the carbomer is carbomer 940.

[0021] Medical sodium hyaluronate gel prepared by the preparation method of the invention.

[0022] Furthermore, the medical sodium hyaluronate gel comprises the following raw materials in parts by weight: 1 to 3 parts of composite sodium hyaluronate, 0.5 to 0.9 parts of sodium chloride, and 95 to 110 parts of water for injection.

[0023] Furthermore, the medical sodium hyaluronate gel is used in the preparation of medical sodium hyaluronate gel for the care of non-chronic wounds of the skin.

[0024] Furthermore, the non-chronic wounds include small wounds, abrasions, and wounds formed after laser / photon / acid peeling / micro-plastic surgery.

[0025] Furthermore, the medical sodium hyaluronate gel of the present invention is packaged in the following specifications: 2 ml / bottle, 3 ml / bottle, 5 ml / bottle, 8 ml / bottle, and 10 ml / bottle.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] In the process of preparing the sodium hyaluronate gel, the present invention cross-links and compounds the high molecular weight and medium molecular weight sodium hyaluronate with chito oligosaccharide, and then compounds the low molecular weight sodium hyaluronate with water-soluble β-glucan, which can form a moisturizing film on the surface of the non-chronic wound, maintain the humidity of the non-wound surface and prevent water loss, provide a suitable microenvironment for the repair of the non-chronic wound surface, help the movement of non-chronic wound surface cells and provide cell nutrition, promote the repair and healing of the wound surface, effectively shorten the wound healing time, and enable the prepared sodium hyaluronate gel to be absorbed and utilized to varying degrees, effectively prolonging the action time effect.

[0028] The present invention prepares composite sodium hyaluronate through a suitable process, which can improve the stability of sodium hyaluronate. During use, while maintaining a suitable non-chronic wound repair environment, the absorption time of sodium hyaluronate can be slowed down to improve the effect. In addition, the compounding of sodium hyaluronate with different molecular weights can play a synergistic role, thereby effectively shortening the wound healing time. DETAILED DESCRIPTION

[0029] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0030] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0031] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0032] Example 1

[0033] The preparation process of the composite sodium hyaluronate of this embodiment comprises the following steps:

[0034] (1) taking a macromolecular sodium hyaluronate with a molecular weight of 2 million Da and a medium molecular sodium hyaluronate with a molecular weight of 1.6 million Da, dissolving them in a sodium hydroxide aqueous solution with a mass fraction of 0.5%, stirring them evenly, then adding chitosan oligosaccharide and 1,4-butanediol diglycidyl ether crosslinking agent, stirring and reacting them at 40° C. for 5 hours, adjusting the pH to neutral to terminate the reaction, and preparing crosslinked sodium hyaluronate; the mass ratio of the macromolecular sodium hyaluronate, the medium molecular sodium hyaluronate, the sodium hydroxide aqueous solution, the chitosan oligosaccharide, and the crosslinking agent is 18:14:120:8:1;

[0035] (2) Sprinkling carbomer into purified water at a carbomer to water ratio of 1:100 g / mL and swelling at room temperature for 24 hours; adding small molecule sodium hyaluronate with a molecular weight of 60,000 Da and water-soluble β-glucan to the swollen carbomer while stirring at a stirring frequency of 15 Hz to obtain composite small molecule sodium hyaluronate; wherein the mass ratio of small molecule sodium hyaluronate, water-soluble β-glucan and carbomer is 20:15:16;

[0036] (3) The cross-linked sodium hyaluronate prepared in the above (1) and the composite small molecule sodium hyaluronate prepared in the above (2) are stirred uniformly at a stirring frequency of 15 Hz to obtain composite sodium hyaluronate; wherein the mass ratio of the cross-linked sodium hyaluronate to the composite small molecule sodium hyaluronate is 1:3.

[0037] The medical sodium hyaluronate gel of this embodiment includes the following raw materials in parts by weight: 2.3 parts of composite sodium hyaluronate, 0.7 parts of sodium chloride, and 97 parts of water for injection.

[0038] The preparation method of the medical sodium hyaluronate gel comprises the following steps:

[0039] Weigh the water for injection and put it into a stirring tank, then add the weighed composite sodium hyaluronate and sodium chloride into the stirring tank, stir and mix at a temperature of 80°C and a stirring frequency of 15 Hz until dissolved, cool down to a temperature ≤45°C while stirring, and then fill at a temperature of 25°C and a relative humidity of 45% to obtain medical sodium hyaluronate gel.

[0040] Example 2

[0041] The preparation process of the composite sodium hyaluronate of this embodiment comprises the following steps:

[0042] (1) taking a macromolecular sodium hyaluronate with a molecular weight of 2.5 million Da and a medium molecular sodium hyaluronate with a molecular weight of 1.4 million Da, dissolving them in a sodium hydroxide aqueous solution with a mass fraction of 1.5%, stirring them evenly, then adding chitosan oligosaccharide and 1,4-butanediol diglycidyl ether crosslinking agent, stirring and reacting them at 40° C. for 5 hours, adjusting the pH to neutral to terminate the reaction, and preparing crosslinked sodium hyaluronate; the mass ratio of the macromolecular sodium hyaluronate, the medium molecular sodium hyaluronate, the sodium hydroxide aqueous solution, the chitosan oligosaccharide, and the crosslinking agent is 10:16:100:4:1;

[0043] (2) Sprinkling carbomer into purified water at a carbomer to water g / mL ratio of 1:90 and swelling at room temperature for 24 hours; adding small molecule sodium hyaluronate with a molecular weight of 80,000 Da and water-soluble β-glucan to the swollen carbomer while stirring at a stirring frequency of 12 Hz to obtain composite small molecule sodium hyaluronate; wherein the mass ratio of small molecule sodium hyaluronate, water-soluble β-glucan and carbomer is 30:10:18;

[0044] (3) The cross-linked sodium hyaluronate prepared in the above (1) and the composite small molecule sodium hyaluronate prepared in the above (2) are stirred uniformly at a stirring frequency of 12 Hz to obtain composite sodium hyaluronate; the mass ratio of the cross-linked sodium hyaluronate to the composite small molecule sodium hyaluronate is 1:3.

[0045] The medical sodium hyaluronate gel of this embodiment includes the following raw materials in parts by weight: 2.2 parts of composite sodium hyaluronate, 0.8 parts of sodium chloride, and 97 parts of water for injection.

[0046] The preparation method of the medical sodium hyaluronate gel comprises the following steps:

[0047] Weigh the water for injection and put it into a stirring tank, then add the weighed composite sodium hyaluronate and sodium chloride into the stirring tank, stir and mix at a temperature of 85°C and a stirring frequency of 20 Hz until dissolved, cool down to a temperature of ≤45°C while stirring, and then fill at a temperature of 18°C ​​and a relative humidity of 65% to obtain medical sodium hyaluronate gel.

[0048] Example 3

[0049] The preparation process of the composite sodium hyaluronate of this embodiment comprises the following steps:

[0050] (1) taking a macromolecular sodium hyaluronate with a molecular weight of 2.3 million Da and a medium molecular sodium hyaluronate with a molecular weight of 1.2 million Da, dissolving them in a sodium hydroxide aqueous solution with a mass fraction of 1.2%, stirring them evenly, then adding chitosan oligosaccharide and 1,4-butanediol diglycidyl ether crosslinking agent, stirring and reacting at 40° C. for 5 hours, adjusting the pH to neutral to terminate the reaction, and preparing crosslinked sodium hyaluronate; the mass ratio of the macromolecular sodium hyaluronate, the medium molecular sodium hyaluronate, the sodium hydroxide aqueous solution, the chitosan oligosaccharide, and the crosslinking agent is 15:12:80:7:1;

[0051] (2) Sprinkling carbomer into purified water at a carbomer to water g / mL ratio of 1:110 and swelling at room temperature for 24 hours; adding small molecule sodium hyaluronate with a molecular weight of 40,000 Da and water-soluble β-glucan to the swollen carbomer while stirring at a stirring frequency of 10 Hz to obtain composite small molecule sodium hyaluronate; wherein the mass ratio of small molecule sodium hyaluronate, water-soluble β-glucan and carbomer is 24:13:14;

[0052] (3) The cross-linked sodium hyaluronate prepared in the above (1) and the composite small molecule sodium hyaluronate prepared in the above (2) are stirred uniformly at a stirring frequency of 10 Hz to obtain composite sodium hyaluronate; the mass ratio of the cross-linked sodium hyaluronate to the composite small molecule sodium hyaluronate is 1:3.

[0053] The medical sodium hyaluronate gel of this embodiment includes the following raw materials in parts by weight: 2.1 parts of composite sodium hyaluronate, 0.9 parts of sodium chloride, and 97 parts of water for injection.

[0054] The preparation method of the medical sodium hyaluronate gel comprises the following steps:

[0055] Weigh the water for injection and put it into a stirring tank, then add the weighed composite sodium hyaluronate and sodium chloride into the stirring tank, stir and mix at a temperature of 75°C and a stirring frequency of 20 Hz until dissolved, cool down to a temperature of ≤45°C while stirring, and then fill at a temperature of 28°C and a relative humidity of 50% to obtain medical sodium hyaluronate gel.

[0056] Comparative Example 1

[0057] Compared with the embodiment, this comparative example is as follows: the cross-linked sodium hyaluronate is replaced with single macromolecular sodium hyaluronate and medium molecular sodium hyaluronate components.

[0058] The preparation process of the composite sodium hyaluronate in this comparative example comprises the following steps:

[0059] (1) Sprinkling carbomer into purified water at a carbomer to water ratio of 1:100 g / mL and swelling at room temperature for 24 hours; adding small molecule sodium hyaluronate with a molecular weight of 60,000 Da and water-soluble β-glucan to the swollen carbomer while stirring at a stirring frequency of 15 Hz to obtain composite small molecule sodium hyaluronate; wherein the mass ratio of small molecule sodium hyaluronate, water-soluble β-glucan and carbomer is 20:15:16;

[0060] (2) taking a macromolecular sodium hyaluronate with a molecular weight of 2 million Da, a medium molecular hyaluronic acid with a molecular weight of 1.6 million Da, and the composite small molecule sodium hyaluronate prepared in the above (1) in a mass ratio of 9:7 and stirring them evenly at a stirring frequency of 15 Hz to obtain composite sodium hyaluronate; the mass ratio of the total mass of the macromolecular sodium hyaluronate and the medium molecular sodium hyaluronate to the composite small molecule sodium hyaluronate is 1:3.

[0061] Comparative Example 2

[0062] This comparative example is compared with the embodiment: the composite small molecule sodium hyaluronate is replaced with a single small molecule sodium hyaluronate component.

[0063] The preparation process of the composite sodium hyaluronate in this comparative example comprises the following steps:

[0064] (1) taking a macromolecular sodium hyaluronate with a molecular weight of 2 million Da and a medium molecular sodium hyaluronate with a molecular weight of 1.6 million Da, dissolving them in a sodium hydroxide aqueous solution with a mass fraction of 0.5%, stirring them evenly, then adding chitosan oligosaccharide and 1,4-butanediol diglycidyl ether crosslinking agent, stirring and reacting them at 40° C. for 5 hours, adjusting the pH to neutral to terminate the reaction, and preparing crosslinked sodium hyaluronate; the mass ratio of the macromolecular sodium hyaluronate, the medium molecular sodium hyaluronate, the sodium hydroxide aqueous solution, the chitosan oligosaccharide, and the crosslinking agent is 18:14:120:8:1;

[0065] (2) The cross-linked sodium hyaluronate prepared in the above (1) and the small molecule sodium hyaluronate with a molecular weight of 60,000 Da are stirred uniformly at a stirring frequency of 15 Hz to obtain composite sodium hyaluronate; the mass ratio of the cross-linked sodium hyaluronate to the small molecule sodium hyaluronate is 1:3.

[0066] Comparative Example 3

[0067] Compared with Example 1, this comparative example is as follows: the preparation process of composite sodium hyaluronate is changed, and the rest is the same as Example 1.

[0068] The preparation process of the composite sodium hyaluronate of this comparative example comprises the following steps: taking macromolecular sodium hyaluronate, medium molecular sodium hyaluronate and small molecular sodium hyaluronate, dissolving them in a sodium hydroxide aqueous solution with a mass fraction of 0.5% and stirring them evenly, then adding chito oligosaccharide, water-soluble β-glucan and 1,4-butanediol diglycidyl ether cross-linking agent for stirring reaction, adjusting the pH to neutral to terminate the reaction, swelling naturally, and then using a 95% mass fraction ethanol solution for precipitation, filtering, and obtaining composite sodium hyaluronate; the mass ratio of the macromolecular sodium hyaluronate, medium molecular sodium hyaluronate, small molecular sodium hyaluronate, sodium hydroxide aqueous solution, chito oligosaccharide, water-soluble β-glucan and cross-linking agent is 18:14:20:220:8:10:2.

[0069] Test situation

[0070] The performance of the medical sodium hyaluronate gels prepared in Examples 1-3 and Comparative Examples 1-3 was tested, and the test process and results are shown below.

[0071] (1) Moisturizing

[0072] Weigh 0.5 g of the medical sodium hyaluronate gel prepared in Examples 1-3 and the comparative example respectively, and then evenly apply them on a glass plate with a specification of 5 cm×5 cm and a microporous ventilation tape (weigh the glass plate before applying), and then put the glass plate into a constant temperature and humidity dryer, weigh the mass before placement and after placement for 8 hours, and calculate the moisturizing rate.

[0073] Moisture retention rate (%) = (m2-m0) / (m1-m0)×100%

[0074] Wherein, m0 is the mass of the glass plate before adding the sample / g, m1 is the mass of the glass plate after adding the sample / g, and m2 is the mass of the glass plate after drying for 8 hours / g. The test results are shown in Table 1 below.

[0075] Moisturizing rate (%) Example 1 89.32 Example 2 89.06 Example 3 88.97 Comparative Example 1 83.45 Comparative Example 2 85.27 Comparative Example 3 82.48

[0076] The above results show that compared with comparative examples 1-3, the medical sodium hyaluronate gel prepared by embodiments 1-3 of the present invention has a good moisturizing effect, and the moisturizing performance can be enhanced by cross-linking and compounding large molecular weight and medium molecular weight sodium hyaluronate with chitosan oligosaccharide and compounding small molecular weight sodium hyaluronate with water-soluble β-glucan.

[0077] (2) Wound care effect

[0078] 42 Kunming mice of SPF grade, half male and half female, weighing 20±g were used. After 7 days of adaptive feeding, the mice were anesthetized under sterile conditions, and the same parts of the left and right sides of the back of the mice were shaved. The shaved area was 2.0×2.0 cm. The right shaved area of ​​each mouse was scratched with a scalpel to form a right wound. The mice were randomly divided into 7 groups, 6 mice in each group, half male and half female. The mice in groups 1-7 corresponded to Examples 1-3 and Comparative Examples 1-3, respectively. Sodium hyaluronate gel was applied 6 hours after the wound was formed. The medical sodium hyaluronate gel prepared by Examples 1-3 and Comparative Examples 1-3 was applied to the right wound area of ​​the mice in each group of groups 1-7, and the dosage was 1 mg / cm 2 , apply once in the morning and evening, and at the same time apply the same amount of normal saline to the shaved area on the left side for 15 consecutive days. Observe the condition of the mice and the time of scab formation and scab removal on the wound every day, and take the average value of the results. The results are shown in Table 2 below.

[0079] Table 2 Wound care experimental effect

[0080]

[0081]

[0082] The above results show that the sodium hyaluronate gel prepared by the embodiments 1-3 of the present invention can play an effective nursing role, effectively promote wound repair and healing, effectively shorten the wound healing time, and through a suitable preparation process, the various components in the sodium hyaluronate gel can play a synergistic role. Compared with Example 1, when preparing composite sodium hyaluronate, the cross-linked sodium hyaluronate is replaced with a single macromolecular sodium hyaluronate and a medium-molecular sodium hyaluronate component, and it is not cross-linked with chitosan oligosaccharide, and when preparing composite sodium hyaluronate, the composite small molecule sodium hyaluronate is replaced with a single small molecule sodium hyaluronate component, resulting in the weakening of the synergistic effect of each component in the sodium hyaluronate gel, so that the wound scab healing time is prolonged. Compared with Example 1, the preparation method of Comparative Example 3 is not suitable, resulting in poor stability of the composite sodium hyaluronate obtained, thereby affecting its effect. It is further explained that the sodium hyaluronate gel prepared by the present invention can form a stable cross-linked network on the wound surface, and the compounding of sodium hyaluronate with chito oligosaccharide and water-soluble β-glucan of different molecular weights can play a synergistic role, which can provide a suitable microenvironment for non-chronic wound repair and improve the nursing effect.

[0083] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for preparing medical sodium hyaluronate gel, characterized in that: The following steps are involved: Weigh the water for injection and put it into a stirring tank, then add the weighed composite sodium hyaluronate and sodium chloride into the stirring tank, stir and mix, cool down to a temperature of ≤45°C while stirring, and then fill to obtain medical sodium hyaluronate gel; The preparation process of the composite sodium hyaluronate comprises the following steps: (1) dissolving macromolecular sodium hyaluronate and medium molecular sodium hyaluronate in a sodium hydroxide aqueous solution and stirring evenly, then adding chitosan oligosaccharide and a cross-linking agent to carry out stirring reaction, adjusting the pH to neutral to terminate the reaction, and preparing cross-linked sodium hyaluronate; (2) Sprinkling carbomer into purified water to swell at room temperature; adding small molecule sodium hyaluronate and water-soluble β-glucan into the swollen carbomer while stirring to obtain composite small molecule sodium hyaluronate; (3) The cross-linked sodium hyaluronate prepared in the above (1) and the composite small molecule sodium hyaluronate prepared in the above (2) are uniformly stirred to obtain composite sodium hyaluronate.

2. The method for preparing medical sodium hyaluronate gel according to claim 1, characterized in that: The stirring and mixing temperature is 75-85° C., and the stirring frequency is 15-20 Hz; the filling temperature is 18-28° C., and the relative humidity is 45%-65%.

3. The method for preparing the medical sodium hyaluronate gel according to claim 1, characterized in that: The molecular weight of the macromolecular sodium hyaluronate is 2 to 2.5 million Da, the molecular weight of the medium-molecular sodium hyaluronate is 1.2 to 1.6 million Da; and the molecular weight of the small-molecular sodium hyaluronate is 40,000 to 100,000 Da.

4. The method for preparing the medical sodium hyaluronate gel according to claim 1, characterized in that: The mass fraction of the sodium hydroxide aqueous solution is 0.5% to 1.5%; and the cross-linking agent is 1,4-butanediol diglycidyl ether.

5. The method for preparing medical sodium hyaluronate gel according to claim 1, characterized in that: The mass ratio of the macromolecular sodium hyaluronate, the medium molecular sodium hyaluronate, the sodium hydroxide aqueous solution, the chitosan oligosaccharide and the cross-linking agent is 10-18:12-16:80-120:4-8:

1.

6. The method for preparing medical sodium hyaluronate gel according to claim 1, characterized in that: The g / mL ratio of the carbomer to water is 1:90-120; the mass ratio of the small molecule sodium hyaluronate, water-soluble β-glucan and carbomer is 20-30:10-15:14-18.

7. The method for preparing medical sodium hyaluronate gel according to claim 1, characterized in that: In step (2) and step (3), the stirring frequency is 10 to 15 Hz.

8. Medical sodium hyaluronate gel prepared by the preparation method according to any one of claims 1 to 7.

9. The medical sodium hyaluronate gel according to claim 8, characterized in that: The invention comprises the following raw materials in parts by weight: 1 to 3 parts of composite sodium hyaluronate, 0.5 to 1.5 parts of sodium chloride and 95 to 110 parts of water for injection.

10. The medical sodium hyaluronate gel according to any one of claims 8 to 9, characterized in that: The medical sodium hyaluronate gel is used in preparing the medical sodium hyaluronate gel for skin non-chronic wound care.

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