Micro-fluidic chip, chip array and desoxyribonucleic acid storage method
By designing a microfluidic chip that integrates mineralization, inkjet printing, demineralization and amplification components, the lack of synthesis, storage, and amplification modules in DNA data storage is solved, and efficient integration and cost of DNA data storage is achieved. reduce.
Patent Information
- Application Number
- CN202411277450.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2024-06-30
- Filing Date
- 2024-09-12
- Publication Date
- 2025-01-10
AI Technical Summary
The existing DNA data storage technology is carried out in modules, which is cumbersome and time-consuming, and lacks modules that integrate synthesis, storage, amplification and indexing in DNA data storage, resulting in high synthesis and sequencing costs.
A microfluidic chip is designed to integrate mineralization components, inkjet printing components, demineralization components and amplification components. Through fluid experiments and calculations, the synthesis, storage and amplification functions of DNA data storage are realized. And the DNA storage samples were obtained by centrifugation and rotation through the chip array.
The integration of the synthesis, storage and amplification functions of DNA data storage is realized, which reduces costs, improves the repeatability and portability of the chip, and verifies the feasibility of DNA data storage.
Smart Images
Figure CN119281412A_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of data storage, and in particular to a microfluidic chip, a chip array and a deoxyribonucleic acid storage method. Background Art
[0002] Existing data storage is mainly based on silicon. Commonly used storage media such as solid-state hard drives and optical fibers use magnetism and light as the medium, but this type of data storage has low storage density and short storage time. The problem caused by low storage density is that it occupies a large area. Now the area of storage base stations has become larger and larger, constantly squeezing the living space of cities and rural areas. It has attracted the attention of the country and is considering new storage solutions; the storage time of existing storage primitives is 5-10 years or even shorter, and it is difficult to store extremely important data, such as national chronicles, history of philosophy, and history of science; DNA has become an important medium to replace existing storage primitives due to its long storage time and high storage density. DNA can store data for more than 20,000 years. Taking fossils as an example, even DNA extracted from fossils in ancient times can restore the genetic information in them; from the perspective of density, 1gDNA can store all the data on this planet;
[0003] However, DNA data storage is still carried out in modules, and each step is cumbersome and time-consuming. Microfluidic chips are well-known for their precise fluid control and powerful integration performance. There are still no reports on the integration of synthesis, storage, amplification, and indexing modules in DNA data storage with microfluidic chips. Therefore, this patent aims to solve this fundamental problem, integrate the modules of DNA data storage and reduce the cost of synthesis and sequencing.
[0004] The existing implementation scheme that is most similar to the present invention is an article published by a doctoral student of Jiang Xingyu's research group at the Southern University of Science and Technology, titled "A nanoparticle-coated microfluidic chip for automated, non-destructive extraction of encapsu lated DNA in data storaget", which integrates the storage and amplification modules in DNA data storage. Summary of the invention
[0005] The main purpose of the embodiments of the present application is to propose a microfluidic chip, a chip array and a method for storing deoxyribonucleic acid, aiming to provide a microfluidic chip that can integrate the synthesis, storage and amplification functions of DNA data storage and a method for reading DNA using a microfluidic chip.
[0006] To achieve the above-mentioned purpose, a first aspect of an embodiment of the present application proposes a microfluidic chip monomer, wherein the microfluidic chip monomer comprises a chip body, wherein the chip body has a rotation center that rotates under centrifugal action, and wherein the chip body is provided with:
[0007] A mineralization component; the mineralization component comprises a first mineralization liquid storage chamber, a second mineralization liquid storage chamber, a barrier pipe and a curved pipe, the first mineralization liquid storage chamber is used to store calcium chloride, the second mineralization liquid storage chamber is used to store sodium carbonate, the first mineralization liquid storage chamber is connected to the second mineralization liquid storage chamber through the barrier pipe, and the barrier pipe is connected to the curved pipe in a direction away from the rotation center;
[0008] An inkjet printing assembly; the inkjet printing assembly comprises a conical conduit, a vertical conduit and a thin conduit connected in sequence in a direction away from the rotation center, the conical conduit has a narrow opening away from the thin conduit, and the conical conduit is connected to the curved pipe through the narrow opening in a direction close to the rotation center;
[0009] A demineralization component; the demineralization component comprises a demineralization liquid storage chamber and a demineralization catheter connected to the demineralization liquid storage chamber, the demineralization liquid storage chamber is used to store EDTA heparin sodium, and the demineralization catheter is connected to the vertical catheter in a direction away from the rotation center;
[0010] an amplification component; the amplification component comprises an amplification buffer storage chamber, an intermediate conduit, a deoxyribonucleic acid amplification storage chamber and an amplification conduit which are connected in sequence in a direction away from the rotation center, and the amplification conduit is connected to the vertical conduit in a direction away from the rotation center;
[0011] and,
[0012] A liquid storage tank assembly; the liquid storage tank assembly is connected to the thin catheter in a direction close to the rotation center, and the liquid storage tank assembly includes a liquid storage tank body, which is used to store liquid flowing out of the inkjet printing assembly.
[0013] To achieve the above objectives, the second aspect of an embodiment of the present application proposes a chip array, comprising a protective shell and a microfluidic chip unit, wherein the protective shell is provided with fixing holes for fixing the chip array on a centrifuge, and the protective shell wraps the microfluidic chip unit.
[0014] To achieve the above-mentioned purpose, a third aspect of the embodiments of the present application provides a method for storing deoxyribonucleic acid, the method comprising:
[0015] Get test DNA;
[0016] A fluid test is performed on the chip array to obtain a fluid test result, wherein the fluid test result indicates that the microfluidic chip monomer in the chip array can flow liquid or cannot flow liquid, and the microfluidic chip monomer can flow liquid including: when the rotation speed is 0 rad / s, no liquid flows in the cavity of the microfluidic chip monomer; when the rotation speed is increased to 350 rad / s, the demineralization reagent in the demineralization storage chamber can flow into the vertical conduit, and the amplification reagent in the amplification buffer storage chamber can flow into the deoxyribonucleic acid amplification storage chamber; when the rotation speed is increased to 500 rad / s, the amplification reagent in the deoxyribonucleic acid amplification storage chamber can flow into the vertical conduit; when the rotation speed is increased to 600 rad / s, the mineralization reagent in the first mineralization storage chamber and the second mineralization storage chamber can flow into the vertical conduit 122;
[0017] Based on the test deoxyribonucleic acid, a mineralization test is performed on the mineralized component in the microfluidic chip monomer to obtain a mineralization test result, wherein the mineralization test result indicates that the mineralized component can protect the test deoxyribonucleic acid or cannot protect the test deoxyribonucleic acid, and the mineralized component can protect the test deoxyribonucleic acid including being able to form a mineralized layer on the surface of the test deoxyribonucleic acid;
[0018] Based on the test deoxyribonucleic acid, a demineralization test is performed on the demineralization component in the microfluidic chip monomer to obtain a demineralization test result, wherein the mineralization test result indicates that the demineralization component can demineralize the test deoxyribonucleic acid or cannot demineralize the test deoxyribonucleic acid, and the fact that the demineralization component can demineralize the test deoxyribonucleic acid indicates that the mineralized layer can be removed from the surface of the test deoxyribonucleic acid;
[0019] If the fluid test result indicates that the microfluidic chip monomers in the chip array can flow liquid, the mineralization test result indicates that the mineralization component can protect the deoxyribonucleic acid, and the mineralization test result indicates that the demineralization component can demineralize the deoxyribonucleic acid, the chip array is used as a target chip array;
[0020] obtaining a target deoxyribonucleic acid, wherein the target deoxyribonucleic acid is fixed on a modified substrate;
[0021] Inlaying the modified substrate carrying the target deoxyribonucleic acid on the inkjet printing component in the target chip array, and protecting the deoxyribonucleic acid on the target substrate by a polypropylene encapsulation protection method to obtain mineralized deoxyribonucleic acid;
[0022] The target chip array is fixed on a centrifuge through a fixing hole, and the target chip array is centrifugally rotated by the centrifuge to obtain a deoxyribonucleic acid storage sample.
[0023] The microfluidic chip, chip array and DNA storage method proposed in this application are designed to integrate the synthesis, storage and amplification modules in DNA data storage. The performance of the microfluidic chip is realized through fluid experiments and calculations, and all reactions in the chip are obtained outside the chip to obtain preliminary results and optimize the reaction system. DNA synthesis is realized on the substrate, and the chip manufacturing scheme is optimized to make it compatible with DNA synthesis; after all this is achieved, all reaction reagents are proportionally reduced and placed in the chip, and all schemes for DNA data storage are realized in the chip, and the final results are characterized, including concentration and sequencing, to verify the feasibility of this scheme. BRIEF DESCRIPTION OF THE DRAWINGS
[0024] Figure 1 is a schematic diagram of the structure of a microfluidic chip monomer provided in an embodiment of the present application;
[0025] Figure 2 is a schematic diagram of the structure of a chip array provided in an embodiment of the present application;
[0026] Figure 3 is a flow chart of the deoxyribonucleic acid storage method provided in an embodiment of the present application;
[0027] Figure 4 Schematic diagrams of the chip array provided in the embodiments of the present application in different states; (a) is static, (b) is a rotation speed of 350 rad / s; (c) is a rotation speed of 500 rad / s; (d) is an internal schematic diagram at a rotation speed of 600 rad / s;
[0028] Figure 5 It is a flow chart of mineralization test and demineralization test provided in the embodiment of the present application;
[0029] Figure 6 is a flow chart of DNA synthesis provided in the embodiments of the present application;
[0030] Figure 7 This is a flow chart of DNA data amplification provided in the embodiment of the present application.
[0031] Figure 8 This is an experimental data diagram of using a chip array to store deoxyribonucleic acid provided in an embodiment of the present application. DETAILED DESCRIPTION
[0032] In order to make the purpose, technical solution and advantages of the present application more clearly understood, the present application is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.
[0033] It should be noted that, although the functional modules are divided in the device schematic diagram and the logical order is shown in the flowchart, in some cases, the steps shown or described may be performed in a different order than the module division in the device or the order in the flowchart. The terms "first", "second", etc. in the specification, claims and the above drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence.
[0034] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used herein are only for the purpose of describing the embodiments of this application and are not intended to limit this application.
[0035] Existing data storage is mainly based on silicon. Commonly used storage media such as solid-state hard drives and optical fibers use magnetism and light as the medium, but this type of data storage has low storage density and short storage time. The problem caused by low storage density is that it occupies a large area. Now the area of storage base stations has become larger and larger, constantly squeezing the living space of cities and rural areas. It has attracted the attention of the country and is considering new storage solutions; the storage time of existing storage primitives is 5-10 years or even shorter, and it is difficult to store extremely important data, such as national chronicles, history of philosophy, and history of science; DNA has become an important medium to replace existing storage primitives due to its long storage time and high storage density. DNA can store data for more than 20,000 years. Taking fossils as an example, even DNA extracted from fossils in ancient times can restore the genetic information in them; from the perspective of density, 1gDNA can store all the data on this planet;
[0036] However, DNA data storage is still carried out in modules, and each step is cumbersome and time-consuming. Microfluidic chips are well-known for their precise fluid control and powerful integration performance. There are still no reports on the integration of synthesis, storage, amplification, and indexing modules in DNA data storage with microfluidic chips. Therefore, this patent aims to solve this fundamental problem, integrate the modules of DNA data storage and reduce the cost of synthesis and sequencing.
[0037] The existing implementation scheme that is most similar to the present invention is an article published by a doctoral student of Jiang Xingyu's research group at Southern University of Science and Technology, titled "A nanoparticle-coated microfluidic chip for automated, non-destructive extraction of encapsulated DNA in data storage", which integrates the storage and amplification modules in DNA data storage.
[0038] Based on this, the embodiments of the present application provide a microfluidic chip, a chip array and a method for storing deoxyribonucleic acid, aiming to provide a microfluidic chip that can integrate the synthesis, storage and amplification functions of DNA data storage and a method for reading DNA using a microfluidic chip.
[0039] The microfluidic chip, chip array and DNA storage method provided in the embodiments of the present application are specifically described through the following embodiments. First, the microfluidic chip in the embodiments of the present application is described.
[0040] Figure 1 is a schematic diagram of the structure of a microfluidic chip monomer 100 provided in an embodiment of the present application, combined with Figure 1As shown, the microfluidic chip monomer 100 includes a chip body, the chip body has a rotation center 300 that rotates under centrifugal action, and the chip body is provided with a mineralization component 110, an inkjet printing component 120, a demineralization component 130, an amplification component 140 and a liquid storage tank component 150. Among them, the mineralization component 110 includes a first mineralization liquid storage chamber 111, a second mineralization liquid storage chamber 112, a barrier pipe 113 and a curved pipe 114, the first mineralization liquid storage chamber 111 is used to store calcium chloride, the second mineralization liquid storage chamber 112 is used to store sodium carbonate, the first mineralization liquid storage chamber 111 and the second mineralization liquid storage chamber 112 are connected through the barrier pipe 113, and the barrier pipe 113 is connected with the curved pipe 114 in the direction away from the rotation center 300. The inkjet printing assembly 120 includes a conical conduit 123, a vertical conduit 122 and a thin conduit 121 connected in sequence in a direction away from the rotation center 300. The conical conduit 123 has a narrow opening away from the thin conduit 121, and the conical conduit 123 is connected to the curved pipe 114 through the narrow opening in a direction close to the rotation center 300. The demineralization assembly 130 includes a demineralization liquid storage chamber 131 and a demineralization conduit 132 connected to the demineralization liquid storage chamber 131. The demineralization liquid storage chamber 131 is used to store EDTA heparin sodium, and the demineralization conduit 132 is connected to the vertical conduit 122 in a direction away from the rotation center 300. The amplification assembly 140 includes an amplification buffer liquid storage chamber 141, an intermediate conduit 143, a deoxyribonucleic acid amplification liquid storage chamber 142 and an amplification conduit 144 connected in sequence in a direction away from the rotation center 300. The amplification conduit 144 is connected to the vertical conduit 122 in a direction away from the rotation center 300. The liquid storage tank assembly 150 is connected to the thin conduit 121 in a direction close to the rotation center 300 . The liquid storage tank assembly 150 includes a liquid storage tank body, which is used to store liquid flowing out of the inkjet printing assembly 120 .
[0041] It should be noted that the functions of synthesis, storage, amplification, etc. of DNA data storage can be integrated into the microfluidic chip monomer 100 .
[0042] The existing technology integrates storage and amplification modules, but does not integrate DNA synthesis modules, and all reactions are carried out in solution. The chip is a disposable tool and lacks repeatability. The present application inherits the DNA synthesis module and allows all reactions to be carried out on a substrate, increasing repeatability and portability. Further contributions are made to the module integration of DNA data storage and further reducing the cost of DNA data storage.
[0043] The best existing technology only integrates DNA mineralization, demineralization and amplification modules, but does not integrate DNA synthesis modules, which is not as comprehensive as this patent. In addition, all reactions in the existing technology are carried out in the liquid phase, which makes the chip a disposable tool, resulting in high costs. All reactions in this patent are carried out on a substrate, which greatly enhances the reusability of the chip and greatly reduces costs.
[0044] Figure 2 is a schematic diagram of the structure of the chip array provided in the embodiment of the present application, combined with Figure 2 As shown, the chip array includes a protective shell 200 and eight microfluidic chip monomers 100 , wherein the protective shell 200 is provided with fixing holes 210 for fixing the chip array on a centrifuge, and the protective shell 200 wraps the microfluidic chip monomers 100 .
[0045] It should be noted that the eight microfluidic chip monomers 100 in the chip array are completely identical, so the chip array can realize functions such as synthesis, storage, and amplification of DNA data storage.
[0046] Figure 3 is a flow chart of a DNA storage method provided in an embodiment of the present application, which can realize DNA storage using the above chip array. Figure 3 The method may include but is not limited to steps S301 to S310.
[0047] Step S301, obtaining test DNA;
[0048] Step S302, performing a fluid test on the chip array to obtain a fluid test result, wherein the fluid test result indicates whether the microfluidic chip monomer 100 in the chip array can flow liquid or cannot flow liquid;
[0049] Step S303, based on the test DNA, performing a mineralization test on the mineralized component 110 in the microfluidic chip monomer 100 to obtain a mineralization test result, wherein the mineralization test result indicates whether the mineralized component 110 can protect the test DNA or cannot protect the test DNA;
[0050] Step S304, based on the test DNA, performing a demineralization test on the demineralization component 130 in the microfluidic chip monomer 100 to obtain a demineralization test result, wherein the demineralization test result indicates that the demineralization component 130 can demineralize the test DNA or cannot demineralize the test DNA;
[0051] Step S305, if the fluid test result indicates that the microfluidic chip monomer 100 in the chip array can flow liquid, the mineralization test result indicates that the mineralization component 110 can protect DNA, and the mineralization test result indicates that the demineralization component 130 can demineralize DNA, then the chip array is used as a target chip array;
[0052] Step S306, obtaining initial nucleotides;
[0053] Step S307, modifying the initial nucleotide onto a preset substrate to obtain a modified substrate carrying the initial nucleotide;
[0054] Step S308, performing DNA synthesis on the initial nucleotides on the modified substrate to obtain target DNA, wherein the target DNA is fixed on the modified substrate;
[0055] Step S309, embedding the modified substrate carrying the target DNA in the vertical conduit 122 of the inkjet printing assembly 120 in the target chip array, and protecting the DNA on the target substrate by polypropylene encapsulation protection method to obtain mineralized DNA;
[0056] Step S310, fix the target chip array on the centrifuge through the fixing hole 210, and use the centrifuge to centrifuge the target chip array to obtain a DNA storage sample.
[0057] In some embodiments of the present application, by performing a fluid test on the chip array, a fluid test result indicating whether the microfluidic chip unit 100 in the chip array can flow liquid or not can be obtained.
[0058] For details, refer to Figure 4As shown in (a), (b), (c) and (d), (a) represents the state of the chip array when no fluid test is performed, (b) represents the state of the chip array when the fluid test is performed at a rotation speed of 350 rad / s, (c) represents the state of the chip array when the fluid test is performed at a rotation speed of 500 rad / s, and (d) represents the state of the chip array when the fluid test is performed at a rotation speed of 600 rad / s. Specifically, when the rotation speed is 0 rad / s, no liquid circulates in the cavities of the microfluidic chip monomer 100. When the rotation speed is increased to 350 rad / s, the demineralization reagent in the demineralization storage chamber 131 flows into the vertical conduit 122, and the amplification reagent in the amplification buffer storage chamber 141 flows into the deoxyribonucleic acid amplification storage chamber 142. When the rotation speed is increased to 500 rad / s, the amplification reagent in the deoxyribonucleic acid amplification storage chamber 142 flows into the vertical conduit 122. When the rotation speed is increased to 600 rad / s, the mineralization reagents in the first mineralization storage chamber 111 and the second mineralization storage chamber 112 flow into the vertical conduit 122.
[0059] In some embodiments of the present application, a mineralization test is performed on the mineralization component 110 in the microfluidic chip monomer 100 according to the test DNA, and a mineralization test result indicating that the mineralization component 110 can protect the test DNA or cannot protect the test DNA can be obtained. The mineralization component 110 can protect the test DNA including forming a mineralized layer on the surface of the test DNA.
[0060] In some embodiments of the present application, according to the test DNA, the demineralization component 130 in the microfluidic chip monomer 100 is subjected to a demineralization test, and a demineralization test result indicating that the demineralization component 130 can demineralize the test DNA or cannot demineralize the test DNA can be obtained. The demineralization component 130 can demineralize the test DNA, indicating that the mineralized layer can be removed from the surface of the test DNA.
[0061] For details, refer to Figure 5As shown, DNA with Cy5 on one end and biotin on the other end was attached to a glass slide and mineralized with calcium carbonate for protection. The mineralization method was characterized using DNaseI and a gel imager. In the third step, if there is no fluorescence, it means that the DNA was cut and the protection was not good. If there is fluorescence, it means that the DNA could not be cut and the protection was good. In the last step, if there is no fluorescence, it means that the DNA was cut and the demineralization effect was good. If there is fluorescence, it means that the DNA could not be cut and the demineralization effect was poor. Specifically, a test deoxyribonucleic acid for testing is obtained, wherein one end of the test deoxyribonucleic acid is connected to a fluorescent dye, and the other end of the test deoxyribonucleic acid is connected to biotin, and the end of the test deoxyribonucleic acid containing biotin is fixed on a preset glass slide according to a preset streptavidin to obtain a fixed deoxyribonucleic acid, and then the fluorescence intensity of the fixed deoxyribonucleic acid is measured using a gel imager to obtain a first fluorescence intensity, wherein the first fluorescence intensity indicates the presence or absence of fluorescence on the fixed deoxyribonucleic acid, and the fixed deoxyribonucleic acid is mineralized and protected by calcium carbonate to obtain a mineralized deoxyribonucleic acid, and the mineralized deoxyribonucleic acid is treated with a nuclease. The method comprises the steps of: cutting the mineralized cut deoxyribonucleic acid with an acid to obtain a mineralized cut deoxyribonucleic acid, measuring the fluorescence intensity of the mineralized cut deoxyribonucleic acid with a gel imager to obtain a second fluorescence intensity, wherein the second fluorescence intensity indicates the presence or absence of fluorescence on the mineralized cut deoxyribonucleic acid, demineralizing the mineralized cut deoxyribonucleic acid to obtain demineralized deoxyribonucleic acid, cutting the demineralized deoxyribonucleic acid with a nuclease to obtain demineralized cut deoxyribonucleic acid, and measuring the fluorescence intensity of the demineralized cut deoxyribonucleic acid with a gel imager to obtain a third fluorescence intensity, wherein the third fluorescence intensity indicates the presence or absence of fluorescence on the demineralized cut deoxyribonucleic acid.
[0062] In one embodiment of the present application, both the initial nucleotides and deoxyribonucleic acids can be used to store data. The initial nucleotides storing data are modified onto a pre-set substrate to obtain a modified substrate carrying the initial nucleotides, and then the initial nucleotides on the modified substrate are subjected to deoxyribonucleic acid synthesis to obtain the target deoxyribonucleic acid, wherein the target deoxyribonucleic acid is fixed on the modified substrate, and finally, the modified substrate carrying the target deoxyribonucleic acid is embedded in the vertical conduit 122 of the inkjet printing component 120 in the target chip array, and the deoxyribonucleic acid on the target substrate is protected by a polypropylene encapsulation protection method to obtain a mineralized deoxyribonucleic acid, thereby realizing the synthesis function of DNA data storage.
[0063] For details, refer to Figure 6As shown, DNA in situ synthesis is carried out on a substrate, wherein the material of the substrate is not limited. First, the initial nucleotides are modified onto the substrate, and DNA synthesis is performed on the initial nucleotides on the substrate using the phosphoramidite triester method. Finally, the synthesized substrate carrying the target deoxyribonucleic acid is embedded in the vertical conduit 122 of each microfluidic chip monomer 100 in the target chip array using a chimeric method, and the target deoxyribonucleic acid is protected using a polypropylene encapsulation protection method to facilitate the subsequent microfluidic chip assembly process. It should be noted that the present application does not limit the method for modifying the initial nucleotides onto the substrate.
[0064] Finally, the target chip array is fixed on the centrifuge through the fixing hole 210, and the target chip array can be centrifugally rotated by the centrifuge to obtain a DNA storage sample. Specifically, the conditions for obtaining the DNA storage sample are consistent with the conditions for fluid testing of the chip array, that is, when the rotation speed is 0 rad / s, no liquid flows in the cavity of each microfluidic chip monomer 100 in the target chip array. When the rotation speed is increased to 350 rad / s, the demineralization reagent in the demineralization storage chamber 131 flows into the vertical conduit 122, and the amplification reagent in the amplification buffer storage chamber 141 flows into the DNA amplification storage chamber 142. When the rotation speed is increased to 500 rad / s, the amplification reagent in the DNA amplification storage chamber 142 flows into the vertical conduit 122. When the rotation speed is increased to 600 rad / s, the mineralization reagents in the first mineralization storage chamber 111 and the second mineralization storage chamber 112 flow into the vertical conduit 122.
[0065] It should be noted that the reference Figure 7 As shown, in the embodiment of the present application, when the amplification component 140 is used to implement the amplification function of DNA data storage, the amplification method used is the RPA isothermal amplification method. The advantage of using this method is that it does not require heating and can be performed at room temperature. It is very suitable for use in microfluidic chips. Amplification on the substrate requires adding a repetitive sequence to separate the recognition sequence from the substrate, thereby increasing the reaction rate of substrate amplification.
[0066] refer to Figure 8 As shown, Figure 8 It shows that the chip array is used to perform a reading experiment of DNA data storage. The present invention has been proved to be feasible through experiments. All the above experiments have been completed in the chip. The DNA synthesis module is still being optimized. The concentration of the final collected liquid is measured to be about 0.2 ng / μL, and the sequence integrity is 100%. This solution is feasible.
[0067] The key points of this application are functional integration, cost reduction and increased repeatability; the points to be protected are the chip design, the experimental design and the ideas involved in this patent.
[0068] The terms "first", "second", "third", "fourth", etc. (if any) in the specification of the present application and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the data used in this way can be interchangeable where appropriate, so that the embodiments of the present application described herein can be implemented in an order other than those illustrated or described herein. In addition, the terms "including" and "having" and any of their variations are intended to cover non-exclusive inclusions, for example, a process, method, system, product or device comprising a series of steps or units is not necessarily limited to those steps or units clearly listed, but may include other steps or units that are not clearly listed or inherent to these processes, methods, products or devices.
[0069] It should be understood that in the present application, "at least one (item)" means one or more, and "plurality" means two or more. "And / or" is used to describe the association relationship of associated objects, indicating that three relationships may exist. For example, "A and / or B" can mean: only A exists, only B exists, and A and B exist at the same time, where A and B can be singular or plural. The character " / " generally indicates that the objects associated before and after are in an "or" relationship. "At least one of the following" or similar expressions refers to any combination of these items, including any combination of single or plural items. For example, at least one of a, b or c can mean: a, b, c, "a and b", "a and c", "b and c", or "a and b and c", where a, b, c can be single or multiple.
[0070] In the several embodiments provided in the present application, it should be understood that the disclosed devices and methods can be implemented in other ways. For example, the device embodiments described above are only schematic. For example, the division of the above units is only a logical function division. There may be other division methods in actual implementation, such as multiple units or components can be combined or integrated into another system, or some features can be ignored or not executed. The mutual coupling or direct coupling or communication connection shown or discussed can be through some interfaces, indirect coupling or communication connection of devices or units, which can be electrical, mechanical or other forms.
[0071] The units described above as separate components may or may not be physically separated, and the components shown as units may or may not be physical units, that is, they may be located in one place or distributed on multiple network units. Some or all of the units may be selected according to actual needs to achieve the purpose of the solution of this embodiment.
[0072] In addition, each functional unit in each embodiment of the present application may be integrated into one processing unit, or each unit may exist physically separately, or two or more units may be integrated into one unit. The above-mentioned integrated unit may be implemented in the form of hardware or in the form of software functional units.
[0073] If the integrated unit is implemented in the form of a software functional unit and sold or used as an independent product, it can be stored in a computer-readable storage medium. Based on this understanding, the technical solution of the present application is essentially or the part that contributes to the prior art or all or part of the technical solution can be embodied in the form of a software product, and the computer software product is stored in a storage medium, including multiple instructions to enable a computer device (which can be a personal computer, server, or network device, etc.) to execute all or part of the steps of the methods of various embodiments of the present application. The aforementioned storage medium includes: U disk, mobile hard disk, read-only memory (Read-Only Memory, referred to as ROM), random access memory (Random Access Memory, referred to as RAM), disk or optical disk and other media that can store programs.
[0074] The preferred embodiments of the present application are described above with reference to the accompanying drawings, but the scope of the rights of the present application is not limited thereto. Any modification, equivalent substitution and improvement made by a person skilled in the art without departing from the scope and essence of the present application should be within the scope of the rights of the present application.
Claims
1. A microfluidic chip monomer, characterized in that: The chip body comprises a chip body having a rotation center that rotates under centrifugal force, and the chip body is provided with: A mineralization component; the mineralization component comprises a first mineralization liquid storage chamber, a second mineralization liquid storage chamber, a barrier pipe and a curved pipe, the first mineralization liquid storage chamber is used to store calcium chloride, the second mineralization liquid storage chamber is used to store sodium carbonate, the first mineralization liquid storage chamber is connected to the second mineralization liquid storage chamber through the barrier pipe, and the barrier pipe is connected to the curved pipe in a direction away from the rotation center; An inkjet printing assembly; the inkjet printing assembly comprises a conical conduit, a vertical conduit and a thin conduit connected in sequence in a direction away from the rotation center, the conical conduit has a narrow opening away from the thin conduit, and the conical conduit is connected to the curved pipe through the narrow opening in a direction close to the rotation center; A demineralization component; the demineralization component comprises a demineralization liquid storage chamber and a demineralization catheter connected to the demineralization liquid storage chamber, the demineralization liquid storage chamber is used to store EDTA heparin sodium, and the demineralization catheter is connected to the vertical catheter in a direction away from the rotation center; an amplification component; the amplification component comprises an amplification buffer storage chamber, an intermediate conduit, a deoxyribonucleic acid amplification storage chamber and an amplification conduit which are connected in sequence in a direction away from the rotation center, and the amplification conduit is connected to the vertical conduit in a direction away from the rotation center; and, A liquid storage tank assembly; the liquid storage tank assembly is connected to the thin catheter in a direction close to the rotation center, and the liquid storage tank assembly includes a liquid storage tank body, which is used to store liquid flowing out of the inkjet printing assembly.
2. A chip array, comprising a protective shell and the microfluidic chip monomer as claimed in claim 1, wherein the protective shell is provided with fixing holes for fixing the chip array on a centrifuge, and the protective shell wraps the microfluidic chip monomer.
3. A method for storing deoxyribonucleic acid, characterized in that: Using the chip array as claimed in claim 2 to store DNA, the method comprises: Get test DNA; A fluid test is performed on the chip array to obtain a fluid test result, wherein the fluid test result indicates that the microfluidic chip monomer in the chip array can flow liquid or cannot flow liquid, and the microfluidic chip monomer can flow liquid including: when the rotation speed is 0 rad / s, no liquid flows in the cavity of the microfluidic chip monomer; when the rotation speed is increased to 350 rad / s, the demineralization reagent in the demineralization storage chamber can flow into the vertical conduit, and the amplification reagent in the amplification buffer storage chamber can flow into the deoxyribonucleic acid amplification storage chamber; when the rotation speed is increased to 500 rad / s, the amplification reagent in the deoxyribonucleic acid amplification storage chamber can flow into the vertical conduit; when the rotation speed is increased to 600 rad / s, the mineralization reagent in the first mineralization storage chamber and the second mineralization storage chamber can flow into the vertical conduit 122; Based on the test deoxyribonucleic acid, a mineralization test is performed on the mineralized component in the microfluidic chip monomer to obtain a mineralization test result, wherein the mineralization test result indicates that the mineralized component can protect the test deoxyribonucleic acid or cannot protect the test deoxyribonucleic acid, and the mineralized component can protect the test deoxyribonucleic acid including being able to form a mineralized layer on the surface of the test deoxyribonucleic acid; Based on the test deoxyribonucleic acid, a demineralization test is performed on the demineralization component in the microfluidic chip monomer to obtain a demineralization test result, wherein the mineralization test result indicates that the demineralization component can demineralize the test deoxyribonucleic acid or cannot demineralize the test deoxyribonucleic acid, and the fact that the demineralization component can demineralize the test deoxyribonucleic acid indicates that the mineralized layer can be removed from the surface of the test deoxyribonucleic acid; If the fluid test result indicates that the microfluidic chip monomers in the chip array can flow liquid, the mineralization test result indicates that the mineralization component can protect the deoxyribonucleic acid, and the mineralization test result indicates that the demineralization component can demineralize the deoxyribonucleic acid, the chip array is used as a target chip array; obtaining a target deoxyribonucleic acid, wherein the target deoxyribonucleic acid is fixed on a modified substrate; The modified substrate carrying the target deoxyribonucleic acid is embedded in the vertical conduit of the inkjet printing component in the target chip array, and the deoxyribonucleic acid on the target substrate is protected by a polypropylene encapsulation protection method to obtain mineralized deoxyribonucleic acid; The target chip array is fixed on a centrifuge through a fixing hole, and the target chip array is centrifugally rotated by the centrifuge to obtain a deoxyribonucleic acid storage sample.