Dendrobium herbal tea and preparation method thereof

Abstract

The invention discloses dendrobium herbal tea and a preparation method thereof, and belongs to the technical field of medicine and food processing.The dendrobium herbal tea is prepared from 3-4 parts of dendrobium officinale, 2-3 parts of radix puerariae, 2-3 parts of rhizoma polygonati, 1-2 parts of chrysanthemum, 1-2 parts of honeysuckle, 3-4 parts of poria cocos, 3-4 parts of semen coicis and 1-2 parts of liquorice through the processes of extraction, filtration, refining, concentration, blending, filling, sterilization and the like. The herba dendrobii herbal tea provided by the invention is formulated according to the theory of traditional Chinese medicine, can help people with fire excess from yin deficiency to improve the conditions of yin impairment and body fluid deficiency, dry mouth and polydipsia, deficiency-heat after illness and the like by nourishing yin, clearing heat, invigorating spleen and excreting dampness, does not cause diarrhea, stomachache and other uncomfortable symptoms caused by spleen and stomach function decline, and is especially suitable for body fluid insufficiency, yin deficiency and internal heat caused by diabetes mellitus.

Description

Technical Field

[0001] The invention belongs to the technical field of medicine and food processing, and in particular relates to a dendrobium herbal tea and a preparation method thereof. Background Art

[0002] According to traditional Chinese medicine, "getting angry" belongs to the category of heat syndrome. When the yin and yang of the human body are out of balance, internal heat syndrome occurs. Generally, "fire" can be divided into two categories: "real fire" and "virtual fire". False fire can be further divided into two types: Yin deficiency fire and Qi deficiency fire (Qi deficiency internal heat). Yin deficiency fire refers to the deficiency of yin fluid and the hyperactivity of virtual fire. When yin is deficient, yang is hyperactive and heat is generated, which turns into virtual fire. It is often manifested as hot flashes, night sweats, weight loss, dry mouth and throat, fever in the five hearts, restlessness, red tongue without coating, and weak pulse. The treatment should be based on the principle of promoting body fluid and nourishing blood, nourishing yin and reducing fire.

[0003] At present, the herbal tea sold in the market can clear away heat, detoxify, reduce internal heat, and act as a diuretic. However, due to its cold nature, excessive drinking will damage the spleen and stomach. If consumed for a long time, it will destroy the balance of intestinal microecology, reduce human immunity, and lead to many other diseases. Especially for people with diabetes and hyperlipidemia, who have a deficiency-heat constitution and damaged stomach yang, long-term drinking of herbal tea will damage the spleen and stomach function of the human body, leading to a decline in spleen and stomach function, aggravating the condition, and more likely to experience diarrhea, loose stools, abdominal pain, bloating and other discomfort symptoms. Summary of the invention

[0004] In order to overcome the problems existing in the background technology, the present invention provides a dendrobium herbal tea, which is formulated according to the theoretical formula of traditional Chinese medicine. It can help people with yin deficiency and hyperactivity of fire to improve conditions such as yin damage and fluid deficiency, dry mouth and thirst, and post-illness heat, without causing a decline in spleen and stomach function to cause diarrhea, abdominal pain, abdominal distension and other uncomfortable symptoms. It is especially suitable for insufficient body fluid and yin deficiency and internal heat caused by diabetes.

[0005] To achieve the above object, the present invention is implemented through the following technical solutions:

[0006] The invention discloses a dendrobium herbal tea, wherein the main components are as follows: 3-4 parts of dendrobium candidum, 2-3 parts of kudzu root, 2-3 parts of polygonatum, 1-2 parts of chrysanthemum, 1-2 parts of honeysuckle, 3-4 parts of tuckahoe, 3-4 parts of coix seeds and 1-2 parts of liquorice.

[0007] In the formula, Dendrobium candidum is slightly cold in nature, sweet in taste, and enters the stomach and kidney meridians. It can benefit the stomach and produce fluid, nourish yin and clear heat. It is used for yin damage and fluid deficiency, dry mouth and thirst, poor appetite and retching, deficiency heat after illness, and blurred vision; Pueraria root is cool in nature, sweet and spicy in taste, and enters the spleen and stomach meridians. It can relieve muscles and reduce fever, produce fluid, promote rash, raise yang and stop diarrhea. It is used for exogenous fever and headache, thirst, diabetes, heat dysentery, and diarrhea; Polygonatum sibiricum is flat in nature, sweet in taste, and enters the spleen, lung, and kidney meridians. It can nourish qi and yin, strengthen the spleen, moisten the lungs, and benefit the kidneys. It is used for spleen and stomach weakness, fatigue, dry mouth and poor appetite, lung deficiency and dry cough, insufficient essence and blood, and internal heat and diabetes; Honeysuckle is cold in nature, sweet in taste, and enters the lung, heart, and stomach meridians. It can clear away heat and detoxify, cool and disperse wind-heat, and is used for carbuncle, furuncle, throat paralysis, erysipelas, heat toxic bloody dysentery, wind-heat cold, and fever caused by febrile diseases; Chrysanthemum is slightly cold in nature, sweet and bitter in taste , it enters the lung and liver meridians, dispels wind and heat, soothes the liver and improves eyesight, and is used for wind-heat colds, headaches and dizziness, red and swollen eyes, and blurred vision; Poria is flat in nature, sweet and light in taste, and enters the heart, lung, spleen, and kidney meridians. It is diuretic and dampness-injecting, invigorates the spleen and calms the mind. It is used for edema, oliguria, phlegm and fluid, palpitations, spleen deficiency and poor appetite, loose stools and diarrhea, restlessness, palpitations and insomnia; Coix seed is cool in nature, sweet and light in taste, and enters the spleen, stomach, and lung meridians. It invigorates the spleen and dampness-injecting, eliminates numbness and stops diarrhea, clears heat and discharges pus, and is used for edema, dysuria, dampness and numbness, cramps, spleen deficiency diarrhea, lung abscess, and intestinal abscess; Licorice is flat in nature, sweet in taste, and enters the heart, lung, spleen, and stomach meridians. It invigorates the spleen and replenishes qi, clears away heat and detoxifies, eliminates phlegm and stops cough, and harmonizes various medicines. It is used for spleen and stomach weakness, fatigue, palpitations and shortness of breath, cough with excessive sputum, abdominal distension, carbuncle and sore, and relieves the toxicity and potency of drugs. The whole formula is compatible with nourishing yin, clearing heat, strengthening the spleen and draining dampness. It is used for symptoms such as yin damage and body fluid deficiency, dry mouth and thirst, and deficiency fever after illness.

[0008] The specific steps of preparing the above-mentioned dendrobium herbal tea are:

[0009] (1) Prepare materials according to the formula, and all raw materials are commercially available decoction pieces;

[0010] (2) taking each component in the formula, extracting it 2-3 times with purified water 15-20 times the weight of the medicinal material, each time for 30-60 minutes, combining the extracts, filtering, adding 0.01-0.02% pectinase, 0.01-0.02% amylase, and 0.02-0.04% protease according to the amount of the filtrate, keeping it at 50-55° C. for 30-60 minutes, concentrating it to a volume 2-3 times the weight of the medicinal material, and cooling it to room temperature;

[0011] (3) adding 1 to 2 times the volume of the concentrate by mass, 80% or more ethanol solution, so that the ethanol concentration in the solution reaches 40% or more, leaving it to stand for more than 12 hours, taking the supernatant, adding diatomaceous earth for fine filtration, recovering ethanol, and concentrating it to a volume of 2 to 3 times the mass of the medicinal material;

[0012] (4) Add auxiliary materials and purified water to adjust to the required volume, mix, fill, and sterilize to obtain the finished product.

[0013] Beneficial effects of the present invention:

[0014] (1) The prescription of the present invention is formulated based on the theory of traditional Chinese medicine. It can help people with yin deficiency and hyperactivity of fire to improve conditions such as yin damage and fluid deficiency, dry mouth and thirst, and post-illness heat, by nourishing yin and clearing heat, strengthening the spleen and removing dampness. It is especially suitable for people with insufficient body fluid and internal heat caused by yin deficiency due to diabetes.

[0015] (2) While ensuring efficacy, it has little effect on the spleen and stomach functions, and drinking it will not cause diarrhea, abdominal pain, bloating and other uncomfortable symptoms. DETAILED DESCRIPTION

[0016] Example 1 Extract Preparation

[0017] Purchase decoction pieces from the Chinese herbal medicine market, weigh 40g of Dendrobium candidum, 30g of Pueraria root, 30g of Polygonatum sibiricum, 20g of Chrysanthemum, 20g of Honeysuckle, 40g of Poria, 40g of Coix seeds, and 20g of Licorice, add 1800ml of purified water, soak for 30min, boil for 60min, filter to extract juice, add 1200ml of purified water to extract twice, boil for 30min each time, combine the extracts to get 2100ml, filter with 200-mesh nylon filter cloth, add purified water to make up to 2500ml, and process as follows:

[0018] (1) Take 250 ml of the extract and bottle it;

[0019] (2) Take 250 ml of the extract, add diatomaceous earth, and vacuum filter in a Buchner funnel with filter paper;

[0020] (3) Take 1000 ml of the extract, concentrate it to 200 ml, cool it to room temperature, add 200 ml of edible ethanol, let it stand overnight, add diatomaceous earth to filter, recover the ethanol, concentrate it to 100 ml, take 25 ml of it, add water to make up to 250 ml, and bottle it;

[0021] (4) Take 1000 ml of the extract, heat it to 55°C, add 0.1 ml of pectinase, 0.1 ml of amylase, and 0.2 ml of protease, keep it at 50-55°C for 60 min, boil it, cool it, add diatomaceous earth to filter it, take 250 ml of the filtrate and bottle it, and treat the rest with other methods;

[0022] (5) Take 50 ml of the concentrate obtained in treatment (3), add water to make the volume 500 ml, add 0.05 ml of pectinase, 0.05 ml of amylase, and 0.1 ml of protease, keep at 50-55°C for 60 min, boil, cool, filter with diatomaceous earth, and take 250 ml of the filtrate and bottle;

[0023] (6) Take 500 ml of the filtrate from treatment (4), concentrate it to 100 ml, cool it, add 100 ml of edible ethanol, let it stand overnight, filter it with filter paper, recover the ethanol, add water to make the volume to 500 ml, and take 250 ml of the filtrate and bottle it.

[0024] Observe the properties of the solution, take bottled samples, sterilize at 115℃ for 10min, observe after cooling, and the results are shown in Table 1.

[0025] Table 1 Dendrobium herbal tea extraction process experiment

[0026] Serial number Treatment Experimental observation (1) No processing after extraction Turbid and opaque; a large amount of precipitation after sterilization, and stratification after standing (2) After extraction, filter paper fine filtration The viscosity is too high, which blocks the filter paper and prevents normal filtration. (3) Ethanol precipitation The filtration speed in the latter stage slows down, the solution is clear, but has a colloidal opalescence; after sterilization, there is a small amount of precipitation, brown-yellow (4) Enzymatic hydrolysis The filtration speed is fast, the solution is clear and transparent; there is a small amount of precipitation after sterilization, which is brown (5) Ethanol precipitation + enzymatic hydrolysis The second filtration speed is faster, the solution is clear and transparent; after sterilization, it is clear and transparent, brown-yellow (6) Enzymatic hydrolysis + ethanol precipitation The filtration speed is fast, the solution is clear and transparent; after sterilization, it is clear, transparent, and brown

[0027] From the above experimental results, we can see that:

[0028] (1) After the dendrobium herbal tea is extracted with water, it should be filtered with filter paper, otherwise it will affect the sensory perception of the finished product;

[0029] (2) After the extraction of the Dendrobium herbal tea, adding a variety of enzymes for enzymolysis can increase the filtration rate and improve the sensory properties of the finished product;

[0030] (3) After the dendrobium herbal tea is extracted and concentrated with water, it is precipitated with ethanol. Judging from the color of the finished product after sterilization, some of the active ingredients may have changed;

[0031] (4) Dendrobium herbal tea is processed by water extraction, enzymatic hydrolysis, concentration, and ethanol precipitation. The process efficiency is high and the sensory experience of the finished product is good.

[0032] Example 2 Extract Preparation

[0033] Weigh 300g of Dendrobium candidum, 200g of Pueraria root, 200g of Polygonatum, 100g of Chrysanthemum, 100g of Honeysuckle, 300g of Poria, 300g of Coix seeds, and 100g of Licorice, add 15L of purified water, soak for 30min, boil for 60min, filter through 200-mesh filter cloth, add 10L of purified water to the residue, boil for 30min, combine the filtrate to obtain 16.5L, add 3ml of pectinase, 3ml of amylase, and 6ml of protease, keep warm at 50-55℃ for 30min, concentrate to 4L, cool to room temperature, add 4L of edible ethanol solution, let stand for 24h, take the supernatant, add 80g of diatomaceous earth, add a Buchner funnel covered with filter paper during stirring, vacuum filter, and store in a sealed container. 10L (8L) of ethanol extract is equivalent to 100 servings. Take an appropriate amount before use and recycle the ethanol for use.

[0034] Example 3 Extract Preparation

[0035] Weigh 300g of Dendrobium candidum, 225g of Pueraria root, 225g of Polygonatum sibiricum, 150g of Chrysanthemum, 150g of Honeysuckle, 300g of Poria, 300g of Coix seeds, and 150g of Licorice, add 16L of purified water, soak for 15min, boil for 30min, filter with 200-mesh filter cloth, add purified water to the residue and extract twice, add 8L of water each time, boil for 30min, combine the filtrate to get 20L, add 4ml of pectinase, 4ml of amylase, and 8ml of protease , keep warm at 50-55℃ for 60min, concentrate to 4L, cool to room temperature, add 6L of 80% ethanol solution, let stand for 24h, take the supernatant, add 100g of diatomaceous earth, add Buchner funnel covered with filter paper during stirring, vacuum filter, recover ethanol under reduced pressure, concentrate to 3000ml, and pack into tube glass bottles at 10ml / piece, cover, sterilize at 115℃ for 15min, cool, and obtain Dendrobium herbal tea extract. The recommended dosage for adults is 30ml per day.

[0036] Example 4 Preparation of herbal tea beverage

[0037] Take 2000 ml of the ethanol extract prepared in Example 2, recover the ethanol, evaporate it until there is no ethanol taste, add 448 g of xylitol, 22 g of citric acid, 2.2 g of sodium citrate, 1.7 g of sodium D-isoascorbate, and 0.90 g of sucralose, add purified water to dissolve completely and dilute to 11.2 L, pack it in tinplate cans at 280 ml / can, cover it, sterilize it at 115 ° C for 15 min, cool it, and obtain a dendrobium herbal tea beverage. The recommended dosage for adults is 2 times a day, 1 can each time.

[0038] Example 5 Preparation of concentrated herbal tea beverage

[0039] Take 2000 ml of the ethanol extract prepared in Example 2, recover the ethanol, evaporate it until there is no ethanol taste, add 36 g of maltitol, 0.6 g of β-cyclodextrin, and 0.12 g of sucralose, add purified water to dissolve completely and dilute to 1200 ml, and pack it into tube glass bottles at 20 ml / bottle, cover it, sterilize it at 115°C for 10 min, and cool it to obtain a concentrated dendrobium herbal tea beverage. The recommended dosage for adults is 1 bottle 3 times a day.

[0040] Example 6 Preparation of solid beverage

[0041] Take 4000 ml of the ethanol extract prepared in Example 2, recover the ethanol, concentrate it to a thick paste, dry it under vacuum at 80°C, and obtain 116.2 g of the extract. Grind it through an 80-mesh sieve, take 100 g of the extract powder, add 170 g of mannitol, 170 g of maltodextrin, and 0.5 g of sucralose, mix them in equal amounts, and pack them in PET / AL / PE composite film bags at 5 g / bag. Add 100-150 ml of warm boiled water to each bag and it can be drunk after being prepared. The recommended dosage for adults is 2-3 times a day, 1 bag each time.

[0042] Example 7 Preparation of herbal tea beverage

[0043] (1) Raw material extraction

[0044] Weigh 100kg of Dendrobium candidum, 75kg of Pueraria root, 75kg of Polygonatum, 50kg of Chrysanthemum, 50kg of Honeysuckle, 100kg of Poria, 100kg of Coix seeds, and 50kg of Licorice, put them into a 5T reflux extraction tank, add 3T of purified water, boil, reflux extract for 1h, 3 times in total, combine the extracts, concentrate under reduced pressure to 1.5T, control the temperature at 50-55℃, add 300ml of pectinase, 300ml of amylase, and 600ml of protease, keep warm for 60min, boil, cool to room temperature, add 1.5T of edible ethanol, let stand for 36h, take the supernatant, add 12kg of diatomaceous earth, filter on plate and frame, recover ethanol by reduced pressure in the filtrate, concentrate until there is no alcohol taste, add purified water to make the volume to 1500L, and stir evenly.

[0045] (2) Beverage production

[0046] Take 300kg of the extraction solution, pump it into a 10T blending tank, add 186kg of xylitol, 12.4kg of citric acid, 1.24kg of sodium citrate, 0.48kg of sucralose, and 0.31kg of caramel color, add purified water to make up to 6200L, stir evenly, boil, pump it into a temporary storage tank, and continuously blend 5 times for a total of 31T according to the production process and formula; fill it into prefabricated tinplate three-piece cans on a beverage filling machine at 310ml / can, press the cap, sterilize at 121℃ for 20min, and cool to obtain a dendrobium herbal tea beverage with a specification of 310ml / can and a batch size of 100,000 cans / batch. The recommended dosage for adults is 1 can 3 times a day.

[0047] (3) Product Inspection

[0048] According to GB / T 31326-2014 "Plant Beverages", batch 20220810 of products meets the standard requirements. The inspection results are shown in Table 2.

[0049] Table 2 Test results of dendrobium herbal tea beverage

[0050]

[0051] (4) Taste

[0052] The produced products were randomly distributed to 30 people aged 25 to 60 years old, including 18 males and 12 females. They drank the products within 2 to 4 hours after a meal and the taste was evaluated through a questionnaire survey. One young female participant responded that the Chinese medicine taste was too strong and unacceptable, while the other 29 people rated the taste as good or acceptable. The results are shown in Table 3.

[0053] Table 3 Statistical results of taste survey on dendrobium herbal tea beverages

[0054] Rating level Evaluation Criteria Number of people who rated 1 Sweet and sour, good taste 11 2 Moderate sweetness and sourness, average taste 12 3 Poor taste, but acceptable 6 4 The taste is very bad, unacceptable 1

[0055] Application Examples

[0056] 1. Effects on defecation function and xylene-induced ear swelling in normal mice

[0057] 1. Experimental Materials

[0058] Clean-grade ICR mice, weighing 18-22 g, were adaptively raised in the animal room environment for 5 days before the experiment, with an ambient temperature of 20-25°C and a relative humidity of 40-70%.

[0059] The test substance is the extract solution prepared in Example 3 of the present invention, and the dosage groups are 2.5, 5.0, and 10 ml / kg (equivalent to 5, 10, and 20 times the recommended human dosage of 30 ml / d). The sample is prepared with purified water to the concentration required for gavage, and is prepared and used immediately. The extract solution is stored at 2-8°C for no more than 1 day after opening.

[0060] The control drug was Coptis chinensis supernatant tablets, each weighing 0.31 grams. The recommended dosage for humans is 2 times a day, 6 tablets each time. Grind 5 tablets into powder, prepare 50 ml with purified water, and store at 2-8°C for no more than 3 days. The dosage for mice is 0.62g / kg (10 times the recommended dosage for humans).

[0061] The experiment set up 5 groups, including a control group, a drug control group and 3 test sample groups, with 10 mice in each group, half of them were male and half were female. They were randomly divided according to their body weight and given different doses of test samples by oral gavage with a gavage volume of 20 ml / kg, once a day for 10 consecutive days. The control group was given the same volume of purified water.

[0062] 2. Effects on the defecation function of normal mice

[0063] The mice were given drugs by oral gavage for 7 days continuously, and fasted for 16 hours but not water. When preparing the test solution, the ink suspension (weighing 20g of gum arabic, adding 160ml of water, boiling until the solution is transparent, adding 10g of powdered activated carbon, boiling, adding water to 200ml after the solution cools, storing at 2-8℃, and shaking before use) was added to the test sample in a ratio of 1:1. After oral gavage, each animal was raised individually, drinking water and eating normally, and the time of the first black stool, the number of black stool particles within 4 hours, and the defecation characteristics of each animal were observed and recorded. The results are shown in Table 4.

[0064] Table 4 Effect of Dendrobium herbal tea on defecation function of normal mice (X±SD, N=10)

[0065]

[0066] Compared with the model control group, *P<0.05.

[0067] The experimental results showed that compared with the control group, the number of bowel movements and the amount of unformed feces in the Huanglian Shangqing Tablets group of mice increased significantly, and there was no statistical difference between the test sample groups and the control group, indicating that the control drug Huanglian Shangqing Tablets had a certain laxative effect on normal mice, and the samples provided by the present invention would not affect the defecation function of normal mice.

[0068] 3. Effect of p-xylene on ear swelling in normal mice

[0069] The drug was administered by gavage continuously for 10 days. One hour after the last administration of the test substance, 20ul of xylene was evenly smeared on the outer auricle of the right ear of the mouse. After 30 minutes, the mouse was killed by cervical dislocation, and both ears were cut off. The ear pieces were punched in the same part of both ears with a 9mm puncher and weighed. The difference in weight between the two ears was the swelling degree of the auricle, and the swelling rate of the auricle was calculated with the left ear as the control. The results are shown in Table 5.

[0070] Table 5 Effect of Dendrobium herbal tea on ear swelling rate of mice (X±SD, n=10)

[0071]

[0072] Compared with the model control group, *P<0.05, **P<0.01.

[0073] Compared with the control group, the test sample Dendrobium herbal tea extract at doses of 5.0 and 10 ml / kg (10 and 20 times the recommended human dose) can significantly reduce the xylene-induced ear swelling of mice, with inhibition rates of 17.37% and 25.43% respectively, indicating that the test sample has a certain anti-inflammatory effect.

[0074] 2. Effects on Alloxan-induced Diabetes Model Mice

[0075] 1. Experimental Materials

[0076] Clean-grade ICR mice, male, weighing 18-22 g, were adaptively raised in the animal room environment for 5 days before the experiment, with an ambient temperature of 20-25°C and a relative humidity of 40-70%.

[0077] The test substance is the extract solution prepared in Example 3 of the present invention, and the dosage groups are 2.5, 5.0, and 10 ml / kg (equivalent to 5, 10, and 20 times the recommended dosage for humans), and the sample is prepared with purified water to the concentration required for oral administration. It is prepared and used immediately. The extract solution is stored at 2-8°C for no more than 1 day after opening.

[0078] The control drug was Shenqi Jiangtang Capsule, each capsule weighing 0.35 grams. The dosage was 3 times a day, 3 capsules each time. The contents of 3 capsules were taken and prepared into 40 ml with purified water. It was stored at 2-8°C for no more than 3 days. The dosage for mice was 0.525g / kg (10 times the recommended dosage for humans).

[0079] 2. Experimental methods

[0080] Healthy ICR male mice were adaptively fed for 5 days, fasted for 8 hours, and injected with alloxan 45 mg / kg through the tail vein with a dosing volume of 10 ml / kg. On the 7th day after the injection of alloxan, they were fasted for 4 hours, and the tail was cut off to collect blood sugar. Blood sugar was measured with a glucose determination kit. Diabetic model mice with blood sugar values ​​of 10-25 mmol / L were selected and randomly divided into 6 groups according to blood sugar values ​​and body weight, including blank control group, model control group, drug control group and 3 test sample groups, with 12 mice in each group. The corresponding test samples were given by gavage with a gavage volume of 20 ml / kg. The control group was given the same volume of purified water once a day for 4 consecutive weeks. The body weight was weighed once a week, and the dosing volume was adjusted according to the body weight.

[0081] From the 21st to the 26th day after administration, the 24-hour feed and water consumption of the whole cage animals was monitored for 5 consecutive days, the animal conditions were observed, and the average food intake and water intake were calculated. On the 28th day after administration, the animals were fasted for 4 hours, the tails were cut off and blood was collected, and the blood sugar level was measured using a glucose determination kit.

[0082] (1) Effects on the general condition of diabetic model mice

[0083] During the experiment, 2 animals died in the model control group and 1 died in the Dendrobium herbal tea extract 2.5 ml / kg group; by the 21st day, the body weight of the animals in the model control group was lower than that in the blank control group, and there was no statistical difference in the body weight of the animals in each drug-treated group compared with the control group, as shown in Table 6; through experimental observation, the animals in the model control group had dull fur color and were in poor condition, while the animals in the drug control Shenqi Jiangtang Capsule group and the Dendrobium herbal tea extract-treated groups had smooth fur color and were in good overall condition.

[0084] Table 6 Effect of Dendrobium herbal tea on body weight of diabetic mice (X±SD, n≥10)

[0085]

[0086]

[0087] (2) Effects on eating and drinking of diabetic mice

[0088] By measuring the 24-hour feed and diet consumption of the whole cage of animals, and calculating the average food intake and diet amount of diabetic mice for 5 days based on body weight, the average food intake and average diet amount of the model group animals were significantly higher than those of the blank control group, indicating that the model animals had developed typical diabetic symptoms of polydipsia and polyphagia; the average water intake of animals in the drug control Shenqi Jiangtang Capsule group and the Dendrobium Herbal Tea Extract 5.0ml / kg and 10ml / kg dose groups was significantly lower than that in the model control group, indicating that the test samples had a certain improvement effect on the diabetic symptoms of model mice; the average food intake of animals in each drug-treated group was lower than that of the model control group, but the difference was not statistically significant, see Table 7.

[0089] Table 7 Effects of Dendrobium herbal tea on food intake and diet of diabetic mice (X±SD, n=5)

[0090]

[0091] Compared with the normal group, # P<0.05, ## P<0.01; compared with the model group, *P<0.05.

[0092] (3) Effects on blood glucose in diabetic mice

[0093] Before administration, the blood glucose level of the model group animals was significantly higher than that of the blank control group, and there was no significant difference in the blood glucose level of mice in each administration group compared with the model group, indicating that the animal model was successfully replicated and the basal blood glucose levels of animals in each group were similar. After 28 days of administration, the blood glucose level of animals in the model group was slightly higher than that before administration, indicating that the mice still maintained the diabetic model state; compared with the model group, the blood glucose levels of animals in the drug control Shenqi Jiangtang Capsule group and the Dendrobium Herbal Tea Extract 10ml / kg group were significantly decreased, indicating that the test samples had a certain effect on reducing the blood glucose of diabetic model mice, as shown in Table 8.

[0094] Table 8 Effect of Dendrobium herbal tea on blood glucose in diabetic mice (X±SD, n≥10)

[0095]

[0096]

[0097] Compared with the normal group, ## P<0.01; compared with the model group, *P<0.05, **P<0.01.

[0098] 3. Acute toxicity test in mice

[0099] 1. Experimental Materials

[0100] SPF grade Kunming mice, weighing 18-22g, were adaptively raised for one week before the experiment. The breeding environment temperature was 20-26℃ (daily temperature difference ≤3℃), relative humidity was 40-70%, ventilation rate was 10-20 times / h, air flow velocity was 0.1-0.2m / s, noise was ≤60dB, working illumination was >300lx, animal illumination was 15-20lx, and light and dark alternation was used for 12h.

[0101] The test substance is the Dendrobium herbal tea extract powder prepared in Example 6 of the present invention, which is converted into a recommended human dosage of 2.91 g / d. The maximum dosage in the acute toxicity test in mice is 200 times the recommended human dosage. It is prepared into a 0.242 g / ml suspension with purified water and administered by gavage at 40 mL / kg.

[0102] 2. Experimental methods

[0103] The mice were adaptively fed for 1 week, fasted but not watered for 12 h, and randomly divided into 2 groups, 10 mice in each group, half male and half female. The test substance was administered once by gavage according to the maximum dosage method, and the control group was given an equal volume of purified water. The feed was fed 2 h after administration. The mice were observed for 4 hours after gavage of each sample, and then the animals' diet, appearance, behavioral activities, secretions, excretions, etc. were observed every day for abnormalities, as well as mortality, symptoms of poisoning reactions and their onset, severity, duration, reversibility, and recovery time, for a total of 14 days. After the observation period, blood was collected from the orbital vein and centrifuged at 4000 rpm for 10 min to separate serum. ALT, AST, BUN, and Cre were determined by colorimetry. The animals were killed and dissected to examine organ damage.

[0104] 3. Experimental results

[0105] During the acute toxicity test in mice, the animals' diet, appearance, behavioral activities, secretions, and excretions were normal, and no symptoms of poisoning reactions were shown. No deaths occurred by the end of the observation period. Therefore, the LD50 of the test substance was > 9.70 g / kg.

[0106] During the experimental observation period, the animals were weighed once a week. Compared with the control group, there was no significant difference in the weight gain of the mice. The weight weighing results at 0, 7, and 14 days are shown in Table 9.

[0107] Table 9 Observation of body weight of mice in acute toxicity test

[0108]

[0109] After the observation period, there was no significant difference in ALT, AST, BUN, Cre and other indicators of the mice compared with the control group, and no obvious organ damage was found in the anatomical examination. The results are shown in Table 10.

[0110] Table 10 Acute toxicity test of mouse liver and kidney function

[0111]

[0112] In the above test, the Dendrobium herbal tea extract prepared in Example 6 of the present invention was administered to normal mice by gavage at a single dose of 200 times the recommended human dosage, and no acute toxic reaction related to the test substance was found, indicating that the test sample Dendrobium herbal tea has a high safety.

[0113] 4. Experimental Conclusion

[0114] Animal Testing:

[0115] (1) Heat syndrome in traditional Chinese medicine includes symptoms such as red and swollen eyes, erosion at the corners of the mouth, yellow urine, toothache, sore throat, etc. The body often has different degrees of inflammatory response. Dendrobium herbal tea extract can reduce the swelling of the ear of mice caused by xylene, indicating that Dendrobium herbal tea has a certain anti-inflammatory effect and can relieve the symptoms of heat syndrome.

[0116] (2) Some drugs for relieving the symptoms of internal heat have a strong laxative effect, which often leads to gastrointestinal discomfort such as diarrhea, abdominal distension, and abdominal pain after taking them. By observing the frequency of defecation and fecal characteristics of mice, it was found that the Dendrobium herbal tea extract had no significant effect on the defecation function of normal mice, indicating that Dendrobium herbal tea can relieve the symptoms of internal heat without causing diarrhea.

[0117] (3) Diabetes is a thirst caused by insufficient body fluid and internal heat due to yin deficiency, which is manifested by polydipsia, polyphagia, polyuria, and weight loss. Dendrobium herbal tea extract can reduce the average water intake and fasting blood sugar of diabetic model mice, and also has a certain effect on the average food intake of mice, indicating that Dendrobium herbal tea has an improving effect on diabetic symptoms.

[0118] (4) In the acute toxicity test on mice, no obvious toxic reaction was observed when the Dendrobium herbal tea extract was administered at 200 times the recommended human dosage, and the LD50 was greater than 9.70 g / kg, indicating a high safety level.

[0119] In summary, the dendrobium herbal tea provided by the present invention can help people with yin deficiency and hyperactivity of fire to improve conditions such as yin damage and fluid deficiency, dry mouth and thirst, and post-illness heat, without causing diarrhea, abdominal pain and other uncomfortable symptoms caused by decreased spleen and stomach function. It is especially suitable for insufficient body fluid and yin deficiency and internal heat caused by diabetes.

[0120] Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made in form and details without departing from the scope of the present invention as defined in the claims.

Claims

1. A dendrobium herbal tea, characterized in that: The weight proportions of the components of the dendrobium herbal tea are: 3-4 parts of dendrobium officinale, 2-3 parts of kudzu root, 2-3 parts of polygonatum, 1-2 parts of chrysanthemum, 1-2 parts of honeysuckle, 3-4 parts of tuckahoe, 3-4 parts of coix seeds, and 1-2 parts of liquorice.

2. The method for preparing a dendrobium herbal tea according to claim 1, characterized in that The preparation process includes the following steps: (1) Prepare materials according to the formula, and all raw materials are commercially available decoction pieces; (2) taking each component in the formula, extracting it 2-3 times with purified water 15-20 times the weight of the medicinal material, each time for 30-60 minutes, combining the extracts, filtering, adding 0.01-0.02% pectinase, 0.01-0.02% amylase, and 0.02-0.04% protease according to the amount of the filtrate, keeping it at 50-55° C. for 30-60 minutes, concentrating it to a volume 2-3 times the weight of the medicinal material, and cooling it to room temperature; (3) adding 1 to 2 times the volume of the concentrate by mass, 80% or more ethanol solution, so that the ethanol concentration in the solution reaches 40% or more, leaving it to stand for more than 12 hours, taking the supernatant, adding diatomaceous earth for fine filtration, recovering ethanol, and concentrating it to a volume of 2 to 3 times the mass of the medicinal material; (4) Add auxiliary materials, adjust to the required volume, mix, fill, and sterilize to obtain the finished product.

3. The use of a dendrobium herbal tea according to claim 2, characterized in that: Dendrobium herbal tea can be used to improve the symptoms of diabetes. The adult dosage is 2 to 3 times a day, 1 bottle each time, which is equivalent to consuming 16 to 24 grams of the original medicinal material per day.

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