Moisturizing and tightening composition containing type III collagen

Abstract

The invention relates to a moisturizing and tightening composition containing type III collagen, and belongs to the technical field of cosmetics. In the composition disclosed by the invention, the III-type collagen and the Chinese ilex leaf water extract have a synergistic effect, so that the moisture content in the skin is remarkably increased, and barrier repair is promoted, and therefore, the moisturizing and water locking capacities of the skin are enhanced. The oat extract can lock skin moisture, promote skin metabolism and improve skin roughness at the same time, and when the oat extract and the III-type collagen are used together, the compactness and smoothness of the skin can be further improved; the centella asiatica alcohol extract can promote synthesis of collagen and accelerate healing of skin wounds, and when the centella asiatica alcohol extract is combined with type III collagen, the moisturizing and tightening effects of the skin can be remarkably enhanced; when the chrysanthemum indicum alcohol extract and the III-type collagen are used together, the instant and long-acting anti-wrinkle effect on human skin is remarkable, and the skin tightening effect is enhanced.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a moisturizing and firming composition containing type III collagen. Background Art

[0002] Type III collagen is an important structural protein that is widely present in elastic tissues such as human skin, tendons and blood vessels. It plays a vital role in maintaining skin elasticity, promoting wound healing and maintaining the integrity of blood vessels.

[0003] With the development of biotechnology, in the field of skin care, based on its superior biocompatibility and ability to promote skin regeneration, type III collagen has the potential to be added as an effective ingredient to moisturizing and firming skin care products. Summary of the invention

[0004] The object of the present invention is to provide a moisturizing and firming composition containing type III collagen, wherein the type III collagen component is added as an effective component to a moisturizing and firming skin care product.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] A moisturizing and firming composition containing type III collagen, comprising the following raw materials in parts by weight:

[0007] Type III collagen 5-20 parts

[0008] 8-10 parts camellia oil

[0009] Moisturizer 10-30 parts

[0010] 1-5 parts of antioxidant

[0011] 6-20 parts of plant extract

[0012] The plant extract consists of holly leaf water extract, oat extract, centella asiatica alcohol extract, scutellaria baicalensis root alcohol extract and spiltnia chrysanthemum alcohol extract.

[0013] As a preferred embodiment of the present invention, in the plant extracts, the mass ratio of ilex leaf water extract, oat extract, Centella asiatica alcohol extract, Scutellaria baicalensis root alcohol extract and Spinach chamomile alcohol extract is 2-5:1-3:1-4:1-2:1-3.

[0014] As a preferred embodiment of the present invention, the holly leaf water extract is prepared by the following operation: mixing holly leaves with water at 70-80° C. and extracting for 4-6 hours.

[0015] As a preferred embodiment of the present invention, the alcohol extract of Centella asiatica is prepared by the following operation: extracting Centella asiatica with 60-70 wt% ethanol solution at 65-75° C. for 2-4 hours.

[0016] As a preferred embodiment of the present invention, the ethanol extract of the root of Scutellaria baicalensis is prepared by the following operation: extracting the root of Scutellaria baicalensis with 70-80wt% ethanol solution for 1.5-2h.

[0017] As a preferred solution of the present invention, the alcohol extract of spiltnia is prepared by the following operation: extracting spiltnia with 80-95wt% ethanol solution at 50-60°C for 1-3h.

[0018] As a preferred embodiment of the present invention, the moisturizing agent is at least one of glycerin, sodium hyaluronate, and butylene glycol, and / or the antioxidant is at least one of vitamin E and vitamin C.

[0019] As a preferred embodiment of the present invention, the raw material further comprises urea.

[0020] As a preferred embodiment of the present invention, the composition is prepared by the following operations:

[0021] Mix the moisturizer, antioxidant, holly leaf water extract, oat extract and distilled water, heat to 70-80° C., and mix well to prepare phase A;

[0022] Mix camellia seed oil, type III collagen, Centella asiatica alcohol extract, Scutellaria baicalensis root alcohol extract and prepared Spinach chamomile alcohol extract, heat to 70-80° C., mix evenly, and prepare phase B;

[0023] Phase A, phase B and an emulsifier are uniformly mixed to obtain an emulsion, and the pH value is adjusted to obtain a moisturizing and firming composition containing type III collagen.

[0024] As a preferred embodiment of the present invention, the pH adjustment is to adjust the pH value of the mixture to 5.5-6.5 using an acid-base regulator, and the acid-base regulator is at least one of citric acid and sodium hydroxide.

[0025] Beneficial effects of the present invention:

[0026] The moisturizing and firming composition containing type III collagen of the present invention uses type III collagen, camellia seed oil and plant extracts, and the plant extracts are holly leaf water extract, oat extract, Centella asiatica alcohol extract, scutellaria root alcohol extract, and schizonepeta alcohol extract. Among them, type III collagen and holly leaf water extract have a synergistic effect, significantly increasing the moisture content in the skin and promoting barrier repair, thereby enhancing the skin's moisturizing and water-locking ability. Oat extract can lock in skin moisture, while promoting skin metabolism and improving skin roughness. When used together with type III collagen, it can further improve the firmness and smoothness of the skin; Centella asiatica alcohol extract can promote the synthesis of collagen and accelerate skin wound healing. When combined with type III collagen, it can significantly enhance the moisturizing and firming effects of the skin; when schizonepeta alcohol extract is used together with type III collagen, it has significant immediate and long-lasting anti-wrinkle effects on human skin and enhances the firming effect of the skin;

[0027] Furthermore, when urea is added to the raw materials of the composition of the present invention, the moisturizing effect is significantly enhanced, and the anti-wrinkle effect of the composition is also synergistically enhanced, which shows that urea and the components of the composition have a synergistic effect. DETAILED DESCRIPTION

[0028] The present invention is further described in detail below in conjunction with specific implementation methods.

[0029] The camellia seed oils described below were all purchased from Guangzhou Artemisia Biotechnology Co., Ltd., CAS: 68916-73-4; the oat extracts were all purchased from Shanxi Yerui Biotechnology Co., Ltd., CAS / molecular formula: 160872-27-5, which will not be repeated here.

[0030] Example 1

[0031] Preparation of a moisturizing and firming composition containing type III collagen

[0032] (1) Preparation of holly leaf aqueous extract: Grind the holly leaves and put them into an extraction tank, add distilled water and stir evenly, transfer to a constant temperature water bath and magnetically stir at 70°C for 4 hours, cool to room temperature after stirring, filter, vacuum filter, and dry to obtain the holly leaf extract.

[0033] (2) Preparation of Centella asiatica alcohol extract: Crush the Centella asiatica raw material into powder, extract it with 60wt% ethanol solution at 65°C for 2h, with a solid-liquid ratio of 1:10, extract it twice, combine the extracts, concentrate under reduced pressure to remove the solvent, and obtain the Centella asiatica extract after drying.

[0034] (3) Preparation of ethanol extract of Scutellaria root: reflux the solution three times with 70 wt% ethanol solution of 6 times the mass of Scutellaria root raw material, each time for 1.5 h, collect and combine the filtrates, concentrate under reduced pressure to 0.5 times the mass of the raw material, extract with an equal volume of petroleum ether three times, extract with n-butanol, collect the alcohol layer, and concentrate under reduced pressure to obtain the Scutellaria root extract.

[0035] (4) Preparation of Globe Angelica dahurica alcohol extract: fresh, dry, impurity-free Globe Angelica dahurica flowers are selected as raw materials; the Globe Angelica dahurica flowers are crushed into 80 meshes by a grinder; 80 wt % ethanol solution is used for extraction at 50° C. for 1 h, with a solid-liquid ratio of 1:15, and the extraction is performed twice. The extracts are combined, concentrated under reduced pressure to remove the solvent, and dried to obtain the Globe Angelica dahurica extract.

[0036] (5) Preparation of a moisturizing and firming composition containing type III collagen:

[0037] Mix glycerin, a moisturizer, vitamin E, an antioxidant, an aqueous extract of holly leaves, and an oat extract, add distilled water, heat to 70° C., and mix well to prepare phase A;

[0038] Mix camellia seed oil, type III collagen, Centella asiatica alcohol extract, Scutellaria baicalensis root alcohol extract and prepared Spinach chamomile alcohol extract, heat to 70° C., mix well, and prepare phase B;

[0039] Slowly add phase A to phase B while stirring, continue stirring until all ingredients are fully blended, then add emulsifier sucrose stearate for emulsification to obtain an emulsion, use acid-base regulators (citric acid, sodium hydroxide) to adjust the pH value of the mixture to 5.5-6.5, stir evenly, and obtain a moisturizing and firming composition containing type III collagen.

[0040] Example 2

[0041] Preparation of a moisturizing and firming composition containing type III collagen

[0042] (1) Preparation of holly leaf aqueous extract: crush the holly leaves and put them into an extraction tank, add distilled water and stir evenly, transfer to a constant temperature water bath and magnetically stir at 72°C for 4.5 hours, cool to room temperature after stirring, filter, vacuum filter, and dry to obtain the holly leaf extract.

[0043] (2) Preparation of Centella asiatica alcohol extract: Crush the Centella asiatica raw material into powder, extract it with 62 wt% ethanol solution at 68°C for 2.5 h, with a solid-liquid ratio of 1:12, extract it twice, combine the extracts, concentrate under reduced pressure to remove the solvent, and obtain the Centella asiatica extract after drying.

[0044] (3) Preparation of ethanol extract of Scutellaria root: reflux 72 wt % ethanol solution with 7 times the mass of Scutellaria root raw material three times, each time for 1.5 h, collect and combine the filtrates, concentrate under reduced pressure to 0.8 times the mass of the raw material, extract with an equal volume of petroleum ether three times, extract with n-butanol, collect the alcohol layer, and concentrate under reduced pressure to obtain the Scutellaria root extract.

[0045] (4) Preparation of Globe Angelica dahurica ethanol extract: fresh, dry, impurity-free Globe Angelica dahurica flowers were selected as raw materials; the Globe Angelica dahurica flowers were crushed into 80 meshes by a grinder; 83 wt % ethanol solution was used for extraction at 52° C. for 1.5 h, with a solid-liquid ratio of 1:16, and the extraction was performed twice. The extracts were combined, concentrated under reduced pressure to remove the solvent, and dried to obtain Globe Angelica dahurica extract.

[0046] (5) Preparation of a moisturizing and firming composition containing type III collagen:

[0047] Mix butylene glycol, an antioxidant, vitamin C, an aqueous extract of holly leaves, and an oat extract, add distilled water, heat to 72° C., and mix well to prepare phase A;

[0048] Mix camellia seed oil, type III collagen, Centella asiatica alcohol extract, Scutellaria baicalensis root alcohol extract and prepared Spinach chamomile alcohol extract, heat to 72° C., mix well, and prepare phase B;

[0049] Slowly add phase A to phase B while stirring, continue stirring until all ingredients are fully blended, then add emulsifier sucrose stearate for emulsification to obtain an emulsion, use acid-base regulators (citric acid, sodium hydroxide) to adjust the pH value of the mixture to 5.5-6.5, stir evenly, and obtain a moisturizing and firming composition containing type III collagen.

[0050] Example 3

[0051] Preparation of a moisturizing and firming composition containing type III collagen

[0052] (1) Preparation of holly leaf aqueous extract: crush the holly leaves and put them into an extraction tank, add distilled water and stir evenly, transfer to a constant temperature water bath and stir magnetically at 75°C for 5 hours, cool to room temperature after stirring, filter, vacuum filter, and dry to obtain the holly leaf extract.

[0053] (2) Preparation of Centella asiatica alcohol extract: Crush the Centella asiatica raw material into powder, extract it with 65wt% ethanol solution at 70°C for 3h, with a solid-liquid ratio of 1:15, extract it 3 times, combine the extracts, concentrate under reduced pressure to remove the solvent, and obtain the Centella asiatica extract after drying.

[0054] (3) Preparation of ethanol extract of Scutellaria root: reflux 75 wt % ethanol solution with 8 times the mass of Scutellaria root raw material three times, each time for 1.6 h, collect and combine the filtrates, concentrate under reduced pressure to 1 times the mass of the raw material, extract with an equal volume of petroleum ether three times, extract with n-butanol, collect the alcohol layer, and concentrate under reduced pressure to obtain the Scutellaria root extract.

[0055] (4) Preparation of Globe Angelica dahurica ethanol extract: fresh, dry, impurity-free Globe Angelica dahurica flowers were selected as raw materials; the Globe Angelica dahurica flowers were crushed into 100 meshes by a grinder; 88 wt % ethanol solution was used for extraction at 55° C. for 2 h, with a solid-liquid ratio of 1:18, and the extraction was performed twice. The extracts were combined, concentrated under reduced pressure to remove the solvent, and dried to obtain Globe Angelica dahurica extract.

[0056] (5) Preparation of a moisturizing and firming composition containing type III collagen:

[0057] Mix the moisturizing agent sodium hyaluronate, the antioxidant vitamin E, the holly leaf water extract and the oat extract, add distilled water, heat to 75° C., mix well, and prepare phase A;

[0058] Mix camellia seed oil, type III collagen, Centella asiatica alcohol extract, Scutellaria baicalensis root alcohol extract and prepared Spinach chamomile alcohol extract, heat to 75° C., mix well, and prepare phase B;

[0059] Slowly add phase A to phase B while stirring, continue stirring until all ingredients are fully blended, then add emulsifier sucrose stearate for emulsification to obtain an emulsion, use acid-base regulators (citric acid, sodium hydroxide) to adjust the pH value of the mixture to 5.5-6.5, stir evenly, and obtain a moisturizing and firming composition containing type III collagen.

[0060] Example 4

[0061] Preparation of a moisturizing and firming composition containing type III collagen

[0062] (1) Preparation of holly leaf aqueous extract: crush the holly leaves and put them into an extraction tank, add distilled water and stir evenly, transfer to a constant temperature water bath and magnetically stir at 78°C for 5.5 hours, cool to room temperature after stirring, filter, vacuum filter, and dry to obtain the holly leaf extract.

[0063] (2) Preparation of Centella asiatica alcohol extract: Crush the Centella asiatica raw material into powder, extract it with 68wt% ethanol solution at 72°C for 3.5h, with a solid-liquid ratio of 1:18, extract it three times, combine the extracts, concentrate under reduced pressure to remove the solvent, and obtain the Centella asiatica extract after drying.

[0064] (3) Preparation of ethanol extract of Scutellaria root: reflux 3 times with 78 wt % ethanol solution of 9 times the mass of Scutellaria root raw material, each time for 2 h, collect and combine the filtrates, concentrate under reduced pressure to 1.2 times the mass of the raw material, extract 3 times with an equal volume of petroleum ether, extract with n-butanol, collect the alcohol layer, and concentrate under reduced pressure to obtain the Scutellaria root extract.

[0065] (4) Preparation of Globe Angelica dahurica alcohol extract: fresh, dry, impurity-free Globe Angelica dahurica flowers are selected as raw materials; the Globe Angelica dahurica flowers are crushed into 100 meshes by a grinder; 90 wt % ethanol solution is used for extraction at 58° C. for 2.5 h, with a solid-liquid ratio of 1:19, and the extraction is performed twice. The extracts are combined, concentrated under reduced pressure to remove the solvent, and dried to obtain the Globe Angelica dahurica extract.

[0066] (5) Preparation of a moisturizing and firming composition containing type III collagen:

[0067] Mix glycerin, a moisturizer, vitamin C, an antioxidant, holly leaf water extract, and oat extract, add distilled water, heat to 78° C., and mix well to prepare phase A;

[0068] Mix camellia seed oil, type III collagen, Centella asiatica alcohol extract, Scutellaria baicalensis root alcohol extract and prepared Spinach chamomile alcohol extract, heat to 78° C., mix well, and prepare phase B;

[0069] Slowly add phase A to phase B while stirring, continue stirring until all ingredients are fully blended, then add emulsifier sucrose stearate for emulsification to obtain an emulsion, use acid-base regulators (citric acid, sodium hydroxide) to adjust the pH value of the mixture to 5.5-6.5, stir evenly, and obtain a moisturizing and firming composition containing type III collagen.

[0070] Example 5

[0071] Preparation of a moisturizing and firming composition containing type III collagen

[0072] (1) Preparation of holly leaf aqueous extract: crush the holly leaves and put them into an extraction tank, add distilled water and stir evenly, transfer to a constant temperature water bath and magnetically stir at 80°C for 6 hours, cool to room temperature after stirring, filter, vacuum filter, and dry to obtain the holly leaf extract.

[0073] (2) Preparation of Centella asiatica alcohol extract: Crush the Centella asiatica raw material into powder, extract it with 70wt% ethanol solution at 75°C for 4h, with a solid-liquid ratio of 1:20, extract it 3 times, combine the extracts, concentrate under reduced pressure to remove the solvent, and obtain the Centella asiatica extract after drying.

[0074] (3) Preparation of ethanol extract of Scutellaria root: reflux 3 times with 80 wt% ethanol solution of 10 times the mass of Scutellaria root raw material, each time for 2 h, collect and combine the filtrates, concentrate under reduced pressure to 1.5 times the mass of the raw material, extract 3 times with an equal volume of petroleum ether, extract with n-butanol, collect the alcohol layer, and concentrate under reduced pressure to obtain the Scutellaria root extract.

[0075] (4) Preparation of Globe Angelica dahurica alcohol extract: fresh, dry, impurity-free Globe Angelica dahurica flowers are selected as raw materials; the Globe Angelica dahurica flowers are crushed into 100 meshes by a grinder; 95 wt % ethanol solution is used for extraction at 60° C. for 3 h, with a solid-liquid ratio of 1:20, and the extraction is performed twice. The extracts are combined, concentrated under reduced pressure to remove the solvent, and dried to obtain Globe Angelica dahurica extract.

[0076] (5) Preparation of a moisturizing and firming composition containing type III collagen:

[0077] Mix the moisturizing agent sodium hyaluronate, the antioxidant vitamin C, the holly leaf water extract and the oat extract, add distilled water, heat to 80° C., mix well, and prepare phase A;

[0078] Mix camellia seed oil, type III collagen, Centella asiatica alcohol extract, Scutellaria baicalensis root alcohol extract and prepared Spinach chamomile alcohol extract, heat to 80° C., mix well, and prepare phase B;

[0079] Slowly add phase A to phase B while stirring, continue stirring until all ingredients are fully blended, then add emulsifier sucrose stearate for emulsification to obtain an emulsion, use acid-base regulators (citric acid, sodium hydroxide) to adjust the pH value of the mixture to 5.5-6.5, stir evenly, and obtain a moisturizing and firming composition containing type III collagen.

[0080] Example 6

[0081] Preparation of a moisturizing and firming composition containing type III collagen

[0082] (1) Preparation of holly leaf aqueous extract: crush the holly leaves and put them into an extraction tank, add distilled water and stir evenly, transfer to a constant temperature water bath and magnetically stir at 80°C for 6 hours, cool to room temperature after stirring, filter, vacuum filter, and dry to obtain the holly leaf extract.

[0083] (2) Preparation of Centella asiatica alcohol extract: Crush the Centella asiatica raw material into powder, extract it with 70wt% ethanol solution at 75°C for 4h, with a solid-liquid ratio of 1:20, extract it 3 times, combine the extracts, concentrate under reduced pressure to remove the solvent, and obtain the Centella asiatica extract after drying.

[0084] (3) Preparation of ethanol extract of Scutellaria root: reflux 3 times with 80 wt% ethanol solution of 10 times the mass of Scutellaria root raw material, each time for 2 h, collect and combine the filtrates, concentrate under reduced pressure to 1.5 times the mass of the raw material, extract 3 times with an equal volume of petroleum ether, extract with n-butanol, collect the alcohol layer, and concentrate under reduced pressure to obtain the Scutellaria root extract.

[0085] (4) Preparation of Globe Angelica dahurica alcohol extract: fresh, dry, impurity-free Globe Angelica dahurica flowers are selected as raw materials; the Globe Angelica dahurica flowers are crushed into 100 meshes by a grinder; 95 wt % ethanol solution is used for extraction at 60° C. for 3 h, with a solid-liquid ratio of 1:20, and the extraction is performed twice. The extracts are combined, concentrated under reduced pressure to remove the solvent, and dried to obtain Globe Angelica dahurica extract.

[0086] (5) Preparation of a moisturizing and firming composition containing type III collagen:

[0087] Mix the moisturizing agent sodium hyaluronate, the antioxidant vitamin E, the holly leaf water extract, the oat extract and urea, add distilled water, heat to 80° C., mix well, and prepare phase A;

[0088] Mix camellia seed oil, type III collagen, Centella asiatica alcohol extract, Scutellaria baicalensis root alcohol extract and prepared Spinach chamomile alcohol extract, heat to 80° C., mix well, and prepare phase B;

[0089] Slowly add phase A to phase B while stirring, continue stirring until all ingredients are fully blended, then add emulsifier sucrose stearate for emulsification to obtain an emulsion, use acid-base regulators (citric acid, sodium hydroxide) to adjust the pH value of the mixture to 5.5-6.5, stir evenly, and obtain a moisturizing and firming composition containing type III collagen.

[0090] The weight parts of each component in Example 1 and Example 5 are shown in Table 1:

[0091] Table 1

[0092]

[0093]

[0094] Comparative Examples 1 to 4 Preparation of Moisturizing and Firming Compositions Containing Type III Collagen

[0095] The weight parts of the components in Comparative Examples 1 to 4 are as described in Table 2 below. Except for the weight parts of the components being different from those in Example 3, the other parts are the same as those in Example 3.

[0096] Table 2

[0097]

[0098]

[0099] The moisturizing and firming compositions containing type III collagen prepared in Examples 1 to 6 and Comparative Examples 1 to 4 were tested as follows:

[0100] The moisturizing and firming compositions containing type III collagen prepared in Examples 1 to 6 and Comparative Examples 1 to 4 were made into essences, the ingredients of which are shown in Table 3:

[0101] Table 3

[0102] Element Mass fraction (%) Compositions prepared from Examples 1 to 6 and Comparative Examples 1 to 4 1 Moisturizer 0.5 preservative 0.1 Polyols 8 Thickener 0.1 pH Adjusters Moderate water Add to 100

[0103] Test Example 1 Moisturizing Performance Test

[0104] 1. Experimental instruments

[0105] Multifunctional skin tester (including Cormeometer CM825 moisture test probe and Tewameter TM300 moisture loss test probe), German CK company.

[0106] 2. Test object and environment

[0107] Test subjects: A total of 30 subjects aged between 18 and 45 years old with healthy skin and no history of cosmetic allergies were screened. The volunteers read and signed the informed consent form before the test.

[0108] Test environment: temperature (21±1)℃, relative humidity (50%±10%).

[0109] 3. Experimental methods

[0110] The volunteers were randomly divided into 10 groups, 3 people in each group, and the essences prepared in Examples 1 to 6 and Comparative Examples 1 to 4 were used on the whole face. The essences were used every morning and evening for 4 weeks. The moisture (MMV) values ​​of the same areas of the left and right halves of the face of the subjects were measured before use (D0), after one week of use (D7), and after four weeks of continuous use (D28). The same area was measured 3 times and the average value was recorded. The results are shown in Table 4.

[0111] Table 4

[0112] Group Moisture (MMV) value (D0) Moisture (MMV) value (D7) Moisture (MMV) value (D28) Example 1 52.6 68.2 74.5 Example 2 50.4 66.9 73.2 Example 3 54.9 69.8 75.3 Example 4 49.8 65.5 72.4 Example 5 53.3 68.8 73.9 Example 6 56.8 70.7 80.3 Comparative Example 1 50.2 62.8 68.4 Comparative Example 2 54.1 58.3 62.9 Comparative Example 3 53.5 60.3 66.7 Comparative Example 4 56.8 61.0 69.8

[0113] As shown in Table 4, the moisture value of the essence prepared from the composition of Examples 1 to 5 was significantly increased after 7 days of use, all of which were greater than 65; after 28 days of use, the moisture value was greater than 70, which shows that the composition of the present invention can effectively increase the moisture content of the skin and has good moisturizing performance; Example 6 adds urea to the composition, and its moisturizing performance is better than that of Examples 1 to 5. In Comparative Examples 1 to 5, the moisture value of Comparative Example 2 on the 7th and 28th days is significantly reduced compared with that of Example 3, which shows that the holly leaf water extract has a synergistic effect with other ingredients in the composition; the moisture values ​​of Comparative Examples 1 and Comparative Examples 3 to 4 on the 7th and 28th days are reduced to varying degrees compared with Example 3.

[0114] Test Example 2 Test on the effect of fibroblast secretion of type I collagen

[0115] Test method: After the cells were inoculated and incubated overnight, they were washed with PBS and then grouped according to the test (see Table 5). The UVA irradiated group was irradiated with 30 J / cm 2 UVA irradiation. According to the cell viability test results obtained by the MTT test, the sample solution to be tested was prepared, TGF-β1 (100ng / mL) was used as the positive control, 100μL was added to each well, and 3 replicates were set for each group. After the administration was completed, the cells were incubated in the dark for 24h, and the cell supernatants corresponding to each sample group were collected into centrifuge tubes. After centrifugation, the supernatants were collected and placed in 1.5mL centrifuge tubes. The type I collagen content was tested according to the instructions of the ELISA kit. The results are shown in Table 6.

[0116] Table 5

[0117]

[0118] Table 6

[0119] Group Type I collagen content (ng / mL) Blank group 84.6 Positive control group 99.3 Example 1 95.8 Example 2 94.2 Example 3 96.7 Example 4 95.6 Example 5 95.3 Example 6 97.9 Comparative Example 1 91.4 Comparative Example 2 88.5 Comparative Example 3 90.7 Comparative Example 4 90.1

[0120] As shown in Table 6, the content of type I collagen increased significantly after using the compositions of Examples 1 to 5, all of which were greater than 94, indicating that the composition of the present invention has the ability to promote the synthesis of type I collagen; in Example 6, urea was added to the composition, and its type I collagen content increased more than that of Examples 1 to 5. Among Comparative Examples 1 to 5, the type I collagen content of Comparative Example 2 decreased significantly compared with that of Example 3, indicating that the holly leaf water extract and other components in the composition had a synergistic effect; the type I collagen content of Comparative Examples 1 and Comparative Examples 3 to 4 decreased to varying degrees compared with that of Example 3.

[0121] Test Example 3 Zebrafish firming and anti-wrinkle test

[0122] Wild AB zebrafish fry at 2 dpf (days post fertilization) were used for the test. According to the experimental grouping (see Table 7), 8 fry were divided into each group. The appropriate sample concentration was selected as the test concentration and 3 mL of the sample solution to be tested was added to each well. After the administration, the 6-well plate was placed in a 28°C incubator and incubated in the dark for 2 hours. The sample group was irradiated 3 times under ultraviolet light, each irradiation time was 15 minutes, the interval was 30 minutes, and the irradiation amount each time was (2.07+0.18) J / cm 2 The total radiation dose is (6.21±0.54) J / cm 2 , and then incubate in a light-proof incubator for 22 h.

[0123] After 22 hours, the 6-well plate was taken out, anesthetized with tricaine, and the fish were placed on a glass slide loaded with 2% methylcellulose. Then, a stereo microscope was used to take photos of the tail fin behind the cloaca of the juvenile fish. The tail fin area of ​​each fish was measured using the software ImageJ. The inhibition rate of zebrafish tail fin shrinkage was calculated according to the following formula. The results are shown in Table 8:

[0124]

[0125] Among them, Q is the inhibition rate of zebrafish tail fin wrinkling compared with the sample group and the model control group, and A1, A2, and A3 are the average values ​​of the zebrafish tail fin areas of the sample group, model control group, and blank control group, respectively.

[0126] Table 7

[0127]

[0128]

[0129] Table 8

[0130] Group Zebrafish tail fin wrinkling inhibition rate (%) Blank group (normal feeding) 100 Example 1 16.3 Example 2 16.5 Example 3 17.9 Example 4 17.2 Example 5 16.6 Example 6 18.1 Comparative Example 1 13.5 Comparative Example 2 11.7 Comparative Example 3 13.9 Comparative Example 4 13.8

[0131] As shown in Table 6, the inhibition rate of zebrafish tail fin wrinkling increased significantly after using the compositions of Examples 1 to 5, all of which were greater than 16, indicating that the compositions of the present invention have firming and anti-wrinkle effects; Example 6 added urea to the composition, and its inhibition rate of zebrafish tail fin wrinkling was higher than that of Examples 1 to 5. Among Comparative Examples 1 to 5, the inhibition rate of zebrafish tail fin wrinkling in Comparative Example 2 was significantly lower than that in Example 3, indicating that the holly leaf water extract and other components in the composition had a synergistic effect; the inhibition rates of zebrafish tail fin wrinkling in Comparative Examples 1 and Comparative Examples 3 to 4 were lowered to varying degrees compared with Example 3.

[0132] The above is only an implementation method of the invention, and does not limit the scope of patent protection. Those skilled in the art can make non-substantial changes or substitutions based on the invention, which still fall within the scope of patent protection.

Claims

1. A moisturizing and firming composition containing type III collagen, characterized in that: Contains the following raw materials in parts by weight: Type III collagen 5~20 parts Camellia oil 8-10 parts Moisturizer 10~30 parts 1~5 parts of antioxidant Plant extract 6~20 parts The plant extract consists of holly leaf water extract, oat extract, centella asiatica alcohol extract, scutellaria baicalensis root alcohol extract and spiltnia chrysanthemum alcohol extract.

2. The composition according to claim 1, characterized in that In the plant extracts, the mass ratio of ilex leaf water extract, oat extract, Centella asiatica alcohol extract, scutellaria root alcohol extract and spilanthus chrysantha alcohol extract is 2-5:1-3:1-4:1-2:1-3.

3. The composition according to claim 1, characterized in that The holly leaf water extract is prepared by the following operation: mixing the holly leaf with water and extracting at 70-80° C. for 4-6 hours.

4. The composition according to claim 1, characterized in that The Centella asiatica alcohol extract is prepared by the following operation: extracting Centella asiatica with 60-70wt% ethanol solution at 65-75°C for 2-4h.

5. The composition according to claim 1, characterized in that The ethanol extract of the root of scutellaria baicalensis is prepared by the following operation: extracting the root of scutellaria baicalensis with 70-80wt% ethanol solution for 1.5-2h.

6. The composition according to claim 1, characterized in that The spiltnia ethanol extract is prepared by the following operation: extracting spiltnia odorata with 80-95 wt % ethanol solution at 50-60° C. for 1-3 hours.

7. The composition according to claim 1, characterized in that The moisturizer is at least one of glycerin, sodium hyaluronate, and butylene glycol, and / or the antioxidant is at least one of vitamin E and vitamin C.

8. The composition according to claim 1, characterized in that The raw materials also contain urea.

9. The composition according to claim 1, characterized in that The composition is prepared by the following operations: Mix the moisturizer, antioxidant, holly leaf water extract, oat extract and distilled water, heat to 70-80°C, and mix well to prepare phase A; Mix camellia seed oil, type III collagen, Centella asiatica alcohol extract, Scutellaria baicalensis root alcohol extract and prepared Spinach chamomile alcohol extract, heat to 70-80° C., mix well, and prepare phase B; Phase A, phase B and an emulsifier are uniformly mixed to obtain an emulsion, and the pH value is adjusted to obtain a moisturizing and firming composition containing type III collagen.

10. The composition according to claim 9, characterized in that The pH adjustment is to adjust the pH value of the mixture to 5.5-6.5 using an acid-base regulator, and the acid-base regulator is at least one of citric acid and sodium hydroxide.

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