Gradient geochemical environment microorganism enrichment and separation system

Abstract

The invention discloses a gradient geochemical environment microorganism enrichment and separation system, which comprises: a glass barrel, the side wall of the glass barrel is vertically provided with a plurality of sampling ports, the lower end of the side wall of the glass barrel is provided with a circulation inlet, the upper end of the side wall of the glass barrel is provided with a circulation outlet, and the upper end of the glass barrel is provided with a material supplementing port; the detection module is used for detecting geochemical and nutrient element characteristics of the extracted microorganism sample; and the design module is used for designing a corresponding solid or liquid culture medium according to the detected geochemical and nutrient element characteristics and further carrying out separation and purification. According to the invention, the enrichment and separation efficiency of microorganisms is improved.

Description

Technical Field

[0001] The invention relates to a gradient geochemical environment microorganism enrichment and separation system. Background Art

[0002] In recent years, microbial isolation and culture technology has occupied an important position in microbiological research. By improving the culture medium formula and culture conditions, researchers can cultivate more types of microorganisms. In addition, modern molecular biology techniques such as 16S rRNA gene sequencing have also been widely used in the identification and classification of microorganisms. Despite many advances, microbial isolation and culture still face great challenges, including the fact that many microorganisms are difficult to culture under laboratory conditions. It is estimated that less than 1% of microorganisms can grow on traditional culture media. Different microorganisms have different requirements for culture conditions, including temperature, pH value, oxygen concentration, etc., which makes it very complicated and time-consuming to find suitable culture conditions for specific microorganisms. Efficient isolation and culture technology usually requires expensive equipment and reagents, which is a technical and economic barrier. However, pure culture of microorganisms can ensure the singleness and purity of the research object, thereby improving the accuracy and repeatability of experimental results. Through pure culture, researchers can gain a deep understanding of the physiological characteristics, metabolic pathways and ecological effects of specific microorganisms, which is helpful for the development of new drugs and industrial applications. Therefore, there is an urgent need for an economical and efficient microbial culture device to isolate a large number of microorganisms with application value from the environment.

[0003] Existing traditional microbial culture techniques have many shortcomings, mainly including low success rate, single isolated microbial species, susceptibility to cross-contamination, and high resource consumption. These shortcomings are mainly due to the fact that traditional culture techniques rely on a single culture medium and specific environmental conditions, which makes it difficult to meet the diverse growth needs of different microorganisms. In traditional culture techniques, most microorganisms cannot grow on a single culture medium, resulting in only a few microorganisms that can adapt to these conditions being successfully isolated. This not only limits our understanding of microbial diversity, but also leaves many microorganisms with potential application value undiscovered and unused. In addition, traditional techniques are susceptible to contamination by other microorganisms during the separation and culture process, resulting in reduced accuracy and reliability of experimental results. At the same time, in order to find suitable culture conditions, researchers need to conduct a large number of experiments, which is not only time-consuming and labor-intensive, but also requires a large amount of reagents and equipment, increasing research costs.

[0004] Therefore, in order to address the shortcomings of traditional microbial cultivation technology, such as low success rate, single separated species, susceptibility to contamination and high resource consumption, a gradient geochemical environment microbial enrichment and separation system is provided. Summary of the invention

[0005] The purpose of the present invention is to overcome the existing defects and provide a gradient geochemical environment microorganism enrichment and separation system, thereby improving the enrichment and separation efficiency of microorganisms.

[0006] The technical solution to achieve the above purpose is: A gradient geochemical environment microorganism enrichment and separation system, comprising: a glass barrel, a plurality of sampling ports are vertically arranged on the side wall of the glass barrel, a circulation inlet is arranged at the lower end of the side wall of the glass barrel, a circulation outlet is arranged at the upper end of the side wall of the glass barrel, and a feeding port is arranged at the upper end of the glass barrel; Also includes: A detection module for detecting geochemical and nutrient element characteristics of extracted microbial samples; A design module used to design corresponding solid or liquid culture media according to the detected geochemical and nutrient element characteristics for further separation and purification.

[0007] Preferably, the glass barrel is in a columnar form, and is used to carry environmental samples, culture fluid, and quartz sand or other fillers that are conducive to the survival and separation of microorganisms, thereby forming different geochemical gradient layers.

[0008] Preferably, different geochemical gradient layers include but are not limited to changes in pH value, oxygen concentration, and nutrient elements.

[0009] Preferably, the sampling port is used to extract microbial samples from different geochemical gradient layers in the glass barrel.

[0010] Preferably, a valve is provided at the sampling port.

[0011] Preferably, the circulation inlet is used to pass the culture solution into the glass barrel.

[0012] Preferably, the circulation outlet is used to discharge the culture solution out of the glass barrel.

[0013] Preferably, the feeding port is used to fill the glass barrel with environmental samples and quartz sand or other fillers that are conducive to the survival and separation of microorganisms.

[0014] The beneficial effects of the present invention are: The present invention can better simulate the complex conditions in the natural environment through the diversified geochemical gradients formed in the glass barrel, so that more types of microorganisms can grow under suitable conditions, thereby improving the success rate of cultivation; The present invention can enrich and separate a large number of different types of microorganisms by sampling at different gradient layers, thus overcoming the problem of separating a single species in traditional culture technology; The sampling port design of the present invention allows multiple sampling during the enrichment process, which helps to timely detect and control cross contamination and ensure the accuracy and reliability of the experimental results; The invention has a simple design and diversified culture conditions. Multiple microbial samples can be obtained through one experiment, which reduces a large number of repeated experiments and resource consumption and improves research efficiency. The present invention can more realistically reflect the survival status and mutual relationship of microorganisms in the natural environment by simulating the geochemical gradient conditions in the natural environment, and help to gain a deeper understanding of the role of microorganisms in the ecosystem; In summary, the present invention significantly improves the enrichment and separation efficiency of microorganisms by creating a diverse geochemical gradient environment, overcomes many shortcomings of traditional technologies, and provides a more effective tool for the study of microbial diversity and function. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a structural diagram of a gradient geochemical environment microorganism enrichment and separation system of the present invention; Figure 2 It is a module diagram of a gradient geochemical environment microorganism enrichment and separation system of the present invention.

[0016] In the figure: 1. Glass barrel; 2. Sampling port; 3. Circulation inlet; 4. Circulation outlet; 5. Feeding port; 6. Detection module; 7. Design module; 21. Valve. DETAILED DESCRIPTION

[0017] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. In the description of the present invention, it should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside" and the like indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings, which are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0018] The present invention will be further described below in conjunction with the accompanying drawings.

[0019] like Figure 1 As shown, a gradient geochemical environment microbial enrichment and separation system includes: a glass barrel 1, a plurality of sampling ports 2 are vertically arranged on the side wall of the glass barrel 1, a circulation inlet 3 is arranged at the lower end of the side wall of the glass barrel 1, a circulation outlet 4 is arranged at the upper end of the side wall of the glass barrel 1, and a feeding port 5 is arranged at the upper end of the glass barrel 1.

[0020] like Figure 2 As shown, a gradient geochemical environment microbial enrichment and separation system also includes: The detection module 6 is used to detect the geochemical and nutritional element characteristics of the extracted microbial samples.

[0021] The design module 7 is used to design corresponding solid or liquid culture medium according to the detected geochemical and nutrient element characteristics for further separation and purification.

[0022] In the embodiment, the glass barrel 1 is in a columnar form, which is used to carry environmental samples, culture medium, and quartz sand or other fillers that are conducive to the survival and separation of microorganisms, thereby forming different geochemical gradient layers. The different geochemical gradient layers include but are not limited to changes in pH value, oxygen concentration, and nutrients; it can better simulate the complex conditions in the natural environment, allowing more types of microorganisms to grow under suitable conditions, thereby improving the success rate of cultivation.

[0023] In the embodiment, the sampling port 2 is used to extract microbial samples from different geochemical gradient layers in the glass barrel 1; by sampling from different gradient layers, a large number of different types of microorganisms can be enriched and separated, overcoming the problem of separating a single species in traditional culture technology, and multiple sampling can be performed during the enrichment process, which helps to timely detect and control cross contamination and ensure the accuracy and reliability of experimental results. In addition, the device is simple in design and has diversified culture conditions. Multiple microbial samples can be obtained through one experiment, reducing a large number of repeated experiments and resource consumption, and improving research efficiency.

[0024] In the embodiment, a valve 21 is provided at the sampling port 2 .

[0025] In the embodiment, the circulation inlet 3 is used to pass the culture solution into the glass barrel 1.

[0026] In the embodiment, the circulation outlet 4 is used to discharge the culture solution from the glass barrel 1 .

[0027] In the embodiment, the feeding port 5 is used to fill the glass barrel 1 with environmental samples and quartz sand or other fillers that are helpful for the survival and separation of microorganisms.

[0028] The environmental sample is placed at the bottom of the glass barrel 1 through the feeding port 5, and quartz sand or other fillers that are conducive to the survival and separation of microorganisms are filled in it. Different culture solutions are gradually injected into the glass barrel 1 through the circulation inlet 3. These culture solutions will form a gradual geochemical gradient in the vertical direction inside the glass barrel 1, such as the gradient of pH, oxygen concentration and nutrients. After a period of cultivation, different types of microorganisms will be enriched in different layers of the glass barrel 1. Then, the microbial samples of each layer are taken out through the sampling port 2, and the geochemical and nutrient element characteristics of these layers are detected, and the corresponding solid or liquid culture medium is designed for further separation and purification. This process can not only significantly improve the enrichment and separation efficiency of microorganisms, but also maintain the diversity and ecological function of microorganisms to a greater extent.

[0029] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that the technical solutions described in the above embodiments may still be modified, or some or all of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A gradient geochemical environment microbial enrichment and separation system, characterized in that: include: A glass barrel (1), wherein a plurality of sampling ports (2) are vertically arranged on the side wall of the glass barrel (1), a circulation inlet (3) is arranged at the lower end of the side wall of the glass barrel (1), a circulation outlet (4) is arranged at the upper end of the side wall of the glass barrel (1), and a feeding port (5) is arranged at the upper end of the glass barrel (1); Also includes: A detection module for detecting the geochemical and nutrient characteristics of the extracted microbial samples (6); A design module for designing corresponding solid or liquid culture media based on the detected geochemical and nutrient element characteristics for further separation and purification (7).

2. A gradient geochemical environment microorganism enrichment and separation system according to claim 1, characterized in that: The glass barrel (1) is in a columnar form and is used to carry environmental samples, culture fluid, and quartz sand or other fillers that are conducive to the survival and separation of microorganisms, thereby forming different geochemical gradient layers.

3. A gradient geochemical environment microorganism enrichment and separation system according to claim 2, characterized in that: Different geochemical gradient layers include but are not limited to changes in pH, oxygen concentration, and nutrient elements.

4. A gradient geochemical environment microorganism enrichment and separation system according to claim 2, characterized in that: The sampling port (2) is used to extract microbial samples from different geochemical gradient layers in the glass barrel (1).

5. A gradient geochemical environment microorganism enrichment and separation system according to claim 3, characterized in that: The sampling port (2) is provided with a valve (21).

6. The gradient geochemical environment microorganism enrichment and separation system according to claim 1, characterized in that: The circulation inlet (3) is used to pass the culture solution into the glass barrel (1).

7. The gradient geochemical environment microorganism enrichment and separation system according to claim 1, characterized in that: The circulation outlet (4) is used to discharge the culture solution out of the glass barrel (1).

8. The gradient geochemical environment microorganism enrichment and separation system according to claim 1, characterized in that: The feeding port (5) is used to fill the glass barrel (1) with environmental samples and quartz sand or other fillers that are conducive to the survival and separation of microorganisms.

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