Traditional Chinese medicine decoction and intestinal probiotics combined medicine for treating ulcerative colitis

Abstract

The invention discloses a traditional Chinese medicine decoction and intestinal probiotic combined medicine for treating ulcerative colitis. The medicine is prepared by uniformly mixing traditional Chinese medicine decoction and intestinal probiotic liquid, the traditional Chinese medicine decoction is prepared by adding water into pulsatilla chinensis, cortex fraxini, coptis chinensis, radix sophorae flavescentis, hairyvein agrimony, pomegranate rind, purslane, sophora flower and sanguisorba officinalis, soaking at room temperature, decocting with strong fire, then slowly decocting with slow fire, and filtering. The intestinal probiotic liquid is one of clostridium butyricum liquid, lactobacillus plantarum liquid and lactobacillus reuteri liquid or a mixture of two or more than two of the clostridium butyricum liquid, the lactobacillus plantarum liquid and the lactobacillus reuteri liquid in equal volume. The traditional Chinese medicine is rich in raw materials, easy to produce and prepare, convenient to take and good in effect, provides a new medicine for treating ulcerative colitis, opens up a new way for treating ulcerative colitis, and has huge economic and social benefits.

Description

Technical Field

[0001] The invention relates to the field of medicine, in particular to a medicine of a Chinese medicinal decoction combined with intestinal probiotics for treating ulcerative colitis. Background Art

[0002] Ulcerative colitis (UC) is a chronic nonspecific intestinal inflammatory disease that mainly affects the rectum and colon. The cause of the disease is still unclear and may be related to multiple factors such as genetics, environment, and immunity. Patients often present with symptoms such as abdominal pain, diarrhea, mucus, pus and blood in the stool, and the disease recurs repeatedly, seriously affecting the quality of life of patients. It has been reported that Baitouweng Decoction (the formula is Baitouweng 15g, Coptis chinensis 6g, Phellodendron chinense 12g, and Qinpi 12g) has a therapeutic effect on ulcerative colitis. In addition, Clostridium butyricum, Lactobacillus plantarum, and Lactobacillus reuteri, three intestinal probiotics, have also been recorded in the literature for the prevention or treatment of animal ulcerative colitis. However, Chinese medicine has complex ingredients, multiple targets, and there are many types of probiotics. Therefore, it is not easy to effectively combine different Chinese medicines with different probiotics and use them to treat specific diseases. Although there are reports on the combined use of Chinese medicine and multiple types of intestinal probiotics to treat ulcerative colitis, it is only a theoretical discussion and has no real practical significance. In order to explore whether the combined use can achieve the effect of 1+1>2, the present invention is to develop a new drug for treating ulcerative colitis, mix Chinese medicine decoction with intestinal probiotics to prepare a new drug, open up a new path for the clinical treatment of ulcerative colitis, and effectively relieve the pain of patients, but there has been no public report so far. Summary of the invention

[0003] In view of the above situation, in order to overcome the deficiencies of the prior art, the purpose of the present invention is to provide a medicine for treating ulcerative colitis by combining a Chinese medicine soup with intestinal probiotics, which can effectively solve the problem of medication for ulcerative colitis.

[0004] The technical solution provided by the present invention is a medicine for treating ulcerative colitis by combining a Chinese medicine soup with intestinal probiotics, wherein the medicine is prepared by uniformly mixing a Chinese medicine soup and an intestinal probiotic solution in a volume ratio of 3.5-4.5:2.5-3.5;

[0005] The Chinese medicine soup is prepared by weight from the following medicine raw materials: 12g of Pulsatilla striata, 9g of Cortex Fraxini, 6g of Coptis chinensis, 6g of Sophora flavescens, 9g of Agrimoniae, 6g of Pomegranate peel, 9g of Portulaca oleracea, 6g of Sophora japonica and 6g of Radix Sanguisorbae. The preparation method is as follows: putting the medicine raw materials into a container, adding water to immerse them, soaking them at room temperature for 30 minutes, first decocting them with high heat, then decocting them with low heat for 20 to 30 minutes, filtering them, removing the medicine residues, and obtaining the Chinese medicine soup;

[0006] The intestinal probiotic solution is one of Clostridium butyricum solution, Lactobacillus plantarum solution, and Lactobacillus reuteri solution, or a mixture of two or more of them in equal volumes. The concentration of Clostridium butyricum solution is 1×10 8 CFU / ml; the concentration of Lactobacillus plantarum liquid is 16.7×10 9 CFU / ml; the concentration of Lactobacillus reuteri liquid is 3.33×10 9 CFU / ml.

[0007] The invention has abundant raw materials, is easy to produce and prepare, is convenient to take, has good effects, provides a new medicine for treating ulcerative colitis, opens up a new way for treating ulcerative colitis, and has huge economic and social benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0008] Figure 1 This is a graph showing the weight changes of mice in each group in the experiment of the present invention;

[0009] Figure 2 This is a graph showing the effects of intestinal probiotics and Chinese medicine on the changes in body weight of UC mice in the experiment of the present invention;

[0010] Figure 3 This is a graph showing the effects of intestinal probiotics and Chinese medicine on the DAI score of UC mice in the experiment of the present invention;

[0011] Figure 4 This is a diagram showing the effects of intestinal probiotics and Chinese medicine on organ indexes of UC mice in the experiment of the present invention;

[0012] Figure 5 This is the effect of intestinal probiotics and traditional Chinese medicine on the colon tissue of UC mice in the experiment of the present invention (HE×40, ×400, n=3) Legend: blue arrow: muscle layer; red star: goblet cell; red arrow: inflammatory cell; yellow arrow: lamina propria. DETAILED DESCRIPTION

[0013] The specific implementation modes of the present invention are described in detail below in combination with embodiments and specific situations.

[0014] Embodiment 1:

[0015] The invention discloses a medicine of Chinese medicinal decoction combined with intestinal probiotics for treating ulcerative colitis. The medicine is prepared by uniformly mixing the Chinese medicinal decoction and intestinal probiotics liquid in a volume ratio of 4:3.

[0016] The Chinese medicine soup is prepared by weight from the following medicine raw materials: 12g of Pulsatilla striata, 9g of Cortex Fraxini, 6g of Coptis chinensis, 6g of Sophora flavescens, 9g of Agrimoniae, 6g of Pomegranate peel, 9g of Portulaca oleracea, 6g of Sophora japonica and 6g of Radix Sanguisorbae. The preparation method is as follows: putting the medicine raw materials into a container, adding water to immerse them, soaking them at room temperature for 30 minutes, first decocting them with high heat, then decocting them with low heat for 20 to 30 minutes, filtering them, removing the medicine residues, and obtaining the Chinese medicine soup;

[0017] The intestinal probiotic solution is one of Clostridium butyricum solution, Lactobacillus plantarum solution, and Lactobacillus reuteri solution, or a mixture of two or more of them in equal volumes. The concentration of Clostridium butyricum solution is 1×10 8 CFU / ml; the concentration of Lactobacillus plantarum liquid is 16.7×10 9 CFU / ml; the concentration of Lactobacillus reuteri liquid is 3.33×10 9 CFU / ml.

[0018] Embodiment 2:

[0019] The invention discloses a medicine of Chinese medicinal decoction combined with intestinal probiotics for treating ulcerative colitis. The medicine is prepared by uniformly mixing the Chinese medicinal decoction and Clostridium butyricum liquid in a volume ratio of 3.5:2.5. The preparation method is the same as that in Example 1.

[0020] Embodiment 3:

[0021] The invention discloses a medicine of Chinese medicinal decoction combined with intestinal probiotics for treating ulcerative colitis. The medicine is prepared by uniformly mixing the Chinese medicinal decoction and Lactobacillus plantarum liquid in a volume ratio of 4.5:3.5. The preparation method is the same as that in Example 1.

[0022] Embodiment 4:

[0023] The invention discloses a medicine of Chinese medicinal decoction combined with intestinal probiotics for treating ulcerative colitis. The medicine is prepared by uniformly mixing the Chinese medicinal decoction and Lactobacillus reuteri liquid in a volume ratio of 3.5:3.5. The preparation method is the same as that in Example 1.

[0024] Example 5

[0025] The invention discloses a medicine of Chinese medicinal decoction combined with intestinal probiotics for treating ulcerative colitis. The medicine is prepared by uniformly mixing the Chinese medicinal decoction and a mixed bacterial liquid in a volume ratio of 4.5:2.5. The mixed bacterial liquid is a mixed bacterial liquid of equal volumes of Clostridium butyricum liquid and Lactobacillus plantarum liquid.

[0026] Example 6

[0027] The invention discloses a medicine of Chinese medicinal decoction combined with intestinal probiotics for treating ulcerative colitis. The medicine is prepared by uniformly mixing the Chinese medicinal decoction and a mixed bacterial liquid in a volume ratio of 4:3. The mixed bacterial liquid is a mixed bacteria of equal volumes of Clostridium butyricum liquid, Lactobacillus plantarum liquid and Lactobacillus reuteri liquid.

[0028] The medicine given in the above embodiments can be prepared in any amount as needed. The given embodiments are only used to illustrate the specific implementation mode of the present invention, rather than to limit the protection scope of the present invention. The technical core protected by the present invention is a new medicine composed of Chinese herbal medicine soup combined with intestinal probiotics.

[0029] Among the above-mentioned traditional Chinese medicines,

[0030] Pulsatilla, cold in nature, bitter in taste. Enters the stomach meridian and large intestine meridian. Clears away heat, detoxifies, cools blood and stops dysentery.

[0031] Qinpi, cold in nature, bitter and astringent in taste. Enters the liver meridian, gallbladder meridian, and large intestine meridian. Clears away heat and dampness, astringes, and improves eyesight.

[0032] Coptis chinensis is cold in nature and bitter in taste. It enters the heart meridian, spleen meridian, stomach meridian, liver meridian, gallbladder meridian, and large intestine meridian. It clears away heat and dampness, and purges fire and detoxifies.

[0033] Sophora flavescens, cold in nature, bitter in taste. It enters the heart meridian, liver meridian, stomach meridian, large intestine meridian, and bladder meridian. It clears away heat and dampness, kills insects, and is a diuretic.

[0034] Agrimony is neutral in nature, bitter and astringent in taste. It enters the heart meridian and liver meridian. It has the functions of astringent, hemostasis, antimalarial, antidysentery and detoxification.

[0035] Pomegranate peel is warm in nature, sour and astringent in taste. It enters the large intestine meridian. It can astringe the intestines, stop diarrhea, stop bleeding, and expel parasites.

[0036] Purslane is sour and cold in nature. It enters the large intestine and liver meridians. It clears away heat and detoxifies, cools blood and stops bleeding.

[0037] Sophora japonica flower, slightly cold in nature, bitter in taste, enters the liver meridian and large intestine meridian, cools blood and stops bleeding, clears the liver and purges fire.

[0038] Radix Sanguisorbae, slightly cold in nature, bitter, sour and astringent in taste. It enters the liver meridian and large intestine meridian. It cools blood and stops bleeding, detoxifies and heals sores.

[0039] The Chinese medicine is based on the principles of traditional Chinese medicine, according to the main and secondary monarchs, ministers, assistants and messengers, and is functionally related to each other and works in combination with each other. In the Chinese medicine prescription, Pulsatilla striata is the monarch, Coptis chinensis is the minister, Sophora flavescens, Agrimoniae herba, Pomegranate peel, Portulaca oleracea, Sophora japonica and Sanguisorba officinalis are adjuvants, and Fraxinus chinensis is the messenger. All the medicines work together to work together, and have the effects of anti-inflammatory, analgesic, detoxification and hemostasis. Combined with intestinal probiotics, it can be effectively used to treat ulcerative colitis and reduce the side effects of Western medicine 5-aminosalicylic acid, and has achieved very good beneficial technical effects through experiments. The relevant information is as follows (taking Example 1 as an example):

[0040] 1. Experimental Methods

[0041] 1.1 Experimental grouping, modeling and drug administration

[0042] After one week of adaptive feeding, the animals were randomly divided into a blank group, a model group, a positive drug group (5-ASA), a Clostridium butyricum group, a Lactobacillus plantarum group, a Lactobacillus reuteri group, a Chinese medicine group, a bacteria combination group, and a bacteria-drug combination group. Except for the blank group, the mice in the other groups were allowed to freely drink 3% DSS (dextran sulfate sodium) solution for 7 days to induce an acute ulcerative colitis (UC) model. After the model was successfully established, the mice were weighed at 9 am every day and then given oral gavage.

[0043] The blank group and the model group were given normal saline (0.2 ml / 10 g);

[0044] Positive drug group (5-ASA, 0.2ml / 10g, 40mg / kg);

[0045] Clostridium butyricum group (1×10 8 CFU / ml, 0.15ml / 10g);

[0046] Lactobacillus plantarum group (16.7×10 9 CFU / ml, 0.15ml / 10g);

[0047] Lactobacillus reuteri group (3.33×10 9 CFU / ml, 0.15ml / 10g);

[0048] Chinese medicine group (0.2ml / 10g);

[0049] Bacteria combination group (three bacterial solutions were mixed evenly in equal volumes, 0.15 ml / 10 g);

[0050] Bacteria and medicine combined group (4 ml of Chinese medicine solution and 3 ml of bacteria mixture were mixed evenly, 0.15 ml / 10 g);

[0051] Administer once a day for 7 consecutive days.

[0052] 1.2 Mouse body weight measurement and disease activity index scoring

[0053] During the experiment, the weight of mice was measured at the same time every day, and the stool morphology and degree of blood in the stool were observed and recorded. Starting from the second day of the experiment, the disease activity index (DAI) was scored based on the percentage of weight loss, stool characteristics and degree of blood in the stool of mice. DAI = (weight loss rate score + stool characteristics score + degree of blood in the stool score) / 3, and the DAI scoring standard is shown in Table 3. The fecal occult blood qualitative kit (Pirami method) was used to detect the occult blood in the stool of mice, and the judgment criteria are shown in Table 4.

[0054] Table 3 DAI scoring criteria

[0055]

[0056] Table 4 Criteria for determining occult blood in mouse feces

[0057]

[0058] 1.3 Sample collection and indicator determination

[0059] After the experiment, mice were fasted but not watered for 8 hours. The last dose was given, and 2 hours later, the eyeballs of mice were removed to collect blood, placed on ice for 30 minutes, centrifuged at 3500r / min for 15 minutes, and serum was collected and stored in a -80℃ refrigerator for later use. The mice were killed by cervical dislocation and the colon tissue was dissected and separated. Three mice were selected from each group to cut 1cm of colon tissue from the same position, fixed in 4% paraformaldehyde, and the pathological changes of colon tissue were observed by HE staining. The spleen and liver tissues were weighed and the organ coefficient was calculated. The organ index (mg / g) = organ mass (mg) / mouse body mass (g) × 100%. All samples were extracted in liquid nitrogen for 10 minutes and quickly transferred to a -80℃ refrigerator for later use.

[0060] 1.4 Observation of pathological morphological changes of colon tissue by HE staining

[0061] After the mouse colon tissue was fixed in 4% paraformaldehyde solution for 120 hours, the tissue was trimmed and dehydrated. The tissue sample was then placed in dissolved paraffin, embedded and cooled into blocks, and the wax block was cut into slices of about 5 μm thick using a microtome. The slices were placed in xylene I and xylene II for 20 minutes each, then placed in anhydrous ethanol I, anhydrous ethanol II for 5 minutes and 75% ethanol for 5 minutes each, and then rinsed with tap water. For hematoxylin staining, the slices were placed in hematoxylin staining solution for 3-5 minutes, then rinsed with tap water, differentiated with differentiation solution, rinsed with tap water, blued with blueing solution, and then rinsed with running water. Then, eosin staining was performed, and the slices were placed in 85% and 95% gradient ethanol solutions for dehydration for 5 minutes each, and then placed in eosin staining solution for 5 minutes. Finally, dehydrate and seal the slices, place the slices in anhydrous ethanol I, anhydrous ethanol II, and anhydrous ethanol III for 5 minutes each, and then place them in xylene I and xylene II for 5 minutes each until the slices are transparent, and seal the slices with sealing glue. Examine under a microscope and take pictures.

[0062] 1.5 Data Statistical Methods

[0063] GraphPad Prism 5.0 software was used for drawing, and SPSS25.0 software was used for statistical analysis of experimental data. One-way ANOVA analysis was used when the experimental data conformed to normal distribution. If the variance was equal, the LSD method was used for inter-group comparison. If the variance was unequal, the Dunnett's T3 method was used for inter-group comparison. P<0.05 was considered to be statistically significant, and the results were expressed as "mean ± standard deviation"

[0064] 2. Experimental results

[0065] 2.1 Effects of bacteria and Chinese medicine on body weight and daily changes in UC mice

[0066] The mice in the blank group ate and drank normally throughout the experimental period, had smooth hair, normal stool characteristics, were in good mental state, and gradually gained weight.

[0067] The food intake of mice in the model group, positive drug group (5-ASA), Clostridium butyricum group, Lactobacillus plantarum group, Lactobacillus reuteri group, Chinese medicine group, bacteria combination group and bacteria-drug combination group was significantly reduced. Loose stools were observed on the third day of modeling, and diarrhea, bloody stools, and anal blood staining occurred to varying degrees on the 5th and 6th days. The hair of most mice lost its luster and their spirits became listless. It was preliminarily judged that the modeling was successful.

[0068] After 3 days of drug treatment, except for the DSS group, the conditions of the mice in the other 7 groups improved, their food intake gradually increased, and their stool characteristics gradually improved. After 1 week of drug administration, compared with the blank group, the body weight of the model group decreased significantly (P<0.01); compared with the model group, the body weights of the positive drug group, Lactobacillus reuteri group, combined bacteria group, Chinese medicine group, and combined bacteria and medicine group increased significantly (P<0.01); the body weights of the Clostridium butyricum and Lactobacillus plantarum groups increased significantly (P<0.05). Although there was no significant difference in the average body weights of the drug groups, there was a trend of combined bacteria and medicine group > Lactobacillus reuteri group > combined bacteria group > positive drug group > Chinese medicine group > Lactobacillus plantarum group > Clostridium butyricum group. It can be seen that DSS as a modeling drug can cause a decrease in the body weight of mice, and treatment with single bacteria, single Chinese medicine, or combined bacteria and medicine can restore their body weight, and the combined bacteria and medicine group has the best therapeutic effect, which is closest to the blank group. Figure 1 .

[0069] 2.2 Effects of bacteria and Chinese medicine on body weight changes in UC mice

[0070] The results of one-way ANOVA showed that the body weight of mice in different groups (F 8,40 =0.136, P<0.01) showed significant difference.

[0071] The results of multiple comparisons (LSD) showed that compared with the blank group, the body weight of the model group was significantly reduced (P<0.01); compared with the model group, the body weight of the positive drug group, Clostridium butyricum, Lactobacillus plantarum group, Lactobacillus reuteri group, bacteria combination group, Chinese medicine group and bacteria-drug combination group were significantly increased (P<0.01). In addition, although there was no significant difference in the changes in body weight among the drug-administered groups, there was a trend of positive drug group> bacteria-drug combination group> Lactobacillus reuteri group> Chinese medicine group> bacteria combination group> Lactobacillus plantarum group> Clostridium butyricum group, and overall, the effect of the bacteria-drug combination group was better than that of other drugs except the positive drug. Figure 2 .

[0072] 2.3 Effects of bacteria and Chinese medicine on DAI scores of UC mice

[0073] The DAI score is an important indicator for evaluating colitis. The score can reflect the severity of colitis in model animals, and the higher the score, the more severe the disease. In this experiment, compared with the blank group, the DAI score of the model group was significantly increased (P<0.01); compared with the model group, the DAI scores of the positive drug group, the Chinese medicine group and the combined bacteria and drug group were all significantly decreased (P<0.01), and the DAI score of the combined bacteria group also decreased significantly (P<0.05). Although the DAI scores of the Clostridium butyricum, Lactobacillus plantarum and Lactobacillus reuteri groups were not significantly different from those of the model group (P>0.05), the scores showed a downward trend, as shown in Figure 2. Figure 3 .

[0074] 2.4 Effects of bacteria and Chinese medicine on organ indexes of UC mice

[0075] Compared with the blank group, the spleen and liver organ indexes of the mice in the model group after DSS induction were significantly increased (P<0.01); compared with the model group, the spleen and liver organ indexes of the mice in the positive drug group, the bacteria combination group, the Chinese medicine group and the bacteria and medicine combination group were significantly decreased (P<0.01); the spleen index of the mice in the Clostridium butyricum group was significantly reduced (P<0.05), and the liver index of Lactobacillus reuteri was significantly reduced (P<0.05). In general, the combined use of bacteria and medicine has the best effect, which can restore the spleen and liver indexes of mice, improve the body's immunity, and strengthen the body's ability to prevent and resist diseases. Figure 4 .

[0076] 2.5 Effects of bacteria and Chinese medicine on colon tissue of UC mice

[0077] The colonic glands of the blank group mice were arranged neatly and orderly, the mucosal epithelial tissue was intact, the mucus layer was smooth, and the crypt structure was intact. The colonic mucosal epithelial cells and crypt structure of the model group mice were destroyed, the glands of the lamina propria fell off, the arrangement was disordered, the degree of atrophy and deformation was serious, the number of goblet cells decreased, the arrangement was disordered and the morphological structure was destroyed, and the inflammatory cell infiltration was serious. The colon mucosal layer and crypt structure of the mice in each drug-treated group tended to be complete, the number of lamina propria glands increased, the degree of atrophy and deformation decreased, and the arrangement was orderly. Not only was there no obvious inflammatory cell infiltration, but the number and morphology of goblet cells were also restored. The combined administration of bacteria and drugs had the best therapeutic effect, with a significantly increased number of goblet cells and the best morphological recovery. It can be seen that the advantages of the combined use of traditional Chinese medicine and intestinal probiotics are not only that it can increase the efficacy of a single traditional Chinese medicine or a single bacteria, but also that it has fewer side effects and better overall efficacy than the positive drug 5-ASA, as shown in the figure. Figure 5 .

[0078] While conducting experiments on Example 1, the same experiments were conducted on other examples respectively, and the same or similar results were obtained, which will not be listed one by one.

[0079] 3. Conclusion

[0080] The results of body weight and body mass showed that after treatment with Chinese herbal decoction alone, the weight of mice increased significantly, with a body mass increase of 12.85%; after treatment with bacterial liquid alone, the weight of mice also increased, with a body mass increase of 11.93%; and after treatment with Chinese herbal decoction combined with bacterial liquid, the weight increased extremely significantly, with a body mass increase of 15.03%. It can be seen that the combined use of bacteria and medicine has the effect of "1+1>2".

[0081] The DAI score results showed that the DAI score decreased by 2.67 points after treatment with Chinese herbal decoction alone, by 2.2 points after treatment with bacterial solution, and by 2.68 points after combined treatment with bacterial and drug. It can be seen that the combined use of bacterial and drug is more effective in reducing the DAI score, showing a quantitative advantage of "1+1>2".

[0082] The results of organ index showed that the organ indexes of spleen and liver of mice were 4.56mg / g and 5.69mg / g respectively after treatment with Chinese herbal decoction alone, 4.58mg / g and 5.93mg / g respectively after treatment with bacterial solution, and 4.51mg / g and 5.67mg / g respectively after combined treatment with bacterial and drug. It can be seen that combined treatment with bacterial and drug has a more significant effect in reducing the organ indexes of spleen and liver of mice.

[0083] In addition, experiments have shown that the Chinese herbal soup of the present invention can improve the overall physical condition of the patient, which creates a better internal environment for the bacterial liquid to exert its effect; and research on the bacterial liquid has found that it can enhance intestinal function and further promote the absorption and utilization of the Chinese herbal soup of the present invention. The two can be used together to exert a better therapeutic effect.

[0084] In summary, the combined use of the Chinese herbal medicine decoction and the three bacterial liquids of the present invention is superior to the single use of Chinese herbal medicine or the single use of bacterial liquid in terms of overall levels such as body weight, body mass, DAI score and organ index, as well as in the HE staining pathological level, showing the technical effect of "1+1>2", highlighting the technical progress and creative value of the present invention.

Claims

1. A medicine for treating ulcerative colitis comprising a Chinese medicine soup combined with intestinal probiotics, the medicine being prepared by uniformly mixing the Chinese medicine soup and intestinal probiotics liquid in a volume ratio of 3.5-4.5:2.5-3.5; The Chinese medicine soup is prepared by weight from the following medicine raw materials: 12g of Pulsatilla striata, 9g of Cortex Fraxini, 6g of Coptis chinensis, 6g of Sophora flavescens, 9g of Agrimoniae, 6g of Pomegranate peel, 9g of Portulaca oleracea, 6g of Sophora japonica and 6g of Radix Sanguisorbae. The preparation method is as follows: putting the medicine raw materials into a container, adding water to immerse them, soaking them at room temperature for 30 minutes, first decocting them with high heat, then decocting them with low heat for 20 to 30 minutes, filtering them, removing the medicine residues, and obtaining the Chinese medicine soup; The intestinal probiotic solution is one of Clostridium butyricum solution, Lactobacillus plantarum solution, and Lactobacillus reuteri solution, or a mixture of two or more of them in equal volumes. The concentration of Clostridium butyricum solution is 1×10 8 CFU / ml; the concentration of Lactobacillus plantarum liquid is 16.7×10 9 CFU / ml; the concentration of Lactobacillus reuteri liquid is 3.33×10 9 CFU / ml.

2. The Chinese medicine decoction combined with intestinal probiotics for treating ulcerative colitis according to claim 1, characterized in that: The medicine is prepared by uniformly mixing a Chinese medicine soup and an intestinal probiotic liquid in a volume ratio of 4:

3.

3. The medicine for treating ulcerative colitis by combining a Chinese medicinal decoction with intestinal probiotics according to claim 1, characterized in that: The medicine is prepared by uniformly mixing a traditional Chinese medicine soup and a Clostridium butyricum liquid in a volume ratio of 3.5:2.

5.

4. The medicine for treating ulcerative colitis by combining a Chinese medicinal decoction with intestinal probiotics according to claim 1, characterized in that: The medicine is prepared by uniformly mixing a Chinese medicinal decoction and a Lactobacillus plantarum liquid in a volume ratio of 4.5:3.

5.

5. The Chinese medicine decoction combined with intestinal probiotics for treating ulcerative colitis according to claim 1, characterized in that: The medicine is prepared by uniformly mixing a traditional Chinese medicine soup and a Lactobacillus reuteri liquid in a volume ratio of 3.5:3.

5.

6. The Chinese medicine decoction combined with intestinal probiotics for treating ulcerative colitis according to claim 1, characterized in that: The medicine is prepared by uniformly mixing a traditional Chinese medicine soup and a mixed bacterial liquid in a volume ratio of 4.5:2.5, and the mixed bacterial liquid is a mixed bacterial liquid of equal volumes of Clostridium butyricum liquid and Lactobacillus plantarum liquid.

7. The Chinese medicine decoction combined with intestinal probiotics for treating ulcerative colitis according to claim 1, characterized in that: The medicine is prepared by uniformly mixing a Chinese medicine soup and a mixed bacterial liquid in a volume ratio of 4:3, and the mixed bacterial liquid is a mixed bacterial liquid of equal volumes of Clostridium butyricum liquid, Lactobacillus plantarum liquid and Lactobacillus reuteri liquid.

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