Peptide-containing composition for improving immunity of human body and preparation method of peptide-containing composition

Abstract

The invention relates to the technical field of medicines, and discloses a peptide-containing composition for improving human immunity and a preparation method thereof. The peptide-containing composition is prepared by mixing the following components: an active polypeptide liposome, an antioxidant, a sweetening agent and a flocculating agent according to a ratio of (8-25): (1-3): (2-4): 1, so as to obtain the peptide-containing composition, wherein the active polypeptide liposome, the antioxidant, the sweetening agent and the flocculating agent are mixed according to a ratio of (8-25): (1-3): (2-4): 1. The active polypeptide liposome is granular and has a double-layer embedding structure; the active polypeptide liposome comprises the following components in percentage by mass: 60%-80% of polypeptide concentrated solution, 10%-40% of amino acid, 5%-30% of vitamin and 5%-18% of calcium. The peptide-containing composition is an oral liquid preparation and is good in palatability, polypeptide active ingredients are not affected by stomach digestion and can be fully released in small intestines, the absorbance of the polypeptide active ingredients in a human body is improved, and therefore the health care effect is enhanced.

Description

Technical Field

[0001] The invention relates to the field of medical technology, in particular to a peptide-containing composition for improving human immunity, and also to a preparation method of the peptide-containing composition. Background Art

[0002] Polypeptide is a compound formed by α-amino acids linked together by peptide bonds. It is also an intermediate product of protein hydrolysis. The compound formed by the dehydration condensation of two amino acid molecules is called a dipeptide. Similarly, there are tripeptides, tetrapeptides, pentapeptides, etc. Generally, the compound formed by the dehydration condensation of three or more amino acid molecules can be called a polypeptide.

[0003] Usually, peptide immune agents belong to polypeptide hormones, mainly drugs, such as thymosin, thymopentin, immunoglobulin, immunoglobulin G, human serum albumin, interferon, tumor cell necrosis factor, interleukin I, interleukin II, interleukin III, etc. These peptide immune agents are separated and extracted from animal glands, tissues and blood, and can only be used for patients with certain symptoms. During use, rejection, allergies and side effects are prone to occur, so they must be used with caution and must be used under the strict supervision of a doctor.

[0004] After oral peptide preparations are taken by the human body, they first pass through the digestive tract and then into the stomach. This process is affected by enzymes, gastric acid, etc., causing the polypeptide components to be oxidized and hydrolyzed, which affects the body's absorption and leads to a reduction in effectiveness. Summary of the invention

[0005] In view of the above-mentioned shortcomings of the prior art, the purpose of the present invention is to provide a peptide-containing composition for improving human immunity and a preparation method thereof to prepare a peptide-containing oral preparation with high absorption of polypeptide active ingredients in the human body and good palatability.

[0006] A peptide-containing composition for improving human immunity, the peptide-containing composition comprising the following components: active polypeptide liposomes, antioxidants, sweeteners and flocculants are mixed in a ratio of 8-25:1-3:2-4:1 to obtain the peptide-containing composition; The active polypeptide liposome is in granular form and has a double-layer embedding structure; the mass composition of the active polypeptide liposome is as follows: 60% to 80% of polypeptide concentrate, 10% to 40% of amino acids, 5% to 30% of vitamins, and 5% to 18% of calcium.

[0007] As a further improvement of the above scheme, the antioxidant is any one of sodium sulfite, sodium bisulfite, sodium pyrosulfite and vitamin C; the sweetener is any one of sucrose, stevioside, glycyrrhizin, syrup and Tiandongtian dipeptide; the flocculant is any one of citrate, hydrogen citrate, tartrate, hydrogen tartrate and phosphate.

[0008] As a further improvement of the above scheme, the amino acids include glutamic acid, lysine, threonine, cystine and valine. In the composition of the present invention, amino acid components are added to assist in improving immunity, specifically: glutamic acid helps human body signal conduction, which is beneficial to promoting immune cell activity, lysine helps to increase lymphocyte proliferation and antibodies, improves the resistance of the immune system, threonine is beneficial to enhance the activity of immune cells, and then helps the human body improve the function of the immune system, cystine helps the human body synthesize glutathione, can participate in the body's antioxidant reaction, and help regulate the immune system, and valine helps to enhance the activity of human immune cells and improve the body's defense against diseases.

[0009] As a further improvement of the above scheme, the vitamins include vitamin A, vitamin C, vitamin D, vitamin E, vitamin B1, vitamin B2, vitamin B6 and vitamin B12. In the composition of the present invention, vitamin ingredients are added to assist in improving immunity, specifically: vitamin A promotes cell growth and plays a role in protecting the body, vitamin C promotes cell metabolism and enhances the body's immunity, vitamin D promotes the body's absorption of calcium and phosphorus, and can also play a role in lung repair and immunosuppression, enhance grassroots immunity, vitamin E enhances the body's immunity and improves the body's antioxidant capacity, vitamin B1 combines with phosphate in the human body to become a coenzyme of pyruvate oxidative decarboxylase, and participates in the metabolism of pyruvate, vitamin B2 combines with specific proteins to form flavin enzymes, and flavin enzymes participate in a variety of redox reactions in the human body, vitamin B6 participates in the synthesis of antibodies, the manufacture of gastric acid in the digestive system, the utilization of fat and protein, and the maintenance of sodium / potassium balance, and vitamin B12 participates in the synthesis of cell nucleic acids and fatty acids.

[0010] A method for preparing a peptide-containing composition comprises the following steps: S1: protein is hydrolyzed to obtain a peptide concentrate; S2 Take the polypeptide concentrate, amino acids, vitamins and calcium according to the mass ratio, and mix them to obtain a mixed solution; S3 uses supercritical CO2 method to treat soybean lecithin and mixed solution to obtain liposome solution; S4 filters the liposome solution and retains the liposome particles; S5: performing embedding treatment on the liposome particles to obtain active polypeptide liposomes with double-layer coating; S6: taking active polypeptide liposomes, antioxidants, sweeteners and flocculants in proportion, adding water and stirring to mix, so as to obtain an oral liquid containing a peptide composition.

[0011] As a further improvement of the above scheme, the protein source is selected from meat, eggs or milk, and the enzymatic hydrolysis uses a composite enzyme obtained by mixing papain, ficin and bromelain in a ratio of 1:2:3. The present invention uses a composite enzyme to form a step-by-step enzymatic hydrolysis system, thereby improving the enzymatic hydrolysis efficiency of protein.

[0012] As a further improvement of the above scheme, the method for obtaining the polypeptide by enzymatic hydrolysis is specifically performed as follows: S11 takes protein powder made from protein and puts it into the fermentation tank. After adding water to S12, gradually heat the temperature to 40°C, and then add the complex enzyme for enzymolysis; S13 cools and filters to obtain a polypeptide concentrate.

[0013] As a further improvement of the above scheme, the method for treating soybean lecithin and mixed liquid by supercritical CO2 method is specifically operated as follows: S31 Take soybean lecithin and the mixed solution in a ratio of 7:1, mix and grind; S32 was added three times, 10 mL of 0.2 mol / L PBS phosphate buffer solution with a pH of 6.0 was added each time, and the mixture was further ground evenly, and then transferred to an autoclave; S33: At a shear rate of 3000 r / min and a temperature of 45°C, supercritical CO2 was introduced into the autoclave until the set pressure was reached, and then ultrasound was turned on with an ultrasound power of 18 W. The ultrasound and shearing treatment was maintained for 50 min, and the heating, shearing and ultrasound were stopped in sequence, and CO2 was released; S34 adds 100 mL of PBS phosphate buffer solution to wash the particles on the inner wall of the autoclave, and treats with ultrasound and stirring for 40 minutes to obtain a liposome solution. The present invention treats soybean lecithin and the mixed solution based on the supercritical CO2 method, so that the polypeptide component is encapsulated in the liposome.

[0014] As a further improvement of the above scheme, the method for obtaining active polypeptide liposomes with double-layer coating by embedding treatment is specifically performed as follows: S51: taking an enteric material and water in a volume ratio of 1:1, heating and ultrasonically stirring to obtain a solution; S52: dispersing liposome particles in the solution, mixing and loading into a spraying device, and controlling the size, speed and morphology of the piezoelectric spray head droplets according to preset parameters to obtain liposome particles with a coating layer; S53: polyvinyl alcohol and water in a volume ratio of 1:1, heating and ultrasonically stirring to obtain a polyvinyl alcohol aqueous solution; S54: dispersing the liposome particles obtained by one injection in the polyvinyl alcohol aqueous solution, mixing and loading into the injection device, and controlling the size, speed and morphology of the piezoelectric nozzle droplets according to preset parameters to obtain active polypeptide liposomes.

[0015] As a further improvement of the above scheme, the enteric material adopts any one of cellulose acetate phthalate, hydroxypropyl methylcellulose phthalate, polyvinyl phthalate and acrylic resin. In the present invention, polyvinyl alcohol is used as the outer coating to play a stabilizing and waterproofing role. In the present invention, the liposome particles are coated with the enteric material as the inner coating, and the enteric material has the characteristics of being insoluble in the stomach but soluble in the intestinal fluid, so that the active ingredients of the polypeptide are not affected by gastric digestion, and are fully released in the small intestine, thereby improving the absorption of the active ingredients of the polypeptide in the human body, thereby enhancing the health care effect.

[0016] Compared with the prior art, the present invention has the following beneficial effects: The peptide-containing composition of the present invention is an oral liquid preparation, wherein the active polypeptide liposomes have multiple active ingredients and can improve human immunity, the added antioxidant plays a role in preventing the effective ingredients in the oral liquid from being oxidized and prolonging the shelf life, the flocculant is added to the oral liquid so that the product can be restored to a uniformly dispersed suspension after shaking to avoid sedimentation for easy taking, and the sweetener plays a role in sweetening, so as to improve the palatability of the peptide-containing composition.

[0017] The present invention adopts a double-layer encapsulation preparation process to prepare active polypeptide liposomes, so that the active polypeptide liposomes have an inner coating and an outer coating. Enteric materials are used to prepare the inner layer to encapsulate liposome particles. The enteric materials have the characteristics of being insoluble in the stomach but soluble in intestinal fluid. Polyvinyl alcohol is used as the outer coating to play a stabilizing and waterproofing role, so that the active ingredients of the polypeptide are not affected by gastric digestion, and are fully released in the small intestine, thereby improving the absorbability of the active ingredients of the polypeptide in the human body, thereby enhancing the health care effect. DETAILED DESCRIPTION

[0018] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention is further described in detail below in conjunction with the embodiments. Additional aspects and advantages of the present invention will be given in part in the following description, and will become apparent from the following description, or will be understood through the practice of the present invention. It should be understood that the following description is only used to explain the present invention and is not intended to limit the present invention.

[0019] The specific embodiments of the present invention are described in detail below.

[0020] Example 1 This embodiment provides a peptide-containing composition for improving human immunity, which has the following components: active polypeptide liposomes, sodium sulfite, sucrose and tartrate are mixed in a ratio of 8:1:2:1 to obtain the peptide-containing composition. The active polypeptide liposomes are granular and have a double-layer embedding structure. The mass fraction of the active polypeptide liposomes is as follows: 60% polypeptide concentrate, 10% amino acids, 20% vitamins, and 10% calcium. The amino acids include glutamic acid, lysine, threonine, cystine and valine. The vitamins include vitamin A, vitamin C, vitamin D, vitamin E, vitamin B1, vitamin B2, vitamin B6 and vitamin B12.

[0021] The preparation method of the peptide-containing composition of this embodiment comprises the following steps: S1: enzymatically hydrolyze the protein to obtain a polypeptide concentrate, wherein the enzymatic hydrolysis uses a composite enzyme obtained by mixing papain, ficin and bromelain in a ratio of 1:2:3; The method for obtaining the polypeptide by enzymatic hydrolysis is specifically performed as follows: S11 takes the protein powder made from eggs and puts it into a fermentation tank; The volume ratio of S12 water and protein powder is 10:1. After adding water, the temperature is gradually raised to 40°C, and the complex enzyme is added for enzymolysis. The enzymolysis temperature is 40°C and the enzymolysis time is 60 minutes. The liquid after S13 enzymatic hydrolysis was barreled, cooled to 12°C, placed in a refrigerator for precipitation for 2 days, and then filtered to obtain a polypeptide concentrate.

[0022] S2: Take 60% of polypeptide concentrate, 10% of amino acids, 20% of vitamins and 10% of calcium in proportion by mass and mix them to obtain a mixed solution.

[0023] S3 uses supercritical CO2 method to treat soybean lecithin and mixed solution to obtain liposome solution.

[0024] The method for treating soybean lecithin and mixed liquid by supercritical CO2 method, the specific operation is as follows: S31 Take soybean lecithin and the mixed liquid in a ratio of 7:1, mix and grind.

[0025] S32 was added three times, 10 mL of 0.2 mol / L PBS phosphate buffer solution with a pH of 6.0 was added each time, and the mixture was further ground evenly, and then transferred to an autoclave.

[0026] S33: At a shear rate of 3000 r / min and a temperature of 45°C, supercritical CO2 was introduced into the autoclave until the set pressure was reached, and then ultrasound was turned on with an ultrasonic power of 18 W. The ultrasound and shearing treatments were maintained for 50 minutes. After stopping heating, shearing and ultrasound in turn, CO2 was released.

[0027] S34 added 100 mL of PBS phosphate buffer solution to rinse the particles on the inner wall of the autoclave, and treated with ultrasound and stirring for 40 minutes to obtain a liposome solution.

[0028] S4 filters the liposome solution and retains the liposome particles.

[0029] S5: embedding the liposome particles to obtain active polypeptide liposomes with double-layer coating.

[0030] The method for obtaining active polypeptide liposomes with double-layer coating by embedding treatment is specifically performed as follows: S51: Take cellulose acetate phthalate and water in a volume ratio of 1:1, heat and ultrasonically stir to obtain a solution.

[0031] S52: dispersing liposome particles in the solution, mixing and loading into a spraying device, controlling the size, speed and morphology of the piezoelectric spray head droplets according to preset parameters, and obtaining liposome particles with a coating layer.

[0032] S53: polyvinyl alcohol and water are mixed in a volume ratio of 1:1, heated and ultrasonically stirred to obtain a polyvinyl alcohol aqueous solution.

[0033] S54: dispersing the liposome particles obtained by one injection in the polyvinyl alcohol aqueous solution, mixing and loading into the injection device, and controlling the size, speed and morphology of the piezoelectric nozzle droplets according to preset parameters to obtain active polypeptide liposomes.

[0034] S6: Active polypeptide liposomes, sodium sulfite, sucrose and tartrate are taken in a ratio of 8:1:2:1, water is added and stirred to obtain an oral liquid containing a peptide composition. The oral liquid containing a peptide composition is sterilized at 100° C. and then filled and put on the market.

[0035] Example 2 This embodiment provides a peptide-containing composition for improving human immunity, which has the following components: active polypeptide liposomes, sodium sulfite, sucrose and tartrate are mixed in a ratio of 25:2:3:1 to obtain the peptide-containing composition. The active polypeptide liposomes are granular and have a double-layer embedding structure. The mass fraction of the active polypeptide liposomes is as follows: 70% polypeptide concentrate, 10% amino acids, 12% vitamins, and 8% calcium. The amino acids include glutamic acid, lysine, threonine, cystine and valine. The vitamins include vitamin A, vitamin C, vitamin D, vitamin E, vitamin B1, vitamin B2, vitamin B6 and vitamin B12.

[0036] The preparation method of the peptide-containing composition of this embodiment comprises the following steps: S1: enzymatically hydrolyze the protein to obtain a polypeptide concentrate, wherein the enzymatic hydrolysis uses a composite enzyme obtained by mixing papain, ficin and bromelain in a ratio of 1:2:3; The method for obtaining the polypeptide by enzymatic hydrolysis is specifically performed as follows: S11 takes the protein powder made from eggs and puts it into a fermentation tank; The volume ratio of S12 water to protein powder is 10:1. After adding water, the temperature is gradually raised to 40°C, and the complex enzyme is added for enzymolysis. The enzymolysis temperature is 40°C and the enzymolysis time is 60 minutes. The liquid after S13 enzymatic hydrolysis was barreled, cooled to 12°C, placed in a refrigerator for precipitation for 2 days, and then filtered to obtain a polypeptide concentrate.

[0037] S2: taking the polypeptide concentrate, amino acids, vitamins and calcium according to the mass ratio, and mixing them to obtain a mixed solution.

[0038] S3 uses supercritical CO2 method to treat soybean lecithin and mixed solution to obtain liposome solution.

[0039] The method for treating soybean lecithin and mixed liquid by supercritical CO2 method, the specific operation is as follows: S31 Take soybean lecithin and the mixed liquid in a ratio of 7:1, mix and grind.

[0040] S32 was added three times, 10 mL of 0.2 mol / L PBS phosphate buffer solution with a pH of 6.0 was added each time, and the mixture was further ground evenly, and then transferred to an autoclave.

[0041] S33: At a shear rate of 3000 r / min and a temperature of 45°C, supercritical CO2 was introduced into the autoclave until the set pressure was reached, and then ultrasound was turned on with an ultrasonic power of 18 W. The ultrasound and shearing treatments were maintained for 50 minutes. After stopping heating, shearing and ultrasound in turn, CO2 was released.

[0042] S34 added 100 mL of PBS phosphate buffer solution to rinse the particles on the inner wall of the autoclave, and treated with ultrasound and stirring for 40 minutes to obtain a liposome solution.

[0043] S4 filters the liposome solution and retains the liposome particles.

[0044] S5: embedding the liposome particles to obtain active polypeptide liposomes with double-layer coating.

[0045] The method for obtaining active polypeptide liposomes with double-layer coating by embedding treatment is specifically performed as follows: S51: Take cellulose acetate phthalate and water in a volume ratio of 1:1, heat and ultrasonically stir to obtain a solution.

[0046] S52: dispersing liposome particles in the solution, mixing and loading into a spraying device, controlling the size, speed and morphology of the piezoelectric spray head droplets according to preset parameters, and obtaining liposome particles with a coating layer.

[0047] S53: polyvinyl alcohol and water are mixed in a volume ratio of 1:1, heated and ultrasonically stirred to obtain a polyvinyl alcohol aqueous solution.

[0048] S54: dispersing the liposome particles obtained by one injection in the polyvinyl alcohol aqueous solution, mixing and loading into the injection device, and controlling the size, speed and morphology of the piezoelectric nozzle droplets according to preset parameters to obtain active polypeptide liposomes.

[0049] S6: taking active polypeptide liposomes, sodium sulfite, sucrose and tartrate in proportion, adding water and stirring to obtain an oral liquid containing a peptide composition; the oral liquid containing a peptide composition is sterilized at 100° C. and then filled and put on the market.

[0050] Example 3 This embodiment provides a peptide-containing composition for improving human immunity, which has the following components: active polypeptide liposomes, sodium sulfite, sucrose and tartrate are mixed in a ratio of 25:3:4:1 to obtain the peptide-containing composition. The active polypeptide liposomes are granular and have a double-layer embedding structure. The mass fraction of the active polypeptide liposomes is as follows: 80% polypeptide concentrate, 10% amino acids, 5% vitamins, and 5% calcium. The amino acids include glutamic acid, lysine, threonine, cystine and valine. The vitamins include vitamin A, vitamin C, vitamin D, vitamin E, vitamin B1, vitamin B2, vitamin B6 and vitamin B12.

[0051] The preparation method of the peptide-containing composition of this embodiment comprises the following steps: S1: enzymatically hydrolyze the protein to obtain a polypeptide concentrate, wherein the enzymatic hydrolysis uses a composite enzyme obtained by mixing papain, ficin and bromelain in a ratio of 1:2:3; The method for obtaining the polypeptide by enzymatic hydrolysis is specifically performed as follows: S11 takes the protein powder made from eggs and puts it into a fermentation tank; The volume ratio of S12 water to protein powder is 10:1. After adding water, the temperature is gradually raised to 40°C, and the complex enzyme is added for enzymolysis. The enzymolysis temperature is 40°C and the enzymolysis time is 60 minutes. The liquid after S13 enzymatic hydrolysis was barreled, cooled to 12°C, placed in a refrigerator for precipitation for 2 days, and then filtered to obtain a polypeptide concentrate.

[0052] S2: Take 80% of polypeptide concentrate, 10% of amino acids, 5% of vitamins and 5% of calcium in proportion by mass and mix them to obtain a mixed solution.

[0053] S3 uses supercritical CO2 method to treat soybean lecithin and mixed solution to obtain liposome solution.

[0054] The method for treating soybean lecithin and mixed liquid by supercritical CO2 method, the specific operation is as follows: S31 Take soybean lecithin and the mixed liquid in a ratio of 7:1, mix and grind.

[0055] S32 was added three times, 10 mL of 0.2 mol / L PBS phosphate buffer solution with a pH of 6.0 was added each time, and the mixture was further ground evenly, and then transferred to an autoclave.

[0056] S33: At a shear rate of 3000 r / min and a temperature of 45°C, supercritical CO2 was introduced into the autoclave until the set pressure was reached, and then ultrasound was turned on with an ultrasonic power of 18 W. The ultrasound and shearing treatments were maintained for 50 minutes. After stopping heating, shearing and ultrasound in turn, CO2 was released.

[0057] S34 added 100 mL of PBS phosphate buffer solution to rinse the particles on the inner wall of the autoclave, and treated with ultrasound and stirring for 40 minutes to obtain a liposome solution.

[0058] S4 filters the liposome solution and retains the liposome particles.

[0059] S5: embedding the liposome particles to obtain active polypeptide liposomes with double-layer coating.

[0060] The method for obtaining active polypeptide liposomes with double-layer coating by embedding treatment is specifically performed as follows: S51: Take cellulose acetate phthalate and water in a volume ratio of 1:1, heat and ultrasonically stir to obtain a solution.

[0061] S52: dispersing liposome particles in the solution, mixing and loading into a spraying device, controlling the size, speed and morphology of the piezoelectric spray head droplets according to preset parameters, and obtaining liposome particles with a coating layer.

[0062] S53: polyvinyl alcohol and water are mixed in a volume ratio of 1:1, heated and ultrasonically stirred to obtain a polyvinyl alcohol aqueous solution.

[0063] S54: dispersing the liposome particles obtained by one injection in the polyvinyl alcohol aqueous solution, mixing and loading into the injection device, and controlling the size, speed and morphology of the piezoelectric nozzle droplets according to preset parameters to obtain active polypeptide liposomes.

[0064] S6: Active polypeptide liposomes, sodium sulfite, sucrose and tartrate are taken in a ratio of 25:3:4:1, water is added and stirred to obtain an oral liquid containing a peptide composition. The oral liquid containing a peptide composition is sterilized at 100° C. and then filled and put on the market.

[0065] Example 2 is the optimal ratio of the peptide-containing composition of the present invention. Different from Example 2, the content of active polypeptide liposomes in Example 1 is the lowest, while the content of active polypeptide liposomes in Example 3 is the highest.

[0066] The above embodiments are merely preferred implementations of the present invention. Any simple modification, amendment and substitution of the above embodiments based on the technical essence of the present invention shall fall within the scope of the technical solution of the present invention.

Claims

1. A peptide-containing composition for improving human immunity, characterized in that: The peptide-containing composition comprises the following components: the active polypeptide liposome, antioxidant, sweetener and flocculant are mixed in a ratio of 8-25:1-3:2-4:1 to obtain the peptide-containing composition; The active polypeptide liposome is in granular form and has a double-layer embedding structure; the mass composition of the active polypeptide liposome is as follows: 60% to 80% of polypeptide concentrate, 10% to 40% of amino acids, 5% to 30% of vitamins, and 5% to 18% of calcium.

2. The peptide-containing composition for improving human immunity according to claim 1, characterized in that: The antioxidant is any one of sodium sulfite, sodium bisulfite, sodium pyrosulfite and vitamin C; the sweetener is any one of sucrose, stevioside, glycyrrhizin, syrup and Tiandongtian dipeptide; the flocculant is any one of citrate, hydrogen citrate, tartrate, hydrogen tartrate and phosphate.

3. The peptide-containing composition for improving human immunity according to claim 1, characterized in that: The amino acids include glutamic acid, lysine, threonine, cystine and valine.

4. The peptide-containing composition for improving human immunity according to claim 1, characterized in that: The vitamins include vitamin A, vitamin C, vitamin D, vitamin E, vitamin B1, vitamin B2, vitamin B6 and vitamin B12.

5. A method for preparing a peptide-containing composition, characterized in that: The following steps are involved: S1: protein is hydrolyzed to obtain a peptide concentrate; S2 Take the polypeptide concentrate, amino acids, vitamins and calcium according to the mass ratio, and mix them to obtain a mixed solution; S3 uses supercritical CO2 method to treat soybean lecithin and mixed solution to obtain liposome solution; S4 filters the liposome solution and retains the liposome particles; S5: performing embedding treatment on the liposome particles to obtain active polypeptide liposomes with double-layer coating; S6: taking active polypeptide liposomes, antioxidants, sweeteners and flocculants in proportion, adding water and stirring to mix, so as to obtain an oral liquid containing a peptide composition.

6. The method for preparing the peptide-containing composition according to claim 5, characterized in that: The protein source is selected from meat, eggs or milk, and the enzymatic hydrolysis uses a composite enzyme obtained by mixing papain, ficin and bromelain in a ratio of 1:2:

3.

7. The method for preparing the peptide-containing composition according to claim 6, characterized in that: The method for obtaining the polypeptide by enzymatic hydrolysis is specifically performed as follows: S11 takes protein powder made from protein and puts it into the fermentation tank. After adding water to S12, gradually heat the temperature to 40°C, and then add the complex enzyme for enzymolysis; S13 cools and filters to obtain a polypeptide concentrate.

8. The method for preparing the peptide-containing composition according to claim 7, characterized in that: The method for treating soybean lecithin and mixed liquid by supercritical CO2 method, the specific operation is as follows: S31 Take soybean lecithin and the mixed solution in a ratio of 7:1, mix and grind; S32 was added three times, 10 mL of 0.2 mol / L PBS phosphate buffer solution with a pH of 6.0 was added each time, and the mixture was further ground evenly, and then transferred to an autoclave; S33: At a shear rate of 3000 r / min and a temperature of 45°C, supercritical CO2 was introduced into the autoclave until the set pressure was reached, and then ultrasound was turned on with an ultrasound power of 18 W. The ultrasound and shearing treatment was maintained for 50 min, and the heating, shearing and ultrasound were stopped in sequence, and CO2 was released; S34 added 100 mL of PBS phosphate buffer solution to rinse the particles on the inner wall of the autoclave, and treated with ultrasound and stirring for 40 minutes to obtain a liposome solution.

9. The method for preparing the peptide-containing composition according to claim 8, characterized in that: The method for obtaining active polypeptide liposomes with double-layer coating by embedding treatment is specifically performed as follows: S51: taking an enteric material and water in a volume ratio of 1:1, heating and ultrasonically stirring to obtain a solution; S52: dispersing liposome particles in the solution, mixing and loading into a spraying device, and controlling the size, speed and morphology of the piezoelectric spray head droplets according to preset parameters to obtain liposome particles with a coating layer; S53: polyvinyl alcohol and water in a volume ratio of 1:1, heating and ultrasonically stirring to obtain a polyvinyl alcohol aqueous solution; S54: dispersing the liposome particles obtained by one injection in the polyvinyl alcohol aqueous solution, mixing and loading into the injection device, and controlling the size, speed and morphology of the piezoelectric nozzle droplets according to preset parameters to obtain active polypeptide liposomes.

10. The method for preparing the peptide-containing composition according to claim 9, characterized in that: The enteric material is any one of cellulose acetate phthalate, hydroxypropyl methylcellulose phthalate, polyvinyl phthalate and acrylic resin.