Composition for improving skin oil and pressure alopecia and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a composition for improving skin oil and pressure alopecia and application of the composition. The composition is prepared from the following components in parts by mass: 0.001 to 0.15 part of lavender essential oil and 1.0 to 5.0 parts of a wiss grass extract. Experiments prove that the composition can synergistically activate ectopic olfactory receptors in skin and hair follicles, synergistically reduce cortisol secretion and synergistically improve skin oil and pressure alopecia, so that the composition can be used in skin care cosmetics and hair cosmetics and has good practical application value.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to a composition for improving oily skin and stress-induced hair loss and an application thereof. Background Art

[0002] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention, and should not necessarily be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art.

[0003] Psychological stress can cause complex neuroendocrine reactions, mainly including increased activity of the sympathetic-adrenal medullary system (SAM) and the hypothalamus-pituitary-adrenal axis (HPA axis). Cortisol is an important stress hormone in the HPA axis, commonly known as the "stress hormone." Normally, cortisol levels are regulated by the internal biological clock system, and stress can significantly disrupt cortisol levels and oscillation curves, causing abnormal increases in cortisol, which in turn affects sebaceous gland function, skin immune function, skin barrier function, skin color, etc. Studies have shown that high levels of cortisol can increase the activity of 5α-reductase, promote the conversion of testosterone to the more potent dihydrotestosterone, increase the synthesis of skin oils, and lead to increased oil production.

[0004] In addition to the impact on the skin, long-term mental stress can also have an adverse effect on hair growth. Under normal circumstances, hair will continue to grow, fall off, and grow again, which is related to the growth cycle of hair follicles. During the growth phase, hair follicle stem cells are activated and differentiate into hair matrix cells and inner root sheath cells to produce new hair; then, the hair follicles enter the degeneration phase and the hair stops growing; then the hair follicles enter the resting phase, and after a period of time, the hair falls off. After the resting phase, the hair follicles re-enter the growth phase and new hair grows. Cortisol can directly act on hair follicle stem cells, inhibiting their proliferation and differentiation, causing the growth phase hair to enter the resting phase and fall off prematurely. An article published in Nature magazine shows that for experimental mice, when they are under stress, the adrenal glands will also release a substance similar to cortisol, namely corticosterone. When the corticosterone level of mice increases, the resting phase of many hair follicles will be prolonged, resulting in the delay in the growth of new hair. On the contrary, if the adrenal glands are removed, corticosterone can hardly be secreted, and the hair follicles in the resting phase will quickly enter the next growth phase. The active hair follicles involved in hair growth are about 3 times that of the corticosterone group. These results show that corticosterone has been at a high level for a long time, affecting the activation of hair follicle stem cells and inhibiting hair regeneration. At present, the main means of treating hair loss is the application of minoxidil, which is mainly aimed at androgenic alopecia and only plays a certain auxiliary role in the treatment of stress-induced alopecia. Skin irritation reactions may also occur during its use, such as itching, redness, swelling or burning of the scalp, and temporary aggravation of hair loss will occur within a short period of time after use. These problems have also caused the public to remain on the sidelines.

[0005] In 1991, scientists discovered that a class of GPCRs receptors can be activated by specific odor molecules, which are called olfactory receptors (ORs). To date, more than 300 functional ORs have been discovered. In addition to the nasal cavity, ORs are also widely expressed in non-olfactory tissues. ORs in non-olfactory tissues cannot distinguish odors, but can be triggered by odor molecules. They are called ectopic olfactory receptors (EORs). Studies have shown that EORs are expressed in skin tissue cells such as keratinocytes and melanocytes, and are involved in a variety of biological processes such as cell proliferation, pigment synthesis, wound healing, and hair growth.

[0006] In the prior art, patent application CN116459186A provides a soothing anti-aging essential oil composition, which can relieve emotions and has anti-aging effects, and can reduce the cortisol content in human saliva, but does not have the effect of improving oily skin and stress-induced hair loss.

[0007] Studies have shown that the ectopic olfactory receptor OR2AT4 is distributed in the epithelium of keratinocytes and hair follicles (especially the outer root sheath) and synthesizes sandalwood odorants. It can activate OR2AT4 receptors, generate calcium signals, activate downstream signaling pathways, promote the proliferation and migration of keratinocytes, promote epidermal re-epithelialization and hair growth; skin cells also contain OR1A1 receptors. After OR1A1 is activated, it can activate downstream signaling pathways through the cAMP / PKA / CREB signaling axis, in which phosphorylated CREB activates HES1, inhibits the expression of PPAR-γ, and then inhibits the expression of lipid synthesis-related genes, leading to a decrease in triglyceride, cholesterol and free fatty acid content. In the prior art, there are no research reports on regulating skin oil secretion by activating OR1A1 receptors. In the prior art, synthetic sandalwood odorants It can specifically activate OR2AT4 in hair follicles, and prolong the in vitro growth of human hair by reducing cell apoptosis and increasing the production of growth factor IGF-1, which prolongs the growth phase. Patent application CN115666728 A provides a method for preparing a sandalwood extract and the use of a composition of the consumed sandalwood extract in cosmetics for skin, scalp and appendages. The sandalwood extract can activate the ectopic olfactory receptor OR2AT4 in the skin and hair follicles, improve the epidermal barrier, brighten the skin, promote IGF-1 expression, and is active in maintaining the hair growth stage. However, it does not have the effect of improving skin oiliness and stress-induced hair loss.

[0008] Patent application CN 116899262 A provides a method for preparing a composite extract of Usma grass, which includes peach kernels, Usma grass leaves, Usma grass seeds, lavender flowers, cultivated black seed, myrtle and ginger root, and has an anti-hair loss effect. However, the patent application does not provide any proof of anti-hair loss effect, and does not have the effects of improving skin oiliness, regulating ectopic olfactory receptors and reducing cortisol. It can be seen that there are currently few cosmetic raw materials / compositions that can both regulate ectopic olfactory receptors and reduce cortisol secretion, and improve skin oiliness and stress-induced hair loss. The market urgently needs raw materials / compositions that can safely and effectively solve this problem. Summary of the invention

[0009] In view of the deficiencies in the prior art, the present invention aims to provide a composition for improving oily skin and stress-induced hair loss and its application. The composition comprises lavender essential oil and Usma grass extract. It has been verified by experiments that the composition can synergistically activate ectopic olfactory receptors in the skin and hair follicles, synergistically reduce cortisol secretion, and synergistically improve the problem of oily skin and stress-induced hair loss. Based on the above research results, the present invention is completed.

[0010] In order to achieve the above technical objectives, the technical solution provided by the present invention is as follows:

[0011] The first aspect of the present invention provides a composition for improving oily skin and stress-induced hair loss, the composition comprising the following components in parts by weight:

[0012] 0.001-0.15 parts of lavender essential oil and 1.0-5.0 parts of Usma grass extract.

[0013] In the present invention, the lavender essential oil can be obtained by commercial means or prepared according to existing techniques, and is not specifically limited here.

[0014] The Usma grass extract is prepared by extracting Usma grass. According to the "Uyghur Pharmacopoeia", Usma grass: a plant of the genus Indigofera of the Cruciferae family, a biennial herb. Contains active ingredients such as indigo glycosides, mustard glycosides, indole, indigo carmine, etc. It is cool in nature; it clears away heat and detoxifies, and removes melanin. Furthermore, the Usma grass extract is obtained by the following preparation method: mixing Usma grass with an ethanol solution, extracting by hot reflux to obtain an extract, concentrating the extract, then adding anhydrous ethanol, and refrigerating to obtain a supernatant, filtering the supernatant, concentrating it, and then adding water to let it stand, filtering it to obtain a water precipitate, and adding a preservative, water, and butanediol to the water precipitate to obtain it.

[0015] The second aspect of the present invention provides the use of the above composition in the preparation of a product for improving oily skin and stress-induced hair loss.

[0016] The product may be a cosmetic, and may further be a skin care cosmetic and / or a hair cosmetic.

[0017] The third aspect of the present invention provides a cosmetic for improving oily skin and stress-induced hair loss, wherein the cosmetic comprises the above composition; the cosmetic may be a skin care cosmetic and / or a hair cosmetic.

[0018] A fourth aspect of the present invention provides a method for preparing the above-mentioned cosmetic for improving oily skin and stress-induced hair loss, the preparation method comprising the step of mixing the composition and other raw material components.

[0019] Beneficial technical effects of one or more of the above technical solutions:

[0020] The above technical solution provides a non-addictive and safe solution to improve oily skin and stress-induced hair loss. It provides a composition composed of lavender essential oil and Usma grass extract, which: 1) can synergistically activate the mRNA expression of the ectopic olfactory receptor OR1A1 in human immortalized keratinocytes HaCaT, and alleviate the effect of stress on skin condition; 2) can synergistically activate the mRNA expression of the ectopic olfactory receptor OR2AT4 in human hair follicle keratinocytes, improve the effect of stress on hair growth, and improve stress-induced hair loss; 3) synergistically reduce cortisol secretion, thereby improving skin problems and hair loss problems caused by stress; 4) can synergistically reduce the oil content of human skin and play a role in oil control. Based on the above technical effects, the composition can synergistically improve the problems of oily skin and stress-induced hair loss.

[0021] At the same time, the cosmetic preparation method obtained by the above technical solution is simple and easy, the raw materials are easy to obtain, and it is suitable for mass production, so it has good practical application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solution of the embodiment of the present invention, the drawings required for use in the description of the embodiment will be briefly introduced below. Obviously, the drawings in the following description are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0023] Figure 1 The results of the effects of Examples 1 to 4 of the present invention and Comparative Examples 1 to 16 on the expression of ectopic olfactory receptor OR1A1 mRNA in human immortalized keratinocytes HaCaT are shown in FIG.

[0024] Figure 2 The results of the effects of Examples 1 to 4 of the present invention and Comparative Examples 1 to 16 on the expression of ectopic olfactory receptor OR2AT4 mRNA in human hair follicle keratinocytes are shown in the figure;

[0025] Figure 3 The results of the effects of Examples 1 to 4 of the present invention and Comparative Examples 1 to 2 on stress-induced hair loss in mice are shown in the figure;

[0026] in, Figure 1 and Figure 2In the table, compared with the blank control group, the significance is indicated by #, 0.01<P<0.05 is indicated by #, 0.001<P<0.01 is indicated by ##, 0.0001<P<0.001 is indicated by ###, and P<0.0001 is indicated by ####; compared with the negative control group, the significance is indicated by *, 0.01<P<0.05 is indicated by *, 0.001<P<0.01 is indicated by **, 0.0001<P<0.001 is indicated by ***, and P<0.0001 is indicated by ****. DETAILED DESCRIPTION

[0027] It should be noted that the following detailed descriptions are all illustrative and intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present invention belongs.

[0028] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0029] In a typical embodiment of the present invention, a composition for improving oily skin and stress-induced hair loss is provided, wherein the composition comprises the following components in parts by weight:

[0030] 0.001-0.15 parts of lavender essential oil and 1.0-5.0 parts of Usma grass extract.

[0031] In the present invention, the lavender essential oil can be obtained by commercial means or prepared according to existing techniques, and is not specifically limited here.

[0032] The Usma grass extract is obtained by the following preparation method: mixing Usma grass with an ethanol solution, extracting by hot reflux to obtain an extract, concentrating the extract, then adding anhydrous ethanol, refrigerating to obtain a supernatant, filtering the supernatant, concentrating it, adding water, letting it stand, filtering to obtain a water precipitate, and adding a preservative, water and butanediol to the water precipitate to obtain the extract.

[0033] In another specific embodiment of the present invention, the preparation method of the Usma grass extract comprises:

[0034] S1: Mix the Usma grass with 20% to 25% (volume fraction) ethanol solution at a solid-liquid ratio of 1:10 to 1:15 (g / mL), reflux extract at 50 to 100° C. for 0.5 h to 2 h, filter through 80 mesh to separate the Usma grass powder from the extract, and obtain a primary extract;

[0035] S2: Mix the Usma grass obtained in step S1 with 20% to 25% ethanol solution at a solid-liquid ratio of 1:5 to 1:10 (g / mL), reflux extract at 50 to 100° C. for 0.5 h to 2 h, filter, separate the Usma grass powder from the extract, and obtain a secondary extract;

[0036] S3: combining the primary extract and the secondary extract, and concentrating the mixture to 0.5-2 times (preferably 1 times) the weight of the Usma grass in step S1 to obtain a concentrated solution;

[0037] S4: adding anhydrous ethanol to the concentrated solution obtained in step S3 until the ethanol content of the system is 40-60% (preferably 50%), and placing at low temperature for more than 12 hours to obtain a supernatant;

[0038] S5: filtering the supernatant obtained in step S4 to obtain a filtrate;

[0039] S6: The filtrate obtained in step S5 is concentrated under reduced pressure to 0.1-0.5 times (preferably 0.3 times) the weight of the Usma grass in step S1, and then 0.5-1 times (preferably 0.7 times) the weight of the Usma grass is added with water, and the mixture is placed at low temperature for more than 12 hours, and then filtered to obtain a water precipitate;

[0040] S7: Add 0.1-1% (preferably 0.9%) of phenoxyethanol and 0.05-0.2% (preferably 0.1%) of ethylhexylglycerin as preservatives based on the weight of the water precipitation liquid obtained in step S6, and then add 0.1-1 times (preferably 0.4 times) of water and 0.2-0.6 times (preferably 0.6 times) of butylene glycol based on the weight of the Usma grass, mix well, and obtain the Usma grass extract.

[0041] In another specific embodiment of the present invention, in the step S1, the volume fraction of the ethanol solution is 25%, the solid-liquid ratio is 1:13, the extraction time is 1.5h, and the extraction temperature is 90-100°C; the filtration is performed by sieving, and the sieve mesh number is 60-100 mesh, preferably 80 mesh.

[0042] In the step S2, the volume fraction of the ethanol solution is 25%, the solid-liquid ratio is 1:8, the extraction time is 1 hour, and the extraction temperature is 90-100° C.; the filtration is performed by sieving, and the sieve mesh number is 60-100 mesh, preferably 80 mesh.

[0043] In the steps S4 and S6, the low temperature storage temperature may be 4°C±2°C.

[0044] In the step S5, filtration can be performed using a 0.45 μm filter plate.

[0045] In the step S6, filtration can be performed using a 0.45 μm filter plate and a 0.45 μm polypropylene membrane (PP membrane).

[0046] In another specific embodiment of the present invention, there is provided use of the above composition in the preparation of a product for improving oily skin and stress-induced hair loss.

[0047] The product may be a cosmetic. It should be noted that in the present invention, the cosmetic refers to a daily chemical industrial product that is spread on any part of the human body surface (skin, hair, nails, lips, etc.) by rubbing, spraying or other similar methods to achieve the purpose of cleaning, eliminating bad odors, skin care, beauty and modification. As can be seen from the above, the composition of the present invention has the effect of improving oily skin and stress-induced hair loss, so the cosmetic can be a skin care cosmetic and a hair cosmetic.

[0048] Therefore, in another specific embodiment of the present invention, a cosmetic for improving oily skin and stress-induced hair loss is provided, wherein the cosmetic comprises the above composition; the cosmetic may be a skin care cosmetic or a hair cosmetic.

[0049] In another specific embodiment of the present invention, in the cosmetic, the final concentration of the lavender essential oil is 0.001-0.15 wt %, and the final concentration of the Usma grass extract is 1.0-5.0 wt %.

[0050] The cosmetics may also contain any other raw material ingredients permitted to be added in the cosmetics field, including but not limited to emulsifiers, emollients, humectants and thickeners.

[0051] At the same time, the present invention can also prepare different cosmetic dosage forms by reasonably adding the above raw materials, such as liquid, gel, ointment, cream, lotion, spray, mask or freeze-dried powder, etc., which are not specifically limited here.

[0052] In another specific embodiment of the present invention, a method for preparing the above-mentioned cosmetic for improving oily skin and stress-induced hair loss is provided, and the preparation method comprises the step of mixing the composition and other raw material components.

[0053] The present invention is further described below in conjunction with the examples. The present invention is further described below by way of examples, but the present invention is not limited to the scope of the embodiments described. Based on the embodiments in the present invention, any changes to the present invention by those skilled in the art without making any creative premise belong to the protection scope of the present invention. The lavender essential oil used in the examples and comparative examples was purchased from Xinjiang Yiparhan Spice Co., Ltd.; other reagents and materials are all common commercial products unless otherwise specified.

[0054] The preparation method of the Usma grass extract described in Examples 1 to 4 is as follows:

[0055] S1: Mix the Usma grass with 25% ethanol at a solid-liquid ratio of 1:13 (g / mL), reflux extract at 90-100°C for 1.5h, filter through 80 mesh to separate the Usma grass from the extract, and obtain a primary extract;

[0056] S2: The Usma grass obtained in step S1 was mixed with 25% ethanol at a solid-liquid ratio of 1:8 (g / mL), refluxed at 90-100° C. for 1 h, filtered through 80 mesh to separate the Usma grass from the extract, and a secondary extract was obtained;

[0057] S3: combining the first extract and the second extract, and concentrating the mixture to 1 times the weight of the Usma grass to obtain a concentrated solution;

[0058] S4: Add anhydrous ethanol to the concentrated solution obtained in step S3 until the ethanol content of the system reaches 50%, refrigerate at 4°C±2°C for more than 12 hours, and retain the supernatant.

[0059] S5: Filter the supernatant obtained in step S4 using a 0.45 μm filter plate to obtain a filtrate.

[0060] S6: The filtrate obtained in step S5 is concentrated under reduced pressure to 0.3 times the weight of the Usma grass, and then 0.7 times the weight of the Usma grass is added with water, and refrigerated at 4°C±2°C for more than 12 hours, and then filtered using a 0.45 μm filter plate and a 0.45 μm PP membrane to obtain a water precipitate;

[0061] S7: Add 0.9% phenoxyethanol and 0.1% ethylhexylglycerin as preservatives to the water precipitate obtained in step S6, and then add 0.4 times the weight of water and 0.6 times the weight of butylene glycol to the water precipitate, mix well, and obtain the water precipitate extract.

[0062] In addition, in the comparative examples, unless otherwise specified, the preparation method of the added Usma grass extract is the same as that of Examples 1 to 4.

[0063] Example:

[0064] Examples 1 to 4 provide a composition that can synergistically activate ectopic olfactory receptors in the skin and hair follicles, synergistically reduce cortisol secretion, and synergistically improve skin oiliness and stress-induced hair loss problems. The composition ratio is shown in Table 1. For comparison, Table 1 also provides comparative examples 1 to 16, and the difference between comparative examples 1 to 8 and the embodiments is that only one substance in the composition is contained. The difference between comparative examples 9, 11 to 13 and the embodiments and comparative examples 2 / 6 / 7 / 8 is that only the Usma grass extract in the composition is contained, and the preparation method of the Usma grass extract is different. The difference between comparative examples 10, 14 to 16 and Example 1 is that the Usma grass extract in the composition is the Usma grass extract prepared by comparative examples 9 and 11 to 13, respectively.

[0065] in:

[0066] The preparation method of the Usma grass extract described in Comparative Examples 9 and 10 is the same as that in patent application CN116899262A.

[0067] The specific preparation process of the Usma grass extract of Comparative Examples 11 and 14 is as follows:

[0068] S1: Mix the Usma grass with 25% ethanol at a solid-liquid ratio of 1:9 (g / mL), reflux extract at 90-100°C for 1.5h, filter through 80 mesh to separate the Usma grass from the extract, and obtain a primary extract;

[0069] S2: The Usma grass obtained in step S1 was mixed with 25% ethanol at a solid-liquid ratio of 1:4 (g / mL), refluxed at 90-100° C. for 1 h, filtered through 80 mesh to separate the Usma grass from the extract, and a secondary extract was obtained;

[0070] S3: combining the first extract and the second extract, and concentrating the mixture to 1 times the weight of the Usma grass to obtain a concentrated solution;

[0071] S4: Add anhydrous ethanol to the concentrated solution obtained in step S3 until the ethanol content of the system reaches 50%, refrigerate at 4°C±2°C for more than 12 hours, and retain the supernatant.

[0072] S5: Filter the supernatant obtained in step S4 using a 0.45 μm filter plate to obtain a filtrate.

[0073] S6: The filtrate obtained in step S5 is concentrated under reduced pressure to 0.3 times the weight of the Usma grass, and then 0.7 times the weight of the Usma grass is added with water, and refrigerated at 4°C±2°C for more than 12 hours, and then filtered using a 0.45 μm filter plate and a 0.45 μm PP membrane to obtain a water precipitate;

[0074] S7: Add 0.9% phenoxyethanol and 0.1% ethylhexylglycerin as preservatives to the water precipitate obtained in step S6, and then add 0.4 times the weight of water and 0.6 times the weight of butylene glycol to the water precipitate, mix well, and obtain the water precipitate extract.

[0075] The specific preparation process of the Usma grass extract in Comparative Examples 12 and 15 is as follows:

[0076] S1: Mix the Usma grass with 75% ethanol at a solid-liquid ratio of 1:13 (g / mL), reflux extract at 90-100°C for 1.5h, filter through 80 mesh to separate the Usma grass from the extract, and obtain a primary extract;

[0077] S2: The Usma grass obtained in step S1 was mixed with 75% ethanol at a solid-liquid ratio of 1:8 (g / mL), refluxed at 90-100° C. for 1 h, filtered through 80 mesh to separate the Usma grass from the extract, and a secondary extract was obtained;

[0078] S3: combining the first extract and the second extract, and concentrating the mixture to 1 times the weight of the Usma grass to obtain a concentrated solution;

[0079] S4: Add anhydrous ethanol to the concentrated solution obtained in step S3 until the ethanol content of the system reaches 50%, refrigerate at 4°C±2°C for more than 12 hours, and retain the supernatant.

[0080] S5: Filter the supernatant obtained in step S4 using a 0.45 μm filter plate to obtain a filtrate.

[0081] S6: The filtrate obtained in step S5 is concentrated under reduced pressure to 0.3 times the weight of the Usma grass, and then 0.7 times the weight of the Usma grass is added with water, and refrigerated at 4°C±2°C for more than 12 hours, and then filtered using a 0.45 μm filter plate and a 0.45 μm PP membrane to obtain a water precipitate;

[0082] S7: Add 0.9% phenoxyethanol and 0.1% ethylhexylglycerin as preservatives to the water precipitate obtained in step S6, and then add 0.4 times the weight of water and 0.6 times the weight of butylene glycol to the water precipitate, mix well, and obtain the water precipitate extract.

[0083] The specific preparation process of the Usma grass extract in Comparative Examples 13 and 16 is as follows:

[0084] S1: Mix the Usma grass with 25% ethanol at a solid-liquid ratio of 1:13 (g / mL), reflux extract at 90-100°C for 3h, filter through 80 mesh to separate the Usma grass from the extract, and obtain a primary extract;

[0085] S2: The Usma grass obtained in step S1 was mixed with 25% ethanol at a solid-liquid ratio of 1:8 (g / mL), refluxed at 90-100° C. for 3 h, filtered through 80 mesh to separate the Usma grass from the extract, and a secondary extract was obtained;

[0086] S3: combining the first extract and the second extract, and concentrating the mixture to 1 times the weight of the Usma grass to obtain a concentrated solution;

[0087] S4: Add anhydrous ethanol to the concentrated solution obtained in step S3 until the ethanol content of the system reaches 50%, refrigerate at 4°C±2°C for more than 12 hours, and retain the supernatant.

[0088] S5: Filter the supernatant obtained in step S4 using a 0.45 μm filter plate to obtain a filtrate.

[0089] S6: The filtrate obtained in step S5 is concentrated under reduced pressure to 0.3 times the weight of the Usma grass, and then 0.7 times the weight of the Usma grass is added with water, and refrigerated at 4°C±2°C for more than 12 hours, and then filtered using a 0.45 μm filter plate and a 0.45 μm PP membrane to obtain a water precipitate;

[0090] S7: Add 0.9% phenoxyethanol and 0.1% ethylhexylglycerin as preservatives to the water precipitate obtained in step S6, and then add 0.4 times the weight of water and 0.6 times the weight of butylene glycol to the water precipitate, mix well, and obtain the water precipitate extract.

[0091] Table 1. Raw material components and proportions of the compositions of Examples 1 to 4 and Comparative Examples 1 to 16 Unit: wt%

[0092]

[0093]

[0094] A composition consisting of lavender essential oil and Usma grass extract was added to a double-layer spray matrix to prepare a skin care product with the effect of reducing skin oiliness and salivary cortisol content, namely Example 5. Comparative Examples 17 and 18 were also provided. The difference between Comparative Examples 17 and 18 and Example 5 was that Comparative Examples 17 and 18 only contained lavender essential oil or Usma grass extract. The specific raw material components and proportions are shown in Table 2. The effects of improving skin oiliness and reducing salivary cortisol content were tested through human efficacy experiments.

[0095] Table 2. Double-layer spray raw material ingredients and ratios for Example 5 and Comparative Examples 17 and 18

[0096]

[0097] The products of Example 5, Comparative Example 17 and Comparative Example 18 are all prepared by conventional methods in the art and need to be shaken before use to mix them.

[0098] A composition consisting of lavender essential oil and Usma grass extract was added to a shampoo base to prepare a shampoo with the effect of improving stress-induced hair loss, i.e., Example 6. At the same time, a placebo group, i.e., Comparative Example 19, was set up. The difference between Comparative Example 19 and Example 6 was that Comparative Example 19 did not contain a composition consisting of lavender essential oil and Usma grass extract. The specific raw material components and proportions are shown in Table 3.

[0099] Table 3. Raw material ingredients and proportions of shampoo in Example 6 and Comparative Example 19

[0100]

[0101]

[0102] The products of Example 6 and Comparative Example 19 were prepared by conventional methods in the art.

[0103] Experimental example:

[0104] The performance tests of the products of the above embodiments and comparative examples are as follows:

[0105] Experimental material preparation:

[0106] The mouse data used in the following tests are from Qilu University of Technology; human hair follicle keratinocytes are from Shanghai Jingkang Bioengineering Co., Ltd.; human immortalized keratinocytes HaCaT are from the Shanghai Cell Bank of the Chinese Academy of Sciences; other reagents and materials are common commercial products unless otherwise specified.

[0107] 1. Effects of Examples 1 to 4 and Comparative Examples 1 to 16 on the mRNA expression of ectopic olfactory receptor OR1A1 in keratinocytes HaCaT

[0108] 1. Experimental method: Collect human immortalized keratinocytes HaCaT in good growth state and prepare cell suspension with complete culture medium. Adjust the cell concentration to 1.3×10 5 / mL, add 2mL / well cell suspension to a 6-well cell culture plate. The experiment sets up a blank control group (high-glucose DMEM culture fluid), a negative control group (10nM cortisol + high-glucose DMEM culture fluid), and a sample group (10nM cortisol + high-glucose DMEM culture fluid containing Examples 1 to 4 or Comparative Examples 1 to 16), and each group is set up 3 parallels. Place in a cell culture incubator (5% CO2, 37°C) and culture for 24h, the cell fusion rate reaches 40-60%, and the complete culture fluid is discarded. The blank control group is added with high-glucose DMEM culture fluid, and the remaining groups are all added with high-glucose DMEM culture fluid containing 10nM cortisol and cultured in a cell culture incubator for 12h, then the liquid in the well plate is discarded, PBS buffer is added and washed twice, and high-glucose DMEM culture fluid is added to the blank control group and the negative control group, and the sample group is added with high-glucose DMEM culture fluid containing Examples 1 to 4 or Comparative Examples 1 to 16 and cultured in a cell culture incubator for 12h. The culture medium was discarded, the cells were collected, RNA was extracted, and the relative expression level of ectopic olfactory receptor OR1A1 mRNA was detected by RT-qPCR.

[0109] The test concentrations of the composition samples of Examples 1 to 4 and Comparative Examples 1 to 16 are shown in Table 1, and the solvent is a high-glucose DMEM culture medium containing 0.1% (v / v) DMSO. The synergistic effect of the composition was determined using King's formula:

[0110] Q = E(a+b) / (Ea+Eb-Ea×Eb);

[0111] Among them, Q<0.55 is obvious antagonism, Q=0.55-0.85 is antagonism, Q=0.85-1.15 is simple addition, and Q>1.15 is synergistic.

[0112] 2. Experimental results: Figure 1As shown in Table 4, after cortisol stimulation, the relative expression of ectopic olfactory receptor OR1A1 mRNA in the negative control group was significantly lower than that in the blank control group (P < 0.001), indicating that stress can affect the expression of ectopic olfactory receptors in skin keratinocytes; compared with the negative control group, after cortisol stimulation, after adding the compositions of Examples 1 to 4 and Comparative Examples 1 to 16 to act on the keratinocyte stress model, except for Comparative Examples 2, 6, 7, 8, 9, 11, 12, and 13, the relative expression of ectopic olfactory receptor OR1A1 mRNA was significantly increased (P < 0.05). Among them, the improvement rates of the relative expression levels of the ectopic olfactory receptor OR1A1 mRNA in Examples 1 to 4 were 113.62%, 103.96%, 110.34%, and 60.53%, respectively, and the improvement rates of the relative expression levels of the ectopic olfactory receptor OR1A1 mRNA in Comparative Examples 1 to 16 were 38.99%, -0.40%, 43.46%, 51.75%, 32.32%, -1.17%, 3.17%, 9.45%, 1.01%, 40.88%, -5.95%, -7.87%, -1.49%, 36.68%, 32.33%, and 38.95%, respectively. The analysis shows that the use of lavender essential oil alone in comparative examples 1, 3, 4, and 5 has a certain effect on increasing the relative expression of the ectopic olfactory receptor OR1A1 mRNA in keratinocytes. The use of Usma grass extract alone in comparative examples 2, 6, 7, and 8 shows that the relative expression of the ectopic olfactory receptor OR1A1 mRNA in keratinocytes is inhibited at low concentrations and slightly increased at high concentrations, but there is no significant difference. The use of Usma grass extract prepared by a different process from the example alone in comparative examples 9, 11, 12, and 13 has no effect on increasing the relative expression of the ectopic olfactory receptor OR1A1 mRNA in keratinocytes. Compared with the results of Examples 1 to 4, it can be seen that the improvement effect is significantly increased when the two are used in combination; taking Example 1 as an example, E(a+b)=113.62%, Ea=38.99%, Eb=-0.40%, and substituting into King's formula to obtain Q=2.93>1.15, it shows synergistic effect; similarly, the lavender essential oil and Usma grass compositions of Examples 2 to 4 both achieve synergistic effect in increasing the relative expression of ectopic olfactory receptor OR1A1 mRNA in keratinocytes. Comparative Example 10 has E(a+b)=40.88%, Ea=38.99%, Eb=1.01%, and substituting into King's formula to obtain Q=1.03<1.15, which is a simple addition of the effects of the two substances, and there is no synergistic effect. Similarly, the Q of Comparative Example 14 is 1.04<1.15, the Q of Comparative Example 15 is 0.94<1.15, and the Q of Comparative Example 16 is 1.02<1.15, which are all the effects of the two substances added together, and there is no synergistic effect. The above results show that the extract of Usma grass and lavender essential oil under the processes of Examples 1 to 4 have a synergistic effect.

[0113] Table 4. Effects of Examples 1 to 4 and Comparative Examples 1 to 16 on the mRNA expression of ectopic olfactory receptor OR1A1 in keratinocytes HaCaT

[0114]

[0115]

[0116] II. Effects of Examples 1 to 4 and Comparative Examples 1 to 16 on the mRNA Expression of Ectopic Olfactory Receptor OR2AT4 in Human Hair Follicle Keratinocytes

[0117] 1. Experimental method: Human hair follicle keratinocytes with good growth status were collected and cell suspension was prepared with EliteCell primary keratinocyte complete culture medium. The cell concentration was adjusted to 1.0×10 5 / mL, add 2mL / well cell suspension to a 6-well cell culture plate. The experiment set up a blank control group (EliteCell primary keratinocyte culture fluid), a negative control group (10nM cortisol + EliteCell primary keratinocyte culture fluid), and a sample group (10nM cortisol + EliteCell primary keratinocyte culture fluid containing Examples 1 to 4 or Comparative Examples 1 to 16), and each group was set up with 3 parallels. Place in a cell culture incubator (5% CO2, 37°C) and culture for 24h. When the cell fusion rate reaches 40-60%, discard the EliteCell primary keratinocyte complete culture fluid. The blank control group was added with EliteCell primary keratinocyte culture medium, and the other groups were added with EliteCell primary keratinocyte culture medium containing 10nM cortisol and cultured in a cell culture incubator for 12h, then the liquid in the well plate was discarded, and PBS buffer was added to wash twice, the blank control group and the negative control group were added with EliteCell primary keratinocyte culture medium, and the sample group was added with EliteCell primary keratinocyte culture medium containing Examples 1 to 4 or Comparative Examples 1 to 16 and cultured in a cell culture incubator for 12h. The culture medium was discarded, the cells were collected, RNA was extracted, and the relative expression of the ectopic olfactory receptor OR2AT4 mRNA was detected by RT-qPCR.

[0118] The test concentrations of Examples 1 to 4 and Comparative Examples 1 to 16 are shown in Table 1, and the solvent is EliteCell primary keratinocyte culture medium containing 0.1% (v / v) DMSO. The synergistic effect of the composition was determined using King's formula:

[0119] Q = E(a+b) / (Ea+Eb-Ea×Eb);

[0120] Among them, Q<0.55 is obvious antagonism, Q=0.55-0.85 is antagonism, Q=0.85-1.15 is simple addition, and Q>1.15 is synergistic.

[0121] 2. Experimental results: Figure 2 As shown in Table 5, after cortisol stimulation, the relative expression of ectopic olfactory receptor OR2AT4 mRNA in human hair follicle keratinocytes in the negative control group was significantly lower than that in the blank control group (P < 0.01), indicating that stress can affect the expression of ectopic olfactory receptors in human hair follicle keratinocytes; compared with the negative control group, after cortisol stimulation, the relative expression of ectopic olfactory receptor OR2AT4 mRNA was significantly increased after adding the compositions of Examples 1 to 4 and Comparative Examples 1 to 16 to the human hair follicle keratinocyte stress model (P < 0.05). Among them, the relative expression rates of the ectopic olfactory receptor OR2AT4 mRNA in Examples 1 to 4 were 113.34%, 96.06%, 105.44%, and 70.64%, respectively, and the relative expression rates of the ectopic olfactory receptor OR2AT4 mRNA in Comparative Examples 1 to 16 were 33.75%, 25.53%, 47.59%, 66.47%, 26.95%, 20.08%, 35.11%, 44.04%, 18.47%, 45.39%, 18.72%, 17.91%, 23.50%, 49.22%, 40.03%, and 52.36%, respectively. Analysis shows that the use of lavender essential oil or Usma grass alone has a certain effect on increasing the relative expression of the ectopic olfactory receptor OR2AT4 mRNA in human hair follicle keratinocytes. However, compared with the results of Examples 1 to 4, it can be seen that the lifting effect is significantly increased when the two are used in combination; taking Example 1 as an example, E(a+b)=113.34%, Ea=33.75%, Eb=25.53%, and substituting into King's formula to obtain Q=2.24>1.15, it is shown as synergistic enhancement; similarly, the lavender essential oil and Usma grass compositions of Examples 2 to 4 both achieve synergistic enhancement in terms of increasing the relative expression of ectopic olfactory receptor OR2AT4 mRNA in human hair follicle keratinocytes, and can improve the effect of pressure on hair growth. Comparative Example 10 has E(a+b)=45.39%, Ea=33.75%, Eb=18.47%, and substituting into King's formula to obtain Q=0.99<1.15, which is a simple addition of the effects of the two substances, and there is no synergistic effect. Similarly, the Q of Comparative Example 14 is 1.07<1.15, the Q of Comparative Example 15 is 0.88<1.15, and the Q of Comparative Example 16 is 1.06<1.15, which are all the effects of the two substances added together, and there is no synergistic effect. The above results show that the extract of Usma grass under the processes of Examples 1 to 4 has a synergistic effect with lavender essential oil.

[0122] Table 5. Effects of Examples 1 to 4 and Comparative Examples 1 to 16 on the expression of OR2AT4 in human hair follicle keratinocytes

[0123]

[0124]

[0125] III. Effects of Examples 1 to 4 and Comparative Examples 1 to 2 on Stress-induced Hair Loss in Mice

[0126] 1. Experimental method: Preparation of corticosterone solution: Dissolve in physiological saline containing 0.1% DMSO and 0.1% Tween-80, with a final concentration of 4 mg / mL of corticosterone;

[0127] Solutions of Examples 1 to 4 and Comparative Examples 1 to 2: Dissolved in physiological saline containing 0.1% DMSO, the final concentrations of the compositions of Examples 1 to 4 and Comparative Examples 1 to 2 are shown in Table 1;

[0128] Mice were raised in the animal room of Qilu University of Technology. Female mice were used, weighing 18-20 g. The experiment set up a blank control group (injection of physiological saline containing 0.1% DMSO and 0.1% Tween-80 + application of physiological saline containing 0.1% DMSO), a negative control group (injection of corticosterone solution + application of physiological saline containing 0.1% DMSO) and a sample group (injection of corticosterone solution + application of Example 1-4 / Comparative Example 1-2 solution), 6 mice in each group, 12h of light environment-12h of dark environment, alternating. The temperature was maintained at 22°C ± 1°C, and the humidity was 30% to 70%.

[0129] After 3 days of adaptive feeding, the hair on the back of the mice was shaved short using a small animal shaver, and a depilatory cream was applied to remove the remaining hair. When the mouse skin was pink and undamaged, it was confirmed that its hair follicles were in the resting phase. The negative control group and the sample group were subcutaneously injected with a 20 mg / kg dose of corticosterone solution in the depilatory area of ​​the back of the mice at multiple points, once a day, and the blank control group was injected with an equal volume of saline containing 0.1% DMSO and 0.1% Tween-80, and the injection was continued for 3 weeks to model. The sample group began to apply the solutions of Examples 1 to 4 and Comparative Examples 1 to 2 one week after modeling, once a day, 50 μL each time, for 4 consecutive weeks, and the blank control group and the negative control group were applied with an equal volume of saline containing 0.1% DMSO, and photographed and compared the hair growth in the 4th week.

[0130] 2. Experimental results: Four weeks after applying the sample, the hair loss areas on the backs of mice in each group were photographed and observed. Figure 3As shown, the back hair of the mice in the blank control group has completely recovered without any exposed skin, the back hair removal site of the mice in the negative control group still has a large amount of exposed skin, and the back hair of the mice in Examples 1 to 4 and Comparative Examples 1 to 2 has grown to varying degrees. From the analysis of the results of the apparent growth of hair, both Comparative Examples 1 and 2 have a certain effect of promoting hair growth, and the effect of promoting hair growth in Examples 1 to 4 is better than that in Comparative Examples 1 to 2, and no adverse reactions or recurrence after discontinuation of use occur, showing a synergistic effect.

[0131] IV. Effects of Example 5 and Comparative Examples 17 and 18 on the Oil Content of Human Skin

[0132] 1. Selection and requirements of volunteers

[0133] 90 male volunteers were selected. They reported that they were under high stress for a long time. All of them had oily skin and the oil content on their forehead was ≥120μg / cm 2 , aged 18 to 35 years old, were divided into three groups (Example 5 group, Comparative Example 17 group and Comparative Example 18 group), each group had 30 people, all subjects had no history of skin or systemic diseases, no abnormalities in the tested parts, and did not apply any drugs or cosmetics unrelated to the experiment during the test.

[0134] 2. Test environment

[0135] The test site is kept at a constant temperature and humidity, with an ambient temperature of 20℃ to 22℃ and a relative humidity of 40% to 60%. The subjects should keep their bodies in a stable state before the test. After washing the face of the subject with clean water at about 35℃, the subject should sit quietly for 30 minutes in the test environment before starting the test.

[0136] 3. Sample usage

[0137] The test samples are the product of Example 5, the product of Comparative Example 17 and the product of Comparative Example 18. The bases of Comparative Examples 17 and 18 are the same as those of Example 5, except that only one of lavender essential oil or Usma grass extract is contained in Comparative Examples 17 and 18. Use twice a day, shake the product well after cleansing in the morning and evening, and use it once each for 28 consecutive days.

[0138] 4. Test Project

[0139] (1) Experimental method: The skin oil content tester Sebumeter (SM815, Courage and Khazaka, Germany) was used to test the skin oil content on the 0th day and the 28th day after using the product. The skin oil content tester uses the world-recognized SEBUMETER method to characterize the skin oil content by measuring the change in light transmittance of a 0.1 mm thick special matte tape before and after absorbing oil. During the test, the tape is in contact with the test area and becomes translucent after absorbing the oil on the human skin. The light transmittance also changes accordingly. The more oil absorbed, the greater the light transmittance. Measuring the light transmittance of the tape can measure the skin oil content.

[0140] Cortisol content test: A high-sensitivity enzyme immunoassay kit (ELISA) was used to test the cortisol content in the saliva of 90 volunteers on the 0th and 28th days of using the product at the same time point. The volunteers rinsed their mouths 30 minutes before the test and did not eat or drink for 30 minutes. Then, the tampon in the saliva collection tube was held under the tongue for about 5 minutes. After the tampon was completely soaked, it was put back into the tube and centrifuged at 3000r / min for 15 minutes to collect the liquid for cortisol content detection.

[0141] The synergistic effect of the combination was determined using King's formula:

[0142] Q = E(a+b) / (Ea+Eb-Ea×Eb);

[0143] Among them, Q<0.55 is obvious antagonism, Q=0.55-0.85 is antagonism, Q=0.85-1.15 is simple addition, and Q>1.15 is synergistic.

[0144] (2) Experimental results: As shown in Table 6, after the volunteers used the test samples for 28 days, the oil content of the Example 5 group decreased by 29.49%, the oil content of the Comparative Example 17 group decreased by 2.21%, and the oil content of the Comparative Example 18 group decreased by 4.02%, and no adverse reactions occurred; according to King's formula, E(a+b)=29.49%, Ea=2.21%, Eb=4.02%, and substituting into King's formula, Q=4.80>1.15 is obtained, which proves that the skin care product containing the composition composed of lavender essential oil and Usma grass extract can synergistically reduce the oil content of the skin , which plays an oil-control role; after the volunteers used the test samples for 28 days, the cortisol content in the saliva of the Example 5 group decreased by 19.32%, the cortisol content in the saliva of the comparative example 17 group decreased by 2.21%, and the cortisol content in the saliva of the comparative example 18 group decreased by 5.04%; according to the King's formula, E(a+b)=19.32%, Ea=2.21%, Eb=5.04%, and Q=2.71>1.15 was obtained by substituting into the King's formula, which proves that the skin care product containing the composition composed of lavender essential oil and Usma grass extract can synergistically reduce the cortisol content in saliva and reduce stress.

[0145] Table 6. Effects of Example 5, Comparative Example 17 and Comparative Example 18 on the oil content of human skin and the cortisol content in saliva

[0146]

[0147] Note: Compared with Comparative Example 17 and Comparative Example 18, P < 0.05 is considered to be significantly different.

[0148] The above results indicate that the composition comprising lavender essential oil and Usma grass extract can synergistically activate ectopic olfactory receptors in the skin and hair follicles, synergistically reduce the secretion of cortisol, and synergistically improve the problems of oily skin and stress-induced hair loss.

[0149] It should be noted that the above examples are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention is described in detail with reference to the given examples, those skilled in the art may modify or replace the technical solution of the present invention as needed without departing from the spirit and scope of the technical solution of the present invention.

Claims

1. A composition for improving oily skin and stress-induced hair loss, characterized in that: The composition comprises the following components in parts by weight: 0.001-0.15 parts of lavender essential oil, 1.0-5.0 parts of Usma grass extract; The Usma grass extract is obtained by the following preparation method: mixing Usma grass with an ethanol solution, extracting by hot reflux to obtain an extract, concentrating the extract, then adding anhydrous ethanol, refrigerating to obtain a supernatant, filtering the supernatant, concentrating it, adding water, letting it stand, filtering to obtain a water precipitate, and adding a preservative, water and butanediol to the water precipitate to obtain the extract.

2. The composition according to claim 1, characterized in that The preparation method of the Usma grass extract comprises: S1: Mix the Usma grass with 20% to 25% (volume fraction) ethanol solution at a solid-liquid ratio of 1:10 to 1:15 (g / mL), reflux extract at 50 to 100° C. for 0.5 h to 2 h, filter through 80 mesh to separate the Usma grass powder from the extract, and obtain a primary extract; S2: Mix the Usma grass obtained in step S1 with 20% to 25% ethanol solution at a solid-liquid ratio of 1:5 to 1:10 (g / mL), reflux extract at 50 to 100° C. for 0.5 h to 2 h, filter, separate the Usma grass powder from the extract, and obtain a secondary extract; S3: combining the primary extract and the secondary extract, and concentrating the mixture to 0.5-2 times the weight of the Usma grass in step S1 to obtain a concentrated solution; S4: adding anhydrous ethanol to the concentrated solution obtained in step S3 until the ethanol content of the system is 40-60%, and placing at low temperature for more than 12 hours to obtain a supernatant; S5: filtering the supernatant obtained in step S4 to obtain a filtrate; S6: The filtrate obtained in step S5 is concentrated under reduced pressure to 0.1-0.5 times the weight of the Usma grass in step S1, and then 0.5-1 times the weight of the Usma grass is added with water, and the mixture is placed at low temperature for more than 12 hours, and then filtered to obtain a water precipitate; S7: Add 0.1-1% phenoxyethanol and 0.05-0.2% ethylhexylglycerin as preservatives to the water precipitate obtained in step S6, then add 0.1-1 times of water and 0.2-0.6 times of butylene glycol by weight of the Usma grass, mix well, and obtain the Usma grass extract.

3. The composition according to claim 2, characterized in that In the step S1, the volume fraction of the ethanol solution is 25%, the solid-liquid ratio is 1:13, the extraction time is 1.5 hours, and the extraction temperature is 90-100° C.; the filtration is performed by sieving, and the sieve mesh number is 60-100 mesh, preferably 80 mesh.

4. The composition according to claim 2, characterized in that In the step S2, the volume fraction of the ethanol solution is 25%, the solid-liquid ratio is 1:8, the extraction time is 1 hour, and the extraction temperature is 90-100° C.; the filtration is performed by sieving, and the sieve mesh number is 60-100 mesh, preferably 80 mesh.

5. The composition according to claim 2, characterized in that In the steps S4 and S6, the low temperature storage temperature is 4°C±2°C.

6. The composition according to claim 2, characterized in that In the step S5, filtering is performed using a 0.45 μm filter plate; In the step S6, filtration is performed using a 0.45 μm filter plate and a 0.45 μm polypropylene membrane.

7. Use of the composition according to any one of claims 1 to 6 in the preparation of a product for improving oily skin and stress-induced hair loss.

8. The use according to claim 7, characterized in that The product is a cosmetic; further, a skin care cosmetic and / or a hair cosmetic.

9. A cosmetic for improving oily skin and stress-induced hair loss, characterized in that: The cosmetic comprises the composition according to any one of claims 1 to 6; further, the cosmetic is a skin care cosmetic and / or a hair cosmetic.

10. The method for preparing the cosmetic for improving oily skin and stress-induced hair loss according to claim 9, characterized in that: The preparation method comprises the step of mixing the composition and other raw material components.

Images