Glycyrrhiza inflata anti-allergy soothing lotion and application thereof in cosmetics

Abstract

The invention discloses glycyrrhiza inflate anti-allergy soothing lotion and application of the glycyrrhiza inflate anti-allergy soothing lotion in cosmetics. The glycyrrhiza inflate anti-allergy soothing lotion comprises the following components: a rhodiola rosea root extract, a panax notoginseng root extract and a glycyrrhiza inflate root extract. According to the extraction process, degreasing and enzymolysis methods are adopted, the two processes supplement each other, and compared with a single extraction method, the extraction rate of active substances such as polysaccharide is higher; tests prove that the prepared anti-allergy soothing lotion can effectively relieve the allergy problems such as redness, dry itching, scorching hot and stabbing pain, and can be added into a cosmetic matrix as an active component to achieve a good anti-allergy repairing effect.

Description

Technical Field

[0001] The invention relates to the field of cosmetics, and in particular to an anti-allergic soothing liquid of Glycyrrhiza inflata and application thereof in cosmetics. Background Art

[0002] In modern society, with environmental pollution, lifestyle changes and the widespread use of cosmetics, the problem of sensitive skin has become increasingly prominent. Sensitive skin, in medicine, usually refers to the skin condition in which the skin overreacts to external stimuli such as temperature changes, chemicals, ultraviolet rays, etc., and symptoms such as redness, swelling, stinging, and itching appear. There are many ways to treat sensitive skin, among which drug therapy and cosmetic therapy are the two main ways.

[0003] Oral medication is one of the important means of treating sensitive skin. Antihistamines are commonly used oral antiallergic drugs, which can block histamine receptors, reduce vasodilation and increased permeability caused by allergic reactions, and thus relieve symptoms such as redness, swelling, and itching. However, clinical studies have found that some drugs have potential effects on the gastrointestinal tract and liver and kidney function. Topical medications act directly on the surface of the skin and can quickly relieve sensitive symptoms. Glucocorticoid ointments are common topical anti-inflammatory drugs that relieve skin symptoms by suppressing immune and inflammatory responses. However, long-term use of glucocorticoids can lead to side effects such as thinning of the skin and pigmentation. [1] .

[0004] In recent years, studies have found that polysaccharides have many pharmacological effects, such as anti-tumor, anti-virus, anti-aging, anti-hypoxia, anti-damage, antioxidant activity, anti-inflammatory, and immune enhancement. In addition, the large number of hydrophilic hydroxyl groups linked to polysaccharides make them highly absorbent, emulsifiable, highly viscous, and film-forming. The pharmacological activity and physicochemical properties of polysaccharides provide a basis for their application in cosmetics. When used in cosmetics, they can produce moisturizing, repair damaged skin, anti-inflammatory, anti-aging, and antioxidant effects. [2] .

[0005] The active ingredients added to skin care products are not as powerful, long-lasting, and do not directly change the structure and function of the skin as drugs, but skin care products focus on daily care and maintenance of the skin. By adding active ingredients such as polysaccharides developed from natural plants with anti-allergic, anti-inflammatory, and antipruritic effects, combined with the moisturizing and nutritional effects of skin care products themselves, they have a good effect in maintaining and repairing sensitive skin. Skin care products have few toxic side effects and are not addictive, which has been warmly welcomed by consumers. Skin care products with anti-allergic and soothing effects have become a hot spot in the industry's research and development.

[0006] References [1]: Yang Libo, Qiu Shi, He Li, et al. Application of drug and non-drug therapies in the treatment of sensitive skin [J]. Health Must-Read, 2024, (11): 75.

[0007] References [2]: Zhang Bin, Zhang Lu, Li Shasha, et al. The relationship between plant polysaccharides and cosmetics [J]. Journal of Liaoning University of Traditional Chinese Medicine, 2013, 15(01): 109-111. Summary of the invention

[0008] The technical problem to be solved by the present invention is to provide a licorice anti-allergic soothing liquid for sensitive skin.

[0009] The specific technical solutions of the present invention are as follows: A glycyrrhiza inflata anti-allergic soothing solution comprises the following components: rhodiola rosea root extract, PANAX notoginseng root extract and glycyrrhiza inflata root extract.

[0010] The embodiment of the present invention discloses a preparation process of the Glycyrrhiza inflata anti-allergic soothing solution, and the specific steps are as follows: (1) Weigh the following raw materials in parts by weight: 1-10 parts of Rhodiola rosea root, 1-10 parts of Panax notoginseng root, and 1-10 parts of Glycyrrhiza inflata root; dry and crush to obtain a composite powder; perform a defatting treatment to obtain a defatted product; (2) adding 5-10 times the weight of water to the above-mentioned defatted product, and adding enzymes for enzymolysis to obtain an enzymolysis product; (3) Raise the temperature to 95-100°C, keep warm and stir for 1-2 hours; then centrifuge at 3000-5000 rpm for 10-30 minutes and take the supernatant; (4) Concentrate the supernatant by rotary evaporation to 1 / 10-1 / 20 of the original volume, then add 4-5 times the volume of anhydrous ethanol, let it stand at 1-5°C for 12-24 hours, centrifuge at 3000-5000 rpm for 10-30 minutes, collect the precipitate; then add deionized water 10-20 times the weight of the precipitate, stir and dissolve, and obtain the Glycyrrhiza uralensis anti-allergic soothing solution.

[0011] Preferably, the drying refers to using a drying method commonly used in the art, such as heat drying or freeze drying, so that the water content of the raw materials is less than 1.5%, more preferably, less than 0.5%.

[0012] In some embodiments, the raw materials are as follows: 5 parts by weight of Rhodiola rosea root, 3 parts by weight of Panax notoginseng root, and 8 parts by weight of Glycyrrhiza inflata root.

[0013] Preferably, the degreasing treatment is intended to help disintegrate the cell membrane, avoid the inhibition of subsequent enzymatic hydrolysis by lipids such as fatty acids, and help the extraction rate of highly polar active substances such as polysaccharides. Specifically, degreasing agents acceptable in the cosmetics field can be used, such as anhydrous ethanol, petroleum ether, etc. In some of the embodiments, the degreasing process used is: weigh 5-10 times the weight of the composite powder with anhydrous ethanol, slowly stir at room temperature for 10-20 hours, then centrifuge to obtain a precipitate, and then rotary evaporate to dry, remove the ethanol, and obtain a defatted product.

[0014] Preferably, the enzymatic hydrolysis is intended to help the plant material degrade to form a more sparse structure, so that the effective ingredients are easier to release. In some embodiments, the enzymatic hydrolysis uses a mixture of neutral cellulase and protease, which acts on different components of the plant, such as cell walls and proteins, which can effectively reduce viscosity and increase filtration speed. The enzymatic hydrolysis process used is: adjust the pH value to 7-8, the temperature to 42-46°C, and act for 4-8 hours under stirring; wherein the amount of neutral cellulase added is 0.1-1% of the dry weight of the defatted product, and the amount of protease added is 0.1-1% of the dry weight of the defatted product.

[0015] The anti-allergic soothing liquid of Glycyrrhiza uralensis prepared in the embodiment of the present invention can be used as an active ingredient in cosmetic formulas. Preferably, the amount of the anti-allergic soothing liquid of Glycyrrhiza uralensis added to the cosmetic matrix formula is 1-10%; the cosmetic matrix can be skin care and hair care products such as creams, lotions, facial masks, essences, water solutions, facial cleansers, conditioners, shampoos, etc.

[0016] The embodiment of the present invention also discloses a sensitive skin soothing cream, comprising the following components in percentage by weight: 2% glycerin, 0.25% ammonium acryloyldimethyltaurate / VP copolymer, 0.5% acrylic acid (esters) / C10-30 alkyl acrylate cross-linked polymer, 0.1% xanthan gum, 0.05% sodium hyaluronate, 0.25% allantoin, 0.5% p-hydroxyacetophenone, 0.35% PEG-20 methyl glucose sesquistearate, 0.5% tocopheryl acetate, 1.7% polydimethylsiloxane, 2% PEG / PPG-17 / 6 copolymer, 0.7% 1,2-hexanediol, 0.5% triethanolamine, 4% of the above-mentioned Glycyrrhiza inflata anti-allergic soothing solution, and the balance water.

[0017] The beneficial effects brought about by the technical solution provided by the embodiment of the present invention are as follows: the present invention selects traditional Chinese characteristic plants such as Rhodiola rosea root, Panax notoginseng root, and Glycyrrhiza uralensis root, and its extraction process adopts defatting and enzymatic hydrolysis methods. The two processes complement each other. Compared with a single extraction method, the extraction rate of active substances such as polysaccharides is higher; and the solvents used in each extraction process are all substances acceptable in the cosmetics field, and no new pollutants / impurities will be introduced due to the process; tests have proved that the prepared anti-allergic soothing solution can effectively relieve allergic problems such as redness, dry itching, burning, and stinging, and can be added to the cosmetic matrix as an active ingredient to play a good anti-allergic repair role. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for describing the embodiments or the prior art are briefly introduced below.

[0019] Figure 1 Shows the polysaccharide and total sugar content of each test group in Experiment 1. DETAILED DESCRIPTION

[0020] The embodiments in the specification are only used to illustrate the present invention and do not limit the scope of protection of the present invention. The scope of protection of the present invention is only limited by the claims, and any omission, replacement or modification made on the basis of the embodiments disclosed in the present invention shall fall within the scope of protection of the present invention.

[0021] The content of the present invention can be more easily understood by selecting the following detailed description of the preferred implementation method of the present invention and the embodiments included. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those of ordinary skill in the art to which the present invention belongs. When there is a conflict, the definition in this specification shall prevail.

[0022] As used herein, the term "prepared from" is synonymous with "comprising." As used herein, the terms "comprising," "including," "having," "containing," or any other variation thereof, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises the listed elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

[0023] When amount, concentration or other value or parameter is expressed as range, preferred range or a series of upper preferred value and lower preferred value limit range, this should be understood as specifically disclosing all ranges formed by any pairing of any range upper limit or preferred value and any range lower limit or preferred value, regardless of whether the range is disclosed separately. For example, when disclosing range "1 to 5", described range should be interpreted as including range "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5" etc. When numerical range is described in this article, unless otherwise stated, the range is intended to include its end value and all integers and fractions within the range.

[0024] In addition, the indefinite articles "a" and "an" before the elements or components of the present invention have no restrictions on the quantity requirements (i.e. the number of occurrences) of the elements or components. Therefore, "a" or "an" should be interpreted as including one or at least one, and the elements or components in the singular form also include the plural form, unless the number is obviously intended to be in the singular form.

[0025] In order to deepen the understanding of the present invention, the present invention will be further described in detail below in conjunction with examples. The examples are only used to explain the present invention and do not constitute a limitation on the protection scope of the present invention.

[0026] The Rhodiola rosea described in the present invention is a perennial herbaceous plant of the genus Rhodiola of the Crassulaceae family. Nature and flavor: cold, sweet, astringent. Meridian: Lung meridian. Functions and indications: It has the effects of invigorating qi and clearing the lungs, improving intelligence and nourishing the heart, astringing and stopping bleeding, and dispersing blood stasis and reducing swelling. It is mainly used to treat qi deficiency and physical weakness, fear of cold after illness, shortness of breath and fatigue, cough due to lung heat, hemoptysis, leucorrhea and diarrhea, and traumatic injuries. Rhodiola rosea grows in harsh environments, such as high altitude, severe cold, strong ultraviolet rays, etc., and has strong stress resistance. It is reported that Rhodiola rosea is rich in a variety of bioactive substances, and polysaccharides are one of the main bioactive components of Rhodiola rosea, which is a natural macromolecule. Rhodiola rosea polysaccharide has a strong antioxidant capacity, can scavenge free radicals, inhibit lipid peroxidation, and protect cells from damage. At the same time, it can also promote the body's metabolic process and accelerate the repair of damaged tissues.

[0027] The Panax notoginseng described in the present invention is a perennial herbaceous plant of the genus Panax of the Araliaceae family. Zhao Xuemin of the Qing Dynasty recorded in "Supplement to Compendium of Materia Medica": Ginseng is the best for replenishing qi, and Panax notoginseng is the best for replenishing blood. They have the same taste and the same function, so they are called ginseng Panax notoginseng, which is the most precious among Chinese medicines. Panax notoginseng is warm in nature, sweet and slightly bitter. It enters the liver and stomach meridians. It has the effects of replenishing blood and anti-inflammation, promoting blood circulation and removing blood stasis, and can resist oxidation and anti-aging. Panax notoginseng can dilate blood vessels, reduce coronary resistance, increase coronary flow, strengthen and improve coronary microcirculation, increase nutritional myocardial blood flow, and can also reduce arterial pressure and slightly reduce heart rate, thereby significantly reducing myocardial oxygen consumption. Modern research shows that the components of Panax notoginseng mainly include various physiologically active substances such as Panax notoginseng total saponins, volatile oils, amino acids, Panax notoginseng flavonoids, Panax notoginseng polysaccharides, etc. Among them, Panax notoginseng total saponins are the main effective active ingredients of Panax notoginseng, which can improve memory and physical resistance, and also have the effect of protecting the liver. Volatile oil has a sedative and tranquilizing effect, and amino acids are essential components for the human body and are very good for the body. Flavonoids can improve blood microcirculation, and Panax notoginseng polysaccharides have a promoting immune effect.

[0028] The inflated fruit liquorice described in the present invention belongs to a variety of liquorice. Licorice is also known as sweet grass root, powder grass, Lingtong, Guolao, Meicao, honey sweet, soft gold, etc. It is a perennial herb belonging to the genus Glycyrrhiza L. of the Leguminosae family. At present, there are 29 species and 6 variants in the world, and 18 species and 3 variants in my country. The history of using liquorice in traditional Chinese medicine has a long history. As early as in the "Shennong Bencao Jing", it was listed as a "top grade". "The Collection of Notes on the Classic of Materia Medica" records that "this grass is the king of all grasses, and few prescriptions do not use it". Li Shizhen's "Compendium of Materia Medica" records that liquorice can "coordinate all products, have the merit of the elders... It can be said to be a good minister in medicine". Zou's "Benjing Shuzheng" in the Qing Dynasty said: "In the two books "Treatise on Febrile Diseases" and "Golden Chamber Synopsis", there are 250 prescriptions, and 120 prescriptions use liquorice". Its wide application and great effect can be seen. The 2020 edition of the Chinese Pharmacopoeia contains three varieties of licorice: Glycyrrhiza uralensis Fisch., Glycyrrhiza glabra L., and Glycyrrhiza inflata Bat. It is reported that licorice polysaccharide, as an important biologically active ingredient in Glycyrrhiza inflata, can control and regulate the division, differentiation, growth and aging of body cells, and has multiple immune enhancement functions and strong anti-tumor and antiviral effects. Example 1

[0029] A glycyrrhiza inflata anti-allergic soothing solution comprises the following components: rhodiola rosea root extract, PANAX notoginseng root extract and glycyrrhiza inflata root extract.

[0030] The preparation process of the Glycyrrhiza inflata anti-allergic soothing solution comprises the following specific steps: Step (1): weigh the following raw materials in parts by weight: 1 part of Rhodiola rosea root, 10 parts of Panax notoginseng root, and 1 part of Glycyrrhiza inflata root; heat and dry until the water content is less than 1.5%, and grind to obtain a composite powder; weigh anhydrous ethanol 10 times the weight of the composite powder, slowly stir at room temperature for 10 hours, then centrifuge to obtain a precipitate, and then rotary dry to remove the ethanol to obtain a defatted product; Step (2): adding 10 times the weight of water to the above-mentioned defatted product, and adding a mixture of neutral cellulase and protease to perform enzymolysis, specifically: adjusting the pH value to 7, the temperature to 46°C, and stirring for 4 hours; wherein the amount of neutral cellulase added is 1% of the dry weight of the defatted product, and the amount of protease added is 0.1% of the dry weight of the defatted product; obtaining an enzymolysis product; Step (3): heating to 100°C, keeping warm and stirring for 1 hour; then centrifuging at 5000 rpm for 10 minutes, and taking the supernatant; Step (4): Concentrate the supernatant by rotary evaporation to 1 / 20 of the original volume, then add 4 times the volume of anhydrous ethanol, let it stand at 5°C for 12 hours, centrifuge at 5000 rpm for 10 minutes, collect the precipitate; then add 20 times the weight of the precipitate in deionized water, stir and dissolve, and obtain the Glycyrrhiza uralensis anti-allergic soothing solution. Example 2

[0031] A glycyrrhiza inflata anti-allergic soothing solution comprises the following components: rhodiola rosea root extract, PANAX notoginseng root extract and glycyrrhiza inflata root extract.

[0032] The preparation process of the Glycyrrhiza inflata anti-allergic soothing solution comprises the following specific steps: Step (1): weigh the following raw materials in parts by weight: 10 parts of rhodiola rosea root, 1 part of Panax notoginseng root, and 10 parts of Glycyrrhiza inflata root; heat and dry until the water content is less than 1.5%, and grind to obtain a composite powder; weigh anhydrous ethanol 5 times the weight of the composite powder, slowly stir at room temperature for 20 hours, then centrifuge to obtain a precipitate, and then rotary evaporate to dry, remove the ethanol, and obtain a defatted product; Step (2): adding 5 times the weight of water to the above-mentioned defatted product, and adding a mixture of neutral cellulase and protease to perform enzymolysis, specifically: adjusting the pH value to 8, the temperature to 42°C, and stirring for 8 hours; wherein the amount of neutral cellulase added is 0.1% of the dry weight of the defatted product, and the amount of protease added is 1% of the dry weight of the defatted product; obtaining an enzymolysis product; Step (3): heating to 95°C, keeping warm and stirring for 2 hours; then centrifuging at 3000 rpm for 30 minutes, and taking the supernatant; Step (4): Concentrate the supernatant by rotary evaporation to 1 / 10 of the original volume, then add 5 times the volume of anhydrous ethanol, let it stand at 1°C for 24 hours, centrifuge at 3000 rpm for 30 minutes, collect the precipitate; then add 10 times the weight of the precipitate in deionized water, stir and dissolve, and obtain the Glycyrrhiza uralensis anti-allergic soothing solution. Example 3

[0033] A glycyrrhiza inflata anti-allergic soothing solution comprises the following components: rhodiola rosea root extract, PANAX notoginseng root extract and glycyrrhiza inflata root extract.

[0034] The preparation process of the Glycyrrhiza inflata anti-allergic soothing solution comprises the following specific steps: Step (1): weigh the following raw materials in parts by weight: 5 parts of Rhodiola rosea root, 3 parts of Panax notoginseng root, and 8 parts of Glycyrrhiza inflata root; heat and dry until the water content is less than 1.5%, and grind to obtain a composite powder; weigh anhydrous ethanol in an amount of 7 times the weight of the composite powder, slowly stir at room temperature for 15 hours, then centrifuge to obtain a precipitate, and then rotary evaporate to dry, remove the ethanol, and obtain a defatted product; Step (2): adding 8 times the weight of water to the above-mentioned defatted product, and adding a mixture of neutral cellulase and protease to perform enzymolysis, specifically: adjusting the pH value to 7.5, the temperature to 43°C, and stirring for 6 hours; wherein the amount of neutral cellulase added is 0.5% of the dry weight of the defatted product, and the amount of protease added is 0.5% of the dry weight of the defatted product; obtaining an enzymolysis product; Step (3): heating to 98°C, keeping warm and stirring for 1.5 hours; then centrifuging at 4000 rpm for 20 minutes, and collecting the supernatant; Step (4): Concentrate the supernatant by rotary evaporation to 1 / 15 of the original volume, then add 4.5 times the volume of anhydrous ethanol, let it stand at 3°C ​​for 16 hours, centrifuge at 4000 rpm for 20 minutes, collect the precipitate; then add 15 times the weight of the precipitate in deionized water, stir and dissolve, and obtain the Glycyrrhiza uralensis anti-allergic soothing solution.

[0035] Test 1 The extraction process of the present invention adopts the method of defatting and enzymolysis. The two processes complement each other. Compared with a single extraction method, the purpose is to improve the extraction efficiency of active substances such as small molecular polysaccharides. A comparison group A using only the defatting method and a comparison group B using only the enzymolysis method are specially set. The other processes of the comparison groups A and B are the same as those in Example 3.

[0036] Comparative group A: a Glycyrrhiza inflata anti-allergic soothing solution, comprising the following components: Rhodiola rosea root extract, Panax notoginseng root extract, and Glycyrrhiza inflata root extract.

[0037] The preparation process of the Glycyrrhiza inflata anti-allergic soothing solution comprises the following specific steps: Step (1): weigh the following raw materials in parts by weight: 5 parts of Rhodiola rosea root, 3 parts of Panax notoginseng root, and 8 parts of Glycyrrhiza inflata root; heat and dry until the water content is less than 1.5%, and grind to obtain a composite powder; Step (2): adding 8 times the weight of water to the above composite powder, and adding a mixture of neutral cellulase and protease to perform enzymolysis, specifically: adjusting the pH value to 7.5, the temperature to 43°C, and stirring for 6 hours; wherein the amount of neutral cellulase added is 0.5% of the dry weight of the defatted product, and the amount of protease added is 0.5% of the dry weight of the defatted product; obtaining an enzymolysis product; Step (3): heating to 98°C, keeping warm and stirring for 1.5 hours; then centrifuging at 4000 rpm for 20 minutes, and collecting the supernatant; Step (4): Concentrate the supernatant by rotary evaporation to 1 / 15 of the original volume, then add 4.5 times the volume of anhydrous ethanol, let it stand at 3°C ​​for 16 hours, centrifuge at 4000 rpm for 20 minutes, collect the precipitate; then add 15 times the weight of the precipitate in deionized water, stir and dissolve, and obtain the Glycyrrhiza uralensis anti-allergic soothing solution.

[0038] Comparative group B: A Glycyrrhiza inflata anti-allergic soothing solution, characterized in that it comprises the following components: Rhodiola rosea root extract, Panax notoginseng root extract, and Glycyrrhiza inflata root extract.

[0039] The preparation process of the Glycyrrhiza inflata anti-allergic soothing solution comprises the following specific steps: Step (1): weigh the following raw materials in parts by weight: 5 parts of Rhodiola rosea root, 3 parts of Panax notoginseng root, and 8 parts of Glycyrrhiza inflata root; heat and dry until the water content is less than 1.5%, and grind to obtain a composite powder; weigh anhydrous ethanol in an amount of 7 times the weight of the composite powder, slowly stir at room temperature for 15 hours, then centrifuge to obtain a precipitate, and then rotary evaporate to dry, remove the ethanol, and obtain a defatted product; Step (2): add 8 times the weight of water to the above-mentioned defatted product, raise the temperature to 98°C, and stir for 1.5 hours; then centrifuge at 4000 rpm for 20 minutes and take the supernatant; Step (3): Concentrate the supernatant by rotary evaporation to 1 / 15 of the original volume, then add 4.5 times the volume of anhydrous ethanol, let it stand at 3°C ​​for 16 hours, centrifuge at 4000 rpm for 20 minutes, collect the precipitate; then add 15 times the weight of the precipitate in deionized water, stir and dissolve, and obtain the Glycyrrhiza uralensis anti-allergic soothing solution.

[0040] The purpose of this experiment is to determine the content of polysaccharides and total sugars in the anti-allergic soothing solution of Glycyrrhiza uralensis obtained in Example 3, Comparative Group A, and Comparative Group B. The specific test method refers to the literature "Mukaidais Maihemuti, Chen Jian, Jiao Chunwei, et al. Study on the antioxidant whitening and cell proliferation effects of Ganoderma lucidum extract [J]. World Traditional Chinese Medicine, 2024, 19(07): 949-956.". The specific test results are shown in the attached Figure 1 .

[0041] By the attached Figure 1 It can be seen that the total sugar content of the Glycyrrhiza inflata anti-allergic soothing solution prepared in Example 3 of the present invention reached 55.39 mg / g, and the polysaccharide content reached 29.18 mg / g. Compared with the comparison group A using only the defatting method and the comparison group B using only the enzymatic hydrolysis method, the extraction efficiency of total sugar, especially polysaccharide, was greatly improved.

[0042] Test 2 Lipopolysaccharide (LSP) is the most common and harmful inflammatory factor. It is composed of four parts: hydrophilic O-specific antigen polysaccharide, lipid A, core polysaccharide and specific side chain. Among them, lipid A is composed of glucosamine, phosphate and fatty acid, which is the toxic center and main biologically active part of LSP. The mechanism by which LSP acts on RAW264.7 to induce inflammatory response is to form LSP-LBP-SCD14 complex to act on TLR4 and activate NF-κB and MAPKs signaling pathways, induce the mRNA expression of iNOS and COX-2 in RAW264.7 macrophages, promote the transcription of inflammatory genes iNOS and COX-2, and increase the release of proinflammatory cytokines such as TNF-α, IL-6 and IL-1β.

[0043] This experiment was conducted in accordance with the method described in the literature "Wang Xuewen, Liu Hui, Wang Qian, et al. Dipotassium glycyrrhizinate promotes skin repair by regulating macrophage polarization [J]. Surfactant Chemical Industry (Chinese and English), 2024, 54(11): 1355-1361."

[0044] This experiment 2 verifies the effect of the Glycyrrhiza uralensis anti-allergic soothing solution prepared in Examples 1-3, and sets up comparisons 1-4 in order to explore the interaction between the raw materials used in this application and the difference between the process used and the conventional water extraction method.

[0045] Comparative Example 1 An anti-allergic soothing solution, characterized in that it comprises the following components: Rhodiola rosea (RHODIOLA ROSEA) root extract.

[0046] The preparation process of the anti-allergic soothing solution comprises the following specific steps: Step (1): weigh the following raw materials in parts by weight: 16 parts of Rhodiola rosea root; heat and dry until the water content is less than 1.5%, and crush to obtain a composite powder; weigh anhydrous ethanol 7 times the weight of the composite powder, slowly stir at room temperature for 15 hours, then centrifuge to obtain a precipitate, and then rotary evaporate to dry, remove the ethanol, and obtain a defatted product; Step (2): adding 8 times the weight of water to the above-mentioned defatted product, and adding a mixture of neutral cellulase and protease to perform enzymolysis, specifically: adjusting the pH value to 7.5, the temperature to 43°C, and stirring for 6 hours; wherein the amount of neutral cellulase added is 0.5% of the dry weight of the defatted product, and the amount of protease added is 0.5% of the dry weight of the defatted product; obtaining an enzymolysis product; Step (3): heating to 98°C, keeping warm and stirring for 1.5 hours; then centrifuging at 4000 rpm for 20 minutes, and collecting the supernatant; Step (4): Concentrate the supernatant by rotary evaporation to 1 / 15 of the original volume, then add 4.5 times the volume of anhydrous ethanol, let it stand at 3°C ​​for 16 hours, centrifuge at 4000 rpm for 20 minutes, collect the precipitate; then add 15 times the weight of the precipitate in deionized water, stir and dissolve, and obtain an anti-allergic soothing solution.

[0047] Comparative Example 2 An anti-allergic soothing solution, characterized by comprising the following components: Panax notoginseng root extract.

[0048] The preparation process of the anti-allergic soothing solution comprises the following specific steps: Step (1): Weigh the following raw materials in parts by weight: 16 parts of Panax notoginseng root; heat and dry until the water content is less than 1.5%, and crush to obtain a composite powder; weigh anhydrous ethanol 7 times the weight of the composite powder, slowly stir at room temperature for 15 hours, then centrifuge to obtain a precipitate, and then rotary dry to remove the ethanol to obtain a defatted product; Step (2): adding 8 times the weight of water to the above-mentioned defatted product, and adding a mixture of neutral cellulase and protease to perform enzymolysis, specifically: adjusting the pH value to 7.5, the temperature to 43°C, and stirring for 6 hours; wherein the amount of neutral cellulase added is 0.5% of the dry weight of the defatted product, and the amount of protease added is 0.5% of the dry weight of the defatted product; obtaining an enzymolysis product; Step (3): heating to 98°C, keeping warm and stirring for 1.5 hours; then centrifuging at 4000 rpm for 20 minutes, and collecting the supernatant; Step (4): Concentrate the supernatant by rotary evaporation to 1 / 15 of the original volume, then add 4.5 times the volume of anhydrous ethanol, let it stand at 3°C ​​for 16 hours, centrifuge at 4000 rpm for 20 minutes, collect the precipitate; then add 15 times the weight of the precipitate in deionized water, stir and dissolve, and obtain an anti-allergic soothing solution.

[0049] Comparative Example 3 An anti-allergic soothing solution, characterized in that it comprises the following components: Glycyrrhiza inflata (Glycyrrhiza inflata) root extract.

[0050] The preparation process of the anti-allergic soothing solution comprises the following specific steps: Step (1): Weigh the following raw materials in parts by weight: 16 parts of Glycyrrhiza inflata root; heat and dry until the water content is less than 1.5%, and crush to obtain a composite powder; weigh anhydrous ethanol 7 times the weight of the composite powder, slowly stir at room temperature for 15 hours, then centrifuge to obtain a precipitate, and then rotary dry to remove the ethanol to obtain a defatted product; Step (2): adding 8 times the weight of water to the above-mentioned defatted product, and adding a mixture of neutral cellulase and protease to perform enzymolysis, specifically: adjusting the pH value to 7.5, the temperature to 43°C, and stirring for 6 hours; wherein the amount of neutral cellulase added is 0.5% of the dry weight of the defatted product, and the amount of protease added is 0.5% of the dry weight of the defatted product; obtaining an enzymolysis product; Step (3): heating to 98°C, keeping warm and stirring for 1.5 hours; then centrifuging at 4000 rpm for 20 minutes, and collecting the supernatant; Step (4): Concentrate the supernatant by rotary evaporation to 1 / 15 of the original volume, then add 4.5 times the volume of anhydrous ethanol, let it stand at 3°C ​​for 16 hours, centrifuge at 4000 rpm for 20 minutes, collect the precipitate; then add 15 times the weight of the precipitate in deionized water, stir and dissolve, and obtain an anti-allergic soothing solution.

[0051] Comparative Example 4 An anti-allergic soothing solution, characterized in that it comprises the following components: Rhodiola rosea root extract, Panax notoginseng root extract, and Glycyrrhiza inflata root extract.

[0052] The preparation process of the anti-allergic soothing solution comprises the following specific steps: Step (1): weigh the following raw materials in parts by weight: 5 parts of Rhodiola rosea root, 3 parts of Panax notoginseng root, and 8 parts of Glycyrrhiza inflata root; heat and dry until the water content is less than 1.5%, and grind to obtain a composite powder; Step (2): add 8 times the weight of water to the above composite powder, raise the temperature to 98°C, keep warm and stir for 1.5 hours; then centrifuge at 4000 rpm for 20 minutes, and take the supernatant; Step (3): Concentrate the supernatant by rotary evaporation to 1 / 15 of the original volume, then add 4.5 times the volume of anhydrous ethanol, let it stand at 3°C ​​for 16 hours, centrifuge at 4000 rpm for 20 minutes, collect the precipitate; then add 15 times the weight of the precipitate in deionized water, stir and dissolve, and obtain the anti-allergic soothing solution.

[0053] Specific test steps of this experiment 2: RAW264.7 cells were cultured in DMEM high-glucose complete medium (containing 89% DMEM high-glucose medium, 10% fetal bovine serum, and 1% double antibody). Cell fusion reached 80% for passage and freezing, usually 2-3 days per passage. Cells in logarithmic growth phase and in good condition were taken for experiment; blank control group (BC), negative control group (NC, 1µg / mL LPS), positive control group (PC, 1 µg / mL LPS + 0.08μmoL / mL dexamethasone) and sample group (1µg / mL LPS + 0.01% test sample) were set up in the experiment, with 3 replicates in each group; RAW264.7 cells were seeded in 6-well plates at a cell density of 1×105 / mL (1 mL per well). Overnight, drug treatment. After 24 hours, the supernatant was aspirated and centrifuged at 12000 r / min for 10 minutes. The supernatant was taken and packaged according to the experimental requirements. The collected RAW264.7 cell supernatant was placed at room temperature to melt and the sample was mixed. During the experiment, the experiments were carried out according to the requirements of the ELISA kits for TNF-α and IL-6. The specific test results are shown in Table 1.

[0054] Table 1 Anti-allergic soothing solution regulates the secretion of cytokines

[0055] As can be seen from Table 1, the anti-allergic soothing solution prepared in the embodiment of the present invention can regulate the secretion of inflammatory mediators and inflammatory factors and exert an anti-inflammatory effect.

[0056] Example 4 A sensitive skin soothing cream is composed of the following components in percentage by weight: 2% glycerin, 0.25% ammonium acryloyldimethyltaurate / VP copolymer, 0.5% acrylic acid (esters) / C10-30 alkyl acrylate crosspolymer, 0.1% xanthan gum, 0.05% sodium hyaluronate, 0.25% allantoin, 0.5% p-hydroxyacetophenone, 0.35% PEG-20 methyl glucose sesquistearate, 0.5% tocopheryl acetate, 1.7% polydimethylsiloxane, 2% PEG / PPG-17 / 6 copolymer, 0.7% 1,2-hexanediol, 0.5% triethanolamine, 4% glycyrrhiza inflata anti-allergic soothing solution prepared in Example 3, and the balance water.

[0057] Test 3 The sensitive skin soothing cream prepared in Example 4 was tested on volunteers. Specifically refer to the method described in the document "Liao Zhigang, Wen Si, Xian Xuesong, et al. Application of repairing and soothing functional skin care products in improving sensitive skin: based on a cohort study of 60 cases [J]. Journal of Dermatology and Venereology, 2023, 30(06): 495-501."

[0058] 20 volunteers with sensitive skin were recruited in the factory. Inclusion criteria: 18-45 years old, self-assessed as sensitive skin (self-perceived allergic problems such as redness, dryness, itching, burning, tingling, etc.), and positive lactic acid stimulation test. Exclusion criteria: patients with uncured clinical inflammatory skin diseases; patients who have received cosmetic treatment or used topical drugs on their face in the past 3 months; patients with acute or chronic systemic diseases.

[0059] After cleaning their face every day, volunteers took an appropriate amount of sensitive skin soothing cream and evenly applied it on their face, gently massaged until absorbed, once in the morning and evening, for 28 consecutive days. Two hours after the first use of the test sample and on the 28th day, the subjects cleaned their faces with a mild facial cleanser under the guidance of the researchers, and dried the water with a crumb-free facial tissue. After sitting in the test environment (temperature 20-22℃, humidity 40-60%) for 30 minutes, the researchers evaluated the efficacy of the subjects.

[0060] Evaluation 1: Lactic acid stinging test: 50μL of 10% lactic acid solution was dropped on a filter paper and then attached to the nasolabial groove on either side of the subject. At the same time, an equal amount of saline was dropped on a filter paper and then attached to the nasolabial groove on the other side. The subject was asked about his / her subjective symptoms 30s, 2.5min and 5min after application, and the score was given according to the 4-point method. 0 points: no stinging; 1 point: mild stinging; 2 points: moderate stinging; 3 points: severe stinging. Subtract the total scores on both sides. If the difference is ≥3 points, the lactic acid stinging reaction is positive. The specific test results are shown in Table 2.

[0061] Table 2 Lactic acid sting test results

[0062] Evaluation 2: Skin barrier test: The test was conducted using a transepidermal water loss meter. The test area was randomly marked at the intersection of the midline of the eye and the midline of the bridge of the nose on one side of the subject. Each test was performed 3 times in the test area and the average value was taken. The specific test results are shown in Table 3.

[0063] Table 3 Skin barrier test results

[0064] As can be seen from Tables 2 and 3, the TEWL value of the volunteers was reduced after using the sensitive skin soothing cream prepared in the embodiment of the present invention, and the skin barrier was repaired; the sensitivity value to lactic acid and external stimuli was reduced from an average of 3.76 to 2.85. After the test results, the volunteers were questioned and it was learned that most of the volunteers' allergic problems such as redness, dry itching, burning, and tingling were alleviated or cured. It can be seen that the sensitive skin soothing cream prepared in Example 4 of the present invention has achieved good anti-allergic and soothing effects on sensitive skin volunteers.

[0065] The technical features of the above embodiments may be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0066] The above embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for those of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A Glycyrrhiza uralensis anti-allergic soothing liquid, characterized in that: The invention comprises the following components: RHODIOLA ROSEA root extract, PANAX NOTOGINSENG root extract and GLYCYRRHIZA INFLATA root extract.

2. The anti-allergic soothing liquid of Glycyrrhiza inflata according to claim 1, characterized in that: The preparation process of the Glycyrrhiza inflata anti-allergic soothing solution is as follows: Step (1): weighing the following raw materials in parts by weight: 1-10 parts of Rhodiola rosea root, 1-10 parts of Panax notoginseng root, and 1-10 parts of Glycyrrhiza inflata root; drying and crushing to obtain a composite powder; performing a defatting treatment to obtain a defatted product; Step (2): adding 5-10 times the weight of water to the above-mentioned defatted product, and adding enzymes for enzymolysis to obtain an enzymolysis product; Step (3): heating to 95-100°C, keeping warm and stirring for 1-2 hours; then centrifuging at 3000-5000 rpm for 10-30 minutes, and taking the supernatant; Step (4): Concentrate the supernatant by rotary evaporation to 1 / 10-1 / 20 of the original volume, then add 4-5 times the volume of anhydrous ethanol, let it stand at 1-5°C for 12-24 hours, centrifuge at 3000-5000 rpm for 10-30 minutes, collect the precipitate; then add deionized water 10-20 times the weight of the precipitate, stir and dissolve, and obtain the Glycyrrhiza uralensis anti-allergic soothing solution.

3. The anti-allergic soothing liquid of Glycyrrhiza inflata according to claim 2, characterized in that: The weight parts of the raw materials described in step (1) are: 5 parts of Rhodiola rosea root, 3 parts of Panax notoginseng root, and 8 parts of Glycyrrhiza inflata root.

4. The anti-allergic soothing liquid of Glycyrrhiza inflata according to claim 2, characterized in that: The degreasing process described in step (1) is as follows: weigh 5-10 times the weight of the composite powder in anhydrous ethanol, slowly stir at room temperature for 10-20 hours, then centrifuge to obtain a precipitate, and then rotary evaporate to dry, remove the ethanol, and obtain a defatted product.

5. The anti-allergic soothing liquid of Glycyrrhiza inflata according to claim 2, characterized in that: The enzyme described in step (2) is a mixture of neutral cellulase and protease.

6. The Glycyrrhiza inflata anti-allergic soothing solution according to claim 2, characterized in that: The enzymatic hydrolysis process in step (2) is as follows: adjusting the pH value to 7-8, the temperature to 42-46° C., and stirring for 4-8 hours.

7. The anti-allergic soothing liquid of Glycyrrhiza inflata according to claim 2, characterized in that: In step (2), the amount of neutral cellulase added is 0.1-1% of the dry weight of the defatted product, and the amount of protease added is 0.1-1% of the dry weight of the defatted product.

8. Use of the Glycyrrhiza inflata anti-allergic soothing solution according to any one of claims 1 to 7 in the preparation of cosmetics.

9. A sensitive skin soothing cream, characterized in that: The invention comprises the following components in percentage by weight: 2% glycerin, 0.25% ammonium acryloyldimethyltaurate / VP copolymer, 0.5% acrylic acid (esters) / C10-30 alkyl acrylate crosspolymer, 0.1% xanthan gum, 0.05% sodium hyaluronate, 0.25% allantoin, 0.5% p-hydroxyacetophenone, 0.35% PEG-20 methyl glucose sesquistearate, 0.5% tocopheryl acetate, 1.7% polydimethylsiloxane, 2% PEG / PPG-17 / 6 copolymer, 0.7% 1,2-hexanediol, 0.5% triethanolamine, 4% of the anti-allergic soothing solution of Glycyrrhiza inflata according to any one of claims 1 to 7, and the balance water.

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