Centella asiatica plant extraction repairing agent and application thereof in cosmetics

Abstract

The invention discloses a centella asiatica plant extraction repairing agent and application thereof in cosmetics. The centella asiatica plant extraction repairing agent comprises the following components: an olive leaf extract, a centella asiatica root extract and an oat kernel extract. According to the preparation process of the centella asiatica plant extraction repairing agent, plant water-soluble active ingredients are obtained by adopting enzymolysis and alcohol extraction modes, plant oil-soluble active ingredients are obtained by adopting critical CO2 extraction, and then a novel preparation is prepared by adopting an ultrasonic emulsification technology, so that the repairing agent can permeate into skin cuticles more easily, the effect of active matters can be brought into full play, and the repairing agent has a good application prospect. When the composition is applied to cosmetics, a good repairing effect can be realized.

Description

Technical Field

[0001] The invention relates to the field of cosmetics, and in particular to a Centella asiatica plant extract repairing agent and application thereof in cosmetics. Background Art

[0002] The skin barrier is the main physical barrier between the human body and the external environment. Its function is to prevent external substances from entering the body to damage tissues and stimulate immune responses. Factors that damage the skin barrier function include endogenous factors, exogenous factors, and unhealthy lifestyles. Common endogenous factors include gender, genetics, age, etc.; exogenous factors include air pollution, seasonal changes, sunlight, and temperature changes. Excessive or long-term exposure to the above conditions can damage the skin barrier, causing skin dehydration and inflammation, manifested as sagging, redness, swelling, peeling, pain, etc. Therefore, repairing skin barrier damage has become one of people's important needs.

[0003] The definition of the repair effect claim in the Cosmetics Classification Catalogue is that it helps maintain the normal state of the application site. Maintaining the normal state of the skin means maintaining the normal structure and function of the skin. When the skin is damaged, the structure and function of the skin will change, and adverse reactions such as weakened cell vitality, damage to the extracellular matrix structure, immune response and weakened skin barrier function may occur.

[0004] Oats are nutritionally balanced functional foods. They are rich in polyphenols, flavonoids, alkaloids, β-glucan and other active ingredients. They have multiple medicinal and health functions, making them widely used in food and non-food fields (pharmaceutical products, detergents, papermaking, cosmetics, etc.).

[0005] The main active ingredients in olive leaves include compounds such as phenylpropanoids, flavonoids, pentacyclic triterpenes and secoiridoids, among which flavonoids and secoiridoids account for the largest proportion. The flavonoids in olive leaves mainly include oleuropein, apigenin-7-O-glucoside, catechins and eriodictyol, and the secoiridoids include oleuropein and have the highest content in olive leaves. Olive leaves have a wide range of pharmacological effects, such as lowering blood lipids, lowering blood sugar, lowering blood pressure, anti-oxidation, anti-ischemia, anti-cancer, anti-inflammatory, anti-viral, etc. Polyphenol compounds are the most abundant secondary metabolites in plants and are the main source of pharmacological effects such as antioxidant, anti-inflammatory, antibacterial, and anti-cancer in plants. Olive leaves are rich in polyphenols. In recent years, olive leaves have been widely used in medicine, nutritional health products, agriculture, food, and daily cosmetics.

[0006] Centella asiatica, a Chinese medicine, is the dried whole herb of the plant Centella asiatica of the Apiaceae family. It is bitter, pungent and cold. It has the effects of clearing away heat and dampness, detoxifying and reducing swelling. It can be used as an anti-inflammatory agent to improve the healing of minor wounds, burns, and hypertrophic wounds. It is also recommended as an antipyretic, diuretic, rheumatic, antibacterial, antiviral drug for the treatment of venous insufficiency, improving cognition, relieving anxiety and anticancer drug.

[0007] After extensive research in the field of cosmetics, the inventors of the present invention discovered that a Centella asiatica plant extract repair agent was obtained by combining oats, olive leaves and Centella asiatica in a very specific range of proportions and extracting them through a specific process. The Centella asiatica plant extract repair agent can effectively promote skin metabolism and enhance skin barrier capacity. The application of the extract in cosmetics can have a good repair effect, thus completing the present invention. Summary of the invention

[0008] The purpose of the present invention is to provide a Centella asiatica plant extract repair agent, which can effectively promote skin metabolism, enhance skin barrier ability, and have a good repair effect on damaged skin.

[0009] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions: A Centella asiatica plant extract repair agent, characterized in that it comprises the following components: olive (OLEA EUROPAEA) leaf extract, Centella asiatica (CENTELLA ASIATICA) root extract, and oat (AVENA SATIVA) kernel extract.

[0010] The embodiment of the present invention discloses a preparation process of the Centella asiatica plant extract repair agent, comprising the following steps: (1) Weigh the following raw materials in parts by weight: 1-10 parts of olive leaves, 1-10 parts of Centella asiatica roots, and 1-10 parts of oat kernels, and grind and sieve to obtain a mixed powder; (2) mixing the mixed powder, water and cellulase, stirring and hydrolyzing; adding ethanol; soaking and extracting under stirring, filtering, and obtaining a filtrate and a filter residue; (3) Add an equal volume of glycerol to the filtrate, remove the ethanol by rotary evaporation, and centrifuge to obtain the supernatant; (4) drying the filter residue and extracting it with supercritical CO2 for 2-3 hours to obtain an oily extract; (5) Heat Tween 80, oily extract, lecithin, and sucrose cocoate to dissolve, and slowly drop the clear liquid into the mixture while stirring; then use an ultrasonic homogenizer to process the mixture three times, and filter the mixture with a filter membrane to obtain the Centella asiatica plant extract repair agent.

[0011] In some embodiments, the weight parts of the raw materials in step (1) are: 6 parts of olive leaves, 3 parts of Centella asiatica roots, and 7 parts of oat kernels.

[0012] In some embodiments, the mass ratio of the mixed powder, water and cellulase in step (2) is 1:5-10:0.01-0.05.

[0013] In some embodiments, the stirring hydrolysis condition in step (2) is: stirring hydrolysis at 50-55° C. for 3-5 hours.

[0014] In some of the embodiments, ethanol is added to the system in step (2) to make the volume concentration of ethanol reach 30-50%.

[0015] In some of the embodiments, the process conditions of the supercritical CO2 extraction in step (4) are: extraction temperature 55°C, extraction pressure 30MPa, and CO2 circulation volume 0.6kg / g.

[0016] In some embodiments, the mass ratio of Tween 80, oily extract, lecithin, sucrose cocoate, and supernatant in step (5) is 1-5:1-10:1-5:0.1-1:50-80.

[0017] In some embodiments, the filter membrane in step (5) is a 0.22 μm organic microporous filter membrane.

[0018] The Centella asiatica plant extract repair agent prepared in the embodiment of the present invention can be used as an active ingredient in a cosmetic formula, and its addition amount can be 1-10wt%. The cosmetic can be in the form of cream, lotion, facial mask, essence, facial cleanser, etc.

[0019] The embodiment of the present invention also provides a plant extract repair essence, which is composed of the following raw materials in percentage by weight: 3% glycerin, 7% butylene glycol, 0.03% disodium EDTA, 0.15% acrylates / C10-30 alkyl acrylate cross-linked polymer, 0.25% allantoin, 0.1% sodium hyaluronate, 0.5% panthenol, 0.12% xanthan gum, 0.08% hydroxyethyl cellulose, 0.12% methylparaben, 2% PEG / PPG-17 / 6 copolymer, 2% betaine, 0.05% caprylhydroxamic acid, 0.15% triethanolamine, 1-10% of the above-mentioned Centella asiatica plant extract repair agent, and the balance water.

[0020] The technical solution provided by the embodiment of the present invention has the following beneficial effects: the Centella asiatica plant extract repairing agent prepared by the present invention adopts a plant formula, and its effect is better than that of a single formula preparation; the preparation process of the Centella asiatica plant extract repairing agent of the present invention adopts enzymatic hydrolysis and alcohol extraction to obtain plant water-soluble active ingredients, critical CO2 extraction to obtain plant oil-soluble active ingredients, and then lecithin and sucrose cocoate are added, and ultrasonic emulsification technology is used to prepare a stable alcohol body preparation, which is easier to penetrate into the stratum corneum of the skin, which is conducive to giving full play to the repair effect of the active substance, can effectively promote skin metabolism, enhance skin barrier ability, and has a good repair effect on damaged skin. Applying it to cosmetics can achieve a good repair effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for describing the embodiments or the prior art are briefly introduced below.

[0022] Figure 1 The TEWL value results of each embodiment of the test 2 are shown. DETAILED DESCRIPTION

[0023] The embodiments in the specification are only used to illustrate the present invention and do not limit the scope of protection of the present invention. The scope of protection of the present invention is only limited by the claims, and any omission, replacement or modification made on the basis of the embodiments disclosed in the present invention shall fall within the scope of protection of the present invention.

[0024] The content of the present invention can be more easily understood by selecting the following detailed description of the preferred implementation method of the present invention and the embodiments included. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those of ordinary skill in the art to which the present invention belongs. When there is a conflict, the definition in this specification shall prevail.

[0025] As used herein, the term "prepared from" is synonymous with "comprising." As used herein, the terms "comprising," "including," "having," "containing," or any other variation thereof, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises the listed elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

[0026] When amount, concentration or other value or parameter is expressed as range, preferred range or a series of upper preferred value and lower preferred value limit range, this should be understood as specifically disclosing all ranges formed by any pairing of any range upper limit or preferred value and any range lower limit or preferred value, regardless of whether the range is disclosed separately. For example, when disclosing range "1 to 5", described range should be interpreted as including range "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5" etc. When numerical range is described in this article, unless otherwise stated, the range is intended to include its end value and all integers and fractions within the range.

[0027] In addition, the indefinite articles "a" and "an" before the elements or components of the present invention have no restrictions on the quantity requirements (i.e. the number of occurrences) of the elements or components. Therefore, "a" or "an" should be interpreted as including one or at least one, and the elements or components in the singular form also include the plural form, unless the number is obviously intended to be in the singular form.

[0028] In order to deepen the understanding of the present invention, the present invention will be further described in detail below in conjunction with examples. The examples are only used to explain the present invention and do not constitute a limitation on the protection scope of the present invention.

[0029] Example 1 (1) Weigh the following raw materials in parts by weight: 1 part of olive leaves, 10 parts of Centella asiatica roots, and 1 part of oat kernels, and grind them through a 50-mesh sieve to obtain a mixed powder; (2) Mix the mixed powder, water and cellulase in a mass ratio of 1:10:0.01, and stir and hydrolyze at 55°C for 3 hours; add ethanol to the system to make the ethanol volume concentration reach 50%; soak and extract for 5 hours under stirring, and filter with a 1000 mesh filter cloth to obtain a filtrate and a filter residue; (3) Add an equal volume of glycerol to the filtrate, remove the ethanol by rotary evaporation, centrifuge at 5000 rpm for 30 minutes, and collect the supernatant; (4) drying the filter residue, and extracting it with supercritical CO2 for 3 hours at an extraction temperature of 55°C, an extraction pressure of 30 MPa, and a CO2 circulation rate of 0.6 kg / g to obtain an oily extract; (5) Heat and dissolve Tween 80, oily extract, lecithin, and sucrose cocoate, and slowly dropwise add the supernatant while stirring, wherein the mass ratio of Tween 80, oily extract, lecithin, sucrose cocoate, and supernatant is 1:10:1:1:50; then treat with an ultrasonic homogenizer for 3 times, and filter with a 0.22 μm organic microporous filter membrane to obtain the Centella asiatica plant extract repair agent.

[0030] Example 2 A Centella asiatica plant extract repair agent is prepared according to the following preparation process: (1) Weigh the following raw materials in parts by weight: 10 parts of olive leaves, 1 part of Centella asiatica roots, and 10 parts of oat kernels, and grind them through a 50-mesh sieve to obtain a mixed powder; (2) Mix the mixed powder, water and cellulase in a mass ratio of 1:5:0.05, and stir and hydrolyze at 50°C for 5 hours; add ethanol to the system to make the ethanol volume concentration reach 30%; soak and extract for 5 hours under stirring, and filter with a 1000 mesh filter cloth to obtain a filtrate and a filter residue; (3) Add an equal volume of glycerol to the filtrate, remove the ethanol by rotary evaporation, centrifuge at 5000 rpm for 30 minutes, and collect the supernatant; (4) drying the filter residue, extracting it with supercritical CO2 for 2 hours at an extraction temperature of 55°C, an extraction pressure of 30 MPa, and a CO2 circulation rate of 0.6 kg / g to obtain an oily extract; (5) Heat Tween 80, oily extract, lecithin, and sucrose cocoate to dissolve, and slowly drop the supernatant under stirring, wherein the mass ratio of Tween 80, oily extract, lecithin, sucrose cocoate, and supernatant is 5:1:5:0.1:80; then treat with an ultrasonic homogenizer for 3 times, and filter with a 0.22 μm organic microporous filter membrane to obtain the Centella asiatica plant extract repair agent.

[0031] Example 3 A Centella asiatica plant extract repair agent is prepared according to the following preparation process: (1) Weigh the following raw materials in parts by weight: 6 parts of olive leaves, 3 parts of Centella asiatica roots, and 7 parts of oat kernels, and grind them through a 50-mesh sieve to obtain a mixed powder; (2) Mix the mixed powder, water and cellulase in a mass ratio of 1:8:0.03, and stir and hydrolyze at 52°C for 4 hours; add ethanol to the system to make the ethanol volume concentration reach 40%; soak and extract for 5 hours under stirring, and filter with a 1000-mesh filter cloth to obtain a filtrate and a filter residue; (3) Add an equal volume of glycerol to the filtrate, remove the ethanol by rotary evaporation, centrifuge at 5000 rpm for 30 minutes, and collect the supernatant; (4) drying the filter residue, extracting it with supercritical CO2 for 2 hours at an extraction temperature of 55°C, an extraction pressure of 30 MPa, and a CO2 circulation rate of 0.6 kg / g to obtain an oily extract; (5) Heat Tween 80, oily extract, lecithin, and sucrose cocoate to dissolve, and slowly drop the supernatant under stirring, wherein the mass ratio of Tween 80, oily extract, lecithin, sucrose cocoate, and supernatant is 3:5:3:0.5:70; then treat with an ultrasonic homogenizer for 3 times, and filter with a 0.22 μm organic microporous filter membrane to obtain the Centella asiatica plant extract repair agent.

[0032] Test 1: Stability Test Cosmetic preparations should have a reasonable shelf life, which is usually 2-3 years. The industry usually uses accelerated aging tests for heat and cold resistance to ensure their shelf life. Specifically, the test object is placed in a 45℃ or -15℃ environment for 1 month to observe whether it has any abnormalities such as stratification, demulsification, discoloration, or taste change.

[0033] The Centella asiatica plant extract repair agent prepared in the embodiment of the present invention is an alcohol body, and the combination of its emulsifier is very critical to the stability of the system. In order to examine the combination of the emulsifier, comparative examples 1-2 are specially set.

[0034] Comparative Example 1 A Centella asiatica plant extract repair agent is prepared according to the following preparation process: (1) Weigh the following raw materials in parts by weight: 6 parts of olive leaves, 3 parts of Centella asiatica roots, and 7 parts of oat kernels, and grind them through a 50-mesh sieve to obtain a mixed powder; (2) Mix the mixed powder, water and cellulase in a mass ratio of 1:8:0.03, and stir and hydrolyze at 52°C for 4 hours; add ethanol to the system to make the ethanol volume concentration reach 40%; soak and extract for 5 hours under stirring, and filter with a 1000-mesh filter cloth to obtain a filtrate and a filter residue; (3) Add an equal volume of glycerol to the filtrate, remove the ethanol by rotary evaporation, centrifuge at 5000 rpm for 30 minutes, and collect the supernatant; (4) drying the filter residue, extracting it with supercritical CO2 for 2 hours at an extraction temperature of 55°C, an extraction pressure of 30 MPa, and a CO2 circulation rate of 0.6 kg / g to obtain an oily extract; (5) Heat Tween 80, oily extract, and lecithin to dissolve, and slowly drop the supernatant under stirring, wherein the mass ratio of Tween 80, oily extract, lecithin, and supernatant is 3:5:3.5:70; then treat with an ultrasonic homogenizer for 3 times, and filter with a 0.22 μm organic microporous filter membrane to obtain the Centella asiatica plant extract repair agent.

[0035] Comparative Example 2 A Centella asiatica plant extract repair agent is prepared according to the following preparation process: (1) Weigh the following raw materials in parts by weight: 6 parts of olive leaves, 3 parts of Centella asiatica roots, and 7 parts of oat kernels, and grind them through a 50-mesh sieve to obtain a mixed powder; (2) Mix the mixed powder, water and cellulase in a mass ratio of 1:8:0.03, and stir and hydrolyze at 52°C for 4 hours; add ethanol to the system to make the ethanol volume concentration reach 40%; soak and extract for 5 hours under stirring, and filter with a 1000-mesh filter cloth to obtain a filtrate and a filter residue; (3) Add an equal volume of glycerol to the filtrate, remove the ethanol by rotary evaporation, centrifuge at 5000 rpm for 30 minutes, and collect the supernatant; (4) drying the filter residue, extracting it with supercritical CO2 for 2 hours at an extraction temperature of 55°C, an extraction pressure of 30 MPa, and a CO2 circulation rate of 0.6 kg / g to obtain an oily extract; (5) Heat Tween 80, oily extract, and sucrose cocoate to dissolve, and slowly drop the supernatant under stirring, wherein the mass ratio of Tween 80, oily extract, sucrose cocoate, and supernatant is 3:5:3.5:70; then treat with an ultrasonic homogenizer for 3 times, and filter with a 0.22 μm organic microporous filter membrane to obtain the Centella asiatica plant extract repair agent.

[0036] The Centella asiatica plant extract repairing agents prepared in Examples 1-3 and Comparative Examples 1-2 were placed in an environment of 45°C and -15°C for 1 month to observe whether they had abnormalities such as stratification, demulsification, color change, and taste change. The specific test results are shown in Table 1.

[0037] Table 1 Stability test results

[0038] As can be seen from Table 1, the Centella asiatica plant extract repair agent prepared in Examples 1-3 of the present invention has good stability, indicating that the compound use of lecithin and sucrose cocoate plays a key role in the stability of ethosomes.

[0039] Test 2: Efficacy evaluation test The skin barrier usually refers to the outermost layer of the skin, the stratum corneum, which is composed of lipid areas and corneocytes surrounded by it. It prevents the invasion of harmful substances and microorganisms, and prevents excessive loss of water and electrolytes. There are currently a variety of evaluation methods and indicators for evaluating skin barrier damage, such as the hydration state of the stratum corneum, skin pH, lipid composition, etc. The transepidermal water loss (TEWL value) that reflects transepidermal water loss is regarded as the gold standard for evaluating skin barrier function.

[0040] In order to investigate the interaction between the three ingredients of olive leaf, Centella asiatica root and oat kernel in the Centella asiatica plant extract repairing agent prepared by the present invention, and the difference between the preparation process adopted by the present invention and the conventional process, comparative examples 3-6 are specially set up. They are applied in cosmetic formulas, and then human efficacy tests are carried out to investigate the repairing effects of the Centella asiatica plant extract repairing agents prepared by different active ingredients and different processes.

[0041] Comparative Example 3 A Centella asiatica plant extract repair agent is prepared according to the following preparation process: (1) Weigh the following raw materials in parts by weight: 6 parts of olive leaves, 3 parts of Centella asiatica roots, and 7 parts of oat kernels, and grind them through a 50-mesh sieve to obtain a mixed powder; (2) Mix the mixed powder and water in a solid-liquid ratio of 1:15, boil for 30 minutes, centrifuge at 5000 rpm for 30 minutes, and collect the supernatant; (3) Filter the supernatant using a 0.22 μm organic microporous filter membrane to obtain the Centella asiatica plant extract repair agent.

[0042] Comparative Example 4 A Centella asiatica plant extract repair agent is prepared according to the following preparation process: (1) Weigh the following raw materials in parts by weight: 16 parts of olive leaves, crush through a 50-mesh sieve to obtain a mixed powder; (2) Mix the mixed powder, water and cellulase in a mass ratio of 1:8:0.03, and stir and hydrolyze at 52°C for 4 hours; add ethanol to the system to make the ethanol volume concentration reach 40%; soak and extract for 5 hours under stirring, and filter with a 1000-mesh filter cloth to obtain a filtrate and a filter residue; (3) Add an equal volume of glycerol to the filtrate, remove the ethanol by rotary evaporation, centrifuge at 5000 rpm for 30 minutes, and collect the supernatant; (4) drying the filter residue, extracting it with supercritical CO2 for 2 hours at an extraction temperature of 55°C, an extraction pressure of 30 MPa, and a CO2 circulation rate of 0.6 kg / g to obtain an oily extract; (5) Heat Tween 80, oily extract, lecithin, and sucrose cocoate to dissolve, and slowly drop the supernatant under stirring, wherein the mass ratio of Tween 80, oily extract, lecithin, sucrose cocoate, and supernatant is 3:5:3:0.5:70; then treat with an ultrasonic homogenizer for 3 times, and filter with a 0.22 μm organic microporous filter membrane to obtain the Centella asiatica plant extract repair agent.

[0043] Comparative Example 5 A Centella asiatica plant extract repair agent is prepared according to the following preparation process: (1) Weigh the following raw materials in parts by weight: 16 parts of Centella asiatica roots, crush through a 50-mesh sieve to obtain a mixed powder; (2) Mix the mixed powder, water and cellulase in a mass ratio of 1:8:0.03, and stir and hydrolyze at 52°C for 4 hours; add ethanol to the system to make the ethanol volume concentration reach 40%; soak and extract for 5 hours under stirring, and filter with a 1000-mesh filter cloth to obtain a filtrate and a filter residue; (3) Add an equal volume of glycerol to the filtrate, remove the ethanol by rotary evaporation, centrifuge at 5000 rpm for 30 minutes, and collect the supernatant; (4) drying the filter residue, extracting it with supercritical CO2 for 2 hours at an extraction temperature of 55°C, an extraction pressure of 30 MPa, and a CO2 circulation rate of 0.6 kg / g to obtain an oily extract; (5) Heat Tween 80, oily extract, lecithin, and sucrose cocoate to dissolve, and slowly drop the supernatant under stirring, wherein the mass ratio of Tween 80, oily extract, lecithin, sucrose cocoate, and supernatant is 3:5:3:0.5:70; then treat with an ultrasonic homogenizer for 3 times, and filter with a 0.22 μm organic microporous filter membrane to obtain the Centella asiatica plant extract repair agent.

[0044] Comparative Example 6 A Centella asiatica plant extract repair agent is prepared according to the following preparation process: (1) Weigh the following raw materials in parts by weight: 16 parts of oat kernels, crush through a 50-mesh sieve to obtain a mixed powder; (2) Mix the mixed powder, water and cellulase in a mass ratio of 1:8:0.03, and stir and hydrolyze at 52°C for 4 hours; add ethanol to the system to make the ethanol volume concentration reach 40%; soak and extract for 5 hours under stirring, and filter with a 1000-mesh filter cloth to obtain a filtrate and a filter residue; (3) Add an equal volume of glycerol to the filtrate, remove the ethanol by rotary evaporation, centrifuge at 5000 rpm for 30 minutes, and collect the supernatant; (4) drying the filter residue, extracting it with supercritical CO2 for 2 hours at an extraction temperature of 55°C, an extraction pressure of 30 MPa, and a CO2 circulation rate of 0.6 kg / g to obtain an oily extract; (5) Heat Tween 80, oily extract, lecithin, and sucrose cocoate to dissolve, and slowly drop the supernatant under stirring, wherein the mass ratio of Tween 80, oily extract, lecithin, sucrose cocoate, and supernatant is 3:5:3:0.5:70; then treat with an ultrasonic homogenizer for 3 times, and filter with a 0.22 μm organic microporous filter membrane to obtain the Centella asiatica plant extract repair agent.

[0045] Example 4 A plant extract repair essence is composed of the following raw materials in percentage by weight: 3% glycerin, 7% butylene glycol, 0.03% disodium EDTA, 0.15% acrylates / C10-30 alkyl acrylate cross-linked polymer, 0.25% allantoin, 0.1% sodium hyaluronate, 0.5% panthenol, 0.12% xanthan gum, 0.08% hydroxyethyl cellulose, 0.12% methylparaben, 2% PEG / PPG-17 / 6 copolymer, 2% betaine, 0.05% caprylhydroxamic acid, 0.15% triethanolamine, 5% Centella asiatica plant extract repair agent prepared in Example 3, and the balance water.

[0046] Comparative Example 7 A plant extract repair essence is composed of the following raw materials in percentage by weight: 3% glycerin, 7% butylene glycol, 0.03% disodium EDTA, 0.15% acrylates / C10-30 alkyl acrylate crosspolymer, 0.25% allantoin, 0.1% sodium hyaluronate, 0.5% panthenol, 0.12% xanthan gum, 0.08% hydroxyethyl cellulose, 0.12% methylparaben, 2% PEG / PPG-17 / 6 copolymer, 2% betaine, 0.05% caprylhydroxamic acid, 0.15% triethanolamine, 5% Centella asiatica plant extract repair agent prepared in Comparative Example 3, and the balance water.

[0047] Comparative Example 8 A plant extract repair essence is composed of the following raw materials in percentage by weight: 3% glycerin, 7% butylene glycol, 0.03% disodium EDTA, 0.15% acrylates / C10-30 alkyl acrylate crosspolymer, 0.25% allantoin, 0.1% sodium hyaluronate, 0.5% panthenol, 0.12% xanthan gum, 0.08% hydroxyethyl cellulose, 0.12% methylparaben, 2% PEG / PPG-17 / 6 copolymer, 2% betaine, 0.05% caprylhydroxamic acid, 0.15% triethanolamine, 5% Centella asiatica plant extract repair agent prepared in Comparative Example 4, and the balance water.

[0048] Comparative Example 9 A plant extract repair essence is composed of the following raw materials in percentage by weight: 3% glycerin, 7% butylene glycol, 0.03% disodium EDTA, 0.15% acrylates / C10-30 alkyl acrylate crosspolymer, 0.25% allantoin, 0.1% sodium hyaluronate, 0.5% panthenol, 0.12% xanthan gum, 0.08% hydroxyethyl cellulose, 0.12% methylparaben, 2% PEG / PPG-17 / 6 copolymer, 2% betaine, 0.05% caprylhydroxamic acid, 0.15% triethanolamine, 5% Centella asiatica plant extract repair agent prepared in Comparative Example 5, and the balance water.

[0049] Comparative Example 10 A plant extract repair essence is composed of the following raw materials in percentage by weight: 3% glycerin, 7% butylene glycol, 0.03% disodium EDTA, 0.15% acrylates / C10-30 alkyl acrylate crosspolymer, 0.25% allantoin, 0.1% sodium hyaluronate, 0.5% panthenol, 0.12% xanthan gum, 0.08% hydroxyethyl cellulose, 0.12% methylparaben, 2% PEG / PPG-17 / 6 copolymer, 2% betaine, 0.05% caprylhydroxamic acid, 0.15% triethanolamine, 5% Centella asiatica plant extract repair agent prepared in Comparative Example 6, and the balance water.

[0050] Volunteers were recruited to evaluate the efficacy of the plant extract repair essences prepared in Example 4 and Comparative Examples 7-10. The specific test process is as follows: (1) Screening of volunteers: 30 healthy women aged 18-50 years old, who voluntarily participated and signed the informed consent form and had not undergone other efficacy clinical tests recently.

[0051] (2) Test index: TEWL value.

[0052] (3) Evaluation method: First, divide the inner skin of the arms of the volunteers into three 3*3 cm square test areas, that is, each volunteer has six test areas, and measure the initial TEWL value of each test area. Then use 3M tape to repeatedly tear the test area 10 times to construct a skin barrier damage model. Then apply 0.1g of the plant extract repair essence prepared in Example 4 and Comparative Examples 7-10 respectively, and set up a blank control group. After the volunteers sit quietly for 30 minutes, measure the TEWL value of each test area. Calculate the average TEWL of each test group. See the specific results. Figure 1 .

[0053] Depend on Figure 1 It can be seen that the plant extract repair essence prepared in Example 4 of the present invention can effectively reduce the TEWL value of the skin barrier damage model, reflecting a good repair effect. Comparison of the test results of Example 4 with those of Comparative Examples 8-10 shows that the three ingredients of olive leaves, Centella asiatica roots, and oat kernels have a mutually reinforcing effect. Comparison of the test results of Example 4 with those of Comparative Example 7 shows that the preparation process adopted by the present invention is superior to the conventional process.

[0054] The technical features of the above embodiments may be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0055] The above embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for those of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A Centella asiatica plant extract repair agent, characterized in that: The composition includes the following ingredients: olive (OLEA EUROPAEA) leaf extract, Centella asiatica (CENTELLA ASIATICA) root extract, and oat (AVENA SATIVA) kernel extract.

2. The Centella asiatica plant extract repair agent according to claim 1, characterized in that The preparation process includes the following steps: (1) Weigh the following raw materials in parts by weight: 1-10 parts of olive leaves, 1-10 parts of Centella asiatica roots, and 1-10 parts of oat kernels, and grind and sieve to obtain a mixed powder; (2) Mix the mixed powder, water and cellulase, stir and hydrolyze; add ethanol; soak and extract under stirring, filter and obtain filtrate and residue; (3) Add an equal volume of glycerol to the filtrate, remove the ethanol by rotary evaporation, and centrifuge to obtain the supernatant; (4) drying the filter residue and extracting it with supercritical CO2 for 2-3 hours to obtain an oily extract; (5) Heat Tween 80, oily extract, lecithin, and sucrose cocoate to dissolve, and slowly drop the clear liquid into the mixture while stirring; then use an ultrasonic homogenizer to process the mixture three times, and filter the mixture with a filter membrane to obtain the Centella asiatica plant extract repair agent.

3. The Centella asiatica plant extract repair agent according to claim 2, characterized in that: The weight parts of the raw materials in step (1) are: 6 parts of olive leaves, 3 parts of Centella asiatica roots, and 7 parts of oat kernels.

4. The Centella asiatica plant extract repair agent according to claim 2, characterized in that: In step (2), the mass ratio of the mixed powder, water and cellulase is 1:5-10:0.01-0.

05.

5. The Centella asiatica plant extract repair agent according to claim 2, characterized in that: The stirring hydrolysis conditions in step (2) are: stirring hydrolysis at 50-55° C. for 3-5 hours.

6. The Centella asiatica plant extract repair agent according to claim 2, characterized in that: The volume concentration of ethanol in step (2) reaches 30-50%.

7. The Centella asiatica plant extract repair agent according to claim 2, characterized in that: The process conditions of the supercritical CO2 extraction in step (4) are: extraction temperature 55°C, extraction pressure 30MPa, and CO2 circulation volume 0.6kg / g.

8. The Centella asiatica plant extract repair agent according to claim 2, characterized in that: The mass ratio of Tween 80, oily extract, lecithin, sucrose cocoate and supernatant in step (5) is 1-5:1-10:1-5:0.1-1:50-80; the filter membrane is a 0.22 μm organic microporous filter membrane.

9. Use of the Centella asiatica plant extract repairing agent according to any one of claims 1 to 8 in the preparation of cosmetics.

10. A plant extract repair essence, composed of the following raw materials in percentage by weight: Glycerin 3%, butylene glycol 7%, disodium EDTA 0.03%, acrylates / C10-30 alkyl acrylate crosspolymer 0.15%, allantoin 0.25%, sodium hyaluronate 0.1%, panthenol 0.5%, xanthan gum 0.12%, hydroxyethyl cellulose 0.08%, methylparaben 0.12%, PEG / PPG-17 / 6 copolymer 2%, betaine 2%, caprylhydroxamic acid 0.05%, triethanolamine 0.15%, Centella asiatica plant extract repair agent according to any one of claims 1-8 1-10%, and water as the balance.

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