Formula of blood-nourishing and tendon-nourishing pill

Abstract

The invention discloses a formula of blood-nourishing and tendon-nourishing pills, which is prepared from the following components in parts by weight: 7 to 9 parts of radix angelicae sinensis, 13 to 17 parts of caulis spatholobi, 30 to 35 parts of wine-fried radix polygoni multiflori, 13 to 17 parts of radix paeoniae rubra, 13 to 17 parts of radix dipsaci and 13 to 17 parts of herba taxilli. The modified dietary fiber and hydroxymethyl cellulose are added into the formula of the blood-nourishing and tendon-nourishing pill, so that the disintegration property and the acting effect on a patient of the blood-nourishing and tendon-nourishing pill are effectively improved, the sugar content of the blood-nourishing and tendon-nourishing pill is also reduced, and the excessive influence of the blood-nourishing and tendon-nourishing pill on the blood sugar of the patient is avoided.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine preparations, and in particular to a formula of Yangxue Rongjin Pills. Background Art

[0002] Yangxue Rongjin Pills is a Chinese patent medicine with the effects of nourishing blood, relaxing muscles, dispelling wind and unblocking meridians. This medicine is mainly used to treat symptoms such as muscle and bone pain, limb numbness, muscle atrophy, joint discomfort, etc. caused by insufficient qi and blood, traumatic injuries, etc. Its ingredients mainly include Angelica sinensis, Millettia reticulata, Polygonum multiflorum, Red Peony Root, Dipsacus asper, Viscum album and other Chinese herbal medicines. It effectively relieves the above symptoms through multiple mechanisms of action such as nourishing blood and activating blood circulation, nourishing the liver and kidneys, dispelling wind and unblocking meridians, etc.

[0003] Prior art, a Chinese patent with publication number CN116139206A discloses a Yangxue Rongjin Pill and a preparation method thereof, comprising the following components in parts by weight: 10-20 parts of angelica sinensis, 20-30 parts of red peony root, 12-22 parts of clematis root, 10-20 parts of tabulaeformis, 22-33 parts of red bean, 20-30 parts of Millettia reticulata, 15-25 parts of Atractylodes macrocephala, 18-28 parts of Dipsacus asper, 20-30 parts of Lycopodiella ciliata, 10-20 parts of dried tangerine peel, 15-25 parts of psoralea corylifolia, 15-40 parts of mistletoe, 20-30 parts of radix polygonati spatholobi, 15-35 parts of codonopsis pilosula, 40-60 parts of polygonum multiflorum, 10-20 parts of costus root, 1-5 parts of disintegrant, 1-3 parts of lubricant and 450-550 parts of honey; the patent accelerates the release of the effective ingredients in Yangxue Rongjin Pills by adding disintegrants, which can greatly alleviate the patient's pain and accelerate the patient's recovery.

[0004] However, the amount of honey used in the Yangxue Rongjin Pills in the prior art is relatively large, which is not beneficial for patients who need to control blood sugar. In addition, the disintegration performance of the traditional Yangxue Rongjin Pills and the absorption and digestion performance of the Yangxue Rongjin Pills by the human body are poor, resulting in the effect of the Yangxue Rongjin Pills on patients during clinical trials needing to be further improved.

[0005] In view of the technical defects in this aspect, a solution is now proposed. Summary of the invention

[0006] The purpose of the present invention is to provide a formula of Yangxue Rongjin Pills, which is used to solve the technical problems in the prior art that the honey content in Yangxue Rongjin Pills is high and the effect of Yangxue Rongjin Pills on patients during clinical trials needs to be further improved.

[0007] The purpose of the present invention can be achieved through the following technical solutions:

[0008] A formula for nourishing blood and strengthening tendons pills comprises the following components by weight: 7-9 parts of angelica sinensis, 13-17 parts of millettia spatholobi, 30-35 parts of wine-fried polygonum multiflorum, 13-17 parts of red peony root, 13-17 parts of dipsaci, 13-17 parts of parasitic plant, 7-9 parts of wine-fried clematis radix, 13-17 parts of striata herba, 7-9 parts of scutellaria baicalensis, 7-9 parts of tabulaeformis, 10-14 parts of salt psoralea corylifolia, 13-17 parts of codonopsis pilosula, 10-14 parts of stir-fried atractylodes macrocephala, 7-9 parts of tangerine peel, 7-9 parts of costusroot, 13-17 parts of red bean, 2-4 parts of lubricant, 8-12 parts of carboxymethyl cellulose, 4-7 parts of modified dietary fiber and 30-40 parts of refined honey.

[0009] Furthermore, the preparation method of the modified dietary fiber is: adding acid detergent fiber and acetic acid solution into a reactor and stirring, adding cellulase into the reactor, raising the temperature of the reactor to 53-57° C., keeping the temperature for enzymolysis for 4-5 hours, and post-treating to obtain the modified dietary fiber.

[0010] The synthetic reaction mechanism of modified dietary fiber is:

[0011] The acetic acid solution provides a good reaction environment for the reaction system. Under the heat preservation catalytic environment, cellulase can specifically act on the β-1,4-glycosidic bonds in the cellulose molecules, converting insoluble dietary fiber into soluble dietary fiber. After ethanol precipitation, modified dietary fiber is prepared.

[0012] Furthermore, the dosage ratio of the acidic detergent fiber and the citric acid solution is 1g:12-15mL, the acetic acid solution is composed of 1wt% acetic acid and sodium acetate, the pH of the acetic acid solution is 4.8-5.1, and the amount of cellulase added is 22-26U / g. The post-treatment includes: after the reaction is completed, the temperature of the reactor is increased to the reflux of the system, the heat preservation treatment is performed for 8-10min, the temperature of the reactor is lowered to room temperature, and four times the volume of anhydrous ethanol is added to the reactor at a volume ratio of 1:4, mixed evenly, allowed to stand for 5h, filtered, the filter cake is washed with anhydrous ethanol 3 times and then dried, and the filter cake is transferred to a drying oven at a temperature of 50-60°C, vacuum dried to constant weight, crushed, and passed through a 100-mesh sieve to obtain modified dietary fiber.

[0013] Further, the acid detergent fiber is processed by the following steps:

[0014] A1. Add sweet potato residue and purified water into a reactor and stir, adjust the pH of the reaction system to 6-6.5, increase the temperature of the reactor to 50-60° C., add amylase to the reactor, keep the temperature for 60-80 min, inactivate the enzyme, and obtain an amylase hydrolyzate;

[0015] A2. Adjust the pH of the amylase hydrolyzate to 3.8-4.2, add saccharifying enzyme to the reactor containing the amylase hydrolyzate, increase the temperature of the reactor to 60-70°C, keep the temperature for enzymolysis for 50-60 minutes, and post-treat to obtain acid detergent fiber.

[0016] The synthetic reaction mechanism of acid detergent fiber is:

[0017] Sweet potato residue is rich in starch, cellulose, hemicellulose, lignin, protein and other substances. Amylase specifically acts on the starch in the sweet potato residue to decompose the starch into glucose molecules or smaller glucose oligosaccharides to form an amylase hydrolyzate. Under acidic conditions, saccharifying enzymes will continue to hydrolyze glucose oligosaccharides into single glucose molecules, and then ethanol is used to precipitate the cellulose, hemicellulose, lignin and the like in the solution from the acidic liquid in the form of precipitation to prepare acidic detergent fiber.

[0018] Furthermore, in step A1, the dosage ratio of the sweet potato residue and purified water is 1g:12-15mL, the amylase is 1,4-α-D-glucan glucanohydrolase, the addition amount of amylase is 300-360U / g, and the method of inactivating the enzyme is: after the enzymatic hydrolysis is completed, the temperature of the reactor is increased to the reflux of the system, and the reflux is maintained for 8-10 minutes to obtain an amylase hydrolyzate.

[0019] Furthermore, in step A2, the saccharifying enzyme is α-1,4-glucose hydrolase, and the added amount of the saccharifying enzyme is 230-250U / g. The post-treatment includes: after the reaction is completed, the temperature of the reactor is increased to the reflux of the system, and the reflux is kept warm for 8-10 minutes, the temperature of the reactor is lowered to room temperature, filtered, the filtrate and ethanol are mixed evenly in a volume ratio of 1:4, allowed to stand for 5 hours, filtered, the filter cake is washed with anhydrous ethanol 3 times and then dried, the filter cake is transferred to a drying oven at a temperature of 50-60°C, vacuum dried to constant weight, and crushed to obtain acid detergent fiber.

[0020] Furthermore, the preparation method of the wine-roasted wire clematis is as follows: slice the wire clematis and mix it evenly with rice wine in a ratio of 10g:2.5-3.5mL, seal and steam for 2.5-3.5h in a temperature environment of 45-50℃, take it out, put it in a medicine stir-frying machine at a temperature of 80-90℃ and stir-fry for 40-50min, discharge it, cool it, transfer it to a drying oven at a temperature of 50-60℃, and vacuum dry it to constant weight to obtain the wine-roasted wire clematis.

[0021] Furthermore, the preparation method of wine-roasted Polygonum multiflorum is as follows: soybean powder and rice wine are evenly mixed at a ratio of 1 g:10 mL, sealed and moistened for 6-8 hours at a temperature of 50-60°C, cooled to room temperature, filtered, and the rice wine filtrate is obtained; sliced ​​Polygonum multiflorum is evenly mixed with the rice wine filtrate at a ratio of 10 g:2-3 mL, sealed and moistened for 3-5 hours at a temperature of 45-50°C, taken out, placed in a medicine stir-frying machine at a temperature of 80-90°C and stir-fried for 40-50 minutes, discharged, cooled, transferred to a drying oven at a temperature of 50-60°C, and vacuum dried to constant weight to obtain wine-roasted Polygonum multiflorum.

[0022] The present invention also provides a method for preparing Yangxue Rongjin Pills, comprising the following steps:

[0023] S1. According to the formula, angelica sinensis, Millettia reticulata, wine-fried Polygonum multiflorum, red peony root, Dipsacus asper, Morus alba, wine-fried Clematis chinensis, Herba Lycopodii, Herba Polygoni Multiflori, Pinellia ternata, Psoralea corylifolia, Codonopsis pilosula, stir-fried Atractylodes macrocephala, dried orange peel, Aucklandia lappa, Adzuki bean, lubricant, carboxymethyl cellulose and modified dietary fiber were ground into powder, mixed and passed through a 100-mesh sieve to obtain a mixed powder;

[0024] S2, adding honey into a honey refining pot at a temperature of 50-60° C., and refining the honey until the water content of the honey is 18-20%, thereby obtaining refined honey;

[0025] S3, after the mixed powder and the refined honey are evenly mixed, pilling is performed to obtain a Yangxue Rongjin Pill blank;

[0026] S4. Place the Yangxue Rongjin Pills blank in a drying oven and dry it to a moisture content of 6-10% to obtain Yangxue Rongjin Pills.

[0027] The present invention has the following beneficial effects:

[0028] 1. The formula of the Yangxue Rongjin Pills of the present invention is optimized by optimizing the formula of the existing Yangxue Rongjin Pills, exploring the dosage ratio of each component, and roasting the Polygonum multiflorum and Clematis chinensis with wine, thereby further improving the effect of the Yangxue Rongjin Pills. Hydroxymethylcellulose is coordinated with modified dietary fiber and refined honey, and hydroxymethylcellulose and refined honey are coordinated with each other. While reducing the usage of refined honey, the coordination with the modified dietary fiber also improves the disintegration of the Yangxue Rongjin Pills, making the Yangxue Rongjin Pills more friendly to patients with blood sugar control requirements, and its good disintegration is more conducive to the absorption of the Yangxue Rongjin Pills in the patient's body, thereby improving the efficacy of the Yangxue Rongjin Pills.

[0029] 2. The formula of the Yangxue Rongjin Pills of the present invention increases the hydrophilicity of the modified dietary fiber, so that the soluble dietary fiber has strong water absorption and swelling properties, and can absorb a large amount of water in the body and swell to form a gel-like substance. The hydroxymethyl cellulose has good viscosity and film-forming properties, so that the Yangxue Rongjin Pills can quickly disintegrate into a gel after entering the human body, and use it as bait for beneficial bacteria in the intestine, thereby promoting the reproduction of beneficial bacteria, further promoting the decomposition and absorption of the medicinal ingredients of the Yangxue Rongjin Pills, and improving the medicinal efficacy of the Yangxue Rongjin Pills. The thickening and film-forming properties of the modified dietary fiber and hydroxymethyl cellulose cooperate with each other to act together on refined honey, constructing a sticky system in the formula of the Yangxue Rongjin Pills, while ensuring good pilling, reducing the amount of honey used, thereby reducing the sugar content in the Yangxue Rongjin Pills, and reducing the effect of the Yangxue Rongjin Pills on the patient's blood sugar.

[0030] 3. The formula of the Yangxue Rongjin Pill of the present invention: the iron wire clematis has the effects of dispelling wind and dampness, unblocking meridians and relieving pain, and the yellow wine can help yang and dispel cold, promote the circulation of qi and blood. After being roasted with wine, the effect of dispelling wind and dampness is enhanced, and the patient's tendon discomfort caused by rheumatic arthralgia is improved. Soybean powder is rich in various nutrients. After being moistened with yellow wine, the effective ingredients in soybeans are extracted. Polygonum multiflorum itself has the effects of nourishing liver and kidney, nourishing blood and invigorating essence, and its medicinal ingredients include lecithin, chrysophanol, rhubarb and the like. The alcohol in the yellow wine and the soybean extract can chemically react with the acids and other active substances in the Polygonum multiflorum material to generate compounds with stronger biological activity or that are easier to be absorbed and utilized by the human body, thereby enhancing the medicinal effect of Polygonum multiflorum. The wine-roasted Polygonum multiflorum and the wine-roasted iron wire clematis cooperate with each other, so that the medicinal effect of the Yangxue Rongjin Pill is further dispersed and enhanced, and the effect of the Yangxue Rongjin Pill on patients during the clinical trial is improved. DETAILED DESCRIPTION

[0031] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] Example 1

[0033] A method for preparing a modified dietary fiber of the present embodiment comprises the following steps:

[0034] S1. Enzymatic hydrolysis of sweet potato residue

[0035] Sweet potato residue and purified water were added to the reactor at a ratio of 1 g:12 mL and stirred. The pH of the reaction system was adjusted to 6. The temperature of the reactor was increased to 50°C. 1,4-α-D-glucan glucanohydrolase was added to the reactor at an addition amount of 300 U / g. The enzymatic hydrolysis was kept warm for 60 minutes. The temperature of the reactor was increased to reflux of the system, and the system was kept warm for 8 minutes to obtain an amylase hydrolyzate.

[0036] S2. Preparation of acid detergent fiber

[0037] The pH of the amylase hydrolyzate was adjusted to 3.8, and α-1,4-glucose hydrolase was added to the reactor containing the amylase hydrolyzate at an amount of 230 U / g. The temperature of the reactor was increased to 60°C, and the enzymatic hydrolysis was kept warm for 50 minutes. The temperature of the reactor was increased to reflux of the system, and the reflux was kept warm for 8 minutes. The temperature of the reactor was lowered to room temperature, and the filtrate was filtered. The filtrate was mixed with ethanol in a volume ratio of 1:4, and allowed to stand for 5 hours. The filter cake was washed with anhydrous ethanol for 3 times and then dried. The filter cake was transferred to a drying oven at a temperature of 50°C, vacuum dried to constant weight, and crushed to obtain acid detergent fiber.

[0038] S3. Preparation of modified dietary fiber

[0039] Adding sodium acetate to a 1 wt % acetic acid solution to adjust the pH of the system to 4.8 to obtain an acetic acid solution;

[0040] Acidic detergent fiber and acetic acid solution were added to the reactor at a ratio of 1 g:12 mL and stirred. Cellulase was added to the reactor at an amount of 22 U / g. The temperature of the reactor was raised to 53°C and the enzymolysis was kept warm for 4 hours. The temperature of the reactor was raised to reflux and kept warm for 8 minutes. The temperature of the reactor was lowered to room temperature. Four times the volume of anhydrous ethanol was added to the reactor at a volume ratio of 1:4. The mixture was mixed evenly and allowed to stand for 5 hours. The filter cake was washed with anhydrous ethanol for 3 times and then dried. The filter cake was transferred to a drying oven at a temperature of 50°C, vacuum dried to constant weight, crushed, and passed through a 100-mesh sieve to obtain modified dietary fiber.

[0041] Example 2

[0042] A method for preparing a modified dietary fiber of the present embodiment comprises the following steps:

[0043] S1. Enzymatic hydrolysis of sweet potato residue

[0044] Sweet potato residue and purified water were added to the reactor at a ratio of 1 g:14 mL and stirred. The pH of the reaction system was adjusted to 6.3. The temperature of the reactor was increased to 55°C. 1,4-α-D-glucan glucanohydrolase was added to the reactor at an addition amount of 330 U / g. The enzymatic hydrolysis was kept warm for 70 minutes. The temperature of the reactor was increased to reflux of the system and kept warm for 9 minutes to obtain an amylase hydrolyzate.

[0045] S2. Preparation of acid detergent fiber

[0046] The pH of the amylase hydrolyzate was adjusted to 4.0, and α-1,4-glucose hydrolase was added to the reactor containing the amylase hydrolyzate at an amount of 240 U / g. The temperature of the reactor was increased to 65°C, and the enzymatic hydrolysis was kept warm for 55 minutes. The temperature of the reactor was increased to reflux of the system, and the reflux was kept warm for 9 minutes. The temperature of the reactor was lowered to room temperature, filtered, and the filtrate was mixed with ethanol in a volume ratio of 1:4, allowed to stand for 5 hours, filtered, the filter cake was washed with anhydrous ethanol for 3 times and then dried, and the filter cake was transferred to a drying oven at a temperature of 55°C, vacuum dried to constant weight, and crushed to obtain acid detergent fiber.

[0047] S3. Preparation of modified dietary fiber

[0048] Adding sodium acetate to a 1 wt % acetic acid solution to adjust the pH of the system to 5.0 to obtain an acetic acid solution;

[0049] Acidic detergent fiber and acetic acid solution were added to the reactor at a ratio of 1 g:14 mL and stirred. Cellulase was added to the reactor at an amount of 24 U / g. The temperature of the reactor was raised to 55°C and the enzymolysis was kept warm for 4.5 hours. The temperature of the reactor was raised to reflux and kept warm for 9 minutes. The temperature of the reactor was lowered to room temperature. Four times the volume of anhydrous ethanol was added to the reactor at a volume ratio of 1:4. The mixture was mixed evenly and allowed to stand for 5 hours. The filter cake was filtered and washed with anhydrous ethanol for 3 times and then dried. The filter cake was transferred to a drying oven at a temperature of 55°C, vacuum dried to constant weight, crushed, and passed through a 100-mesh sieve to obtain modified dietary fiber.

[0050] Example 3

[0051] A method for preparing a modified dietary fiber of the present embodiment comprises the following steps:

[0052] S1. Enzymatic hydrolysis of sweet potato residue

[0053] Sweet potato residue and purified water were added to the reactor at a ratio of 1 g:15 mL and stirred. The pH of the reaction system was adjusted to 6.5. The temperature of the reactor was increased to 60°C. 1,4-α-D-glucan glucanohydrolase was added to the reactor at an addition amount of 360 U / g. The enzymatic hydrolysis was kept warm for 80 minutes. The temperature of the reactor was increased to reflux of the system and kept warm for 10 minutes to obtain an amylase hydrolyzate.

[0054] S2. Preparation of acid detergent fiber

[0055] The pH of the amylase hydrolyzate was adjusted to 4.2, and α-1,4-glucose hydrolase was added to the reactor containing the amylase hydrolyzate at an amount of 250 U / g. The temperature of the reactor was increased to 70°C, and the enzymatic hydrolysis was kept warm for 60 minutes. The temperature of the reactor was increased to reflux of the system, and the reflux was kept warm for 10 minutes. The temperature of the reactor was lowered to room temperature, and the filtrate was filtered, and the filtrate was mixed with ethanol in a volume ratio of 1:4, and allowed to stand for 5 hours. The filter cake was washed with anhydrous ethanol for 3 times and then dried. The filter cake was transferred to a drying oven at a temperature of 60°C, vacuum dried to constant weight, and crushed to obtain acid detergent fiber.

[0056] S3. Preparation of modified dietary fiber

[0057] Adding sodium acetate to a 1 wt % acetic acid solution to adjust the pH of the system to 5.1 to obtain an acetic acid solution;

[0058] Acidic detergent fiber and acetic acid solution were added to the reactor at a ratio of 1 g:15 mL and stirred. Cellulase was added to the reactor at an amount of 26 U / g. The temperature of the reactor was raised to 57°C and the enzymolysis was kept warm for 5 hours. The temperature of the reactor was raised to reflux and kept warm for 10 minutes. The temperature of the reactor was lowered to room temperature. Four times the volume of anhydrous ethanol was added to the reactor at a volume ratio of 1:4. The mixture was mixed evenly and allowed to stand for 5 hours. The filter cake was washed with anhydrous ethanol for 3 times and then dried. The filter cake was transferred to a drying oven at a temperature of 60°C, vacuum dried to constant weight, crushed, and passed through a 100-mesh sieve to obtain modified dietary fiber.

[0059] Example 4

[0060] This embodiment provides a method for preparing wine-roasted Clematis chinensis, comprising the following steps:

[0061] Slice the wire Clematis chinensis and mix it evenly with rice wine in a ratio of 10g:2.5mL. Keep it in a sealed container at 45℃ for 2.5h. Take it out and stir-fry it in a medicine stir-fryer at 80℃ for 40min. Discharge it, let it cool, transfer it to a drying oven at 50℃, and vacuum dry it to constant weight to obtain the wine-roasted wire Clematis chinensis.

[0062] Example 5

[0063] This embodiment provides a method for preparing wine-roasted Clematis chinensis, comprising the following steps:

[0064] Slice the wire Clematis chinensis and mix it evenly with rice wine in a ratio of 10g:3.0mL. Keep it in a sealed container at 47℃ for 3h, take it out, and stir-fry it in a medicine stir-fryer at 85℃ for 45min. Discharge the material, let it cool, transfer it to a drying oven at 55℃, and vacuum dry it to constant weight to obtain the wine-roasted wire Clematis chinensis.

[0065] Example 6

[0066] This embodiment provides a method for preparing wine-roasted Clematis chinensis, comprising the following steps:

[0067] Slice the wire Clematis chinensis and mix it evenly with rice wine in a ratio of 10g:3.5mL. Keep it in a sealed container at 50℃ for 3.5h. Take it out and stir-fry it in a medicine stir-fryer at 90℃ for 50min. Discharge it, let it cool, transfer it to a drying oven at 60℃, and vacuum dry it to constant weight to obtain the wine-roasted wire Clematis chinensis.

[0068] Example 7

[0069] This embodiment provides a method for preparing wine-fried Polygonum multiflorum, comprising the following steps:

[0070] Mix soybean powder and rice wine at a ratio of 1g:10mL, seal and moisten for 6 hours at 50℃, cool to room temperature, filter and obtain rice wine filtrate; slice Polygonum multiflorum and mix with rice wine filtrate at a ratio of 10g:2mL, seal and moisten for 3 hours at 45℃, take out, stir-fry in a medicine stir-fryer at 80℃ for 40min, discharge, cool, transfer to a drying oven at 50℃, and vacuum dry to constant weight to obtain wine-roasted Polygonum multiflorum.

[0071] Example 8

[0072] This embodiment provides a method for preparing wine-fried Polygonum multiflorum, comprising the following steps:

[0073] Mix soybean powder and rice wine at a ratio of 1g:10mL, seal and moisten for 7h at 55℃, cool to room temperature, filter and obtain rice wine filtrate; slice Polygonum multiflorum and mix with rice wine filtrate at a ratio of 10g:2.5mL, seal and moisten for 4h at 47℃, take out, stir-fry in a medicine stirrer at 85℃ for 45min, discharge, cool, transfer to a drying oven at 55℃, and vacuum dry to constant weight to obtain wine-roasted Polygonum multiflorum.

[0074] Example 9

[0075] This embodiment provides a method for preparing wine-fried Polygonum multiflorum, comprising the following steps:

[0076] Mix soybean powder and rice wine at a ratio of 1g:10mL, seal and moisten for 8h at 60℃, cool to room temperature, filter and obtain rice wine filtrate; slice Polygonum multiflorum and mix with rice wine filtrate at a ratio of 10g:3mL, seal and moisten for 5h at 50℃, take out, stir-fry in a medicine stir-fryer at 90℃ for 50min, discharge, cool, transfer to a drying oven at 60℃, and vacuum dry to constant weight to obtain wine-roasted Polygonum multiflorum.

[0077] Example 10

[0078] This embodiment provides a method for preparing Yangxue Rongjin Pills, comprising the following steps:

[0079] Step 1: Prepare refined honey

[0080] Add fresh honey into a honey refining pot at a temperature of 50°C and refine it until the moisture content of the honey is 18% to obtain refined honey.

[0081] Step 2: Prepare the ingredients

[0082] Weigh by weight: 7 parts of Angelica sinensis, 13 parts of Millettia spatholobi, 30 parts of wine-fried Polygonum multiflorum prepared in Example 7, 13 parts of red peony root, 13 parts of Dipsacus asper, 13 parts of Viscum album, 7 parts of wine-fried Clematis chinensis prepared in Example 4, 13 parts of Lycopodiella ciliata, 7 parts of Radix schizonepetae, 7 parts of Pinus tabulaeformis, 10 parts of Psoralea corylifolia, 13 parts of Codonopsis pilosula, 10 parts of stir-fried Atractylodes macrocephala, 7 parts of dried tangerine peel, 7 parts of costus root, 13 parts of red bean, 2 parts of sodium stearate, 8 parts of carboxymethyl cellulose, 4 parts of modified dietary fiber prepared in Example 1, and 30 parts of refined honey.

[0083] Step 3: Prepare mixed powder

[0084] Grind angelica, Millettia reticulata, wine-fried Polygonum multiflorum, red peony root, Dipsacus asper, Viscum album, wine-fried Clematis chinensis, Herba Lycopodii, Herba Polygoni Multiflori, Thunbergiae lappa, Psoralea corylifolia, Codonopsis pilosula, stir-fried Atractylodes macrocephala, dried orange peel, Aucklandia lappa, Adzuki bean, sodium stearate, carboxymethyl cellulose and the modified dietary fiber prepared in Example 1 into powder, mix and pass through a 100-mesh sieve to obtain a mixed powder.

[0085] Step 4: Prepare Yangxue Rongjin Pills

[0086] After the mixed powder and refined honey are evenly mixed, 60 parts by weight of purified water are added, and after being evenly mixed, the medicine ball is placed on a ball drying table, kneaded into balls of uniform size, cooled to room temperature and then dried, and the ball strips are broken into small pieces and pelleted by a pill making machine to obtain the Yangxue Rongjin Pill blank;

[0087] The Yangxue Rongjin Pills blank is placed in a drying oven and dried to a moisture content of 6% to obtain Yangxue Rongjin Pills.

[0088] Embodiment 11

[0089] This embodiment provides a method for preparing Yangxue Rongjin Pills, comprising the following steps:

[0090] Step 1: Prepare refined honey

[0091] Add fresh honey into a honey refining pot at a temperature of 55°C and refine it until the moisture content of the honey is 19% to obtain refined honey.

[0092] Step 2: Prepare the ingredients

[0093] Weigh by weight: 8 parts of Angelica sinensis, 15 parts of Millettia spatholobi, 33 parts of wine-fried Polygonum multiflorum prepared in Example 8, 15 parts of red peony root, 15 parts of Dipsacus asper, 15 parts of Viscum album, 8 parts of wine-fried Clematis chinensis prepared in Example 5, 15 parts of Herba Lycopodii, 8 parts of Herba Polygoni Multiflori, 8 parts of Pinus tabulaeformis, 12 parts of Psoralea corylifolia, 15 parts of Codonopsis pilosula, 12 parts of stir-fried Atractylodes macrocephala, 8 parts of dried tangerine peel, 8 parts of costus root, 15 parts of adzuki bean, 3 parts of calcium stearate, 10 parts of carboxymethyl cellulose, 5.5 parts of modified dietary fiber in Example 2, and 35 parts of refined honey.

[0094] Step 3: Prepare mixed powder

[0095] Grind angelica, Millettia reticulata, wine-fried Polygonum multiflorum prepared in Example 8, red peony root, Dipsacus asper, Viscum album, wine-fried Clematis chinensis prepared in Example 5, Lycopodiella ciliata, Herba Polygoni Multiflori, Pinus tabulaeformis, Psoralea corylifolia, Codonopsis pilosula, stir-fried Atractylodes macrocephala, dried orange peel, costusroot, adzuki bean, calcium stearate, carboxymethyl cellulose and the modified dietary fiber prepared in Example 2 into powder, mix and pass through a 100-mesh sieve to obtain a mixed powder.

[0096] Step 4: Prepare Yangxue Rongjin Pills

[0097] After the mixed powder and refined honey are evenly mixed, 65 parts by weight of purified water are added, and after evenly mixed, the medicine ball is placed on a ball drying table, kneaded into balls of uniform size, cooled to room temperature and then dried, and the ball strips are broken into small pieces and pelleted by a pill making machine to obtain the Yangxue Rongjin Pill blank;

[0098] The Yangxue Rongjin Pills blank is placed in a drying oven and dried to a moisture content of 8% to obtain Yangxue Rongjin Pills.

[0099] Example 12

[0100] This embodiment provides a method for preparing Yangxue Rongjin Pills, comprising the following steps:

[0101] Step 1: Prepare refined honey

[0102] Add fresh honey into a honey refining pot at a temperature of 60°C and refine it until the moisture content of the honey is 20% to obtain refined honey.

[0103] Step 2: Prepare the ingredients

[0104] Weigh by weight: 9 parts of Angelica sinensis, 17 parts of Millettia spatholobi, 35 parts of wine-fried Polygonum multiflorum prepared in Example 9, 17 parts of red peony root, 17 parts of Dipsacus asper, 17 parts of Viscum album, 9 parts of wine-fried Clematis chinensis prepared in Example 6, 17 parts of Lycopodiella ciliata, 9 parts of Herba Polygoni Multiflori, 9 parts of Pinus tabulaeformis, 14 parts of Psoralea corylifolia, 17 parts of Codonopsis pilosula, 14 parts of stir-fried Atractylodes macrocephala, 9 parts of dried tangerine peel, 9 parts of costusroot, 17 parts of adzuki bean, 4 parts of zinc stearate, 12 parts of carboxymethyl cellulose, 7 parts of modified dietary fiber prepared in Example 3, and 40 parts of refined honey.

[0105] Step 3: Prepare mixed powder

[0106] Grind angelica, Millettia reticulata, wine-fried Polygonum multiflorum prepared in Example 9, red peony root, Dipsacus asper, Viscum album, wine-fried Clematis chinensis prepared in Example 6, Lycopodiella ciliata, Herba Polygoni Multiflori, Pinus tabulaeformis, Psoralea corylifolia, Codonopsis pilosula, stir-fried Atractylodes macrocephala, dried orange peel, costusroot, adzuki bean, zinc stearate, carboxymethyl cellulose and the modified dietary fiber prepared in Example 3 into powder, mix and pass through a 100-mesh sieve to obtain a mixed powder.

[0107] Step 4: Prepare Yangxue Rongjin Pills

[0108] After the mixed powder and refined honey are evenly mixed, 70 parts by weight of purified water are added, and after evenly mixed, the medicine ball is placed on a ball drying table, kneaded into balls of uniform size, cooled to room temperature and then dried, and the ball strips are broken into small pieces and pelleted by a pill making machine to obtain the Yangxue Rongjin Pill blank;

[0109] The Yangxue Rongjin Pills blank is placed in a drying oven and dried to a moisture content of 10% to obtain Yangxue Rongjin Pills.

[0110] Comparative Example 1

[0111] The difference between this comparative example and Example 12 is that the dietary fiber used is replaced by an equal amount of the acid detergent fiber prepared in step S2 of Example 3.

[0112] Comparative Example 2

[0113] The difference between this comparative example and Example 12 is that the iron wire clematis in Example 6 is used to replace the wine-roasted iron wire clematis, and the same amount of Polygonum multiflorum in Example 9 is used to replace the wine-roasted Polygonum multiflorum.

[0114] Comparative Example 3

[0115] The difference between this comparative example and Example 12 is that the weight of refined honey in step 2 is increased to 60 parts, and in step 3, carboxymethyl cellulose and modified dietary fiber are not added.

[0116] Performance Test:

[0117] The samples of Yangxue Rongjin Pills prepared in Comparative Examples 1-3 and Examples 9-12 were mixed with 37°C drinking water at a ratio of 1 g: 200 mL and allowed to stand. The morphology of the Yangxue Rongjin Pills was observed and the time it took to be completely dispersed in water was recorded as the disintegration time.

[0118] Clinical trials were conducted on the Yangxue Rongjin Pills prepared in Comparative Examples 1-3 and Examples 9-12. The specific experiments were as follows: Several patients with traumatic injuries were randomly selected, aged between 25 and 50 years old. The criteria for selecting patients were based on the diagnostic criteria for tissue injury in the "Guidelines for Clinical Research of New Chinese Medicines". The patients were randomly divided into 7 groups with 30 people in each group, 6 of which took the Yangxue Rongjin Pills prepared in the comparative examples and the examples, and the other group was used as the control group. The control group took sugar pills as a placebo, and each person took it twice a day, 60 pills at a time, for 2 weeks, and went to the doctor after 2 weeks. The symptom relief before and after treatment was evaluated and divided into the following 4 levels according to the different symptom manifestations before and after treatment: cured: pain, swelling, ecchymosis and other symptoms were significantly reduced, and joint movement was normal; markedly effective: pain, swelling, ecchymosis and other symptoms were reduced, and joint movement was not restricted; effective: pain, swelling, ecchymosis and other symptoms and signs were slightly reduced, and joint movement was improved; ineffective: pain, swelling, ecchymosis and other symptoms and signs were not significantly reduced, and joint movement did not change. Each patient was rated according to his or her treatment situation, and the treatment situation of all patients was counted. The specific test results are shown in Table 1 below.

[0119] Table 1-Performance test data of the sample

[0120]

[0121] Data Analysis:

[0122] A comparative analysis of the data in Table 1 above shows that the disintegration time of the Yangxue Rongjin Pills prepared by the present invention reaches 20.5 min, the probability of clinical recovery for patients with traumatic injuries reaches 60.0%, the probability of ineffectiveness is reduced to 10.0%, the total effective rate reaches 90%, and all performance tests are better than the control examples, indicating that the present invention optimizes the traditional Yangxue Rongjin Pills formula, and adds modified dietary fiber and hydroxymethyl cellulose to the formula of the Yangxue Rongjin Pills, which not only effectively improves the disintegration of the Yangxue Rongjin Pills and the effect on patients, but also reduces the sugar content of the Yangxue Rongjin Pills, thereby avoiding excessive influence of the Yangxue Rongjin Pills on patients' blood sugar.

[0123] The above contents are merely examples and explanations of the structure of the present invention. The technicians in this technical field may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the structure of the invention or exceed the scope defined by the claims, they should all fall within the protection scope of the present invention.

Claims

1. A formula of Yangxue Rongjin Pills, characterized in that: The invention comprises the following components in parts by weight: 7-9 parts of angelica sinensis, 13-17 parts of millettia spatholobi, 30-35 parts of wine-fried polygonum multiflorum, 13-17 parts of red peony root, 13-17 parts of dipsaci, 13-17 parts of parasitic plant, 7-9 parts of wine-fried clematis radix, 13-17 parts of striata herba, 7-9 parts of scutellaria baicalensis, 7-9 parts of tabulaeformis, 10-14 parts of salt psoralea corylifolia, 13-17 parts of codonopsis pilosula, 10-14 parts of stir-fried atractylodes macrocephala, 7-9 parts of tangerine peel, 7-9 parts of costusroot, 13-17 parts of adzuki bean, 2-4 parts of lubricant, 8-12 parts of carboxymethyl cellulose, 4-7 parts of modified dietary fiber and 30-40 parts of refined honey.

2. The formula of the Yangxue Rongjin Pill according to claim 1, characterized in that: The preparation method of the modified dietary fiber is as follows: acid detergent fiber and acetic acid solution are added into a reactor and stirred, cellulase is added into the reactor, the temperature of the reactor is increased to 53-57° C., the reactor is kept warm for enzymolysis for 4-5 hours, and the modified dietary fiber is obtained by post-processing.

3. The formula of a Yangxue Rongjin Pill according to claim 2, characterized in that: The dosage ratio of the acidic detergent fiber and the citric acid solution is 1g:12-15mL, the acetic acid solution is composed of 1wt% acetic acid and sodium acetate, the pH of the acetic acid solution is 4.8-5.1, and the amount of cellulase added is 22-26U / g. The post-treatment comprises: after the reaction is completed, the temperature of the reactor is increased to the reflux of the system, the heat preservation treatment is performed for 8-10min, the temperature of the reactor is reduced to room temperature, four times the volume of anhydrous ethanol is added to the reactor at a volume ratio of 1:4, mixed evenly, allowed to stand for 5h, filtered, the filter cake is washed with anhydrous ethanol for 3 times and then dried, the filter cake is transferred to a drying oven with a temperature of 50-60°C, vacuum dried to constant weight, crushed, and passed through a 100-mesh sieve to obtain modified dietary fiber.

4. The formula of the Yangxue Rongjin Pill according to claim 1, characterized in that: Acid detergent fiber is processed by the following steps: A1. Add sweet potato residue and purified water into a reactor and stir, adjust the pH of the reaction system to 6-6.5, increase the temperature of the reactor to 50-60° C., add amylase to the reactor, keep the temperature for 60-80 min, inactivate the enzyme, and obtain an amylase hydrolyzate; A2. Adjust the pH of the amylase hydrolyzate to 3.8-4.2, add saccharifying enzyme to the reactor containing the amylase hydrolyzate, increase the temperature of the reactor to 60-70°C, keep the temperature for enzymolysis for 50-60 minutes, and post-treat to obtain acid detergent fiber.

5. The formula of Yangxue Rongjin Pill according to claim 4, characterized in that: In step A1, the dosage ratio of the sweet potato residue and purified water is 1g:12-15mL, the amylase is 1,4-α-D-glucan glucanohydrolase, the addition amount of amylase is 300-360U / g, and the method of inactivating the enzyme is: after the enzymatic hydrolysis is completed, the temperature of the reactor is increased to the reflux of the system, and the reflux is kept warm for 8-10min to obtain an amylase hydrolyzate.

6. The formula of Yangxue Rongjin Pill according to claim 4, characterized in that: In step A2, the saccharifying enzyme is α-1,4-glucose hydrolase, and the added amount of the saccharifying enzyme is 230-250U / g. The post-treatment includes: after the reaction is completed, the temperature of the reactor is increased to the reflux of the system, and the reflux is kept warm for 8-10 minutes, the temperature of the reactor is lowered to room temperature, filtered, the filtrate and ethanol are mixed evenly in a volume ratio of 1:4, and allowed to stand for 5 hours, filtered, the filter cake is washed with anhydrous ethanol for 3 times and then dried, and the filter cake is transferred to a drying oven at a temperature of 50-60°C, vacuum dried to constant weight, and crushed to obtain acid detergent fiber.

7. The formula of Yangxue Rongjin Pill according to claim 1, characterized in that: The preparation method of wine-roasted wire clematis is as follows: slice the wire clematis and mix it evenly with rice wine in a dosage ratio of 10g:2.5-3.5mL, seal and steam for 2.5-3.5h in an environment at a temperature of 45-50℃, take it out, put it in a medicine stir-frying machine at a temperature of 80-90℃ and stir-fry it for 40-50min, take it out, cool it, transfer it to a drying oven at a temperature of 50-60℃, and vacuum dry it to constant weight to obtain the wine-roasted wire clematis.

8. The formula of Yangxue Rongjin Pill according to claim 1, characterized in that: The preparation method of wine-fried Polygonum multiflorum is as follows: soybean powder and rice wine are mixed evenly at a ratio of 1 g:10 mL, sealed and moistened for 6-8 hours at a temperature of 50-60°C, cooled to room temperature, filtered, and the rice wine filtrate is obtained; sliced ​​Polygonum multiflorum and mixed evenly with the rice wine filtrate at a ratio of 10 g:2-3 mL, sealed and moistened for 3-5 hours at a temperature of 45-50°C, taken out, placed in a medicine stir-frying machine at a temperature of 80-90°C and stir-fried for 40-50 minutes, discharged, cooled, transferred to a drying oven at a temperature of 50-60°C, vacuum dried to constant weight, and the wine-fried Polygonum multiflorum is obtained.

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