Method for preparing xylose and arabinose from wood fiber biomass

Abstract

The invention discloses a method for preparing xylose and arabinose by utilizing wood fiber biomass, which comprises the following steps: adding wood fiber biomass powder into an ultra-low acid aqueous solution for hydrolysis, and then carrying out solid-liquid separation to remove solid residues, thereby obtaining an aqueous solution containing xylose and arabinose. The method for preparing the xylose and the arabinose by utilizing the wood fiber biomass has the advantages of simplicity in operation, high yield of the xylose and the arabinose, low product purification cost, small corrosion to equipment, environmental friendliness and the like, and is suitable for large-scale industrial application.

Description

Technical Field

[0001] The invention relates to the technical field of biomass resource utilization, and in particular to a method for preparing xylose and arabinose by utilizing lignocellulosic biomass. Background Art

[0002] Xylose (also known as five-carbon aldose) is a component of xylan, which can activate and promote the growth of bifidobacteria (probiotics) in the human intestine, and has physiological functions such as improving the microbial environment of the human body and improving the body's immune ability. Moreover, xylose can hardly be absorbed by the human body, so it can meet the needs of diabetic people for sweet substances. In addition, xylose is the second most abundant biomass monosaccharide after glucose, and is a substrate for producing many chemicals. It can be converted into valuable biomass chemicals and fuels such as furfural, xylitol, xylulose, lactic acid, bioethanol, and bio-oil by chemical or biological methods. Arabinose (also known as arabinose, pectin sugar) is a low-calorie sugar that can selectively affect the sucrase in the small intestine, thereby inhibiting the absorption of sucrose, thereby inhibiting obesity, preventing and treating diseases related to hyperglycemia. Therefore, the development and utilization of xylose and arabinose is very important.

[0003] Lignocellulosic biomass is the most abundant renewable carbon resource in nature and is an ideal raw material for the preparation of xylose and arabinose. At present, the most commonly used method for preparing xylose is the acid method, in which the hydrogen bonds between hemicellulose and cellulose are broken in the presence of acid, and hemicellulose is hydrolyzed to produce xylose. However, the acid concentration used in the existing acid method is too high, which not only causes the xylose generated by the degradation of hemicellulose to be further converted into formic acid, acetic acid, furfural and other substances, reducing the yield of xylose, but also corrodes the production equipment, making it difficult to fully meet the requirements of practical applications.

[0004] Therefore, it is of great significance to develop a method for preparing xylose and arabinose from lignocellulosic biomass, which is simple to operate, has a high yield of xylose and arabinose, is less corrosive to equipment, and is environmentally friendly. Summary of the invention

[0005] The object of the present invention is to provide a method for preparing xylose and arabinose by utilizing lignocellulosic biomass.

[0006] The technical solution adopted by the present invention is:

[0007] A method for preparing xylose and arabinose from lignocellulosic biomass comprises the following steps: adding lignocellulosic biomass powder into an ultra-low acid aqueous solution for hydrolysis, and then performing solid-liquid separation to remove solid residues to obtain an aqueous solution containing xylose and arabinose.

[0008] Preferably, the wood fiber biomass powder is at least one of wheat straw powder, rice straw powder, corn straw powder, corn cob powder, bagasse, and wood powder.

[0009] Preferably, the mesh size of the wood fiber biomass powder is 20-120 meshes.

[0010] Preferably, the solute in the ultra-low acid aqueous solution is at least one of formic acid, p-toluenesulfonic acid, oxalic acid, acetic acid, L-malic acid, malonic acid, mandelic acid, tartaric acid, caffeic acid, sulfuric acid, and phosphoric acid.

[0011] Preferably, the concentration of the ultra-low acid aqueous solution is 0.25 g / L to 1 g / L.

[0012] Preferably, the usage ratio of the lignocellulosic biomass powder and the ultra-low acid aqueous solution is 1 g: 10 mL to 50 mL.

[0013] Preferably, the hydrolysis is carried out at a temperature of 120°C to 180°C.

[0014] Preferably, the hydrolysis time is 30 min to 240 min.

[0015] Preferably, the hydrolysis is carried out in a hydrothermal reactor.

[0016] Preferably, the solid-liquid separation is carried out by filtration.

[0017] The beneficial effects of the present invention are as follows: the method for preparing xylose and arabinose from wood fiber biomass of the present invention has the advantages of simple operation, high yield of xylose and arabinose, low product purification cost, low corrosion to equipment, and environmental friendliness, and is suitable for large-scale industrial application.

[0018] Specifically:

[0019] 1) The method of preparing xylose and arabinose from lignocellulosic biomass of the present invention uses ultra-low acid, which has little corrosive effect on equipment;

[0020] 2) The method for preparing xylose and arabinose from lignocellulosic biomass of the present invention has less xylose degradation products in the hydrolyzate, thus saving purification costs;

[0021] 3) The method of preparing xylose and arabinose from lignocellulosic biomass of the present invention is easy to scale up and suitable for large-scale industrial applications;

[0022] 4) The method of preparing xylose and arabinose from wood fiber biomass of the present invention does not require the use of organic solvents and is green, environmentally friendly and efficient. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is the SEM image of the dewaxed wheat straw powder in Example 1.

[0024] Figure 2 This is the SEM image of the solid residue in Example 1. DETAILED DESCRIPTION

[0025] The present invention will be further explained and illustrated below in conjunction with specific embodiments.

[0026] Embodiment 1:

[0027] A method for preparing xylose and arabinose using lignocellulosic biomass, comprising the following steps:

[0028] 1 g of 40-60 mesh dewaxed wheat straw powder and 20 mL of 1 g / L formic acid aqueous solution were added to a hydrothermal reactor, reacted at 170°C for 60 min, and then the hydrothermal reactor was quickly cooled to room temperature using running water. A G3 sand core funnel was used to separate the reaction liquid into solid and liquid to remove solid residues (rich in cellulose and lignin), thereby obtaining an aqueous solution containing xylose and arabinose.

[0029] According to the test, in this embodiment, the yield of xylose is 73.01%, the yield of arabinose is 100%, the yield of furfural is 0.01%, and the removal rate of hemicellulose is 82.02%.

[0030] Note:

[0031] The contents of xylose, arabinose and furfural in the aqueous solution containing xylose and arabinose are all determined by high performance liquid chromatography (HPLC). The calculation formula for the content of xylose / arabinose is as follows: y=0.0029x, wherein x is the peak area measured by HPLC, and y is the content of xylose / arabinose, in mg / L. The calculation formula for the content of furfural is as follows: b=0.0024a, wherein a is the peak area measured by HPLC, and b is the content of furfural, in mg / L.

[0032] Xylose yield (%) = mass of xylose in the aqueous solution containing xylose and arabinose / mass of xylose in the lignocellulosic biomass raw material × 100%;

[0033] Arabinose yield (%) = mass of arabinose in the aqueous solution containing xylose and arabinose / mass of arabinose in the lignocellulosic biomass raw material × 100%;

[0034] Furfural yield (%) = molar amount of furfural in the aqueous solution containing xylose and arabinose / total molar amount of xylose and arabinose in the lignocellulosic biomass raw material × 100%;

[0035] Hemicellulose removal rate (%) = (mass of hemicellulose in the lignocellulosic biomass raw material - mass of hemicellulose in the solid residue) / mass of hemicellulose in the lignocellulosic biomass raw material × 100%.

[0036] The scanning electron microscope (SEM) image of the dewaxed wheat straw powder in this example is as follows: Figure 1 The SEM image of the solid residue is shown in Figure 2 shown.

[0037] Depend on Figure 1 and Figure 2 It can be seen that the dewaxed wheat straw powder presents a dense and relatively complete surface structure, while the surface of the solid residue formed by hydrolyzing the dewaxed wheat straw powder in an aqueous formic acid solution becomes rough, and many wrinkles and holes appear on the fiber surface, and the surface is severely damaged. The reason is that hemicellulose is hydrolyzed into monosaccharides and enters the hydrolyzate.

[0038] Embodiment 2:

[0039] A method for preparing xylose and arabinose using lignocellulosic biomass, comprising the following steps:

[0040] 1 g of 60-80 mesh rice straw powder (undewaxed) and 30 mL of 1 g / L sulfuric acid aqueous solution were added to a hydrothermal reactor, reacted at 180°C for 30 min, and then the hydrothermal reactor was quickly cooled to room temperature using running water. A G3 sand core funnel was used to separate the reaction liquid into solid and liquid to remove the solid residue (rich in cellulose and lignin), thereby obtaining an aqueous solution containing xylose and arabinose.

[0041] According to the test, in this embodiment, the yield of xylose is 75.32%, the yield of arabinose is 100%, the yield of furfural is 0.10%, and the removal rate of hemicellulose is 85.67%.

[0042] Embodiment 3:

[0043] A method for preparing xylose and arabinose using lignocellulosic biomass, comprising the following steps:

[0044] 1 g of 100-120 mesh corn cob powder (undewaxed) and 10 mL of 0.75 g / L acetic acid aqueous solution were added to a hydrothermal reactor, reacted at 140° C. for 180 min, and then the hydrothermal reactor was quickly cooled to room temperature using running water. A G3 sand core funnel was used to separate the reaction liquid into solid and liquid to remove the solid residue (rich in cellulose and lignin), thereby obtaining an aqueous solution containing xylose and arabinose.

[0045] According to the test, in this embodiment, the yield of xylose is 59.51%, the yield of arabinose is 95.32%, the yield of furfural is 0, and the removal rate of hemicellulose is 63.29%.

[0046] Embodiment 4:

[0047] A method for preparing xylose and arabinose using lignocellulosic biomass, comprising the following steps:

[0048] 1 g of 80-100 mesh corn stalk powder (undewaxed) and 50 mL of 1 g / L p-toluenesulfonic acid-tartaric acid aqueous solution (the total concentration of p-toluenesulfonic acid and tartaric acid is 1 g / L, and the mass ratio of p-toluenesulfonic acid to tartaric acid is 1:1) were added to a hydrothermal reactor, reacted at 160°C for 120 min, and then the hydrothermal reactor was quickly cooled to room temperature using running water, and then a G3 sand core funnel was used to perform solid-liquid separation on the reaction solution to remove solid residues (rich in cellulose and lignin), thereby obtaining an aqueous solution containing xylose and arabinose.

[0049] According to the test, in this embodiment, the yield of xylose is 81.35%, the yield of arabinose is 100%, the yield of furfural is 0.09%, and the removal rate of hemicellulose is 88.67%.

[0050] Embodiment 5:

[0051] A method for preparing xylose and arabinose using lignocellulosic biomass, comprising the following steps:

[0052] 0.5 g of 20-40 mesh dewaxed corn cob powder, 0.5 g of 20-40 mesh dewaxed corn stalk powder and 15 mL of 0.5 g / L sulfuric acid-acetic acid aqueous solution (the total concentration of sulfuric acid and acetic acid is 0.5 g / L, and the mass ratio of sulfuric acid to acetic acid is 1:1) were added to a hydrothermal reactor, reacted at 150° C. for 240 min, and then the hydrothermal reactor was quickly cooled to room temperature using running water, and then a G3 sand core funnel was used to perform solid-liquid separation on the reaction solution to remove solid residues (rich in cellulose and lignin), thereby obtaining an aqueous solution containing xylose and arabinose.

[0053] According to the test, in this embodiment, the yield of xylose is 76.52%, the yield of arabinose is 100%, the yield of furfural is 0.06%, and the removal rate of hemicellulose is 84.56%.

[0054] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A method for preparing xylose and arabinose using lignocellulosic biomass, characterized in that: The method comprises the following steps: adding wood fiber biomass powder into ultra-low acid aqueous solution for hydrolysis, and then performing solid-liquid separation to remove solid residues to obtain an aqueous solution containing xylose and arabinose.

2. The method for preparing xylose and arabinose from lignocellulosic biomass according to claim 1, characterized in that: The wood fiber biomass powder is at least one of wheat straw powder, rice straw powder, corn straw powder, corn cob powder, bagasse, and wood powder.

3. The method for preparing xylose and arabinose using lignocellulosic biomass according to claim 1 or 2, characterized in that: The mesh number of the wood fiber biomass powder is 20 meshes to 120 meshes.

4. The method for preparing xylose and arabinose from lignocellulosic biomass according to claim 1, characterized in that: The solute in the ultra-low acid aqueous solution is at least one of formic acid, p-toluenesulfonic acid, oxalic acid, acetic acid, L-malic acid, malonic acid, mandelic acid, tartaric acid, caffeic acid, sulfuric acid, and phosphoric acid.

5. The method for preparing xylose and arabinose from lignocellulosic biomass according to claim 1 or 4, characterized in that: The concentration of the ultra-low acid aqueous solution is 0.25 g / L to 1 g / L.

6. The method for preparing xylose and arabinose from lignocellulosic biomass according to any one of claims 1, 2 and 4, characterized in that: The usage ratio of the wood fiber biomass powder and the ultra-low acid aqueous solution is 1g:10mL-50mL.

7. The method for preparing xylose and arabinose from lignocellulosic biomass according to any one of claims 1, 2 and 4, characterized in that: The hydrolysis is carried out at a temperature of 120°C to 180°C.

8. The method for preparing xylose and arabinose from lignocellulosic biomass according to claim 7, characterized in that: The hydrolysis time is 30 min to 240 min.

9. The method for preparing xylose and arabinose from lignocellulosic biomass according to any one of claims 1, 2 and 4, characterized in that: The hydrolysis is carried out in a hydrothermal reactor.

10. The method for preparing xylose and arabinose from lignocellulosic biomass according to any one of claims 1, 2 and 4, characterized in that: The solid-liquid separation method is filtration.

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