Stable, efficient and environment-friendly silver nanoparticle lactic acid bacteria biosynthesis method and application

By screening and optimizing strains and optimizing culture medium components, a stable and efficient biosynthesis method of lactic acid bacteria AgNPs was developed, which solved the efficiency and stability of lactic acid bacteria biosynthesis silver nanoparticles in the prior art, and applied it to improve composite antibacterial packaging, extending the shelf life of cold fresh meat and improving food safety guarantees.

CN119662740APending Publication Date: 2025-03-21ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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Patent Information

Application Number
CN202411838523.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-13
Publication Date
2025-03-21

AI Technical Summary

Technical Problem

The process of biosynthesis of silver nanoparticles of lactic acid bacteria in the prior art has problems such as complex purification, low conversion efficiency, unstable yield, high energy consumption and high cost, and the potential of lactic acid bacteria cells as nanofactories still needs further research and optimization.

Method used

Optimized strains were screened through nitrate reduction test, and the media components and synthesis conditions were optimized using the response surface method. A stable, efficient and environmentally friendly biosynthesis method of lactic acid bacteria AgNPs was developed, and applied to improved composite antibacterial packaging.

Benefits of technology

It has achieved efficient biosynthesis and stable yield of silver nanoparticles, and improved antibacterial packaging has effectively extended the shelf life of cold fresh meat and improved food safety guarantee.

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Abstract

The invention discloses a stable, efficient and environment-friendly silver nanoparticle lactic acid bacteria biosynthesis method and application, firstly, a nitrate reduction test is carried out to determine the ability of strains to reduce nitrate into nitrite, and optimized strains are screened from six lactic acid bacteria; then, a response surface method is used for optimizing culture medium components for nitrate reductase production, and synthesis conditions are optimized; and synthesizing the silver nanoparticles by using the screened optimized strains. Compared with physical and chemical methods, the lactobacillus synthesis of AgNPs has the advantages of cost effectiveness, environmental friendliness, low toxicity to human cells, easiness in large-scale production, no need of dangerous chemicals, high temperature and high pressure and the like. The invention provides an efficient, stable and environment-friendly method for biologically synthesizing silver nanoparticles by using lactic acid bacteria, the improvement of antibacterial packaging materials is a key strategy and method for controlling microorganisms of chilled meat putrefaction in China, and powerful technical support is provided for scientifically, systematically, efficiently and practically guaranteeing meat safety.
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