Detection device for density gradient centrifugal sample

Abstract

The invention provides a detection device for a density gradient centrifugal sample, and belongs to the technical field of detection devices. The device comprises a sample suction platform mechanism which is used for sucking layered samples to be detected in a centrifugal tube layer by layer and conveying the layered samples to a sample detection chamber of an optical detector mechanism; the optical detector mechanism is provided with a xenon lamp light source and a beam shrinking light path system and supports 200-1100 nm full-wavelength detection; and the automatic collecting platform mechanism is used for sub-packaging the samples detected by the optical detector mechanism. According to the invention, a separation system and a collection system are integrated into a continuous process, so that man-made interference is reduced, and the resolution ratio and efficiency are improved. The xenon lamp light source and the beam shrinking light path system are adopted, the detection precision and stability are improved based on the spectrophotometer principle, the detection wavelength can be freely selected, and the limitation of traditional dual wavelengths is broken through.

Description

Technical Field

[0001] The present invention relates to the technical field of detection devices, and particularly to a detection device for density gradient centrifugation samples. Background Art

[0002] Density gradient centrifugation is a basic and most commonly used separation method in biochemistry and life science research. It utilizes the differences in sedimentation coefficients of different substances. Under certain centrifugation speed or centrifugal force conditions, substances with different sedimentation coefficients are separated and form bands in different regions of the density gradient. Its typical applications include the separation of different density lipoproteins, the purification and separation of large cell particles, the separation of nucleic acids, the separation of subcellular particles, and the separation of biological membranes, etc. Commonly used density gradient preparation solutions include sucrose, cesium chloride, sodium bromide, etc. However, how to quickly, conveniently and accurately collect our target substances from the different density bands formed after density gradient centrifugation is still a problem to be solved at present.

[0003] Currently, there are three methods for collecting after density gradient centrifugation. One is to observe with the naked eye, slowly insert a needle from above into the liquid surface, penetrate into the target layer, and then manually suck it out; the second is to pierce the bottom of the centrifuge tube with a needle, and then collect the liquid, and later the flow rate can also be controlled for collection through improvement; however, due to the vertical movement of the gradient, these two methods will cause particle residues on part of the centrifuge tube wall, so the resolution is not high; and the third method is the fully automatic density gradient preparation and separation system of Biocomp Company. It solves the problem of particle residues on the centrifuge tube wall through the movement of the piston from top to bottom, and detects the signal of the collected sample by installing LED lights with corresponding wavelengths of UV 260nm and 280nm, which increases the resolution and accuracy of collection. However, currently, the signal collection wavelengths of Biocomp Company are only two light sources of 260nm and 280nm. It is very effective for collecting spectral substances with these two characteristic wavelengths, such as nucleic acids and proteins, but there are still great limitations. One is that its separation system and collection system are two independent systems, and the other is limited by the selection of two wavelengths, resulting in no optional signal collection methods for collecting some samples such as liposomes and large cell particles. Summary of the Invention

[0004] In view of this, to solve the technical problems in the prior art that the separation system and the collection system of the fully automatic density gradient preparation and separation system are two independent systems and only two wavelengths can be selected, resulting in no selectable signal acquisition method for the collection of some samples such as liposomes and large cell particles. The present invention provides a detection device for density gradient centrifugation samples, which integrates the separation system and the collection system into a continuous process, reduces human interference, and improves resolution and efficiency. By adopting a xenon light source and a beam-shrinking optical path system, based on the principle of a spectrophotometer, the detection accuracy and stability are improved, and the detection wavelength can be freely selected, breaking through the limitation of the traditional dual wavelength.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A detection device for density gradient centrifugation samples, comprising:

[0007] A sample aspiration platform mechanism for aspirating the layered samples to be detected in the centrifuge tube layer by layer and transporting them to the sample detection chamber of the optical detector mechanism;

[0008] An optical detector mechanism, which is configured with a xenon light source and a beam-shrinking optical path system, supporting full wavelength detection from 200nm to 1100nm;

[0009] An automatic collection platform mechanism for dispensing the samples detected by the optical detector mechanism.

[0010] Preferably, the sample aspiration platform mechanism includes:

[0011] A base, which is arranged on the platform of the sample aspiration platform mechanism;

[0012] A hydraulic support, which is arranged on the base;

[0013] A sliding table, which is slidably connected to the hydraulic support;

[0014] A fixed sleeve, which is arranged on the base for fixing the centrifuge tube;

[0015] A hydraulic rod, with a hydraulic suction head for aspirating the layered samples to be detected in the centrifuge tube at the lower end, and connected to the sliding table at the upper end;

[0016] A driving device for driving the sliding table to lift and lower along the extension direction of the hydraulic support.

[0017] Preferably, the driving device includes:

[0018] A lead screw mechanism, which has a lead screw;

[0019] A hydraulic pressure motor, which is connected to the lead screw through a coupling, and drives the sliding table to drive the hydraulic rod to lift and lower through the lead screw.

[0020] Preferably, the optical detector mechanism further includes:

[0021] A signal detector for collecting the ultraviolet spectral absorption signal of the sample.

[0022] Preferably, the automatic collection platform mechanism includes:

[0023] A collection needle for collecting the sample after detection in the sample detection chamber into the sample collection plate.

[0024] Preferably, the automatic collection platform mechanism further includes:

[0025] A drive system for driving the collection needle to move to the area where the sample collection plate is located.

[0026] Preferably, the drive system includes an X-axis drive system for driving the collection needle to move in the X-axis direction and / or a Y-axis drive system for driving the collection needle to move in the Y-axis direction.

[0027] Preferably, the X-axis drive system drives the Y-axis drive system to move in the X-axis direction, and the collection needle is arranged on the Y-axis drive system.

[0028] Preferably, the automatic collection platform mechanism further includes a waste liquid pool.

[0029] Preferably, the automatic collection platform mechanism further includes a radiator.

[0030] The present invention has the following beneficial effects compared with the prior art:

[0031] The present invention relates to a device for detecting density gradient centrifugation samples. The uniqueness of this device lies in that it ingeniously integrates the separation system and the collection system into a continuous process, thus significantly reducing the interference of human operation. This integrated design not only improves the resolution of the entire detection process but also greatly enhances the work efficiency. In addition, the device uses a xenon light source and a beam-shrinking optical path system, based on the principle of a spectrophotometer, further enhancing the detection accuracy and stability. Users can freely select different detection wavelengths according to their needs, which makes the device no longer limited by the limitations of traditional dual wavelengths, providing greater flexibility and higher accuracy for scientific research and experiments.

[0032] Based on the integration of the separation system and the collection system, the present invention innovatively combines the principle of a spectrophotometer, enables it to detect flowing samples through a beam-shrinking optical path, improves the method for detecting the flowing liquid path, integrates the separation system and the collection system into one system, and utilizes the optical path of ultraviolet-full wavelength detection. It can detect at any wavelength within the visible light wavelength range or perform a full wavelength scan on the liquid path to determine the characteristic absorption of substances, thereby making the collection method after density gradient centrifugation accurate, fast, and visual.

[0033] Other advantages of the present invention will be described in detail in the following specific implementation part. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 is a schematic diagram of the overall structure of the present invention;

[0035] Figure 2 is a schematic diagram of the sample aspiration platform mechanism of the present invention;

[0036] Figure 3 is a schematic diagram of the optical detector mechanism of the present invention;

[0037] Figure 4 is a schematic diagram of the automatic sample collection platform mechanism of the present invention;

[0038] Figure 5 is for Figure 3 a schematic diagram of the detection optical path of the sample detection chamber of the optical detector;

[0039] In the figure, 1 is the sample aspiration platform mechanism; 2 is the optical detector mechanism; 3 is the automatic sample collection platform mechanism; 11 is the hydraulic support; 12 is the lead screw mechanism, including 121 the lead screw, 122 the support end, and 123 the fixed end; 13 is the slide; 14 is the first connecting flange; 15 is the hydraulic rod, including 151 the hydraulic suction head; 16 is the coupling; 17 is the fixed sleeve; 18 is the liquid pressing motor; 19 is the liquid pressing motor mounting plate; 20 is the guide rail; 21 is the xenon light source; 22 is the beam-shrinking optical path system; 23 is the sample detection chamber; 24 is the signal detector; 31 is the Y-axis motor, including 311 the Y-axis motor shaft; 32 is the Y-axis guide rail; 33 is the first X-axis guide rail; 34 is the second connecting flange; 35 is the X-axis motor, including 351 the X-axis motor shaft; 36 is the second X-axis guide rail; 37 is the mounting plate; 38 is the connecting rod; 39 is the collection needle; 3010 is the waste liquid pool; 3011 is the sample collection plate; 3012 is the radiator; 3013 is the fixed sleeve. SPECIFIC EMBODIMENTS

[0040] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0041] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "upper", "lower", "inner", "outer", "top / bottom end", etc. is based on the orientation or positional relationship shown in the accompanying drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention.

[0042] In the description of the present invention, it should be noted that unless otherwise clearly specified and limited, the terms "installed", "provided with", "sheathed / connected", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0043] As Figure 1 shown, the present invention provides a detection device for density gradient centrifugation samples, including:

[0044] A sample aspiration platform mechanism 1 for aspirating the layered samples to be detected in the centrifuge tube layer by layer and transporting them to the sample detection chamber 23 of the optical detector mechanism 2. The main function of the sample aspiration platform mechanism 1 is to realize the automatic aspiration and transmission of samples. It can accurately aspirate the layered samples to be detected from the centrifuge tube layer by layer, and then transport these samples to the sample detection chamber 23 of the optical detector mechanism 2 for further analysis and detection. This process greatly reduces the complexity and error of manual operation, and improves the experimental efficiency and accuracy.

[0045] Optical detector mechanism 2, on which a xenon light source 21 and a beam-shrinking optical path system 22 are configured, supports full-wavelength detection from 200 nm to 1100 nm. It covers the spectral ranges of ultraviolet (characteristic absorption of nucleic acids and proteins), visible light (scattering of liposomes and cell clusters), and near-infrared (detection of specific markers). It is based on the principle of a spectrophotometer. Through the beam-shrinking optical path, it can detect flowing samples, improve the method for detecting the flowing liquid path, integrate the separation system and the collection system into one system, and utilize the optical path of ultraviolet-full wavelength detection. It can either select a certain wavelength within the visible light wavelength range for detection or perform a full-wavelength scan on the liquid path to determine the characteristic absorption of substances, thereby making the collection method after density gradient centrifugation accurate, fast, and visual.

[0046] The xenon light source 21 emits a continuous spectrum, penetrates the liquid path of the flowing sample detection chamber 23, and the signal detector captures the transmitted light signal in real time to calculate the absorbance or the intensity of scattered light.

[0047] When light of a specific wavelength passes through the flowing sample, the absorption intensity of the target substance to the light is proportional to its concentration (formula: A = ε·c·l, where A is the absorbance, ε is the molar extinction coefficient, c is the concentration, and l is the optical path length).

[0048] Among them, the beam-shrinking optical path system 22 is as Figure 5 shown. The xenon light source 21 uses the broadband composite light emitted by the xenon lamp. The beam-shrinking optical path realizes the separation of the beam wavelength based on a dispersion spectroscope, forming a reference optical path and a sample optical path that are parallel up and down. The beam first passes through the objective lens for beam shrinking and then enters the eyepiece for focusing. Among them, the reference optical path passes through air, and the sample optical path penetrates the sample to be detected flowing. Finally, the two groups of optical paths carry different-wavelength optical information and reach the detector synchronously. By comparing the attenuation or change characteristics of the light of a specific wavelength in the two paths, the analysis of the optical performance of the sample is realized. Here, the reference optical path is defaulted to detect air, and the sample optical path detects the density gradient stratified solution aspirated by the 1 sample aspiration platform mechanism.

[0049] Therefore, through the fluidization transformation of the spectrophotometer principle, the precise optical design of the beam-shrinking optical path, and the integration of the functions of the whole system, the present invention solves the three major technical bottlenecks of single wavelength, low sensitivity, and fragmented operation in the detection of density gradient centrifugation samples, realizes broad-spectrum, high-precision, and fully automatic detection and collection, and provides an efficient and reliable tool for biochemical separation experiments.

[0050] An automatic collection platform mechanism is used to subpackage the samples detected by the optical detector mechanism 2. It can effectively organize and manage the samples, ensure that each sample is processed according to a predetermined process, thereby improving the efficiency and accuracy of the whole detection process.

[0051] Such as Figure 2As shown, in the present invention, the sample aspiration platform mechanism 1 includes:

[0052] A base, which is arranged on the platform of the sample aspiration platform mechanism 1 and serves as the support foundation for the sample aspiration platform mechanism 1, the optical detector mechanism 2, and the automatic collection platform mechanism.

[0053] A hydraulic support 11, which is arranged on the base, supports the rigid frame of the entire sample aspiration platform mechanism 1, and is fixedly connected to the base.

[0054] A sliding table 13, which is slidably connected to the hydraulic support 11. Preferably, the sliding table 13 is slidably connected to the slide rail arranged on the hydraulic support 11.

[0055] A fixed sleeve 17, which is arranged on the base and is used to fix the centrifuge tube to ensure that the sample tube is vertical and stable during the aspiration process, and to avoid the liquid surface from tilting or shaking.

[0056] A hydraulic rod 15, whose lower end is carefully designed and equipped with a hydraulic suction head 151 capable of aspirating the layered sample to be detected in the centrifuge tube. This hydraulic suction head 151 can accurately aspirate the sample to ensure the integrity of the sample. The upper end of the hydraulic rod 15 is tightly connected to the sliding table 13 (preferably connected through a first connecting flange 14), ensuring the stability and precise movement of the hydraulic rod 15. In addition, the hydraulic rod 15 adopts a hollow pipe design, which enables the hydraulic rod 15 to effectively transport the aspirated layered sample to be detected to the sample detection chamber 23. In order to further improve the efficiency and accuracy of sample transmission, the inner wall of the hollow pipe is specially treated and made of a smooth material, such as a polytetrafluoroethylene coating, which can significantly reduce the residue of the liquid during transmission and ensure the purity of the sample and the accuracy of the detection result.

[0057] When designing the hydraulic suction head 151, its tip part is specially considered to ensure the best performance and safety. Usually, the tip is designed to be a blunt shape. The main purpose of this design is to avoid damaging the layered interface during the puncture process. The layered interface refers to the interface between different layers in some liquids or substances, and these layers need to be kept clear to avoid mixing or contamination. By adopting a blunt tip design, the damage to these interfaces can be effectively reduced, thus maintaining their integrity. In addition, the hydraulic suction head 151 can also be equipped with a micro pressure sensor, which can monitor the pressure change during the aspiration process in real time. This monitoring function is crucial for preventing excessive aspiration of liquid, because excessive liquid aspiration may cause the substances that should originally remain layered to mix, thus affecting the experimental results or the quality of the product. The built-in micro pressure sensor can provide pressure data in a timely manner, enabling the operator to adjust the aspiration force according to these data to ensure that the aspiration process is both effective and safe.

[0058] A driving device, which is designed to drive the slide table 13 to move up and down along the extending direction of the hydraulic support 11. In this way, it can effectively drive the hydraulic rod 15 and the hydraulic suction head 151 to move up and down together, so as to realize the suction work of the stratified sample to be detected.

[0059] In the present invention, the driving device includes:

[0060] A lead screw mechanism 12, which has a lead screw 121 and may also have a support end 122 and a fixed end 123. For example, Figure 2 in it, the hydraulic pressure motor is installed on the hydraulic pressure motor mounting plate 19. The lead screw mechanism 12 includes a lead screw 121, a support end 122, a fixed end 123 and a lead screw nut. One end of the lead screw is installed at the fixed end 123 to fix the lead screw, and the other end is installed at the support end 122 to support the lead screw. The support end and the fixed end are fixedly installed on the hydraulic pressure support 11. The hydraulic pressure motor is connected to the lead screw through a coupling 16. The lead screw nut is fixedly connected to the slide table 13. The slide table 13 is installed on the guide rail 20. The hydraulic pressure motor 18 drives the lead screw to rotate, driving the lead screw nut on the lead screw to move up and down, thereby driving the entire slide table to move up and down along the guide rail 20.

[0061] A hydraulic pressure motor 18, which is tightly connected to the lead screw 121 through a coupling 16, and then drives the slide table 13 through the lead screw 121, so that the hydraulic rod 15 can move up and down. The hydraulic pressure motor 18 can preferably be fixed on the base. In this process, the hydraulic pressure motor 18 serves as the main power source, and the coupling 16 located above it plays a key connecting role. The connection between the coupling 16 and the lead screw 121 enables the lead screw 121 to rotate smoothly under the drive of the hydraulic pressure motor 18. The rotation of the lead screw 121 is then converted into the linear movement of the slide table 13, thereby realizing the lifting of the hydraulic rod 15.

[0062] Such as Figure 3 shown, in the present invention, the optical detector mechanism 2 further includes:

[0063] A signal detector, which is used to collect the ultraviolet spectral absorption signals of the sample. The signal detector is an indispensable part of the optical detector mechanism 2 and is specifically used to collect the spectral absorption signals generated by the sample after being irradiated by ultraviolet light of a specific wavelength. By accurately detecting and analyzing these signals, the composition, concentration and other related characteristics of the sample can be accurately judged and measured.

[0064] Such as that of the optical detector mechanism 2 Figure 4 shown, in the present invention, the automatic collection platform mechanism includes:

[0065] The collection needle 39 is used to collect the tested sample in the sample detection chamber 23 into the sample collection plate 3011. Among them, the sample collection plate 3011 is a porous sample collection plate 3011. The collection needle 39 collects the tested sample in the sample detection chamber 23 and distributes it into the designated holes of the sample collection plate 3011. Such a collection needle 39 is particularly suitable for the porous sample collection plate 3011. During the sample detection process, the collection needle 39 can effectively take out the tested sample from the detection chamber and ensure that the sample can be accurately distributed into the pre-set holes on the sample collection plate 3011, so as to realize the orderly management and subsequent analysis of the sample.

[0066] In the present invention, the automatic collection platform mechanism further includes:

[0067] A driving system for driving the collection needle 39 to move to the area where the sample collection plate 3011 is located. This driving system is responsible for precise control and operation to drive the collection needle 39 to move to the area where the sample collection plate 3011 is located, ensuring that the collection needle 39 can accurately reach the predetermined position, so as to realize the precise collection of the sample.

[0068] In the present invention, the driving system includes an X-axis driving system for driving the collection needle 39 to move along the X-axis direction and / or a Y-axis driving system for driving the collection needle 39 to move along the Y-axis direction. These two driving systems work together to ensure that the collection needle 39 can be efficiently and accurately positioned and moved on a two-dimensional plane. The design and implementation of the X-axis driving system and the Y-axis driving system enable the entire driving system to meet the working requirements of high precision and high efficiency, thus playing a key role in the automated equipment and improving production efficiency and product quality.

[0069] In the present invention, the described X-axis driving system is responsible for driving the Y-axis driving system to move along the X-axis direction. Specifically, the Y-axis driving system is designed to be able to accurately position and move along the X-axis direction to ensure that it can operate according to the predetermined path and speed. In addition, in order to achieve the precise collection of the sample, the collection needle 39 is ingeniously arranged and fixed on the Y-axis driving system. Such a layout enables the collection needle 39 to move synchronously with the movement of the Y-axis driving system, so as to achieve precise sample collection in the X-axis direction.

[0070] As Figure 4 shown, in the present invention, a bracket is formed by the connecting rod 38 and the mounting plate 37 for mounting the X-axis driving system. The X-axis driving system uses the X-axis motor 35 as the power source, and the output end is connected to the X-axis motor shaft 351 (such as an X-axis lead screw). The first X-axis guide rail 33 and the second X-axis guide rail 36 are respectively arranged on both sides for providing a sliding guide rail for the Y-axis driving system.

[0071] The Y-axis drive system has a slider which is slidably connected to the guide rail on the X-axis. A Y-axis motor is installed on the slider, and the output end of the Y-axis motor is connected to the Y-axis motor shaft 24 signal detector; one end of a Y-axis lead screw (such as 311), and the other end of the Y-axis lead screw is slidably connected to the second connecting flange 34. The collection needle 39 is installed on the second connecting flange 34. The Y-axis drive system can drive the collection needle 39 to move along the Y-axis guide rail 32 on the Y-axis through the Y-axis lead screw.

[0072] In the present invention, the automatic collection platform mechanism further includes a waste liquid pool 3010. During the aliquoting interval or when replacing samples, the collection needle 39 discharges non-target liquids into the waste liquid pool 3010 to avoid contaminating the sample collection plate 3011. This solves the core problems of inefficient waste liquid treatment and high pollution risk in traditional collection methods, significantly improves the reliability and safety of the fully automatic detection-collection system, and provides key technical support for the standardization and large-scale operation of biomedical experiments.

[0073] In the present invention, the automatic collection platform mechanism further includes a radiator 3012, whose main function is to quickly export heat to ensure the stable operation of the entire platform and extend the service life of the equipment. The design of the radiator 3012 takes into account the heat conduction efficiency and is usually made of high thermal conductivity materials such as aluminum alloy or copper to achieve the best heat dissipation effect. In addition, the radiator 3012 may be equipped with a fan or other cooling devices to enhance air flow and further improve the heat dissipation performance. In some advanced applications, the radiator 3012 may also integrate an intelligent temperature control system that can automatically adjust the cooling intensity according to the heat generated during the operation of the platform, thus taking into account both performance and energy efficiency.

[0074] The installation method and usage method of the present invention will be described below in conjunction with specific embodiments as follows:

[0075] As Figure 1 shown, first, this device is divided into three major mechanisms, namely the sample aspiration platform mechanism 1, the optical detector mechanism 2, and the automatic sample collection platform mechanism 3. Details can be seen in Figure 1 .

[0076] As Figure 2As shown in the figure, it is a schematic diagram of the sample suction platform mechanism 1 of this device. First, place the sample tube after density gradient centrifugation in the fixed sleeve 17 for fixation. The fixed sleeve 17 is fixed on the fixed sleeve 3013 of the base. A coupling 16 is arranged above the liquid pressing motor 18. The lead screw of the lead screw mechanism 12 is connected to the liquid pressing motor 18 through the coupling 16. Then, during the liquid pressing process, under the action of the liquid pressing motor 18, the lead screw mechanism 12 moves downward. At the same time, a connecting flange is connected to the lead screw mechanism 12. A support end 122 is provided at the upper end, and a fixed end 123 is provided at the lower end. The connecting flange is connected to the hydraulic rod 15. A hydraulic suction head 151 is provided at the lower end of the hydraulic rod 15. Under the action of the liquid pressing motor 18, the hydraulic suction head 151 moves downward. The layered samples in the sample tube are gradually sucked out to the sample detection chamber 23 of the optical detector mechanism 2 through the hydraulic suction head 151 and the hydraulic rod 15 layer by layer.

[0077] As Figure 3 shown in the figure, it is a schematic diagram of the optical detector mechanism 2 of this device. Under the action of the sample suction platform mechanism 1, the samples stratified after density gradient centrifugation flow into the sample detection pool respectively. At the same time, after the xenon light source 21 passes through the beam-shrinking optical path system 22, according to the different ultraviolet absorption properties of the samples to be detected, in Figure 5 the schematic diagram of the beam-shrinking detection optical path shown in the figure, the detection of the flowing samples can be carried out at any wavelength selected between 200 nm and 1100 nm. The ultraviolet spectral absorption signals of the samples are collected by the signal detector. The samples after flowing through the sample detection pool then enter the mechanism automatic sample collection platform mechanism 3 immediately.

[0078] As Figure 4 shown in the figure, it is a schematic diagram of the automatic sample collection platform mechanism 3 of this device. The liquid pressing motor 18 drives the liquid pressing rod to move downward through the lifting mechanism. The samples in the centrifuge tube, under the action of pressure, successively pass through the light source detection system and the collection device along the collection pipeline in the horn head, and finally reach the position of the collection needle 39. The samples are collected into the sample collection plate 3011 according to the volume or the number of sub-packaging tubes. The collection needle 39 can move along the X-axis guide rail and the Y-axis guide rail 32 respectively under the action of the X-axis motor 35 and the Y-axis motor 31. The samples are sub-packed into the corresponding holes of the sample collection plate 3011 through the displacement of the collection needle 39. A radiator 3012 is provided below the automatic sample collection platform mechanism 3, which can dissipate heat for the whole device.

[0079] The above; only the preferred specific implementation modes of the present invention; but the protection scope of the present invention is not limited thereto; any person skilled in the art within the technical scope disclosed by the present invention; according to the technical scheme and its improvement concept of the present invention, making equivalent replacements or changes; should be covered by the protection scope of the present invention.

Claims

1. A detection device for density gradient centrifugation samples, characterized in that, Comprising: A sample aspiration platform mechanism for aspirating the layered samples to be detected in the centrifuge tube layer by layer and transporting them to the sample detection chamber of the optical detector mechanism; An optical detector mechanism configured with a xenon light source and a beam-shrinking optical path system, supporting full-wavelength detection from 200nm to 1100nm; An automatic collection platform mechanism for dispensing the samples after being detected by the optical detector mechanism.

2. The detection device for density gradient centrifugation samples according to claim 1, wherein, The sample aspiration platform mechanism includes: A base disposed on the platform of the sample aspiration platform mechanism; A hydraulic support disposed on the base; A sliding table slidably connected to the hydraulic support; A fixed sleeve disposed on the base for fixing the centrifuge tube; A hydraulic rod having a hydraulic suction head at the lower end for aspirating the layered samples to be detected in the centrifuge tube, and the upper end is connected to the sliding table; A driving device for driving the sliding table to lift along the extending direction of the hydraulic support.

3. The detection device for density gradient centrifugation samples according to claim 2, characterized in that, The driving device includes: A lead screw mechanism having a lead screw; A hydraulic pressure motor connected to the lead screw through a coupling, and driving the sliding table to drive the hydraulic rod to lift through the lead screw.

4. The detection device for density gradient centrifugation samples according to claim 1, wherein, The optical detector mechanism further includes: A signal detector for collecting the ultraviolet spectral absorption signals of the samples.

5. The detection device for density gradient centrifugation samples according to claim 1, wherein, The automatic collection platform mechanism includes: A collection needle for collecting the detected samples in the sample detection chamber into the sample collection plate.

6. The detection device for density gradient centrifugation samples according to claim 5, wherein, The automatic collection platform mechanism further includes: A driving system for driving the collection needle to move to the area where the sample collection plate is located.

7. The detection device for density gradient centrifugation samples according to claim 6, characterized in that, The driving system includes an X-axis driving system for driving the collection needle to move along the X-axis direction and / or a Y-axis driving system for driving the collection needle to move along the Y-axis direction.

8. A detection device for density gradient centrifugation samples according to claim 7, characterized in that, The X-axis driving system drives the Y-axis driving system to move along the X-axis direction, and the collection needle is disposed on the Y-axis driving system.

9. The detection device for density gradient centrifugation samples according to claim 1, wherein The automatic collection platform mechanism further includes a waste liquid pool.

10. A detection device for density gradient centrifugation samples according to any one of claims 1-9, characterized in that, The automatic collection platform mechanism further includes a radiator.

Images